Rorug05G0538600

Zinc-binding domain present in Lin-11, Isl-1, Mec-3.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000896
Physical Location & Seq
Forward (+)
72731052 .. 72734660
3609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0538600.1

Sequence Viewer

Length: 1125 bp
ATGGAACACTGTTACGAGCAAAAAAAAGAAGCAGAGAGCTTCGAAAACCAGAAAGGCGAAAAAGCGAGAGAGAGAGAGAGAGAGAGGCTGAAACCCCCAAATCGGAGAAGAATGGCGGAGTCGTCGAGCAGTGGTCTCACGTTCAAGCTTCACCCTCTGGTCATGGTCAACATCTCCGACCACTACTCCCGAGTCAAGTCTCAGATGAACCCTCCCATCGCCGCCCACAACGCCACCGCAACAACCACCGCCACCAACAACAACAACAACAACGGCGCCGAAGCAGTCGAAACGGCGTCGTCTTCCTCCTCCTCCTCCTCCGCTTCGTCTCCGAGAGTCTTCGGCTGCGTCATCGGCGTCCAGCGTGGTCGCACCGTCGAGATCTTCAACAGCTTCGAGCTCCTCTACGATCCGGAGACTCACTCGCTCGAGCGTGGCTTCCTCGAGAAGAAACAAGAGCTCTATAAAAAGGTTTTCCCCCATTTTTACATACTGGGATGGTACTCTACCGGCTCTGATGCTCAGGAATCTGATATGACTATTCACAAAGCTTTGATGGATATCAATGAAAGCCCTGTTTATGTTCTCCTCAATCCTGTGATCAATCCTGCTCAGAAGGACCTGCCAATTACTATTTACGAAAGTGAGCTGCATGTCATTGATGGGGTTCCGCAGCTAATTTTTGTCAGTTCAAGCTACACAATTGAGACGGTAGAAGCTGAACGTATCTCAGTTGATCATGTTGCTCATCTTAAACCATCTGATGGAGGTTCAGCAGCAACTCAATTGGCTGCTCATCTTACAGGAATCCACAGTGCCATCAAGATGCTCAATAGCAGAATTAGAGTGCTTCATCACTATCTTCTAGCTATGCAGAAAGGAGAAATACCTTGTGAAAATTCGCTGCTAAGACAAGTATCAAGTCTTCTCAGAAGGTTGCCTGCTGTTGAATCGGGGAAATTTCAAGATGACTTTTTGATGGAATACAATGACACATTGCTGATTACATATCTAGCTGTGCTTACCAACTGCTCAAGTACAATGAATGAGCTGGTCGACAAGTTCAACACCGCTTATGACAGGCACAGCCGAAGAGGTGGAGGAGGACGTACTGCTTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIM

Protein Analysis

374

Amino Acids

41.87

Weight (kDa)

