Rroxscaffold_2G00153120

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002341
Physical Location & Seq
Forward (+)
89916625 .. 89919562
2938 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153120.1

Sequence Viewer

Length: 1161 bp
ATGGACGTCGATCACGATCACGATCACGGCTACGGTTCTCATTTGGTTAGAATGGAAGCGGCGGACGTCGCCTACGGATTTCGGCCTCGTGATGTACCCGAGAGACCGATATACGATGACGAAAGCACACCGGAAGAAATTAGCATAGATGATGAGAGTCACCGGATACGAAGCAACTTGAACTCGTTGTTTGGTCCATTGAGCAGCAATGACATCTCCACGTTTTTGTACATGCACCATGTGGAAAAAGCGGATGTGAGTGATACAATGATGATGAAACCTAACGATAGAGCAAGCCCCCTAAGGATTGTGTATGGGGATGAATCTCGGCTCGCCTATATGAGAATTCGGAGATTTCATCGAGCGTTACTGGACGATCGTCTTATCATCGAGCATTGCCGAAAGCGGGCCGTGGCTCTAGGGCGTTATCTTCAAAATCCATTGGCAATGGTTGCCACATTGTGTGGACCTGGAAGAGAAATCCTATCTTGGAAACTACTCAATTCTCCCGATGAGAACTATCTCAACCCGGACGAGAAGTATGCGGTGATTGAACAGGTCATGATAGATGTGACAAACCAGGTTGGCCTAGACATAAATTTGGCTATAAACCATGAATGGTTATTTGCACCTTTGCAATTTATTTCGGGCTTGGACCACAAACGGCAGCATCTCTCGAAGAAGTCATTGGTAAAATCTGGTAAAGTATCTACTCGAAAGGAGTTTGCAACATTACATGGGATGGGTGCAAGGGTGTATGTTAATGCGAACAAATCGCCCAAGATGTTTGTGGTGACATGGATGTTCGGTGCGAGCATTAGGGATCTACCTAGTTACCTTGAGAAGCTTGACATCGAAGCATATACTAGAAGCAAAGGATTGCATTATATGATGCAAACATTGTGCTGTATAAGGGAAGAGTTAATACAAGGGTTCCAAGAGTGGCGTAAAAAATATGAAGAACTAAGTGATGATGACATGTTCTATATGATGACCGGTGAAACTCGCGACACCCTCTCGGAAGGAAGCATTGTCCGTGCCACAATTCGCAGGTGCTTTCCAAAACAGCAATTTGTACTTGTGGGAGCTGGGTTGATTTGTTTGCTCGGAGAGACGACTTTAAAGTTGATGGGAGGGAAATTTCAGACTTATCACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

386

Amino Acids

44.48

Weight (kDa)

