Prupe.5G177900_v2.0.a1

Pollen-specific protein SF3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000896
Physical Location & Seq
Reverse (-)
15031754 .. 15034574
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G177900.1

Sequence Viewer

Length: 564 bp
ATGGCAACATTTGCAGGGACAACCCAGAAGTGCAAAGCATGTGAGAAGACTGTCTACTTGGTTGATCAGCTCACTGCTGACAACAAAGTCTACCACAAGGCATGCTTTAGATGCCACCACTGCAAGGGCACCCTCAAGCTTAGTAATTATTCCTCTTTTGAGGGTGTTTTGTATTGCAAGCCTCACTTTGATCAGCTATTTAAGATGACTGGAAGCTTGGATAAAAGTTTTGAAGGTATACCAAAAACTGTTAGAGACAGATCAGAACAGGTCAACAACAGTAAAGTTTCTAGTTTATTTGCCGGGACTCAAGACAAGTGTGTTGCTTGCAAGAAAACAGTTTACCCCATTGAAAAGGTGGCAGTTGATGGCACATCATACCATAAGCCTTGTTTCAGGTGCAGCCATGGAGGCTGTGTGATCAGCCCATCAAACTACGTAGCTCATGAGCATCGTCTCTACTGCAGGCATCACCATAGCCAACTCTTCAAGGAGAAAGGAAACTTCAGCCAGCTTAGCAAACAGGAAGAAGTTCAAGGGGTGACTGAGAACGCAGAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIM

Protein Analysis

188

Amino Acids

21.05

Weight (kDa)

