Rroxscaffold_69G00446690

mitotic sister chromatid biorientation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000069
OG0000037
Physical Location & Seq
Reverse (-)
6966 .. 13513
6548 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_69G00446690.1

Sequence Viewer

Length: 759 bp
ATGCTCACACTCGAACCCTTCTCGAAGATCAAGGTCGGTCGGCGGTGCACCCGCAAAGGGATCCCGCACATTAGCTTCCTTGCGCCTTACGGGTTTAATCACCCGTTGACTCGCACACATGTCAGACTCCTTGGTCCGTGTTTCAAGACGGGCCGAATGGGGAGCCCGCAGGCCGTTACCAGGAGCACGCAGATGCCGAAGCACGCCGAGACGGCGCATGCTGCCTACCATGATCGCGTCGACGACGTCTCCACGGGCATATCAACAGCCCGGGCTTTGGCCGCCGCCGCAATCCGTAACGGTCCACGCCCCGAGTCGAGTGGCGGACCGGCTTGTGACCGTTCCACATCCGACCGAGGCGCATCGCCGGCCCCCATCCGCTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGTGGTGCGACAGGGTCCGGGCACAACGGGGCTCTCACCCTCTATGGCGCCCCCTTCCAGGGGACTTGTTCCCGGTCCGCCTCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCTTACGACGCCAGATTCTCAAGCTGGGCTATTCCCGGTTCGCTCGCCGTTACTAAGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

27.18

Weight (kDa)

11.25

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000037)

Species Orthologous Gene IDs
pyrus_communis pycom12555g00040 pycom12555g00090 pycom2132g00020 pycom2247g00020 pycom2303g00010 pycom2303g00070 pycom2303g00140 pycom2575g00040 pycom520g01330 pycom553g00030 pycom961g00020
rosa_laevigata RLG00000005153 RLG00000005811 RLG00000030826 RLG00000030829
rosa_multiflora Rmu_sc0001696.1_g000004
rosa_roxburghii Rroxscaffold_100G00451140 Rroxscaffold_100G00451150 Rroxscaffold_100G00451160 Rroxscaffold_102G00450910 Rroxscaffold_102G00450930 Rroxscaffold_103G00450540 Rroxscaffold_103G00450580 Rroxscaffold_103G00450590 Rroxscaffold_104G00444650 Rroxscaffold_104G00444700 Rroxscaffold_104G00444730 Rroxscaffold_104G00444770 Rroxscaffold_104G00444780 Rroxscaffold_104G00444820 Rroxscaffold_104G00444850 Rroxscaffold_104G00444890 Rroxscaffold_105G00447110 Rroxscaffold_105G00447150 Rroxscaffold_105G00447200 Rroxscaffold_105G00447210 Rroxscaffold_105G00447240 Rroxscaffold_105G00447250 Rroxscaffold_106G00451440 Rroxscaffold_106G00451450 Rroxscaffold_107G00442420 Rroxscaffold_107G00442430 Rroxscaffold_107G00442460 Rroxscaffold_107G00442490 Rroxscaffold_107G00442500 Rroxscaffold_107G00442530 Rroxscaffold_107G00442580 Rroxscaffold_107G00442610 Rroxscaffold_107G00442670 Rroxscaffold_108G00451460 Rroxscaffold_108G00451470 Rroxscaffold_110G00451500 Rroxscaffold_110G00451520 Rroxscaffold_111G00451570 Rroxscaffold_112G00451600 Rroxscaffold_113G00451610 Rroxscaffold_113G00451640 Rroxscaffold_114G00451660 Rroxscaffold_114G00451670 Rroxscaffold_115G00451700 Rroxscaffold_116G00451720 Rroxscaffold_117G00451760 Rroxscaffold_118G00451790 Rroxscaffold_118G00451800 Rroxscaffold_120G00451830 Rroxscaffold_122G00451890 Rroxscaffold_122G00451900 Rroxscaffold_123G00451930 Rroxscaffold_125G00451980 Rroxscaffold_126G00452010 Rroxscaffold_127G00452020 Rroxscaffold_127G00452030 Rroxscaffold_128G00452070 Rroxscaffold_12G00450630 