pycom14g11470

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001127
Physical Location & Seq
Forward (+)
14557842 .. 14563380
5539 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3165 bp
ATGGGCTTCCTTTGGCTGAAAGGTAAGAGCAACAAGGCTTTACTACTTGTGGCAGTTAGTTTTCTGTTAGTTTTCAATGGCAAGAACACTCTGATTGCACCAGCTGATGCCAAGAGTAGACTGCACATAGTTTATTTGGGTGAGAAGCAGCATGAGGATCCTGAGCTTTTAACCACTTCACATCATGAGATGTTGGCATCAGTTGTTGGCAGCAAAGAAGCCGCCATTGATTCAATGGTATATAGCTATAGACACGGCTTCTCTGGCTTTGCAGCCAAGCTTACAGAATCGCAAGCCAAGAAGATTTCAGAGTTTTCTGGAGTTGTTCATGTCACGCCAAATAGCTTTTACTCACCACAAACTACTAGGAGTTGGGATTACCTAGGCCTCTCTTCTCATTCCCCCACCAATTTGATGCACGAGACCAACATGGGCGATGGGATTGTCATTGGCATCATGGACTTCGGAATATGGCCAGAGTCGAAAATGCTAAATGATGATGGGGTTGGGCCAATCCCTACCCGTTGGAAGGGCCGATGTGAATCAGGAGAGAGCTTCAATGCCACAACACACTGCAACAGAAAACTTATAGGAGCAAAGTGGTTCATAGATGGCTTCCTTGCTGACAATGAGCAACCATTCAACACAGTATTTTGGGAGTTCTATTCTCCGAGAGATGGAGCAGGACACGGAACACACACTGCCACAACCGCTGCTGGTTCCTTTGTGGCCAATGCAAGTTACCAAGGGCTTGCCCTAGGAGTAACATGTTGGAATGTGCTAAATTTCGTGTGCGCTGCTGCTGATCTGCTTAAAGCTTTTGATGAAGCAATATATGATGGTGTAGATGTATTGTCGCTGTCTATTGGCAACTACAATCCTAAGTTTGCAGAGGTTGATAAGCGTGACGCGATTGCCACAGGGTCATTCCATGCTGTGGCCAAAAGTATTACTGTTGTTTGTGCTGCAGACAATACTGGACCTGCTCCTCAGACAGTGTCGAATGTAGCACCCTGGATCATAACCATGGCAGCTACCACCATCGACAGATCCTTCCCCACTCCCATTACTCTCGGGAATAACAAAACTCTACTGGGGCAAGCCATGTTTGTAGGAAAAGAGGTTGGTTTCACTGGTTTGGTGTATCCAAAAGGCCCAGAACAATTCCCTACAACATATGGTGTCTGCGAGTCCTTGACACTTAATACCACACATGTGGCTGGAAATGTAGTCATTTGCTTCACAACGATGCCTGGGCCAGCACAAGTAACCAGTGTTGTATCTGCTGTTAGAAGTGCAGGCGGAGTCGGGGTGATTATTGCAAGGAATCCTAGTAATCTCTTCGGTCCTTGCAGCAATGACTTCCCCTGCATTGTAGTTGACTACAAACTTGGCACCGAGATAATGTTATATATTCGTTCCACAAGATCTCCTACTGTAAAACTGAGCCCTTCTAAGACACTAACGGGGAAGCCTATATCGACTAAAGTTGCTTACTTCTCTTCTAGAGGACCTAATGCCATCGCTCCTGCAATTTTGAAGCCAGATATAGCCGCGCCTGGTGTGAACATATTAGCAGCCAGTTCGTATGATCCCGCTATGGATGGAAGATTTGCCTTGCTATCGGGGACATCAATGGCAACTCCCCACATTGCAGGCATTGTGGCAATTTTAAAATCATCGCACCCTGGTTGGTCCCCTGCTGCAATGAAATCAGCACTAGTCATAACAGCATGGAAGACTGATCCGTTTGGAGAGCCAATCTTTGCTGAAGGGACAGGGCAAAAGTTTGCGGATCCATTTGACTATGGAGGAGGATTAGTGAACCCGAACAAGGAAGCGAATCCTGGCCTCATATATGACATGGGCACAAATGACTACATAAACTACCTTTGTGCGTTTGGCTACAACACCTCAGCTATTTCTCTGCTTGTTAAACAAGCAACATCATGTCCTGTTATAAAACCTTCAATTCTTGATGTGAACCTGCCTTCCATTACAATTCCAAATCTAAGAAGTCCTGTAACACTCACTCGAACTGTAACGAATGTTGGCCCTGTCATCTCAACATACAAAGCCCAAATCGAGCCTCCACCAGGCATCAATGTAGTTGTGAAGCCAGAGGCATTGGTCTTCAACTCGACAGTTAAGGCACTCTCCTTTACAGTTGCGGTTTCCACCACCTACCAAGTGAACATGGCATACTTTTTCGGAAGCCTGACATGGACTGATGGGGTGCATGCTGTGTCAAGTCCCATATCTGTGAGAACACAAATCATACAATCCTATACTGATGACAACCATAGGGATCCTGAGCTTTTAACCATTTCACATCATGAGATGCTGGCATCAGTTGTTGGCAGTAAATATCTAAAATGGTTGCCAATTATGTGTGGTTTAATCATATGTCACAGCAAAGAAGCCGCCATTGACTCAATGGTCTATAGCTATAGACAAGGCTTCTCTGGCTTCGCAACCAAGCTTACAGAATCGCAAGCAAGGAGGATTTCAGAGTTCTCTGGAGTTGTTCATGTCATGCCAAATACCTTTTACACACCGCAAACTACTAGGAGTTGGGATTACCTAGGCCTCTCTTCTCATTCCCCCACCAATGTTATGCATGAGACCAACATGGGAGATGGGATTGTCATTGGCATCATTGACAGCCGAATAGGGCCAGAGTTGAAAATGCTAATTGATGAGAGGGTTGGGCCAATCCCTACCCGTTGGAAGGGCCGATGTGAATCAGGAGAGCGCTTCAATGCCACAACAGACTGCAACATAAAACTTACAGGAGCAAAGTGGTTCATAGATGCATTCCTTGCTGACAATGAGCAACCATTCAACAATACAGAATTTCCGGAGTTCTTGTGTCCGAGAGATGCTGAAGGACACGAAAGACATGGAACGCACACTGTTACAACCGCTGCTAGTTCCCTTGTGGCCAATGCAAGTTGCAAAGGGCTTGCCCTAGGATTAGTCCGAGGTGGTGCACCACGGCTCACTTGGCCGTGTACAAGAGCTACTGATGAAGCAATAAATGATGGTGTAGATGCATTGTCGCTCTTTATTAGCCCCAACAATCCTAATCCTATGTTTGAGGTTGATAAGCGCGACACAAAAGCCATTGGCTTATTCCATGCTGTGGCCAAAGGTATTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

1055

Amino Acids

113.49

Weight (kDa)

6.54

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 42 - 118 2.9e-18 Peptidase inhibitor I9
Peptidase_S8 PF00082 145 - 582 5.5e-36 Subtilase family
fn3_6 PF17766 660 - 755 7.2e-28 Fibronectin type-III domain
Inhibitor_I9 PF05922 777 - 852 5.4e-11 Peptidase inhibitor I9
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1961
AasI GACNNNNNNGTC 1 cut(s) 2438
Acc36I ACCTGC 2 cut(s) 991, 1995
AccB1I GGYRCC 1 cut(s) 1394
AccB7I CCANNNNNTGG 2 cut(s) 937, 3145
AccI GTMKAC 1 cut(s) 118
AccII CGCG 3 cut(s) 911, 1556, 3114
AccIII TCCGGA 1 cut(s) 2860
AcoI YGGCCR 6 cut(s) 473, 729, 939, 2943, 3008, 3147
AcsI RAATTY 2 cut(s) 784, 2855
AcuI CTGAAG 2 cut(s) 1791, 2907
AfaI GTAC 1 cut(s) 3016
AfeI AGCGCT 1 cut(s) 2756
AfiI CCNNNNNNNGG 7 cut(s) 529, 677, 937, 1834, 2096, 2731, 3145
AflIII ACRYGT 2 cut(s) 767, 1213
AhlI ACTAGT 1 cut(s) 1720
AjnI CCWGG 6 cut(s) 1013, 1252, 1558, 1687, 1846, 2095
AleI CACNNNNGTG 1 cut(s) 1214
AloI GAACNNNNNNTCC 2 cut(s) 1976, 2008
Alw21I GWGCWC 1 cut(s) 2995
Alw26I GTCTC 2 cut(s) 416, 2618
Alw44I GTGCAC 1 cut(s) 2991
Ama87I CYCGRG 1 cut(s) 1073
Aor13HI TCCGGA 1 cut(s) 2860
Aor51HI AGCGCT 1 cut(s) 2756
