Rorug05G0538700

Zinc-binding domain present in Lin-11, Isl-1, Mec-3.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000896
Physical Location & Seq
Reverse (-)
72735494 .. 72739013
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0538700.1

Sequence Viewer

Length: 2508 bp
ATGAGAGCTTTAGCAAAGCACACCATTCTTCTTCTCCTGACTTTACTCTCTTTCTTCTCCATTGCCAAATCCAAAGACACCTTATCTGCTACTGAATCGCTAGGAACAGGCCTCGTCTCTGCCGGCGAGGTTTTCGAGTTGGGTTTTTTCAACCCTGGCAGACACTCAAGATGGTACTTGGGCATATGGTACATGAAAATCAAAGAAAGAACAGTTGTTTGGGTGGCCAACAGAGACAATCCGCTCCCAAACAACTCAGCCAGTCTCAAAATCGGATACGACGGAATACTTTCTCTTGTGGATGAATCCCGAAAAGTTTCTTGGATGTCAAGCAATCAATCACAATTTGTTAAAAATCCAATCCTTCAGCTTTTGGATTCCGGCAACTTGGTCCTCAAAGAAGCAAATGAAACCAACCCTAACGAGTTCCTCTGGCAGAGCTTCGACTACCCTACACACACTTTGTTGCCGGAGATGAAGCTCGGAAGGAACTCGAATACCGGATTGGATAGATACATAACCTCGTGGAAAACTCCACAAGACCCTTCTACGGGTGATTACTCCTTCAAGCTCGACTACCGCGGTTTCCCGGAGGCTTTCCTCCGGCAGAGACAGTCGATAAAGTACCGGAGCGGCCCCTGGAACGGGTTGAGATTTAGCGGGGCTCCGGAGATGAACACCGCTAGCTCTAATGGTATTGGTTTCAACTTCGTTGTAAACAATGAGGAGATGTACTACTCTTTTTCGGAAACTAATCCAGCTGTGTATTCACGGCTGATTGCATCTCCCTCCGGCAACATCCAACGGCTGACGTGGATCGAGAGCCGCAAGACCTGGAGCCAGTGCTGGTACCTTCCCAGGGACCCATGCGACACGTACAGCGAGTGCGGGCCCTATGGTGTCTGCGACGCCAACGATGCTTTGCCGGTGTGTAAGTGCATGAGGGGCTTCCGACCTAAGAAGCCTTCTGCATGGAGCTTGAGAGATGGCACCGAAGGATGTGAACGTCAGACGGAGCTGGAGTGTGGGAGTAAGGACAAGTTTATGAAGATGGAGAATTTGAAGCTGCCGGAGAGCGGCGGAGCGGTGGTGGATATGGAGATGAGTTTGGAGGAGTGTGAGGTGAAGTGTTTGGGGAATTGCTCGTGTAGTGGGTTTTCGAGTGCGAGGATTGAGAAAGGAGGGAGTGGATGTGTGATGTGGTTTGGGAAGTTGATGGATATGAGGAGTTATGCCGGCGGCAGCGGCCAAGAGTTCTATCTCCGATTAGCAGCTTCTGAACTAGATGGTGATGGGAAAACAAAACGCATGGTTATGAGCGTAGGCATTGTAGTTGGTATTGCCGCTCTGCTATCAGCTGGTCTTATTATCTGGTTCATGCGGAGGAGGAACTCGGGTAGCACCCTGAGGAGAGAATCAAAAGGTCCTCTTGAAAGAATTCGAAGCCAGGATTTTCTTCTAAACAGTGTGTTAGTCTCAAGTAAGAAGGAGCACTACTCTGGTGACAGAAGCAATGATGACCTAGAGTTGCCATTGTTTGATTTTACCTCCATAGCAGCGGCTACGGAGAACTTTTCTGATGAAAATAAACTGGGACAAGGTGGTTTTGGTTGTGTTTACAAGGGGTTTGTCGAAGGTCAAGAGATAGCTGTGAAGAGACTTTCAAAGAACTCTGGACAAGGAACCAAAGAATTCAAAAACGAAGTGAAGTTAATTGCAAGGCTTCAACACAGAAATCTTGTTCGACTGCTTGGTTGCTGTGTTGATGCGGATGAAAAGATGCTGATTTACGAATACATGGAAAATAAAAGCCTCGATTCCTTTTTATTCGATAAAGCAAAAAGGTATTTGCTGGATTGGCAAAAGCGTTTCAACATTATCTGTGGGATTGCTCGAGGGCTTCTTTATCTTCACCAGGATTCGAGATTCAGAATTATCCATAGGGACCTCAAGGCAAGCAACATTCTACTAGATGGAGAATTGGACCCCAAGATATCGGACTTTGGAATGGCAAGACTATTCGGTCAGGATCAGACAGAAGCCAATACAAAAAAAGTAGTGGGAACATATGGCTATATGTCTCCTGAATACGCAATGGATGGTCTCTTCTCAATCAAGTCGGATGTATTTAGTTTTGGTGTTCTAGTATTGGAGATTGTTAGTGGTCAAAAGAACAGAGGATTCTATTATTCAAACAATGAGCTAAACCTCCTAGGACATTTTTGGGAGCTATGGAATGGAGGTCGTGCATTGGATATAATAGATTCATCAGTTGGTGATTCATTTTCGGAATCTGAGGTGTTGAGAAGCATGCAGGTAGGGCTTTTATGCGTCCAAGAGCGTGCAGAAGATAGGCCTACAATGTCCTCTGTGGTTCTAATGTTATGTAGTGAAACTGCATCATTACCGCAGCCTAAAAAACCCAGTTTTTGCCTAGCAAGGAAACCCGAAACAGACGAGTCAAGCACTGTAAACCAAGTCACTATCACAATATTAGATCCTAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LIM

Protein Analysis

835

Amino Acids

93.86

Weight (kDa)

7.39

Isoelectric Point (pI)

45.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 177 3.7e-37 D-mannose binding lectin
S_locus_glycop PF00954 209 - 320 5.2e-33 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 9.3e-20 PAN-like domain
DUF3660 PF12398 479 - 515 4.2e-08 Receptor serine/threonine kinase
Pkinase PF00069 526 - 724 7.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 528 - 792 2.7e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 797 - 835 1.5e-12 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2022
Acc36I ACCTGC 1 cut(s) 2305
Acc65I GGTACC 1 cut(s) 849
AccB1I GGYRCC 2 cut(s) 849, 989
AccBSI CCGCTC 5 cut(s) 244, 633, 1077, 1085, 1346
AccII CGCG 1 cut(s) 582
AccIII TCCGGA 1 cut(s) 667
AclWI GGATC 3 cut(s) 824, 2037, 2492
AcoI YGGCCR 2 cut(s) 225, 1246
AcsI RAATTY 3 cut(s) 1057, 1437, 1691
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 1 cut(s) 909
AfaI GTAC 6 cut(s) 176, 191, 626, 734, 851, 878
AfiI CCNNNNNNNGG 6 cut(s) 550, 551, 644, 645, 859, 1076
AflIII ACRYGT 1 cut(s) 873
AjiI CACGTC 1 cut(s) 813
AjnI CCWGG 6 cut(s) 154, 638, 833, 857, 1446, 1914
AjuI GAANNNNNNNTTGG 6 cut(s) 202, 234, 304, 336, 488, 520
Alw21I GWGCWC 1 cut(s) 1494
Alw26I GTCTC 8 cut(s) 121, 228, 269, 604, 1480, 1651, 2085, 2108
AlwI GGATC 3 cut(s) 824, 2037, 2492
AlwNI CAGNNNCTG 1 cut(s) 1277
Ama87I CYCGRG 2 cut(s) 1393, 1893
Aor13HI TCCGGA 1 cut(s) 667
AoxI GGCC 6 cut(s) 109, 225, 634, 890, 1246, 2354
ApaI GGGCCC 1 cut(s) 894
ApeKI GCWGC 5 cut(s) 1066, 1242, 1271, 1556, 2410
ApoI RAATTY 3 cut(s) 1057, 1437, 1691
Asp700I GAANNNNTTC 3 cut(s) 289, 316, 1437
Asp718I GGTACC 1 cut(s) 849
AspA2I CCTAGG 2 cut(s) 2212, 2501
AspS9I GGNCC 8 cut(s) 391, 635, 862, 890, 891, 1424, 1945, 1984
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 6 cut(s) 566, 1135, 1301, 1514, 1904, 2288
AsuII TTCGAA 1 cut(s) 1441
AsuNHI GCTAGC 1 cut(s) 683
AvaI CYCGRG 2 cut(s) 1393, 1893
AvaII GGWCC 5 cut(s) 391, 862, 1424, 1945, 1984
