pycom07g01900

Belongs to the CTL (choline transporter-like) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002251
Physical Location & Seq
Reverse (-)
1493184 .. 1496500
3317 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1761 bp
ATGAACCCACCAGGCCCTCTCTCATTGGTCAAAGTCACACACGCGCCACTGGAGCACGTTAGCCATATCTATGCTACGACTGGGCGGGTGGAGGAGAGAGAAAGAGAGGAGCTTGAGGAGAGAGAGGACTATGGGAGGAAGGAGGAGGAGGAGGAGGATGTGAGAGATGTTGAGAAAGGGGAGGACGAGAGAGAAGGAGATGTTCATGTGTCAATGTTGCAGAGGCTGAATCCAACAAACCCTTTGAGAATTGTAATAAATAGCAAAACCAGAGCTGCAACTCCGCCGCCGCCGCCGCCGCCGTCTCGGTTCTCTAATATCAGAGGCGGTGGTGGTGGTCATGGTGGTGCTACTGCTACTCCTTCCCCTCCTCAGTTATCCTCTCACACCAACACTCCCCAGCATTCTCAGCCTCGTTCGACACCAAACCCACAACCATCTTTTACAACACTGAATTCAAGAAAATACACCAACAAGATATCTCTGTTTCTGTTTCTTCTGCACATGGTTGCAGCCATTGGGCTAGTCGGTTTTCTTCTGTTCAGGGGAGTTCAAGGGCTGATAGAAGCGTCAGACAACAAGGTCAAAAGAGCCGAGAAACGAGTGTTGAAGTTTTTTCTACCACAAGTCGAAGCTGCATCTCTTTTAAGCATTGTTCTTGCATTTGCATGGCAAAAGGCAGTCAGGCTATGGCCTAAGTTTTTTATTCATTTCATACTATGGACCACATTTGTCATGTCTCTGTCTGCTGGAATTCTGCTAATTTGCTTCCAAAAGGCTCCCACTGATGGCGTTGCATTCTGCTTTATTGCATTTGCGATAGGCAATGGCTTATACGCTTGTTGGATCACACAGCGGATAGGATTTTGTAGCAAGATATTGATCAAATCGCTCGAACCCGTATCAAAGTTCCCCGATTTGAATCAACCCACTTATTGGATGCTTGGGGTCGGATTTCTGTGGATGTCCCTGTGGATTCTAGCTGTGATTGGAGCCTTGAATTTCTACTTTCCTCCACTGATCATAATTTTATTGGTCTTGAGCTTAGCTTGGACAGCTGAAGTAATGAGGAACGTTGTAAATATGACCGTCAGTAGGGTAATTTCTCTTTATTATCTCAGAGGAATGCAATCCAATACTCAATTTTGCTTCCAGAGAGCCTTGAGTAAAAATCTTGGAAGTGCTTGTTTGGGTTCACTCTTTGTACCTGCAATTGAAGCACTGAGAATTGTTGCTCGGGCTTTAAATTTACTCAAGGGAGAAGATGAGTTCATGTTTTCTTGTGCACATTGCTGTCTCAAAGTCATGGAGTGCATCTTCAGATATGGCAACGGCTGGGCCTTCATACAGATAGCAGTGTATGGGAAAGGTTTTGTGAGGGCATCACAAGACACCTGGGAGCTCTTTGAACGGCAAGAAATGGAACCAATTGTTGATTCCGACATTACCAGCTCAATCTGCTTCCTAACCGGCGTCTGCAGCGGCTCTATCTGTGTCATAGTTGTGGCGGCTTGGACCGCTAGAGTGCACCAAAGTTTCACAGCCACCATTTCCCTCCTTACATTCTTCATTGGATACCTTATTACGAGGATAGCCATGGCACTACCACATGCTTGTGTAGGTAGTTACTTTGTTTGCTATGCAGAGAATCCAGACAACAGATTGTTTGACAACACAATCAAGGATCGCCTCGCTTTGATGAAATCTGGTCGAGATGTAGTCGTTCCCACACCTCGAGTCCCCAGCCGGTTTGCAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Choline_transpo

Protein Analysis

587

Amino Acids

65.52

Weight (kDa)

8.88

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Choline_transpo PF04515 268 - 552 1.2e-42 Plasma-membrane choline transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
Acc36I ACCTGC 1 cut(s) 1216
AccB7I CCANNNNNTGG 1 cut(s) 936
AccII CGCG 1 cut(s) 44
AclI AACGTT 1 cut(s) 1074
AclWI GGATC 2 cut(s) 854, 1692
AcsI RAATTY 4 cut(s) 454, 753, 1000, 1246
AcuI CTGAAG 2 cut(s) 1080, 1303
