pycom12g18680

Aminotransferase class-V

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002899
Physical Location & Seq
Forward (+)
20799094 .. 20800893
1800 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1461 bp
ATGACAGATTTACTCCTGGAGGTCATGGCGGAGAAGATTATCATGTTTTTCCGTTACTATGTTCCTCGATTCCTCTGCCCCAATCTTTCCTTCCGAATTTCGAGACCCTCTAATCGCCATTACCGTAACCTTAATCATAATGACACCCAAACCCAAGTCTCCGTTAAGCCGAAACCCTCTTCCTTCATCACAGACACCGAAATCCAATCTGAATTCTCCCACCATGCTCCCGGCATTGCCAGGATCAACAATGGCAGCTTTGGTTGCTGCCCTGCCTCCGTCATCTCTTCCTTGCACCAATGGCAGCTCAAATTGCTCCGCCAGCCCGACGACTTCTACTTGAACGAGCTTCCAAATAGGATACTTGAATCGAGAACAGTAATCAAAGATCTCATAAATGCAGAAGATGCGGAGGAAGTCTCCATTGTGGATAATGTCAGCACTGCTGTTGCTATAGTCCTGAAGCAGACGGCATGGTCTTTCGTGGAAGGGAAATTCGACAAAGGAGATGCTGTCATCATGTTTCGCTGCACTTATGGGGCGGTGAAGAACTCAATCAAGGCCTATTTTTTGCGCGCTGGTGGGTATGCGATCGAAGTTCCATTTAATTTTCCGGTGACTTCAAACGAGGAAATCGTGAGTGAATTCAAGACAGCTTTGAGGAGAGAAAAGGGTATTGGGAGGAGAGTGAGACTGGCGGTGATTGATCACGTTACTTGCATGCCAAGCTTTGTGATGCCGGTTAAGGAATTGGTTAAAATTTGCAAGGAAGAAGGCGTTGAGCAAGTTTTAGTTGATGCAGCTCATGCTGTTGGGTGTGTAGATGTGGACATGAACGAAATCACAGCGGATTTTTACACAGGTAATTTGTACAAGTGGTTTTTCTGCCCGCCTGCGGTTGCTTTCTTGTACTGCAGGAAATCAGTTATGACACATTTAGATTTGCATCATCCTGTTGTGTCTCATGACTATGGAAACGGATTGGTCAAAGAAACCGGTTGGGTTGGGACGAGGGATTATAGTCCCTATCTCGTTCTTCCTTCGGTTATGGAGTTTGTCAACAGATTCGAAGGCGGTGCAGAGGGAATCAGGAACCGGAATCATGATGCTGTTGTTGAGATGGGGAAGATGCTTGCAGAAGCTTGGGGAACCAATCTAGGGTGTCCACTGGAAATGTGTTCGAGCATGGTGATGGTTGGATTGCCGGCCTGTTTAGGGGTTCTGAGTGATGAAGATGCTATGAAGTTGTGGGAACTTCTGTGCAAGAAATTTGGTGTGGAAGTGAAAATACATTATCAAAACCCGAAAGATGGAGAGCTTGCACCTATAACTGGGTACGTTCGAATCTGCCACCAAATTTACAACAAATTTGAGGACTATTGCAGGCTCAGAGACGCAATCAACCAACTCGTTCGCGATGGCTTCAATTGCGGTAATCTTGAGATATGTGGGACCCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 map00270
Pfam Domains
Protein Families
Aminotran_5

Protein Analysis

487

Amino Acids

54.91

Weight (kDa)

6.13

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_5 PF00266 121 - 313 2.6e-13 Aminotransferase class-V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 475
AccII CGCG 2 cut(s) 576, 1416
AclWI GGATC 1 cut(s) 251
AcsI RAATTY 8 cut(s) 96, 212, 494, 644, 759, 1268, 1356, 1367
AcuI CTGAAG 1 cut(s) 482
AfaI GTAC 3 cut(s) 872, 911, 1337
AfiI CCNNNNNNNGG 5 cut(s) 895, 1158, 1215, 1310, 1331
AgeI ACCGGT 1 cut(s) 995
AgsI TTSAA 5 cut(s) 343, 368, 624, 649, 1426
AjnI CCWGG 2 cut(s) 15, 239
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 660, 692
AluBI AGCT 8 cut(s) 258, 307, 349, 656, 729, 803, 1142, 1318
AluI AGCT 8 cut(s) 258, 307, 349, 656, 729, 803, 1142, 1318
Alw26I GTCTC 6 cut(s) 97, 163, 424, 685, 966, 1386
AlwI GGATC 1 cut(s) 251
AoxI GGCC 2 cut(s) 561, 1206
ApeKI GCWGC 5 cut(s) 255, 267, 304, 528, 800
ApoI RAATTY 8 cut(s) 96, 212, 494, 644, 759, 1268, 1356, 1367
AsiGI ACCGGT 1 cut(s) 995
AspLEI GCGC 2 cut(s) 576, 578
AspS9I GGNCC 1 cut(s) 1452
AsuC2I CCSGG 1 cut(s) 231
AsuHPI GGTGA 4 cut(s) 556, 628, 712, 1201
AsuII TTCGAA 2 cut(s) 1068, 1342
AvaII GGWCC 1 cut(s) 1452
BarI GAAGNNNNNNTAC 2 cut(s) 1272, 1304
BbvI GCAGC 5 cut(s) 254, 267, 316, 515, 812
BccI CCATC 4 cut(s) 1114, 1186, 1304, 1412
BceAI ACGGC 1 cut(s) 486
BcgI CGANNNNNNTGC 4 cut(s) 57, 91, 1058, 1092
BciT130I CCWGG 2 cut(s) 17, 241
BciVI GTATCC 1 cut(s) 354
BclI TGATCA 1 cut(s) 706
BcnI CCSGG 1 cut(s) 231
BcoDI GTCTC 6 cut(s) 97, 163, 424, 685, 966, 1386
BfaI CTAG 2 cut(s) 1157, 1459
BfmI CTRYAG 2 cut(s) 453, 913
BfuI GTATCC 1 cut(s) 354
BglII AGATCT 1 cut(s) 388
BisI GCNGC 5 cut(s) 256, 268, 305, 529, 801
BlsI GCNGC 5 cut(s) 257, 269, 306, 530, 802
Bme1390I CCNGG 3 cut(s) 17, 231, 241
Bme18I GGWCC 1 cut(s) 1452
BmgT120I GGNCC 1 cut(s) 1452
BmiI GGNNCC 4 cut(s) 1094, 1150, 1453, 1454
BmrFI CCNGG 3 cut(s) 17, 231, 241
BmrI ACTGGG 1 cut(s) 1341
BmsI GCATC 8 cut(s) 397, 499, 726, 787, 955, 1096, 1119, 1225
BmuI ACTGGG 1 cut(s) 1341
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpmI CTGGAG 1 cut(s) 38
Bpu14I TTCGAA 2 cut(s) 1068, 1342
BpuEI CTTGAG 1 cut(s) 1460
BpuMI CCSGG 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 945
BsaI GGTCTC 1 cut(s) 97
BsaWI WCCGGW 3 cut(s) 613, 995, 1095
BsaXI ACNNNNNCTCC 2 cut(s) 498, 528
Bsc4I CCNNNNNNNGG 5 cut(s) 895, 1158, 1215, 1310, 1331
Bse118I RCCGGY 3 cut(s) 739, 995, 1204
Bse1I ACTGG 3 cut(s) 699, 1173, 1336
Bse3DI GCAATG 1 cut(s) 234
Bse8I GATNNNNATC 1 cut(s) 945
BseBI CCWGG 2 cut(s) 17, 241
BseGI GGATG 1 cut(s) 949
BseJI GATNNNNATC 1 cut(s) 945
BseLI CCNNNNNNNGG 5 cut(s) 895, 1158, 1215, 1310, 1331
