Rh2CG029400

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002341
Physical Location & Seq
Forward (+)
2208436 .. 2210237
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG029400.1

Sequence Viewer

Length: 1179 bp
ATGGCTCCGGCATATCAACATAAGGAAAATGAGATTAAGTCAGATGATGAAGCTTCTGCTTCACCGAGGGTCATGGCTTGCTGTTGGGGTCAAGCTGAAGGTACTCTAGTGATGTTAGATTCATATGGGGAATTGCTAGATGTGCTATGCATAGGTTCCTTTTCATGGGAGTCTTTCAACTACACTGAAAACTGCAATGATACTGTATTACTGTTACTTGGCTCCATAATTATTATGAAAAAAAAACGGGTCTTGAAGTTCGTGACTGATCACCAACCAAGTGTTGTGGTTATCGGAGCTGTAAGAGGAGAAGTGAGTGAAAAGACTAAAGCTTTGTTTCCCTTCAGGATTGTTTATGGGGATGAATCTTTGGGTCTCCTGTATGAAAATTCTCAGACCTCTTCCAATCAGCTACGTACCTGGGCAATCAGTTCCCCTTTTGAGGACATTCTCACTCCGGACGAGAAGTATAAGGTGATTCAACAGGTCATGGTAGATGTTACAAACCAAGTAGCAGGCCTAGACATAAATTTGGCCATAAGCCATGAATGGTTATTTGCACCTTTGCAATTCATTTCCGGACTTGGACCTAGAAAGGCAGCACATTTGCAGCAGTGGTTGGTCAGATCTGGATGCACAATCTCTAATCGGAGGGAGTTTGTTACATTGCACCAGCTTGGTCAAAAGGTGTATGATAATGCAGTCAGTTTCTTGCGTGTTCAGCTGAGCAGCAGGGGCGGCAAGCAATTTCGTGATCTGTTGGATGATACAAGAATACATCCAGAATCCTATATTCTTGCTGAGGAATTGGTGAAAGTCGTTTATGATGGTAACGATGCTCTAGAGTGCATTTGGTATCGACCTGGTTACCTTGAAAGGCTTGACATTGAAGCATATGCTAGAAGCAAGCAACTTGAGGATAAGATGCAAACTTTGTATGATATAAGGAAAGAATTAATACAGGGTTTTCGGGACCCGAGGAAAGAGTATGAAGAACCAAGTGTTAATGTACTTTTTCATCTGCTCACTGGTTTAACTGATGATGCCATTTGTGAAGGAAGAATTGTGCAGGCAACGGTGTACAGCGTGTGTCCTATTAGGGCATCTTGTAATACTGAACTTGGATTGAGGGTGCGCTTCGGAAAGCAGACTTTTCAGATCATGGAAGGCGTACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

392

Amino Acids

44.51

Weight (kDa)

