Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001053

Physical Location & Seq

Genomic Coordinates Reverse (-)

18796980 .. 18800813

Gene Structure 3834 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1229400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2805 bp

ATGTCCTTTCGTAGTTTGAATATCAACATGAATCTAGCCTTGCTTGTGTTATTTATGGTTTTCTACAAAGGTTCTTCTTCTAGTGGACTCAGTACTACTACTAATGTCACTGCAAGACCTGATGTTGTGAACATTGGCGCGATTTTCACTTTCGATTCTGTCATTGGAAAAATCGCAAAACTCGCAATAACCCTTGCAGTTGAAGATGTCAATTCCAATCCAGAAATTCTCAATGGAACAAAGCTGACACTTAAAATGCAAAACACCAAATCCAGTGACTTTCTAGGCATCATTGAAGCTTTGCAGTTCACGGAGAACGACACGGTGGCCATAATTGGTCCCCAATACTCAGCAACAGCTCACGTAATCTCACACATCGCCGATGAGCTAAAAGTCCCTTTACTATCATTTGCCGCAACAGACCCAACTCTGTCTCCAAATCAGTTCCCGTTCTTTGTTCGAACAACGAGGAGTGACCTCTTTCAGATGGCTGCAGTAGCAGACCTTGTTGGCTACTATGAGTGGAGAGATGTCATAGCAATATATGTAAATGATGACTTCGGGCGGAATGCCATTGCTGCCTTGGGGGACAAGCTATCCGTAAAGCGCTGTAAAATTTCGTACAAGGTACCTCTGAGCCCCAAGACAACCAAAGATGAAATCAGGAAGGCACTGATTAGTGTGTCTTTAATGGAGTCTAGGATTGTGATCCTGCACATTTATACTAGTTGGGGTTTACAAGTGCTCACCGAAGCGCAAAGCCAAATGATGATGTCGAGTCCATACGTGTGGATTGCTACTGATTGGTTTTCTACTATAATAGATTCAGATCCTTCCTTGCCTTCTGCAAAAACAGATGACATGCAAGGGGTTCTCACATTGAAGATGTATACACCAGAATCAGAACTCAAAAAGAAGTTTAAATTGCGGTGGAGCAACTTAACTGGCACGAGGAGGGTGAATGGCTCTTCTTTTGGGTTAAATACGTATGGTCTATATGCCTATGATTCTGTATGGCTTCTTGCTCATGCCCTTGACTCATTTTTCGCTAATGGAGGAAATATTTCTTTTGCAAATGACTCAAATTTGCACAAATTACGAGGAGGGAAGTTGAACCTTGATGCTTTAAAAAAGTTTAATGGTGGCAGCCAGCTGCTCCAAAGCATTTTAGAGGTTAATATAACTGGATTAACCGGGCAAATTAAGTTTAATCCAGATGGAAACTTGATTAATCCTGCATTTGAAGTGATCAATGTGATTGGCACAGGGACTAGGACTATTGGTTATTGGTCCAACTCTTCTGGCCTGTCACTTGATCCACCAGATAAGTCTCAAAGGAAGCTGCACTCTAAGGGTTCCAGCACCGATACTCAACGATTATACAGCATCATCTGGCCGGGACAAACAACTCAGAAGCCTCGTGGGTGGGTTTTCCCTGACAACGGAAGAAAGCTCAGGATTGGAGTCCCCAATCGAGTTAGCTACCACGAGTTTGTAGGCGTAAAAGGCACTGATGAGTTCACTGGCTATTGCATTGAGGTCTTTCAAGCTGCCTTAAACGAGTTACCATATGGTGTCCCATTCCAGTTTGTACCTTTCGGGGATGGTAAAAATAACCCTGAAAACAGTGAACTTCTGCATAGGATCCAAATTGGTGAGTTTGATGGTGTGGTGGGAGATATTACAATCACCACTAGCAGAACAAAGATGGTGGATTTCACACAGCCGTACATTGAGTCTGGTCTAGTTGTGGTTGCTCCAATTAGGAAGACGAACTCTAGTGCTTGGGCATTTTTGAGGCCTTTTACTCCAATGATGTGGGGTATCACAGGCATTTTCTTCCTAGTTGTGGGAGCAGTTGTTTGGATTTTGGAGCGTCGGACTAACGAGGATTTTCGAGGCCATCCGCGGAAACAATTCGTCACAATTATTTGGTTTAGCTTTTCAACTCTGTTCTTCTCTCAAAAAGAAAAAACCGGCAGCACGCTTGGTAGATTTGTGCTTATCATATGGCTATTTGTGGTTCTAATACTCAACTCAAGCTACATTGCTAGCCTGACATCAATCCTTACTGTCGAGCAGCTTTCTTCACCTGTTAAAGGGATTGAAAGTCTGGCAAACAGCAATGATCGCATTGGTTTCCTAAAGAATTCATTCGCTGAAAACTACTTAACTGATGAGCTAAACATACACAAGTCAAGACTTGTGCCTCTTAACTCCCCAGAAGAATATGAGAAGGCCTTGAAAGATGGTCCTAATGCAGGCGGTATTGCTGCAGTGATTGATGAGCGTGCATACATGGAGCTCTTCCTCTCCGTTCGATGTGGATATAGTATCGTAGGTCAAGAATTCACCAAAATGGGCTGGGGTTTTGCCTTTCCAAGGGACTCGCCTCTTGCAATTGACATGTCAACCGCGGTGTTGAAACTATCGGAGAGGGGAGATCTTCAAAAGATTCATGACAAATGGCTGATGAAGAGTGCCTGCAGCGCAGAAGGAGCAAAGCAAGCCGTAGACCGCCTTCCAATGAGGAGCTTTTGGGGCCTCTTTTTGCTGAGCGGCATAGCTTGCTTTCTCGCTCTTGTTCTTCATGTTCTTCGCATGGTGCGCCAGTACTACAGGTTTCATGATTCCGACTGCGACAGCTCACAAGCAAGACGCCTTCGATCCTTCGTTTCGTTTGTAAATAAAAGGGAAGAGGAAGTGAAAAGAAGAACCAAGAGGAGGAGGACTGAGAAGGCTTCAAATAAAGTAGTGCAGGATCAAGATAGTAGTTCCATTGATGGTTTGGATGAATGTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007267 GO:0008066 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009611 GO:0009617 GO:0009682 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009864 GO:0009867 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0031347 GO:0031349 GO:0032870 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0042742 GO:0043200 GO:0043207 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046873 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071395 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0080134 GO:0098542 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

935

Amino Acids

104.33

Weight (kDa)

8.97

Isoelectric Point (pI)

43.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	43 - 347	2.6e-11	Periplasmic binding protein
ANF_receptor	PF01094	60 - 421	1.5e-91	Receptor family ligand binding region
SBP_bac_3	PF00497	494 - 825	1.5e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan-Glu_bd	PF10613	501 - 586	4.2e-09	Ligated ion channel L-glutamate- and glycine-binding site
Lig_chan	PF00060	606 - 857	5.6e-34	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001053)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51480 AT3G51480 AT3G51480 AT3G51480
fragaria_vesca	FvH4_1g10192 FvH4_1g10210 FvH4_1g10210
malus_domestica	MD15G1229300.v1.1 MD15G1229400.v1.1 MD15G1229500.v1.1 MD15G1229600.v1.1
prunus_persica	Prupe.7G184100_v2.0.a1 Prupe.7G184300_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184400_v2.0.a1 Prupe.7G184500_v2.0.a1
pyrus_communis	pycom15g20440 pycom15g20470
