subtilisin-like protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 4 cut(s) | 210, 1152, 1421, 1845 |
| AccII | CGCG | 2 cut(s) | 1556, 1604 |
| AciI | CCGC | 3 cut(s) | 735, 1554, 1604 |
| AclWI | GGATC | 4 cut(s) | 1028, 1056, 1760, 1769 |
| AcoI | YGGCCR | 2 cut(s) | 443, 756 |
| AcsI | RAATTY | 4 cut(s) | 620, 1055, 1401, 1783 |
| AcuI | CTGAAG | 2 cut(s) | 972, 1263 |
| AfaI | GTAC | 7 cut(s) | 93, 297, 655, 724, 1396, 1895, 2089 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 2084, 2212 |
| AflIII | ACRYGT | 1 cut(s) | 1937 |
| AgeI | ACCGGT | 1 cut(s) | 923 |
| AgsI | TTSAA | 8 cut(s) | 18, 463, 529, 817, 1376, 2075, 2125, 2138 |
| AhlI | ACTAGT | 1 cut(s) | 1840 |
| AjnI | CCWGG | 4 cut(s) | 286, 1095, 1558, 1834 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2194, 2226 |
| AleI | CACNNNNGTG | 1 cut(s) | 173 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1218, 1250 |
| Alw21I | GWGCWC | 2 cut(s) | 742, 968 |
| Alw26I | GTCTC | 2 cut(s) | 264, 1704 |
| AlwI | GGATC | 4 cut(s) | 1028, 1056, 1760, 1769 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1835 |
| Ama87I | CYCGRG | 1 cut(s) | 476 |
| AoxI | GGCC | 8 cut(s) | 355, 443, 472, 479, 709, 756, 1309, 1730 |
| ApoI | RAATTY | 4 cut(s) | 620, 1055, 1401, 1783 |
| AsiGI | ACCGGT | 1 cut(s) | 923 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1362 |
| AspLEI | GCGC | 2 cut(s) | 1558, 1826 |
| AspS9I | GGNCC | 5 cut(s) | 479, 709, 983, 1511, 1731 |
| AsuC2I | CCSGG | 1 cut(s) | 517 |
| AsuHPI | GGTGA | 2 cut(s) | 354, 1822 |
| AsuII | TTCGAA | 1 cut(s) | 1004 |
| AvaI | CYCGRG | 1 cut(s) | 476 |
| AvaII | GGWCC | 2 cut(s) | 983, 1511 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2079, 2112 |
| BalI | TGGCCA | 1 cut(s) | 758 |
| BanII | GRGCYC | 3 cut(s) | 568, 1451, 2047 |
| BbsI | GAAGAC | 3 cut(s) | 1013, 1365, 2113 |
| Bbv12I | GWGCWC | 2 cut(s) | 742, 968 |
| BccI | CCATC | 9 cut(s) | 199, 401, 496, 836, 905, 1246, 1718, 1742, 1916 |
| BceAI | ACGGC | 2 cut(s) | 1745, 2216 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1744, 1778, 1838, 1872 |
| BciT130I | CCWGG | 4 cut(s) | 288, 1097, 1560, 1836 |
| BciVI | GTATCC | 1 cut(s) | 885 |
| BcnI | CCSGG | 1 cut(s) | 517 |
| BcoDI | GTCTC | 2 cut(s) | 264, 1704 |
| BcuI | ACTAGT | 1 cut(s) | 1840 |
| BfaI | CTAG | 7 cut(s) | 336, 633, 1506, 1574, 1724, 1841, 1995 |
| BfmI | CTRYAG | 2 cut(s) | 672, 1215 |
| BfuI | GTATCC | 1 cut(s) | 885 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1490 |
| BlpI | GCTNAGC | 2 cut(s) | 967, 1445 |
| Bme1390I | CCNGG | 5 cut(s) | 288, 517, 1097, 1560, 1836 |
