pycom10g07650

B3 domain-containing protein Os01g0234100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001540
Physical Location & Seq
Reverse (-)
9278773 .. 9281598
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1308 bp
ATGGTGGAAGCAATGGCTATTCTTCAAGACCAGAAACAGAGCTCCACTAACCCACTGCTACTGCTACCGCCGCCGCCTCCACCGCATAAGGGTATGGCAACCGAAAAGCCGTTTGGGCAAAAACCTCCATGCCTCCAACTGAAGACTACAAACGTGTTGAATGCTAAAACTAATCCCATTATATTGGAATGTCGTAAACGAAAGACCCCGGTAAGAGTTACAGTAGAAAGCTTATATGAGAGCTCCCAAGCTCAGTCTTCTGTTATGGAAAGAGCAAAGAAGATCGAAGCAAGCCTAAACCCTAGGTTTCCTTCACTACTCAAAGTTATGATGCCGTCACAAGTTTCGGGAGGATTTTGCCTCAATCTTGCTACGAAATTTCGCAGGGAACATATGCCAAAACAAGATTCAATGATTGTTTTGGAAGATGAAAATGGGAGAGAATTTGAAACAAAATATCTTGTGGAGAAGGGTGGACTGAGTGGTGGATGGAGAGGGTTTTCAATTGCTCACAAGATAATGAAGGGAGATGCTGTGATTTTCCATCTGGTCACTCCTTTCAAATTTAAGGTGTATATCATTAGATCAAATGAAGTGGACTGTAATTTTCCCCTTATGAGGGTAGATCCTCCCATCAAACAGGTGGATACGGGAGGCGTTACTGCACGTGGAAAGGAACGTGAGATTTTGGAACCTATTCCGCAGGAGAATGCAATAGTCTGTTATACAGACTCTGGTCCAATATCAGATCAGTCTGAAAATGATTGTGTAGATGTTGGTTCTGAATTTCTGGATGGTCTTAGACTTTCAGAGTCAGTTGTCACTTTCAAAGAAGTGAACTGTTTGGAGAACTTCAACGTTCAGGTCAATGGTTTAATCATAAACTCTGAATTTTCGAAATACGTCATGACCAAGTATTATGAGCTTTGCTGCAGTCAGAACTCCTTCCTTCATGGACAACTTCTCGAGGGTCTGAACTGTAAACTGATTGCAGGAGTAATTTCTGAGACAGTCAACATTGCAGATGCCATCAGGGCCTGCAAGATCACTACCCCGGAAGATTATCTTTCGACCTGGGATAAGACTCTGAAAGCCTTTGATGATTTAGGCATGAATGTTAGGTTCCTGCGTGCCCGCCTGGACCAGCTTGCAAGTTTTGCTTCAAAATCAAGAAGACCTAAGGAGGCAAATGTTTTGGAAGTGAAAGAGGCCATGGATAGTCGTCTTGACTCTGAGATTGAGACTCCGAATAGGAGTTCAGAGGAGCAGGAAATTATGTTCCAAGAGGTGGCTAAAGCCCCTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

436

Amino Acids

48.85

Weight (kDa)

6.1

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 107 - 196 5.7e-14 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1288
AciI CCGC 6 cut(s) 68, 71, 74, 83, 701, 1135
AclI AACGTT 1 cut(s) 858
AclWI GGATC 1 cut(s) 620
AcsI RAATTY 5 cut(s) 377, 443, 563, 785, 890
AcuI CTGAAG 1 cut(s) 161
AcvI CACGTG 1 cut(s) 668
AfiI CCNNNNNNNGG 4 cut(s) 89, 618, 619, 1288
AflIII ACRYGT 1 cut(s) 153
AgsI TTSAA 9 cut(s) 26, 160, 411, 449, 504, 562, 829, 856, 1164
AjnI CCWGG 2 cut(s) 1073, 1137
AjuI GAANNNNNNNTTGG 4 cut(s) 96, 128, 391, 423
AloI GAACNNNNNNTCC 2 cut(s) 1262, 1294
AluBI AGCT 6 cut(s) 42, 231, 243, 251, 925, 1147
AluI AGCT 6 cut(s) 42, 231, 243, 251, 925, 1147
Alw21I GWGCWC 2 cut(s) 44, 245
Alw26I GTCTC 2 cut(s) 1001, 1235
