MD15G1229500.v1.1

Glutamate receptor

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
Physical Location & Seq
Reverse (-)
18808196 .. 18810486
2291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1229500.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGTTTAATGGTGGCAGCCAGCTGCTCCAAAGCATTTTAGAGGTTAATACAACTGGATTAACCGGGCAAATTAAGTTTACTCCAGACGGAAATTTGATTAATCCTGCATTTGAAGTGATCAATGTGATCGGCACAGGGACTAGGACTATTGGTTATTGGTCCAATTCTTCTGGCCTGTCGCTTGATCCACCTGATAACGTCATCTGGCCCGGACAAACAACTCAGAAGCCTCGCGGGTGGGTTTTCCCCGACAACGGAAGAAAGCTCAGGATTGGAGTCCCCGATCGAGGTACCTTCCGCGAATTTGTAGGCGTAAAAGGCACTGAGTTCACTGGCTATTGCATTGAGGTCTTTCAAGCCGCCTTAAATGAGTTACCATATGGTGTCCCATACAAGTTTGTAGCTTTCGGGGATGGTATAACAAACGCTGAAAGTAAGGAACTTCTGCACAGGGTCCAAATTGGTGAGTTGGATGGTGTGGTGGGAGATATTACAATCACCACTAGCAGAACAAAGATGGTGGATTTCACACAGCCATACATTGAGTCTGGTCTAGTTGTGGTTGCTCCAATTAGGAAGACGAACTCTAGTGCTTGGGCATTTTTGAGGCCTTTTAGTCCAATGATGTGGGGTGTCACAGGCATTTTCTTCCTAGTTGTGGGAGCAGTTGTTTGGATTTTGGAGCGTCGGACCAACGAGGATTTTCGAGGCCGTCCGAGAAGACAATTTGTCACAATTATTTGGTTTAGCTTTTCAACTCTGTTCTTCTCCCAGAATGAAAAAACCGGCAGCACGCTTGGTCGATTTGTGCTTATCATATGGCTATTTGTGGTTCTAATAATCAACTCAAGCTACATTGCTAGACTGACATCAATCCTCACTGTCGAGCAGCTTTCTTCACCTGTTAAAGGGATTGAAAGTCTGGTAAACGGCAATGATCCCATTGGTTTCCCAAGGGGTTCGTTCGCTGAAAACTACTTAACCGATGAGCTGAACATACACAAGTCAAGACTTGTGCCTCTTAACTCCCCAGAAGAATATGAGAAGGCCTTGAAAGATGGTCCTAGTGCAGGCGGTGTTGCTGCTGTGATTGATGAGCGTGCATACATGGAGCTCTTCCTCTCCGTTCGATGTGGATATAGTATAGTAGGTCAAGAATTCACCAAAATGGGATGGGGTTTCGCCTTTCCAAGGGACTCGCCTCTTGCAATTGACATGTCAACTGCGGTGTTGAAACTATCGGAGAAGGGAGATCTTCAAAAGATTCATGACAAATGGCTGAAGAAAAGTGCTTGCAGTGCAGAAGGAGCAAAGCAAGCCGTAGACCGTCTTTCGTTGAGTAGCTTTTGGGGCCTCTTTCTGCTGAGCGGCATAGCTTGCTTTCTGGCTCTTGTTCTTCATGTTCTTCGCATGGTGCACCAGTACTACAGGCATTATGATTCCGACTGCGAGAGCTCACAAGCGAAAAGCCTTCGATCCTTCATCTCGTTTGTAAATAAAAGGGAAGAGGAAGTGAAAAGCAGAACCAACAGGAGGACTGAGAAGGCTTCAAATAAAATAGTGCATGATCAAGATAGTAGTTCCATTGATGGTTTGAGTCAAAGTGTTTAA

Protein Analysis

537

Amino Acids

59.69

Weight (kDa)

