Rw6G004940

Zinc-binding domain present in Lin-11, Isl-1, Mec-3.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000896
Physical Location & Seq
Reverse (-)
8121210 .. 8123614
2405 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G004940.1

Sequence Viewer

Length: 564 bp
ATGGCCACCTTCGCAGGAACCTCCCAGAAGTGCACAGCCTGCGAAAAGACGGTGTATCTGGTTGACAAGTTAACAGCTGATTCCAGATCCTACCACAAAGCCTGCTTCCGATGCCACCACTGCAGAGGTACCCTAAAGCTCAGCAACTTCTGCTCATTTGAGGGAGTCCTATACTGCAGGCCTCACTATGATCAACTCTTCAAGCGAACTGGTAGTCTGGATAAGAGTTTTGAAGGCACACCAAAAATTTTGAAACCAGAAAAACCTATGATTGAGAATGAGAATAACAAGGCAGTATCAAACATGTTTGTTGGTACCAAAGATAAATGTGTGGGATGTGAAAAGACGGTGTATCCCATTGAGAAGGTCTCGGTGAATGGGACTTCATACCACAGGAGGTGCTTCAAATGTACCCATGGAGGTTGCACCATAAGCCCATCTAACTATATTGCACATGAAGGAAAGCTCTACTGCAAACACCACCACATCCAACTCTTCAAGGAGAAAGGGAATTACAGCCAGCTTGAGAATGAACGTGAAAAGCAATCAGAGACTGGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIM

Protein Analysis

187

Amino Acids

21.27

Weight (kDa)

