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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 954 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 131 |
| AatII | GACGTC | 1 cut(s) | 245 |
| Acc36I | ACCTGC | 1 cut(s) | 954 |
| AccB1I | GGYRCC | 2 cut(s) | 446, 966 |
| AccI | GTMKAC | 2 cut(s) | 123, 236 |
| AccII | CGCG | 1 cut(s) | 233 |
| AccIII | TCCGGA | 1 cut(s) | 766 |
| AclWI | GGATC | 3 cut(s) | 55, 68, 993 |
| AcoI | YGGCCR | 2 cut(s) | 149, 273 |
| AcsI | RAATTY | 1 cut(s) | 345 |
| AcyI | GRCGYC | 4 cut(s) | 242, 447, 512, 522 |
| AfaI | GTAC | 1 cut(s) | 499 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 57, 271, 341, 361, 456, 457, 458, 773, 1003 |
| AflIII | ACRYGT | 2 cut(s) | 118, 940 |
| AgsI | TTSAA | 5 cut(s) | 144, 618, 733, 794, 937 |
| AjnI | CCWGG | 1 cut(s) | 455 |
| AleI | CACNNNNGTG | 1 cut(s) | 817 |
| AluBI | AGCT | 1 cut(s) | 75 |
| AluI | AGCT | 1 cut(s) | 75 |
| Alw21I | GWGCWC | 2 cut(s) | 50, 184 |
| Alw26I | GTCTC | 4 cut(s) | 199, 249, 768, 1020 |
| Alw44I | GTGCAC | 1 cut(s) | 46 |
| AlwI | GGATC | 3 cut(s) | 55, 68, 993 |
| Ama87I | CYCGRG | 3 cut(s) | 305, 481, 831 |
| Aor13HI | TCCGGA | 1 cut(s) | 766 |
| AoxI | GGCC | 2 cut(s) | 149, 273 |
| ApaLI | GTGCAC | 1 cut(s) | 46 |
| ApeKI | GCWGC | 2 cut(s) | 167, 365 |
| ApoI | RAATTY | 1 cut(s) | 345 |
| AspA2I | CCTAGG | 1 cut(s) | 887 |
| AspLEI | GCGC | 8 cut(s) | 85, 213, 399, 449, 606, 807, 851, 903 |
| AspS9I | GGNCC | 4 cut(s) | 133, 296, 320, 415 |
| AsuC2I | CCSGG | 5 cut(s) | 266, 539, 832, 833, 1004 |
| AsuHPI | GGTGA | 3 cut(s) | 92, 427, 750 |
| AvaI | CYCGRG | 3 cut(s) | 305, 481, 831 |
| AvaII | GGWCC | 4 cut(s) | 133, 296, 320, 415 |
| AvrII | CCTAGG | 1 cut(s) | 887 |
| BaeGI | GKGCMC | 2 cut(s) | 50, 586 |
| BamHI | GGATCC | 1 cut(s) | 60 |
| BanI | GGYRCC | 2 cut(s) | 446, 966 |
| BanII | GRGCYC | 2 cut(s) | 165, 433 |
| BauI | CACGAG | 1 cut(s) | 401 |
| BbsI | GAAGAC | 1 cut(s) | 740 |
| Bbv12I | GWGCWC | 2 cut(s) | 50, 184 |
| BbvI | GCAGC | 2 cut(s) | 179, 352 |
| BccI | CCATC | 1 cut(s) | 621 |
| BceAI | ACGGC | 7 cut(s) | 155, 164, 224, 266, 544, 787, 916 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 103, 137 |
| BciT130I | CCWGG | 1 cut(s) | 457 |
| BcnI | CCSGG | 5 cut(s) | 266, 539, 832, 833, 1004 |
| BcoDI | GTCTC | 4 cut(s) | 199, 249, 768, 1020 |