AlwI GGATC 1 cut(s) 620
AlwNI CAGNNNCTG 2 cut(s) 734, 1038
Ama87I CYCGRG 1 cut(s) 965
AoxI GGCC 2 cut(s) 1035, 1209
ApeKI GCWGC 1 cut(s) 930
ApoI RAATTY 5 cut(s) 377, 443, 563, 785, 890
Asp700I GAANNNNTTC 2 cut(s) 696, 944
AspA2I CCTAGG 1 cut(s) 302
AspS9I GGNCC 3 cut(s) 737, 1035, 1141
AsuC2I CCSGG 2 cut(s) 209, 1055
AsuII TTCGAA 1 cut(s) 896
AvaI CYCGRG 1 cut(s) 965
AvaII GGWCC 2 cut(s) 737, 1141
AvrII CCTAGG 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 1179
BaeGI GKGCMC 1 cut(s) 1135
BanII GRGCYC 2 cut(s) 44, 245
BbrPI CACGTG 1 cut(s) 668
BbsI GAAGAC 3 cut(s) 149, 249, 1180
Bbv12I GWGCWC 2 cut(s) 44, 245
BbvI GCAGC 1 cut(s) 917
BccI CCATC 5 cut(s) 483, 552, 641, 788, 1037
BceAI ACGGC 2 cut(s) 94, 319
BciT130I CCWGG 2 cut(s) 1075, 1139
BciVI GTATCC 1 cut(s) 640
BcnI CCSGG 2 cut(s) 209, 1055
BcoDI GTCTC 2 cut(s) 1001, 1235
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 931
BfuI GTATCC 1 cut(s) 640
BglI GCCNNNNNGGC 2 cut(s) 115, 1034
BisI GCNGC 3 cut(s) 71, 74, 931
BlnI CCTAGG 1 cut(s) 302
BlsI GCNGC 3 cut(s) 72, 75, 932
Bme1390I CCNGG 4 cut(s) 209, 1055, 1075, 1139
Bme18I GGWCC 2 cut(s) 737, 1141
BmeT110I CYCGRG 1 cut(s) 965
BmgT120I GGNCC 3 cut(s) 737, 1035, 1141
BmiI GGNNCC 2 cut(s) 693, 1124
BmrFI CCNGG 4 cut(s) 209, 1055, 1075, 1139
BmsI GCATC 3 cut(s) 321, 520, 1015
BoxI GACNNNNGTC 1 cut(s) 735
BpiI GAAGAC 3 cut(s) 149, 249, 1180
Bpu14I TTCGAA 1 cut(s) 896
BpuMI CCSGG 2 cut(s) 209, 1055
BsaAI YACGTR 1 cut(s) 668
BsaJI CCNNGG 6 cut(s) 207, 302, 1053, 1074, 1212, 1301
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 4 cut(s) 89, 618, 619, 1288
Bse21I CCTNAGG 1 cut(s) 1179
Bse3DI GCAATG 2 cut(s) 18, 1017
BseBI CCWGG 2 cut(s) 1075, 1139
BseDI CCNNGG 6 cut(s) 207, 302, 1053, 1074, 1212, 1301
BseGI GGATG 2 cut(s) 494, 799
BseLI CCNNNNNNNGG 4 cut(s) 89, 618, 619, 1288
BseMI GCAATG 2 cut(s) 18, 1017
BseMII CTCAG 4 cut(s) 266, 470, 996, 1224
BseRI GAGGAG 1 cut(s) 1277
BseSI GKGCMC 1 cut(s) 1135
BseXI GCAGC 1 cut(s) 917
BsgI GTGCAG 1 cut(s) 648
BshFI GGCC 2 cut(s) 1037, 1211
BsiHKAI GWGCWC 2 cut(s) 44, 245
BsiHKCI CYCGRG 1 cut(s) 965
BsiSI CCGG 2 cut(s) 209, 1055
BslI CCNNNNNNNGG 4 cut(s) 89, 618, 619, 1288
BsmAI GTCTC 2 cut(s) 1001, 1235
BsmI GAATGC 2 cut(s) 166, 715
BsnI GGCC 2 cut(s) 1037, 1211
BsoBI CYCGRG 1 cut(s) 965
Bsp119I TTCGAA 1 cut(s) 896
Bsp1286I GDGCHC 3 cut(s) 44, 245, 1135