| Bme18I | GGWCC | 2 cut(s) | 983, 1511 |
| BmeT110I | CYCGRG | 1 cut(s) | 476 |
| BmgT120I | GGNCC | 5 cut(s) | 479, 709, 983, 1511, 1731 |
| BmiI | GGNNCC | 4 cut(s) | 565, 928, 1512, 2044 |
| BmrFI | CCNGG | 5 cut(s) | 288, 517, 1097, 1560, 1836 |
| BmsI | GCATC | 3 cut(s) | 628, 1327, 1696 |
| BpiI | GAAGAC | 3 cut(s) | 1013, 1365, 2113 |
| BpmI | CTGGAG | 1 cut(s) | 1686 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 967, 1445 |
| Bpu14I | TTCGAA | 1 cut(s) | 1004 |
| BpuEI | CTTGAG | 2 cut(s) | 649, 1523 |
| BpuMI | CCSGG | 1 cut(s) | 517 |
| Bsa29I | ATCGAT | 1 cut(s) | 1764 |
| BsaBI | GATNNNNATC | 1 cut(s) | 150 |
| BsaI | GGTCTC | 1 cut(s) | 1704 |
| BsaJI | CCNNGG | 8 cut(s) | 195, 475, 516, 712, 1016, 1044, 1485, 1834 |
| BsaWI | WCCGGW | 2 cut(s) | 923, 1416 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 2084, 2212 |
| Bse118I | RCCGGY | 1 cut(s) | 923 |
| Bse1I | ACTGG | 2 cut(s) | 55, 2191 |
| Bse3DI | GCAATG | 2 cut(s) | 541, 915 |
| Bse8I | GATNNNNATC | 1 cut(s) | 150 |
| BseBI | CCWGG | 4 cut(s) | 288, 1097, 1560, 1836 |
| BseCI | ATCGAT | 1 cut(s) | 1764 |
| BseDI | CCNNGG | 8 cut(s) | 195, 475, 516, 712, 1016, 1044, 1485, 1834 |
| BseGI | GGATG | 2 cut(s) | 412, 1687 |
| BseJI | GATNNNNATC | 1 cut(s) | 150 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 2084, 2212 |
| BseMI | GCAATG | 2 cut(s) | 541, 915 |
| BseMII | CTCAG | 7 cut(s) | 46, 906, 981, 1436, 1991, 2040, 2250 |
| BseNI | ACTGG | 2 cut(s) | 55, 2191 |
| BsgI | GTGCAG | 1 cut(s) | 1878 |
| Bsh1236I | CGCG | 2 cut(s) | 1556, 1604 |
| BshFI | GGCC | 8 cut(s) | 357, 445, 474, 481, 711, 758, 1311, 1732 |
| BshTI | ACCGGT | 1 cut(s) | 923 |
| BshVI | ATCGAT | 1 cut(s) | 1764 |
| BsiHKAI | GWGCWC | 2 cut(s) | 742, 968 |
| BsiHKCI | CYCGRG | 1 cut(s) | 476 |
| BsiSI | CCGG | 4 cut(s) | 446, 516, 924, 1417 |
| BslFI | GGGAC | 4 cut(s) | 533, 1138, 1524, 1926 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 2084, 2212 |
| BsmAI | GTCTC | 2 cut(s) | 264, 1704 |
| BsmFI | GGGAC | 4 cut(s) | 533, 1138, 1524, 1926 |
| BsmI | GAATGC | 1 cut(s) | 1689 |
| BsnI | GGCC | 8 cut(s) | 357, 445, 474, 481, 711, 758, 1311, 1732 |
| Bso31I | GGTCTC | 1 cut(s) | 1704 |
| BsoBI | CYCGRG | 1 cut(s) | 476 |
| Bsp119I | TTCGAA | 1 cut(s) | 1004 |
| Bsp1286I | GDGCHC | 5 cut(s) | 568, 742, 968, 1451, 2047 |
| Bsp1407I | TGTACA | 1 cut(s) | 91 |
| Bsp143I | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 967, 1445 |
| Bsp19I | CCATGG | 1 cut(s) | 1044 |
| BspACI | CCGC | 3 cut(s) | 735, 1554, 1604 |
| BspANI | GGCC | 8 cut(s) | 357, 445, 474, 481, 711, 758, 1311, 1732 |