AcyI GRCGYC 1 cut(s) 1473
AfaI GTAC 1 cut(s) 1206
AfiI CCNNNNNNNGG 3 cut(s) 788, 936, 1095
AgsI TTSAA 7 cut(s) 459, 554, 610, 922, 1000, 1217, 1409
AjnI CCWGG 2 cut(s) 10, 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 1420, 1452
AluBI AGCT 9 cut(s) 112, 275, 635, 983, 1044, 1049, 1058, 1402, 1452
AluI AGCT 9 cut(s) 112, 275, 635, 983, 1044, 1049, 1058, 1402, 1452
Alw21I GWGCWC 4 cut(s) 57, 1288, 1404, 1530
Alw26I GTCTC 3 cut(s) 309, 744, 1301
Alw44I GTGCAC 2 cut(s) 1284, 1526
AlwI GGATC 2 cut(s) 854, 1692
AlwNI CAGNNNCTG 1 cut(s) 226
Ama87I CYCGRG 2 cut(s) 1236, 1734
AoxI GGCC 3 cut(s) 13, 692, 1338
ApaLI GTGCAC 2 cut(s) 1284, 1526
ApeKI GCWGC 4 cut(s) 275, 512, 635, 1479
ApoI RAATTY 4 cut(s) 454, 753, 1000, 1246
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 4 cut(s) 14, 723, 1338, 1515
AvaI CYCGRG 2 cut(s) 1236, 1734
AvaII GGWCC 2 cut(s) 723, 1515
BaeGI GKGCMC 2 cut(s) 1288, 1530
BanII GRGCYC 1 cut(s) 1404
Bbv12I GWGCWC 4 cut(s) 57, 1288, 1404, 1530
BbvI GCAGC 4 cut(s) 262, 524, 622, 1491
BccI CCATC 2 cut(s) 445, 782
BceAI ACGGC 3 cut(s) 286, 1348, 1427
BciT130I CCWGG 2 cut(s) 12, 1396
BciVI GTATCC 1 cut(s) 1568
BclI TGATCA 2 cut(s) 882, 1020
BcoDI GTCTC 3 cut(s) 309, 744, 1301
BfaI CTAG 3 cut(s) 524, 980, 1521
BfmI CTRYAG 1 cut(s) 1477
BfuAI ACCTGC 1 cut(s) 1216
BfuI GTATCC 1 cut(s) 1568
BlpI GCTNAGC 1 cut(s) 1045
Bme1390I CCNGG 2 cut(s) 12, 1396
Bme18I GGWCC 2 cut(s) 723, 1515
BmeT110I CYCGRG 2 cut(s) 1236, 1734
BmgT120I GGNCC 4 cut(s) 14, 723, 1338, 1515
BmiI GGNNCC 3 cut(s) 780, 994, 1425
BmrFI CCNGG 2 cut(s) 12, 1396
BmrI ACTGGG 1 cut(s) 90
BmsI GCATC 4 cut(s) 647, 930, 1323, 1391
BmuI ACTGGG 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 71
Bpu1102I GCTNAGC 1 cut(s) 1045
BpuEI CTTGAG 4 cut(s) 134, 1060, 1183, 1238
BsaBI GATNNNNATC 2 cut(s) 881, 921
BsaHI GRCGYC 1 cut(s) 1473
BsaJI CCNNGG 2 cut(s) 1395, 1596
BsaXI ACNNNNNCTCC 4 cut(s) 343, 373, 379, 409
Bsc4I CCNNNNNNNGG 3 cut(s) 788, 936, 1095
Bse118I RCCGGY 2 cut(s) 1469, 1746
Bse1I ACTGG 2 cut(s) 54, 85
Bse3DI GCAATG 2 cut(s) 832, 1288
Bse8I GATNNNNATC 2 cut(s) 881, 921
BseBI CCWGG 2 cut(s) 12, 1396
BseDI CCNNGG 2 cut(s) 1395, 1596
BseGI GGATG 3 cut(s) 163, 945, 969
BseJI GATNNNNATC 2 cut(s) 881, 921
BseLI CCNNNNNNNGG 3 cut(s) 788, 936, 1095
BseMI GCAATG 2 cut(s) 832, 1288
BseMII CTCAG 4 cut(s) 386, 422, 1132, 1214
BseNI ACTGG 2 cut(s) 54, 85
BseRI GAGGAG 8 cut(s) 107, 122, 131, 158, 161, 164, 167, 360
BseSI GKGCMC 2 cut(s) 1288, 1530
BseXI GCAGC 4 cut(s) 262, 524, 622, 1491
BseYI CCCAGC 3 cut(s) 399, 1335, 1742