6.44

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
JAB PF01398 100 - 190 2.9e-15 JAB1/Mov34/MPN/PAD-1 ubiquitin protease
MitMem_reg PF13012 239 - 353 7.4e-32 Maintenance of mitochondrial structure and function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 630
AccB1I GGYRCC 1 cut(s) 275
AccB7I CCANNNNNTGG 1 cut(s) 764
AccI GTMKAC 1 cut(s) 1056
AccIII TCCGGA 1 cut(s) 412
AciI CCGC 7 cut(s) 116, 222, 237, 249, 321, 671, 1071
AclWI GGATC 1 cut(s) 404
AcsI RAATTY 2 cut(s) 898, 959
AcyI GRCGYC 3 cut(s) 276, 296, 357
AfaI GTAC 3 cut(s) 503, 1039, 1111
AfiI CCNNNNNNNGG 2 cut(s) 102, 764
AgsI TTSAA 6 cut(s) 145, 388, 693, 950, 965, 1066
Alw21I GWGCWC 2 cut(s) 402, 462
Alw26I GTCTC 5 cut(s) 140, 204, 333, 410, 701
AlwI GGATC 1 cut(s) 404
Ama87I CYCGRG 3 cut(s) 189, 428, 443
Aor13HI TCCGGA 1 cut(s) 412
ApeKI GCWGC 6 cut(s) 345, 649, 673, 776, 791, 904
ApoI RAATTY 2 cut(s) 898, 959
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 619
AsuHPI GGTGA 1 cut(s) 143
AsuII TTCGAA 1 cut(s) 42
AvaI CYCGRG 3 cut(s) 189, 428, 443
AvaII GGWCC 1 cut(s) 619
BanI GGYRCC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 402, 462
BarI GAAGNNNNNNTAC 2 cut(s) 909, 941
BbsI GAAGAC 3 cut(s) 294, 331, 917
Bbv12I GWGCWC 2 cut(s) 402, 462
BbvI GCAGC 6 cut(s) 332, 636, 685, 778, 788, 891
BccI CCATC 8 cut(s) 224, 492, 550, 656, 758, 766, 827, 973
BceAI ACGGC 2 cut(s) 289, 309
BclI TGATCA 2 cut(s) 600, 736
BcoDI GTCTC 5 cut(s) 140, 204, 333, 410, 701
BfaI CTAG 2 cut(s) 866, 1013
BfoI RGCGCY 1 cut(s) 279
BfuAI ACCTGC 1 cut(s) 630
BglII AGATCT 1 cut(s) 381
BisI GCNGC 7 cut(s) 222, 346, 650, 674, 777, 792, 905
BlsI GCNGC 7 cut(s) 223, 347, 651, 675, 778, 793, 906
Bme18I GGWCC 1 cut(s) 619
BmeT110I CYCGRG 3 cut(s) 189, 428, 443
BmgT120I GGNCC 1 cut(s) 619
BmiI GGNNCC 2 cut(s) 277, 669
BmrI ACTGGG 1 cut(s) 503
BmsI GCATC 2 cut(s) 508, 816
BmuI ACTGGG 1 cut(s) 503
BpiI GAAGAC 3 cut(s) 294, 331, 917
BplI GAGNNNNNCTC 2 cut(s) 407, 439
Bpu10I CCTNAGC 1 cut(s) 522
Bpu14I TTCGAA 1 cut(s) 42
BpuEI CTTGAG 1 cut(s) 1018
BsaBI GATNNNNATC 1 cut(s) 560
BsaHI GRCGYC 3 cut(s) 276, 296, 357
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 764
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 1 cut(s) 498
Bse3DI GCAATG 1 cut(s) 995
Bse8I GATNNNNATC 1 cut(s) 560
BseAI TCCGGA 1 cut(s) 412
BseGI GGATG 1 cut(s) 503
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 2 cut(s) 102, 764
BseMI GCAATG 1 cut(s) 995