| BfaI | CTAG | 3 cut(s) | 888, 959, 1030 |
| BfoI | RGCGCY | 2 cut(s) | 400, 450 |
| BfuAI | ACCTGC | 1 cut(s) | 954 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 208 |
| BisI | GCNGC | 8 cut(s) | 168, 218, 276, 279, 282, 366, 525, 909 |
| BlnI | CCTAGG | 1 cut(s) | 887 |
| BlsI | GCNGC | 8 cut(s) | 169, 219, 277, 280, 283, 367, 526, 910 |
| Bme1390I | CCNGG | 6 cut(s) | 266, 457, 539, 832, 833, 1004 |
| Bme18I | GGWCC | 4 cut(s) | 133, 296, 320, 415 |
| BmeT110I | CYCGRG | 3 cut(s) | 305, 481, 831 |
| BmgT120I | GGNCC | 4 cut(s) | 133, 296, 320, 415 |
| BmiI | GGNNCC | 4 cut(s) | 62, 162, 448, 968 |
| BmrFI | CCNGG | 6 cut(s) | 266, 457, 539, 832, 833, 1004 |
| BmsI | GCATC | 3 cut(s) | 179, 676, 1020 |
| BpiI | GAAGAC | 1 cut(s) | 740 |
| BpuEI | CTTGAG | 1 cut(s) | 517 |
| BpuMI | CCSGG | 5 cut(s) | 266, 539, 832, 833, 1004 |
| Bsa29I | ATCGAT | 1 cut(s) | 684 |
| BsaAI | YACGTR | 1 cut(s) | 581 |
| BsaHI | GRCGYC | 4 cut(s) | 242, 447, 512, 522 |
| BsaJI | CCNNGG | 8 cut(s) | 129, 248, 336, 456, 800, 831, 887, 1002 |
| BsaWI | WCCGGW | 1 cut(s) | 766 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 229, 259 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 57, 271, 341, 361, 456, 457, 458, 773, 1003 |
| Bse118I | RCCGGY | 3 cut(s) | 322, 816, 969 |
| BseAI | TCCGGA | 1 cut(s) | 766 |
| BseBI | CCWGG | 1 cut(s) | 457 |
| BseCI | ATCGAT | 1 cut(s) | 684 |
| BseDI | CCNNGG | 8 cut(s) | 129, 248, 336, 456, 800, 831, 887, 1002 |
| BseGI | GGATG | 1 cut(s) | 990 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 57, 271, 341, 361, 456, 457, 458, 773, 1003 |
| BseSI | GKGCMC | 2 cut(s) | 50, 586 |
| BseX3I | CGGCCG | 1 cut(s) | 149 |
| BseXI | GCAGC | 2 cut(s) | 179, 352 |
| Bsh1236I | CGCG | 1 cut(s) | 233 |
| Bsh1285I | CGRYCG | 5 cut(s) | 40, 152, 360, 952, 1008 |
| BshFI | GGCC | 2 cut(s) | 151, 275 |
| BshNI | GGYRCC | 2 cut(s) | 446, 966 |
| BshVI | ATCGAT | 1 cut(s) | 684 |
| BsiEI | CGRYCG | 5 cut(s) | 40, 152, 360, 952, 1008 |
| BsiHKAI | GWGCWC | 2 cut(s) | 50, 184 |
| BsiHKCI | CYCGRG | 3 cut(s) | 305, 481, 831 |
| BsiSI | CCGG | 9 cut(s) | 266, 323, 418, 538, 767, 817, 832, 970, 1004 |
| BslFI | GGGAC | 1 cut(s) | 474 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 57, 271, 341, 361, 456, 457, 458, 773, 1003 |
| BsmAI | GTCTC | 4 cut(s) | 199, 249, 768, 1020 |
