pycom2575g00040

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00002575
OG0000037
Physical Location & Seq
Forward (+)
13466 .. 14833
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom2575g00040.4

Sequence Viewer

Length: 864 bp
ATGTGCAAGTGCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCCAGGTTTTGCGGCGACCGCCGCGCCCTCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTGTAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCATGCTCTCACTCGAACCCTTCTCAGAAGATCAAGGTCGGTCGGCGGTGCAACCCTCGTGGGGATCCCGCCAGTCAGCTTCCTTGCGCCTTACGGGTTTGATCGCCCGTTGACTCGCACGCATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGCCGAATGGGGAGCTCGCTGGCCGACGCCGGGAGCGCGCAGTTGCCGAAGCACGCCGGTACGGCCCCGGGCTTTGGCCGCCGCCGCAATCCGCGTCGGTCCACGCCCCGAGCCGATCGGCGGACCGGCTTTCGACCGTTCCACATCCGACCGGGGCGCATCGCCAGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACAGAATTTACCGGTCCGGGCACGACGGGGCTCTCACCCTCTCCGGCGCCACCTTCCAGTGGACTTGGGCCCGGTCCGCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

288

Amino Acids

32.2

Weight (kDa)

11.97

Isoelectric Point (pI)

72.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000037)

Species Orthologous Gene IDs
pyrus_communis pycom12555g00040 pycom12555g00090 pycom2132g00020 pycom2247g00020 pycom2303g00010 pycom2303g00070 pycom2303g00140 pycom2575g00040 pycom520g01330 pycom553g00030 pycom961g00020
rosa_laevigata RLG00000005153 RLG00000005811 RLG00000030826 RLG00000030829
rosa_multiflora Rmu_sc0001696.1_g000004
rosa_roxburghii Rroxscaffold_100G00451140 Rroxscaffold_100G00451150 Rroxscaffold_100G00451160 Rroxscaffold_102G00450910 Rroxscaffold_102G00450930 Rroxscaffold_103G00450540 Rroxscaffold_103G00450580 Rroxscaffold_103G00450590 Rroxscaffold_104G00444650 Rroxscaffold_104G00444700 Rroxscaffold_104G00444730 Rroxscaffold_104G00444770 Rroxscaffold_104G00444780 Rroxscaffold_104G00444820 Rroxscaffold_104G00444850 Rroxscaffold_104G00444890 Rroxscaffold_105G00447110 Rroxscaffold_105G00447150 Rroxscaffold_105G00447200 Rroxscaffold_105G00447210 Rroxscaffold_105G00447240 Rroxscaffold_105G00447250 Rroxscaffold_106G00451440 Rroxscaffold_106G00451450 Rroxscaffold_107G00442420 Rroxscaffold_107G00442430 Rroxscaffold_107G00442460 Rroxscaffold_107G00442490 Rroxscaffold_107G00442500 Rroxscaffold_107G00442530 Rroxscaffold_107G00442580 Rroxscaffold_107G00442610 Rroxscaffold_107G00442670 Rroxscaffold_108G00451460 Rroxscaffold_108G00451470 Rroxscaffold_110G00451500 Rroxscaffold_110G00451520 Rroxscaffold_111G00451570 Rroxscaffold_112G00451600 Rroxscaffold_113G00451610 Rroxscaffold_113G00451640 Rroxscaffold_114G00451660 Rroxscaffold_114G00451670 Rroxscaffold_115G00451700 Rroxscaffold_116G00451720 Rroxscaffold_117G00451760 Rroxscaffold_118G00451790 Rroxscaffold_118G00451800 Rroxscaffold_120G00451830 Rroxscaffold_122G00451890 Rroxscaffold_122G00451900 Rroxscaffold_123G00451930 