pycom14g11430

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001127
Physical Location & Seq
Forward (+)
14510297 .. 14512739
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1788 bp
ATGCTGCAGCAAGAAATTAGCCTCGGAATTGATGGTATACAGTTACAAACATGGTTTCTCTGGGTTTGCAGCCAAGCTGACAGATTGCCTGGTGTTGTACGAATCATACCAAATAGCCTTCATAAGTTGGAAACAACCAGGAGTTGGGATTTTCTTGGCCTCTCTCCTCACTCCCCTTCCAATATTCTCCCCAAGAGCAACATGGGGGATGGAGTTATCATAGGTGTTCTTGACACAGGTATATGGCCGGAGTCTGAGTCATTCAATGAAAAAGGCCTCGGGCCTGTTCCATCTCATTGGAATGGTGTTTGTGAATCTGGGGACAATTTCAATGCTACAATACATTGCAATAGAAAAATCATCGGAGCCCGTTGGTTTATCAATGGACTTCTTGCCGAGTATGCAAAGCCATTGGACAAAGAATTCCATTCTCCTAGAGATGCACATGGACATGGTACTCATACCTCAAGCACTGCAGCTGGTTCTTTTGTGGCTAATATCAGCTACAAGGGCCTTGGTCTTGGTACAATTAGAGGCGGTGCTCCAAATGCTCGGTTTGCCATTTACAAGGTTTGTTGGAATGTGCTTGGCGGCCAATGCTCAACAGCTGATATTTTGAAAGCTTTTGACGAAGCAATACACGATGGGGTTGATGTGTTGTCATTGTCAATTGTGTATTCTGTTCCCCTCTTCTCAGATGTTGATGAGCGTGATGGCATTGCAACTGGTTCCTTTCATGCTGTGGCTAAGGGGATTACTGTTGTGTGCGCAGCTGGAAATAGTGGACCTTCAGCACAAACGGTTGTGAACACGGCACCGTGGATCATAACTGTTGCAGCAAGCACCATGGATCGAGAATTTCCTACTTCTATAACTTTAGGAAATAACAAAACTTTCCTGGTATGTGTTTTTGTGATAGGGGGTCAAGCTATGTTTACAGGGACAGAGATAGGATTTACAAACTTGGTATACCCACAGTCTGAAGGGCCTACCGCTGGAGGGTATGTTGCATTCTTAATTACTTTGTGCCAGATCCTTACAATTTGCCTAATTTACTATATTATATTTATCATTCGTCTTCACGAGTTGCTTGTTTTCAGTGTGTGTGAATCCCTCTCACTCAACAAAACTATTGTAGTTGGAAAGGTGGTTCTCTGCTTCACTACAATGGGTCGTCGAGCGATAACAAATGCTTCAGCAGTTGTGAAAGAAGCAGGAGGTGTTGGCCTAATCATCGCAAAGAATCCAAGTGGTGCTTTATCTCCATGTATTGAAGACTTTCCATGCATTGAAGTTGACTATGAGATTGGCACTCGAATTCTATTTTATATCCGGTCAACCAGGTACGGAACTTTAAATTTTATTGTAAAATGTGTGATTAGTACTGCTAGGTTTGTTTGTAATCCTCAAACTTTGTTTGGAGTGCACAGATATCCTCTTGTAAAGCTGAGCCTTCCCAAAACGACTGTTGGAAAACCACTATCTGCCAAGGTGGCTTATTTTTCCTCTAGGGGGCCAAACTCCATTGCACCAGCAAGTCTCAAGCCAGATATAGCCGCGCCTGGTGTGAACATACTAGCAGCAACTTCTCCACTTTATTCATTCGCGGAGGGTGGATATGCCATGATGTCTGGAACATCAATGTCAACTCCTCATGTCACAGGCATTGTGGCTCTTATCAAGAGAATGCATCCTAATTGGTCTCCAGCGGCCATCAAATCAGCACTAGTAACAACTGGTAATCACATATTATATGATATGTTCCCAGTTTCAATAAGATACATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

596

Amino Acids

63.89

Weight (kDa)

8.28