8.42

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 2 - 44 1.4e-07 Receptor family ligand binding region
SBP_bac_3 PF00497 101 - 428 8.7e-13 Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd PF10613 108 - 189 6.1e-08 Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan PF00060 208 - 460 1.8e-34 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 290
AccBSI CCGCTC 1 cut(s) 1368
AccI GTMKAC 1 cut(s) 1323
AccII CGCG 2 cut(s) 234, 300
AciI CCGC 6 cut(s) 234, 298, 360, 1074, 1226, 1368
AclWI GGATC 3 cut(s) 179, 932, 1470
AcsI RAATTY 3 cut(s) 91, 302, 1157
AcuI CTGAAG 1 cut(s) 1301
AfaI GTAC 2 cut(s) 292, 1424
AfiI CCNNNNNNNGG 7 cut(s) 254, 287, 658, 908, 1070, 1191, 1533
AflIII ACRYGT 1 cut(s) 1215
AgsI TTSAA 8 cut(s) 113, 356, 756, 917, 1054, 1234, 1259, 1551
AhdI GACNNNNNGTC 1 cut(s) 728
Alw21I GWGCWC 3 cut(s) 1116, 1419, 1457
Alw44I GTGCAC 1 cut(s) 1415
AlwI GGATC 3 cut(s) 179, 932, 1470
AoxI GGCC 6 cut(s) 172, 206, 608, 709, 1047, 1351
ApaLI GTGCAC 1 cut(s) 1415
ApeKI GCWGC 5 cut(s) 15, 22, 789, 889, 1082
ApoI RAATTY 3 cut(s) 91, 302, 1157
AseI ATTAAT 1 cut(s) 99
Asp718I GGTACC 1 cut(s) 290
AspS9I GGNCC 6 cut(s) 159, 207, 454, 690, 1061, 1351
AsuC2I CCSGG 2 cut(s) 64, 210
AsuHPI GGTGA 4 cut(s) 476, 490, 891, 1153
AvaII GGWCC 4 cut(s) 159, 454, 690, 1061
BaeGI GKGCMC 1 cut(s) 1419
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 2 cut(s) 1116, 1457
BbsI GAAGAC 2 cut(s) 584, 727
Bbv12I GWGCWC 3 cut(s) 1116, 1419, 1457
BbvI GCAGC 5 cut(s) 9, 27, 801, 901, 1069
BccI CCATC 6 cut(s) 407, 467, 511, 1052, 1167, 1583
BceAI ACGGC 3 cut(s) 696, 946, 1304
BclI TGATCA 2 cut(s) 117, 1567
BcnI CCSGG 2 cut(s) 64, 210
BfaI CTAG 7 cut(s) 141, 504, 554, 588, 653, 861, 1065
BfmI CTRYAG 1 cut(s) 1426
BglII AGATCT 1 cut(s) 1252
BisI GCNGC 7 cut(s) 16, 23, 360, 790, 890, 1083, 1369
BlpI GCTNAGC 1 cut(s) 1364
BlsI GCNGC 7 cut(s) 17, 24, 361, 791, 891, 1084, 1370
BmcAI AGTACT 1 cut(s) 1424
Bme1390I CCNGG 2 cut(s) 64, 210
Bme18I GGWCC 4 cut(s) 159, 454, 690, 1061
BmeRI GACNNNNNGTC 1 cut(s) 728
BmgT120I GGNCC 6 cut(s) 159, 207, 454, 690, 1061, 1351
BmiI GGNNCC 3 cut(s) 292, 455, 1352
BmrFI CCNGG 2 cut(s) 64, 210
BpiI GAAGAC 2 cut(s) 584, 727
BpmI CTGGAG 1 cut(s) 66
Bpu10I CCTNAGC 1 cut(s) 266
Bpu1102I GCTNAGC 1 cut(s) 1364
BpuEI CTTGAG 1 cut(s) 832
BpuMI CCSGG 2 cut(s) 64, 210
BsaJI CCNNGG 2 cut(s) 953, 1190
BsaXI ACNNNNNCTCC 2 cut(s) 654, 684
Bsc4I CCNNNNNNNGG 7 cut(s) 254, 287, 658, 908, 1070, 1191, 1533
Bse118I RCCGGY 1 cut(s) 785
Bse1I ACTGG 3 cut(s) 58, 337, 1420
Bse3DI GCAATG 2 cut(s) 855, 940
BseDI CCNNGG 2 cut(s) 953, 1190
BseGI GGATG 3 cut(s) 418, 478, 1178
BseLI CCNNNNNNNGG 7 cut(s) 254, 287, 658, 908, 1070, 1191, 1533
BseMI GCAATG 2 cut(s) 855, 940
BseMII CTCAG 5 cut(s) 236, 280, 315, 1355, 1530
BseNI ACTGG 3 cut(s) 58, 337, 1420