Rroxscaffold_125G00451980 Rroxscaffold_126G00452010 Rroxscaffold_127G00452020 Rroxscaffold_127G00452030 Rroxscaffold_128G00452070 Rroxscaffold_12G00450630 Rroxscaffold_138G00452200 Rroxscaffold_139G00452210 Rroxscaffold_13G00448640 Rroxscaffold_13G00448680 Rroxscaffold_13G00448690 Rroxscaffold_14G00441900 Rroxscaffold_14G00441910 Rroxscaffold_14G00441920 Rroxscaffold_14G00441940 Rroxscaffold_14G00441980 Rroxscaffold_14G00441990 Rroxscaffold_14G00442000 Rroxscaffold_14G00442020 Rroxscaffold_14G00442040 Rroxscaffold_15G00447480 Rroxscaffold_15G00447520 Rroxscaffold_15G00447530 Rroxscaffold_15G00447590 Rroxscaffold_15G00447600 Rroxscaffold_162G00450740 Rroxscaffold_162G00450760 Rroxscaffold_16G00446220 Rroxscaffold_16G00446260 Rroxscaffold_17G00435370 Rroxscaffold_17G00435380 Rroxscaffold_17G00435420 Rroxscaffold_17G00435520 Rroxscaffold_17G00435540 Rroxscaffold_17G00435580 Rroxscaffold_17G00435610 Rroxscaffold_17G00435690 Rroxscaffold_17G00435710 Rroxscaffold_17G00435720 Rroxscaffold_17G00435770 Rroxscaffold_17G00435790 Rroxscaffold_17G00435850 Rroxscaffold_17G00435880 Rroxscaffold_18G00446040 Rroxscaffold_18G00446050 Rroxscaffold_18G00446070 Rroxscaffold_18G00446100 Rroxscaffold_18G00446140 Rroxscaffold_18G00446150 Rroxscaffold_18G00446180 Rroxscaffold_18G00446190 Rroxscaffold_19G00448870 Rroxscaffold_19G00448880 Rroxscaffold_19G00448900 Rroxscaffold_19G00448910 Rroxscaffold_19G00448960 Rroxscaffold_19G00448980 Rroxscaffold_1G00000030 Rroxscaffold_1G00000040 Rroxscaffold_1G00000060 Rroxscaffold_1G00000080 Rroxscaffold_20G00445050 Rroxscaffold_20G00445090 Rroxscaffold_20G00445120 Rroxscaffold_21G00439420 Rroxscaffold_21G00439450 Rroxscaffold_21G00439480 Rroxscaffold_21G00439510 Rroxscaffold_21G00439550 Rroxscaffold_21G00439600 Rroxscaffold_21G00439650 Rroxscaffold_22G00439870 Rroxscaffold_22G00439890 Rroxscaffold_22G00439900 Rroxscaffold_22G00439930 Rroxscaffold_22G00439970 Rroxscaffold_22G00440020 Rroxscaffold_23G00451030 Rroxscaffold_23G00451060 Rroxscaffold_24G00444940 Rroxscaffold_24G00444980 Rroxscaffold_24G00445020 Rroxscaffold_25G00450150 Rroxscaffold_25G00450180 Rroxscaffold_25G00450190 Rroxscaffold_26G00447750 Rroxscaffold_26G00447800 Rroxscaffold_26G00447810 Rroxscaffold_26G00447820 Rroxscaffold_27G00446580 Rroxscaffold_28G00446880 Rroxscaffold_28G00446890 Rroxscaffold_28G00446900 Rroxscaffold_28G00446920 Rroxscaffold_29G00441580 Rroxscaffold_29G00441610 Rroxscaffold_29G00441620 Rroxscaffold_29G00441650 Rroxscaffold_29G00441660 Rroxscaffold_29G00441700 Rroxscaffold_29G00441720 Rroxscaffold_29G00441770 Rroxscaffold_29G00441790 Rroxscaffold_29G00441800 Rroxscaffold_29G00441840 Rroxscaffold_29G00441880 Rroxscaffold_30G00449470 Rroxscaffold_31G00438090 Rroxscaffold_31G00438100 Rroxscaffold_31G00438150 Rroxscaffold_31G00438160 Rroxscaffold_31G00438200 