| BsmBI | CGTCTC | 3 cut(s) | 199, 249, 768 |
| BsmFI | GGGAC | 1 cut(s) | 474 |
| BsmI | GAATGC | 2 cut(s) | 368, 527 |
| BsnI | GGCC | 2 cut(s) | 151, 275 |
| BsoBI | CYCGRG | 3 cut(s) | 305, 481, 831 |
| Bsp1286I | GDGCHC | 5 cut(s) | 50, 165, 184, 433, 586 |
| Bsp13I | TCCGGA | 1 cut(s) | 766 |
| Bsp143I | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| BspANI | GGCC | 2 cut(s) | 151, 275 |
| BspDI | ATCGAT | 1 cut(s) | 684 |
| BspEI | TCCGGA | 1 cut(s) | 766 |
| BspFNI | CGCG | 1 cut(s) | 233 |
| BspLI | GGNNCC | 4 cut(s) | 62, 162, 448, 968 |
| BspMI | ACCTGC | 1 cut(s) | 954 |
| BspPI | GGATC | 3 cut(s) | 55, 68, 993 |
| BspT107I | GGYRCC | 2 cut(s) | 446, 966 |
| BsrFI | RCCGGY | 3 cut(s) | 322, 816, 969 |
| BssAI | RCCGGY | 3 cut(s) | 322, 816, 969 |
| BssECI | CCNNGG | 8 cut(s) | 129, 248, 336, 456, 800, 831, 887, 1002 |
| BssMI | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| BssNI | GRCGYC | 4 cut(s) | 242, 447, 512, 522 |
| BssSI | CACGAG | 1 cut(s) | 401 |
| BssT1I | CCWWGG | 3 cut(s) | 129, 800, 887 |
| Bst2BI | CACGAG | 1 cut(s) | 401 |
| Bst2UI | CCWGG | 1 cut(s) | 457 |
| Bst4CI | ACNGT | 2 cut(s) | 296, 762 |
| BstACI | GRCGYC | 4 cut(s) | 242, 447, 512, 522 |
| BstBAI | YACGTR | 1 cut(s) | 581 |
| BstC8I | GCNNGC | 8 cut(s) | 165, 184, 200, 215, 557, 828, 853, 971 |
| BstDEI | CTNAG | 1 cut(s) | 566 |
| BstDSI | CCRYGG | 1 cut(s) | 248 |
| BstF5I | GGATG | 1 cut(s) | 990 |
| BstFNI | CGCG | 1 cut(s) | 233 |
| BstH2I | RGCGCY | 2 cut(s) | 400, 450 |
| BstHHI | GCGC | 8 cut(s) | 85, 213, 399, 449, 606, 807, 851, 903 |
| BstKTI | GATC | 7 cut(s) | 30, 63, 231, 360, 758, 845, 1001 |
| BstMAI | GTCTC | 4 cut(s) | 199, 249, 768, 1020 |
| BstMBI | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| BstMCI | CGRYCG | 5 cut(s) | 40, 152, 360, 952, 1008 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 208, 217, 275, 281, 396, 610, 780, 813 |
| BstNI | CCWGG | 1 cut(s) | 457 |
| BstNSI | RCATGY | 3 cut(s) | 122, 217, 944 |
| BstSCI | CCNGG | 6 cut(s) | 264, 455, 537, 830, 831, 1002 |
| BstSLI | GKGCMC | 2 cut(s) | 50, 586 |
| BstUI | CGCG | 1 cut(s) | 233 |
| BstV1I | GCAGC | 2 cut(s) | 179, 352 |
| BstV2I | GAAGAC | 1 cut(s) | 740 |
| BstX2I | RGATCY | 1 cut(s) | 60 |
| BstYI | RGATCY | 1 cut(s) | 60 |
| BstZI | CGGCCG | 1 cut(s) | 149 |
| Bsu15I | ATCGAT | 1 cut(s) | 684 |