rosa_chinensis	RchiOBHm_Chr1g0342071 RchiOBHm_Chr1g0342111 RchiOBHm_Chr1g0342121 RchiOBHm_Chr1g0342221 RchiOBHm_Chr2g0097001 RchiOBHm_Chr2g0097011
rosa_laevigata	RLG00000016693 RLG00000016695 RLG00000016696 RLG00000029615
rosa_multiflora	Rmu_sc0005782.1_g000005 Rmu_sc0006853.1_g000008
rosa_roxburghii	Rroxscaffold_1G00033430 Rroxscaffold_2G00145050 Rroxscaffold_2G00145070 Rroxscaffold_2G00145080 Rroxscaffold_2G00145090 Rroxscaffold_4G00317590 Rroxscaffold_4G00317600
rosa_rugosa	Rorug01G0152800.1 Rorug01G0153000.1 Rorug02G0062800 Rorug02G0063000
rosa_samantha	Rh1AG167500 Rh1AG167800 Rh1BG134100 Rh1BG134200 Rh1CG155400 Rh1DG168300 Rh1DG168400 Rh2AG110600 Rh2BG112400 Rh2BG112500 Rh2CG114500 Rh2CG114600 Rh2DG113700 Rh2DG113800
rosa_wichuraiana	Rw1G013960 Rw2G008560 Rw2G008580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	628
AccB1I	GGYRCC	1 cut(s)	628
AccBSI	CCGCTC	1 cut(s)	2559
AccI	GTMKAC	2 cut(s)	890, 2514
AccII	CGCG	3 cut(s)	140, 1909, 2417
AclWI	GGATC	7 cut(s)	703, 824, 1310, 1639, 1652, 2661, 2769
AcoI	YGGCCR	2 cut(s)	327, 1394
AcsI	RAATTY	5 cut(s)	225, 615, 1084, 2149, 2348
AcyI	GRCGYC	1 cut(s)	2659
AfaI	GTAC	6 cut(s)	94, 623, 630, 1593, 1730, 2615
AfeI	AGCGCT	1 cut(s)	608
AfiI	CCNNNNNNNGG	6 cut(s)	1442, 1849, 2099, 2261, 2382, 2724
AflIII	ACRYGT	2 cut(s)	786, 2406
AhlI	ACTAGT	1 cut(s)	725
Alw21I	GWGCWC	2 cut(s)	747, 2307
Alw26I	GTCTC	2 cut(s)	438, 1335
AlwI	GGATC	7 cut(s)	703, 824, 1310, 1639, 1652, 2661, 2769
Aor51HI	AGCGCT	1 cut(s)	608
AoxI	GGCC	7 cut(s)	327, 1303, 1394, 1799, 1900, 2238, 2542
ApoI	RAATTY	5 cut(s)	225, 615, 1084, 2149, 2348
ArsI	GACNNNNNNTTYG	2 cut(s)	1028, 1060
AseI	ATTAAT	1 cut(s)	1230
Asp700I	GAANNNNTTC	3 cut(s)	1063, 1916, 2153
Asp718I	GGTACC	1 cut(s)	628
AspLEI	GCGC	5 cut(s)	140, 609, 757, 2492, 2610
AspS9I	GGNCC	4 cut(s)	338, 1290, 2252, 2542
AsuC2I	CCSGG	2 cut(s)	1195, 1398
AsuHPI	GGTGA	6 cut(s)	739, 970, 1667, 1681, 2082, 2344
AsuII	TTCGAA	1 cut(s)	460
AsuNHI	GCTAGC	1 cut(s)	2051
AvaII	GGWCC	3 cut(s)	338, 1290, 2252
BalI	TGGCCA	1 cut(s)	329
BamHI	GGATCC	1 cut(s)	1644
BanI	GGYRCC	1 cut(s)	628
BanII	GRGCYC	2 cut(s)	641, 2307
BarI	GAAGNNNNNNTAC	2 cut(s)	2505, 2537
BauI	CACGAG	3 cut(s)	949, 1419, 1487
BbsI	GAAGAC	1 cut(s)	1775
Bbv12I	GWGCWC	2 cut(s)	747, 2307
BccI	CCATC	8 cut(s)	481, 1211, 1598, 1658, 1702, 1911, 2243, 2777
BceAI	ACGGC	2 cut(s)	1711, 2495
BclI	TGATCA	1 cut(s)	1248