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S8 PF00082 69 - 580 2e-45 Subtilase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 769
AccB1I GGYRCC 1 cut(s) 814
AccB7I CCANNNNNTGG 1 cut(s) 144
AccI GTMKAC 2 cut(s) 37, 969
AccII CGCG 2 cut(s) 1559, 1607
AciI CCGC 6 cut(s) 537, 591, 993, 1557, 1607, 1709
AclWI GGATC 3 cut(s) 830, 858, 1027
AcoI YGGCCR 3 cut(s) 245, 592, 1710
AcsI RAATTY 4 cut(s) 422, 857, 1317, 1357
AcuI CTGAAG 3 cut(s) 774, 1002, 1179
AfaI GTAC 5 cut(s) 99, 457, 526, 1346, 1384
AfiI CCNNNNNNNGG 4 cut(s) 144, 995, 999, 1512
AflIII ACRYGT 1 cut(s) 1782
AgsI TTSAA 6 cut(s) 265, 331, 619, 1274, 1292, 1773
AhlI ACTAGT 1 cut(s) 1726
AjnI CCWGG 5 cut(s) 88, 137, 897, 1340, 1561
AloI GAACNNNNNNTCC 2 cut(s) 1134, 1166
AluBI AGCT 8 cut(s) 77, 479, 504, 608, 623, 773, 929, 1447
AluI AGCT 8 cut(s) 77, 479, 504, 608, 623, 773, 929, 1447
Alw21I GWGCWC 2 cut(s) 544, 1428
Alw26I GTCTC 2 cut(s) 1544, 1707
Alw44I GTGCAC 1 cut(s) 1424
AlwI GGATC 3 cut(s) 830, 858, 1027
Ama87I CYCGRG 1 cut(s) 278
ApaLI GTGCAC 1 cut(s) 1424
ApeKI GCWGC 7 cut(s) 4, 7, 69, 476, 770, 836, 1580
ApoI RAATTY 4 cut(s) 422, 857, 1317, 1357
Asp700I GAANNNNTTC 1 cut(s) 1278
AspLEI GCGC 2 cut(s) 770, 1561
AspS9I GGNCC 5 cut(s) 281, 511, 785, 986, 1514
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 1 cut(s) 785
BaeGI GKGCMC 1 cut(s) 1428
BanI GGYRCC 1 cut(s) 814
BanII GRGCYC 1 cut(s) 370
BauI CACGAG 1 cut(s) 1082
BbsI GAAGAC 2 cut(s) 1070, 1281
Bbv12I GWGCWC 2 cut(s) 544, 1428
BbvI GCAGC 6 cut(s) 19, 81, 488, 782, 848, 1592
BccI CCATC 6 cut(s) 26, 203, 298, 638, 707, 1721
BceAI ACGGC 1 cut(s) 828
BciT130I CCWGG 5 cut(s) 90, 139, 899, 1342, 1563
BcoDI GTCTC 2 cut(s) 1544, 1707
BcuI ACTAGT 1 cut(s) 1726
BfaI CTAG 5 cut(s) 435, 1389, 1509, 1577, 1727
BfmI CTRYAG 2 cut(s) 5, 474
BglI GCCNNNNNGGC 1 cut(s) 1493
BlpI GCTNAGC 1 cut(s) 1448
BmcAI AGTACT 1 cut(s) 1384
Bme1390I CCNGG 5 cut(s) 90, 139, 899, 1342, 1563
Bme18I GGWCC 1 cut(s) 785
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 5 cut(s) 281, 511, 785, 986, 1514
BmiI GGNNCC 4 cut(s) 367, 730, 816, 1515
BmrFI CCNGG 5 cut(s) 90, 139, 899, 1342, 1563
BmrI ACTGGG 1 cut(s) 1760
BmsI GCATC 2 cut(s) 430, 1699