Rroxscaffold_31G00438240 Rroxscaffold_31G00438280 Rroxscaffold_32G00442770 Rroxscaffold_32G00442780 Rroxscaffold_32G00442820 Rroxscaffold_33G00439680 Rroxscaffold_33G00439750 Rroxscaffold_33G00439780 Rroxscaffold_33G00439790 Rroxscaffold_33G00439830 Rroxscaffold_34G00443130 Rroxscaffold_34G00443160 Rroxscaffold_34G00443190 Rroxscaffold_34G00443240 Rroxscaffold_34G00443260 Rroxscaffold_34G00443300 Rroxscaffold_34G00443320 Rroxscaffold_34G00443340 Rroxscaffold_34G00443350 Rroxscaffold_34G00443360 Rroxscaffold_34G00443380 Rroxscaffold_35G00441020 Rroxscaffold_35G00441030 Rroxscaffold_35G00441040 Rroxscaffold_35G00441070 Rroxscaffold_35G00441100 Rroxscaffold_35G00441130 Rroxscaffold_35G00441150 Rroxscaffold_35G00441200 Rroxscaffold_35G00441240 Rroxscaffold_35G00441250 Rroxscaffold_35G00441270 Rroxscaffold_36G00440060 Rroxscaffold_36G00440090 Rroxscaffold_36G00440130 Rroxscaffold_36G00440180 Rroxscaffold_36G00440210 Rroxscaffold_36G00440220 Rroxscaffold_36G00440230 Rroxscaffold_36G00440240 Rroxscaffold_36G00440250 Rroxscaffold_37G00445140 Rroxscaffold_37G00445200 Rroxscaffold_37G00445220 Rroxscaffold_38G00444460 Rroxscaffold_38G00444490 Rroxscaffold_38G00444510 Rroxscaffold_38G00444520 Rroxscaffold_38G00444540 Rroxscaffold_38G00444560 Rroxscaffold_38G00444580 Rroxscaffold_38G00444590 Rroxscaffold_38G00444630 Rroxscaffold_39G00448220 Rroxscaffold_40G00447660 Rroxscaffold_40G00447670 Rroxscaffold_41G00451100 Rroxscaffold_41G00451120 Rroxscaffold_42G00450440 Rroxscaffold_43G00449830 Rroxscaffold_43G00449880 Rroxscaffold_43G00449910 Rroxscaffold_43G00449950 Rroxscaffold_44G00440490 Rroxscaffold_44G00440530 Rroxscaffold_44G00440560 Rroxscaffold_44G00440570 Rroxscaffold_44G00440590 Rroxscaffold_44G00440630 Rroxscaffold_45G00438860 Rroxscaffold_45G00438910 Rroxscaffold_45G00438940 Rroxscaffold_45G00438990 Rroxscaffold_47G00443990 Rroxscaffold_47G00444010 Rroxscaffold_47G00444040 Rroxscaffold_47G00444050 Rroxscaffold_47G00444070 Rroxscaffold_47G00444080 Rroxscaffold_47G00444100 Rroxscaffold_48G00448440 Rroxscaffold_48G00448460 Rroxscaffold_48G00448480 Rroxscaffold_48G00448490 Rroxscaffold_48G00448530 Rroxscaffold_49G00438580 Rroxscaffold_49G00438610 Rroxscaffold_49G00438630 Rroxscaffold_49G00438640 Rroxscaffold_49G00438670 Rroxscaffold_49G00438690 Rroxscaffold_49G00438760 Rroxscaffold_49G00438770 Rroxscaffold_50G00444140 Rroxscaffold_50G00444150 Rroxscaffold_50G00444180 Rroxscaffold_50G00444220 Rroxscaffold_50G00444230 Rroxscaffold_50G00444270 Rroxscaffold_50G00444300 Rroxscaffold_50G00444310 Rroxscaffold_50G00444320 Rroxscaffold_50G00444340 Rroxscaffold_50G00444360 Rroxscaffold_51G00447830 Rroxscaffold_51G00447870 Rroxscaffold_51G00447890 Rroxscaffold_51G00447910 Rroxscaffold_52G00439320 Rroxscaffold_52G00439330 Rroxscaffold_52G00439340 Rroxscaffold_52G00439350 