| BspCNI | CTCAG | 7 cut(s) | 45, 905, 980, 1437, 1992, 2041, 2251 |
| BspDI | ATCGAT | 1 cut(s) | 1764 |
| BspFNI | CGCG | 2 cut(s) | 1556, 1604 |
| BspHI | TCATGA | 2 cut(s) | 154, 2275 |
| BspLI | GGNNCC | 4 cut(s) | 565, 928, 1512, 2044 |
| BspMAI | CTGCAG | 1 cut(s) | 676 |
| BspPI | GGATC | 4 cut(s) | 1028, 1056, 1760, 1769 |
| BspT104I | TTCGAA | 1 cut(s) | 1004 |
| BspTNI | GGTCTC | 1 cut(s) | 1704 |
| BsrDI | GCAATG | 2 cut(s) | 541, 915 |
| BsrFI | RCCGGY | 1 cut(s) | 923 |
| BsrGI | TGTACA | 1 cut(s) | 91 |
| BsrI | ACTGG | 2 cut(s) | 55, 2191 |
| BssAI | RCCGGY | 1 cut(s) | 923 |
| BssECI | CCNNGG | 8 cut(s) | 195, 475, 516, 712, 1016, 1044, 1485, 1834 |
| BssMI | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| BssNAI | GTATAC | 3 cut(s) | 211, 1153, 1846 |
| BssT1I | CCWWGG | 3 cut(s) | 712, 1044, 1485 |
| Bst1107I | GTATAC | 3 cut(s) | 211, 1153, 1846 |
| Bst2UI | CCWGG | 4 cut(s) | 288, 1097, 1560, 1836 |
| Bst6I | CTCTTC | 1 cut(s) | 893 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1304 |
| BstAUI | TGTACA | 1 cut(s) | 91 |
| BstBI | TTCGAA | 1 cut(s) | 1004 |
| BstC8I | GCNNGC | 5 cut(s) | 165, 1039, 1309, 1472, 2229 |
| BstDEI | CTNAG | 7 cut(s) | 32, 892, 967, 1445, 2000, 2049, 2259 |
| BstDSI | CCRYGG | 2 cut(s) | 1016, 1044 |
| BstF5I | GGATG | 2 cut(s) | 412, 1687 |
| BstFNI | CGCG | 2 cut(s) | 1556, 1604 |
| BstHHI | GCGC | 2 cut(s) | 1558, 1826 |
| BstKTI | GATC | 4 cut(s) | 1023, 1051, 1764, 1768 |
| BstMAI | GTCTC | 2 cut(s) | 264, 1704 |
| BstMBI | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 599, 708, 746, 755, 1304, 1490 |
| BstNI | CCWGG | 4 cut(s) | 288, 1097, 1560, 1836 |
| BstNSI | RCATGY | 2 cut(s) | 1941, 2231 |
| BstSCI | CCNGG | 5 cut(s) | 286, 515, 1095, 1558, 1834 |
| BstSFI | CTRYAG | 2 cut(s) | 672, 1215 |
| BstUI | CGCG | 2 cut(s) | 1556, 1604 |
| BstV2I | GAAGAC | 3 cut(s) | 1013, 1365, 2113 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 175, 495 |
| BstZ17I | GTATAC | 3 cut(s) | 211, 1153, 1846 |
| Bsu15I | ATCGAT | 1 cut(s) | 1764 |
| BsuI | GTATCC | 1 cut(s) | 885 |
| BsuRI | GGCC | 8 cut(s) | 357, 445, 474, 481, 711, 758, 1311, 1732 |
| BsuTUI | ATCGAT | 1 cut(s) | 1764 |
| BtgI | CCRYGG | 2 cut(s) | 1016, 1044 |
| BtgZI | GCGATG | 1 cut(s) | 1303 |
| BtsCI | GGATG | 2 cut(s) | 412, 1687 |
| BtsI | GCAGTG | 1 cut(s) | 669 |
| BtsIMutI | CAGTG | 4 cut(s) | 168, 669, 867, 2184 |
| Cac8I | GCNNGC | 5 cut(s) | 165, 1039, 1309, 1472, 2229 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1835 |
| CciI | TCATGA | 2 cut(s) | 154, 2275 |
| CfoI | GCGC | 2 cut(s) | 1558, 1826 |
| Cfr10I | RCCGGY | 1 cut(s) | 923 |