5.35

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHH_7 PF14635 145 - 240 8.7e-18 Helix-hairpin-helix motif
HHH_9 PF17674 253 - 327 1.4e-08 HHH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 457, 578
AciI CCGC 1 cut(s) 738
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 2 cut(s) 388, 529
AcuI CTGAAG 2 cut(s) 117, 328
AfaI GTAC 5 cut(s) 103, 418, 1011, 1082, 1173
AfiI CCNNNNNNNGG 2 cut(s) 165, 442
AgsI TTSAA 5 cut(s) 178, 256, 482, 875, 890
AjnI CCWGG 2 cut(s) 419, 862
AluBI AGCT 7 cut(s) 53, 95, 299, 332, 412, 676, 724
AluI AGCT 7 cut(s) 53, 95, 299, 332, 412, 676, 724
Alw26I GTCTC 1 cut(s) 380
Ama87I CYCGRG 1 cut(s) 976
Aor13HI TCCGGA 2 cut(s) 457, 578
AoxI GGCC 2 cut(s) 517, 534
ApeKI GCWGC 3 cut(s) 599, 610, 729
ApoI RAATTY 2 cut(s) 388, 529
ArsI GACNNNNNNTTYG 2 cut(s) 316, 348
AseI ATTAAT 1 cut(s) 956
AspLEI GCGC 1 cut(s) 1137
AspS9I GGNCC 2 cut(s) 587, 973
AsuHPI GGTGA 4 cut(s) 54, 263, 487, 823
AvaI CYCGRG 1 cut(s) 976
AvaII GGWCC 2 cut(s) 587, 973
BaeI ACNNNNGTAYC 2 cut(s) 93, 126
BalI TGGCCA 1 cut(s) 536
BbvCI CCTCAGC 1 cut(s) 801
BbvI GCAGC 3 cut(s) 611, 622, 741
BccI CCATC 1 cut(s) 821
BciT130I CCWGG 2 cut(s) 421, 864
BclI TGATCA 1 cut(s) 268
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 6 cut(s) 107, 137, 521, 591, 842, 900
BfmI CTRYAG 1 cut(s) 1175
BglII AGATCT 1 cut(s) 626
BisI GCNGC 4 cut(s) 600, 611, 730, 739
BlpI GCTNAGC 1 cut(s) 725
BlsI GCNGC 4 cut(s) 601, 612, 731, 740
Bme1390I CCNGG 2 cut(s) 421, 864
Bme18I GGWCC 2 cut(s) 587, 973
BmeT110I CYCGRG 1 cut(s) 976
BmgT120I GGNCC 2 cut(s) 587, 973
BmiI GGNNCC 5 cut(s) 6, 157, 223, 974, 975
BmrFI CCNGG 2 cut(s) 421, 864
BmsI GCATC 5 cut(s) 623, 826, 915, 1033, 1112
Bpu10I CCTNAGC 1 cut(s) 801
Bpu1102I GCTNAGC 1 cut(s) 725
BpuEI CTTGAG 1 cut(s) 935
BsaAI YACGTR 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 65, 420, 977
BsaWI WCCGGW 2 cut(s) 457, 578
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 442
Bse1I ACTGG 1 cut(s) 1033
Bse3DI GCAATG 2 cut(s) 202, 665
BseAI TCCGGA 2 cut(s) 457, 578
BseBI CCWGG 2 cut(s) 421, 864
BseDI CCNNGG 3 cut(s) 65, 420, 977
BseGI GGATG 4 cut(s) 367, 638, 769, 778
BseLI CCNNNNNNNGG 2 cut(s) 165, 442
BseMI GCAATG 2 cut(s) 202, 665
BseMII CTCAG 3 cut(s) 407, 716, 792
BseNI ACTGG 1 cut(s) 1033
BseRI GAGGAG 1 cut(s) 321
BseXI GCAGC 3 cut(s) 611, 622, 741
BsgI GTGCAG 1 cut(s) 1088
BshFI GGCC 2 cut(s) 519, 536
BsiHKCI CYCGRG 1 cut(s) 976
BsiSI CCGG 3 cut(s) 8, 458, 579
BslFI GGGAC 1 cut(s) 986
BslI CCNNNNNNNGG 2 cut(s) 165, 442
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 1 cut(s) 986
BsnI GGCC 2 cut(s) 519, 536
Bso31I GGTCTC 1 cut(s) 380
BsoBI CYCGRG 1 cut(s) 976
Bsp13I TCCGGA 2 cut(s) 457, 578
Bsp1407I TGTACA 1 cut(s) 1080
Bsp143I GATC 4 cut(s) 268, 626, 754, 1158
Bsp1720I GCTNAGC 1 cut(s) 725
BspACI CCGC 1 cut(s) 738
BspANI GGCC 2 cut(s) 519, 536
BspCNI CTCAG 3 cut(s) 406, 717, 793
BspEI TCCGGA 2 cut(s) 457, 578
BspLI GGNNCC 5 cut(s) 6, 157, 223, 974, 975
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 2 cut(s) 202, 665
BsrGI TGTACA 1 cut(s) 1080
BsrI ACTGG 1 cut(s) 1033
BssECI CCNNGG 3 cut(s) 65, 420, 977