Rroxscaffold_138G00452200 Rroxscaffold_139G00452210 Rroxscaffold_13G00448640 Rroxscaffold_13G00448680 Rroxscaffold_13G00448690 Rroxscaffold_14G00441900 Rroxscaffold_14G00441910 Rroxscaffold_14G00441920 Rroxscaffold_14G00441940 Rroxscaffold_14G00441980 Rroxscaffold_14G00441990 Rroxscaffold_14G00442000 Rroxscaffold_14G00442020 Rroxscaffold_14G00442040 Rroxscaffold_15G00447480 Rroxscaffold_15G00447520 Rroxscaffold_15G00447530 Rroxscaffold_15G00447590 Rroxscaffold_15G00447600 Rroxscaffold_162G00450740 Rroxscaffold_162G00450760 Rroxscaffold_16G00446220 Rroxscaffold_16G00446260 Rroxscaffold_17G00435370 Rroxscaffold_17G00435380 Rroxscaffold_17G00435420 Rroxscaffold_17G00435520 Rroxscaffold_17G00435540 Rroxscaffold_17G00435580 Rroxscaffold_17G00435610 Rroxscaffold_17G00435690 Rroxscaffold_17G00435710 Rroxscaffold_17G00435720 Rroxscaffold_17G00435770 Rroxscaffold_17G00435790 Rroxscaffold_17G00435850 Rroxscaffold_17G00435880 Rroxscaffold_18G00446040 Rroxscaffold_18G00446050 Rroxscaffold_18G00446070 Rroxscaffold_18G00446100 Rroxscaffold_18G00446140 Rroxscaffold_18G00446150 Rroxscaffold_18G00446180 Rroxscaffold_18G00446190 Rroxscaffold_19G00448870 Rroxscaffold_19G00448880 Rroxscaffold_19G00448900 Rroxscaffold_19G00448910 Rroxscaffold_19G00448960 Rroxscaffold_19G00448980 Rroxscaffold_1G00000030 Rroxscaffold_1G00000040 Rroxscaffold_1G00000060 Rroxscaffold_1G00000080 Rroxscaffold_20G00445050 Rroxscaffold_20G00445090 Rroxscaffold_20G00445120 Rroxscaffold_21G00439420 Rroxscaffold_21G00439450 Rroxscaffold_21G00439480 Rroxscaffold_21G00439510 Rroxscaffold_21G00439550 Rroxscaffold_21G00439600 Rroxscaffold_21G00439650 Rroxscaffold_22G00439870 Rroxscaffold_22G00439890 Rroxscaffold_22G00439900 Rroxscaffold_22G00439930 Rroxscaffold_22G00439970 Rroxscaffold_22G00440020 Rroxscaffold_23G00451030 Rroxscaffold_23G00451060 Rroxscaffold_24G00444940 Rroxscaffold_24G00444980 Rroxscaffold_24G00445020 Rroxscaffold_25G00450150 Rroxscaffold_25G00450180 Rroxscaffold_25G00450190 Rroxscaffold_26G00447750 Rroxscaffold_26G00447800 Rroxscaffold_26G00447810 Rroxscaffold_26G00447820 Rroxscaffold_27G00446580 Rroxscaffold_28G00446880 Rroxscaffold_28G00446890 Rroxscaffold_28G00446900 Rroxscaffold_28G00446920 Rroxscaffold_29G00441580 Rroxscaffold_29G00441610 Rroxscaffold_29G00441620 Rroxscaffold_29G00441650 Rroxscaffold_29G00441660 Rroxscaffold_29G00441700 Rroxscaffold_29G00441720 Rroxscaffold_29G00441770 Rroxscaffold_29G00441790 Rroxscaffold_29G00441800 Rroxscaffold_29G00441840 Rroxscaffold_29G00441880 Rroxscaffold_30G00449470 Rroxscaffold_31G00438090 Rroxscaffold_31G00438100 Rroxscaffold_31G00438150 Rroxscaffold_31G00438160 Rroxscaffold_31G00438200 Rroxscaffold_31G00438240 Rroxscaffold_31G00438280 Rroxscaffold_32G00442770 Rroxscaffold_32G00442780 Rroxscaffold_32G00442820 Rroxscaffold_33G00439680 Rroxscaffold_33G00439750 Rroxscaffold_33G00439780 Rroxscaffold_33G00439790 Rroxscaffold_33G00439830 Rroxscaffold_34G00443130 