BseMII CTCAG 5 cut(s) 215, 536, 626, 744, 943
BseNI ACTGG 1 cut(s) 498
BseRI GAGGAG 7 cut(s) 298, 301, 304, 307, 392, 578, 1116
BseXI GCAGC 6 cut(s) 332, 636, 685, 778, 788, 891
BshNI GGYRCC 1 cut(s) 275
BsiHKAI GWGCWC 2 cut(s) 402, 462
BsiHKCI CYCGRG 3 cut(s) 189, 428, 443
BsiSI CCGG 2 cut(s) 413, 510
BslI CCNNNNNNNGG 2 cut(s) 102, 764
BsmAI GTCTC 5 cut(s) 140, 204, 333, 410, 701
BsmBI CGTCTC 2 cut(s) 333, 701
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 3 cut(s) 189, 428, 443
Bsp119I TTCGAA 1 cut(s) 42
Bsp1286I GDGCHC 2 cut(s) 402, 462
Bsp13I TCCGGA 1 cut(s) 412
Bsp143I GATC 4 cut(s) 381, 409, 600, 736
BspACI CCGC 7 cut(s) 116, 222, 237, 249, 321, 671, 1071
BspCNI CTCAG 5 cut(s) 214, 535, 625, 743, 942
BspEI TCCGGA 1 cut(s) 412
BspLI GGNNCC 2 cut(s) 277, 669
BspMI ACCTGC 1 cut(s) 630
BspPI GGATC 1 cut(s) 404
BspT104I TTCGAA 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 995
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 1 cut(s) 498
BssAI RCCGGY 1 cut(s) 509
BssMI GATC 4 cut(s) 381, 409, 600, 736
BssNI GRCGYC 3 cut(s) 276, 296, 357
Bst4CI ACNGT 4 cut(s) 11, 376, 712, 815
Bst6I CTCTTC 1 cut(s) 1087
BstACI GRCGYC 3 cut(s) 276, 296, 357
BstBI TTCGAA 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 942
BstDEI CTNAG 6 cut(s) 201, 522, 612, 730, 908, 929
BstF5I GGATG 1 cut(s) 503
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 4 cut(s) 384, 412, 603, 739
BstMAI GTCTC 5 cut(s) 140, 204, 333, 410, 701
BstMBI GATC 4 cut(s) 381, 409, 600, 736
BstMWI GCNNNNNNNGC 2 cut(s) 230, 354
BstNSI RCATGY 1 cut(s) 656
BstV1I GCAGC 6 cut(s) 332, 636, 685, 778, 788, 891
BstV2I GAAGAC 3 cut(s) 294, 331, 917
BstX2I RGATCY 1 cut(s) 381
BstYI RGATCY 1 cut(s) 381
BtgZI GCGATG 1 cut(s) 202
BtsCI GGATG 1 cut(s) 503
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 3 cut(s) 7, 136, 820
BveI ACCTGC 1 cut(s) 630
Cac8I GCNNGC 1 cut(s) 942
CfoI GCGC 1 cut(s) 278
Cfr10I RCCGGY 1 cut(s) 509
Cfr13I GGNCC 1 cut(s) 619
CseI GACGC 3 cut(s) 285, 337, 346
Csp6I GTAC 3 cut(s) 502, 1038, 1110
CviAII CATG 3 cut(s) 163, 653, 740
CviQI GTAC 3 cut(s) 502, 1038, 1110
DdeI CTNAG 6 cut(s) 201, 522, 612, 730, 908, 929
DinI GGCGCC 1 cut(s) 277
DpnI GATC 4 cut(s) 383, 411, 602, 738
DpnII GATC 4 cut(s) 381, 409, 600, 736
Eam1104I CTCTTC 1 cut(s) 1087
EarI CTCTTC 1 cut(s) 1087
EciI GGCGGA 1 cut(s) 131
Ecl136II GAGCTC 2 cut(s) 400, 460
Eco24I GRGCYC 2 cut(s) 402, 462