| BsuRI | GGCC | 2 cut(s) | 151, 275 |
| BsuTUI | ATCGAT | 1 cut(s) | 684 |
| BtgI | CCRYGG | 1 cut(s) | 248 |
| BtsCI | GGATG | 1 cut(s) | 990 |
| BveI | ACCTGC | 1 cut(s) | 954 |
| Cac8I | GCNNGC | 8 cut(s) | 165, 184, 200, 215, 557, 828, 853, 971 |
| CfoI | GCGC | 8 cut(s) | 85, 213, 399, 449, 606, 807, 851, 903 |
| Cfr10I | RCCGGY | 3 cut(s) | 322, 816, 969 |
| Cfr13I | GGNCC | 4 cut(s) | 133, 296, 320, 415 |
| Cfr9I | CCCGGG | 1 cut(s) | 831 |
| ClaI | ATCGAT | 1 cut(s) | 684 |
| CpoI | CGGWCCG | 1 cut(s) | 320 |
| CseI | GACGC | 4 cut(s) | 222, 496, 520, 530 |
| Csp6I | GTAC | 1 cut(s) | 498 |
| CspI | CGGWCCG | 1 cut(s) | 320 |
| CviAII | CATG | 4 cut(s) | 119, 214, 226, 941 |
| CviQI | GTAC | 1 cut(s) | 498 |
| DdeI | CTNAG | 1 cut(s) | 566 |
| DinI | GGCGCC | 1 cut(s) | 448 |
| DpnI | GATC | 7 cut(s) | 29, 62, 230, 359, 757, 844, 1000 |
| DpnII | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 131 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 131 |
| EaeI | YGGCCR | 2 cut(s) | 149, 273 |
| EagI | CGGCCG | 1 cut(s) | 149 |
| EciI | GGCGGA | 5 cut(s) | 333, 464, 910, 983, 988 |
| EclXI | CGGCCG | 1 cut(s) | 149 |
| Eco130I | CCWWGG | 3 cut(s) | 129, 800, 887 |
| Eco24I | GRGCYC | 2 cut(s) | 165, 433 |
| Eco32I | GATATC | 1 cut(s) | 702 |
| Eco47I | GGWCC | 4 cut(s) | 133, 296, 320, 415 |
| Eco52I | CGGCCG | 1 cut(s) | 149 |
| Eco88I | CYCGRG | 3 cut(s) | 305, 481, 831 |
| EcoRII | CCWGG | 1 cut(s) | 455 |
| EcoRV | GATATC | 1 cut(s) | 702 |
| EcoT14I | CCWWGG | 3 cut(s) | 129, 800, 887 |
| EcoT38I | GRGCYC | 2 cut(s) | 165, 433 |
| EgeI | GGCGCC | 1 cut(s) | 448 |
| EheI | GGCGCC | 1 cut(s) | 448 |
| ErhI | CCWWGG | 3 cut(s) | 129, 800, 887 |
| Esp3I | CGTCTC | 3 cut(s) | 199, 249, 768 |
| FaeI | CATG | 4 cut(s) | 122, 217, 229, 944 |
| FaiI | YATR | 8 cut(s) | 120, 215, 227, 255, 444, 728, 942, 1020 |
| FaqI | GGGAC | 1 cut(s) | 474 |
| FatI | CATG | 4 cut(s) | 118, 213, 225, 940 |
| FauI | CCCGC | 6 cut(s) | 59, 72, 172, 757, 767, 936 |
| FblI | GTMKAC | 2 cut(s) | 123, 236 |
| Fnu4HI | GCNGC | 8 cut(s) | 168, 218, 276, 279, 282, 366, 525, 909 |
| FokI | GGATG | 1 cut(s) | 977 |
| FriOI | GRGCYC | 2 cut(s) | 165, 433 |
| Fsp4HI | GCNGC | 8 cut(s) | 168, 218, 276, 279, 282, 366, 525, 909 |
| FspBI | CTAG | 3 cut(s) | 888, 959, 1030 |
| GlaI | GCGC | 8 cut(s) | 84, 212, 398, 448, 605, 806, 850, 902 |