8.87

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AccB1I GGYRCC 1 cut(s) 128
AccI GTMKAC 3 cut(s) 54, 90, 238
AcuI CTGAAG 1 cut(s) 490
AfiI CCNNNNNNNGG 1 cut(s) 124
AgsI TTSAA 4 cut(s) 233, 353, 490, 536
AluBI AGCT 6 cut(s) 70, 139, 196, 216, 443, 514
AluI AGCT 6 cut(s) 70, 139, 196, 216, 443, 514
Alw26I GTCTC 2 cut(s) 249, 461
ApeKI GCWGC 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 531
AsuC2I CCSGG 1 cut(s) 304
AsuHPI GGTGA 2 cut(s) 464, 553
BaeGI GKGCMC 1 cut(s) 131
BanI GGYRCC 1 cut(s) 128
BarI GAAGNNNNNNTAC 2 cut(s) 38, 70
BbsI GAAGAC 1 cut(s) 53
BbvI GCAGC 1 cut(s) 414
BccI CCATC 2 cut(s) 362, 436
BclI TGATCA 3 cut(s) 64, 190, 420
BcnI CCSGG 1 cut(s) 304
BcoDI GTCTC 2 cut(s) 249, 461
BfaI CTAG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 463
BglI GCCNNNNNGGC 1 cut(s) 411
BisI GCNGC 1 cut(s) 403
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 1 cut(s) 404
Bme1390I CCNGG 1 cut(s) 304
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 101, 460, 478
BpiI GAAGAC 1 cut(s) 53
Bpu1102I GCTNAGC 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 119, 294
BpuMI CCSGG 1 cut(s) 304
BsaAI YACGTR 1 cut(s) 439
BsaJI CCNNGG 1 cut(s) 406
BsaXI ACNNNNNCTCC 2 cut(s) 402, 432
Bsc4I CCNNNNNNNGG 1 cut(s) 124
Bse1I ACTGG 1 cut(s) 214
BseDI CCNNGG 1 cut(s) 406
BseLI CCNNNNNNNGG 1 cut(s) 124
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 1 cut(s) 214
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 1 cut(s) 414
BsgI GTGCAG 1 cut(s) 421
BshNI GGYRCC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 303
BslFI GGGAC 2 cut(s) 31, 319
BslI CCNNNNNNNGG 1 cut(s) 124
BsmAI GTCTC 2 cut(s) 249, 461
BsmBI CGTCTC 1 cut(s) 461
BsmFI GGGAC 2 cut(s) 31, 319
Bsp1286I GDGCHC 1 cut(s) 131
Bsp143I GATC 4 cut(s) 64, 190, 260, 420
Bsp1720I GCTNAGC 1 cut(s) 515
Bsp19I CCATGG 1 cut(s) 406
BspCNI CTCAG 1 cut(s) 538
BspHI TCATGA 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 128
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 1 cut(s) 406
BssMI GATC 4 cut(s) 64, 190, 260, 420
BssNAI GTATAC 1 cut(s) 239
BssT1I CCWWGG 1 cut(s) 406
Bst1107I GTATAC 1 cut(s) 239
Bst4CI ACNGT 4 cut(s) 52, 250, 281, 340
Bst6I CTCTTC 1 cut(s) 491
BstAPI GCANNNNNTGC 1 cut(s) 11
BstBAI YACGTR 1 cut(s) 439
BstC8I GCNNGC 5 cut(s) 103, 179, 328, 467, 512
BstDEI CTNAG 4 cut(s) 140, 515, 546, 561
BstDSI CCRYGG 1 cut(s) 406
BstKTI GATC 4 cut(s) 67, 193, 263, 423
BstMAI GTCTC 2 cut(s) 249, 461
BstMBI GATC 4 cut(s) 64, 190, 260, 420
BstMWI GCNNNNNNNGC 5 cut(s) 11, 111, 120, 411, 516
BstNSI RCATGY 2 cut(s) 42, 105
BstSCI CCNGG 1 cut(s) 302
BstSFI CTRYAG 1 cut(s) 463
BstSLI GKGCMC 1 cut(s) 131
BstSNI TACGTA 1 cut(s) 439
BstV1I GCAGC 1 cut(s) 414
BstV2I GAAGAC 1 cut(s) 53
BstZ17I GTATAC 1 cut(s) 239
BtgI CCRYGG 1 cut(s) 406
BtsI GCAGTG 2 cut(s) 72, 118
BtsIMutI CAGTG 2 cut(s) 72, 118
Cac8I GCNNGC 5 cut(s) 103, 179, 328, 467, 512
CciI TCATGA 1 cut(s) 445
CviAII CATG 4 cut(s) 39, 102, 407, 446
DdeI CTNAG 4 cut(s) 140, 515, 546, 561
DpnI GATC 4 cut(s) 66, 192, 262, 422
DpnII GATC 4 cut(s) 64, 190, 260, 420
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
Eam1104I CTCTTC 1 cut(s) 491
EarI CTCTTC 1 cut(s) 491
Eco105I TACGTA 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 406
Eco57I CTGAAG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 406
Esp3I CGTCTC 1 cut(s) 461
FaeI CATG 4 cut(s) 42, 105, 410, 449
FaiI YATR 8 cut(s) 40, 103, 239, 379, 384, 408, 447, 477
FalI AAGNNNNNCTT 4 cut(s) 89, 121, 170, 202
FaqI GGGAC 2 cut(s) 31, 319
FatI CATG 4 cut(s) 38, 101, 406, 445
FbaI TGATCA 3 cut(s) 64, 190, 420
FblI GTMKAC 3 cut(s) 54, 90, 238
Fnu4HI GCNGC 1 cut(s) 403
Fsp4HI GCNGC 1 cut(s) 403
FspBI CTAG 1 cut(s) 291
GluI GCNGC 1 cut(s) 403
HapII CCGG 1 cut(s) 303
Hin1II CATG 4 cut(s) 42, 105, 410, 449
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 2 cut(s) 137, 214
HinfI GANTC 1 cut(s) 307
HpaII CCGG 1 cut(s) 303
HphI GGTGA 2 cut(s) 464, 553
Hpy166II GTNNAC 5 cut(s) 55, 91, 239, 274, 343
Hpy188I TCNGA 1 cut(s) 265
Hpy188III TCNNGA 2 cut(s) 311, 446
Hpy8I GTNNAC 5 cut(s) 55, 91, 239, 274, 343
HpyAV CCTTC 1 cut(s) 227
HpyCH4III ACNGT 4 cut(s) 52, 250, 281, 340
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 7 cut(s) 14, 33, 123, 177, 330, 402, 465
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 111, 120, 411, 516
HpyF3I CTNAG 4 cut(s) 140, 515, 546, 561
HpySE526I ACGT 1 cut(s) 438
Hsp92II CATG 4 cut(s) 42, 105, 410, 449
Ksp22I TGATCA 3 cut(s) 64, 190, 420
Kzo9I GATC 4 cut(s) 64, 190, 260, 420
LpnPI CCDG 8 cut(s) 38, 195, 254, 316, 382, 451, 509, 524
Lsp1109I GCAGC 1 cut(s) 414
LweI GCATC 3 cut(s) 101, 460, 478
MaeI CTAG 1 cut(s) 291
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 1 cut(s) 541
MalI GATC 4 cut(s) 66, 192, 262, 422
MboI GATC 4 cut(s) 64, 190, 260, 420
MboII GAAGA 3 cut(s) 58, 478, 539
MhlI GDGCHC 1 cut(s) 131
MluCI AATT 1 cut(s) 145
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 6 cut(s) 143, 154, 163, 192, 404, 550
MroXI GAANNNNTTC 1 cut(s) 531
MseI TTAA 1 cut(s) 201
MspI CCGG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 304
MwoI GCNNNNNNNGC 5 cut(s) 11, 111, 120, 411, 516
NciI CCSGG 1 cut(s) 304
NcoI CCATGG 1 cut(s) 406
NdeII GATC 4 cut(s) 64, 190, 260, 420
NlaIII CATG 4 cut(s) 42, 105, 410, 449
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 541
NspI RCATGY 2 cut(s) 42, 105
PaeI GCATGC 1 cut(s) 105
PagI TCATGA 1 cut(s) 445
PdmI GAANNNNTTC 1 cut(s) 531
PkrI GCNGC 1 cut(s) 404
PleI GAGTC 1 cut(s) 301
PpsI GAGTC 1 cut(s) 301
Ppu21I YACGTR 1 cut(s) 439
PspN4I GGNNCC 1 cut(s) 130
PstI CTGCAG 1 cut(s) 467
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 1 cut(s) 403
Sau3AI GATC 4 cut(s) 64, 190, 260, 420
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 1 cut(s) 304
SduI GDGCHC 1 cut(s) 131
SfaNI GCATC 3 cut(s) 101, 460, 478
SfcI CTRYAG 1 cut(s) 463
SmlI CTYRAG 2 cut(s) 134, 309
SmoI CTYRAG 2 cut(s) 134, 309
SnaBI TACGTA 1 cut(s) 439
SphI GCATGC 1 cut(s) 105
Sse9I AATT 1 cut(s) 145
SspMI CTAG 1 cut(s) 291
StyD4I CCNGG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 4 cut(s) 52, 250, 281, 340
TaiI ACGT 1 cut(s) 441
TasI AATT 1 cut(s) 145
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 2 cut(s) 79, 125
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 402
Tsp45I GTSAC 1 cut(s) 541
TspRI CASTG 2 cut(s) 79, 125
XceI RCATGY 2 cut(s) 42, 105
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XmiI GTMKAC 3 cut(s) 54, 90, 238
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.