AvrII CCTAGG 2 cut(s) 2212, 2501
AxyI CCTNAGG 1 cut(s) 1406
BaeGI GKGCMC 1 cut(s) 894
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 2 cut(s) 849, 989
BanII GRGCYC 2 cut(s) 667, 894
BarI GAAGNNNNNNTAC 2 cut(s) 1478, 1510
BauI CACGAG 2 cut(s) 523, 1144
Bbv12I GWGCWC 1 cut(s) 1494
BbvI GCAGC 5 cut(s) 1053, 1254, 1283, 1568, 2422
BccI CCATC 8 cut(s) 165, 980, 1045, 1210, 1280, 1286, 1967, 2093
BceAI ACGGC 2 cut(s) 788, 821
BciT130I CCWGG 6 cut(s) 156, 640, 835, 859, 1448, 1916
BciVI GTATCC 1 cut(s) 269
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 8 cut(s) 121, 228, 269, 604, 1480, 1651, 2085, 2108
BfaI CTAG 9 cut(s) 101, 684, 1283, 1523, 1970, 2144, 2213, 2435, 2502
BfuAI ACCTGC 1 cut(s) 2305
BfuI GTATCC 1 cut(s) 269
BlnI CCTAGG 2 cut(s) 2212, 2501
Bme1390I CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1448, 1916
Bme18I GGWCC 5 cut(s) 391, 862, 1424, 1945, 1984
BmeT110I CYCGRG 2 cut(s) 1393, 1893
BmgBI CACGTC 1 cut(s) 813
BmgT120I GGNCC 8 cut(s) 391, 635, 862, 890, 891, 1424, 1945, 1984
BmrFI CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1448, 1916
BmrI ACTGGG 2 cut(s) 1601, 2418
BmsI GCATC 5 cut(s) 791, 907, 1756, 1770, 2408
BmtI GCTAGC 1 cut(s) 687
BmuI ACTGGG 2 cut(s) 1601, 2418
BplI GAGNNNNNCTC 2 cut(s) 1481, 1513
BpmI CTGGAG 2 cut(s) 856, 1040
Bpu14I TTCGAA 1 cut(s) 1441
BpuEI CTTGAG 4 cut(s) 151, 1000, 1462, 1934
BpuMI CCSGG 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 876
BsaHI GRCGYC 1 cut(s) 909
BsaI GGTCTC 1 cut(s) 2108
BsaJI CCNNGG 7 cut(s) 154, 580, 638, 857, 858, 2212, 2501
BsaWI WCCGGW 3 cut(s) 500, 627, 667
BsaXI ACNNNNNCTCC 6 cut(s) 1007, 1037, 1074, 1104, 1177, 1207
Bsc4I CCNNNNNNNGG 6 cut(s) 550, 551, 644, 645, 859, 1076
Bse118I RCCGGY 3 cut(s) 122, 925, 1235
Bse1I ACTGG 4 cut(s) 261, 841, 1596, 2424
Bse21I CCTNAGG 1 cut(s) 1406
Bse3DI GCAATG 3 cut(s) 60, 1519, 2100
BseAI TCCGGA 1 cut(s) 667
BseBI CCWGG 6 cut(s) 156, 640, 835, 859, 1448, 1916
BseDI CCNNGG 7 cut(s) 154, 580, 638, 857, 858, 2212, 2501
BseGI GGATG 8 cut(s) 307, 330, 798, 1004, 1196, 1777, 2104, 2128
BseLI CCNNNNNNNGG 6 cut(s) 550, 551, 644, 645, 859, 1076
BseMI GCAATG 3 cut(s) 60, 1519, 2100
BseMII CTCAG 3 cut(s) 270, 1397, 2286
BseNI ACTGG 4 cut(s) 261, 841, 1596, 2424
BseRI GAGGAG 5 cut(s) 740, 1127, 1240, 1399, 1423
BseSI GKGCMC 1 cut(s) 894
BseXI GCAGC 5 cut(s) 1053, 1254, 1283, 1568, 2422
BsgI GTGCAG 1 cut(s) 2364
Bsh1236I CGCG 1 cut(s) 582