Rroxscaffold_52G00439370 Rroxscaffold_52G00439400 Rroxscaffold_53G00449660 Rroxscaffold_53G00449710 Rroxscaffold_53G00449720 Rroxscaffold_53G00449750 Rroxscaffold_53G00449760 Rroxscaffold_53G00449780 Rroxscaffold_54G00443480 Rroxscaffold_54G00443510 Rroxscaffold_54G00443520 Rroxscaffold_54G00443550 Rroxscaffold_54G00443570 Rroxscaffold_54G00443600 Rroxscaffold_54G00443640 Rroxscaffold_55G00450030 Rroxscaffold_55G00450040 Rroxscaffold_55G00450100 Rroxscaffold_57G00443080 Rroxscaffold_58G00449010 Rroxscaffold_58G00449060 Rroxscaffold_59G00442090 Rroxscaffold_59G00442140 Rroxscaffold_59G00442160 Rroxscaffold_59G00442190 Rroxscaffold_59G00442230 Rroxscaffold_60G00448300 Rroxscaffold_60G00448310 Rroxscaffold_60G00448350 Rroxscaffold_60G00448390 Rroxscaffold_61G00450000 Rroxscaffold_62G00437920 Rroxscaffold_62G00437970 Rroxscaffold_62G00437980 Rroxscaffold_62G00437990 Rroxscaffold_62G00438000 Rroxscaffold_62G00438010 Rroxscaffold_62G00438020 Rroxscaffold_64G00450660 Rroxscaffold_64G00450680 Rroxscaffold_64G00450720 Rroxscaffold_65G00445290 Rroxscaffold_65G00445310 Rroxscaffold_65G00445410 Rroxscaffold_66G00437580 Rroxscaffold_66G00437590 Rroxscaffold_66G00437810 Rroxscaffold_66G00437880 Rroxscaffold_67G00448120 Rroxscaffold_67G00448160 Rroxscaffold_68G00446970 Rroxscaffold_68G00447020 Rroxscaffold_68G00447050 Rroxscaffold_68G00447060 Rroxscaffold_69G00446660 Rroxscaffold_69G00446690 Rroxscaffold_69G00446700 Rroxscaffold_69G00446720 Rroxscaffold_69G00446750 Rroxscaffold_69G00446800 Rroxscaffold_6G00387780 Rroxscaffold_6G00387810 Rroxscaffold_70G00446280 Rroxscaffold_70G00446320 Rroxscaffold_70G00446360 Rroxscaffold_70G00446370 Rroxscaffold_70G00446400 Rroxscaffold_71G00445610 Rroxscaffold_71G00445620 Rroxscaffold_71G00445640 Rroxscaffold_71G00445660 Rroxscaffold_71G00445670 Rroxscaffold_71G00445700 Rroxscaffold_71G00445760 Rroxscaffold_71G00445780 Rroxscaffold_72G00449360 Rroxscaffold_72G00449390 Rroxscaffold_72G00449420 Rroxscaffold_72G00449440 Rroxscaffold_73G00439010 Rroxscaffold_73G00439020 Rroxscaffold_73G00439040 Rroxscaffold_73G00439050 Rroxscaffold_73G00439070 Rroxscaffold_73G00439080 Rroxscaffold_73G00439100 Rroxscaffold_73G00439130 Rroxscaffold_73G00439150 Rroxscaffold_73G00439170 Rroxscaffold_73G00439200 Rroxscaffold_73G00439220 Rroxscaffold_73G00439230 Rroxscaffold_73G00439240 Rroxscaffold_73G00439260 Rroxscaffold_73G00439280 Rroxscaffold_74G00442900 Rroxscaffold_74G00442910 Rroxscaffold_74G00442930 Rroxscaffold_75G00447270 Rroxscaffold_75G00447320 Rroxscaffold_75G00447360 Rroxscaffold_75G00447400 Rroxscaffold_76G00448550 Rroxscaffold_77G00449160 Rroxscaffold_78G00449590 Rroxscaffold_78G00449600 Rroxscaffold_78G00449620 Rroxscaffold_78G00449650 Rroxscaffold_79G00450220 Rroxscaffold_79G00450230 Rroxscaffold_79G00450240 Rroxscaffold_80G00450260 