BssMI GATC 4 cut(s) 268, 626, 754, 1158
Bst2UI CCWGG 2 cut(s) 421, 864
Bst4CI ACNGT 3 cut(s) 205, 213, 1078
Bst6I CTCTTC 1 cut(s) 406
BstAUI TGTACA 1 cut(s) 1080
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 5 cut(s) 79, 517, 743, 908, 1071
BstDEI CTNAG 3 cut(s) 393, 725, 801
BstEII GGTNACC 1 cut(s) 866
BstF5I GGATG 4 cut(s) 367, 638, 769, 778
BstHHI GCGC 1 cut(s) 1137
BstKTI GATC 4 cut(s) 271, 629, 757, 1161
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 4 cut(s) 268, 626, 754, 1158
BstMWI GCNNNNNNNGC 4 cut(s) 142, 721, 735, 738
BstNI CCWGG 2 cut(s) 421, 864
BstPI GGTNACC 1 cut(s) 866
BstSCI CCNGG 2 cut(s) 419, 862
BstSFI CTRYAG 1 cut(s) 1175
BstSNI TACGTA 1 cut(s) 416
BstV1I GCAGC 3 cut(s) 611, 622, 741
BstX2I RGATCY 1 cut(s) 626
BstYI RGATCY 1 cut(s) 626
BsuRI GGCC 2 cut(s) 519, 536
BtsCI GGATG 4 cut(s) 367, 638, 769, 778
BtsI GCAGTG 1 cut(s) 620
BtsIMutI CAGTG 3 cut(s) 183, 620, 1026
Cac8I GCNNGC 5 cut(s) 79, 517, 743, 908, 1071
CfoI GCGC 1 cut(s) 1137
Cfr13I GGNCC 2 cut(s) 587, 973
CsiI ACCWGGT 1 cut(s) 862
Csp6I GTAC 5 cut(s) 102, 417, 1010, 1081, 1172
CspCI CAANNNNNGTGG 2 cut(s) 267, 302
CviAII CATG 5 cut(s) 73, 165, 490, 545, 1162
CviQI GTAC 5 cut(s) 102, 417, 1010, 1081, 1172
DdeI CTNAG 3 cut(s) 393, 725, 801
DpnI GATC 4 cut(s) 270, 628, 756, 1160
DpnII GATC 4 cut(s) 268, 626, 754, 1158
EaeI YGGCCR 1 cut(s) 534
Eam1104I CTCTTC 1 cut(s) 406
EarI CTCTTC 1 cut(s) 406
Eco105I TACGTA 1 cut(s) 416
Eco147I AGGCCT 1 cut(s) 519
Eco31I GGTCTC 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 587, 973
Eco57I CTGAAG 2 cut(s) 117, 328
Eco88I CYCGRG 1 cut(s) 976
Eco91I GGTNACC 1 cut(s) 866
EcoO109I RGGNCCY 1 cut(s) 973
EcoO65I GGTNACC 1 cut(s) 866
EcoRII CCWGG 2 cut(s) 419, 862
EcoT22I ATGCAT 1 cut(s) 152
FaeI CATG 5 cut(s) 76, 168, 493, 548, 1165
FaqI GGGAC 1 cut(s) 986
FatI CATG 5 cut(s) 72, 164, 489, 544, 1161
FauNDI CATATG 2 cut(s) 124, 895
FbaI TGATCA 1 cut(s) 268
Fnu4HI GCNGC 4 cut(s) 600, 611, 730, 739
FokI GGATG 4 cut(s) 374, 645, 765, 776
Fsp4HI GCNGC 4 cut(s) 600, 611, 730, 739
FspBI CTAG 6 cut(s) 107, 137, 521, 591, 842, 900
GlaI GCGC 1 cut(s) 1136
GluI GCNGC 4 cut(s) 600, 611, 730, 739
HaeIII GGCC 2 cut(s) 519, 536
HapII CCGG 3 cut(s) 8, 458, 579
HhaI GCGC 1 cut(s) 1137
Hin1II CATG 5 cut(s) 76, 168, 493, 548, 1165
Hin6I GCGC 1 cut(s) 1135
HinP1I GCGC 1 cut(s) 1135
HindIII AAGCTT 2 cut(s) 51, 330
HinfI GANTC 5 cut(s) 119, 170, 365, 478, 785
HpaII CCGG 3 cut(s) 8, 458, 579
HphI GGTGA 4 cut(s) 54, 263, 487, 823
Hpy166II GTNNAC 1 cut(s) 1081
Hpy188I TCNGA 7 cut(s) 43, 296, 396, 626, 651, 1142, 1158
Hpy8I GTNNAC 1 cut(s) 1081
HpyAV CCTTC 4 cut(s) 92, 352, 1049, 1160
HpyCH4III ACNGT 3 cut(s) 205, 213, 1078
HpyCH4IV ACGT 1 cut(s) 415
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 721, 735, 738
HpyF3I CTNAG 3 cut(s) 393, 725, 801
HpySE526I ACGT 1 cut(s) 415
Hsp92II CATG 5 cut(s) 76, 168, 493, 548, 1165
HspAI GCGC 1 cut(s) 1135
KflI GGGWCCC 1 cut(s) 973
Kpn2I TCCGGA 2 cut(s) 457, 578
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 4 cut(s) 268, 626, 754, 1158
LmnI GCTCC 3 cut(s) 10, 227, 296
Lsp1109I GCAGC 3 cut(s) 611, 622, 741