BmuI ACTGGG 1 cut(s) 1760
BpiI GAAGAC 2 cut(s) 1070, 1281
BpmI CTGGAG 2 cut(s) 1017, 1689
Bpu10I CCTNAGC 1 cut(s) 747
Bpu1102I GCTNAGC 1 cut(s) 1448
BpuEI CTTGAG 2 cut(s) 451, 1526
BsaI GGTCTC 1 cut(s) 1707
BsaJI CCNNGG 6 cut(s) 22, 277, 514, 818, 846, 1488
BsaWI WCCGGW 1 cut(s) 1332
BsaXI ACNNNNNCTCC 4 cut(s) 242, 272, 990, 1020
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 995, 999, 1512
Bse1I ACTGG 3 cut(s) 730, 1741, 1766
Bse3DI GCAATG 3 cut(s) 343, 717, 1524
BseBI CCWGG 5 cut(s) 90, 139, 899, 1342, 1563
BseDI CCNNGG 6 cut(s) 22, 277, 514, 818, 846, 1488
BseGI GGATG 2 cut(s) 214, 1690
BseLI CCNNNNNNNGG 4 cut(s) 144, 995, 999, 1512
BseMI GCAATG 3 cut(s) 343, 717, 1524
BseMII CTCAG 3 cut(s) 246, 708, 1439
BseNI ACTGG 3 cut(s) 730, 1741, 1766
BseRI GAGGAG 2 cut(s) 156, 1641
BseSI GKGCMC 1 cut(s) 1428
BseXI GCAGC 6 cut(s) 19, 81, 488, 782, 848, 1592
Bsh1236I CGCG 2 cut(s) 1559, 1607
BshNI GGYRCC 1 cut(s) 814
BsiHKAI GWGCWC 2 cut(s) 544, 1428
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 2 cut(s) 248, 1333
BslFI GGGAC 2 cut(s) 335, 955
BslI CCNNNNNNNGG 4 cut(s) 144, 995, 999, 1512
BsmAI GTCTC 2 cut(s) 1544, 1707
BsmFI GGGAC 2 cut(s) 335, 955
BsmI GAATGC 2 cut(s) 1010, 1692
Bso31I GGTCTC 1 cut(s) 1707
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 3 cut(s) 370, 544, 1428
Bsp143I GATC 3 cut(s) 822, 850, 1032
Bsp1720I GCTNAGC 1 cut(s) 1448
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 6 cut(s) 537, 591, 993, 1557, 1607, 1709
BspCNI CTCAG 3 cut(s) 247, 707, 1440
BspFNI CGCG 2 cut(s) 1559, 1607
BspLI GGNNCC 4 cut(s) 367, 730, 816, 1515
BspMAI CTGCAG 2 cut(s) 9, 478
BspPI GGATC 3 cut(s) 830, 858, 1027
BspT107I GGYRCC 1 cut(s) 814
BspTNI GGTCTC 1 cut(s) 1707
BsrDI GCAATG 3 cut(s) 343, 717, 1524
BsrI ACTGG 3 cut(s) 730, 1741, 1766
BssECI CCNNGG 6 cut(s) 22, 277, 514, 818, 846, 1488
BssMI GATC 3 cut(s) 822, 850, 1032
BssNAI GTATAC 2 cut(s) 38, 970
BssSI CACGAG 1 cut(s) 1082
BssT1I CCWWGG 3 cut(s) 514, 846, 1488
Bst1107I GTATAC 2 cut(s) 38, 970
Bst2BI CACGAG 1 cut(s) 1082
Bst2UI CCWGG 5 cut(s) 90, 139, 899, 1342, 1563
Bst4CI ACNGT 7 cut(s) 42, 760, 802, 819, 832, 978, 1468
Bst6I CTCTTC 1 cut(s) 695