BcnI	CCSGG	2 cut(s)	1195, 1398
BcoDI	GTCTC	2 cut(s)	438, 1335
BcuI	ACTAGT	1 cut(s)	725
BfmI	CTRYAG	4 cut(s)	492, 2274, 2485, 2617
BfoI	RGCGCY	1 cut(s)	610
BglII	AGATCT	1 cut(s)	2443
BlpI	GCTNAGC	1 cut(s)	2555
BmcAI	AGTACT	2 cut(s)	94, 2615
Bme1390I	CCNGG	2 cut(s)	1195, 1398
Bme18I	GGWCC	3 cut(s)	338, 1290, 2252
BmgT120I	GGNCC	4 cut(s)	338, 1290, 2252, 2542
BmiI	GGNNCC	5 cut(s)	340, 630, 1357, 1646, 2543
BmrFI	CCNGG	2 cut(s)	1195, 1398
BmsI	GCATC	3 cut(s)	297, 1111, 1395
BmtI	GCTAGC	1 cut(s)	2055
BpiI	GAAGAC	1 cut(s)	1775
Bpu10I	CCTNAGC	1 cut(s)	1454
Bpu1102I	GCTNAGC	1 cut(s)	2555
Bpu14I	TTCGAA	1 cut(s)	460
BpuEI	CTTGAG	1 cut(s)	2023
BpuMI	CCSGG	2 cut(s)	1195, 1398
BsaAI	YACGTR	3 cut(s)	364, 787, 987
BsaBI	GATNNNNATC	2 cut(s)	707, 828
BsaHI	GRCGYC	1 cut(s)	2659
BsaJI	CCNNGG	4 cut(s)	582, 1907, 2381, 2415
BsaXI	ACNNNNNCTCC	4 cut(s)	418, 448, 1845, 1875
Bsc4I	CCNNNNNNNGG	6 cut(s)	1442, 1849, 2099, 2261, 2382, 2724
Bse118I	RCCGGY	1 cut(s)	1976
Bse1I	ACTGG	6 cut(s)	273, 949, 1189, 1528, 1585, 2611
Bse3DI	GCAATG	3 cut(s)	573, 2046, 2131
Bse8I	GATNNNNATC	2 cut(s)	707, 828
BseDI	CCNNGG	4 cut(s)	582, 1907, 2381, 2415
BseGI	GGATG	3 cut(s)	1609, 1903, 2797
BseJI	GATNNNNATC	2 cut(s)	707, 828
BseLI	CCNNNNNNNGG	6 cut(s)	1442, 1849, 2099, 2261, 2382, 2724
BseMI	GCAATG	3 cut(s)	573, 2046, 2131
BseMII	CTCAG	7 cut(s)	103, 363, 626, 1424, 1468, 2546, 2724
BseNI	ACTGG	6 cut(s)	273, 949, 1189, 1528, 1585, 2611
BseRI	GAGGAG	6 cut(s)	484, 967, 1116, 2545, 2737, 2740
BseYI	CCCAGC	1 cut(s)	2364
BsgI	GTGCAG	3 cut(s)	698, 1328, 2777
Bsh1236I	CGCG	3 cut(s)	140, 1909, 2417
BshFI	GGCC	7 cut(s)	329, 1305, 1396, 1801, 1902, 2240, 2544
BshNI	GGYRCC	1 cut(s)	628
BsiHKAI	GWGCWC	2 cut(s)	747, 2307
BsiSI	CCGG	3 cut(s)	1194, 1397, 1977
BslFI	GGGAC	8 cut(s)	324, 380, 602, 1282, 1413, 1451, 1562, 2399
BslI	CCNNNNNNNGG	6 cut(s)	1442, 1849, 2099, 2261, 2382, 2724
BsmAI	GTCTC	2 cut(s)	438, 1335
BsmFI	GGGAC	8 cut(s)	324, 380, 602, 1282, 1413, 1451, 1562, 2399
BsmI	GAATGC	1 cut(s)	574
BsnI	GGCC	7 cut(s)	329, 1305, 1396, 1801, 1902, 2240, 2544
Bsp119I	TTCGAA	1 cut(s)	460
Bsp1286I	GDGCHC	3 cut(s)	641, 747, 2307
Bsp143I	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
Bsp1720I	GCTNAGC	1 cut(s)	2555
BspANI	GGCC	7 cut(s)	329, 1305, 1396, 1801, 1902, 2240, 2544
BspCNI	CTCAG	7 cut(s)	102, 362, 627, 1423, 1467, 2547, 2725
BspFNI	CGCG	3 cut(s)	140, 1909, 2417