6.57

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HHH_7 PF14635 161 - 256 8.9e-11 Helix-hairpin-helix motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1041
AatII GACGTC 2 cut(s) 9, 69
Acc36I ACCTGC 1 cut(s) 1041
AccII CGCG 1 cut(s) 1008
AciI CCGC 5 cut(s) 59, 62, 251, 406, 545
AclWI GGATC 1 cut(s) 831
AcsI RAATTY 3 cut(s) 345, 598, 1139
AcyI GRCGYC 2 cut(s) 6, 66
AdeI CACNNNGTG 2 cut(s) 241, 462
AfaI GTAC 3 cut(s) 96, 230, 1077
AfiI CCNNNNNNNGG 1 cut(s) 406
AflIII ACRYGT 1 cut(s) 978
AgeI ACCGGT 1 cut(s) 995
AgsI TTSAA 3 cut(s) 181, 434, 554
AjnI CCWGG 2 cut(s) 469, 579
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AluBI AGCT 2 cut(s) 847, 1088
AluI AGCT 2 cut(s) 847, 1088
Alw26I GTCTC 2 cut(s) 97, 1106
AlwI GGATC 1 cut(s) 831
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 3 cut(s) 83, 408, 586
ApeKI GCWGC 2 cut(s) 204, 667
ApoI RAATTY 3 cut(s) 345, 598, 1139
AsiGI ACCGGT 1 cut(s) 995
AspS9I GGNCC 4 cut(s) 194, 408, 467, 655
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 4 cut(s) 152, 559, 805, 1010
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 3 cut(s) 194, 467, 655
AxyI CCTNAGG 1 cut(s) 302
BarI GAAGNNNNNNTAC 2 cut(s) 909, 941
BauI CACGAG 1 cut(s) 87
BbvI GCAGC 2 cut(s) 216, 679
BccI CCATC 2 cut(s) 736, 1123
BceAI ACGGC 3 cut(s) 43, 395, 680
BcgI CGANNNNNNTGC 2 cut(s) 524, 558
BciT130I CCWGG 2 cut(s) 471, 581
BciVI GTATCC 1 cut(s) 159
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 2 cut(s) 97, 1106
BfaI CTAG 4 cut(s) 419, 590, 831, 867
BfuAI ACCTGC 1 cut(s) 1041
BfuI GTATCC 1 cut(s) 159
BisI GCNGC 3 cut(s) 60, 205, 668
BlsI GCNGC 3 cut(s) 61, 206, 669
Bme1390I CCNGG 3 cut(s) 471, 530, 581
Bme18I GGWCC 3 cut(s) 194, 467, 655
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 4 cut(s) 194, 408, 467, 655
BmiI GGNNCC 1 cut(s) 935
BmrFI CCNGG 3 cut(s) 471, 530, 581
BmsI GCATC 2 cut(s) 679, 882
BoxI GACNNNNGTC 1 cut(s) 378
BpuEI CTTGAG 1 cut(s) 860
BpuMI CCSGG 1 cut(s) 530
BsaBI GATNNNNATC 2 cut(s) 15, 21
BsaHI GRCGYC 2 cut(s) 6, 66
BsaI GGTCTC 1 cut(s) 97
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 3 cut(s) 130, 162, 995
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse118I RCCGGY 1 cut(s) 995
Bse1I ACTGG 1 cut(s) 375
Bse21I CCTNAGG 1 cut(s) 302
Bse3DI GCAATG 3 cut(s) 214, 394, 453
Bse8I GATNNNNATC 2 cut(s) 15, 21
BseBI CCWGG 2 cut(s) 471, 581
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 4 cut(s) 259, 325, 747, 807
BseJI GATNNNNATC 2 cut(s) 15, 21
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMI GCAATG 3 cut(s) 214, 394, 453
BseNI ACTGG 1 cut(s) 375
BseXI GCAGC 2 cut(s) 216, 679
BseYI CCCAGC 1 cut(s) 1088
Bsh1236I CGCG 1 cut(s) 1008
Bsh1285I CGRYCG 1 cut(s) 379
BshFI GGCC 3 cut(s) 85, 410, 588
BshTI ACCGGT 1 cut(s) 995
BsiEI CGRYCG 1 cut(s) 379
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 4 cut(s) 131, 163, 530, 996
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 2 cut(s) 97, 1106
BsmBI CGTCTC 1 cut(s) 1106
BsnI GGCC 3 cut(s) 85, 410, 588
Bso31I GGTCTC 1 cut(s) 97
BsoBI CYCGRG 1 cut(s) 98
Bsp1407I TGTACA 1 cut(s) 228
Bsp143I GATC 5 cut(s) 10, 16, 22, 376, 823
Bsp68I TCGCGA 1 cut(s) 1008
BspACI CCGC 5 cut(s) 59, 62, 251, 406, 545
BspANI GGCC 3 cut(s) 85, 410, 588