BsgI GTGCAG 1 cut(s) 485
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 3 cut(s) 15, 694, 1340
BsiHKAI GWGCWC 4 cut(s) 57, 1288, 1404, 1530
BsiHKCI CYCGRG 2 cut(s) 1236, 1734
BsiSI CCGG 2 cut(s) 1470, 1747
BslFI GGGAC 2 cut(s) 952, 1724
BslI CCNNNNNNNGG 3 cut(s) 788, 936, 1095
BsmAI GTCTC 3 cut(s) 309, 744, 1301
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 2 cut(s) 952, 1724
BsmI GAATGC 3 cut(s) 403, 797, 1131
BsnI GGCC 3 cut(s) 15, 694, 1340
BsoBI CYCGRG 2 cut(s) 1236, 1734
Bsp1286I GDGCHC 4 cut(s) 57, 1288, 1404, 1530
Bsp143I GATC 4 cut(s) 846, 882, 1020, 1684
Bsp1720I GCTNAGC 1 cut(s) 1045
Bsp19I CCATGG 1 cut(s) 1596
BspANI GGCC 3 cut(s) 15, 694, 1340
BspCNI CTCAG 4 cut(s) 385, 421, 1131, 1215
BspFNI CGCG 1 cut(s) 44
BspLI GGNNCC 3 cut(s) 780, 994, 1425
BspMAI CTGCAG 1 cut(s) 1481
BspMI ACCTGC 1 cut(s) 1216
BspPI GGATC 2 cut(s) 854, 1692
BsrDI GCAATG 2 cut(s) 832, 1288
BsrFI RCCGGY 2 cut(s) 1469, 1746
BsrI ACTGG 2 cut(s) 54, 85
BssAI RCCGGY 2 cut(s) 1469, 1746
BssECI CCNNGG 2 cut(s) 1395, 1596
BssMI GATC 4 cut(s) 846, 882, 1020, 1684
BssNI GRCGYC 1 cut(s) 1473
BssT1I CCWWGG 1 cut(s) 1596
Bst2UI CCWGG 2 cut(s) 12, 1396
Bst4CI ACNGT 1 cut(s) 1090
BstACI GRCGYC 1 cut(s) 1473
BstDEI CTNAG 6 cut(s) 372, 408, 696, 1045, 1118, 1223
BstDSI CCRYGG 1 cut(s) 1596
BstF5I GGATG 3 cut(s) 163, 945, 969
BstFNI CGCG 1 cut(s) 44
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 4 cut(s) 849, 885, 1023, 1687
BstMAI GTCTC 3 cut(s) 309, 744, 1301
BstMBI GATC 4 cut(s) 846, 882, 1020, 1684
BstNI CCWGG 2 cut(s) 12, 1396
BstNSI RCATGY 1 cut(s) 1613
BstSCI CCNGG 2 cut(s) 10, 1394
BstSFI CTRYAG 1 cut(s) 1477
BstSLI GKGCMC 2 cut(s) 1288, 1530
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 4 cut(s) 262, 524, 622, 1491
BsuI GTATCC 1 cut(s) 1568
BsuRI GGCC 3 cut(s) 15, 694, 1340
BtgI CCRYGG 1 cut(s) 1596
BtsCI GGATG 3 cut(s) 163, 945, 969
BtsI GCAGTG 1 cut(s) 1362
BtsIMutI CAGTG 6 cut(s) 47, 449, 783, 1016, 1220, 1362
BveI ACCTGC 1 cut(s) 1216
CaiI CAGNNNCTG 1 cut(s) 226
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 2 cut(s) 1469, 1746
Cfr13I GGNCC 4 cut(s) 14, 723, 1338, 1515
CseI GACGC 2 cut(s) 558, 1462
Csp6I GTAC 1 cut(s) 1205
CviAII CATG 9 cut(s) 206, 341, 505, 669, 736, 1273, 1306, 1597, 1610
CviQI GTAC 1 cut(s) 1205
DdeI CTNAG 6 cut(s) 372, 408, 696, 1045, 1118, 1223
DpnI GATC 4 cut(s) 848, 884, 1022, 1686
DpnII GATC 4 cut(s) 846, 882, 1020, 1684
DraI TTTAAA 1 cut(s) 1245
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
EciI GGCGGA 1 cut(s) 273