| Cfr13I | GGNCC | 5 cut(s) | 479, 709, 983, 1511, 1731 |
| ClaI | ATCGAT | 1 cut(s) | 1764 |
| Csp6I | GTAC | 7 cut(s) | 92, 296, 654, 723, 1395, 1894, 2088 |
| CspAI | ACCGGT | 1 cut(s) | 923 |
| CviQI | GTAC | 7 cut(s) | 92, 296, 654, 723, 1395, 1894, 2088 |
| DdeI | CTNAG | 7 cut(s) | 32, 892, 967, 1445, 2000, 2049, 2259 |
| DpnI | GATC | 4 cut(s) | 1022, 1050, 1763, 1767 |
| DpnII | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| EaeI | YGGCCR | 2 cut(s) | 443, 756 |
| Eam1104I | CTCTTC | 1 cut(s) | 893 |
| EarI | CTCTTC | 1 cut(s) | 893 |
| Eco130I | CCWWGG | 3 cut(s) | 712, 1044, 1485 |
| Eco147I | AGGCCT | 1 cut(s) | 474 |
| Eco24I | GRGCYC | 3 cut(s) | 568, 1451, 2047 |
| Eco31I | GGTCTC | 1 cut(s) | 1704 |
| Eco32I | GATATC | 2 cut(s) | 697, 1430 |
| Eco47I | GGWCC | 2 cut(s) | 983, 1511 |
| Eco57I | CTGAAG | 2 cut(s) | 972, 1263 |
| Eco88I | CYCGRG | 1 cut(s) | 476 |
| EcoO109I | RGGNCCY | 1 cut(s) | 709 |
| EcoRI | GAATTC | 2 cut(s) | 620, 1401 |
| EcoRII | CCWGG | 4 cut(s) | 286, 1095, 1558, 1834 |
| EcoRV | GATATC | 2 cut(s) | 697, 1430 |
| EcoT14I | CCWWGG | 3 cut(s) | 712, 1044, 1485 |
| EcoT22I | ATGCAT | 2 cut(s) | 1373, 1689 |
| EcoT38I | GRGCYC | 3 cut(s) | 568, 1451, 2047 |
| ErhI | CCWWGG | 3 cut(s) | 712, 1044, 1485 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2194, 2226 |
| FaqI | GGGAC | 4 cut(s) | 533, 1138, 1524, 1926 |
| FblI | GTMKAC | 4 cut(s) | 210, 1152, 1421, 1845 |
| FokI | GGATG | 2 cut(s) | 419, 1674 |
| FriOI | GRGCYC | 3 cut(s) | 568, 1451, 2047 |
| FspBI | CTAG | 7 cut(s) | 336, 633, 1506, 1574, 1724, 1841, 1995 |
| GlaI | GCGC | 2 cut(s) | 1557, 1825 |
| GsuI | CTGGAG | 1 cut(s) | 1686 |
| HaeIII | GGCC | 8 cut(s) | 357, 445, 474, 481, 711, 758, 1311, 1732 |
| HapII | CCGG | 4 cut(s) | 446, 516, 924, 1417 |
| HhaI | GCGC | 2 cut(s) | 1558, 1826 |
| Hin6I | GCGC | 2 cut(s) | 1556, 1824 |
| HinP1I | GCGC | 2 cut(s) | 1556, 1824 |
| HincII | GTYRAC | 3 cut(s) | 1381, 1972, 2218 |
| HindII | GTYRAC | 3 cut(s) | 1381, 1972, 2218 |
| HindIII | AAGCTT | 2 cut(s) | 819, 1144 |
| HpaI | GTTAAC | 1 cut(s) | 1972 |
| HpaII | CCGG | 4 cut(s) | 446, 516, 924, 1417 |
| HphI | GGTGA | 2 cut(s) | 354, 1822 |
| Hpy188I | TCNGA | 6 cut(s) | 199, 280, 454, 563, 895, 2001 |
| Hpy188III | TCNNGA | 8 cut(s) | 155, 428, 1052, 1629, 1678, 1914, 1948, 2276 |
| HpyAV | CCTTC | 5 cut(s) | 326, 384, 996, 1752, 1989 |
| HpyCH4IV | ACGT | 1 cut(s) | 1968 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 599, 708, 746, 755, 1304, 1490 |
| HpyF3I | CTNAG | 7 cut(s) | 32, 892, 967, 1445, 2000, 2049, 2259 |
| HpySE526I | ACGT | 1 cut(s) | 1968 |