Rroxscaffold_80G00450270 Rroxscaffold_80G00450340 Rroxscaffold_81G00450500 Rroxscaffold_81G00450510 Rroxscaffold_83G00450970 Rroxscaffold_83G00450990 Rroxscaffold_85G00451220 Rroxscaffold_85G00451240 Rroxscaffold_86G00451280 Rroxscaffold_87G00451310 Rroxscaffold_88G00451400 Rroxscaffold_89G00451420 Rroxscaffold_90G00448780 Rroxscaffold_90G00448790 Rroxscaffold_90G00448840 Rroxscaffold_91G00442970 Rroxscaffold_91G00443010 Rroxscaffold_92G00446420 Rroxscaffold_92G00446450 Rroxscaffold_92G00446470 Rroxscaffold_92G00446480 Rroxscaffold_92G00446510 Rroxscaffold_93G00440840 Rroxscaffold_93G00440870 Rroxscaffold_93G00440900 Rroxscaffold_93G00440940 Rroxscaffold_93G00440960 Rroxscaffold_93G00440980 Rroxscaffold_93G00441000 Rroxscaffold_94G00445840 Rroxscaffold_94G00445860 Rroxscaffold_94G00445890 Rroxscaffold_94G00445930 Rroxscaffold_94G00445980 Rroxscaffold_94G00446010 Rroxscaffold_95G00449210 Rroxscaffold_95G00449250 Rroxscaffold_95G00449280 Rroxscaffold_96G00449100 Rroxscaffold_97G00442290 Rroxscaffold_97G00442300 Rroxscaffold_97G00442310 Rroxscaffold_97G00442320 Rroxscaffold_97G00442380 Rroxscaffold_97G00442410 Rroxscaffold_98G00451360 Rroxscaffold_98G00451370 Rroxscaffold_99G00451200
rosa_samantha Rh6CG279700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 474
AccB1I GGYRCC 1 cut(s) 825
AccBSI CCGCTC 2 cut(s) 100, 303
AccII CGCG 7 cut(s) 115, 141, 195, 197, 530, 586, 736
AclWI GGATC 2 cut(s) 397, 410
AcoI YGGCCR 4 cut(s) 95, 179, 513, 568
AcsI RAATTY 1 cut(s) 784
AcuI CTGAAG 1 cut(s) 185
AcyI GRCGYC 2 cut(s) 519, 826
AfaI GTAC 2 cut(s) 553, 740
AgeI ACCGGT 1 cut(s) 790
AgsI TTSAA 5 cut(s) 47, 61, 487, 689, 712
AjiI CACGTC 1 cut(s) 254
AjnI CCWGG 2 cut(s) 119, 161
AluBI AGCT 2 cut(s) 417, 507
AluI AGCT 2 cut(s) 417, 507
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 1 cut(s) 761
AlwI GGATC 2 cut(s) 397, 410
Ama87I CYCGRG 3 cut(s) 106, 559, 600
ApaI GGGCCC 1 cut(s) 851
ApeKI GCWGC 1 cut(s) 264
ApoI RAATTY 1 cut(s) 784
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AsiGI ACCGGT 1 cut(s) 790
AsuC2I CCSGG 9 cut(s) 107, 108, 183, 523, 560, 561, 645, 797, 851
AsuHPI GGTGA 2 cut(s) 308, 806
AvaI CYCGRG 3 cut(s) 106, 559, 600
AvaII GGWCC 6 cut(s) 329, 476, 591, 615, 793, 853
BaeGI GKGCMC 2 cut(s) 802, 851
BaeI ACNNNNGTAYC 2 cut(s) 242, 275
BamHI GGATCC 1 cut(s) 402
BanI GGYRCC 1 cut(s) 825
BanII GRGCYC 4 cut(s) 113, 509, 812, 851
BauI CACGAG 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 1 cut(s) 251
BccI CCATC 3 cut(s) 216, 327, 672
BceAI ACGGC 3 cut(s) 110, 194, 570
BcgI CGANNNNNNTGC 4 cut(s) 230, 264, 323, 357
BciT130I CCWGG 2 cut(s) 121, 163
BcnI CCSGG 9 cut(s) 107, 108, 183, 523, 560, 561, 645, 797, 851
BcoDI GTCTC 1 cut(s) 761
BfaI CTAG 1 cut(s) 224
BfoI RGCGCY 3 cut(s) 169, 208, 829
BglI GCCNNNNNGGC 2 cut(s) 178, 554
BglII AGATCT 1 cut(s) 82