BseMI GCAATG 1 cut(s) 234
BseMII CTCAG 2 cut(s) 1214, 1402
BseNI ACTGG 3 cut(s) 699, 1173, 1336
BsePI GCGCGC 1 cut(s) 574
BseRI GAGGAG 2 cut(s) 676, 697
BseXI GCAGC 5 cut(s) 254, 267, 316, 515, 812
BsgI GTGCAG 2 cut(s) 514, 1098
Bsh1236I CGCG 2 cut(s) 576, 1416
Bsh1285I CGRYCG 1 cut(s) 594
BshFI GGCC 2 cut(s) 563, 1208
BshTI ACCGGT 1 cut(s) 995
BsiEI CGRYCG 1 cut(s) 594
BsiSI CCGG 6 cut(s) 231, 614, 740, 996, 1096, 1205
BslFI GGGAC 2 cut(s) 1008, 1021
BslI CCNNNNNNNGG 5 cut(s) 895, 1158, 1215, 1310, 1331
BsmAI GTCTC 6 cut(s) 97, 163, 424, 685, 966, 1386
BsmBI CGTCTC 1 cut(s) 1386
BsmFI GGGAC 2 cut(s) 1008, 1021
BsnI GGCC 2 cut(s) 563, 1208
Bso31I GGTCTC 1 cut(s) 97
Bsp119I TTCGAA 2 cut(s) 1068, 1342
Bsp1407I TGTACA 1 cut(s) 870
Bsp143I GATC 4 cut(s) 243, 388, 591, 706
Bsp68I TCGCGA 1 cut(s) 1416
BspANI GGCC 2 cut(s) 563, 1208
BspCNI CTCAG 2 cut(s) 1215, 1401
BspFNI CGCG 2 cut(s) 576, 1416
BspHI TCATGA 2 cut(s) 964, 1102
BspLI GGNNCC 4 cut(s) 1094, 1150, 1453, 1454
BspMAI CTGCAG 1 cut(s) 917
BspPI GGATC 1 cut(s) 251
BspT104I TTCGAA 2 cut(s) 1068, 1342
BspTNI GGTCTC 1 cut(s) 97
BsrDI GCAATG 1 cut(s) 234
BsrFI RCCGGY 3 cut(s) 739, 995, 1204
BsrGI TGTACA 1 cut(s) 870
BsrI ACTGG 3 cut(s) 699, 1173, 1336
BssAI RCCGGY 3 cut(s) 739, 995, 1204
BssHII GCGCGC 1 cut(s) 574
BssMI GATC 4 cut(s) 243, 388, 591, 706
Bst2UI CCWGG 2 cut(s) 17, 241
Bst4CI ACNGT 2 cut(s) 125, 379
Bst6I CTCTTC 2 cut(s) 184, 292
BstAPI GCANNNNNTGC 2 cut(s) 407, 806
BstAUI TGTACA 1 cut(s) 870
BstBI TTCGAA 2 cut(s) 1068, 1342
BstC8I GCNNGC 9 cut(s) 323, 576, 722, 890, 894, 1134, 1206, 1320, 1385
BstDEI CTNAG 2 cut(s) 1223, 1388
BstF5I GGATG 1 cut(s) 949
BstFNI CGCG 2 cut(s) 576, 1416
BstHHI GCGC 2 cut(s) 576, 578
BstKTI GATC 4 cut(s) 246, 391, 594, 709
BstMAI GTCTC 6 cut(s) 97, 163, 424, 685, 966, 1386
BstMBI GATC 4 cut(s) 243, 388, 591, 706
BstMCI CGRYCG 1 cut(s) 594
BstMWI GCNNNNNNNGC 8 cut(s) 264, 301, 313, 322, 407, 726, 806, 1428
BstNI CCWGG 2 cut(s) 17, 241
BstNSI RCATGY 1 cut(s) 724
BstSCI CCNGG 3 cut(s) 15, 229, 239
BstSFI CTRYAG 2 cut(s) 453, 913
BstUI CGCG 2 cut(s) 576, 1416
BstV1I GCAGC 5 cut(s) 254, 267, 316, 515, 812
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuI GTATCC 1 cut(s) 354
BsuRI GGCC 2 cut(s) 563, 1208
BtgZI GCGATG 1 cut(s) 1431
BtsCI GGATG 1 cut(s) 949
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 2 cut(s) 441, 1166
BtuMI TCGCGA 1 cut(s) 1416
Cac8I GCNNGC 9 cut(s) 323, 576, 722, 890, 894, 1134, 1206, 1320, 1385
CciI TCATGA 2 cut(s) 964, 1102
CfoI GCGC 2 cut(s) 576, 578
Cfr10I RCCGGY 3 cut(s) 739, 995, 1204
Cfr13I GGNCC 1 cut(s) 1452