BstC8I GCNNGC 1 cut(s) 841
BstDEI CTNAG 4 cut(s) 255, 694, 747, 1448
BstDSI CCRYGG 2 cut(s) 818, 846
BstF5I GGATG 2 cut(s) 214, 1690
BstFNI CGCG 2 cut(s) 1559, 1607
BstHHI GCGC 2 cut(s) 770, 1561
BstKTI GATC 3 cut(s) 825, 853, 1035
BstMAI GTCTC 2 cut(s) 1544, 1707
BstMBI GATC 3 cut(s) 822, 850, 1032
BstMWI GCNNNNNNNGC 6 cut(s) 401, 510, 548, 557, 597, 1493
BstNI CCWGG 5 cut(s) 90, 139, 899, 1342, 1563
BstNSI RCATGY 1 cut(s) 1786
BstSCI CCNGG 5 cut(s) 88, 137, 897, 1340, 1561
BstSFI CTRYAG 2 cut(s) 5, 474
BstSLI GKGCMC 1 cut(s) 1428
BstUI CGCG 2 cut(s) 1559, 1607
BstV1I GCAGC 6 cut(s) 19, 81, 488, 782, 848, 1592
BstV2I GAAGAC 2 cut(s) 1070, 1281
BstX2I RGATCY 1 cut(s) 1032
BstXI CCANNNNNNTGG 1 cut(s) 297
BstYI RGATCY 1 cut(s) 1032
BstZ17I GTATAC 2 cut(s) 38, 970
BtgI CCRYGG 2 cut(s) 818, 846
BtgZI GCGATG 1 cut(s) 1219
BtsCI GGATG 2 cut(s) 214, 1690
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 2 cut(s) 471, 1105
Cac8I GCNNGC 1 cut(s) 841
CfoI GCGC 2 cut(s) 770, 1561
Cfr13I GGNCC 5 cut(s) 281, 511, 785, 986, 1514
CsiI ACCWGGT 1 cut(s) 1340
Csp6I GTAC 5 cut(s) 98, 456, 525, 1345, 1383
CviQI GTAC 5 cut(s) 98, 456, 525, 1345, 1383
DdeI CTNAG 4 cut(s) 255, 694, 747, 1448
DpnI GATC 3 cut(s) 824, 852, 1034
DpnII GATC 3 cut(s) 822, 850, 1032
DraI TTTAAA 1 cut(s) 1356
EaeI YGGCCR 3 cut(s) 245, 592, 1710
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
Eco130I CCWWGG 3 cut(s) 514, 846, 1488
Eco147I AGGCCT 1 cut(s) 276
Eco24I GRGCYC 1 cut(s) 370
Eco31I GGTCTC 1 cut(s) 1707
Eco32I GATATC 1 cut(s) 1433
Eco47I GGWCC 1 cut(s) 785
Eco57I CTGAAG 3 cut(s) 774, 1002, 1179
Eco88I CYCGRG 1 cut(s) 278
EcoO109I RGGNCCY 2 cut(s) 511, 986
EcoRI GAATTC 2 cut(s) 422, 1317
EcoRII CCWGG 5 cut(s) 88, 137, 897, 1340, 1561
EcoRV GATATC 1 cut(s) 1433
EcoT14I CCWWGG 3 cut(s) 514, 846, 1488
EcoT22I ATGCAT 2 cut(s) 1289, 1692
EcoT38I GRGCYC 1 cut(s) 370
ErhI CCWWGG 3 cut(s) 514, 846, 1488
FalI AAGNNNNNCTT 2 cut(s) 1240, 1272
FaqI GGGAC 2 cut(s) 335, 955
FblI GTMKAC 2 cut(s) 37, 969
FokI GGATG 2 cut(s) 221, 1677
FriOI GRGCYC 1 cut(s) 370
FspBI CTAG 5 cut(s) 435, 1389, 1509, 1577, 1727
FspI TGCGCA 1 cut(s) 769
