Rh2DG029200

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002341
Physical Location & Seq
Forward (+)
1768893 .. 1770501
1609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG029200.1

Sequence Viewer

Length: 960 bp
ATGGCTCCGGCATATCAACACAAGGAAAATGAGATTAAGTCAGATGATGAAGCTTCTGCTTCACCGAGGGTCATGGCTTGCTGTTGGGGTCAAGCTGAAGGTACTCTAGTGATGTACGTAAGAGGAGAAGTGAGTGAAAAGACTAAAGCTTTGTTTCCCTTCAGGATTGTTTATGGGGATGAATCTTTGGGTCGCCTGTATGAAAATTCTCAGACCTCTTCCAATCAGCTACCTGGGCAATCAGACAATGTCAAGCGGGCAGTGGCTCTTGGGCGTTATCTTCAAAATCCACTAGCAATGGTTGCCACACTCTGCGGACCTGGAAGAGATATCTTTTCCCCTTTTGAGGACATTCTCACTCCGGACGAGAAGTATAAGGTGATTGAACAGGTCATGGTAGATGTTACAAACCACGTAGGCCTAGACATAAATTTGGCCACAAGCCATGAATGGTTATTTGCACCTTTGCAATTCATTTCCGGACTTGGACCTAGAAAGCCAGCACATTTGCAGCAGTGGTTGGTCAGATCTGGATGCACAATCTCTAATCGGAGGGAGTTTGTTACATTGCACCAGCTTGGTCAAAAGGTGTATGATAATGCAGTCAGTTTCTTGCGTGTTCAGCTGAGCAGCAGGAGCGGCAAGCAATTTCGTGATCTGTTGGATGATACAAGAATACATCCAGAATCCTATATTCTTGCTGAGGAATTGGTGAAAGTCGTTTATGATGGTAACGATGCTCTAGAGTGCATTTGGTACCGACCTGGTTACCTTGAAAGGCTTGACATTGAAGCATATGCTAGAAGCAAGCAACTTGAGGATAAGATGCAAACTTTGTATGATATAAGGAAAGAATTAATACAGGGTTTTCGGGACCCGAGGAAAGAGTATGAAGAACCAAGTGATAATGTACTTTTTCATCTGCTCACTGGTTTAACTGATGATGCCGGCCATTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

319

Amino Acids

36.33

Weight (kDa)

