Rroxscaffold_155G00443910

Pollen-specific protein SF3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000155
OG0000896
Physical Location & Seq
Forward (+)
78194 .. 80848
2655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_155G00443910.1

Sequence Viewer

Length: 567 bp
ATGGCAACATTTGCAGGGACAACCCAGAAGTGCAAGGCATGTGAGAAGACTGTGTACTTGGTTGATCAGCTCACTGCTGACAACAAAGTCTACCACAAGGCTTGTTTCAGATGCCACCACTGCAAAGGAACCCTCAAGCTTAGTAATTATTCGTCGTTCGAGGGTGTTTTATATTGCAAGCCTCACTTTGATCAGCTATTTAAAATGACTGGCAGCTTGGATAAAAGTTTTGAAGGTAACCCAAAAACTGTGAGAGACAGGTCTGCTGATCAGGTCAATAGCAAAGTTTCCAGTATGTTTGCTGGAACTCAAGAAAAGTGTGTTGCTTGCAAGAAAACAGTTTACCCAACTGAAAAGGTGGGAGTTGATGGAACATCATACCATAAGGCTTGTTTCAGGTGCACTCATGGCGGCTGTGTGATCAGTCCATCAAACTATGTAGCTCACGAGCACCGTCTCTATTGTAAGCATCACCATAACCAGCTATTCAAGCAGAAGGGAAACTTCAGCCAACTTGACAAGCAGGAAGAAGCTAAAGAGGTGACTGAGAACACAGTGGCGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIM

Protein Analysis

188

Amino Acids

21.19

Weight (kDa)