Rroxscaffold_34G00443160 Rroxscaffold_34G00443190 Rroxscaffold_34G00443240 Rroxscaffold_34G00443260 Rroxscaffold_34G00443300 Rroxscaffold_34G00443320 Rroxscaffold_34G00443340 Rroxscaffold_34G00443350 Rroxscaffold_34G00443360 Rroxscaffold_34G00443380 Rroxscaffold_35G00441020 Rroxscaffold_35G00441030 Rroxscaffold_35G00441040 Rroxscaffold_35G00441070 Rroxscaffold_35G00441100 Rroxscaffold_35G00441130 Rroxscaffold_35G00441150 Rroxscaffold_35G00441200 Rroxscaffold_35G00441240 Rroxscaffold_35G00441250 Rroxscaffold_35G00441270 Rroxscaffold_36G00440060 Rroxscaffold_36G00440090 Rroxscaffold_36G00440130 Rroxscaffold_36G00440180 Rroxscaffold_36G00440210 Rroxscaffold_36G00440220 Rroxscaffold_36G00440230 Rroxscaffold_36G00440240 Rroxscaffold_36G00440250 Rroxscaffold_37G00445140 Rroxscaffold_37G00445200 Rroxscaffold_37G00445220 Rroxscaffold_38G00444460 Rroxscaffold_38G00444490 Rroxscaffold_38G00444510 Rroxscaffold_38G00444520 Rroxscaffold_38G00444540 Rroxscaffold_38G00444560 Rroxscaffold_38G00444580 Rroxscaffold_38G00444590 Rroxscaffold_38G00444630 Rroxscaffold_39G00448220 Rroxscaffold_40G00447660 Rroxscaffold_40G00447670 Rroxscaffold_41G00451100 Rroxscaffold_41G00451120 Rroxscaffold_42G00450440 Rroxscaffold_43G00449830 Rroxscaffold_43G00449880 Rroxscaffold_43G00449910 Rroxscaffold_43G00449950 Rroxscaffold_44G00440490 Rroxscaffold_44G00440530 Rroxscaffold_44G00440560 Rroxscaffold_44G00440570 Rroxscaffold_44G00440590 Rroxscaffold_44G00440630 Rroxscaffold_45G00438860 Rroxscaffold_45G00438910 Rroxscaffold_45G00438940 Rroxscaffold_45G00438990 Rroxscaffold_47G00443990 Rroxscaffold_47G00444010 Rroxscaffold_47G00444040 Rroxscaffold_47G00444050 Rroxscaffold_47G00444070 Rroxscaffold_47G00444080 Rroxscaffold_47G00444100 Rroxscaffold_48G00448440 Rroxscaffold_48G00448460 Rroxscaffold_48G00448480 Rroxscaffold_48G00448490 Rroxscaffold_48G00448530 Rroxscaffold_49G00438580 Rroxscaffold_49G00438610 Rroxscaffold_49G00438630 Rroxscaffold_49G00438640 Rroxscaffold_49G00438670 Rroxscaffold_49G00438690 Rroxscaffold_49G00438760 Rroxscaffold_49G00438770 Rroxscaffold_50G00444140 Rroxscaffold_50G00444150 Rroxscaffold_50G00444180 Rroxscaffold_50G00444220 Rroxscaffold_50G00444230 Rroxscaffold_50G00444270 Rroxscaffold_50G00444300 Rroxscaffold_50G00444310 Rroxscaffold_50G00444320 Rroxscaffold_50G00444340 Rroxscaffold_50G00444360 Rroxscaffold_51G00447830 Rroxscaffold_51G00447870 Rroxscaffold_51G00447890 Rroxscaffold_51G00447910 Rroxscaffold_52G00439320 Rroxscaffold_52G00439330 Rroxscaffold_52G00439340 Rroxscaffold_52G00439350 Rroxscaffold_52G00439370 Rroxscaffold_52G00439400 Rroxscaffold_53G00449660 Rroxscaffold_53G00449710 Rroxscaffold_53G00449720 Rroxscaffold_53G00449750 Rroxscaffold_53G00449760 Rroxscaffold_53G00449780 Rroxscaffold_54G00443480 Rroxscaffold_54G00443510 Rroxscaffold_54G00443520 Rroxscaffold_54G00443550 Rroxscaffold_54G00443570 Rroxscaffold_54G00443600 Rroxscaffold_54G00443640 Rroxscaffold_55G00450030 