BisI GCNGC 8 cut(s) 98, 130, 139, 265, 571, 574, 577, 859
BlsI GCNGC 8 cut(s) 99, 131, 140, 266, 572, 575, 578, 860
Bme18I GGWCC 6 cut(s) 329, 476, 591, 615, 793, 853
BmeT110I CYCGRG 3 cut(s) 106, 559, 600
BmgBI CACGTC 1 cut(s) 254
BmiI GGNNCC 8 cut(s) 25, 160, 330, 331, 404, 558, 827, 849
BmsI GCATC 1 cut(s) 660
Bpu10I CCTNAGC 1 cut(s) 304
BpuMI CCSGG 9 cut(s) 107, 108, 183, 523, 560, 561, 645, 797, 851
BsaHI GRCGYC 2 cut(s) 519, 826
BsaI GGTCTC 1 cut(s) 761
BsaJI CCNNGG 7 cut(s) 106, 119, 472, 558, 559, 644, 775
BsaWI WCCGGW 1 cut(s) 790
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bse118I RCCGGY 3 cut(s) 548, 617, 790
Bse1I ACTGG 2 cut(s) 410, 836
BseBI CCWGG 2 cut(s) 121, 163
BseDI CCNNGG 7 cut(s) 106, 119, 472, 558, 559, 644, 775
BseGI GGATG 3 cut(s) 208, 636, 664
BseMII CTCAG 2 cut(s) 318, 376
BseNI ACTGG 2 cut(s) 410, 836
BsePI GCGCGC 2 cut(s) 195, 528
BseSI GKGCMC 2 cut(s) 802, 851
BseX3I CGGCCG 2 cut(s) 95, 179
BseXI GCAGC 1 cut(s) 251
BsgI GTGCAG 1 cut(s) 71
Bsh1236I CGCG 7 cut(s) 115, 141, 195, 197, 530, 586, 736
Bsh1285I CGRYCG 7 cut(s) 98, 136, 182, 381, 610, 629, 644
BshNI GGYRCC 1 cut(s) 825
BshTI ACCGGT 1 cut(s) 790
BsiEI CGRYCG 7 cut(s) 98, 136, 182, 381, 610, 629, 644
BsiHKAI GWGCWC 1 cut(s) 509
BsiHKCI CYCGRG 3 cut(s) 106, 559, 600
BslFI GGGAC 1 cut(s) 315
BsmAI GTCTC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 315
Bso31I GGTCTC 1 cut(s) 761
BsoBI CYCGRG 3 cut(s) 106, 559, 600
Bsp120I GGGCCC 1 cut(s) 847
Bsp1286I GDGCHC 5 cut(s) 113, 509, 802, 812, 851
Bsp143I GATC 5 cut(s) 82, 368, 402, 439, 607
BspCNI CTCAG 2 cut(s) 317, 375
BspFNI CGCG 7 cut(s) 115, 141, 195, 197, 530, 586, 736
BspLI GGNNCC 8 cut(s) 25, 160, 330, 331, 404, 558, 827, 849
BspPI GGATC 2 cut(s) 397, 410
BspT107I GGYRCC 1 cut(s) 825
BspTNI GGTCTC 1 cut(s) 761
BsrBI CCGCTC 2 cut(s) 100, 303
BsrFI RCCGGY 3 cut(s) 548, 617, 790
BsrI ACTGG 2 cut(s) 410, 836
BssAI RCCGGY 3 cut(s) 548, 617, 790
BssECI CCNNGG 7 cut(s) 106, 119, 472, 558, 559, 644, 775
BssHII GCGCGC 2 cut(s) 195, 528
BssMI GATC 5 cut(s) 82, 368, 402, 439, 607
BssNI GRCGYC 2 cut(s) 519, 826
BssSI CACGAG 1 cut(s) 395
BssT1I CCWWGG 2 cut(s) 472, 775
Bst2BI CACGAG 1 cut(s) 395
Bst2UI CCWGG 2 cut(s) 121, 163
Bst4CI ACNGT 1 cut(s) 630
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 2 cut(s) 519, 826
BstDEI CTNAG 2 cut(s) 304, 362
BstF5I GGATG 3 cut(s) 208, 636, 664
BstFNI CGCG 7 cut(s) 115, 141, 195, 197, 530, 586, 736
BstH2I RGCGCY 3 cut(s) 169, 208, 829
BstKTI GATC 5 cut(s) 85, 371, 405, 442, 610
BstMAI GTCTC 1 cut(s) 761
BstMBI GATC 5 cut(s) 82, 368, 402, 439, 607
BstMCI CGRYCG 7 cut(s) 98, 136, 182, 381, 610, 629, 644
BstNI CCWGG 2 cut(s) 121, 163
BstNSI RCATGY 2 cut(s) 344, 464
BstSLI GKGCMC 2 cut(s) 802, 851
BstUI CGCG 7 cut(s) 115, 141, 195, 197, 530, 586, 736
BstV1I GCAGC 1 cut(s) 251
