pycom15g07160

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003321
Physical Location & Seq
Forward (+)
4377163 .. 4378681
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 915 bp
ATGCTACAGAAACAAATCCACTGCTATAGTTTCACCACCACTAAATTAACAATTACTCTGCTGCTCGTCGTCCCAAGTAAAGTGAGACAAAAAACAAACCACATTGGCCATTGCAAAAATCAAATCATGGGCATGTCCAAATGGTTTTTGTTTCTCTATGTTTTACTATTAACCCAATTCAATGGGGCTGCTTCCGTTGCCAGGAACATCTTCATCCTAGCAGGTCAGAGCAACATGGCTGGGCGCGGGGGCGTCAGCCGCGGCAAATGGGACGGGAACGTCCCAACCGAGTGCAGTCCCAACCCATCAATCCTCCGGCTTAGTGCACCGCTCATATGGGAGCAAGCACACGAGCCGCTCCACGCCGACATCGACCTGGGAAAGACGTGTGGGATTGGGCCGGGCATGGCATTTGCCAATGAGGTCCTCCGAGCCAAGGGGTCGGGATTTGGGGAAGTGGGTCTGGTTCCTTGCGCCGTAGGCGGGACTAGAATTGGCGAGTGGGGTAAAGGGACTCGGTTGTATGGTGAGTTGGTGAGACGAGCCCGCGAGTCGGTCAGGGGCGGAGGAGTTATCCGGGCGCTTTTGTGGTTTCAGGGAGAAAGTGACACCGTGAGAAAGGAGGAAGCTGACGGGTATAAAGGGAAATTCGAGAAGCTTGTTTTGGACCTGCGTTCTGATCTTCATCTCCCTAACCTTCATGTCATCCAGGTGGCACTGGCATCTGGAGAAGGCAAATTTGTAGACGTAGTAAGGAAAGGTCAGCTGGATGTCAAGTTACCAAATGTGAAATGTGTTGATGCCAAAGGATTGCGTCTCAAAGAAGATGGCCTCCACCTTACTACGTTATCTGAAGTCCACTTGGGGATCAAGCTTGCTCGTTCCTTACTTGATTCTTATGGCCACCATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

305

Amino Acids

33.24

Weight (kDa)

