RchiOBHm_Chr2g0087931

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002341
Physical Location & Seq
Reverse (-)
2618232 .. 2620635
2404 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46332

Sequence Viewer

Length: 1284 bp
ATGAATAAATTGGAAGGTATTGTTCACTGGATAAAGAAGCAACTTGAACCGTTGTTTGGTCCATTGAGCAGCGATGACATCTCCAGGTTTTTGTGCATGCACCATGTGCAAAAACTGGATATTCCTTTTATTGCAATGTACTTGAAAGAGGACTGCCTTACCTTATTGAAAGATCATCCTGAACAACCTGAAGAAGAAAAATCTAAAGTAAAGTGGTACAGGATTCTGTGGGCCGCTCAGGAATTGCACAAAAAGTGGTTGCTGTTTCAGAATCAGAAAAGGGCTTTGCGATTTTCCTACAATAAGCGCTTTGAGGAAGAATCTCGACTTGGACTCATACATGATAAGGCGGCTCCACAGCTTTTTGAATCAATTATCAACTCCCTCCAAGCTGCAGAGTCACAGAGGGAGGTTGAGGATATCAACAGCAAGTTTAACTTGCATTTCCCTGCAACTGAGGGTCCGGCTACTATTGTTATCTGTAGTGATCCAGGTGTCAGTAGAGCTAAAGACTCGTTACTTACGGAATATGGAAGGGCTTTGTTCAATAAAGTCTCTGTGGCACCGCCATATCAACGGAATGAAACTGCACCACCCAGGGTCATGGCTTGCTGTTGGGGCCCTGGGGACCCTGCAACAACTTTAGTGATGTTAGATTCATGTGGGGAGATTCTAGATGTACTTTGCACCGGGCCTCTCAGCTGGGAGTCTCTTACCTGGACCATACCCTTCTTTGATGAAGCTACTCAACAGCGTATGAATTGTGATCAAGAACGTATTTTGAAGTTTGTGACTGATCACCAACCTGATGTCACTATCATAGGAGCAGCTAATATGTCTTGTCCTCTCCTGAGAGAACAACTGCTTTGGGTGAGTGATACAATGATGATGAAACCTAACAGTAGAGCAAGCCCCCTAAGGATTGTGTATGGGGATGAATCTCTGGCTCGCCTATATGAGATTTCTGAGATTTCATCGGAGCAGTTACCTGGACGATCAGGCATTGCCAGGCGGGCTGTGGCTCTAGGGCGTTATCTTCAAAATCCATTGGCAATGGTTGCCACATTGTGTGGAGCTGGAAGAGAAATCCTATCTTGGAAACTACTCAATTCTCCTGATGAGAACTATCTCAACCCGGACGAGAAGTATGCAGTGATTGAACAGGTCATGATAGATGTGACAAACCAGGTTGGCCTAGACATAAATTTGGCTATAAACCATGAATGGTTATTTGCACCTTTGCAATTTATTTCTGGGCTTGGACCTCAAAAGCAGCATCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

427

Amino Acids

48.55

Weight (kDa)

5.58

Isoelectric Point (pI)