8.98

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIM PF00412 11 - 66 1.4e-09 LIM domain
LIM PF00412 110 - 166 7.3e-12 LIM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 128, 314
AccB1I GGYRCC 2 cut(s) 128, 314
AclWI GGATC 1 cut(s) 81
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 246
AfaI GTAC 3 cut(s) 130, 316, 412
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 5 cut(s) 202, 233, 253, 406, 499
AluBI AGCT 4 cut(s) 77, 139, 466, 523
AluI AGCT 4 cut(s) 77, 139, 466, 523
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 2 cut(s) 373, 545
Alw44I GTGCAC 1 cut(s) 31
AlwI GGATC 1 cut(s) 81
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 2 cut(s) 3, 179
ApaLI GTGCAC 1 cut(s) 31
ApoI RAATTY 1 cut(s) 246
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
Asp718I GGTACC 2 cut(s) 128, 314
AsuHPI GGTGA 1 cut(s) 385
BaeGI GKGCMC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 128, 314
Bbv12I GWGCWC 1 cut(s) 35
BccI CCATC 1 cut(s) 445
BciVI GTATCC 1 cut(s) 363
BclI TGATCA 1 cut(s) 190
BcoDI GTCTC 2 cut(s) 373, 545
BfmI CTRYAG 2 cut(s) 121, 175
BfuI GTATCC 1 cut(s) 363
BlpI GCTNAGC 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 19, 130, 316
BmsI GCATC 1 cut(s) 101
BplI GAGNNNNNCTC 2 cut(s) 353, 385
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 545
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 1 cut(s) 415
Bse1I ACTGG 2 cut(s) 214, 559
BseDI CCNNGG 1 cut(s) 415
BseGI GGATG 2 cut(s) 341, 486
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 2 cut(s) 214, 559
BseSI GKGCMC 1 cut(s) 35
BshFI GGCC 2 cut(s) 5, 181
BshNI GGYRCC 2 cut(s) 128, 314
BsiHKAI GWGCWC 1 cut(s) 35
BslFI GGGAC 1 cut(s) 394
BsmAI GTCTC 2 cut(s) 373, 545
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 2 cut(s) 5, 181
Bso31I GGTCTC 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 2 cut(s) 86, 190
Bsp1720I GCTNAGC 1 cut(s) 140
Bsp19I CCATGG 1 cut(s) 415
BspANI GGCC 2 cut(s) 5, 181
BspCNI CTCAG 1 cut(s) 153
BspLI GGNNCC 3 cut(s) 19, 130, 316
BspMAI CTGCAG 2 cut(s) 125, 179
BspPI GGATC 1 cut(s) 81
BspT107I GGYRCC 2 cut(s) 128, 314
BspTNI GGTCTC 1 cut(s) 373
BsrI ACTGG 2 cut(s) 214, 559
BssECI CCNNGG 1 cut(s) 415
BssMI GATC 2 cut(s) 86, 190
BssT1I CCWWGG 1 cut(s) 415
Bst4CI ACNGT 2 cut(s) 52, 349
Bst6I CTCTTC 2 cut(s) 203, 500
BstAPI GCANNNNNTGC 2 cut(s) 39, 150
BstC8I GCNNGC 4 cut(s) 40, 103, 179, 521
BstDEI CTNAG 1 cut(s) 140
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 2 cut(s) 341, 486
BstKTI GATC 2 cut(s) 89, 193
BstMAI GTCTC 2 cut(s) 373, 545
BstMBI GATC 2 cut(s) 86, 190
BstMWI GCNNNNNNNGC 6 cut(s) 11, 39, 111, 120, 150, 432
BstNSI RCATGY 1 cut(s) 307
BstSFI CTRYAG 2 cut(s) 121, 175
BstSLI GKGCMC 1 cut(s) 35
BstX2I RGATCY 1 cut(s) 86
BstYI RGATCY 1 cut(s) 86
BsuI GTATCC 1 cut(s) 363
BsuRI GGCC 2 cut(s) 5, 181
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 2 cut(s) 341, 486
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 118
Cac8I GCNNGC 4 cut(s) 40, 103, 179, 521
CaiI CAGNNNCTG 1 cut(s) 554
Csp6I GTAC 3 cut(s) 129, 315, 411
CviAII CATG 3 cut(s) 304, 416, 455
CviQI GTAC 3 cut(s) 129, 315, 411
DdeI CTNAG 1 cut(s) 140
DpnI GATC 2 cut(s) 88, 192
DpnII GATC 2 cut(s) 86, 190
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 203, 500
EarI CTCTTC 2 cut(s) 203, 500
Eco130I CCWWGG 1 cut(s) 415
Eco147I AGGCCT 1 cut(s) 181
Eco31I GGTCTC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 3 cut(s) 307, 419, 458
FaiI YATR 9 cut(s) 172, 189, 269, 305, 388, 417, 431, 447, 456
FaqI GGGAC 1 cut(s) 394
FatI CATG 3 cut(s) 303, 415, 454
FbaI TGATCA 1 cut(s) 190
FokI GGATG 2 cut(s) 348, 473
HaeIII GGCC 2 cut(s) 5, 181
Hin1II CATG 3 cut(s) 307, 419, 458
HincII GTYRAC 2 cut(s) 64, 72
HindII GTYRAC 2 cut(s) 64, 72
HinfI GANTC 2 cut(s) 80, 165
HpaI GTTAAC 1 cut(s) 72
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 3 cut(s) 33, 64, 72
Hpy188I TCNGA 2 cut(s) 110, 550
Hpy188III TCNNGA 2 cut(s) 84, 218
Hpy8I GTNNAC 3 cut(s) 33, 64, 72
HpyAV CCTTC 4 cut(s) 19, 227, 358, 452
HpyCH4III ACNGT 2 cut(s) 52, 349
HpyCH4IV ACGT 1 cut(s) 535
HpyCH4V TGCA 6 cut(s) 33, 123, 177, 426, 452, 474
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 39, 111, 120, 150, 432
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 535
Hsp92II CATG 3 cut(s) 307, 419, 458
KpnI GGTACC 2 cut(s) 132, 318
Ksp22I TGATCA 1 cut(s) 190
KspAI GTTAAC 1 cut(s) 72
Kzo9I GATC 2 cut(s) 86, 190
LweI GCATC 1 cut(s) 101
MaeII ACGT 1 cut(s) 535
MalI GATC 2 cut(s) 88, 192
MboI GATC 2 cut(s) 86, 190
MboII GAAGA 2 cut(s) 190, 487
MflI RGATCY 1 cut(s) 86
MhlI GDGCHC 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 246, 511
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 514
MnlI CCTC 6 cut(s) 31, 119, 154, 192, 390, 413
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 71
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 77
MwoI GCNNNNNNNGC 6 cut(s) 11, 39, 111, 120, 150, 432
NcoI CCATGG 1 cut(s) 415
NdeII GATC 2 cut(s) 86, 190
NlaIII CATG 3 cut(s) 307, 419, 458
NlaIV GGNNCC 3 cut(s) 19, 130, 316
NspI RCATGY 1 cut(s) 307
PceI AGGCCT 1 cut(s) 181
PciI ACATGT 1 cut(s) 303
PfeI GAWTC 1 cut(s) 80
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PscI ACATGT 1 cut(s) 303
PspN4I GGNNCC 3 cut(s) 19, 130, 316
PstI CTGCAG 2 cut(s) 125, 179
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 1 cut(s) 86
PvuII CAGCTG 1 cut(s) 77
RsaI GTAC 3 cut(s) 130, 316, 412
RsaNI GTAC 3 cut(s) 129, 315, 411
SaqAI TTAA 1 cut(s) 71
Sau3AI GATC 2 cut(s) 86, 190
SchI GAGTC 1 cut(s) 174
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 1 cut(s) 101
SfcI CTRYAG 2 cut(s) 121, 175
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 2 cut(s) 246, 511
SseBI AGGCCT 1 cut(s) 181
StuI AGGCCT 1 cut(s) 181
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 2 cut(s) 52, 349
TaiI ACGT 1 cut(s) 538
TasI AATT 2 cut(s) 246, 511
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 1 cut(s) 71
Tru9I TTAA 1 cut(s) 71
TscAI CASTG 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 375, 471, 546
TspRI CASTG 1 cut(s) 125
VneI GTGCAC 1 cut(s) 31
XapI RAATTY 1 cut(s) 246
XceI RCATGY 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.