Rroxscaffold_55G00450040 Rroxscaffold_55G00450100 Rroxscaffold_57G00443080 Rroxscaffold_58G00449010 Rroxscaffold_58G00449060 Rroxscaffold_59G00442090 Rroxscaffold_59G00442140 Rroxscaffold_59G00442160 Rroxscaffold_59G00442190 Rroxscaffold_59G00442230 Rroxscaffold_60G00448300 Rroxscaffold_60G00448310 Rroxscaffold_60G00448350 Rroxscaffold_60G00448390 Rroxscaffold_61G00450000 Rroxscaffold_62G00437920 Rroxscaffold_62G00437970 Rroxscaffold_62G00437980 Rroxscaffold_62G00437990 Rroxscaffold_62G00438000 Rroxscaffold_62G00438010 Rroxscaffold_62G00438020 Rroxscaffold_64G00450660 Rroxscaffold_64G00450680 Rroxscaffold_64G00450720 Rroxscaffold_65G00445290 Rroxscaffold_65G00445310 Rroxscaffold_65G00445410 Rroxscaffold_66G00437580 Rroxscaffold_66G00437590 Rroxscaffold_66G00437810 Rroxscaffold_66G00437880 Rroxscaffold_67G00448120 Rroxscaffold_67G00448160 Rroxscaffold_68G00446970 Rroxscaffold_68G00447020 Rroxscaffold_68G00447050 Rroxscaffold_68G00447060 Rroxscaffold_69G00446660 Rroxscaffold_69G00446690 Rroxscaffold_69G00446700 Rroxscaffold_69G00446720 Rroxscaffold_69G00446750 Rroxscaffold_69G00446800 Rroxscaffold_6G00387780 Rroxscaffold_6G00387810 Rroxscaffold_70G00446280 Rroxscaffold_70G00446320 Rroxscaffold_70G00446360 Rroxscaffold_70G00446370 Rroxscaffold_70G00446400 Rroxscaffold_71G00445610 Rroxscaffold_71G00445620 Rroxscaffold_71G00445640 Rroxscaffold_71G00445660 Rroxscaffold_71G00445670 Rroxscaffold_71G00445700 Rroxscaffold_71G00445760 Rroxscaffold_71G00445780 Rroxscaffold_72G00449360 Rroxscaffold_72G00449390 Rroxscaffold_72G00449420 Rroxscaffold_72G00449440 Rroxscaffold_73G00439010 Rroxscaffold_73G00439020 Rroxscaffold_73G00439040 Rroxscaffold_73G00439050 Rroxscaffold_73G00439070 Rroxscaffold_73G00439080 Rroxscaffold_73G00439100 Rroxscaffold_73G00439130 Rroxscaffold_73G00439150 Rroxscaffold_73G00439170 Rroxscaffold_73G00439200 Rroxscaffold_73G00439220 Rroxscaffold_73G00439230 Rroxscaffold_73G00439240 Rroxscaffold_73G00439260 Rroxscaffold_73G00439280 Rroxscaffold_74G00442900 Rroxscaffold_74G00442910 Rroxscaffold_74G00442930 Rroxscaffold_75G00447270 Rroxscaffold_75G00447320 Rroxscaffold_75G00447360 Rroxscaffold_75G00447400 Rroxscaffold_76G00448550 Rroxscaffold_77G00449160 Rroxscaffold_78G00449590 Rroxscaffold_78G00449600 Rroxscaffold_78G00449620 Rroxscaffold_78G00449650 Rroxscaffold_79G00450220 Rroxscaffold_79G00450230 Rroxscaffold_79G00450240 Rroxscaffold_80G00450260 Rroxscaffold_80G00450270 Rroxscaffold_80G00450340 Rroxscaffold_81G00450500 Rroxscaffold_81G00450510 Rroxscaffold_83G00450970 Rroxscaffold_83G00450990 Rroxscaffold_85G00451220 Rroxscaffold_85G00451240 Rroxscaffold_86G00451280 Rroxscaffold_87G00451310 Rroxscaffold_88G00451400 Rroxscaffold_89G00451420 Rroxscaffold_90G00448780 Rroxscaffold_90G00448790 Rroxscaffold_90G00448840 Rroxscaffold_91G00442970 Rroxscaffold_91G00443010 Rroxscaffold_92G00446420 Rroxscaffold_92G00446450 Rroxscaffold_92G00446470 Rroxscaffold_92G00446480 