GlaI GCGC 2 cut(s) 769, 1560
GsuI CTGGAG 2 cut(s) 1017, 1689
HapII CCGG 2 cut(s) 248, 1333
HhaI GCGC 2 cut(s) 770, 1561
Hin6I GCGC 2 cut(s) 768, 1559
HinP1I GCGC 2 cut(s) 768, 1559
HincII GTYRAC 3 cut(s) 1297, 1338, 1647
HindII GTYRAC 3 cut(s) 1297, 1338, 1647
HindIII AAGCTT 1 cut(s) 621
HinfI GANTC 6 cut(s) 102, 251, 257, 314, 1109, 1243
HpaII CCGG 2 cut(s) 248, 1333
Hpy188I TCNGA 5 cut(s) 26, 256, 365, 697, 982
Hpy188III TCNNGA 5 cut(s) 230, 854, 1082, 1632, 1681
Hpy99I CGWCG 1 cut(s) 1179
HpyAV CCTTC 5 cut(s) 128, 186, 798, 977, 1463
HpyCH4III ACNGT 7 cut(s) 42, 760, 802, 819, 832, 978, 1468
HpyF10VI GCNNNNNNNGC 6 cut(s) 401, 510, 548, 557, 597, 1493
HpyF3I CTNAG 4 cut(s) 255, 694, 747, 1448
HspAI GCGC 2 cut(s) 768, 1559
Kzo9I GATC 3 cut(s) 822, 850, 1032
LmnI GCTCC 2 cut(s) 365, 547
Lsp1109I GCAGC 6 cut(s) 19, 81, 488, 782, 848, 1592
LweI GCATC 2 cut(s) 430, 1699
MabI ACCWGGT 1 cut(s) 1340
MaeI CTAG 5 cut(s) 435, 1389, 1509, 1577, 1727
MaeIII GTNAC 3 cut(s) 42, 1657, 1729
MalI GATC 3 cut(s) 824, 852, 1034
MboI GATC 3 cut(s) 822, 850, 1032
MboII GAAGA 3 cut(s) 682, 1070, 1286
MfeI CAATTG 1 cut(s) 669
MflI RGATCY 1 cut(s) 1032
MhlI GDGCHC 3 cut(s) 370, 544, 1428
MlyI GAGTC 2 cut(s) 260, 266
MmeI TCCRAC 4 cut(s) 108, 557, 1120, 1450
Mph1103I ATGCAT 2 cut(s) 1289, 1692
MroXI GAANNNNTTC 1 cut(s) 1278
MseI TTAA 2 cut(s) 1016, 1355
MslI CAYNNNNRTG 3 cut(s) 300, 450, 1166
MspA1I CMGCKG 5 cut(s) 479, 608, 773, 995, 1709
MspI CCGG 2 cut(s) 248, 1333
MspR9I CCNGG 5 cut(s) 90, 139, 899, 1342, 1563
MunI CAATTG 1 cut(s) 669
Mva1269I GAATGC 2 cut(s) 1010, 1692
MvaI CCWGG 5 cut(s) 90, 139, 899, 1342, 1563
MvnI CGCG 2 cut(s) 1559, 1607
MwoI GCNNNNNNNGC 6 cut(s) 401, 510, 548, 557, 597, 1493
NcoI CCATGG 1 cut(s) 846
NdeII GATC 3 cut(s) 822, 850, 1032
NlaIV GGNNCC 4 cut(s) 367, 730, 816, 1515
NmeAIII GCCGAG 1 cut(s) 421
NmuCI GTSAC 1 cut(s) 1657
NsbI TGCGCA 1 cut(s) 769
NsiI ATGCAT 2 cut(s) 1289, 1692
NspI RCATGY 1 cut(s) 1786
PceI AGGCCT 1 cut(s) 276
PciI ACATGT 1 cut(s) 1782
PctI GAATGC 2 cut(s) 1010, 1692
PdmI GAANNNNTTC 1 cut(s) 1278
PfeI GAWTC 4 cut(s) 102, 314, 1109, 1243
PflMI CCANNNNNTGG 1 cut(s) 144