9.51

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 68 - 297 3.2e-89 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
Acc36I ACCTGC 2 cut(s) 212, 678
AccBSI CCGCTC 2 cut(s) 331, 358
AccI GTMKAC 1 cut(s) 744
AccII CGCG 3 cut(s) 246, 261, 549
AciI CCGC 8 cut(s) 246, 259, 261, 329, 356, 483, 547, 564
AclWI GGATC 1 cut(s) 875
AcoI YGGCCR 2 cut(s) 106, 901
AcsI RAATTY 2 cut(s) 647, 737
AcuI CTGAAG 1 cut(s) 873
AcyI GRCGYC 1 cut(s) 252
AfiI CCNNNNNNNGG 4 cut(s) 201, 436, 483, 553
AflIII ACRYGT 1 cut(s) 386
AgsI TTSAA 1 cut(s) 181
AjiI CACGTC 1 cut(s) 387
AjnI CCWGG 3 cut(s) 200, 375, 708
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 4 cut(s) 629, 658, 766, 874
AluI AGCT 4 cut(s) 629, 658, 766, 874
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 3 cut(s) 79, 532, 821
Alw44I GTGCAC 1 cut(s) 324
AlwI GGATC 1 cut(s) 875
AoxI GGCC 4 cut(s) 106, 398, 829, 901
ApaLI GTGCAC 1 cut(s) 324
ApeKI GCWGC 2 cut(s) 61, 188
ApoI RAATTY 2 cut(s) 647, 737
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 3 cut(s) 246, 476, 583
AspS9I GGNCC 3 cut(s) 398, 424, 667
AsuC2I CCSGG 2 cut(s) 402, 578
AsuHPI GGTGA 3 cut(s) 25, 539, 547
AvaII GGWCC 2 cut(s) 424, 667
BaeGI GKGCMC 1 cut(s) 328
BalI TGGCCA 2 cut(s) 108, 903
BanII GRGCYC 1 cut(s) 547
BauI CACGAG 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 2 cut(s) 48, 175
BccI CCATC 2 cut(s) 313, 821
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 3 cut(s) 202, 377, 710
BcnI CCSGG 2 cut(s) 402, 578
BcoDI GTCTC 3 cut(s) 79, 532, 821
BfaI CTAG 2 cut(s) 218, 489
BfmI CTRYAG 2 cut(s) 5, 25
BfoI RGCGCY 1 cut(s) 584
BfuAI ACCTGC 2 cut(s) 212, 678
BisI GCNGC 5 cut(s) 62, 189, 259, 262, 356
BlsI GCNGC 5 cut(s) 63, 190, 260, 263, 357
Bme1390I CCNGG 5 cut(s) 202, 377, 402, 578, 710
Bme18I GGWCC 2 cut(s) 424, 667
BmgBI CACGTC 1 cut(s) 387
BmgT120I GGNCC 3 cut(s) 398, 424, 667
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 5 cut(s) 202, 377, 402, 578, 710
BmsI GCATC 2 cut(s) 731, 790
BpmI CTGGAG 1 cut(s) 747
BpuMI CCSGG 2 cut(s) 402, 578
BsaBI GATNNNNATC 1 cut(s) 684
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 3 cut(s) 259, 376, 435
Bsc4I CCNNNNNNNGG 4 cut(s) 201, 436, 483, 553
Bse1I ACTGG 1 cut(s) 723
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 684
BseBI CCWGG 3 cut(s) 202, 377, 710
BseDI CCNNGG 3 cut(s) 259, 376, 435
BseGI GGATG 3 cut(s) 213, 705, 775
BseJI GATNNNNATC 1 cut(s) 684
BseLI CCNNNNNNNGG 4 cut(s) 201, 436, 483, 553
BseMI GCAATG 1 cut(s) 109
BseNI ACTGG 1 cut(s) 723
BseRI GAGGAG 1 cut(s) 582
BseSI GKGCMC 1 cut(s) 328
BseXI GCAGC 2 cut(s) 48, 175
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 1 cut(s) 313
Bsh1236I CGCG 3 cut(s) 246, 261, 549
BshFI GGCC 4 cut(s) 108, 400, 831, 903
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 3 cut(s) 316, 401, 577
BslFI GGGAC 6 cut(s) 56, 266, 282, 284, 499, 526
BslI CCNNNNNNNGG 4 cut(s) 201, 436, 483, 553
BsmAI GTCTC 3 cut(s) 79, 532, 821
BsmBI CGTCTC 2 cut(s) 532, 821
BsmFI GGGAC 6 cut(s) 56, 266, 282, 284, 499, 526
BsnI GGCC 4 cut(s) 108, 400, 831, 903
Bsp1286I GDGCHC 2 cut(s) 328, 547
Bsp143I GATC 2 cut(s) 679, 867
BspACI CCGC 8 cut(s) 246, 259, 261, 329, 356, 483, 547, 564
BspANI GGCC 4 cut(s) 108, 400, 831, 903
BspFNI CGCG 3 cut(s) 246, 261, 549
BspLI GGNNCC 1 cut(s) 468
BspMI ACCTGC 2 cut(s) 212, 678
BspPI GGATC 1 cut(s) 875