| HspAI | GCGC | 2 cut(s) | 1556, 1824 |
| KspAI | GTTAAC | 1 cut(s) | 1972 |
| Kzo9I | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| LmnI | GCTCC | 3 cut(s) | 563, 745, 2042 |
| LweI | GCATC | 3 cut(s) | 628, 1327, 1696 |
| MaeI | CTAG | 7 cut(s) | 336, 633, 1506, 1574, 1724, 1841, 1995 |
| MaeII | ACGT | 1 cut(s) | 1968 |
| MaeIII | GTNAC | 5 cut(s) | 215, 1654, 1984, 2029, 2189 |
| MalI | GATC | 4 cut(s) | 1022, 1050, 1763, 1767 |
| MboI | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| MboII | GAAGA | 4 cut(s) | 880, 1018, 1370, 2113 |
| MfeI | CAATTG | 4 cut(s) | 867, 1461, 2070, 2091 |
| MhlI | GDGCHC | 5 cut(s) | 568, 742, 968, 1451, 2047 |
| MlsI | TGGCCA | 1 cut(s) | 758 |
| MluNI | TGGCCA | 1 cut(s) | 758 |
| MlyI | GAGTC | 4 cut(s) | 266, 458, 1169, 2060 |
| MmeI | TCCRAC | 3 cut(s) | 755, 1204, 2020 |
| Mox20I | TGGCCA | 1 cut(s) | 758 |
| Mph1103I | ATGCAT | 2 cut(s) | 1373, 1689 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1362 |
| MscI | TGGCCA | 1 cut(s) | 758 |
| MseI | TTAA | 3 cut(s) | 137, 1206, 1971 |
| MslI | CAYNNNNRTG | 4 cut(s) | 173, 498, 1250, 2280 |
| Msp20I | TGGCCA | 1 cut(s) | 758 |
| MspA1I | CMGCKG | 3 cut(s) | 677, 806, 971 |
| MspI | CCGG | 4 cut(s) | 446, 516, 924, 1417 |
| MspR9I | CCNGG | 5 cut(s) | 288, 517, 1097, 1560, 1836 |
| MunI | CAATTG | 4 cut(s) | 867, 1461, 2070, 2091 |
| Mva1269I | GAATGC | 1 cut(s) | 1689 |
| MvaI | CCWGG | 4 cut(s) | 288, 1097, 1560, 1836 |
| MvnI | CGCG | 2 cut(s) | 1556, 1604 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 599, 708, 746, 755, 1304, 1490 |
| NciI | CCSGG | 1 cut(s) | 517 |
| NcoI | CCATGG | 1 cut(s) | 1044 |
| NdeII | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| NlaIV | GGNNCC | 4 cut(s) | 565, 928, 1512, 2044 |
| NmeAIII | GCCGAG | 1 cut(s) | 619 |
| NmuCI | GTSAC | 2 cut(s) | 1654, 1984 |
| NsiI | ATGCAT | 2 cut(s) | 1373, 1689 |
| NspI | RCATGY | 2 cut(s) | 1941, 2231 |
| NspV | TTCGAA | 1 cut(s) | 1004 |
| OliI | CACNNNNGTG | 1 cut(s) | 173 |
| PaeI | GCATGC | 1 cut(s) | 2231 |
| PagI | TCATGA | 2 cut(s) | 154, 2275 |
| PceI | AGGCCT | 1 cut(s) | 474 |
| PciI | ACATGT | 1 cut(s) | 1937 |
| PctI | GAATGC | 1 cut(s) | 1689 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1362 |
| PfeI | GAWTC | 8 cut(s) | 146, 300, 455, 512, 1193, 1327, 1813, 2239 |
| PinAI | ACCGGT | 1 cut(s) | 923 |
| PleI | GAGTC | 4 cut(s) | 265, 457, 1168, 2059 |
| PpsI | GAGTC | 4 cut(s) | 265, 457, 1168, 2059 |
| PscI | ACATGT | 1 cut(s) | 1937 |
| Psp6I | CCWGG | 4 cut(s) | 286, 1095, 1558, 1834 |
| PspGI | CCWGG | 4 cut(s) | 286, 1095, 1558, 1834 |