Bsp143I GATC 5 cut(s) 282, 584, 625, 748, 1044
Bsp19I CCATGG 1 cut(s) 1212
BspACI CCGC 6 cut(s) 68, 71, 74, 83, 701, 1135
BspANI GGCC 2 cut(s) 1037, 1211
BspCNI CTCAG 4 cut(s) 265, 471, 997, 1225
BspHI TCATGA 1 cut(s) 906
BspLI GGNNCC 2 cut(s) 693, 1124
BspMAI CTGCAG 1 cut(s) 935
BspPI GGATC 1 cut(s) 620
BspT104I TTCGAA 1 cut(s) 896
BsrDI GCAATG 2 cut(s) 18, 1017
BssECI CCNNGG 6 cut(s) 207, 302, 1053, 1074, 1212, 1301
BssMI GATC 5 cut(s) 282, 584, 625, 748, 1044
BssT1I CCWWGG 3 cut(s) 302, 1212, 1301
Bst2UI CCWGG 2 cut(s) 1075, 1139
Bst4CI ACNGT 5 cut(s) 223, 602, 842, 980, 1012
BstAPI GCANNNNNTGC 1 cut(s) 1157
BstBAI YACGTR 1 cut(s) 668
BstBI TTCGAA 1 cut(s) 896
BstC8I GCNNGC 5 cut(s) 292, 1039, 1131, 1135, 1149
BstDEI CTNAG 6 cut(s) 252, 479, 800, 1005, 1179, 1233
BstDSI CCRYGG 1 cut(s) 1212
BstF5I GGATG 2 cut(s) 494, 799
BstKTI GATC 5 cut(s) 285, 587, 628, 751, 1047
BstMAI GTCTC 2 cut(s) 1001, 1235
BstMBI GATC 5 cut(s) 282, 584, 625, 748, 1044
BstMWI GCNNNNNNNGC 5 cut(s) 70, 82, 115, 1034, 1157
BstNI CCWGG 2 cut(s) 1075, 1139
BstPAI GACNNNNGTC 1 cut(s) 735
BstSCI CCNGG 4 cut(s) 207, 1053, 1073, 1137
BstSFI CTRYAG 1 cut(s) 931
BstSLI GKGCMC 1 cut(s) 1135
BstV1I GCAGC 1 cut(s) 917
BstV2I GAAGAC 3 cut(s) 149, 249, 1180
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 184
BstYI RGATCY 1 cut(s) 625
Bsu36I CCTNAGG 1 cut(s) 1179
BsuI GTATCC 1 cut(s) 640
BsuRI GGCC 2 cut(s) 1037, 1211
BtgI CCRYGG 1 cut(s) 1212
BtsCI GGATG 2 cut(s) 494, 799
BtsI GCAGTG 1 cut(s) 53
BtsIMutI CAGTG 1 cut(s) 53
Cac8I GCNNGC 5 cut(s) 292, 1039, 1131, 1135, 1149
CaiI CAGNNNCTG 2 cut(s) 734, 1038
CciI TCATGA 1 cut(s) 906
Cfr13I GGNCC 3 cut(s) 737, 1035, 1141
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 5 cut(s) 129, 907, 953, 1111, 1213
DdeI CTNAG 6 cut(s) 252, 479, 800, 1005, 1179, 1233
DpnI GATC 5 cut(s) 284, 586, 627, 750, 1046
DpnII GATC 5 cut(s) 282, 584, 625, 748, 1044
Ecl136II GAGCTC 2 cut(s) 42, 243
Eco130I CCWWGG 3 cut(s) 302, 1212, 1301
Eco24I GRGCYC 2 cut(s) 44, 245
Eco47I GGWCC 2 cut(s) 737, 1141
Eco53kI GAGCTC 2 cut(s) 42, 243
Eco57I CTGAAG 1 cut(s) 161
Eco72I CACGTG 1 cut(s) 668
Eco81I CCTNAGG 1 cut(s) 1179
Eco88I CYCGRG 1 cut(s) 965
EcoICRI GAGCTC 2 cut(s) 42, 243
EcoO109I RGGNCCY 1 cut(s) 1035
EcoRII CCWGG 2 cut(s) 1073, 1137
EcoT14I CCWWGG 3 cut(s) 302, 1212, 1301
EcoT38I GRGCYC 2 cut(s) 44, 245
ErhI CCWWGG 3 cut(s) 302, 1212, 1301