5.37

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YqgF PF14639 43 - 105 1.4e-06 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 112 - 207 2.5e-19 Helix-hairpin-helix motif
HHH_9 PF17674 220 - 294 9.8e-09 HHH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 756
AccB1I GGYRCC 1 cut(s) 756
AccBSI CCGCTC 1 cut(s) 639
AccIII TCCGGA 2 cut(s) 361, 479
AciI CCGC 3 cut(s) 256, 315, 639
AcoI YGGCCR 2 cut(s) 435, 949
AcsI RAATTY 2 cut(s) 205, 430
AcuI CTGAAG 2 cut(s) 117, 145
AfaI GTAC 4 cut(s) 103, 116, 758, 912
AfiI CCNNNNNNNGG 1 cut(s) 346
AgsI TTSAA 4 cut(s) 284, 386, 776, 791
AjnI CCWGG 3 cut(s) 232, 319, 763
AluBI AGCT 6 cut(s) 53, 95, 149, 229, 577, 625
AluI AGCT 6 cut(s) 53, 95, 149, 229, 577, 625
Ama87I CYCGRG 1 cut(s) 877
Aor13HI TCCGGA 2 cut(s) 361, 479
AoxI GGCC 3 cut(s) 418, 435, 949
ApeKI GCWGC 2 cut(s) 511, 630
ApoI RAATTY 2 cut(s) 205, 430
ArsI GACNNNNNNTTYG 2 cut(s) 133, 165
AseI ATTAAT 1 cut(s) 857
Asp718I GGTACC 1 cut(s) 756
AspS9I GGNCC 3 cut(s) 317, 488, 874
AsuHPI GGTGA 3 cut(s) 54, 391, 724
AvaI CYCGRG 1 cut(s) 877
AvaII GGWCC 3 cut(s) 317, 488, 874
BaeI ACNNNNGTAYC 2 cut(s) 93, 126
BalI TGGCCA 1 cut(s) 437
BanI GGYRCC 1 cut(s) 756
BbvCI CCTCAGC 1 cut(s) 702
BbvI GCAGC 2 cut(s) 523, 642
BccI CCATC 1 cut(s) 722
BciT130I CCWGG 3 cut(s) 234, 321, 765
BfaI CTAG 6 cut(s) 107, 293, 422, 492, 743, 801
BglII AGATCT 1 cut(s) 527
BisI GCNGC 3 cut(s) 512, 631, 640
BlpI GCTNAGC 1 cut(s) 626
BlsI GCNGC 3 cut(s) 513, 632, 641
Bme1390I CCNGG 3 cut(s) 234, 321, 765
Bme18I GGWCC 3 cut(s) 317, 488, 874
BmeT110I CYCGRG 1 cut(s) 877
BmgT120I GGNCC 3 cut(s) 317, 488, 874
BmiI GGNNCC 4 cut(s) 6, 758, 875, 876
BmrFI CCNGG 3 cut(s) 234, 321, 765
BmsI GCATC 4 cut(s) 524, 727, 816, 934
Bpu10I CCTNAGC 1 cut(s) 702
Bpu1102I GCTNAGC 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 836
BsaAI YACGTR 2 cut(s) 118, 415
BsaJI CCNNGG 3 cut(s) 65, 233, 878
BsaWI WCCGGW 2 cut(s) 361, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse118I RCCGGY 1 cut(s) 947
Bse1I ACTGG 1 cut(s) 934
Bse3DI GCAATG 2 cut(s) 303, 566
BseAI TCCGGA 2 cut(s) 361, 479
BseBI CCWGG 3 cut(s) 234, 321, 765
BseDI CCNNGG 3 cut(s) 65, 233, 878
BseGI GGATG 4 cut(s) 184, 539, 670, 679
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 2 cut(s) 303, 566
BseMII CTCAG 3 cut(s) 224, 617, 693
BseNI ACTGG 1 cut(s) 934
BseRI GAGGAG 1 cut(s) 138
BseXI GCAGC 2 cut(s) 523, 642
BshFI GGCC 3 cut(s) 420, 437, 951
BshNI GGYRCC 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 877
BsiSI CCGG 4 cut(s) 8, 362, 480, 948
BslFI GGGAC 1 cut(s) 887
BslI CCNNNNNNNGG 1 cut(s) 346
BsmFI GGGAC 1 cut(s) 887
BsnI GGCC 3 cut(s) 420, 437, 951
BsoBI CYCGRG 1 cut(s) 877
Bsp13I TCCGGA 2 cut(s) 361, 479
Bsp143I GATC 2 cut(s) 527, 655
Bsp1720I GCTNAGC 1 cut(s) 626
BspACI CCGC 3 cut(s) 256, 315, 639
BspANI GGCC 3 cut(s) 420, 437, 951
BspCNI CTCAG 3 cut(s) 223, 618, 694
BspEI TCCGGA 2 cut(s) 361, 479
BspLI GGNNCC 4 cut(s) 6, 758, 875, 876