| GluI | GCNGC | 8 cut(s) | 168, 218, 276, 279, 282, 366, 525, 909 |
| HaeII | RGCGCY | 2 cut(s) | 400, 450 |
| HaeIII | GGCC | 2 cut(s) | 151, 275 |
| HapII | CCGG | 9 cut(s) | 266, 323, 418, 538, 767, 817, 832, 970, 1004 |
| HgaI | GACGC | 4 cut(s) | 222, 496, 520, 530 |
| HhaI | GCGC | 8 cut(s) | 85, 213, 399, 449, 606, 807, 851, 903 |
| Hin1I | GRCGYC | 4 cut(s) | 242, 447, 512, 522 |
| Hin1II | CATG | 4 cut(s) | 122, 217, 229, 944 |
| Hin6I | GCGC | 8 cut(s) | 83, 211, 397, 447, 604, 805, 849, 901 |
| HinP1I | GCGC | 8 cut(s) | 83, 211, 397, 447, 604, 805, 849, 901 |
| HincII | GTYRAC | 3 cut(s) | 108, 124, 237 |
| HindII | GTYRAC | 3 cut(s) | 108, 124, 237 |
| HinfI | GANTC | 9 cut(s) | 109, 125, 308, 385, 572, 622, 639, 715, 976 |
| HpaII | CCGG | 9 cut(s) | 266, 323, 418, 538, 767, 817, 832, 970, 1004 |
| HphI | GGTGA | 3 cut(s) | 92, 427, 750 |
| Hpy166II | GTNNAC | 6 cut(s) | 48, 108, 124, 237, 299, 633 |
| Hpy188I | TCNGA | 2 cut(s) | 340, 510 |
| Hpy188III | TCNNGA | 5 cut(s) | 22, 144, 481, 767, 1025 |
| Hpy8I | GTNNAC | 6 cut(s) | 48, 108, 124, 237, 299, 633 |
| Hpy99I | CGWCG | 5 cut(s) | 238, 241, 244, 524, 742 |
| HpyAV | CCTTC | 2 cut(s) | 28, 462 |
| HpyCH4III | ACNGT | 2 cut(s) | 296, 762 |
| HpyCH4IV | ACGT | 3 cut(s) | 242, 580, 674 |
| HpyCH4V | TGCA | 4 cut(s) | 48, 368, 645, 865 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 208, 217, 275, 281, 396, 610, 780, 813 |
| HpyF3I | CTNAG | 1 cut(s) | 566 |
| HpySE526I | ACGT | 3 cut(s) | 242, 580, 674 |
| Hsp92I | GRCGYC | 4 cut(s) | 242, 447, 512, 522 |
| Hsp92II | CATG | 4 cut(s) | 122, 217, 229, 944 |
| HspAI | GCGC | 8 cut(s) | 83, 211, 397, 447, 604, 805, 849, 901 |
| KasI | GGCGCC | 1 cut(s) | 446 |
| Kpn2I | TCCGGA | 1 cut(s) | 766 |
| KroI | GCCGGC | 1 cut(s) | 969 |
| KroNI | GCCGGC | 1 cut(s) | 971 |
| Kzo9I | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| LmnI | GCTCC | 1 cut(s) | 160 |
| Lsp1109I | GCAGC | 2 cut(s) | 179, 352 |
| LweI | GCATC | 3 cut(s) | 179, 676, 1020 |
| MaeI | CTAG | 3 cut(s) | 888, 959, 1030 |
| MaeII | ACGT | 3 cut(s) | 242, 580, 674 |
| MaeIII | GTNAC | 5 cut(s) | 172, 290, 329, 561, 986 |
| MalI | GATC | 7 cut(s) | 29, 62, 230, 359, 757, 844, 1000 |
| MboI | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| MboII | GAAGA | 3 cut(s) | 37, 670, 745 |
| MflI | RGATCY | 1 cut(s) | 60 |