BseSI GKGCMC 1 cut(s) 1419
BseXI GCAGC 5 cut(s) 9, 27, 801, 901, 1069
BsgI GTGCAG 3 cut(s) 431, 1089, 1320
Bsh1236I CGCG 2 cut(s) 234, 300
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 6 cut(s) 174, 208, 610, 711, 1049, 1353
BshNI GGYRCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 3 cut(s) 1116, 1419, 1457
BsiSI CCGG 3 cut(s) 63, 210, 786
BslFI GGGAC 4 cut(s) 151, 263, 371, 1208
BslI CCNNNNNNNGG 7 cut(s) 254, 287, 658, 908, 1070, 1191, 1533
BsmFI GGGAC 4 cut(s) 151, 263, 371, 1208
BsnI GGCC 6 cut(s) 174, 208, 610, 711, 1049, 1353
Bsp1286I GDGCHC 3 cut(s) 1116, 1419, 1457
Bsp143I GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
Bsp1720I GCTNAGC 1 cut(s) 1364
BspACI CCGC 6 cut(s) 234, 298, 360, 1074, 1226, 1368
BspANI GGCC 6 cut(s) 174, 208, 610, 711, 1049, 1353
BspCNI CTCAG 5 cut(s) 235, 279, 316, 1356, 1531
BspFNI CGCG 2 cut(s) 234, 300
BspHI TCATGA 1 cut(s) 1267
BspLI GGNNCC 3 cut(s) 292, 455, 1352
BspPI GGATC 3 cut(s) 179, 932, 1470
BspQI GCTCTTC 1 cut(s) 1121
BspT107I GGYRCC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 1368
BsrDI GCAATG 2 cut(s) 855, 940
BsrFI RCCGGY 1 cut(s) 785
BsrI ACTGG 3 cut(s) 58, 337, 1420
BssAI RCCGGY 1 cut(s) 785
BssECI CCNNGG 2 cut(s) 953, 1190
BssMI GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
BssT1I CCWWGG 2 cut(s) 953, 1190
Bst4CI ACNGT 2 cut(s) 883, 1328
Bst6I CTCTTC 2 cut(s) 1121, 1500
BstAPI GCANNNNNTGC 1 cut(s) 1377
BstC8I GCNNGC 7 cut(s) 20, 794, 1072, 1101, 1294, 1317, 1378
BstDEI CTNAG 5 cut(s) 222, 266, 324, 1364, 1539
BstENI CCTNNNNNAGG 3 cut(s) 906, 1068, 1189
BstF5I GGATG 3 cut(s) 418, 478, 1178
BstFNI CGCG 2 cut(s) 234, 300
BstKTI GATC 8 cut(s) 120, 129, 187, 286, 940, 1255, 1478, 1570
BstMBI GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 6 cut(s) 318, 1298, 1307, 1316, 1350, 1377
BstNSI RCATGY 1 cut(s) 1219
BstSCI CCNGG 2 cut(s) 62, 208
BstSFI CTRYAG 1 cut(s) 1426
BstSLI GKGCMC 1 cut(s) 1419
BstUI CGCG 2 cut(s) 234, 300
BstV1I GCAGC 5 cut(s) 9, 27, 801, 901, 1069
BstV2I GAAGAC 2 cut(s) 584, 727
BstX2I RGATCY 1 cut(s) 1252
BstXI CCANNNNNNTGG 1 cut(s) 627
BstYI RGATCY 1 cut(s) 1252
BsuRI GGCC 6 cut(s) 174, 208, 610, 711, 1049, 1353
BtsCI GGATG 3 cut(s) 418, 478, 1178
BtsI GCAGTG 1 cut(s) 1303
BtsIMutI CAGTG 4 cut(s) 321, 330, 879, 1303
Cac8I GCNNGC 7 cut(s) 20, 794, 1072, 1101, 1294, 1317, 1378
CciI TCATGA 1 cut(s) 1267
Cfr10I RCCGGY 1 cut(s) 785
Cfr13I GGNCC 6 cut(s) 159, 207, 454, 690, 1061, 1351
CseI GACGC 1 cut(s) 674
Csp6I GTAC 2 cut(s) 291, 1423
CspCI CAANNNNNGTGG 2 cut(s) 501, 536
CviAII CATG 6 cut(s) 1108, 1216, 1268, 1400, 1411, 1565
CviQI GTAC 2 cut(s) 291, 1423
DdeI CTNAG 5 cut(s) 222, 266, 324, 1364, 1539
DpnI GATC 8 cut(s) 119, 128, 186, 285, 939, 1254, 1477, 1569
DpnII GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