BspFNI CGCG 1 cut(s) 1008
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 1 cut(s) 935
BspMI ACCTGC 1 cut(s) 1041
BspPI GGATC 1 cut(s) 831
BspTNI GGTCTC 1 cut(s) 97
BsrDI GCAATG 3 cut(s) 214, 394, 453
BsrFI RCCGGY 1 cut(s) 995
BsrGI TGTACA 1 cut(s) 228
BsrI ACTGG 1 cut(s) 375
BssAI RCCGGY 1 cut(s) 995
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 5 cut(s) 10, 16, 22, 376, 823
BssNI GRCGYC 2 cut(s) 6, 66
BssSI CACGAG 1 cut(s) 87
Bst2BI CACGAG 1 cut(s) 87
Bst2UI CCWGG 2 cut(s) 471, 581
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 2 cut(s) 469, 912
BstACI GRCGYC 2 cut(s) 6, 66
BstAPI GCANNNNNTGC 1 cut(s) 452
BstAUI TGTACA 1 cut(s) 228
BstC8I GCNNGC 4 cut(s) 295, 333, 408, 814
BstDEI CTNAG 2 cut(s) 302, 965
BstDSI CCRYGG 1 cut(s) 411
BstF5I GGATG 4 cut(s) 259, 325, 747, 807
BstFNI CGCG 1 cut(s) 1008
BstKTI GATC 5 cut(s) 13, 19, 25, 379, 826
BstMAI GTCTC 2 cut(s) 97, 1106
BstMBI GATC 5 cut(s) 10, 16, 22, 376, 823
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 2 cut(s) 68, 452
BstNI CCWGG 2 cut(s) 471, 581
BstNSI RCATGY 2 cut(s) 235, 982
BstPAI GACNNNNGTC 1 cut(s) 378
BstSCI CCNGG 3 cut(s) 469, 528, 579
BstUI CGCG 1 cut(s) 1008
BstV1I GCAGC 2 cut(s) 216, 679
BstX2I RGATCY 1 cut(s) 823
BstYI RGATCY 1 cut(s) 823
Bsu36I CCTNAGG 1 cut(s) 302
BsuI GTATCC 1 cut(s) 159
BsuRI GGCC 3 cut(s) 85, 410, 588
BtgI CCRYGG 1 cut(s) 411
BtsCI GGATG 4 cut(s) 259, 325, 747, 807
BtuMI TCGCGA 1 cut(s) 1008
BveI ACCTGC 1 cut(s) 1041
Cac8I GCNNGC 4 cut(s) 295, 333, 408, 814
CciI TCATGA 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 995
Cfr13I GGNCC 4 cut(s) 194, 408, 467, 655
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 3 cut(s) 95, 229, 1076
CspAI ACCGGT 1 cut(s) 995
CviAII CATG 7 cut(s) 232, 239, 562, 614, 737, 798, 979
CviQI GTAC 3 cut(s) 95, 229, 1076
DdeI CTNAG 2 cut(s) 302, 965
DpnI GATC 5 cut(s) 12, 18, 24, 378, 825
DpnII GATC 5 cut(s) 10, 16, 22, 376, 823
DraI TTTAAA 1 cut(s) 1122
DraIII CACNNNGTG 2 cut(s) 241, 462
Eam1104I CTCTTC 2 cut(s) 469, 912
EarI CTCTTC 2 cut(s) 469, 912
EciI GGCGGA 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 97
Eco47I GGWCC 3 cut(s) 194, 467, 655
Eco81I CCTNAGG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 2 cut(s) 469, 579
Esp3I CGTCTC 1 cut(s) 1106
FaeI CATG 7 cut(s) 235, 242, 565, 617, 740, 801, 982
FatI CATG 7 cut(s) 231, 238, 561, 613, 736, 797, 978
FauI CCCGC 1 cut(s) 399
Fnu4HI GCNGC 3 cut(s) 60, 205, 668
FokI GGATG 4 cut(s) 266, 332, 754, 814
Fsp4HI GCNGC 3 cut(s) 60, 205, 668
FspBI CTAG 4 cut(s) 419, 590, 831, 867
GluI GCNGC 3 cut(s) 60, 205, 668
GsaI CCCAGC 1 cut(s) 1092
HaeIII GGCC 3 cut(s) 85, 410, 588
HapII CCGG 4 cut(s) 131, 163, 530, 996
Hin1I GRCGYC 2 cut(s) 6, 66
Hin1II CATG 7 cut(s) 235, 242, 565, 617, 740, 801, 982
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 2 cut(s) 157, 323
HpaII CCGG 4 cut(s) 131, 163, 530, 996
HphI GGTGA 4 cut(s) 152, 559, 805, 1010
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 4 cut(s) 351, 1021, 1109, 1146
Hpy188III TCNNGA 7 cut(s) 14, 20, 89, 509, 562, 676, 1007
Hpy8I GTNNAC 1 cut(s) 467
Hpy99I CGWCG 2 cut(s) 11, 71
HpyAV CCTTC 1 cut(s) 1016
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4IV ACGT 3 cut(s) 6, 66, 221