BsrBI CCGCTC 2 cut(s) 331, 358
BsrDI GCAATG 1 cut(s) 109
BsrI ACTGG 1 cut(s) 723
BssECI CCNNGG 3 cut(s) 259, 376, 435
BssMI GATC 2 cut(s) 679, 867
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 350
Bst2UI CCWGG 3 cut(s) 202, 377, 710
Bst4CI ACNGT 1 cut(s) 613
BstACI GRCGYC 1 cut(s) 252
BstC8I GCNNGC 3 cut(s) 345, 547, 876
BstDEI CTNAG 1 cut(s) 320
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 3 cut(s) 213, 705, 775
BstFNI CGCG 3 cut(s) 246, 261, 549
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 3 cut(s) 246, 476, 583
BstKTI GATC 2 cut(s) 682, 870
BstMAI GTCTC 3 cut(s) 79, 532, 821
BstMBI GATC 2 cut(s) 679, 867
BstMWI GCNNNNNNNGC 3 cut(s) 197, 258, 480
BstNI CCWGG 3 cut(s) 202, 377, 710
BstNSI RCATGY 1 cut(s) 136
BstSCI CCNGG 5 cut(s) 200, 375, 400, 576, 708
BstSFI CTRYAG 2 cut(s) 5, 25
BstSLI GKGCMC 1 cut(s) 328
BstUI CGCG 3 cut(s) 246, 261, 549
BstV1I GCAGC 2 cut(s) 48, 175
BstXI CCANNNNNNTGG 1 cut(s) 182
BsuRI GGCC 4 cut(s) 108, 400, 831, 903
BtgI CCRYGG 1 cut(s) 259
BtrI CACGTC 1 cut(s) 387
BtsCI GGATG 3 cut(s) 213, 705, 775
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 2 cut(s) 19, 716
BveI ACCTGC 2 cut(s) 212, 678
Cac8I GCNNGC 3 cut(s) 345, 547, 876
CfoI GCGC 3 cut(s) 246, 476, 583
Cfr13I GGNCC 3 cut(s) 398, 424, 667
Cfr42I CCGCGG 1 cut(s) 262
CseI GACGC 2 cut(s) 241, 803
CspCI CAANNNNNGTGG 1 cut(s) 893
CviAII CATG 5 cut(s) 127, 133, 235, 406, 701
DdeI CTNAG 1 cut(s) 320
DpnI GATC 2 cut(s) 681, 869
DpnII GATC 2 cut(s) 679, 867
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 2 cut(s) 106, 901
EciI GGCGGA 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 547
Eco47I GGWCC 2 cut(s) 424, 667
Eco57I CTGAAG 1 cut(s) 873
EcoO109I RGGNCCY 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 200, 375, 708
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 435
Esp3I CGTCTC 2 cut(s) 532, 821
FaeI CATG 5 cut(s) 130, 136, 238, 409, 704
FaqI GGGAC 6 cut(s) 56, 266, 282, 284, 499, 526
FatI CATG 5 cut(s) 126, 132, 234, 405, 700
FauI CCCGC 3 cut(s) 239, 476, 554
FauNDI CATATG 1 cut(s) 335
FblI GTMKAC 1 cut(s) 744
Fnu4HI GCNGC 5 cut(s) 62, 189, 259, 262, 356
FokI GGATG 3 cut(s) 200, 692, 782
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 5 cut(s) 62, 189, 259, 262, 356
FspBI CTAG 2 cut(s) 218, 489
GlaI GCGC 3 cut(s) 245, 475, 582
GluI GCNGC 5 cut(s) 62, 189, 259, 262, 356
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 747
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 4 cut(s) 108, 400, 831, 903
HapII CCGG 3 cut(s) 316, 401, 577
HgaI GACGC 2 cut(s) 241, 803
HhaI GCGC 3 cut(s) 246, 476, 583
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 5 cut(s) 130, 136, 238, 409, 704
Hin6I GCGC 3 cut(s) 244, 474, 581
HinP1I GCGC 3 cut(s) 244, 474, 581
HindIII AAGCTT 2 cut(s) 656, 872
HinfI GANTC 3 cut(s) 514, 551, 893
HpaII CCGG 3 cut(s) 316, 401, 577
HphI GGTGA 3 cut(s) 25, 539, 547
Hpy166II GTNNAC 3 cut(s) 326, 745, 859
Hpy188I TCNGA 4 cut(s) 228, 431, 679, 853
Hpy188III TCNNGA 3 cut(s) 444, 652, 726
Hpy8I GTNNAC 3 cut(s) 326, 745, 859
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 707, 725
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 4 cut(s) 279, 386, 747, 845
HpyCH4V TGCA 3 cut(s) 114, 294, 326
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 258, 480