BspHI	TCATGA	2 cut(s)	2458, 2626
BspLI	GGNNCC	5 cut(s)	340, 630, 1357, 1646, 2543
BspMAI	CTGCAG	3 cut(s)	496, 2278, 2489
BspOI	GCTAGC	1 cut(s)	2055
BspPI	GGATC	7 cut(s)	703, 824, 1310, 1639, 1652, 2661, 2769
BspQI	GCTCTTC	2 cut(s)	973, 2312
BspT104I	TTCGAA	1 cut(s)	460
BspT107I	GGYRCC	1 cut(s)	628
BsrBI	CCGCTC	1 cut(s)	2559
BsrDI	GCAATG	3 cut(s)	573, 2046, 2131
BsrFI	RCCGGY	1 cut(s)	1976
BsrI	ACTGG	6 cut(s)	273, 949, 1189, 1528, 1585, 2611
BssAI	RCCGGY	1 cut(s)	1976
BssECI	CCNNGG	4 cut(s)	582, 1907, 2381, 2415
BssMI	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
BssNAI	GTATAC	1 cut(s)	891
BssNI	GRCGYC	1 cut(s)	2659
BssSI	CACGAG	3 cut(s)	949, 1419, 1487
BssT1I	CCWWGG	2 cut(s)	582, 2381
Bst1107I	GTATAC	1 cut(s)	891
Bst2BI	CACGAG	3 cut(s)	949, 1419, 1487
Bst4CI	ACNGT	3 cut(s)	325, 1628, 2074
Bst6I	CTCTTC	5 cut(s)	973, 1303, 2312, 2471, 2691
BstACI	GRCGYC	1 cut(s)	2659
BstAPI	GCANNNNNTGC	1 cut(s)	2568
BstBAI	YACGTR	3 cut(s)	364, 787, 987
BstBI	TTCGAA	1 cut(s)	460
BstC8I	GCNNGC	8 cut(s)	1151, 1985, 2053, 2263, 2292, 2485, 2508, 2569
BstDEI	CTNAG	8 cut(s)	89, 349, 635, 1350, 1410, 1454, 2555, 2733
BstDSI	CCRYGG	2 cut(s)	1907, 2415
BstENI	CCTNNNNNAGG	3 cut(s)	2097, 2259, 2380
BstF5I	GGATG	3 cut(s)	1609, 1903, 2797
BstFNI	CGCG	3 cut(s)	140, 1909, 2417
BstH2I	RGCGCY	1 cut(s)	610
BstHHI	GCGC	5 cut(s)	140, 609, 757, 2492, 2610
BstKTI	GATC	9 cut(s)	711, 832, 1251, 1318, 1647, 2131, 2446, 2669, 2764
BstMAI	GTCTC	2 cut(s)	438, 1335
BstMBI	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
BstNSI	RCATGY	2 cut(s)	865, 2410
BstSCI	CCNGG	2 cut(s)	1193, 1396
BstSFI	CTRYAG	4 cut(s)	492, 2274, 2485, 2617
BstSNI	TACGTA	1 cut(s)	987
BstUI	CGCG	3 cut(s)	140, 1909, 2417
BstV2I	GAAGAC	1 cut(s)	1775
BstX2I	RGATCY	3 cut(s)	829, 1644, 2443
BstXI	CCANNNNNNTGG	2 cut(s)	789, 1818
BstYI	RGATCY	3 cut(s)	829, 1644, 2443
BstZ17I	GTATAC	1 cut(s)	891
BsuRI	GGCC	7 cut(s)	329, 1305, 1396, 1801, 1902, 2240, 2544
BtgI	CCRYGG	2 cut(s)	1907, 2415
BtgZI	GCGATG	1 cut(s)	361
BtsCI	GGATG	3 cut(s)	1609, 1903, 2797
BtsI	GCAGTG	2 cut(s)	108, 2283
BtsIMutI	CAGTG	7 cut(s)	108, 280, 671, 1509, 1521, 1633, 2283
Cac8I	GCNNGC	8 cut(s)	1151, 1985, 2053, 2263, 2292, 2485, 2508, 2569
CciI	TCATGA	2 cut(s)	2458, 2626
CfoI	GCGC	5 cut(s)	140, 609, 757, 2492, 2610
Cfr10I	RCCGGY	1 cut(s)	1976
Cfr13I	GGNCC	4 cut(s)	338, 1290, 2252, 2542
Cfr42I	CCGCGG	2 cut(s)	1910, 2418