BstX2I RGATCY 2 cut(s) 82, 402
BstYI RGATCY 2 cut(s) 82, 402
BstZI CGGCCG 2 cut(s) 95, 179
BtgZI GCGATG 1 cut(s) 637
BtrI CACGTC 1 cut(s) 254
BtsCI GGATG 3 cut(s) 208, 636, 664
BtsIMutI CAGTG 1 cut(s) 843
Cfr10I RCCGGY 3 cut(s) 548, 617, 790
Cfr9I CCCGGG 2 cut(s) 106, 559
CpoI CGGWCCG 3 cut(s) 615, 793, 853
CseI GACGC 2 cut(s) 527, 575
Csp6I GTAC 2 cut(s) 552, 739
CspAI ACCGGT 1 cut(s) 790
CspCI CAANNNNNGTGG 2 cut(s) 378, 413
CspI CGGWCCG 3 cut(s) 615, 793, 853
CviAII CATG 3 cut(s) 20, 341, 461
CviQI GTAC 2 cut(s) 552, 739
DdeI CTNAG 2 cut(s) 304, 362
DinI GGCGCC 1 cut(s) 827
DpnI GATC 5 cut(s) 84, 370, 404, 441, 609
DpnII GATC 5 cut(s) 82, 368, 402, 439, 607
DrdI GACNNNNNNGTC 1 cut(s) 474
DseDI GACNNNNNNGTC 1 cut(s) 474
EaeI YGGCCR 4 cut(s) 95, 179, 513, 568
EagI CGGCCG 2 cut(s) 95, 179
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 3 cut(s) 92, 628, 845
Ecl136II GAGCTC 1 cut(s) 507
EclXI CGGCCG 2 cut(s) 95, 179
Eco130I CCWWGG 2 cut(s) 472, 775
Eco24I GRGCYC 4 cut(s) 113, 509, 812, 851
Eco31I GGTCTC 1 cut(s) 761
Eco47I GGWCC 6 cut(s) 329, 476, 591, 615, 793, 853
Eco52I CGGCCG 2 cut(s) 95, 179
Eco53kI GAGCTC 1 cut(s) 507
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 3 cut(s) 106, 559, 600
EcoICRI GAGCTC 1 cut(s) 507
EcoO109I RGGNCCY 2 cut(s) 159, 329
EcoRII CCWGG 2 cut(s) 119, 161
EcoT14I CCWWGG 2 cut(s) 472, 775
EcoT38I GRGCYC 4 cut(s) 113, 509, 812, 851
EgeI GGCGCC 1 cut(s) 827
EheI GGCGCC 1 cut(s) 827
ErhI CCWWGG 2 cut(s) 472, 775
FaeI CATG 3 cut(s) 23, 344, 464
FaiI YATR 4 cut(s) 21, 311, 342, 462
FaqI GGGAC 1 cut(s) 315
FatI CATG 3 cut(s) 19, 340, 460
FauI CCCGC 3 cut(s) 260, 308, 414
Fnu4HI GCNGC 8 cut(s) 98, 130, 139, 265, 571, 574, 577, 859
FokI GGATG 3 cut(s) 195, 623, 651
FriOI GRGCYC 4 cut(s) 113, 509, 812, 851
Fsp4HI GCNGC 8 cut(s) 98, 130, 139, 265, 571, 574, 577, 859
FspBI CTAG 1 cut(s) 224
GluI GCNGC 8 cut(s) 98, 130, 139, 265, 571, 574, 577, 859
HaeII RGCGCY 3 cut(s) 169, 208, 829
HgaI GACGC 2 cut(s) 527, 575
Hin1I GRCGYC 2 cut(s) 519, 826
Hin1II CATG 3 cut(s) 23, 344, 464
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 4 cut(s) 230, 451, 468, 701
HphI GGTGA 2 cut(s) 308, 806
Hpy166II GTNNAC 5 cut(s) 17, 316, 450, 594, 841
Hpy188I TCNGA 3 cut(s) 365, 467, 641
Hpy188III TCNNGA 3 cut(s) 332, 487, 678
Hpy8I GTNNAC 5 cut(s) 17, 316, 450, 594, 841
Hpy99I CGWCG 5 cut(s) 96, 180, 521, 591, 807
HpyAV CCTTC 3 cut(s) 53, 368, 842
HpyCH4III ACNGT 1 cut(s) 630
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 4 cut(s) 6, 88, 251, 389
HpyF3I CTNAG 2 cut(s) 304, 362
HpySE526I ACGT 1 cut(s) 253
Hsp92I GRCGYC 2 cut(s) 519, 826
Hsp92II CATG 3 cut(s) 23, 344, 464
KasI GGCGCC 1 cut(s) 825
KflI GGGWCCC 1 cut(s) 329
Kzo9I GATC 5 cut(s) 82, 368, 402, 439, 607
LmnI GCTCC 3 cut(s) 105, 504, 525
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 1 cut(s) 660