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 4 cut(s) 279, 386, 747, 845
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 5 cut(s) 130, 136, 238, 409, 704
HspAI GCGC 3 cut(s) 244, 474, 581
KspI CCGCGG 1 cut(s) 262
Kzo9I GATC 2 cut(s) 679, 867
LmnI GCTCC 2 cut(s) 340, 363
Lsp1109I GCAGC 2 cut(s) 48, 175
LweI GCATC 2 cut(s) 731, 790
MaeI CTAG 2 cut(s) 218, 489
MaeII ACGT 4 cut(s) 279, 386, 747, 845
MaeIII GTNAC 2 cut(s) 605, 777
MalI GATC 2 cut(s) 681, 869
MbiI CCGCTC 2 cut(s) 331, 358
MboI GATC 2 cut(s) 679, 867
MboII GAAGA 3 cut(s) 202, 674, 836
MhlI GDGCHC 2 cut(s) 328, 547
MlsI TGGCCA 2 cut(s) 108, 903
MluCI AATT 6 cut(s) 44, 51, 176, 492, 647, 737
MluNI TGGCCA 2 cut(s) 108, 903
MlyI GAGTC 2 cut(s) 508, 560
MnlI CCTC 6 cut(s) 323, 415, 437, 560, 616, 842
Mox20I TGGCCA 2 cut(s) 108, 903
MroXI GAANNNNTTC 1 cut(s) 209
MscI TGGCCA 2 cut(s) 108, 903
MseI TTAA 2 cut(s) 47, 170
MslI CAYNNNNRTG 2 cut(s) 131, 710
Msp20I TGGCCA 2 cut(s) 108, 903
MspA1I CMGCKG 2 cut(s) 261, 766
MspI CCGG 3 cut(s) 316, 401, 577
MspR9I CCNGG 5 cut(s) 202, 377, 402, 578, 710
MvaI CCWGG 3 cut(s) 202, 377, 710
MvnI CGCG 3 cut(s) 246, 261, 549
MwoI GCNNNNNNNGC 3 cut(s) 197, 258, 480
NciI CCSGG 2 cut(s) 402, 578
NdeI CATATG 1 cut(s) 335
NdeII GATC 2 cut(s) 679, 867
NlaIII CATG 5 cut(s) 130, 136, 238, 409, 704
NlaIV GGNNCC 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 605
NspI RCATGY 1 cut(s) 136
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 369
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 1 cut(s) 893
PkrI GCNGC 5 cut(s) 63, 190, 260, 263, 357
PleI GAGTC 2 cut(s) 508, 559
PpsI GAGTC 2 cut(s) 508, 559
PpuMI RGGWCCY 1 cut(s) 424
Psp5II RGGWCCY 1 cut(s) 424
Psp6I CCWGG 3 cut(s) 200, 375, 708
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 3 cut(s) 200, 375, 708
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 3 cut(s) 398, 424, 667
PspPPI RGGWCCY 1 cut(s) 424
PvuII CAGCTG 1 cut(s) 766
RseI CAYNNNNRTG 2 cut(s) 131, 710
SacII CCGCGG 1 cut(s) 262
SaqAI TTAA 2 cut(s) 47, 170
SatI GCNGC 5 cut(s) 62, 189, 259, 262, 356
Sau3AI GATC 2 cut(s) 679, 867
Sau96I GGNCC 3 cut(s) 398, 424, 667
SchI GAGTC 2 cut(s) 508, 560
ScrFI CCNGG 5 cut(s) 202, 377, 402, 578, 710
SduI GDGCHC 2 cut(s) 328, 547
SfaNI GCATC 2 cut(s) 731, 790
SfcI CTRYAG 2 cut(s) 5, 25
Sfr303I CCGCGG 1 cut(s) 262
SgrBI CCGCGG 1 cut(s) 262
SinI GGWCC 2 cut(s) 424, 667
SmiMI CAYNNNNRTG 2 cut(s) 131, 710
Sse9I AATT 6 cut(s) 44, 51, 176, 492, 647, 737
SsiI CCGC 8 cut(s) 246, 259, 261, 329, 356, 483, 547, 564
SspMI CTAG 2 cut(s) 218, 489
StyD4I CCNGG 5 cut(s) 200, 375, 400, 576, 708
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 4 cut(s) 282, 389, 750, 848
TaqI TCGA 2 cut(s) 372, 651
TaqII GACCGA 1 cut(s) 544
TasI AATT 6 cut(s) 44, 51, 176, 492, 647, 737
TauI GCSGC 3 cut(s) 261, 264, 358
TfiI GAWTC 1 cut(s) 893
Tru1I TTAA 2 cut(s) 47, 170
Tru9I TTAA 2 cut(s) 47, 170
TscAI CASTG 2 cut(s) 26, 723
TseFI GTSAC 1 cut(s) 605
TseI GCWGC 2 cut(s) 61, 188
Tsp45I GTSAC 1 cut(s) 605
TspDTI ATGAA 3 cut(s) 202, 674, 689
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 2 cut(s) 26, 723
VneI GTGCAC 1 cut(s) 324
VpaK11BI GGWCC 2 cut(s) 424, 667
XapI RAATTY 2 cut(s) 647, 737
XceI RCATGY 1 cut(s) 136
XmiI GTMKAC 1 cut(s) 744
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 2 cut(s) 218, 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.