Eco31I GGTCTC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 619
Eco53kI GAGCTC 2 cut(s) 400, 460
Eco88I CYCGRG 3 cut(s) 189, 428, 443
EcoICRI GAGCTC 2 cut(s) 400, 460
EcoO109I RGGNCCY 1 cut(s) 619
EcoRV GATATC 1 cut(s) 562
EcoT38I GRGCYC 2 cut(s) 402, 462
EgeI GGCGCC 1 cut(s) 277
EheI GGCGCC 1 cut(s) 277
Esp3I CGTCTC 2 cut(s) 333, 701
FaeI CATG 3 cut(s) 166, 656, 743
FatI CATG 3 cut(s) 162, 652, 739
FbaI TGATCA 2 cut(s) 600, 736
FblI GTMKAC 1 cut(s) 1056
Fnu4HI GCNGC 7 cut(s) 222, 346, 650, 674, 777, 792, 905
FokI GGATG 1 cut(s) 510
FriOI GRGCYC 2 cut(s) 402, 462
Fsp4HI GCNGC 7 cut(s) 222, 346, 650, 674, 777, 792, 905
FspBI CTAG 2 cut(s) 866, 1013
GlaI GCGC 1 cut(s) 277
GluI GCNGC 7 cut(s) 222, 346, 650, 674, 777, 792, 905
HaeII RGCGCY 1 cut(s) 279
HapII CCGG 2 cut(s) 413, 510
HgaI GACGC 3 cut(s) 285, 337, 346
HhaI GCGC 1 cut(s) 278
Hin1I GRCGYC 3 cut(s) 276, 296, 357
Hin1II CATG 3 cut(s) 166, 656, 743
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HincII GTYRAC 2 cut(s) 169, 1057
HindII GTYRAC 2 cut(s) 169, 1057
HindIII AAGCTT 2 cut(s) 146, 549
HinfI GANTC 7 cut(s) 119, 192, 336, 418, 527, 807, 950
HpaII CCGG 2 cut(s) 413, 510
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 2 cut(s) 169, 1057
Hpy188III TCNNGA 7 cut(s) 189, 379, 413, 445, 524, 823, 965
Hpy8I GTNNAC 2 cut(s) 169, 1057
Hpy99I CGWCG 3 cut(s) 127, 301, 380
HpyAV CCTTC 2 cut(s) 610, 927
HpyCH4III ACNGT 4 cut(s) 11, 376, 712, 815
HpyCH4IV ACGT 3 cut(s) 140, 724, 1108
HpyCH4V TGCA 2 cut(s) 652, 874
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 354
HpyF3I CTNAG 6 cut(s) 201, 522, 612, 730, 908, 929
HpySE526I ACGT 3 cut(s) 140, 724, 1108
Hsp92I GRCGYC 3 cut(s) 276, 296, 357
Hsp92II CATG 3 cut(s) 166, 656, 743
HspAI GCGC 1 cut(s) 276
KasI GGCGCC 1 cut(s) 275
Kpn2I TCCGGA 1 cut(s) 412
Ksp22I TGATCA 2 cut(s) 600, 736
Kzo9I GATC 4 cut(s) 381, 409, 600, 736
LmnI GCTCC 1 cut(s) 405
Lsp1109I GCAGC 6 cut(s) 332, 636, 685, 778, 788, 891
LweI GCATC 2 cut(s) 508, 816
MaeI CTAG 2 cut(s) 866, 1013
MaeII ACGT 3 cut(s) 140, 724, 1108
MaeIII GTNAC 1 cut(s) 11
MalI GATC 4 cut(s) 383, 411, 602, 738
MboI GATC 4 cut(s) 381, 409, 600, 736
MboII GAAGA 9 cut(s) 120, 294, 331, 376, 460, 854, 917, 1104, 1111
MfeI CAATTG 2 cut(s) 702, 785
MflI RGATCY 1 cut(s) 381
MhlI GDGCHC 2 cut(s) 402, 462
MluCI AATT 7 cut(s) 627, 678, 702, 785, 840, 898, 959
Mly113I GGCGCC 1 cut(s) 276
MlyI GAGTC 4 cut(s) 128, 201, 345, 412
MmeI TCCRAC 1 cut(s) 201