Rroxscaffold_92G00446510 Rroxscaffold_93G00440840 Rroxscaffold_93G00440870 Rroxscaffold_93G00440900 Rroxscaffold_93G00440940 Rroxscaffold_93G00440960 Rroxscaffold_93G00440980 Rroxscaffold_93G00441000 Rroxscaffold_94G00445840 Rroxscaffold_94G00445860 Rroxscaffold_94G00445890 Rroxscaffold_94G00445930 Rroxscaffold_94G00445980 Rroxscaffold_94G00446010 Rroxscaffold_95G00449210 Rroxscaffold_95G00449250 Rroxscaffold_95G00449280 Rroxscaffold_96G00449100 Rroxscaffold_97G00442290 Rroxscaffold_97G00442300 Rroxscaffold_97G00442310 Rroxscaffold_97G00442320 Rroxscaffold_97G00442380 Rroxscaffold_97G00442410 Rroxscaffold_98G00451360 Rroxscaffold_98G00451370 Rroxscaffold_99G00451200
rosa_samantha Rh6CG279700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 132
AatII GACGTC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 598
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 237, 447
AclWI GGATC 2 cut(s) 55, 68
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 495
AcyI GRCGYC 4 cut(s) 246, 599, 665, 675
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 7 cut(s) 57, 180, 277, 511, 609, 610, 611
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 3 cut(s) 145, 400, 423
AjnI CCWGG 2 cut(s) 179, 608
AluBI AGCT 2 cut(s) 75, 690
AluI AGCT 2 cut(s) 75, 690
Alw21I GWGCWC 2 cut(s) 50, 188
Alw26I GTCTC 3 cut(s) 203, 253, 472
Alw44I GTGCAC 1 cut(s) 46
AlwI GGATC 2 cut(s) 55, 68
Ama87I CYCGRG 2 cut(s) 270, 311
AoxI GGCC 4 cut(s) 151, 171, 279, 369
ApaLI GTGCAC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 221, 515
ApoI RAATTY 1 cut(s) 495
AspLEI GCGC 5 cut(s) 85, 217, 362, 549, 601
AspS9I GGNCC 7 cut(s) 134, 151, 302, 326, 370, 566, 626
AsuC2I CCSGG 5 cut(s) 271, 272, 570, 624, 702
AsuHPI GGTGA 2 cut(s) 92, 579
AvaI CYCGRG 2 cut(s) 270, 311
AvaII GGWCC 5 cut(s) 134, 302, 326, 566, 626
BaeGI GKGCMC 2 cut(s) 50, 575
BamHI GGATCC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 598
BanII GRGCYC 2 cut(s) 167, 585
BauI CACGAG 1 cut(s) 551
Bbv12I GWGCWC 2 cut(s) 50, 188
BbvI GCAGC 2 cut(s) 208, 502
BccI CCATC 1 cut(s) 383
BceAI ACGGC 3 cut(s) 158, 228, 698
BciT130I CCWGG 2 cut(s) 181, 610
BcnI CCSGG 5 cut(s) 271, 272, 570, 624, 702
BcoDI GTCTC 3 cut(s) 203, 253, 472
BfoI RGCGCY 2 cut(s) 550, 602
BglI GCCNNNNNGGC 1 cut(s) 212
BisI GCNGC 5 cut(s) 222, 282, 285, 288, 516
BlsI GCNGC 5 cut(s) 223, 283, 286, 289, 517
Bme1390I CCNGG 7 cut(s) 181, 271, 272, 570, 610, 624, 702
Bme18I GGWCC 5 cut(s) 134, 302, 326, 566, 626
BmeT110I CYCGRG 2 cut(s) 270, 311
BmgT120I GGNCC 7 cut(s) 134, 151, 302, 326, 370, 566, 626
BmiI GGNNCC 5 cut(s) 62, 164, 372, 567, 600
BmrFI CCNGG 7 cut(s) 181, 271, 272, 570, 610, 624, 702
BmsI GCATC 2 cut(s) 183, 371
BpmI CTGGAG 1 cut(s) 632
BpuEI CTTGAG 1 cut(s) 670
BpuMI CCSGG 5 cut(s) 271, 272, 570, 624, 702
BsaHI GRCGYC 4 cut(s) 246, 599, 665, 675
BsaI GGTCTC 1 cut(s) 472
BsaJI CCNNGG 6 cut(s) 130, 252, 270, 355, 486, 609
BsaXI ACNNNNNCTCC 2 cut(s) 233, 263