ApaLI GTGCAC 1 cut(s) 2991
ApoI RAATTY 2 cut(s) 784, 2855
AspA2I CCTAGG 4 cut(s) 382, 757, 2584, 2971
AspLEI GCGC 4 cut(s) 797, 1558, 2757, 3114
AsuHPI GGTGA 3 cut(s) 152, 345, 1324
AvaI CYCGRG 1 cut(s) 1073
AvaII GGWCC 4 cut(s) 980, 1346, 1511, 1695
AvrII CCTAGG 4 cut(s) 382, 757, 2584, 2971
BaeGI GKGCMC 2 cut(s) 1871, 2995
BalI TGGCCA 5 cut(s) 475, 731, 941, 2945, 3149
BamHI GGATCC 3 cut(s) 157, 1795, 2308
BanI GGYRCC 1 cut(s) 1394
BanII GRGCYC 1 cut(s) 1451
BauI CACGAG 1 cut(s) 419
BbsI GAAGAC 2 cut(s) 1745, 2125
Bbv12I GWGCWC 1 cut(s) 2995
BbvCI CCTCAGC 1 cut(s) 1915
BceAI ACGGC 3 cut(s) 271, 2995, 3014
BcgI CGANNNNNNTGC 2 cut(s) 1566, 1600
BciT130I CCWGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
BciVI GTATCC 1 cut(s) 1155
BcoDI GTCTC 2 cut(s) 416, 2618
BcuI ACTAGT 1 cut(s) 1720
BfmI CTRYAG 4 cut(s) 247, 966, 2443, 2449
BfoI RGCGCY 1 cut(s) 2758
BfuAI ACCTGC 2 cut(s) 991, 1995
BfuI GTATCC 1 cut(s) 1155
BglII AGATCT 1 cut(s) 1427
BlnI CCTAGG 4 cut(s) 382, 757, 2584, 2971
Bme1390I CCNGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
Bme18I GGWCC 4 cut(s) 980, 1346, 1511, 1695
BmeT110I CYCGRG 1 cut(s) 1073
BmiI GGNNCC 6 cut(s) 159, 721, 1396, 1697, 1797, 2310
BmrFI CCNGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
BmrI ACTGGG 1 cut(s) 1103
BmuI ACTGGG 1 cut(s) 1103
BpiI GAAGAC 2 cut(s) 1745, 2125
BpmI CTGGAG 2 cut(s) 339, 2541
Bpu10I CCTNAGC 3 cut(s) 162, 1915, 2313
BsaBI GATNNNNATC 2 cut(s) 541, 2743
BsaI GGTCTC 2 cut(s) 416, 2618
BsaWI WCCGGW 1 cut(s) 2860
BsaXI ACNNNNNCTCC 8 cut(s) 1296, 1326, 1414, 1444, 1806, 1836, 2855, 2885
Bsc4I CCNNNNNNNGG 7 cut(s) 529, 677, 937, 1834, 2096, 2731, 3145
Bse1I ACTGG 5 cut(s) 982, 1098, 1138, 1272, 1581
Bse3DI GCAATG 3 cut(s) 1363, 1650, 1713
Bse8I GATNNNNATC 2 cut(s) 541, 2743
BseAI TCCGGA 1 cut(s) 2860
BseBI CCWGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
BseGI GGATG 1 cut(s) 1609
BseJI GATNNNNATC 2 cut(s) 541, 2743
BseLI CCNNNNNNNGG 7 cut(s) 529, 677, 937, 1834, 2096, 2731, 3145
BseMI GCAATG 3 cut(s) 1363, 1650, 1713
BseMII CTCAG 5 cut(s) 153, 1004, 1436, 1929, 2304
BseNI ACTGG 5 cut(s) 982, 1098, 1138, 1272, 1581
BseRI GAGGAG 2 cut(s) 978, 1827
BseSI GKGCMC 2 cut(s) 1871, 2995
BsgI GTGCAG 2 cut(s) 107, 1317
Bsh1236I CGCG 3 cut(s) 911, 1556, 3114
BshNI GGYRCC 1 cut(s) 1394