LweI GCATC 5 cut(s) 623, 826, 915, 1033, 1112
MabI ACCWGGT 1 cut(s) 862
MaeI CTAG 6 cut(s) 107, 137, 521, 591, 842, 900
MaeII ACGT 1 cut(s) 415
MaeIII GTNAC 6 cut(s) 213, 262, 499, 661, 830, 866
MalI GATC 4 cut(s) 270, 628, 756, 1160
MboI GATC 4 cut(s) 268, 626, 754, 1158
MboII GAAGA 3 cut(s) 393, 1004, 1071
MflI RGATCY 1 cut(s) 626
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 9 cut(s) 131, 228, 388, 529, 569, 746, 806, 953, 1062
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 741
MnlI CCTC 9 cut(s) 60, 299, 409, 436, 645, 796, 910, 972, 1122
Mox20I TGGCCA 1 cut(s) 536
Mph1103I ATGCAT 1 cut(s) 152
MroI TCCGGA 2 cut(s) 457, 578
MscI TGGCCA 1 cut(s) 536
MseI TTAA 4 cut(s) 36, 956, 1005, 1034
Msp20I TGGCCA 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 724
MspI CCGG 3 cut(s) 8, 458, 579
MspR9I CCNGG 2 cut(s) 421, 864
MvaI CCWGG 2 cut(s) 421, 864
MwoI GCNNNNNNNGC 4 cut(s) 142, 721, 735, 738
NdeI CATATG 2 cut(s) 124, 895
NdeII GATC 4 cut(s) 268, 626, 754, 1158
NlaIII CATG 5 cut(s) 76, 168, 493, 548, 1165
NlaIV GGNNCC 5 cut(s) 6, 157, 223, 974, 975
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 152
PceI AGGCCT 1 cut(s) 519
PfeI GAWTC 4 cut(s) 119, 365, 478, 785
PkrI GCNGC 4 cut(s) 601, 612, 731, 740
PleI GAGTC 1 cut(s) 178
PpsI GAGTC 1 cut(s) 178
Ppu21I YACGTR 1 cut(s) 416
PpuMI RGGWCCY 1 cut(s) 973
PshBI ATTAAT 1 cut(s) 956
Psp5II RGGWCCY 1 cut(s) 973
Psp6I CCWGG 2 cut(s) 419, 862
PspEI GGTNACC 1 cut(s) 866
PspGI CCWGG 2 cut(s) 419, 862
PspN4I GGNNCC 5 cut(s) 6, 157, 223, 974, 975
PspPI GGNCC 2 cut(s) 587, 973
PspPPI RGGWCCY 1 cut(s) 973
PsuI RGATCY 1 cut(s) 626
PvuII CAGCTG 1 cut(s) 724
RsaI GTAC 5 cut(s) 103, 418, 1011, 1082, 1173
RsaNI GTAC 5 cut(s) 102, 417, 1010, 1081, 1172
SaqAI TTAA 4 cut(s) 36, 956, 1005, 1034
SatI GCNGC 4 cut(s) 600, 611, 730, 739
Sau3AI GATC 4 cut(s) 268, 626, 754, 1158
Sau96I GGNCC 2 cut(s) 587, 973
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 2 cut(s) 421, 864
SexAI ACCWGGT 1 cut(s) 862
SfaNI GCATC 5 cut(s) 623, 826, 915, 1033, 1112
SfcI CTRYAG 1 cut(s) 1175
SinI GGWCC 2 cut(s) 587, 973
SmlI CTYRAG 1 cut(s) 914
SmoI CTYRAG 1 cut(s) 914
SnaBI TACGTA 1 cut(s) 416
Sse9I AATT 9 cut(s) 131, 228, 388, 529, 569, 746, 806, 953, 1062
SseBI AGGCCT 1 cut(s) 519
SsiI CCGC 1 cut(s) 738
SspMI CTAG 6 cut(s) 107, 137, 521, 591, 842, 900
StuI AGGCCT 1 cut(s) 519
StyD4I CCNGG 2 cut(s) 419, 862
TaaI ACNGT 3 cut(s) 205, 213, 1078
TaiI ACGT 1 cut(s) 418
TaqI TCGA 1 cut(s) 859
TasI AATT 9 cut(s) 131, 228, 388, 529, 569, 746, 806, 953, 1062
TatI WGTACW 2 cut(s) 1009, 1080
TauI GCSGC 1 cut(s) 741
TfiI GAWTC 4 cut(s) 119, 365, 478, 785
Tru1I TTAA 4 cut(s) 36, 956, 1005, 1034
Tru9I TTAA 4 cut(s) 36, 956, 1005, 1034
TscAI CASTG 3 cut(s) 190, 620, 1033
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 3 cut(s) 599, 610, 729
Tsp45I GTSAC 1 cut(s) 262
TspRI CASTG 3 cut(s) 190, 620, 1033
VpaK11BI GGWCC 2 cut(s) 587, 973
VspI ATTAAT 1 cut(s) 956
XapI RAATTY 2 cut(s) 388, 529
XbaI TCTAGA 1 cut(s) 841
XspI CTAG 6 cut(s) 107, 137, 521, 591, 842, 900
Zsp2I ATGCAT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.