| PspN4I | GGNNCC | 4 cut(s) | 565, 928, 1512, 2044 |
| PspPI | GGNCC | 5 cut(s) | 479, 709, 983, 1511, 1731 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 2174, 2206 |
| PstI | CTGCAG | 1 cut(s) | 676 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1835 |
| PvuII | CAGCTG | 3 cut(s) | 677, 806, 971 |
| RsaI | GTAC | 7 cut(s) | 93, 297, 655, 724, 1396, 1895, 2089 |
| RsaNI | GTAC | 7 cut(s) | 92, 296, 654, 723, 1395, 1894, 2088 |
| RseI | CAYNNNNRTG | 4 cut(s) | 173, 498, 1250, 2280 |
| SaqAI | TTAA | 3 cut(s) | 137, 1206, 1971 |
| Sau3AI | GATC | 4 cut(s) | 1020, 1048, 1761, 1765 |
| Sau96I | GGNCC | 5 cut(s) | 479, 709, 983, 1511, 1731 |
| SchI | GAGTC | 4 cut(s) | 266, 458, 1169, 2060 |
| ScrFI | CCNGG | 5 cut(s) | 288, 517, 1097, 1560, 1836 |
| SduI | GDGCHC | 5 cut(s) | 568, 742, 968, 1451, 2047 |
| SfaNI | GCATC | 3 cut(s) | 628, 1327, 1696 |
| SfcI | CTRYAG | 2 cut(s) | 672, 1215 |
| SfuI | TTCGAA | 1 cut(s) | 1004 |
| SinI | GGWCC | 2 cut(s) | 983, 1511 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 173, 498, 1250, 2280 |
| SmlI | CTYRAG | 3 cut(s) | 10, 664, 1538 |
| SmoI | CTYRAG | 3 cut(s) | 10, 664, 1538 |
| SpeI | ACTAGT | 1 cut(s) | 1840 |
| SphI | GCATGC | 1 cut(s) | 2231 |
| SseBI | AGGCCT | 1 cut(s) | 474 |
| SsiI | CCGC | 3 cut(s) | 735, 1554, 1604 |
| SspI | AATATT | 1 cut(s) | 382 |
| SspMI | CTAG | 7 cut(s) | 336, 633, 1506, 1574, 1724, 1841, 1995 |
| StuI | AGGCCT | 1 cut(s) | 474 |
| StyD4I | CCNGG | 5 cut(s) | 286, 515, 1095, 1558, 1834 |
| StyI | CCWWGG | 3 cut(s) | 712, 1044, 1485 |
| TaiI | ACGT | 1 cut(s) | 1971 |
| TaqI | TCGA | 5 cut(s) | 1004, 1051, 1399, 1764, 1915 |
| TatI | WGTACW | 1 cut(s) | 91 |
| TauI | GCSGC | 1 cut(s) | 1556 |
| TfiI | GAWTC | 8 cut(s) | 146, 300, 455, 512, 1193, 1327, 1813, 2239 |
| Tru1I | TTAA | 3 cut(s) | 137, 1206, 1971 |
| Tru9I | TTAA | 3 cut(s) | 137, 1206, 1971 |
| TscAI | CASTG | 4 cut(s) | 175, 676, 867, 2191 |
| TseFI | GTSAC | 2 cut(s) | 1654, 1984 |
| Tsp45I | GTSAC | 2 cut(s) | 1654, 1984 |
| TspDTI | ATGAA | 7 cut(s) | 308, 480, 923, 1371, 1587, 1776, 2195 |
| TspGWI | ACGGA | 2 cut(s) | 1972, 2235 |
| TspRI | CASTG | 4 cut(s) | 175, 676, 867, 2191 |
| VpaK11BI | GGWCC | 2 cut(s) | 983, 1511 |
| XapI | RAATTY | 4 cut(s) | 620, 1055, 1401, 1783 |
| XceI | RCATGY | 2 cut(s) | 1941, 2231 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 397 |
| XmiI | GTMKAC | 4 cut(s) | 210, 1152, 1421, 1845 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1362 |
| XspI | CTAG | 7 cut(s) | 336, 633, 1506, 1574, 1724, 1841, 1995 |
| Zsp2I | ATGCAT | 2 cut(s) | 1373, 1689 |