MroI TCCGGA 1 cut(s) 412
MseI TTAA 1 cut(s) 753
MslI CAYNNNNRTG 1 cut(s) 824
MspI CCGG 2 cut(s) 413, 510
MunI CAATTG 2 cut(s) 702, 785
MwoI GCNNNNNNNGC 2 cut(s) 230, 354
NarI GGCGCC 1 cut(s) 276
NdeII GATC 4 cut(s) 381, 409, 600, 736
NlaIII CATG 3 cut(s) 166, 656, 743
NlaIV GGNNCC 2 cut(s) 277, 669
NspI RCATGY 1 cut(s) 656
NspV TTCGAA 1 cut(s) 42
PaeR7I CTCGAG 2 cut(s) 428, 443
PfeI GAWTC 3 cut(s) 527, 807, 950
PflMI CCANNNNNTGG 1 cut(s) 764
PkrI GCNGC 7 cut(s) 223, 347, 651, 675, 778, 793, 906
PleI GAGTC 4 cut(s) 127, 200, 344, 412
PluTI GGCGCC 1 cut(s) 279
PpsI GAGTC 4 cut(s) 127, 200, 344, 412
PpuMI RGGWCCY 1 cut(s) 619
Psp124BI GAGCTC 2 cut(s) 402, 462
Psp5II RGGWCCY 1 cut(s) 619
PspN4I GGNNCC 2 cut(s) 277, 669
PspPI GGNCC 1 cut(s) 619
PspPPI RGGWCCY 1 cut(s) 619
PspXI VCTCGAGB 1 cut(s) 428
PsuI RGATCY 1 cut(s) 381
RsaI GTAC 3 cut(s) 503, 1039, 1111
RsaNI GTAC 3 cut(s) 502, 1038, 1110
RseI CAYNNNNRTG 1 cut(s) 824
SacI GAGCTC 2 cut(s) 402, 462
SalI GTCGAC 1 cut(s) 1055
SaqAI TTAA 1 cut(s) 753
SatI GCNGC 7 cut(s) 222, 346, 650, 674, 777, 792, 905
Sau3AI GATC 4 cut(s) 381, 409, 600, 736
Sau96I GGNCC 1 cut(s) 619
SchI GAGTC 4 cut(s) 128, 201, 345, 412
SduI GDGCHC 2 cut(s) 402, 462
SfaNI GCATC 2 cut(s) 508, 816
SfoI GGCGCC 1 cut(s) 277
Sfr274I CTCGAG 2 cut(s) 428, 443
SfuI TTCGAA 1 cut(s) 42
SinI GGWCC 1 cut(s) 619
SlaI CTCGAG 2 cut(s) 428, 443
SmiMI CAYNNNNRTG 1 cut(s) 824
SmlI CTYRAG 3 cut(s) 428, 443, 1033
SmoI CTYRAG 3 cut(s) 428, 443, 1033
Sse9I AATT 7 cut(s) 627, 678, 702, 785, 840, 898, 959
SsiI CCGC 7 cut(s) 116, 222, 237, 249, 321, 671, 1071
SspDI GGCGCC 1 cut(s) 275
SspMI CTAG 2 cut(s) 866, 1013
SstI GAGCTC 2 cut(s) 402, 462
TaaI ACNGT 4 cut(s) 11, 376, 712, 815
TaiI ACGT 3 cut(s) 143, 727, 1111
TaqI TCGA 8 cut(s) 42, 125, 288, 378, 396, 429, 444, 1056
TasI AATT 7 cut(s) 627, 678, 702, 785, 840, 898, 959
TatI WGTACW 1 cut(s) 1037
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 3 cut(s) 527, 807, 950
Tru1I TTAA 1 cut(s) 753
Tru9I TTAA 1 cut(s) 753
TscAI CASTG 3 cut(s) 14, 136, 820
TseI GCWGC 6 cut(s) 345, 649, 673, 776, 791, 904
TspDTI ATGAA 4 cut(s) 221, 582, 842, 1058
TspRI CASTG 3 cut(s) 14, 136, 820
Van91I CCANNNNNTGG 1 cut(s) 764
VpaK11BI GGWCC 1 cut(s) 619
XapI RAATTY 2 cut(s) 898, 959
XceI RCATGY 1 cut(s) 656
XhoI CTCGAG 2 cut(s) 428, 443
XmiI GTMKAC 1 cut(s) 1056
XspI CTAG 2 cut(s) 866, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.