Ecl136II GAGCTC 1 cut(s) 1402
Eco130I CCWWGG 1 cut(s) 1596
Eco24I GRGCYC 1 cut(s) 1404
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 2 cut(s) 723, 1515
Eco53kI GAGCTC 1 cut(s) 1402
Eco57I CTGAAG 2 cut(s) 1080, 1303
Eco88I CYCGRG 2 cut(s) 1236, 1734
EcoICRI GAGCTC 1 cut(s) 1402
EcoO109I RGGNCCY 1 cut(s) 14
EcoRI GAATTC 2 cut(s) 454, 753
EcoRII CCWGG 2 cut(s) 10, 1394
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 1596
EcoT38I GRGCYC 1 cut(s) 1404
ErhI CCWWGG 1 cut(s) 1596
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 9 cut(s) 209, 344, 508, 672, 739, 1276, 1309, 1600, 1613
FaqI GGGAC 2 cut(s) 952, 1724
FatI CATG 9 cut(s) 205, 340, 504, 668, 735, 1272, 1305, 1596, 1609
FauI CCCGC 1 cut(s) 78
FbaI TGATCA 2 cut(s) 882, 1020
FokI GGATG 3 cut(s) 170, 952, 976
FriOI GRGCYC 1 cut(s) 1404
FspBI CTAG 3 cut(s) 524, 980, 1521
GlaI GCGC 1 cut(s) 45
GsaI CCCAGC 3 cut(s) 403, 1339, 1746
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 3 cut(s) 15, 694, 1340
HapII CCGG 2 cut(s) 1470, 1747
HgaI GACGC 2 cut(s) 558, 1462
HhaI GCGC 1 cut(s) 46
Hin1I GRCGYC 1 cut(s) 1473
Hin1II CATG 9 cut(s) 209, 344, 508, 672, 739, 1276, 1309, 1600, 1613
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HinfI GANTC 6 cut(s) 229, 922, 976, 1436, 1648, 1737
HpaII CCGG 2 cut(s) 1470, 1747
Hpy166II GTNNAC 3 cut(s) 1196, 1286, 1528
Hpy188I TCNGA 6 cut(s) 323, 574, 953, 1121, 1322, 1441
Hpy188III TCNNGA 5 cut(s) 459, 1039, 1153, 1652, 1712
Hpy8I GTNNAC 3 cut(s) 1196, 1286, 1528
HpyAV CCTTC 4 cut(s) 133, 188, 372, 1351
HpyCH4III ACNGT 1 cut(s) 1090
HpyCH4IV ACGT 2 cut(s) 57, 1074
HpyF3I CTNAG 6 cut(s) 372, 408, 696, 1045, 1118, 1223
HpySE526I ACGT 2 cut(s) 57, 1074
Hsp92I GRCGYC 1 cut(s) 1473
Hsp92II CATG 9 cut(s) 209, 344, 508, 672, 739, 1276, 1309, 1600, 1613
HspAI GCGC 1 cut(s) 44
Ksp22I TGATCA 2 cut(s) 882, 1020
Kzo9I GATC 4 cut(s) 846, 882, 1020, 1684
LmnI GCTCC 5 cut(s) 52, 109, 784, 992, 1399
Lsp1109I GCAGC 4 cut(s) 262, 524, 622, 1491
LweI GCATC 4 cut(s) 647, 930, 1323, 1391
MaeI CTAG 3 cut(s) 524, 980, 1521
MaeII ACGT 2 cut(s) 57, 1074
MaeIII GTNAC 2 cut(s) 34, 1625
MalI GATC 4 cut(s) 848, 884, 1022, 1686
MboI GATC 4 cut(s) 846, 882, 1020, 1684
MboII GAAGA 5 cut(s) 488, 527, 1274, 1309, 1558
MfeI CAATTG 2 cut(s) 1212, 1428
MhlI GDGCHC 4 cut(s) 57, 1288, 1404, 1530
MlyI GAGTC 1 cut(s) 1746
MmeI TCCRAC 4 cut(s) 257, 824, 931, 1464
MseI TTAA 2 cut(s) 647, 1244
MslI CAYNNNNRTG 5 cut(s) 69, 345, 667, 1502, 1614
MspA1I CMGCKG 3 cut(s) 856, 1058, 1482
MspI CCGG 2 cut(s) 1470, 1747
MspR9I CCNGG 2 cut(s) 12, 1396