BshFI GGCC 6 cut(s) 111, 227, 636, 892, 1248, 2356
BshNI GGYRCC 2 cut(s) 849, 989
BsiHKAI GWGCWC 1 cut(s) 1494
BsiHKCI CYCGRG 2 cut(s) 1393, 1893
BslFI GGGAC 3 cut(s) 875, 1608, 1958
BslI CCNNNNNNNGG 6 cut(s) 550, 551, 644, 645, 859, 1076
BsmAI GTCTC 8 cut(s) 121, 228, 269, 604, 1480, 1651, 2085, 2108
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 3 cut(s) 875, 1608, 1958
BsnI GGCC 6 cut(s) 111, 227, 636, 892, 1248, 2356
Bso31I GGTCTC 1 cut(s) 2108
BsoBI CYCGRG 2 cut(s) 1393, 1893
Bsp119I TTCGAA 1 cut(s) 1441
Bsp120I GGGCCC 1 cut(s) 890
Bsp1286I GDGCHC 3 cut(s) 667, 894, 1494
Bsp13I TCCGGA 1 cut(s) 667
Bsp143I GATC 3 cut(s) 816, 2029, 2497
BspANI GGCC 6 cut(s) 111, 227, 636, 892, 1248, 2356
BspCNI CTCAG 3 cut(s) 269, 1398, 2287
BspEI TCCGGA 1 cut(s) 667
BspFNI CGCG 1 cut(s) 582
BspMI ACCTGC 1 cut(s) 2305
BspOI GCTAGC 1 cut(s) 687
BspPI GGATC 3 cut(s) 824, 2037, 2492
BspT104I TTCGAA 1 cut(s) 1441
BspT107I GGYRCC 2 cut(s) 849, 989
BspTNI GGTCTC 1 cut(s) 2108
BsrBI CCGCTC 5 cut(s) 244, 633, 1077, 1085, 1346
BsrDI GCAATG 3 cut(s) 60, 1519, 2100
BsrFI RCCGGY 3 cut(s) 122, 925, 1235
BsrI ACTGG 4 cut(s) 261, 841, 1596, 2424
BssAI RCCGGY 3 cut(s) 122, 925, 1235
BssECI CCNNGG 7 cut(s) 154, 580, 638, 857, 858, 2212, 2501
BssMI GATC 3 cut(s) 816, 2029, 2497
BssNI GRCGYC 1 cut(s) 909
BssSI CACGAG 2 cut(s) 523, 1144
BssT1I CCWWGG 2 cut(s) 2212, 2501
Bst2BI CACGAG 2 cut(s) 523, 1144
Bst2UI CCWGG 6 cut(s) 156, 640, 835, 859, 1448, 1916
Bst4CI ACNGT 4 cut(s) 214, 615, 1466, 2470
Bst6I CTCTTC 2 cut(s) 1649, 2111
BstACI GRCGYC 1 cut(s) 909
BstBAI YACGTR 1 cut(s) 876
BstBI TTCGAA 1 cut(s) 1441
BstC8I GCNNGC 7 cut(s) 124, 685, 890, 1237, 1957, 2312, 2343
BstDEI CTNAG 4 cut(s) 256, 957, 1406, 2295
BstDSI CCRYGG 1 cut(s) 580
BstF5I GGATG 8 cut(s) 307, 330, 798, 1004, 1196, 1777, 2104, 2128
BstFNI CGCG 1 cut(s) 582
BstKTI GATC 3 cut(s) 819, 2032, 2500
BstMAI GTCTC 8 cut(s) 121, 228, 269, 604, 1480, 1651, 2085, 2108
BstMBI GATC 3 cut(s) 816, 2029, 2497
BstMWI GCNNNNNNNGC 5 cut(s) 917, 945, 1245, 1858, 2320
BstNI CCWGG 6 cut(s) 156, 640, 835, 859, 1448, 1916
BstNSI RCATGY 1 cut(s) 2314
BstSCI CCNGG 7 cut(s) 154, 588, 638, 833, 857, 1446, 1914
BstSLI GKGCMC 1 cut(s) 894
BstUI CGCG 1 cut(s) 582
BstV1I GCAGC 5 cut(s) 1053, 1254, 1283, 1568, 2422
BstX2I RGATCY 1 cut(s) 2497
BstYI RGATCY 1 cut(s) 2497
Bsu36I CCTNAGG 1 cut(s) 1406
BsuI GTATCC 1 cut(s) 269
BsuRI GGCC 6 cut(s) 111, 227, 636, 892, 1248, 2356