CseI	GACGC	2 cut(s)	1865, 2667
Csp6I	GTAC	6 cut(s)	93, 622, 629, 1592, 1729, 2614
CspCI	CAANNNNNGTGG	2 cut(s)	1692, 1727
CviAII	CATG	9 cut(s)	28, 862, 1028, 2299, 2407, 2459, 2591, 2602, 2627
CviQI	GTAC	6 cut(s)	93, 622, 629, 1592, 1729, 2614
DdeI	CTNAG	8 cut(s)	89, 349, 635, 1350, 1410, 1454, 2555, 2733
DpnI	GATC	9 cut(s)	710, 831, 1250, 1317, 1646, 2130, 2445, 2668, 2763
DpnII	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
DraI	TTTAAA	2 cut(s)	922, 1128
EaeI	YGGCCR	2 cut(s)	327, 1394
Eam1104I	CTCTTC	5 cut(s)	973, 1303, 2312, 2471, 2691
EarI	CTCTTC	5 cut(s)	973, 1303, 2312, 2471, 2691
EciI	GGCGGA	1 cut(s)	580
Ecl136II	GAGCTC	1 cut(s)	2305
Eco105I	TACGTA	1 cut(s)	987
Eco130I	CCWWGG	2 cut(s)	582, 2381
Eco147I	AGGCCT	2 cut(s)	1801, 2240
Eco24I	GRGCYC	2 cut(s)	641, 2307
Eco47I	GGWCC	3 cut(s)	338, 1290, 2252
Eco47III	AGCGCT	1 cut(s)	608
Eco53kI	GAGCTC	1 cut(s)	2305
EcoICRI	GAGCTC	1 cut(s)	2305
EcoNI	CCTNNNNNAGG	3 cut(s)	2097, 2259, 2380
EcoO109I	RGGNCCY	1 cut(s)	2542
EcoRI	GAATTC	2 cut(s)	2149, 2348
EcoT14I	CCWWGG	2 cut(s)	582, 2381
EcoT38I	GRGCYC	2 cut(s)	641, 2307
ErhI	CCWWGG	2 cut(s)	582, 2381
FaeI	CATG	9 cut(s)	31, 865, 1031, 2302, 2410, 2462, 2594, 2605, 2630
FaqI	GGGAC	8 cut(s)	324, 380, 602, 1282, 1413, 1451, 1562, 2399
FatI	CATG	9 cut(s)	27, 861, 1027, 2298, 2406, 2458, 2590, 2601, 2626
FauNDI	CATATG	2 cut(s)	1570, 2009
FbaI	TGATCA	1 cut(s)	1248
FblI	GTMKAC	2 cut(s)	890, 2514
FokI	GGATG	2 cut(s)	1616, 1890
FriOI	GRGCYC	2 cut(s)	641, 2307
GlaI	GCGC	5 cut(s)	139, 608, 756, 2491, 2609
GsaI	CCCAGC	1 cut(s)	2368
HaeII	RGCGCY	1 cut(s)	610
HaeIII	GGCC	7 cut(s)	329, 1305, 1396, 1801, 1902, 2240, 2544
HapII	CCGG	3 cut(s)	1194, 1397, 1977
HgaI	GACGC	2 cut(s)	1865, 2667
HhaI	GCGC	5 cut(s)	140, 609, 757, 2492, 2610
Hin1I	GRCGYC	1 cut(s)	2659
Hin1II	CATG	9 cut(s)	31, 865, 1031, 2302, 2410, 2462, 2594, 2605, 2630
Hin6I	GCGC	5 cut(s)	138, 607, 755, 2490, 2608
HinP1I	GCGC	5 cut(s)	138, 607, 755, 2490, 2608
HincII	GTYRAC	1 cut(s)	2412
HindII	GTYRAC	1 cut(s)	2412
HindIII	AAGCTT	1 cut(s)	297
HpaII	CCGG	3 cut(s)	1194, 1397, 1977
HphI	GGTGA	6 cut(s)	739, 970, 1667, 1681, 2082, 2344
Hpy166II	GTNNAC	9 cut(s)	86, 130, 309, 737, 891, 1521, 1631, 2412, 2515
Hpy188I	TCNGA	8 cut(s)	486, 636, 829, 904, 1413, 1881, 2434, 2635
Hpy188III	TCNNGA	9 cut(s)	221, 664, 1214, 1456, 2199, 2345, 2459, 2627, 2765