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 180, 277, 511, 609, 610, 611
Bse118I RCCGGY 2 cut(s) 328, 367
BseBI CCWGG 2 cut(s) 181, 610
BseDI CCNNGG 6 cut(s) 130, 252, 270, 355, 486, 609
BseGI GGATG 2 cut(s) 347, 375
BseLI CCNNNNNNNGG 7 cut(s) 57, 180, 277, 511, 609, 610, 611
BseMII CTCAG 1 cut(s) 626
BseSI GKGCMC 2 cut(s) 50, 575
BseXI GCAGC 2 cut(s) 208, 502
BseYI CCCAGC 1 cut(s) 690
Bsh1236I CGCG 2 cut(s) 237, 447
Bsh1285I CGRYCG 2 cut(s) 40, 355
BshFI GGCC 4 cut(s) 153, 173, 281, 371
BshNI GGYRCC 1 cut(s) 598
BsiEI CGRYCG 2 cut(s) 40, 355
BsiHKAI GWGCWC 2 cut(s) 50, 188
BsiHKCI CYCGRG 2 cut(s) 270, 311
BsiSI CCGG 6 cut(s) 271, 329, 368, 569, 624, 702
BslFI GGGAC 1 cut(s) 627
BslI CCNNNNNNNGG 7 cut(s) 57, 180, 277, 511, 609, 610, 611
BsmAI GTCTC 3 cut(s) 203, 253, 472
BsmBI CGTCTC 2 cut(s) 203, 253
BsmFI GGGAC 1 cut(s) 627
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 4 cut(s) 153, 173, 281, 371
Bso31I GGTCTC 1 cut(s) 472
BsoBI CYCGRG 2 cut(s) 270, 311
Bsp1286I GDGCHC 5 cut(s) 50, 167, 188, 575, 585
Bsp143I GATC 3 cut(s) 27, 60, 232
BspANI GGCC 4 cut(s) 153, 173, 281, 371
BspCNI CTCAG 1 cut(s) 627
BspFNI CGCG 2 cut(s) 237, 447
BspLI GGNNCC 5 cut(s) 62, 164, 372, 567, 600
BspPI GGATC 2 cut(s) 55, 68
BspT107I GGYRCC 1 cut(s) 598
BspTNI GGTCTC 1 cut(s) 472
BsrFI RCCGGY 2 cut(s) 328, 367
BssAI RCCGGY 2 cut(s) 328, 367
BssECI CCNNGG 6 cut(s) 130, 252, 270, 355, 486, 609
BssMI GATC 3 cut(s) 27, 60, 232
BssNI GRCGYC 4 cut(s) 246, 599, 665, 675
BssSI CACGAG 1 cut(s) 551
BssT1I CCWWGG 2 cut(s) 130, 486
Bst2BI CACGAG 1 cut(s) 551
Bst2UI CCWGG 2 cut(s) 181, 610
Bst4CI ACNGT 2 cut(s) 302, 341
BstACI GRCGYC 4 cut(s) 246, 599, 665, 675
BstC8I GCNNGC 7 cut(s) 167, 171, 188, 204, 219, 369, 711
BstDEI CTNAG 2 cut(s) 635, 720
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 2 cut(s) 347, 375
BstFNI CGCG 2 cut(s) 237, 447
BstH2I RGCGCY 2 cut(s) 550, 602
BstHHI GCGC 5 cut(s) 85, 217, 362, 549, 601
BstKTI GATC 3 cut(s) 30, 63, 235
BstMAI GTCTC 3 cut(s) 203, 253, 472
BstMBI GATC 3 cut(s) 27, 60, 232
BstMCI CGRYCG 2 cut(s) 40, 355
BstMWI GCNNNNNNNGC 6 cut(s) 212, 221, 281, 287, 368, 546
BstNI CCWGG 2 cut(s) 181, 610
BstNSI RCATGY 2 cut(s) 122, 221
BstSCI CCNGG 7 cut(s) 179, 269, 270, 568, 608, 622, 700
BstSLI GKGCMC 2 cut(s) 50, 575
BstUI CGCG 2 cut(s) 237, 447
BstV1I GCAGC 2 cut(s) 208, 502
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BsuRI GGCC 4 cut(s) 153, 173, 281, 371
BtgI CCRYGG 1 cut(s) 252
BtgZI GCGATG 1 cut(s) 348
BtsCI GGATG 2 cut(s) 347, 375
Cac8I GCNNGC 7 cut(s) 167, 171, 188, 204, 219, 369, 711
CfoI GCGC 5 cut(s) 85, 217, 362, 549, 601
Cfr10I RCCGGY 2 cut(s) 328, 367
Cfr13I GGNCC 7 cut(s) 134, 151, 302, 326, 370, 566, 626
Cfr9I CCCGGG 1 cut(s) 270
CpoI CGGWCCG 2 cut(s) 326, 626
CseI GACGC 4 cut(s) 226, 649, 673, 683
Csp6I GTAC 1 cut(s) 450
CspI CGGWCCG 2 cut(s) 326, 626
CviAII CATG 3 cut(s) 119, 218, 230
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 2 cut(s) 635, 720