| MhlI | GDGCHC | 5 cut(s) | 50, 165, 184, 433, 586 |
| MluCI | AATT | 4 cut(s) | 345, 504, 646, 808 |
| Mly113I | GGCGCC | 1 cut(s) | 447 |
| MlyI | GAGTC | 7 cut(s) | 103, 119, 317, 379, 616, 724, 985 |
| MnlI | CCTC | 9 cut(s) | 346, 410, 449, 477, 488, 596, 829, 894, 928 |
| MroI | TCCGGA | 1 cut(s) | 766 |
| MroNI | GCCGGC | 1 cut(s) | 969 |
| MseI | TTAA | 1 cut(s) | 96 |
| MslI | CAYNNNNRTG | 3 cut(s) | 187, 817, 860 |
| MspI | CCGG | 9 cut(s) | 266, 323, 418, 538, 767, 817, 832, 970, 1004 |
| MspR9I | CCNGG | 6 cut(s) | 266, 457, 539, 832, 833, 1004 |
| Mva1269I | GAATGC | 2 cut(s) | 368, 527 |
| MvaI | CCWGG | 1 cut(s) | 457 |
| MvnI | CGCG | 1 cut(s) | 233 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 208, 217, 275, 281, 396, 610, 780, 813 |
| NaeI | GCCGGC | 1 cut(s) | 971 |
| NarI | GGCGCC | 1 cut(s) | 447 |
| NciI | CCSGG | 5 cut(s) | 266, 539, 832, 833, 1004 |
| NdeII | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| NgoMIV | GCCGGC | 1 cut(s) | 969 |
| NlaIII | CATG | 4 cut(s) | 122, 217, 229, 944 |
| NlaIV | GGNNCC | 4 cut(s) | 62, 162, 448, 968 |
| NmeAIII | GCCGAG | 3 cut(s) | 228, 721, 736 |
| NmuCI | GTSAC | 1 cut(s) | 329 |
| NspI | RCATGY | 3 cut(s) | 122, 217, 944 |
| OliI | CACNNNNGTG | 1 cut(s) | 817 |
| PaeI | GCATGC | 1 cut(s) | 217 |
| PaeR7I | CTCGAG | 1 cut(s) | 481 |
| PaqCI | CACCTGC | 1 cut(s) | 954 |
| PciI | ACATGT | 2 cut(s) | 118, 940 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 190 |
| PctI | GAATGC | 2 cut(s) | 368, 527 |
| PdiI | GCCGGC | 1 cut(s) | 971 |
| PfeI | GAWTC | 2 cut(s) | 572, 639 |
| PflFI | GACNNNGTC | 1 cut(s) | 241 |
| PkrI | GCNGC | 8 cut(s) | 169, 219, 277, 280, 283, 367, 526, 910 |
| Ple19I | CGATCG | 1 cut(s) | 360 |
| PleI | GAGTC | 7 cut(s) | 103, 119, 316, 379, 616, 723, 984 |
| PluTI | GGCGCC | 1 cut(s) | 450 |
| PpsI | GAGTC | 7 cut(s) | 103, 119, 316, 379, 616, 723, 984 |
| Ppu21I | YACGTR | 1 cut(s) | 581 |
| PscI | ACATGT | 2 cut(s) | 118, 940 |
| Psp6I | CCWGG | 1 cut(s) | 455 |
| PspGI | CCWGG | 1 cut(s) | 455 |
| PspN4I | GGNNCC | 4 cut(s) | 62, 162, 448, 968 |
| PspPI | GGNCC | 4 cut(s) | 133, 296, 320, 415 |
| PsuI | RGATCY | 1 cut(s) | 60 |
| PsyI | GACNNNGTC | 1 cut(s) | 241 |
| PvuI | CGATCG | 1 cut(s) | 360 |
| RsaI | GTAC | 1 cut(s) | 499 |
| RsaNI | GTAC | 1 cut(s) | 498 |