BsiHKAI GWGCWC 1 cut(s) 2995
BsiHKCI CYCGRG 1 cut(s) 1073
BsiSI CCGG 1 cut(s) 2861
BslFI GGGAC 4 cut(s) 1642, 1681, 1789, 2238
BslI CCNNNNNNNGG 7 cut(s) 529, 677, 937, 1834, 2096, 2731, 3145
BsmAI GTCTC 2 cut(s) 416, 2618
BsmFI GGGAC 4 cut(s) 1642, 1681, 1789, 2238
BsmI GAATGC 1 cut(s) 2816
Bso31I GGTCTC 2 cut(s) 416, 2618
BsoBI CYCGRG 1 cut(s) 1073
Bsp1286I GDGCHC 3 cut(s) 1451, 1871, 2995
Bsp13I TCCGGA 1 cut(s) 2860
Bsp1407I TGTACA 1 cut(s) 3014
Bsp143I GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
Bsp19I CCATGG 1 cut(s) 1026
BspCNI CTCAG 5 cut(s) 154, 1003, 1437, 1928, 2305
BspEI TCCGGA 1 cut(s) 2860
BspFNI CGCG 3 cut(s) 911, 1556, 3114
BspHI TCATGA 2 cut(s) 184, 2335
BspLI GGNNCC 6 cut(s) 159, 721, 1396, 1697, 1797, 2310
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 2 cut(s) 991, 1995
BspT107I GGYRCC 1 cut(s) 1394
BspTNI GGTCTC 2 cut(s) 416, 2618
BsrDI GCAATG 3 cut(s) 1363, 1650, 1713
BsrGI TGTACA 1 cut(s) 3014
BsrI ACTGG 5 cut(s) 982, 1098, 1138, 1272, 1581
BssMI GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
BssSI CACGAG 1 cut(s) 419
BssT1I CCWWGG 6 cut(s) 382, 745, 757, 1026, 2584, 2971
Bst2BI CACGAG 1 cut(s) 419
Bst2UI CCWGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
Bst4CI ACNGT 8 cut(s) 649, 955, 997, 1438, 2041, 2146, 2167, 2917
Bst6I CTCTTC 4 cut(s) 397, 1346, 1507, 2599
BstAPI GCANNNNNTGC 1 cut(s) 2822
BstAUI TGTACA 1 cut(s) 3014
BstDEI CTNAG 8 cut(s) 162, 882, 990, 1445, 1455, 1915, 2012, 2313
BstDSI CCRYGG 2 cut(s) 1026, 2996
BstENI CCTNNNNNAGG 1 cut(s) 2094
BstF5I GGATG 1 cut(s) 1609
BstFNI CGCG 3 cut(s) 911, 1556, 3114
BstH2I RGCGCY 1 cut(s) 2758
BstHHI GCGC 4 cut(s) 797, 1558, 2757, 3114
BstKTI GATC 9 cut(s) 160, 808, 1020, 1052, 1430, 1594, 1747, 1798, 2311
BstMAI GTCTC 2 cut(s) 416, 2618
BstMBI GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
BstMWI GCNNNNNNNGC 5 cut(s) 264, 710, 2466, 2822, 3007
BstNI CCWGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
BstNSI RCATGY 3 cut(s) 771, 1217, 2243
BstSCI CCNGG 6 cut(s) 1013, 1252, 1558, 1687, 1846, 2095
BstSFI CTRYAG 4 cut(s) 247, 966, 2443, 2449
BstSLI GKGCMC 2 cut(s) 1871, 2995
BstUI CGCG 3 cut(s) 911, 1556, 3114
BstV2I GAAGAC 2 cut(s) 1745, 2125
BstX2I RGATCY 5 cut(s) 157, 1049, 1427, 1795, 2308
BstXI CCANNNNNNTGG 1 cut(s) 1216
BstYI RGATCY 5 cut(s) 157, 1049, 1427, 1795, 2308