CseI GACGC 1 cut(s) 1403
Csp6I GTAC 3 cut(s) 871, 910, 1336
CspAI ACCGGT 1 cut(s) 995
CviQI GTAC 3 cut(s) 871, 910, 1336
DdeI CTNAG 2 cut(s) 1223, 1388
DpnI GATC 4 cut(s) 245, 390, 593, 708
DpnII GATC 4 cut(s) 243, 388, 591, 706
DrdI GACNNNNNNGTC 1 cut(s) 475
DseDI GACNNNNNNGTC 1 cut(s) 475
Eam1104I CTCTTC 2 cut(s) 184, 292
EarI CTCTTC 2 cut(s) 184, 292
EciI GGCGGA 2 cut(s) 44, 308
Eco147I AGGCCT 1 cut(s) 563
Eco31I GGTCTC 1 cut(s) 97
Eco47I GGWCC 1 cut(s) 1452
Eco57I CTGAAG 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 1452
EcoRI GAATTC 2 cut(s) 212, 644
EcoRII CCWGG 2 cut(s) 15, 239
Esp3I CGTCTC 1 cut(s) 1386
FaqI GGGAC 2 cut(s) 1008, 1021
FauI CCCGC 1 cut(s) 897
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 5 cut(s) 256, 268, 305, 529, 801
FokI GGATG 1 cut(s) 936
Fsp4HI GCNGC 5 cut(s) 256, 268, 305, 529, 801
FspBI CTAG 2 cut(s) 1157, 1459
GlaI GCGC 2 cut(s) 575, 577
GluI GCNGC 5 cut(s) 256, 268, 305, 529, 801
GsuI CTGGAG 1 cut(s) 38
HaeIII GGCC 2 cut(s) 563, 1208
HapII CCGG 6 cut(s) 231, 614, 740, 996, 1096, 1205
HgaI GACGC 1 cut(s) 1403
HhaI GCGC 2 cut(s) 576, 578
Hin6I GCGC 2 cut(s) 574, 576
HinP1I GCGC 2 cut(s) 574, 576
HincII GTYRAC 1 cut(s) 1060
HindII GTYRAC 1 cut(s) 1060
HindIII AAGCTT 2 cut(s) 727, 1140
HinfI GANTC 6 cut(s) 69, 368, 1065, 1086, 1099, 1344
HpaII CCGG 6 cut(s) 231, 614, 740, 996, 1096, 1205
HphI GGTGA 4 cut(s) 556, 628, 712, 1201
Hpy166II GTNNAC 3 cut(s) 829, 1060, 1166
Hpy188I TCNGA 4 cut(s) 95, 211, 1224, 1391
Hpy8I GTNNAC 3 cut(s) 829, 1060, 1166
Hpy99I CGWCG 1 cut(s) 332
HpyAV CCTTC 6 cut(s) 100, 193, 482, 767, 1050, 1064
HpyCH4III ACNGT 2 cut(s) 125, 379
HpyCH4IV ACGT 2 cut(s) 711, 1338
HpyF10VI GCNNNNNNNGC 8 cut(s) 264, 301, 313, 322, 407, 726, 806, 1428
HpyF3I CTNAG 2 cut(s) 1223, 1388
HpySE526I ACGT 2 cut(s) 711, 1338
HspAI GCGC 2 cut(s) 574, 576
KflI GGGWCCC 1 cut(s) 1452
KroI GCCGGC 1 cut(s) 1204
KroNI GCCGGC 1 cut(s) 1206
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 4 cut(s) 243, 388, 591, 706
LmnI GCTCC 2 cut(s) 232, 321
Lsp1109I GCAGC 5 cut(s) 254, 267, 316, 515, 812
LweI GCATC 8 cut(s) 397, 499, 726, 787, 955, 1096, 1119, 1225
MaeI CTAG 2 cut(s) 1157, 1459
MaeII ACGT 2 cut(s) 711, 1338
MaeIII GTNAC 4 cut(s) 53, 125, 616, 712
MalI GATC 4 cut(s) 245, 390, 593, 708
MboI GATC 4 cut(s) 243, 388, 591, 706
MboII GAAGA 9 cut(s) 46, 171, 279, 416, 559, 782, 1028, 1138, 1244
MfeI CAATTG 1 cut(s) 1426
MflI RGATCY 1 cut(s) 388
MmeI TCCRAC 1 cut(s) 1177
MroNI GCCGGC 1 cut(s) 1204
MseI TTAA 5 cut(s) 132, 165, 606, 744, 756
MslI CAYNNNNRTG 2 cut(s) 969, 1190
MspA1I CMGCKG 1 cut(s) 848