DriI GACNNNNNGTC 1 cut(s) 728
Eam1104I CTCTTC 2 cut(s) 1121, 1500
Eam1105I GACNNNNNGTC 1 cut(s) 728
EarI CTCTTC 2 cut(s) 1121, 1500
Ecl136II GAGCTC 2 cut(s) 1114, 1455
Eco130I CCWWGG 2 cut(s) 953, 1190
Eco147I AGGCCT 2 cut(s) 610, 1049
Eco24I GRGCYC 2 cut(s) 1116, 1457
Eco47I GGWCC 4 cut(s) 159, 454, 690, 1061
Eco53kI GAGCTC 2 cut(s) 1114, 1455
Eco57I CTGAAG 1 cut(s) 1301
EcoICRI GAGCTC 2 cut(s) 1114, 1455
EcoNI CCTNNNNNAGG 3 cut(s) 906, 1068, 1189
EcoO109I RGGNCCY 1 cut(s) 1351
EcoRI GAATTC 1 cut(s) 1157
EcoT14I CCWWGG 2 cut(s) 953, 1190
EcoT38I GRGCYC 2 cut(s) 1116, 1457
ErhI CCWWGG 2 cut(s) 953, 1190
FaeI CATG 6 cut(s) 1111, 1219, 1271, 1403, 1414, 1568
FaqI GGGAC 4 cut(s) 151, 263, 371, 1208
FatI CATG 6 cut(s) 1107, 1215, 1267, 1399, 1410, 1564
FauI CCCGC 1 cut(s) 227
FauNDI CATATG 2 cut(s) 379, 818
FbaI TGATCA 2 cut(s) 117, 1567
FblI GTMKAC 1 cut(s) 1323
Fnu4HI GCNGC 7 cut(s) 16, 23, 360, 790, 890, 1083, 1369
FokI GGATG 3 cut(s) 425, 485, 1185
FriOI GRGCYC 2 cut(s) 1116, 1457
Fsp4HI GCNGC 7 cut(s) 16, 23, 360, 790, 890, 1083, 1369
FspBI CTAG 7 cut(s) 141, 504, 554, 588, 653, 861, 1065
GluI GCNGC 7 cut(s) 16, 23, 360, 790, 890, 1083, 1369
GsuI CTGGAG 1 cut(s) 66
HaeIII GGCC 6 cut(s) 174, 208, 610, 711, 1049, 1353
HapII CCGG 3 cut(s) 63, 210, 786
HgaI GACGC 1 cut(s) 674
Hin1II CATG 6 cut(s) 1111, 1219, 1271, 1403, 1414, 1568
HincII GTYRAC 1 cut(s) 1221
HindII GTYRAC 1 cut(s) 1221
HinfI GANTC 6 cut(s) 276, 545, 1196, 1264, 1439, 1597
HpaII CCGG 3 cut(s) 63, 210, 786
HphI GGTGA 4 cut(s) 476, 490, 891, 1153
Hpy166II GTNNAC 6 cut(s) 78, 330, 928, 1221, 1324, 1417
Hpy188I TCNGA 5 cut(s) 225, 690, 717, 1243, 1444
Hpy188III TCNNGA 6 cut(s) 83, 268, 1008, 1154, 1268, 1571
Hpy8I GTNNAC 6 cut(s) 78, 330, 928, 1221, 1324, 1417
Hpy99I CGWCG 1 cut(s) 690
HpyAV CCTTC 7 cut(s) 304, 1039, 1240, 1298, 1481, 1489, 1537
HpyCH4III ACNGT 2 cut(s) 883, 1328
HpyCH4IV ACGT 1 cut(s) 198
HpyF10VI GCNNNNNNNGC 6 cut(s) 318, 1298, 1307, 1316, 1350, 1377
HpyF3I CTNAG 5 cut(s) 222, 266, 324, 1364, 1539
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 6 cut(s) 1111, 1219, 1271, 1403, 1414, 1568
KpnI GGTACC 1 cut(s) 294
Ksp22I TGATCA 2 cut(s) 117, 1567
Kzo9I GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
LguI GCTCTTC 1 cut(s) 1121
LmnI GCTCC 6 cut(s) 30, 571, 662, 682, 1111, 1307
Lsp1109I GCAGC 5 cut(s) 9, 27, 801, 901, 1069
MaeI CTAG 7 cut(s) 141, 504, 554, 588, 653, 861, 1065
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 3 cut(s) 372, 634, 730
MalI GATC 8 cut(s) 119, 128, 186, 285, 939, 1254, 1477, 1569
MbiI CCGCTC 1 cut(s) 1368
MboI GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 1 cut(s) 1252
MhlI GDGCHC 3 cut(s) 1116, 1419, 1457
MlyI GAGTC 4 cut(s) 285, 554, 1190, 1606
MmeI TCCRAC 3 cut(s) 450, 668, 1467