BtgI CCRYGG 1 cut(s) 580
BtrI CACGTC 1 cut(s) 813
BtsCI GGATG 8 cut(s) 307, 330, 798, 1004, 1196, 1777, 2104, 2128
BtsIMutI CAGTG 3 cut(s) 848, 1471, 2466
BveI ACCTGC 1 cut(s) 2305
Cac8I GCNNGC 7 cut(s) 124, 685, 890, 1237, 1957, 2312, 2343
CaiI CAGNNNCTG 1 cut(s) 1277
Cfr10I RCCGGY 3 cut(s) 122, 925, 1235
Cfr13I GGNCC 8 cut(s) 391, 635, 862, 890, 891, 1424, 1945, 1984
Cfr42I CCGCGG 1 cut(s) 583
CseI GACGC 2 cut(s) 917, 2320
Csp6I GTAC 6 cut(s) 175, 190, 625, 733, 850, 877
CviAII CATG 8 cut(s) 193, 867, 940, 972, 1309, 1378, 1798, 2311
CviQI GTAC 6 cut(s) 175, 190, 625, 733, 850, 877
DdeI CTNAG 4 cut(s) 256, 957, 1406, 2295
DpnI GATC 3 cut(s) 818, 2031, 2499
DpnII GATC 3 cut(s) 816, 2029, 2497
DrdI GACNNNNNNGTC 1 cut(s) 2022
DseDI GACNNNNNNGTC 1 cut(s) 2022
EaeI YGGCCR 2 cut(s) 225, 1246
Eam1104I CTCTTC 2 cut(s) 1649, 2111
EarI CTCTTC 2 cut(s) 1649, 2111
EciI GGCGGA 1 cut(s) 1095
Eco130I CCWWGG 2 cut(s) 2212, 2501
Eco147I AGGCCT 2 cut(s) 111, 2356
Eco24I GRGCYC 2 cut(s) 667, 894
Eco31I GGTCTC 1 cut(s) 2108
Eco32I GATATC 1 cut(s) 1995
Eco47I GGWCC 5 cut(s) 391, 862, 1424, 1945, 1984
Eco57I CTGAAG 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 1406
Eco88I CYCGRG 2 cut(s) 1393, 1893
EcoO109I RGGNCCY 4 cut(s) 862, 891, 1424, 1945
EcoRI GAATTC 2 cut(s) 1437, 1691
EcoRII CCWGG 6 cut(s) 154, 638, 833, 857, 1446, 1914
EcoRV GATATC 1 cut(s) 1995
EcoT14I CCWWGG 2 cut(s) 2212, 2501
EcoT38I GRGCYC 2 cut(s) 667, 894
ErhI CCWWGG 2 cut(s) 2212, 2501
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 8 cut(s) 196, 870, 943, 975, 1312, 1381, 1801, 2314
FalI AAGNNNNNCTT 2 cut(s) 1413, 1445
FaqI GGGAC 3 cut(s) 875, 1608, 1958
FatI CATG 8 cut(s) 192, 866, 939, 971, 1308, 1377, 1797, 2310
FauI CCCGC 2 cut(s) 653, 881
FauNDI CATATG 2 cut(s) 185, 2068
FokI GGATG 8 cut(s) 314, 337, 785, 1011, 1203, 1784, 2111, 2135
FriOI GRGCYC 2 cut(s) 667, 894
FspBI CTAG 9 cut(s) 101, 684, 1283, 1523, 1970, 2144, 2213, 2435, 2502
GsuI CTGGAG 2 cut(s) 856, 1040
HaeIII GGCC 6 cut(s) 111, 227, 636, 892, 1248, 2356
HgaI GACGC 2 cut(s) 917, 2320
Hin1I GRCGYC 1 cut(s) 909
Hin1II CATG 8 cut(s) 196, 870, 943, 975, 1312, 1381, 1801, 2314
HphI GGTGA 6 cut(s) 566, 1135, 1301, 1514, 1904, 2288
Hpy166II GTNNAC 4 cut(s) 718, 1004, 1618, 2473
Hpy8I GTNNAC 4 cut(s) 718, 1004, 1618, 2473
Hpy99I CGWCG 2 cut(s) 284, 911
HpyAV CCTTC 9 cut(s) 374, 480, 555, 574, 863, 975, 989, 1480, 1628