| RseI | CAYNNNNRTG | 3 cut(s) | 187, 817, 860 |
| Rsr2I | CGGWCCG | 1 cut(s) | 320 |
| RsrII | CGGWCCG | 1 cut(s) | 320 |
| SalI | GTCGAC | 2 cut(s) | 122, 235 |
| SaqAI | TTAA | 1 cut(s) | 96 |
| SatI | GCNGC | 8 cut(s) | 168, 218, 276, 279, 282, 366, 525, 909 |
| Sau3AI | GATC | 7 cut(s) | 27, 60, 228, 357, 755, 842, 998 |
| Sau96I | GGNCC | 4 cut(s) | 133, 296, 320, 415 |
| SchI | GAGTC | 7 cut(s) | 103, 119, 317, 379, 616, 724, 985 |
| ScrFI | CCNGG | 6 cut(s) | 266, 457, 539, 832, 833, 1004 |
| SduI | GDGCHC | 5 cut(s) | 50, 165, 184, 433, 586 |
| SetI | ASST | 7 cut(s) | 36, 77, 245, 417, 583, 677, 968 |
| SfaNI | GCATC | 3 cut(s) | 179, 676, 1020 |
| SfoI | GGCGCC | 1 cut(s) | 448 |
| Sfr274I | CTCGAG | 1 cut(s) | 481 |
| SgrAI | CRCCGGYG | 1 cut(s) | 816 |
| SgrDI | CGTCGACG | 1 cut(s) | 235 |
| SinI | GGWCC | 4 cut(s) | 133, 296, 320, 415 |
| SlaI | CTCGAG | 1 cut(s) | 481 |
| SmaI | CCCGGG | 1 cut(s) | 833 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 187, 817, 860 |
| SmlI | CTYRAG | 2 cut(s) | 481, 532 |
| SmoI | CTYRAG | 2 cut(s) | 481, 532 |
| SphI | GCATGC | 1 cut(s) | 217 |
| Sse9I | AATT | 4 cut(s) | 345, 504, 646, 808 |
| SspDI | GGCGCC | 1 cut(s) | 446 |
| SspMI | CTAG | 3 cut(s) | 888, 959, 1030 |
| StyD4I | CCNGG | 6 cut(s) | 264, 455, 537, 830, 831, 1002 |
| StyI | CCWWGG | 3 cut(s) | 129, 800, 887 |
| TaaI | ACNGT | 2 cut(s) | 296, 762 |
| TaiI | ACGT | 3 cut(s) | 245, 583, 677 |
| TaqII | GACCGA | 2 cut(s) | 26, 938 |
| TasI | AATT | 4 cut(s) | 345, 504, 646, 808 |
| TauI | GCSGC | 6 cut(s) | 220, 278, 281, 284, 527, 911 |
| TfiI | GAWTC | 2 cut(s) | 572, 639 |
| Tru1I | TTAA | 1 cut(s) | 96 |
| Tru9I | TTAA | 1 cut(s) | 96 |
| TseFI | GTSAC | 1 cut(s) | 329 |
| TseI | GCWGC | 2 cut(s) | 167, 365 |
| Tsp45I | GTSAC | 1 cut(s) | 329 |
| TspDTI | ATGAA | 2 cut(s) | 670, 778 |
| TspGWI | ACGGA | 5 cut(s) | 125, 278, 357, 485, 694 |
| TspMI | CCCGGG | 1 cut(s) | 831 |
| Tth111I | GACNNNGTC | 1 cut(s) | 241 |
| VneI | GTGCAC | 1 cut(s) | 46 |
| VpaK11BI | GGWCC | 4 cut(s) | 133, 296, 320, 415 |
| XapI | RAATTY | 1 cut(s) | 345 |
| XceI | RCATGY | 3 cut(s) | 122, 217, 944 |
| XhoI | CTCGAG | 1 cut(s) | 481 |
| XmaI | CCCGGG | 1 cut(s) | 831 |
| XmaJI | CCTAGG | 1 cut(s) | 887 |
| XmiI | GTMKAC | 2 cut(s) | 123, 236 |
| XspI | CTAG | 3 cut(s) | 888, 959, 1030 |
| ZraI | GACGTC | 1 cut(s) | 243 |