BspT107I GGYRCC 1 cut(s) 756
BsrBI CCGCTC 1 cut(s) 639
BsrDI GCAATG 2 cut(s) 303, 566
BsrFI RCCGGY 1 cut(s) 947
BsrI ACTGG 1 cut(s) 934
BssAI RCCGGY 1 cut(s) 947
BssECI CCNNGG 3 cut(s) 65, 233, 878
BssMI GATC 2 cut(s) 527, 655
Bst2UI CCWGG 3 cut(s) 234, 321, 765
Bst6I CTCTTC 2 cut(s) 223, 319
BstAPI GCANNNNNTGC 1 cut(s) 302
BstBAI YACGTR 2 cut(s) 118, 415
BstC8I GCNNGC 6 cut(s) 79, 258, 501, 644, 809, 949
BstDEI CTNAG 3 cut(s) 210, 626, 702
BstEII GGTNACC 1 cut(s) 767
BstF5I GGATG 4 cut(s) 184, 539, 670, 679
BstKTI GATC 2 cut(s) 530, 658
BstMBI GATC 2 cut(s) 527, 655
BstMWI GCNNNNNNNGC 5 cut(s) 235, 302, 622, 636, 639
BstNI CCWGG 3 cut(s) 234, 321, 765
BstPI GGTNACC 1 cut(s) 767
BstSCI CCNGG 3 cut(s) 232, 319, 763
BstSNI TACGTA 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 523, 642
BstX2I RGATCY 1 cut(s) 527
BstYI RGATCY 1 cut(s) 527
BsuRI GGCC 3 cut(s) 420, 437, 951
BtsCI GGATG 4 cut(s) 184, 539, 670, 679
BtsI GCAGTG 2 cut(s) 267, 521
BtsIMutI CAGTG 3 cut(s) 267, 521, 927
Cac8I GCNNGC 6 cut(s) 79, 258, 501, 644, 809, 949
Cfr10I RCCGGY 1 cut(s) 947
Cfr13I GGNCC 3 cut(s) 317, 488, 874
CsiI ACCWGGT 1 cut(s) 763
Csp6I GTAC 4 cut(s) 102, 115, 757, 911
CviAII CATG 3 cut(s) 73, 394, 446
CviQI GTAC 4 cut(s) 102, 115, 757, 911
DdeI CTNAG 3 cut(s) 210, 626, 702
DpnI GATC 2 cut(s) 529, 657
DpnII GATC 2 cut(s) 527, 655
EaeI YGGCCR 2 cut(s) 435, 949
Eam1104I CTCTTC 2 cut(s) 223, 319
EarI CTCTTC 2 cut(s) 223, 319
Eco105I TACGTA 1 cut(s) 118
Eco147I AGGCCT 1 cut(s) 420
Eco32I GATATC 1 cut(s) 331
Eco47I GGWCC 3 cut(s) 317, 488, 874
Eco57I CTGAAG 2 cut(s) 117, 145
Eco88I CYCGRG 1 cut(s) 877
Eco91I GGTNACC 1 cut(s) 767
EcoO109I RGGNCCY 1 cut(s) 874
EcoO65I GGTNACC 1 cut(s) 767
EcoRII CCWGG 3 cut(s) 232, 319, 763
EcoRV GATATC 1 cut(s) 331
FaeI CATG 3 cut(s) 76, 397, 449
FaqI GGGAC 1 cut(s) 887
FatI CATG 3 cut(s) 72, 393, 445
FauI CCCGC 1 cut(s) 249
FauNDI CATATG 1 cut(s) 796
Fnu4HI GCNGC 3 cut(s) 512, 631, 640
FokI GGATG 4 cut(s) 191, 546, 666, 677
Fsp4HI GCNGC 3 cut(s) 512, 631, 640
FspBI CTAG 6 cut(s) 107, 293, 422, 492, 743, 801
GluI GCNGC 3 cut(s) 512, 631, 640
HaeIII GGCC 3 cut(s) 420, 437, 951
HapII CCGG 4 cut(s) 8, 362, 480, 948
Hin1II CATG 3 cut(s) 76, 397, 449
HindIII AAGCTT 2 cut(s) 51, 147
HinfI GANTC 2 cut(s) 182, 686
HpaII CCGG 4 cut(s) 8, 362, 480, 948
HphI GGTGA 3 cut(s) 54, 391, 724
Hpy188I TCNGA 5 cut(s) 43, 213, 244, 527, 552
Hpy188III TCNNGA 8 cut(s) 163, 362, 480, 531, 653, 683, 743, 872
HpyAV CCTTC 2 cut(s) 92, 169
HpyCH4IV ACGT 2 cut(s) 117, 414
HpyCH4V TGCA 8 cut(s) 461, 469, 511, 537, 571, 602, 750, 829
HpyF10VI GCNNNNNNNGC 5 cut(s) 235, 302, 622, 636, 639
HpyF3I CTNAG 3 cut(s) 210, 626, 702
HpySE526I ACGT 2 cut(s) 117, 414
Hsp92II CATG 3 cut(s) 76, 397, 449
KflI GGGWCCC 1 cut(s) 874
Kpn2I TCCGGA 2 cut(s) 361, 479
KpnI GGTACC 1 cut(s) 760
KroI GCCGGC 1 cut(s) 947
KroNI GCCGGC 1 cut(s) 949
Kzo9I GATC 2 cut(s) 527, 655