8.86

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIM PF00412 11 - 66 4.5e-11 LIM domain
LIM PF00412 107 - 163 1.6e-10 LIM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AccI GTMKAC 1 cut(s) 90
AciI CCGC 2 cut(s) 411, 560
AcuI CTGAAG 1 cut(s) 490
AfaI GTAC 1 cut(s) 56
AgsI TTSAA 2 cut(s) 233, 490
AluBI AGCT 7 cut(s) 70, 139, 196, 216, 443, 484, 533
AluI AGCT 7 cut(s) 70, 139, 196, 216, 443, 484, 533
Alw21I GWGCWC 2 cut(s) 404, 453
Alw26I GTCTC 2 cut(s) 249, 461
Alw44I GTGCAC 1 cut(s) 400
ApaLI GTGCAC 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 213
AsuHPI GGTGA 2 cut(s) 464, 553
BaeGI GKGCMC 1 cut(s) 404
BarI GAAGNNNNNNTAC 2 cut(s) 38, 70
BauI CACGAG 1 cut(s) 446
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 404, 453
BbvI GCAGC 1 cut(s) 225
BccI CCATC 2 cut(s) 362, 436
BclI TGATCA 4 cut(s) 64, 190, 268, 420
BcoDI GTCTC 2 cut(s) 249, 461
BisI GCNGC 2 cut(s) 214, 412
BlsI GCNGC 2 cut(s) 215, 413
BmiI GGNNCC 1 cut(s) 130
BmsI GCATC 2 cut(s) 101, 478
BpiI GAAGAC 1 cut(s) 53
BpuEI CTTGAG 2 cut(s) 119, 294
Bse1I ACTGG 2 cut(s) 214, 291
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 2 cut(s) 214, 291
BseSI GKGCMC 1 cut(s) 404
BseXI GCAGC 1 cut(s) 225
BsiHKAI GWGCWC 2 cut(s) 404, 453
BslFI GGGAC 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 249, 461
BsmBI CGTCTC 1 cut(s) 461
BsmFI GGGAC 1 cut(s) 31
Bsp1286I GDGCHC 2 cut(s) 404, 453
Bsp143I GATC 4 cut(s) 64, 190, 268, 420
BspACI CCGC 2 cut(s) 411, 560
BspCNI CTCAG 1 cut(s) 538
BspLI GGNNCC 1 cut(s) 130
BsrI ACTGG 2 cut(s) 214, 291
BssMI GATC 4 cut(s) 64, 190, 268, 420
BssSI CACGAG 1 cut(s) 446
Bst2BI CACGAG 1 cut(s) 446
Bst4CI ACNGT 5 cut(s) 52, 250, 340, 455, 556
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 179, 328
BstDEI CTNAG 2 cut(s) 140, 546
BstEII GGTNACC 1 cut(s) 236
BstKTI GATC 4 cut(s) 67, 193, 271, 423
BstMAI GTCTC 2 cut(s) 249, 461
BstMBI GATC 4 cut(s) 64, 190, 268, 420
BstMWI GCNNNNNNNGC 4 cut(s) 11, 120, 408, 490
BstNSI RCATGY 1 cut(s) 42
BstPI GGTNACC 1 cut(s) 236
BstSLI GKGCMC 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 225
BstV2I GAAGAC 1 cut(s) 53
BtsI GCAGTG 2 cut(s) 72, 118
BtsIMutI CAGTG 3 cut(s) 72, 118, 561
Cac8I GCNNGC 2 cut(s) 179, 328
Csp6I GTAC 1 cut(s) 55
CspCI CAANNNNNGTGG 2 cut(s) 83, 118
CviAII CATG 2 cut(s) 39, 407
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 2 cut(s) 140, 546
DpnI GATC 4 cut(s) 66, 192, 270, 422
DpnII GATC 4 cut(s) 64, 190, 268, 420
DraI TTTAAA 1 cut(s) 202
DrdI GACNNNNNNGTC 1 cut(s) 86
DseDI GACNNNNNNGTC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 490
Eco91I GGTNACC 1 cut(s) 236
EcoO65I GGTNACC 1 cut(s) 236
Esp3I CGTCTC 1 cut(s) 461
FaeI CATG 2 cut(s) 42, 410
FaiI YATR 8 cut(s) 40, 172, 296, 379, 384, 408, 438, 477
FalI AAGNNNNNCTT 4 cut(s) 170, 202, 488, 520
FaqI GGGAC 1 cut(s) 31
FatI CATG 2 cut(s) 38, 406
FbaI TGATCA 4 cut(s) 64, 190, 268, 420
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 2 cut(s) 214, 412
Fsp4HI GCNGC 2 cut(s) 214, 412
GluI GCNGC 2 cut(s) 214, 412
Hin1II CATG 2 cut(s) 42, 410
HindIII AAGCTT 1 cut(s) 137
HphI GGTGA 2 cut(s) 464, 553
Hpy166II GTNNAC 4 cut(s) 55, 91, 343, 402
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 2 cut(s) 311, 446
Hpy8I GTNNAC 4 cut(s) 55, 91, 343, 402
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 2 cut(s) 227, 490
HpyCH4III ACNGT 5 cut(s) 52, 250, 340, 455, 556
HpyCH4V TGCA 6 cut(s) 14, 33, 123, 177, 330, 402
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 120, 408, 490
HpyF3I CTNAG 2 cut(s) 140, 546
Hsp92II CATG 2 cut(s) 42, 410
Ksp22I TGATCA 4 cut(s) 64, 190, 268, 420
Kzo9I GATC 4 cut(s) 64, 190, 268, 420
LpnPI CCDG 9 cut(s) 38, 195, 244, 257, 288, 304, 382, 494, 509
Lsp1109I GCAGC 1 cut(s) 225
LweI GCATC 2 cut(s) 101, 478
MaeIII GTNAC 2 cut(s) 236, 541
MalI GATC 4 cut(s) 66, 192, 270, 422
MboI GATC 4 cut(s) 64, 190, 268, 420
MboII GAAGA 2 cut(s) 58, 539
MhlI GDGCHC 2 cut(s) 404, 453
MluCI AATT 1 cut(s) 145
MnlI CCTC 4 cut(s) 143, 154, 192, 532
MseI TTAA 1 cut(s) 201
MwoI GCNNNNNNNGC 4 cut(s) 11, 120, 408, 490
NdeII GATC 4 cut(s) 64, 190, 268, 420
NlaIII CATG 2 cut(s) 42, 410
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 541
NspI RCATGY 1 cut(s) 42
PkrI GCNGC 2 cut(s) 215, 413
PspEI GGTNACC 1 cut(s) 236
PspN4I GGNNCC 1 cut(s) 130
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 2 cut(s) 214, 412
Sau3AI GATC 4 cut(s) 64, 190, 268, 420
SduI GDGCHC 2 cut(s) 404, 453
SfaNI GCATC 2 cut(s) 101, 478
SmlI CTYRAG 2 cut(s) 134, 309
SmoI CTYRAG 2 cut(s) 134, 309
Sse9I AATT 1 cut(s) 145
SsiI CCGC 2 cut(s) 411, 560
TaaI ACNGT 5 cut(s) 52, 250, 340, 455, 556
TaqI TCGA 1 cut(s) 159
TasI AATT 1 cut(s) 145
TatI WGTACW 1 cut(s) 54
TauI GCSGC 1 cut(s) 414
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 3 cut(s) 79, 125, 561
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 213
Tsp45I GTSAC 1 cut(s) 541
TspRI CASTG 3 cut(s) 79, 125, 561
VneI GTGCAC 1 cut(s) 400
XceI RCATGY 1 cut(s) 42
XmiI GTMKAC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.