Hpy8I	GTNNAC	9 cut(s)	86, 130, 309, 737, 891, 1521, 1631, 2412, 2515
Hpy99I	CGWCG	1 cut(s)	1881
HpyAV	CCTTC	9 cut(s)	661, 843, 852, 2230, 2489, 2531, 2672, 2680, 2731
HpyCH4III	ACNGT	3 cut(s)	325, 1628, 2074
HpyCH4IV	ACGT	3 cut(s)	363, 786, 986
HpyF3I	CTNAG	8 cut(s)	89, 349, 635, 1350, 1410, 1454, 2555, 2733
HpySE526I	ACGT	3 cut(s)	363, 786, 986
Hsp92I	GRCGYC	1 cut(s)	2659
Hsp92II	CATG	9 cut(s)	31, 865, 1031, 2302, 2410, 2462, 2594, 2605, 2630
HspAI	GCGC	5 cut(s)	138, 607, 755, 2490, 2608
KpnI	GGTACC	1 cut(s)	632
Ksp22I	TGATCA	1 cut(s)	1248
KspI	CCGCGG	2 cut(s)	1910, 2418
Kzo9I	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
LguI	GCTCTTC	2 cut(s)	973, 2312
LmnI	GCTCC	8 cut(s)	933, 1161, 1762, 1853, 1873, 2302, 2498, 2532
LweI	GCATC	3 cut(s)	297, 1111, 1395
MaeII	ACGT	3 cut(s)	363, 786, 986
MaeIII	GTNAC	6 cut(s)	106, 275, 473, 1308, 1563, 1921
MalI	GATC	9 cut(s)	710, 831, 1250, 1317, 1646, 2130, 2445, 2668, 2763
MbiI	CCGCTC	1 cut(s)	2559
MboI	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
MfeI	CAATTG	1 cut(s)	2400
MflI	RGATCY	3 cut(s)	829, 1644, 2443
MhlI	GDGCHC	3 cut(s)	641, 747, 2307
MlsI	TGGCCA	1 cut(s)	329
MluNI	TGGCCA	1 cut(s)	329
MlyI	GAGTC	8 cut(s)	81, 704, 787, 1031, 1073, 1473, 1745, 2381
MmeI	TCCRAC	3 cut(s)	1317, 1859, 2658
Mox20I	TGGCCA	1 cut(s)	329
MroXI	GAANNNNTTC	3 cut(s)	1063, 1916, 2153
MscI	TGGCCA	1 cut(s)	329
MslI	CAYNNNNRTG	2 cut(s)	787, 2357
Msp20I	TGGCCA	1 cut(s)	329
MspA1I	CMGCKG	3 cut(s)	1153, 1909, 2417
MspI	CCGG	3 cut(s)	1194, 1397, 1977
MspR9I	CCNGG	2 cut(s)	1195, 1398
MunI	CAATTG	1 cut(s)	2400
Mva1269I	GAATGC	1 cut(s)	574
MvnI	CGCG	3 cut(s)	140, 1909, 2417
NciI	CCSGG	2 cut(s)	1195, 1398
NdeI	CATATG	2 cut(s)	1570, 2009
NdeII	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
NheI	GCTAGC	1 cut(s)	2051
NlaIII	CATG	9 cut(s)	31, 865, 1031, 2302, 2410, 2462, 2594, 2605, 2630
NlaIV	GGNNCC	5 cut(s)	340, 630, 1357, 1646, 2543
NmuCI	GTSAC	5 cut(s)	106, 275, 473, 1308, 1921
NspI	RCATGY	2 cut(s)	865, 2410
NspV	TTCGAA	1 cut(s)	460
PagI	TCATGA	2 cut(s)	2458, 2626
PceI	AGGCCT	2 cut(s)	1801, 2240
PciI	ACATGT	1 cut(s)	2406
PciSI	GCTCTTC	2 cut(s)	973, 2312
PctI	GAATGC	1 cut(s)	574
PdmI	GAANNNNTTC	3 cut(s)	1063, 1916, 2153
PfeI	GAWTC	7 cut(s)	31, 155, 824, 899, 1007, 2455, 2630
PleI	GAGTC	8 cut(s)	81, 703, 786, 1031, 1073, 1472, 1744, 2381
PpsI	GAGTC	8 cut(s)	81, 703, 786, 1031, 1073, 1472, 1744, 2381