BsuI GTATCC 1 cut(s) 1155
BtgI CCRYGG 2 cut(s) 1026, 2996
BtgZI GCGATG 3 cut(s) 450, 1507, 1665
BtsCI GGATG 1 cut(s) 1609
BtsI GCAGTG 2 cut(s) 571, 699
BtsIMutI CAGTG 6 cut(s) 571, 699, 1002, 1131, 1279, 2913
BveI ACCTGC 2 cut(s) 991, 1995
CciI TCATGA 2 cut(s) 184, 2335
CfoI GCGC 4 cut(s) 797, 1558, 2757, 3114
CseI GACGC 1 cut(s) 917
Csp6I GTAC 1 cut(s) 3015
CviQI GTAC 1 cut(s) 3015
DdeI CTNAG 8 cut(s) 162, 882, 990, 1445, 1455, 1915, 2012, 2313
DpnI GATC 9 cut(s) 159, 807, 1019, 1051, 1429, 1593, 1746, 1797, 2310
DpnII GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
DraI TTTAAA 1 cut(s) 1674
DrdI GACNNNNNNGTC 1 cut(s) 2438
DseDI GACNNNNNNGTC 1 cut(s) 2438
EaeI YGGCCR 6 cut(s) 473, 729, 939, 2943, 3008, 3147
Eam1104I CTCTTC 4 cut(s) 397, 1346, 1507, 2599
EarI CTCTTC 4 cut(s) 397, 1346, 1507, 2599
EciI GGCGGA 1 cut(s) 1317
Eco130I CCWWGG 6 cut(s) 382, 745, 757, 1026, 2584, 2971
Eco147I AGGCCT 2 cut(s) 387, 2589
Eco24I GRGCYC 1 cut(s) 1451
Eco31I GGTCTC 2 cut(s) 416, 2618
Eco47I GGWCC 4 cut(s) 980, 1346, 1511, 1695
Eco47III AGCGCT 1 cut(s) 2756
Eco57I CTGAAG 2 cut(s) 1791, 2907
Eco88I CYCGRG 1 cut(s) 1073
EcoNI CCTNNNNNAGG 1 cut(s) 2094
EcoO109I RGGNCCY 1 cut(s) 1511
EcoRII CCWGG 6 cut(s) 1013, 1252, 1558, 1687, 1846, 2095
EcoT14I CCWWGG 6 cut(s) 382, 745, 757, 1026, 2584, 2971
EcoT22I ATGCAT 3 cut(s) 2622, 2818, 3058
EcoT38I GRGCYC 1 cut(s) 1451
ErhI CCWWGG 6 cut(s) 382, 745, 757, 1026, 2584, 2971
FaqI GGGAC 4 cut(s) 1642, 1681, 1789, 2238
FauI CCCGC 1 cut(s) 1603
FauNDI CATATG 2 cut(s) 1177, 2405
FblI GTMKAC 1 cut(s) 118
FokI GGATG 1 cut(s) 1616
FriOI GRGCYC 1 cut(s) 1451
GlaI GCGC 4 cut(s) 796, 1557, 2756, 3113
GsuI CTGGAG 2 cut(s) 339, 2541
HaeII RGCGCY 1 cut(s) 2758
HapII CCGG 1 cut(s) 2861
HgaI GACGC 1 cut(s) 917
HhaI GCGC 4 cut(s) 797, 1558, 2757, 3114
Hin6I GCGC 4 cut(s) 795, 1556, 2755, 3112
HinP1I GCGC 4 cut(s) 795, 1556, 2755, 3112
HincII GTYRAC 1 cut(s) 1381
HindII GTYRAC 1 cut(s) 1381
HindIII AAGCTT 3 cut(s) 278, 816, 2480
HpaII CCGG 1 cut(s) 2861
HphI GGTGA 3 cut(s) 152, 345, 1324
Hpy166II GTNNAC 8 cut(s) 119, 1381, 1567, 1825, 1984, 2194, 2993, 3015
Hpy188I TCNGA 9 cut(s) 93, 310, 467, 672, 993, 2213, 2512, 2877, 2984
Hpy8I GTNNAC 8 cut(s) 119, 1381, 1567, 1825, 1984, 2194, 2993, 3015
HpyAV CCTTC 8 cut(s) 523, 1063, 1461, 1766, 1977, 2001, 2725, 2882