FaeI CATG 5 cut(s) 132, 910, 956, 1114, 1216
FalI AAGNNNNNCTT 4 cut(s) 1050, 1082, 1144, 1176
FatI CATG 5 cut(s) 128, 906, 952, 1110, 1212
FauI CCCGC 1 cut(s) 1142
FauNDI CATATG 1 cut(s) 393
Fnu4HI GCNGC 3 cut(s) 71, 74, 931
FokI GGATG 2 cut(s) 501, 806
FriOI GRGCYC 2 cut(s) 44, 245
Fsp4HI GCNGC 3 cut(s) 71, 74, 931
FspBI CTAG 1 cut(s) 303
GluI GCNGC 3 cut(s) 71, 74, 931
HaeIII GGCC 2 cut(s) 1037, 1211
HapII CCGG 2 cut(s) 209, 1055
Hin1II CATG 5 cut(s) 132, 910, 956, 1114, 1216
HincII GTYRAC 1 cut(s) 1015
HindII GTYRAC 1 cut(s) 1015
HindIII AAGCTT 1 cut(s) 229
HinfI GANTC 6 cut(s) 407, 731, 812, 1084, 1229, 1243
HpaII CCGG 2 cut(s) 209, 1055
Hpy166II GTNNAC 6 cut(s) 197, 476, 598, 838, 983, 1015
Hpy188III TCNNGA 7 cut(s) 26, 348, 791, 907, 965, 1170, 1226
Hpy8I GTNNAC 6 cut(s) 197, 476, 598, 838, 983, 1015
HpyAV CCTTC 5 cut(s) 321, 463, 517, 955, 959
HpyCH4III ACNGT 5 cut(s) 223, 602, 842, 980, 1012
HpyCH4IV ACGT 5 cut(s) 153, 667, 679, 858, 903
HpyCH4V TGCA 7 cut(s) 665, 713, 933, 992, 1022, 1041, 1151
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 82, 115, 1034, 1157
HpyF3I CTNAG 6 cut(s) 252, 479, 800, 1005, 1179, 1233
HpySE526I ACGT 5 cut(s) 153, 667, 679, 858, 903
Hsp92II CATG 5 cut(s) 132, 910, 956, 1114, 1216
Kzo9I GATC 5 cut(s) 282, 584, 625, 748, 1044
LmnI GCTCC 3 cut(s) 47, 248, 1264
Lsp1109I GCAGC 1 cut(s) 917
LweI GCATC 3 cut(s) 321, 520, 1015
MaeI CTAG 1 cut(s) 303
MaeII ACGT 5 cut(s) 153, 667, 679, 858, 903
MaeIII GTNAC 5 cut(s) 217, 336, 550, 658, 820
MalI GATC 5 cut(s) 284, 586, 627, 750, 1046
MboI GATC 5 cut(s) 282, 584, 625, 748, 1044
MboII GAAGA 7 cut(s) 14, 154, 249, 292, 437, 1070, 1185
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 3 cut(s) 44, 245, 1135
MluCI AATT 9 cut(s) 377, 443, 504, 563, 604, 785, 890, 999, 1272
MlyI GAGTC 5 cut(s) 725, 821, 1078, 1223, 1237
MmeI TCCRAC 1 cut(s) 160
MroXI GAANNNNTTC 2 cut(s) 696, 944
MseI TTAA 2 cut(s) 567, 875
MspI CCGG 2 cut(s) 209, 1055
MspR9I CCNGG 4 cut(s) 209, 1055, 1075, 1139
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 2 cut(s) 166, 715
MvaI CCWGG 2 cut(s) 1075, 1139
MwoI GCNNNNNNNGC 5 cut(s) 70, 82, 115, 1034, 1157
NciI CCSGG 2 cut(s) 209, 1055
NcoI CCATGG 1 cut(s) 1212
NdeI CATATG 1 cut(s) 393
NdeII GATC 5 cut(s) 282, 584, 625, 748, 1044
NlaIII CATG 5 cut(s) 132, 910, 956, 1114, 1216
NlaIV GGNNCC 2 cut(s) 693, 1124