52.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YqgF PF14639 201 - 357 3.2e-13 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 363 - 425 1.6e-08 Helix-hairpin-helix motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 1 cut(s) 236
AciI CCGC 4 cut(s) 234, 350, 566, 1012
AclWI GGATC 1 cut(s) 482
AcsI RAATTY 1 cut(s) 1204
AcuI CTGAAG 1 cut(s) 210
AdeI CACNNNGTG 2 cut(s) 106, 1068
AfaI GTAC 3 cut(s) 140, 218, 681
AfeI AGCGCT 1 cut(s) 308
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 8 cut(s) 47, 145, 169, 368, 547, 784, 1040, 1160
AjnI CCWGG 8 cut(s) 83, 490, 596, 622, 716, 988, 1007, 1185
AjuI GAANNNNNNNTTGG 4 cut(s) 39, 71, 312, 344
AluBI AGCT 7 cut(s) 361, 392, 506, 702, 743, 830, 1076
AluI AGCT 7 cut(s) 361, 392, 506, 702, 743, 830, 1076
Alw26I GTCTC 2 cut(s) 559, 714
AlwI GGATC 1 cut(s) 482
Aor51HI AGCGCT 1 cut(s) 308
AoxI GGCC 4 cut(s) 231, 619, 692, 1192
ApaI GGGCCC 1 cut(s) 623
ApeKI GCWGC 4 cut(s) 69, 392, 827, 1273
ApoI RAATTY 1 cut(s) 1204
AspLEI GCGC 1 cut(s) 309
AspS9I GGNCC 9 cut(s) 59, 231, 461, 619, 620, 628, 692, 720, 1262
AsuC2I CCSGG 2 cut(s) 691, 1136
AsuHPI GGTGA 2 cut(s) 791, 883
AvaII GGWCC 5 cut(s) 59, 461, 628, 720, 1262
AxyI CCTNAGG 1 cut(s) 917
BaeGI GKGCMC 1 cut(s) 623
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 623
BbvI GCAGC 3 cut(s) 81, 379, 839
BcgI CGANNNNNNTGC 2 cut(s) 1130, 1164
BciT130I CCWGG 8 cut(s) 85, 492, 598, 624, 718, 990, 1009, 1187
BclI TGATCA 2 cut(s) 766, 796
BcnI CCSGG 2 cut(s) 691, 1136
BcoDI GTCTC 2 cut(s) 559, 714
BfaI CTAG 3 cut(s) 674, 1025, 1196
BfmI CTRYAG 2 cut(s) 393, 481
BfoI RGCGCY 1 cut(s) 310
BglI GCCNNNNNGGC 1 cut(s) 1013
BisI GCNGC 6 cut(s) 70, 234, 351, 393, 828, 1274
BlsI GCNGC 6 cut(s) 71, 235, 352, 394, 829, 1275
Bme18I GGWCC 5 cut(s) 59, 461, 628, 720, 1262
BmgT120I GGNCC 9 cut(s) 59, 231, 461, 619, 620, 628, 692, 720, 1262
BmiI GGNNCC 7 cut(s) 354, 462, 564, 620, 621, 629, 630
BpmI CTGGAG 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 237
BpuMI CCSGG 2 cut(s) 691, 1136
BsaJI CCNNGG 4 cut(s) 596, 597, 622, 623
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse1I ACTGG 2 cut(s) 32, 120
Bse21I CCTNAGG 1 cut(s) 917
Bse3DI GCAATG 3 cut(s) 141, 1002, 1059
BseBI CCWGG 8 cut(s) 85, 492, 598, 624, 718, 990, 1009, 1187
BseDI CCNNGG 4 cut(s) 596, 597, 622, 623
BseGI GGATG 2 cut(s) 175, 940
BseLI CCNNNNNNNGG 1 cut(s) 56
BseMI GCAATG 3 cut(s) 141, 1002, 1059
BseMII CTCAG 5 cut(s) 251, 447, 712, 842, 957
BseNI ACTGG 2 cut(s) 32, 120
BseSI GKGCMC 1 cut(s) 623
BseXI GCAGC 3 cut(s) 81, 379, 839
BseYI CCCAGC 1 cut(s) 702
BsgI GTGCAG 1 cut(s) 573
BshFI GGCC 4 cut(s) 233, 621, 694, 1194
BshNI GGYRCC 1 cut(s) 562
BsiSI CCGG 3 cut(s) 464, 690, 1136
BslFI GGGAC 1 cut(s) 641
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 2 cut(s) 559, 714
BsmFI GGGAC 1 cut(s) 641
BsnI GGCC 4 cut(s) 233, 621, 694, 1194
Bsp120I GGGCCC 1 cut(s) 619
Bsp1286I GDGCHC 1 cut(s) 623
Bsp143I GATC 5 cut(s) 172, 487, 766, 796, 995
BspACI CCGC 4 cut(s) 234, 350, 566, 1012
BspANI GGCC 4 cut(s) 233, 621, 694, 1194
BspCNI CTCAG 5 cut(s) 250, 448, 711, 843, 958
BspHI TCATGA 1 cut(s) 1167
BspLI GGNNCC 7 cut(s) 354, 462, 564, 620, 621, 629, 630
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 1 cut(s) 482
BspT107I GGYRCC 1 cut(s) 562
BsrBI CCGCTC 1 cut(s) 236