HpyCH4III ACNGT 8 cut(s) 649, 955, 997, 1438, 2041, 2146, 2167, 2917
HpyF10VI GCNNNNNNNGC 5 cut(s) 264, 710, 2466, 2822, 3007
HpyF3I CTNAG 8 cut(s) 162, 882, 990, 1445, 1455, 1915, 2012, 2313
HspAI GCGC 4 cut(s) 795, 1556, 2755, 3112
Kpn2I TCCGGA 1 cut(s) 2860
Kzo9I GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
LmnI GCTCC 5 cut(s) 593, 680, 991, 1531, 2795
MaeIII GTNAC 9 cut(s) 331, 740, 763, 905, 1267, 2023, 2041, 2408, 2917
MalI GATC 9 cut(s) 159, 807, 1019, 1051, 1429, 1593, 1746, 1797, 2310
MboI GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
MboII GAAGA 8 cut(s) 313, 384, 1333, 1494, 1620, 1750, 2125, 2586
MflI RGATCY 5 cut(s) 157, 1049, 1427, 1795, 2308
MhlI GDGCHC 3 cut(s) 1451, 1871, 2995
MlsI TGGCCA 5 cut(s) 475, 731, 941, 2945, 3149
MluNI TGGCCA 5 cut(s) 475, 731, 941, 2945, 3149
MlyI GAGTC 4 cut(s) 488, 1199, 1314, 2426
MmeI TCCRAC 3 cut(s) 506, 752, 2708
Mox20I TGGCCA 5 cut(s) 475, 731, 941, 2945, 3149
Mph1103I ATGCAT 3 cut(s) 2622, 2818, 3058
MroI TCCGGA 1 cut(s) 2860
MscI TGGCCA 5 cut(s) 475, 731, 941, 2945, 3149
MseI TTAA 8 cut(s) 170, 813, 1203, 1673, 1935, 2148, 2321, 2399
MslI CAYNNNNRTG 4 cut(s) 1025, 1214, 1247, 2261
Msp20I TGGCCA 5 cut(s) 475, 731, 941, 2945, 3149
MspA1I CMGCKG 3 cut(s) 104, 713, 2927
MspI CCGG 1 cut(s) 2861
MspR9I CCNGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
Mva1269I GAATGC 1 cut(s) 2816
MvaI CCWGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
MvnI CGCG 3 cut(s) 911, 1556, 3114
MwoI GCNNNNNNNGC 5 cut(s) 264, 710, 2466, 2822, 3007
NcoI CCATGG 1 cut(s) 1026
NdeI CATATG 2 cut(s) 1177, 2405
NdeII GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
NlaIV GGNNCC 6 cut(s) 159, 721, 1396, 1697, 1797, 2310
NmuCI GTSAC 3 cut(s) 331, 905, 2408
NsiI ATGCAT 3 cut(s) 2622, 2818, 3058
NspI RCATGY 3 cut(s) 771, 1217, 2243
OliI CACNNNNGTG 1 cut(s) 1214
PaeI GCATGC 1 cut(s) 2243
PagI TCATGA 2 cut(s) 184, 2335
PceI AGGCCT 2 cut(s) 387, 2589
PciI ACATGT 2 cut(s) 767, 1213
PctI GAATGC 1 cut(s) 2816
PfeI GAWTC 7 cut(s) 230, 287, 542, 1327, 1843, 2489, 2744
PflFI GACNNNGTC 1 cut(s) 997
PflMI CCANNNNNTGG 2 cut(s) 937, 3145
PleI GAGTC 4 cut(s) 487, 1198, 1313, 2426
PpsI GAGTC 4 cut(s) 487, 1198, 1313, 2426
PpuMI RGGWCCY 1 cut(s) 1511
PscI ACATGT 2 cut(s) 767, 1213