DinI GGCGCC 1 cut(s) 600
DpnI GATC 3 cut(s) 29, 62, 234
DpnII GATC 3 cut(s) 27, 60, 232
DrdI GACNNNNNNGTC 1 cut(s) 132
DseDI GACNNNNNNGTC 1 cut(s) 132
EaeI YGGCCR 1 cut(s) 279
EciI GGCGGA 2 cut(s) 339, 618
Eco130I CCWWGG 2 cut(s) 130, 486
Eco24I GRGCYC 2 cut(s) 167, 585
Eco31I GGTCTC 1 cut(s) 472
Eco47I GGWCC 5 cut(s) 134, 302, 326, 566, 626
Eco88I CYCGRG 2 cut(s) 270, 311
EcoRII CCWGG 2 cut(s) 179, 608
EcoT14I CCWWGG 2 cut(s) 130, 486
EcoT38I GRGCYC 2 cut(s) 167, 585
EgeI GGCGCC 1 cut(s) 600
EheI GGCGCC 1 cut(s) 600
ErhI CCWWGG 2 cut(s) 130, 486
Esp3I CGTCTC 2 cut(s) 203, 253
FaeI CATG 3 cut(s) 122, 221, 233
FaiI YATR 5 cut(s) 120, 219, 231, 260, 596
FaqI GGGAC 1 cut(s) 627
FatI CATG 3 cut(s) 118, 217, 229
FauI CCCGC 3 cut(s) 59, 72, 174
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 5 cut(s) 222, 282, 285, 288, 516
FokI GGATG 2 cut(s) 334, 362
FriOI GRGCYC 2 cut(s) 167, 585
Fsp4HI GCNGC 5 cut(s) 222, 282, 285, 288, 516
GlaI GCGC 5 cut(s) 84, 216, 361, 548, 600
GluI GCNGC 5 cut(s) 222, 282, 285, 288, 516
GsaI CCCAGC 1 cut(s) 694
GsuI CTGGAG 1 cut(s) 632
HaeII RGCGCY 2 cut(s) 550, 602
HaeIII GGCC 4 cut(s) 153, 173, 281, 371
HapII CCGG 6 cut(s) 271, 329, 368, 569, 624, 702
HgaI GACGC 4 cut(s) 226, 649, 673, 683
HhaI GCGC 5 cut(s) 85, 217, 362, 549, 601
Hin1I GRCGYC 4 cut(s) 246, 599, 665, 675
Hin1II CATG 3 cut(s) 122, 221, 233
Hin6I GCGC 5 cut(s) 83, 215, 360, 547, 599
HinP1I GCGC 5 cut(s) 83, 215, 360, 547, 599
HincII GTYRAC 2 cut(s) 108, 241
HindII GTYRAC 2 cut(s) 108, 241
HinfI GANTC 7 cut(s) 109, 126, 314, 412, 535, 681, 726
HpaII CCGG 6 cut(s) 271, 329, 368, 569, 624, 702
HphI GGTGA 2 cut(s) 92, 579
Hpy166II GTNNAC 4 cut(s) 48, 108, 241, 305
Hpy188I TCNGA 4 cut(s) 125, 352, 636, 663
Hpy188III TCNNGA 4 cut(s) 22, 145, 389, 649
Hpy8I GTNNAC 4 cut(s) 48, 108, 241, 305
Hpy99I CGWCG 4 cut(s) 242, 245, 248, 677
HpyAV CCTTC 2 cut(s) 28, 615
HpyCH4III ACNGT 2 cut(s) 302, 341
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 2 cut(s) 48, 518
HpyF10VI GCNNNNNNNGC 6 cut(s) 212, 221, 281, 287, 368, 546
HpyF3I CTNAG 2 cut(s) 635, 720
HpySE526I ACGT 1 cut(s) 246
Hsp92I GRCGYC 4 cut(s) 246, 599, 665, 675
Hsp92II CATG 3 cut(s) 122, 221, 233
HspAI GCGC 5 cut(s) 83, 215, 360, 547, 599
KasI GGCGCC 1 cut(s) 598
KroI GCCGGC 1 cut(s) 367
KroNI GCCGGC 1 cut(s) 369
Kzo9I GATC 3 cut(s) 27, 60, 232
LmnI GCTCC 2 cut(s) 162, 183
Lsp1109I GCAGC 2 cut(s) 208, 502
LweI GCATC 2 cut(s) 183, 371
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 4 cut(s) 175, 296, 335, 715
MalI GATC 3 cut(s) 29, 62, 234
MboI GATC 3 cut(s) 27, 60, 232
MboII GAAGA 1 cut(s) 37
MflI RGATCY 1 cut(s) 60
MhlI GDGCHC 5 cut(s) 50, 167, 188, 575, 585
MluCI AATT 3 cut(s) 394, 495, 657
Mly113I GGCGCC 1 cut(s) 599
MlyI GAGTC 5 cut(s) 103, 120, 323, 406, 529
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 8 cut(s) 350, 396, 453, 560, 601, 630, 642, 757
MroNI GCCGGC 1 cut(s) 367