HpyCH4III ACNGT 4 cut(s) 214, 615, 1466, 2470
HpyCH4IV ACGT 3 cut(s) 812, 875, 1006
HpyCH4V TGCA 8 cut(s) 782, 939, 971, 1718, 2249, 2314, 2345, 2399
HpyF10VI GCNNNNNNNGC 5 cut(s) 917, 945, 1245, 1858, 2320
HpyF3I CTNAG 4 cut(s) 256, 957, 1406, 2295
HpySE526I ACGT 3 cut(s) 812, 875, 1006
Hsp92I GRCGYC 1 cut(s) 909
Hsp92II CATG 8 cut(s) 196, 870, 943, 975, 1312, 1381, 1801, 2314
KflI GGGWCCC 1 cut(s) 862
Kpn2I TCCGGA 1 cut(s) 667
KpnI GGTACC 1 cut(s) 853
KroI GCCGGC 2 cut(s) 122, 1235
KroNI GCCGGC 2 cut(s) 124, 1237
KspI CCGCGG 1 cut(s) 583
Kzo9I GATC 3 cut(s) 816, 2029, 2497
LmnI GCTCC 9 cut(s) 249, 630, 670, 837, 975, 1015, 1082, 1489, 2227
Lsp1109I GCAGC 5 cut(s) 1053, 1254, 1283, 1568, 2422
LweI GCATC 5 cut(s) 791, 907, 1756, 1770, 2408
MaeI CTAG 9 cut(s) 101, 684, 1283, 1523, 1970, 2144, 2213, 2435, 2502
MaeII ACGT 3 cut(s) 812, 875, 1006
MaeIII GTNAC 2 cut(s) 1502, 2479
MalI GATC 3 cut(s) 818, 2031, 2499
MbiI CCGCTC 5 cut(s) 244, 633, 1077, 1085, 1346
MboI GATC 3 cut(s) 816, 2029, 2497
MboII GAAGA 9 cut(s) 20, 23, 46, 1060, 1448, 1666, 1901, 2098, 2360
MflI RGATCY 1 cut(s) 2497
MhlI GDGCHC 3 cut(s) 667, 894, 1494
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 8 cut(s) 344, 1057, 1138, 1437, 1691, 1713, 1932, 1979
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 1 cut(s) 2468
MmeI TCCRAC 3 cut(s) 826, 976, 2100
Mox20I TGGCCA 1 cut(s) 227
MroI TCCGGA 1 cut(s) 667
MroNI GCCGGC 2 cut(s) 122, 1235
MroXI GAANNNNTTC 3 cut(s) 289, 316, 1437
MscI TGGCCA 1 cut(s) 227
MseI TTAA 2 cut(s) 351, 1712
MslI CAYNNNNRTG 1 cut(s) 1313
Msp20I TGGCCA 1 cut(s) 227
MspA1I CMGCKG 5 cut(s) 582, 761, 1245, 1358, 1559
MspR9I CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1448, 1916
MvaI CCWGG 6 cut(s) 156, 640, 835, 859, 1448, 1916
MvnI CGCG 1 cut(s) 582
MwoI GCNNNNNNNGC 5 cut(s) 917, 945, 1245, 1858, 2320
NaeI GCCGGC 2 cut(s) 124, 1237
NciI CCSGG 1 cut(s) 590
NdeI CATATG 2 cut(s) 185, 2068
NdeII GATC 3 cut(s) 816, 2029, 2497
NgoMIV GCCGGC 2 cut(s) 122, 1235
NheI GCTAGC 1 cut(s) 683
NlaIII CATG 8 cut(s) 196, 870, 943, 975, 1312, 1381, 1801, 2314
NmuCI GTSAC 2 cut(s) 1502, 2479
NspI RCATGY 1 cut(s) 2314
NspV TTCGAA 1 cut(s) 1441
PaeI GCATGC 1 cut(s) 2314
PaeR7I CTCGAG 1 cut(s) 1893
PasI CCCWGGG 1 cut(s) 858
PceI AGGCCT 2 cut(s) 111, 2356
PdiI GCCGGC 2 cut(s) 124, 1237
PdmI GAANNNNTTC 3 cut(s) 289, 316, 1437
PfoI TCCNGGA 1 cut(s) 588
PleI GAGTC 1 cut(s) 2467
PpsI GAGTC 1 cut(s) 2467
Ppu21I YACGTR 1 cut(s) 876