NmuCI GTSAC 3 cut(s) 336, 550, 820
NspV TTCGAA 1 cut(s) 896
PaeR7I CTCGAG 1 cut(s) 965
PagI TCATGA 1 cut(s) 906
PctI GAATGC 2 cut(s) 166, 715
PdmI GAANNNNTTC 2 cut(s) 696, 944
PfeI GAWTC 1 cut(s) 407
PflMI CCANNNNNTGG 1 cut(s) 1288
PkrI GCNGC 3 cut(s) 72, 75, 932
PleI GAGTC 5 cut(s) 725, 820, 1078, 1223, 1237
PmaCI CACGTG 1 cut(s) 668
PmlI CACGTG 1 cut(s) 668
PpsI GAGTC 5 cut(s) 725, 820, 1078, 1223, 1237
Ppu21I YACGTR 1 cut(s) 668
PshAI GACNNNNGTC 1 cut(s) 735
Psp124BI GAGCTC 2 cut(s) 44, 245
Psp1406I AACGTT 1 cut(s) 858
Psp6I CCWGG 2 cut(s) 1073, 1137
PspCI CACGTG 1 cut(s) 668
PspGI CCWGG 2 cut(s) 1073, 1137
PspN4I GGNNCC 2 cut(s) 693, 1124
PspPI GGNCC 3 cut(s) 737, 1035, 1141
PstI CTGCAG 1 cut(s) 935
PstNI CAGNNNCTG 2 cut(s) 734, 1038
PsuI RGATCY 1 cut(s) 625
SacI GAGCTC 2 cut(s) 44, 245
SaqAI TTAA 2 cut(s) 567, 875
SatI GCNGC 3 cut(s) 71, 74, 931
Sau3AI GATC 5 cut(s) 282, 584, 625, 748, 1044
Sau96I GGNCC 3 cut(s) 737, 1035, 1141
SchI GAGTC 5 cut(s) 725, 821, 1078, 1223, 1237
ScrFI CCNGG 4 cut(s) 209, 1055, 1075, 1139
SduI GDGCHC 3 cut(s) 44, 245, 1135
SfaNI GCATC 3 cut(s) 321, 520, 1015
SfcI CTRYAG 1 cut(s) 931
Sfr274I CTCGAG 1 cut(s) 965
SfuI TTCGAA 1 cut(s) 896
SinI GGWCC 2 cut(s) 737, 1141
SlaI CTCGAG 1 cut(s) 965
SmlI CTYRAG 1 cut(s) 965
SmoI CTYRAG 1 cut(s) 965
Sse9I AATT 9 cut(s) 377, 443, 504, 563, 604, 785, 890, 999, 1272
SsiI CCGC 6 cut(s) 68, 71, 74, 83, 701, 1135
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 2 cut(s) 44, 245
StyD4I CCNGG 4 cut(s) 207, 1053, 1073, 1137
StyI CCWWGG 3 cut(s) 302, 1212, 1301
TaaI ACNGT 5 cut(s) 223, 602, 842, 980, 1012
TaiI ACGT 5 cut(s) 156, 670, 682, 861, 906
TaqI TCGA 4 cut(s) 285, 896, 966, 1070
TasI AATT 9 cut(s) 377, 443, 504, 563, 604, 785, 890, 999, 1272
TauI GCSGC 2 cut(s) 73, 76
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 567, 875
Tru9I TTAA 2 cut(s) 567, 875
TscAI CASTG 1 cut(s) 60
TseFI GTSAC 3 cut(s) 336, 550, 820
TseI GCWGC 1 cut(s) 930
Tsp45I GTSAC 3 cut(s) 336, 550, 820
TspDTI ATGAA 5 cut(s) 444, 536, 606, 941, 1127
TspRI CASTG 1 cut(s) 60
Van91I CCANNNNNTGG 1 cut(s) 1288
VpaK11BI GGWCC 2 cut(s) 737, 1141
XapI RAATTY 5 cut(s) 377, 443, 563, 785, 890
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XhoI CTCGAG 1 cut(s) 965
XmaJI CCTAGG 1 cut(s) 302
XmnI GAANNNNTTC 2 cut(s) 696, 944
XspI CTAG 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.