MunI CAATTG 2 cut(s) 1212, 1428
Mva1269I GAATGC 3 cut(s) 403, 797, 1131
MvaI CCWGG 2 cut(s) 12, 1396
MvnI CGCG 1 cut(s) 44
NcoI CCATGG 1 cut(s) 1596
NdeII GATC 4 cut(s) 846, 882, 1020, 1684
NlaIII CATG 9 cut(s) 209, 344, 508, 672, 739, 1276, 1309, 1600, 1613
NlaIV GGNNCC 3 cut(s) 780, 994, 1425
NmeAIII GCCGAG 1 cut(s) 619
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 1613
PaeR7I CTCGAG 1 cut(s) 1734
PctI GAATGC 3 cut(s) 403, 797, 1131
PfeI GAWTC 5 cut(s) 229, 922, 976, 1436, 1648
PflMI CCANNNNNTGG 1 cut(s) 936
PleI GAGTC 1 cut(s) 1745
PpsI GAGTC 1 cut(s) 1745
Psp124BI GAGCTC 1 cut(s) 1404
Psp1406I AACGTT 1 cut(s) 1074
Psp6I CCWGG 2 cut(s) 10, 1394
PspFI CCCAGC 3 cut(s) 399, 1335, 1742
PspGI CCWGG 2 cut(s) 10, 1394
PspN4I GGNNCC 3 cut(s) 780, 994, 1425
PspPI GGNCC 4 cut(s) 14, 723, 1338, 1515
PspXI VCTCGAGB 1 cut(s) 1734
PstI CTGCAG 1 cut(s) 1481
PstNI CAGNNNCTG 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 1 cut(s) 1206
RsaNI GTAC 1 cut(s) 1205
RseI CAYNNNNRTG 5 cut(s) 69, 345, 667, 1502, 1614
SacI GAGCTC 1 cut(s) 1404
SaqAI TTAA 2 cut(s) 647, 1244
Sau3AI GATC 4 cut(s) 846, 882, 1020, 1684
Sau96I GGNCC 4 cut(s) 14, 723, 1338, 1515
SchI GAGTC 1 cut(s) 1746
ScrFI CCNGG 2 cut(s) 12, 1396
SduI GDGCHC 4 cut(s) 57, 1288, 1404, 1530
SfaNI GCATC 4 cut(s) 647, 930, 1323, 1391
SfcI CTRYAG 1 cut(s) 1477
Sfr274I CTCGAG 1 cut(s) 1734
SinI GGWCC 2 cut(s) 723, 1515
SlaI CTCGAG 1 cut(s) 1734
SmiMI CAYNNNNRTG 5 cut(s) 69, 345, 667, 1502, 1614
SmlI CTYRAG 5 cut(s) 113, 1039, 1162, 1253, 1734
SmoI CTYRAG 5 cut(s) 113, 1039, 1162, 1253, 1734
SspMI CTAG 3 cut(s) 524, 980, 1521
SstI GAGCTC 1 cut(s) 1404
StyD4I CCNGG 2 cut(s) 10, 1394
StyI CCWWGG 1 cut(s) 1596
TaaI ACNGT 1 cut(s) 1090
TaiI ACGT 2 cut(s) 60, 1077
TaqI TCGA 5 cut(s) 419, 630, 894, 1711, 1735
TauI GCSGC 7 cut(s) 289, 292, 295, 298, 301, 1485, 1511
TfiI GAWTC 5 cut(s) 229, 922, 976, 1436, 1648
Tru1I TTAA 2 cut(s) 647, 1244
Tru9I TTAA 2 cut(s) 647, 1244
TscAI CASTG 6 cut(s) 54, 456, 790, 1023, 1227, 1362
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 4 cut(s) 275, 512, 635, 1479
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 8 cut(s) 17, 194, 698, 703, 1261, 1333, 1558, 1715
TspRI CASTG 6 cut(s) 54, 456, 790, 1023, 1227, 1362
Van91I CCANNNNNTGG 1 cut(s) 936
VneI GTGCAC 2 cut(s) 1284, 1526
VpaK11BI GGWCC 2 cut(s) 723, 1515
XapI RAATTY 4 cut(s) 454, 753, 1000, 1246
XceI RCATGY 1 cut(s) 1613
XhoI CTCGAG 1 cut(s) 1734
XspI CTAG 3 cut(s) 524, 980, 1521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.