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 191
MspI CCGG 6 cut(s) 271, 329, 368, 569, 624, 702
MspR9I CCNGG 7 cut(s) 181, 271, 272, 570, 610, 624, 702
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 2 cut(s) 181, 610
MvnI CGCG 2 cut(s) 237, 447
MwoI GCNNNNNNNGC 6 cut(s) 212, 221, 281, 287, 368, 546
NaeI GCCGGC 1 cut(s) 369
NarI GGCGCC 1 cut(s) 599
NciI CCSGG 5 cut(s) 271, 272, 570, 624, 702
NdeII GATC 3 cut(s) 27, 60, 232
NgoMIV GCCGGC 1 cut(s) 367
NlaIII CATG 3 cut(s) 122, 221, 233
NlaIV GGNNCC 5 cut(s) 62, 164, 372, 567, 600
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 335
NspI RCATGY 2 cut(s) 122, 221
PaeI GCATGC 1 cut(s) 221
PciI ACATGT 1 cut(s) 118
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PctI GAATGC 1 cut(s) 518
PdiI GCCGGC 1 cut(s) 369
PfeI GAWTC 2 cut(s) 681, 726
PflFI GACNNNGTC 2 cut(s) 245, 564
PkrI GCNGC 5 cut(s) 223, 283, 286, 289, 517
PleI GAGTC 5 cut(s) 103, 120, 322, 406, 529
PluTI GGCGCC 1 cut(s) 602
PpsI GAGTC 5 cut(s) 103, 120, 322, 406, 529
PscI ACATGT 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 179, 608
PspFI CCCAGC 1 cut(s) 690
PspGI CCWGG 2 cut(s) 179, 608
PspN4I GGNNCC 5 cut(s) 62, 164, 372, 567, 600
PspPI GGNCC 7 cut(s) 134, 151, 302, 326, 370, 566, 626
PsuI RGATCY 1 cut(s) 60
PsyI GACNNNGTC 2 cut(s) 245, 564
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 191
Rsr2I CGGWCCG 2 cut(s) 326, 626
RsrII CGGWCCG 2 cut(s) 326, 626
SalI GTCGAC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 5 cut(s) 222, 282, 285, 288, 516
Sau3AI GATC 3 cut(s) 27, 60, 232
Sau96I GGNCC 7 cut(s) 134, 151, 302, 326, 370, 566, 626
SchI GAGTC 5 cut(s) 103, 120, 323, 406, 529
ScrFI CCNGG 7 cut(s) 181, 271, 272, 570, 610, 624, 702
SduI GDGCHC 5 cut(s) 50, 167, 188, 575, 585
SetI ASST 4 cut(s) 36, 77, 249, 692
SfaNI GCATC 2 cut(s) 183, 371
SfoI GGCGCC 1 cut(s) 600
SgrDI CGTCGACG 1 cut(s) 239
SinI GGWCC 5 cut(s) 134, 302, 326, 566, 626
SmaI CCCGGG 1 cut(s) 272
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
SphI GCATGC 1 cut(s) 221
SrfI GCCCGGGC 1 cut(s) 272
Sse9I AATT 3 cut(s) 394, 495, 657
SspDI GGCGCC 1 cut(s) 598
StyD4I CCNGG 7 cut(s) 179, 269, 270, 568, 608, 622, 700
StyI CCWWGG 2 cut(s) 130, 486
TaaI ACNGT 2 cut(s) 302, 341
TaiI ACGT 1 cut(s) 249
TaqI TCGA 4 cut(s) 12, 23, 240, 317
TaqII GACCGA 3 cut(s) 26, 369, 454
TasI AATT 3 cut(s) 394, 495, 657
TauI GCSGC 3 cut(s) 284, 287, 290
TfiI GAWTC 2 cut(s) 681, 726
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TseFI GTSAC 1 cut(s) 335
TseI GCWGC 2 cut(s) 221, 515
Tsp45I GTSAC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 423
TspGWI ACGGA 3 cut(s) 126, 284, 507
TspMI CCCGGG 1 cut(s) 270
Tth111I GACNNNGTC 2 cut(s) 245, 564
VneI GTGCAC 1 cut(s) 46
VpaK11BI GGWCC 5 cut(s) 134, 302, 326, 566, 626
XapI RAATTY 1 cut(s) 495
XceI RCATGY 2 cut(s) 122, 221
XmaI CCCGGG 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 240
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.