Ppu21I	YACGTR	3 cut(s)	364, 787, 987
PscI	ACATGT	1 cut(s)	2406
PshBI	ATTAAT	1 cut(s)	1230
Psp124BI	GAGCTC	1 cut(s)	2307
PspFI	CCCAGC	1 cut(s)	2364
PspN4I	GGNNCC	5 cut(s)	340, 630, 1357, 1646, 2543
PspPI	GGNCC	4 cut(s)	338, 1290, 2252, 2542
PstI	CTGCAG	3 cut(s)	496, 2278, 2489
PsuI	RGATCY	3 cut(s)	829, 1644, 2443
PvuII	CAGCTG	1 cut(s)	1153
RsaI	GTAC	6 cut(s)	94, 623, 630, 1593, 1730, 2615
RsaNI	GTAC	6 cut(s)	93, 622, 629, 1592, 1729, 2614
RseI	CAYNNNNRTG	2 cut(s)	787, 2357
SacI	GAGCTC	1 cut(s)	2307
SacII	CCGCGG	2 cut(s)	1910, 2418
SapI	GCTCTTC	2 cut(s)	973, 2312
Sau3AI	GATC	9 cut(s)	708, 829, 1248, 1315, 1644, 2128, 2443, 2666, 2761
Sau96I	GGNCC	4 cut(s)	338, 1290, 2252, 2542
ScaI	AGTACT	2 cut(s)	94, 2615
SchI	GAGTC	8 cut(s)	81, 704, 787, 1031, 1073, 1473, 1745, 2381
ScrFI	CCNGG	2 cut(s)	1195, 1398
SduI	GDGCHC	3 cut(s)	641, 747, 2307
SfaNI	GCATC	3 cut(s)	297, 1111, 1395
SfcI	CTRYAG	4 cut(s)	492, 2274, 2485, 2617
Sfr303I	CCGCGG	2 cut(s)	1910, 2418
SfuI	TTCGAA	1 cut(s)	460
SgrBI	CCGCGG	2 cut(s)	1910, 2418
SinI	GGWCC	3 cut(s)	338, 1290, 2252
SmiMI	CAYNNNNRTG	2 cut(s)	787, 2357
SmlI	CTYRAG	1 cut(s)	2038
SmoI	CTYRAG	1 cut(s)	2038
SnaBI	TACGTA	1 cut(s)	987
SpeI	ACTAGT	1 cut(s)	725
SseBI	AGGCCT	2 cut(s)	1801, 2240
SspI	AATATT	1 cut(s)	1063
SstI	GAGCTC	1 cut(s)	2307
StuI	AGGCCT	2 cut(s)	1801, 2240
StyD4I	CCNGG	2 cut(s)	1193, 1396
StyI	CCWWGG	2 cut(s)	582, 2381
TaaI	ACNGT	3 cut(s)	325, 1628, 2074
TaiI	ACGT	3 cut(s)	366, 789, 989
TaqI	TCGA	8 cut(s)	153, 460, 776, 1474, 1897, 2076, 2320, 2665
TatI	WGTACW	2 cut(s)	92, 2613
TauI	GCSGC	2 cut(s)	416, 2562
TfiI	GAWTC	7 cut(s)	31, 155, 824, 899, 1007, 2455, 2630
TscAI	CASTG	7 cut(s)	115, 280, 678, 1516, 1528, 1633, 2283
TseFI	GTSAC	5 cut(s)	106, 275, 473, 1308, 1921
Tsp45I	GTSAC	5 cut(s)	106, 275, 473, 1308, 1921
TspDTI	ATGAA	7 cut(s)	44, 672, 2142, 2447, 2489, 2579, 2615
TspGWI	ACGGA	4 cut(s)	326, 589, 1458, 2305
TspRI	CASTG	7 cut(s)	115, 280, 678, 1516, 1528, 1633, 2283
VpaK11BI	GGWCC	3 cut(s)	338, 1290, 2252
VspI	ATTAAT	1 cut(s)	1230
XagI	CCTNNNNNAGG	3 cut(s)	2097, 2259, 2380
XapI	RAATTY	5 cut(s)	225, 615, 1084, 2149, 2348
XceI	RCATGY	2 cut(s)	865, 2410
XcmI	CCANNNNNNNNNTGG	2 cut(s)	580, 2785
XmiI	GTMKAC	2 cut(s)	890, 2514
XmnI	GAANNNNTTC	3 cut(s)	1063, 1916, 2153
ZrmI	AGTACT	2 cut(s)	94, 2615

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.