MspI CCGG 6 cut(s) 231, 614, 740, 996, 1096, 1205
MspR9I CCNGG 3 cut(s) 17, 231, 241
MunI CAATTG 1 cut(s) 1426
MvaI CCWGG 2 cut(s) 17, 241
MvnI CGCG 2 cut(s) 576, 1416
MwoI GCNNNNNNNGC 8 cut(s) 264, 301, 313, 322, 407, 726, 806, 1428
NaeI GCCGGC 1 cut(s) 1206
NciI CCSGG 1 cut(s) 231
NdeII GATC 4 cut(s) 243, 388, 591, 706
NgoMIV GCCGGC 1 cut(s) 1204
NlaIV GGNNCC 4 cut(s) 1094, 1150, 1453, 1454
NmuCI GTSAC 1 cut(s) 616
NruI TCGCGA 1 cut(s) 1416
NspI RCATGY 1 cut(s) 724
NspV TTCGAA 2 cut(s) 1068, 1342
PaeI GCATGC 1 cut(s) 724
PagI TCATGA 2 cut(s) 964, 1102
PauI GCGCGC 1 cut(s) 574
PceI AGGCCT 1 cut(s) 563
PcsI WCGNNNNNNNCGW 2 cut(s) 121, 633
PdiI GCCGGC 1 cut(s) 1206
PfeI GAWTC 6 cut(s) 69, 368, 1065, 1086, 1099, 1344
PfoI TCCNGGA 1 cut(s) 15
PinAI ACCGGT 1 cut(s) 995
PkrI GCNGC 5 cut(s) 257, 269, 306, 530, 802
Ple19I CGATCG 1 cut(s) 594
PpuMI RGGWCCY 1 cut(s) 1452
Psp5II RGGWCCY 1 cut(s) 1452
Psp6I CCWGG 2 cut(s) 15, 239
PspGI CCWGG 2 cut(s) 15, 239
PspN4I GGNNCC 4 cut(s) 1094, 1150, 1453, 1454
PspPI GGNCC 1 cut(s) 1452
PspPPI RGGWCCY 1 cut(s) 1452
PstI CTGCAG 1 cut(s) 917
PsuI RGATCY 1 cut(s) 388
PteI GCGCGC 1 cut(s) 574
PvuI CGATCG 1 cut(s) 594
RruI TCGCGA 1 cut(s) 1416
RsaI GTAC 3 cut(s) 872, 911, 1337
RsaNI GTAC 3 cut(s) 871, 910, 1336
RseI CAYNNNNRTG 2 cut(s) 969, 1190
SaqAI TTAA 5 cut(s) 132, 165, 606, 744, 756
SatI GCNGC 5 cut(s) 256, 268, 305, 529, 801
Sau3AI GATC 4 cut(s) 243, 388, 591, 706
Sau96I GGNCC 1 cut(s) 1452
ScrFI CCNGG 3 cut(s) 17, 231, 241
SfaNI GCATC 8 cut(s) 397, 499, 726, 787, 955, 1096, 1119, 1225
SfcI CTRYAG 2 cut(s) 453, 913
SfuI TTCGAA 2 cut(s) 1068, 1342
SinI GGWCC 1 cut(s) 1452
SmiMI CAYNNNNRTG 2 cut(s) 969, 1190
SmlI CTYRAG 1 cut(s) 1439
SmoI CTYRAG 1 cut(s) 1439
SphI GCATGC 1 cut(s) 724
SseBI AGGCCT 1 cut(s) 563
SspMI CTAG 2 cut(s) 1157, 1459
StuI AGGCCT 1 cut(s) 563
StyD4I CCNGG 3 cut(s) 15, 229, 239
TaaI ACNGT 2 cut(s) 125, 379
TaiI ACGT 2 cut(s) 714, 1341
TaqI TCGA 8 cut(s) 67, 101, 371, 498, 594, 1068, 1181, 1342
TatI WGTACW 2 cut(s) 870, 909
TfiI GAWTC 6 cut(s) 69, 368, 1065, 1086, 1099, 1344
Tru1I TTAA 5 cut(s) 132, 165, 606, 744, 756
Tru9I TTAA 5 cut(s) 132, 165, 606, 744, 756
TscAI CASTG 2 cut(s) 448, 1173
TseFI GTSAC 1 cut(s) 616
TseI GCWGC 5 cut(s) 255, 267, 304, 528, 800
Tsp45I GTSAC 1 cut(s) 616
TspDTI ATGAA 4 cut(s) 175, 848, 1245, 1256
TspGWI ACGGA 4 cut(s) 41, 151, 268, 993
TspRI CASTG 2 cut(s) 448, 1173
VpaK11BI GGWCC 1 cut(s) 1452
XapI RAATTY 8 cut(s) 96, 212, 494, 644, 759, 1268, 1356, 1367
XceI RCATGY 1 cut(s) 724
XspI CTAG 2 cut(s) 1157, 1459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.