HpyCH4V TGCA 7 cut(s) 235, 629, 637, 728, 749, 883, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 452
HpyF3I CTNAG 2 cut(s) 302, 965
HpySE526I ACGT 3 cut(s) 6, 66, 221
Hsp92I GRCGYC 2 cut(s) 6, 66
Hsp92II CATG 7 cut(s) 235, 242, 565, 617, 740, 801, 982
Kzo9I GATC 5 cut(s) 10, 16, 22, 376, 823
LmnI GCTCC 1 cut(s) 1085
Lsp1109I GCAGC 2 cut(s) 216, 679
LweI GCATC 2 cut(s) 679, 882
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 4 cut(s) 419, 590, 831, 867
MaeII ACGT 3 cut(s) 6, 66, 221
MaeIII GTNAC 5 cut(s) 158, 366, 571, 793, 833
MalI GATC 5 cut(s) 12, 18, 24, 378, 825
MboI GATC 5 cut(s) 10, 16, 22, 376, 823
MboII GAAGA 6 cut(s) 146, 422, 486, 691, 929, 971
MflI RGATCY 1 cut(s) 823
MluCI AATT 8 cut(s) 138, 345, 502, 598, 638, 1044, 1070, 1139
MlyI GAGTC 1 cut(s) 166
MnlI CCTC 3 cut(s) 96, 1025, 1127
MseI TTAA 3 cut(s) 762, 923, 1121
MspI CCGG 4 cut(s) 131, 163, 530, 996
MspR9I CCNGG 3 cut(s) 471, 530, 581
MvaI CCWGG 2 cut(s) 471, 581
MvnI CGCG 1 cut(s) 1008
MwoI GCNNNNNNNGC 2 cut(s) 68, 452
NciI CCSGG 1 cut(s) 530
NdeII GATC 5 cut(s) 10, 16, 22, 376, 823
NlaIII CATG 7 cut(s) 235, 242, 565, 617, 740, 801, 982
NlaIV GGNNCC 1 cut(s) 935
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 3 cut(s) 158, 571, 793
NruI TCGCGA 1 cut(s) 1008
NspI RCATGY 2 cut(s) 235, 982
PagI TCATGA 1 cut(s) 561
PaqCI CACCTGC 1 cut(s) 1041
PciI ACATGT 1 cut(s) 978
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PfeI GAWTC 1 cut(s) 323
PinAI ACCGGT 1 cut(s) 995
PkrI GCNGC 3 cut(s) 61, 206, 669
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 165
PscI ACATGT 1 cut(s) 978
PshAI GACNNNNGTC 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 469, 579
PspFI CCCAGC 1 cut(s) 1088
PspGI CCWGG 2 cut(s) 469, 579
PspN4I GGNNCC 1 cut(s) 935
PspPI GGNCC 4 cut(s) 194, 408, 467, 655
PsuI RGATCY 1 cut(s) 823
PvuI CGATCG 1 cut(s) 379
RruI TCGCGA 1 cut(s) 1008
RsaI GTAC 3 cut(s) 96, 230, 1077
RsaNI GTAC 3 cut(s) 95, 229, 1076
SaqAI TTAA 3 cut(s) 762, 923, 1121
SatI GCNGC 3 cut(s) 60, 205, 668
Sau3AI GATC 5 cut(s) 10, 16, 22, 376, 823
Sau96I GGNCC 4 cut(s) 194, 408, 467, 655
SchI GAGTC 1 cut(s) 166
ScrFI CCNGG 3 cut(s) 471, 530, 581
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 2 cut(s) 679, 882
SinI GGWCC 3 cut(s) 194, 467, 655
SmlI CTYRAG 1 cut(s) 839
SmoI CTYRAG 1 cut(s) 839
Sse9I AATT 8 cut(s) 138, 345, 502, 598, 638, 1044, 1070, 1139
SsiI CCGC 5 cut(s) 59, 62, 251, 406, 545
SspMI CTAG 4 cut(s) 419, 590, 831, 867
StyD4I CCNGG 3 cut(s) 469, 528, 579
TaaI ACNGT 1 cut(s) 35
TaiI ACGT 3 cut(s) 9, 69, 224
TaqI TCGA 6 cut(s) 9, 361, 390, 677, 715, 855
TaqII GACCGA 1 cut(s) 121
TasI AATT 8 cut(s) 138, 345, 502, 598, 638, 1044, 1070, 1139
TatI WGTACW 2 cut(s) 228, 1075
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 3 cut(s) 762, 923, 1121
Tru9I TTAA 3 cut(s) 762, 923, 1121
TseFI GTSAC 3 cut(s) 158, 571, 793
TseI GCWGC 2 cut(s) 204, 667
Tsp45I GTSAC 3 cut(s) 158, 571, 793
TspDTI ATGAA 5 cut(s) 290, 336, 347, 630, 972
TspGWI ACGGA 2 cut(s) 90, 1025
VpaK11BI GGWCC 3 cut(s) 194, 467, 655
XapI RAATTY 3 cut(s) 345, 598, 1139
XceI RCATGY 2 cut(s) 235, 982
XcmI CCANNNNNNNNNTGG 1 cut(s) 787
XspI CTAG 4 cut(s) 419, 590, 831, 867
ZraI GACGTC 2 cut(s) 7, 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.