BsrDI GCAATG 3 cut(s) 141, 1002, 1059
BsrI ACTGG 2 cut(s) 32, 120
BssECI CCNNGG 4 cut(s) 596, 597, 622, 623
BssMI GATC 5 cut(s) 172, 487, 766, 796, 995
Bst2UI CCWGG 8 cut(s) 85, 492, 598, 624, 718, 990, 1009, 1187
Bst4CI ACNGT 2 cut(s) 51, 902
Bst6I CTCTTC 1 cut(s) 1075
BstAPI GCANNNNNTGC 2 cut(s) 106, 1058
BstC8I GCNNGC 5 cut(s) 98, 610, 910, 949, 1014
BstDEI CTNAG 6 cut(s) 237, 456, 698, 851, 917, 966
BstF5I GGATG 2 cut(s) 175, 940
BstH2I RGCGCY 1 cut(s) 310
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 5 cut(s) 175, 490, 769, 799, 998
BstMAI GTCTC 2 cut(s) 559, 714
BstMBI GATC 5 cut(s) 172, 487, 766, 796, 995
BstMWI GCNNNNNNNGC 4 cut(s) 106, 618, 1013, 1058
BstNI CCWGG 8 cut(s) 85, 492, 598, 624, 718, 990, 1009, 1187
BstNSI RCATGY 1 cut(s) 100
BstSFI CTRYAG 2 cut(s) 393, 481
BstSLI GKGCMC 1 cut(s) 623
BstV1I GCAGC 3 cut(s) 81, 379, 839
BstXI CCANNNNNNTGG 1 cut(s) 604
Bsu36I CCTNAGG 1 cut(s) 917
BsuRI GGCC 4 cut(s) 233, 621, 694, 1194
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 2 cut(s) 175, 940
BtsI GCAGTG 1 cut(s) 1158
BtsIMutI CAGTG 2 cut(s) 25, 1158
Cac8I GCNNGC 5 cut(s) 98, 610, 910, 949, 1014
CciI TCATGA 1 cut(s) 1167
CfoI GCGC 1 cut(s) 309
Cfr13I GGNCC 9 cut(s) 59, 231, 461, 619, 620, 628, 692, 720, 1262
CsiI ACCWGGT 1 cut(s) 1185
Csp6I GTAC 3 cut(s) 139, 217, 680
CviAII CATG 7 cut(s) 97, 104, 341, 604, 660, 1168, 1220
CviQI GTAC 3 cut(s) 139, 217, 680
DdeI CTNAG 6 cut(s) 237, 456, 698, 851, 917, 966
DpnI GATC 5 cut(s) 174, 489, 768, 798, 997
DpnII GATC 5 cut(s) 172, 487, 766, 796, 995
DraIII CACNNNGTG 2 cut(s) 106, 1068
Eam1104I CTCTTC 1 cut(s) 1075
EarI CTCTTC 1 cut(s) 1075
Eco24I GRGCYC 1 cut(s) 623
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 5 cut(s) 59, 461, 628, 720, 1262
Eco47III AGCGCT 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 210
Eco81I CCTNAGG 1 cut(s) 917
EcoO109I RGGNCCY 3 cut(s) 619, 620, 628
EcoRII CCWGG 8 cut(s) 83, 490, 596, 622, 716, 988, 1007, 1185
EcoRV GATATC 1 cut(s) 421
EcoT38I GRGCYC 1 cut(s) 623
FaeI CATG 7 cut(s) 100, 107, 344, 607, 663, 1171, 1223
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 1 cut(s) 641
FatI CATG 7 cut(s) 96, 103, 340, 603, 659, 1167, 1219
FauI CCCGC 1 cut(s) 1005
FbaI TGATCA 2 cut(s) 766, 796
Fnu4HI GCNGC 6 cut(s) 70, 234, 351, 393, 828, 1274
FokI GGATG 2 cut(s) 162, 947
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 6 cut(s) 70, 234, 351, 393, 828, 1274
FspBI CTAG 3 cut(s) 674, 1025, 1196
GlaI GCGC 1 cut(s) 308
GluI GCNGC 6 cut(s) 70, 234, 351, 393, 828, 1274
GsaI CCCAGC 1 cut(s) 706
GsuI CTGGAG 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 310
HaeIII GGCC 4 cut(s) 233, 621, 694, 1194
HapII CCGG 3 cut(s) 464, 690, 1136
HhaI GCGC 1 cut(s) 309
Hin1II CATG 7 cut(s) 100, 107, 344, 607, 663, 1171, 1223
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HpaII CCGG 3 cut(s) 464, 690, 1136
HphI GGTGA 2 cut(s) 791, 883
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 5 cut(s) 270, 276, 967, 979, 1283
Hpy188III TCNNGA 8 cut(s) 179, 239, 324, 674, 770, 850, 1115, 1168
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 3 cut(s) 8, 528, 739
HpyCH4III ACNGT 2 cut(s) 51, 902
HpyCH4IV ACGT 1 cut(s) 775
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 618, 1013, 1058
HpyF3I CTNAG 6 cut(s) 237, 456, 698, 851, 917, 966