PpuMI RGGWCCY 3 cut(s) 862, 1424, 1945
Psp5II RGGWCCY 3 cut(s) 862, 1424, 1945
Psp6I CCWGG 6 cut(s) 154, 638, 833, 857, 1446, 1914
PspGI CCWGG 6 cut(s) 154, 638, 833, 857, 1446, 1914
PspOMI GGGCCC 1 cut(s) 890
PspPI GGNCC 8 cut(s) 391, 635, 862, 890, 891, 1424, 1945, 1984
PspPPI RGGWCCY 3 cut(s) 862, 1424, 1945
PspXI VCTCGAGB 1 cut(s) 1893
PstNI CAGNNNCTG 1 cut(s) 1277
PsuI RGATCY 1 cut(s) 2497
PvuII CAGCTG 2 cut(s) 761, 1358
RsaI GTAC 6 cut(s) 176, 191, 626, 734, 851, 878
RsaNI GTAC 6 cut(s) 175, 190, 625, 733, 850, 877
RseI CAYNNNNRTG 1 cut(s) 1313
SacII CCGCGG 1 cut(s) 583
SaqAI TTAA 2 cut(s) 351, 1712
Sau3AI GATC 3 cut(s) 816, 2029, 2497
Sau96I GGNCC 8 cut(s) 391, 635, 862, 890, 891, 1424, 1945, 1984
SchI GAGTC 1 cut(s) 2468
ScrFI CCNGG 7 cut(s) 156, 590, 640, 835, 859, 1448, 1916
SduI GDGCHC 3 cut(s) 667, 894, 1494
SfaNI GCATC 5 cut(s) 791, 907, 1756, 1770, 2408
Sfr274I CTCGAG 1 cut(s) 1893
Sfr303I CCGCGG 1 cut(s) 583
SfuI TTCGAA 1 cut(s) 1441
SgrBI CCGCGG 1 cut(s) 583
SinI GGWCC 5 cut(s) 391, 862, 1424, 1945, 1984
SlaI CTCGAG 1 cut(s) 1893
SmiMI CAYNNNNRTG 1 cut(s) 1313
SmlI CTYRAG 5 cut(s) 166, 979, 1477, 1893, 1949
SmoI CTYRAG 5 cut(s) 166, 979, 1477, 1893, 1949
SphI GCATGC 1 cut(s) 2314
Sse9I AATT 8 cut(s) 344, 1057, 1138, 1437, 1691, 1713, 1932, 1979
SseBI AGGCCT 2 cut(s) 111, 2356
SspI AATATT 1 cut(s) 2493
SspMI CTAG 9 cut(s) 101, 684, 1283, 1523, 1970, 2144, 2213, 2435, 2502
StuI AGGCCT 2 cut(s) 111, 2356
StyD4I CCNGG 7 cut(s) 154, 588, 638, 833, 857, 1446, 1914
StyI CCWWGG 2 cut(s) 2212, 2501
TaaI ACNGT 4 cut(s) 214, 615, 1466, 2470
TaiI ACGT 3 cut(s) 815, 878, 1009
TaqII GACCGA 1 cut(s) 2012
TasI AATT 8 cut(s) 344, 1057, 1138, 1437, 1691, 1713, 1932, 1979
TatI WGTACW 1 cut(s) 732
TauI GCSGC 7 cut(s) 636, 828, 1080, 1242, 1248, 1346, 1562
Tru1I TTAA 2 cut(s) 351, 1712
Tru9I TTAA 2 cut(s) 351, 1712
TscAI CASTG 3 cut(s) 848, 1471, 2473
TseFI GTSAC 2 cut(s) 1502, 2479
TseI GCWGC 5 cut(s) 1066, 1242, 1271, 1556, 2410
Tsp45I GTSAC 2 cut(s) 1502, 2479
TspGWI ACGGA 3 cut(s) 297, 1028, 1580
TspRI CASTG 3 cut(s) 848, 1471, 2473
VpaK11BI GGWCC 5 cut(s) 391, 862, 1424, 1945, 1984
XapI RAATTY 3 cut(s) 1057, 1437, 1691
XceI RCATGY 1 cut(s) 2314
XhoI CTCGAG 1 cut(s) 1893
XmaJI CCTAGG 2 cut(s) 2212, 2501
XmnI GAANNNNTTC 3 cut(s) 289, 316, 1437
XspI CTAG 9 cut(s) 101, 684, 1283, 1523, 1970, 2144, 2213, 2435, 2502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.