LmnI GCTCC 2 cut(s) 10, 636
Lsp1109I GCAGC 2 cut(s) 523, 642
LweI GCATC 4 cut(s) 524, 727, 816, 934
MabI ACCWGGT 1 cut(s) 763
MaeI CTAG 6 cut(s) 107, 293, 422, 492, 743, 801
MaeII ACGT 2 cut(s) 117, 414
MaeIII GTNAC 4 cut(s) 403, 562, 731, 767
MalI GATC 2 cut(s) 529, 657
MbiI CCGCTC 1 cut(s) 639
MboI GATC 2 cut(s) 527, 655
MboII GAAGA 4 cut(s) 210, 272, 336, 905
MflI RGATCY 1 cut(s) 527
MlsI TGGCCA 1 cut(s) 437
MluCI AATT 6 cut(s) 205, 430, 470, 647, 707, 854
MluNI TGGCCA 1 cut(s) 437
MmeI TCCRAC 1 cut(s) 642
MnlI CCTC 8 cut(s) 60, 116, 226, 340, 546, 697, 811, 873
Mox20I TGGCCA 1 cut(s) 437
MroI TCCGGA 2 cut(s) 361, 479
MroNI GCCGGC 1 cut(s) 947
MscI TGGCCA 1 cut(s) 437
MseI TTAA 3 cut(s) 36, 857, 935
Msp20I TGGCCA 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 625
MspI CCGG 4 cut(s) 8, 362, 480, 948
MspR9I CCNGG 3 cut(s) 234, 321, 765
MvaI CCWGG 3 cut(s) 234, 321, 765
MwoI GCNNNNNNNGC 5 cut(s) 235, 302, 622, 636, 639
NaeI GCCGGC 1 cut(s) 949
NdeI CATATG 1 cut(s) 796
NdeII GATC 2 cut(s) 527, 655
NgoMIV GCCGGC 1 cut(s) 947
NlaIII CATG 3 cut(s) 76, 397, 449
NlaIV GGNNCC 4 cut(s) 6, 758, 875, 876
PceI AGGCCT 1 cut(s) 420
PdiI GCCGGC 1 cut(s) 949
PfeI GAWTC 2 cut(s) 182, 686
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 3 cut(s) 513, 632, 641
Ppu21I YACGTR 2 cut(s) 118, 415
PpuMI RGGWCCY 1 cut(s) 874
PshBI ATTAAT 1 cut(s) 857
Psp5II RGGWCCY 1 cut(s) 874
Psp6I CCWGG 3 cut(s) 232, 319, 763
PspEI GGTNACC 1 cut(s) 767
PspGI CCWGG 3 cut(s) 232, 319, 763
PspN4I GGNNCC 4 cut(s) 6, 758, 875, 876
PspPI GGNCC 3 cut(s) 317, 488, 874
PspPPI RGGWCCY 1 cut(s) 874
PsuI RGATCY 1 cut(s) 527
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 4 cut(s) 103, 116, 758, 912
RsaNI GTAC 4 cut(s) 102, 115, 757, 911
SaqAI TTAA 3 cut(s) 36, 857, 935
SatI GCNGC 3 cut(s) 512, 631, 640
Sau3AI GATC 2 cut(s) 527, 655
Sau96I GGNCC 3 cut(s) 317, 488, 874
ScrFI CCNGG 3 cut(s) 234, 321, 765
SexAI ACCWGGT 1 cut(s) 763
SfaNI GCATC 4 cut(s) 524, 727, 816, 934
SinI GGWCC 3 cut(s) 317, 488, 874
SmlI CTYRAG 1 cut(s) 815
SmoI CTYRAG 1 cut(s) 815
SnaBI TACGTA 1 cut(s) 118
Sse9I AATT 6 cut(s) 205, 430, 470, 647, 707, 854
SseBI AGGCCT 1 cut(s) 420
SsiI CCGC 3 cut(s) 256, 315, 639
SspMI CTAG 6 cut(s) 107, 293, 422, 492, 743, 801
StuI AGGCCT 1 cut(s) 420
StyD4I CCNGG 3 cut(s) 232, 319, 763
TaiI ACGT 2 cut(s) 120, 417
TasI AATT 6 cut(s) 205, 430, 470, 647, 707, 854
TatI WGTACW 1 cut(s) 910
TauI GCSGC 1 cut(s) 642
TfiI GAWTC 2 cut(s) 182, 686
Tru1I TTAA 3 cut(s) 36, 857, 935
Tru9I TTAA 3 cut(s) 36, 857, 935
TscAI CASTG 3 cut(s) 267, 521, 934
TseI GCWGC 2 cut(s) 511, 630
TspDTI ATGAA 7 cut(s) 63, 195, 216, 462, 463, 906, 908
TspRI CASTG 3 cut(s) 267, 521, 934
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 3 cut(s) 317, 488, 874
VspI ATTAAT 1 cut(s) 857
XapI RAATTY 2 cut(s) 205, 430
XbaI TCTAGA 1 cut(s) 742
XspI CTAG 6 cut(s) 107, 293, 422, 492, 743, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.