PsiI TTATAA 1 cut(s) 1961
Psp5II RGGWCCY 1 cut(s) 1511
Psp6I CCWGG 6 cut(s) 1013, 1252, 1558, 1687, 1846, 2095
PspGI CCWGG 6 cut(s) 1013, 1252, 1558, 1687, 1846, 2095
PspN4I GGNNCC 6 cut(s) 159, 721, 1396, 1697, 1797, 2310
PspPPI RGGWCCY 1 cut(s) 1511
PsrI GAACNNNNNNTAC 2 cut(s) 2186, 2218
PstI CTGCAG 1 cut(s) 970
PsuI RGATCY 5 cut(s) 157, 1049, 1427, 1795, 2308
PsyI GACNNNGTC 1 cut(s) 997
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 1 cut(s) 3016
RsaNI GTAC 1 cut(s) 3015
RseI CAYNNNNRTG 4 cut(s) 1025, 1214, 1247, 2261
SaqAI TTAA 8 cut(s) 170, 813, 1203, 1673, 1935, 2148, 2321, 2399
Sau3AI GATC 9 cut(s) 157, 805, 1017, 1049, 1427, 1591, 1744, 1795, 2308
SchI GAGTC 4 cut(s) 488, 1199, 1314, 2426
ScrFI CCNGG 6 cut(s) 1015, 1254, 1560, 1689, 1848, 2097
SduI GDGCHC 3 cut(s) 1451, 1871, 2995
SfcI CTRYAG 4 cut(s) 247, 966, 2443, 2449
SinI GGWCC 4 cut(s) 980, 1346, 1511, 1695
SmiMI CAYNNNNRTG 4 cut(s) 1025, 1214, 1247, 2261
SpeI ACTAGT 1 cut(s) 1720
SphI GCATGC 1 cut(s) 2243
SseBI AGGCCT 2 cut(s) 387, 2589
StuI AGGCCT 2 cut(s) 387, 2589
StyD4I CCNGG 6 cut(s) 1013, 1252, 1558, 1687, 1846, 2095
StyI CCWWGG 6 cut(s) 382, 745, 757, 1026, 2584, 2971
TaaI ACNGT 8 cut(s) 649, 955, 997, 1438, 2041, 2146, 2167, 2917
TaqI TCGA 7 cut(s) 482, 1001, 1044, 1481, 2035, 2085, 2141
TaqII GACCGA 1 cut(s) 1334
TatI WGTACW 1 cut(s) 3014
TauI GCSGC 3 cut(s) 224, 1556, 2426
TfiI GAWTC 7 cut(s) 230, 287, 542, 1327, 1843, 2489, 2744
Tru1I TTAA 8 cut(s) 170, 813, 1203, 1673, 1935, 2148, 2321, 2399
Tru9I TTAA 8 cut(s) 170, 813, 1203, 1673, 1935, 2148, 2321, 2399
TscAI CASTG 6 cut(s) 578, 706, 1002, 1138, 1279, 2920
TseFI GTSAC 3 cut(s) 331, 905, 2408
Tsp45I GTSAC 3 cut(s) 331, 905, 2408
TspDTI ATGAA 7 cut(s) 317, 595, 840, 1724, 2519, 2797, 3045
TspGWI ACGGA 2 cut(s) 705, 1737
TspRI CASTG 6 cut(s) 578, 706, 1002, 1138, 1279, 2920
Tth111I GACNNNGTC 1 cut(s) 997
Van91I CCANNNNNTGG 2 cut(s) 937, 3145
VneI GTGCAC 1 cut(s) 2991
VpaK11BI GGWCC 4 cut(s) 980, 1346, 1511, 1695
XagI CCTNNNNNAGG 1 cut(s) 2094
XapI RAATTY 2 cut(s) 784, 2855
XbaI TCTAGA 1 cut(s) 1505
XceI RCATGY 3 cut(s) 771, 1217, 2243
XcmI CCANNNNNNNNNTGG 3 cut(s) 232, 2434, 3003
XmaJI CCTAGG 4 cut(s) 382, 757, 2584, 2971
XmiI GTMKAC 1 cut(s) 118
Zsp2I ATGCAT 3 cut(s) 2622, 2818, 3058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.