MslI CAYNNNNRTG 1 cut(s) 1166
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 3 cut(s) 63, 210, 786
MspR9I CCNGG 2 cut(s) 64, 210
MunI CAATTG 1 cut(s) 1209
MvnI CGCG 2 cut(s) 234, 300
MwoI GCNNNNNNNGC 6 cut(s) 318, 1298, 1307, 1316, 1350, 1377
NciI CCSGG 2 cut(s) 64, 210
NdeI CATATG 2 cut(s) 379, 818
NdeII GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
NlaIII CATG 6 cut(s) 1111, 1219, 1271, 1403, 1414, 1568
NlaIV GGNNCC 3 cut(s) 292, 455, 1352
NmuCI GTSAC 2 cut(s) 634, 730
NspI RCATGY 1 cut(s) 1219
PagI TCATGA 1 cut(s) 1267
PceI AGGCCT 2 cut(s) 610, 1049
PciI ACATGT 1 cut(s) 1215
PciSI GCTCTTC 1 cut(s) 1121
PfeI GAWTC 2 cut(s) 1264, 1439
PkrI GCNGC 7 cut(s) 17, 24, 361, 791, 891, 1084, 1370
Ple19I CGATCG 1 cut(s) 286
PleI GAGTC 4 cut(s) 284, 553, 1190, 1605
PpsI GAGTC 4 cut(s) 284, 553, 1190, 1605
PscI ACATGT 1 cut(s) 1215
PshBI ATTAAT 1 cut(s) 99
Psp124BI GAGCTC 2 cut(s) 1116, 1457
PspN4I GGNNCC 3 cut(s) 292, 455, 1352
PspPI GGNCC 6 cut(s) 159, 207, 454, 690, 1061, 1351
PsuI RGATCY 1 cut(s) 1252
PvuI CGATCG 1 cut(s) 286
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 2 cut(s) 292, 1424
RsaNI GTAC 2 cut(s) 291, 1423
RseI CAYNNNNRTG 1 cut(s) 1166
SacI GAGCTC 2 cut(s) 1116, 1457
SapI GCTCTTC 1 cut(s) 1121
SatI GCNGC 7 cut(s) 16, 23, 360, 790, 890, 1083, 1369
Sau3AI GATC 8 cut(s) 117, 126, 184, 283, 937, 1252, 1475, 1567
Sau96I GGNCC 6 cut(s) 159, 207, 454, 690, 1061, 1351
ScaI AGTACT 1 cut(s) 1424
SchI GAGTC 4 cut(s) 285, 554, 1190, 1606
ScrFI CCNGG 2 cut(s) 64, 210
SduI GDGCHC 3 cut(s) 1116, 1419, 1457
SfcI CTRYAG 1 cut(s) 1426
SinI GGWCC 4 cut(s) 159, 454, 690, 1061
SmiMI CAYNNNNRTG 1 cut(s) 1166
SmlI CTYRAG 1 cut(s) 847
SmoI CTYRAG 1 cut(s) 847
SseBI AGGCCT 2 cut(s) 610, 1049
SsiI CCGC 6 cut(s) 234, 298, 360, 1074, 1226, 1368
SspMI CTAG 7 cut(s) 141, 504, 554, 588, 653, 861, 1065
SstI GAGCTC 2 cut(s) 1116, 1457
StuI AGGCCT 2 cut(s) 610, 1049
StyD4I CCNGG 2 cut(s) 62, 208
StyI CCWWGG 2 cut(s) 953, 1190
TaaI ACNGT 2 cut(s) 883, 1328
TaiI ACGT 1 cut(s) 201
TaqI TCGA 6 cut(s) 286, 706, 802, 885, 1129, 1474
TatI WGTACW 1 cut(s) 1422
TauI GCSGC 2 cut(s) 362, 1371
TfiI GAWTC 2 cut(s) 1264, 1439
TscAI CASTG 4 cut(s) 328, 337, 886, 1303
TseFI GTSAC 2 cut(s) 634, 730
TseI GCWGC 5 cut(s) 15, 22, 789, 889, 1082
Tsp45I GTSAC 2 cut(s) 634, 730
TspDTI ATGAA 4 cut(s) 792, 1256, 1388, 1471
TspGWI ACGGA 3 cut(s) 102, 270, 1114
TspRI CASTG 4 cut(s) 328, 337, 886, 1303
VneI GTGCAC 1 cut(s) 1415
VpaK11BI GGWCC 4 cut(s) 159, 454, 690, 1061
VspI ATTAAT 1 cut(s) 99
XagI CCTNNNNNAGG 3 cut(s) 906, 1068, 1189
XapI RAATTY 3 cut(s) 91, 302, 1157
XceI RCATGY 1 cut(s) 1219
XmiI GTMKAC 1 cut(s) 1323
XspI CTAG 7 cut(s) 141, 504, 554, 588, 653, 861, 1065
ZrmI AGTACT 1 cut(s) 1424
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.