PleI GAGTC 2 cut(s) 259, 265
PpsI GAGTC 2 cut(s) 259, 265
PscI ACATGT 1 cut(s) 1782
Psp6I CCWGG 5 cut(s) 88, 137, 897, 1340, 1561
PspGI CCWGG 5 cut(s) 88, 137, 897, 1340, 1561
PspN4I GGNNCC 4 cut(s) 367, 730, 816, 1515
PspPI GGNCC 5 cut(s) 281, 511, 785, 986, 1514
PstI CTGCAG 2 cut(s) 9, 478
PsuI RGATCY 1 cut(s) 1032
PvuII CAGCTG 3 cut(s) 479, 608, 773
RsaI GTAC 5 cut(s) 99, 457, 526, 1346, 1384
RsaNI GTAC 5 cut(s) 98, 456, 525, 1345, 1383
RseI CAYNNNNRTG 3 cut(s) 300, 450, 1166
SaqAI TTAA 2 cut(s) 1016, 1355
Sau3AI GATC 3 cut(s) 822, 850, 1032
Sau96I GGNCC 5 cut(s) 281, 511, 785, 986, 1514
ScaI AGTACT 1 cut(s) 1384
SchI GAGTC 2 cut(s) 260, 266
ScrFI CCNGG 5 cut(s) 90, 139, 899, 1342, 1563
SduI GDGCHC 3 cut(s) 370, 544, 1428
SexAI ACCWGGT 1 cut(s) 1340
SfaNI GCATC 2 cut(s) 430, 1699
SfcI CTRYAG 2 cut(s) 5, 474
SinI GGWCC 1 cut(s) 785
SmiMI CAYNNNNRTG 3 cut(s) 300, 450, 1166
SmlI CTYRAG 2 cut(s) 466, 1541
SmoI CTYRAG 2 cut(s) 466, 1541
SpeI ACTAGT 1 cut(s) 1726
SseBI AGGCCT 1 cut(s) 276
SsiI CCGC 6 cut(s) 537, 591, 993, 1557, 1607, 1709
SspI AATATT 1 cut(s) 184
SspMI CTAG 5 cut(s) 435, 1389, 1509, 1577, 1727
StuI AGGCCT 1 cut(s) 276
StyD4I CCNGG 5 cut(s) 88, 137, 897, 1340, 1561
StyI CCWWGG 3 cut(s) 514, 846, 1488
TaaI ACNGT 7 cut(s) 42, 760, 802, 819, 832, 978, 1468
TaqI TCGA 3 cut(s) 853, 1177, 1315
TatI WGTACW 1 cut(s) 1382
TauI GCSGC 3 cut(s) 594, 1559, 1712
TfiI GAWTC 4 cut(s) 102, 314, 1109, 1243
Tru1I TTAA 2 cut(s) 1016, 1355
Tru9I TTAA 2 cut(s) 1016, 1355
TscAI CASTG 2 cut(s) 478, 1105
TseFI GTSAC 1 cut(s) 1657
TseI GCWGC 7 cut(s) 4, 7, 69, 476, 770, 836, 1580
Tsp45I GTSAC 1 cut(s) 1657
TspDTI ATGAA 4 cut(s) 110, 282, 725, 1590
TspGWI ACGGA 1 cut(s) 1362
TspRI CASTG 2 cut(s) 478, 1105
Van91I CCANNNNNTGG 1 cut(s) 144
VneI GTGCAC 1 cut(s) 1424
VpaK11BI GGWCC 1 cut(s) 785
XapI RAATTY 4 cut(s) 422, 857, 1317, 1357
XceI RCATGY 1 cut(s) 1786
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmiI GTMKAC 2 cut(s) 37, 969
XmnI GAANNNNTTC 1 cut(s) 1278
XspI CTAG 5 cut(s) 435, 1389, 1509, 1577, 1727
ZrmI AGTACT 1 cut(s) 1384
Zsp2I ATGCAT 2 cut(s) 1289, 1692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.