HpySE526I ACGT 1 cut(s) 775
Hsp92II CATG 7 cut(s) 100, 107, 344, 607, 663, 1171, 1223
HspAI GCGC 1 cut(s) 307
KflI GGGWCCC 1 cut(s) 628
Ksp22I TGATCA 2 cut(s) 766, 796
Kzo9I GATC 5 cut(s) 172, 487, 766, 796, 995
LmnI GCTCC 4 cut(s) 358, 824, 979, 1073
Lsp1109I GCAGC 3 cut(s) 81, 379, 839
MabI ACCWGGT 1 cut(s) 1185
MaeI CTAG 3 cut(s) 674, 1025, 1196
MaeII ACGT 1 cut(s) 775
MaeIII GTNAC 6 cut(s) 399, 516, 790, 811, 984, 1177
MalI GATC 5 cut(s) 174, 489, 768, 798, 997
MbiI CCGCTC 1 cut(s) 236
MboI GATC 5 cut(s) 172, 487, 766, 796, 995
MboII GAAGA 5 cut(s) 203, 206, 329, 1028, 1092
MhlI GDGCHC 1 cut(s) 623
MluCI AATT 7 cut(s) 8, 242, 372, 760, 1108, 1204, 1244
MlyI GAGTC 4 cut(s) 327, 407, 506, 716
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 702
MspI CCGG 3 cut(s) 464, 690, 1136
MvaI CCWGG 8 cut(s) 85, 492, 598, 624, 718, 990, 1009, 1187
MwoI GCNNNNNNNGC 4 cut(s) 106, 618, 1013, 1058
NciI CCSGG 2 cut(s) 691, 1136
NdeII GATC 5 cut(s) 172, 487, 766, 796, 995
NlaIII CATG 7 cut(s) 100, 107, 344, 607, 663, 1171, 1223
NlaIV GGNNCC 7 cut(s) 354, 462, 564, 620, 621, 629, 630
NmuCI GTSAC 4 cut(s) 399, 790, 811, 1177
NspI RCATGY 1 cut(s) 100
PaeI GCATGC 1 cut(s) 100
PagI TCATGA 1 cut(s) 1167
PasI CCCWGGG 2 cut(s) 597, 623
PfeI GAWTC 7 cut(s) 223, 271, 320, 368, 656, 670, 938
PkrI GCNGC 6 cut(s) 71, 235, 352, 394, 829, 1275
PleI GAGTC 4 cut(s) 327, 406, 506, 715
PpsI GAGTC 4 cut(s) 327, 406, 506, 715
PpuMI RGGWCCY 1 cut(s) 628
Psp5II RGGWCCY 1 cut(s) 628
Psp6I CCWGG 8 cut(s) 83, 490, 596, 622, 716, 988, 1007, 1185
PspFI CCCAGC 1 cut(s) 702
PspGI CCWGG 8 cut(s) 83, 490, 596, 622, 716, 988, 1007, 1185
PspN4I GGNNCC 7 cut(s) 354, 462, 564, 620, 621, 629, 630
PspOMI GGGCCC 1 cut(s) 619
PspPI GGNCC 9 cut(s) 59, 231, 461, 619, 620, 628, 692, 720, 1262
PspPPI RGGWCCY 1 cut(s) 628
PstI CTGCAG 1 cut(s) 397
PvuII CAGCTG 1 cut(s) 702
RsaI GTAC 3 cut(s) 140, 218, 681
RsaNI GTAC 3 cut(s) 139, 217, 680
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 6 cut(s) 70, 234, 351, 393, 828, 1274
Sau3AI GATC 5 cut(s) 172, 487, 766, 796, 995
Sau96I GGNCC 9 cut(s) 59, 231, 461, 619, 620, 628, 692, 720, 1262
SchI GAGTC 4 cut(s) 327, 407, 506, 716
SduI GDGCHC 1 cut(s) 623
SexAI ACCWGGT 1 cut(s) 1185
SfcI CTRYAG 2 cut(s) 393, 481
SinI GGWCC 5 cut(s) 59, 461, 628, 720, 1262
SphI GCATGC 1 cut(s) 100
Sse9I AATT 7 cut(s) 8, 242, 372, 760, 1108, 1204, 1244
SsiI CCGC 4 cut(s) 234, 350, 566, 1012
SspMI CTAG 3 cut(s) 674, 1025, 1196
TaaI ACNGT 2 cut(s) 51, 902
TaiI ACGT 1 cut(s) 778
TaqI TCGA 1 cut(s) 325
TasI AATT 7 cut(s) 8, 242, 372, 760, 1108, 1204, 1244
TatI WGTACW 2 cut(s) 138, 679
TauI GCSGC 2 cut(s) 236, 353
TfiI GAWTC 7 cut(s) 223, 271, 320, 368, 656, 670, 938
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 2 cut(s) 32, 1158
TseFI GTSAC 4 cut(s) 399, 790, 811, 1177
TseI GCWGC 4 cut(s) 69, 392, 827, 1273
Tsp45I GTSAC 4 cut(s) 399, 790, 811, 1177
TspDTI ATGAA 9 cut(s) 17, 597, 648, 753, 773, 905, 951, 963, 1236
TspGWI ACGGA 2 cut(s) 539, 592
TspRI CASTG 2 cut(s) 32, 1158
VpaK11BI GGWCC 5 cut(s) 59, 461, 628, 720, 1262
XapI RAATTY 1 cut(s) 1204
XbaI TCTAGA 1 cut(s) 673
XceI RCATGY 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 1 cut(s) 1015
XspI CTAG 3 cut(s) 674, 1025, 1196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.