MaeI CTAG 1 cut(s) 224
MaeII ACGT 1 cut(s) 253
MalI GATC 5 cut(s) 84, 370, 404, 441, 609
MbiI CCGCTC 2 cut(s) 100, 303
MboI GATC 5 cut(s) 82, 368, 402, 439, 607
MboII GAAGA 2 cut(s) 28, 378
MflI RGATCY 2 cut(s) 82, 402
MhlI GDGCHC 5 cut(s) 113, 509, 802, 812, 851
MluCI AATT 2 cut(s) 683, 784
Mly113I GGCGCC 1 cut(s) 826
MlyI GAGTC 3 cut(s) 445, 462, 695
MmeI TCCRAC 1 cut(s) 664
MnlI CCTC 8 cut(s) 44, 155, 282, 297, 404, 685, 742, 828
MspA1I CMGCKG 1 cut(s) 861
MvaI CCWGG 2 cut(s) 121, 163
MvnI CGCG 7 cut(s) 115, 141, 195, 197, 530, 586, 736
NarI GGCGCC 1 cut(s) 826
NciI CCSGG 9 cut(s) 107, 108, 183, 523, 560, 561, 645, 797, 851
NdeII GATC 5 cut(s) 82, 368, 402, 439, 607
NlaIII CATG 3 cut(s) 23, 344, 464
NlaIV GGNNCC 8 cut(s) 25, 160, 330, 331, 404, 558, 827, 849
NspI RCATGY 2 cut(s) 344, 464
PaeI GCATGC 1 cut(s) 344
PauI GCGCGC 2 cut(s) 195, 528
PcsI WCGNNNNNNNCGW 1 cut(s) 250
PfeI GAWTC 1 cut(s) 230
PinAI ACCGGT 1 cut(s) 790
PkrI GCNGC 8 cut(s) 99, 131, 140, 266, 572, 575, 578, 860
Ple19I CGATCG 1 cut(s) 610
PleI GAGTC 3 cut(s) 445, 462, 695
PluTI GGCGCC 1 cut(s) 829
PpsI GAGTC 3 cut(s) 445, 462, 695
PpuMI RGGWCCY 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 509
Psp5II RGGWCCY 1 cut(s) 329
Psp6I CCWGG 2 cut(s) 119, 161
PspGI CCWGG 2 cut(s) 119, 161
PspN4I GGNNCC 8 cut(s) 25, 160, 330, 331, 404, 558, 827, 849
PspOMI GGGCCC 1 cut(s) 847
PspPPI RGGWCCY 1 cut(s) 329
PsuI RGATCY 2 cut(s) 82, 402
PteI GCGCGC 2 cut(s) 195, 528
PvuI CGATCG 1 cut(s) 610
RsaI GTAC 2 cut(s) 553, 740
RsaNI GTAC 2 cut(s) 552, 739
Rsr2I CGGWCCG 3 cut(s) 615, 793, 853
RsrII CGGWCCG 3 cut(s) 615, 793, 853
SacI GAGCTC 1 cut(s) 509
SatI GCNGC 8 cut(s) 98, 130, 139, 265, 571, 574, 577, 859
Sau3AI GATC 5 cut(s) 82, 368, 402, 439, 607
SchI GAGTC 3 cut(s) 445, 462, 695
SduI GDGCHC 5 cut(s) 113, 509, 802, 812, 851
SetI ASST 8 cut(s) 29, 125, 193, 256, 377, 419, 509, 834
SfaNI GCATC 1 cut(s) 660
SfoI GGCGCC 1 cut(s) 827
SinI GGWCC 6 cut(s) 329, 476, 591, 615, 793, 853
SmaI CCCGGG 2 cut(s) 108, 561
SphI GCATGC 1 cut(s) 344
SrfI GCCCGGGC 1 cut(s) 108
Sse9I AATT 2 cut(s) 683, 784
SspDI GGCGCC 1 cut(s) 825
SspI AATATT 1 cut(s) 64
SspMI CTAG 1 cut(s) 224
SstI GAGCTC 1 cut(s) 509
StyI CCWWGG 2 cut(s) 472, 775
TaaI ACNGT 1 cut(s) 630
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 352, 625
TaqII GACCGA 3 cut(s) 367, 579, 743
TasI AATT 2 cut(s) 683, 784
TauI GCSGC 7 cut(s) 100, 132, 141, 573, 576, 579, 861
TfiI GAWTC 1 cut(s) 230
TscAI CASTG 1 cut(s) 843
TseI GCWGC 1 cut(s) 264
TspDTI ATGAA 1 cut(s) 712
TspGWI ACGGA 1 cut(s) 468
TspMI CCCGGG 2 cut(s) 106, 559
TspRI CASTG 1 cut(s) 843
VpaK11BI GGWCC 6 cut(s) 329, 476, 591, 615, 793, 853
XapI RAATTY 1 cut(s) 784
XceI RCATGY 2 cut(s) 344, 464
XmaI CCCGGG 2 cut(s) 106, 559
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.