Rh2BG033400

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002341
Physical Location & Seq
Reverse (-)
2309014 .. 2310627
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG033400.1

Sequence Viewer

Length: 942 bp
ATGGAAGAAGCGCACAAGGAACCATCCACAGTCGATCGGGATGACCAGATTTTGAAGCTTGATTTTTCAGAAACAATGCAGATATACGATGATGAAGAAAGCATACTGGAAGGAATCAGCATACATGATGAGAGTCAGTGGATACATAATCAACTTGAACCTTTGTTTGGTTCAATAAGCAGGGATGACATCTTCAGGTTTTTGCAGATGCACCATGTGCAAAAATTGGATATTCCCTTCATTGCTATGTACCGAAAAGAGGACTGCCTTAGCTTACTGAAAGATCCTGAACAGCCTGAAGAAGAAAAATCTACGTTAACGTGGCACAAGGTACTGTGGGCCATTCAGGAATTGCACAAAAAGTGGCTGCTGTTTCAGAATCAGAAAAGGGCATTGCAATTTCACTACAATAAGCGCTTTGAGGAAGAATCTCGACGAGGACTCTTACATGATGAGACTCCACAGCTTTTTGAATCAATTATGAAGTCACTCCGAGCTGCAGAGTCAGAGAGAGAGGTCGAGGATGTCAACAGCAAGTTTAACTTGCATTTCCCTGCAGGTGAGGGTCAGGCTGCTATTGATATCAGTAGTGATCCAGGTGTGAGGAAGTATGTCCGCAGCAATTACTTTGATTTCGTTGAAGTATCAGTCACGAGCACAAAACCTGGCTTATATATGGATTGTCAAAAAAAGCCATTGAATAGATTTGAGGATGCACAATGGTTGCTTGTCCAGAAGGCTGAAGAGGAGAATCTTGTACAAGTTACTATTAAGCTGCCCGAAGACCGTCTGAACAAGTTGATACGCGACTTAAGCAACTACAATCTTAGTAAATCTGCACAACTGTGGAACGAGCAGCGCAAGTTGATATTGCAAGACGCTCTTTCCAATTTCCTTCTGCCTTCAATGGAGAAAGAAGCAAGATCCTTGTTGGTCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

313

Amino Acids

36.91

Weight (kDa)

5.15

Isoelectric Point (pI)

56.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 548
AccII CGCG 1 cut(s) 807
AciI CCGC 1 cut(s) 616
AclWI GGATC 3 cut(s) 278, 587, 918
AcuI CTGAAG 3 cut(s) 178, 318, 762
AdeI CACNNNGTG 1 cut(s) 217
AfaI GTAC 3 cut(s) 251, 333, 759
AfeI AGCGCT 1 cut(s) 416
AfiI CCNNNNNNNGG 2 cut(s) 167, 259
AflII CTTAAG 1 cut(s) 811
AgsI TTSAA 7 cut(s) 55, 158, 174, 473, 641, 700, 906
AjnI CCWGG 2 cut(s) 595, 664
AjuI GAANNNNNNNTTGG 2 cut(s) 150, 182
AleI CACNNNNGTG 1 cut(s) 844
AluBI AGCT 5 cut(s) 58, 273, 466, 497, 775
AluI AGCT 5 cut(s) 58, 273, 466, 497, 775
Alw21I GWGCWC 1 cut(s) 659
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 3 cut(s) 278, 587, 918
Aor51HI AGCGCT 1 cut(s) 416
AoxI GGCC 1 cut(s) 339
ApeKI GCWGC 6 cut(s) 367, 497, 572, 618, 775, 856
AspLEI GCGC 3 cut(s) 13, 417, 861
AspS9I GGNCC 1 cut(s) 339
AsuHPI GGTGA 1 cut(s) 572
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
BauI CACGAG 1 cut(s) 652
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 1 cut(s) 659
BbvI GCAGC 6 cut(s) 354, 484, 559, 630, 762, 868
BccI CCATC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 597, 666
BciVI GTATCC 1 cut(s) 135
BcoDI GTCTC 1 cut(s) 449
BfmI CTRYAG 2 cut(s) 498, 555
BfoI RGCGCY 1 cut(s) 418
BfrI CTTAAG 1 cut(s) 811
BfuAI ACCTGC 1 cut(s) 548
BfuI GTATCC 1 cut(s) 135
BisI GCNGC 6 cut(s) 368, 498, 573, 619, 776, 857
BlsI GCNGC 6 cut(s) 369, 499, 574, 620, 777, 858
Bme1390I CCNGG 2 cut(s) 597, 666
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 2 cut(s) 597, 666
BmsI GCATC 2 cut(s) 198, 703
BpiI GAAGAC 1 cut(s) 789
Bpu10I CCTNAGC 1 cut(s) 269
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 259
Bse1I ACTGG 1 cut(s) 111
Bse3DI GCAATG 2 cut(s) 240, 392
BseBI CCWGG 2 cut(s) 597, 666
BseGI GGATG 5 cut(s) 23, 46, 190, 529, 718
BseLI CCNNNNNNNGG 2 cut(s) 167, 259
BseMI GCAATG 2 cut(s) 240, 392
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 1 cut(s) 761
BseXI GCAGC 6 cut(s) 354, 484, 559, 630, 762, 868
BsgI GTGCAG 1 cut(s) 822
Bsh1236I CGCG 1 cut(s) 807
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 1 cut(s) 341
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 659
BslI CCNNNNNNNGG 2 cut(s) 167, 259
BsmAI GTCTC 1 cut(s) 449
BsnI GGCC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 659
Bsp1407I TGTACA 1 cut(s) 757
Bsp143I GATC 4 cut(s) 34, 283, 592, 923
BspACI CCGC 1 cut(s) 616
BspANI GGCC 1 cut(s) 341
BspFNI CGCG 1 cut(s) 807
BspLI GGNNCC 1 cut(s) 21
BspMAI CTGCAG 2 cut(s) 502, 559
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 3 cut(s) 278, 587, 918
BspTI CTTAAG 1 cut(s) 811
BsrDI GCAATG 2 cut(s) 240, 392
BsrGI TGTACA 1 cut(s) 757
BsrI ACTGG 1 cut(s) 111
BssMI GATC 4 cut(s) 34, 283, 592, 923
BssSI CACGAG 1 cut(s) 652
Bst2BI CACGAG 1 cut(s) 652
Bst2UI CCWGG 2 cut(s) 597, 666
Bst4CI ACNGT 4 cut(s) 31, 336, 788, 846
Bst6I CTCTTC 1 cut(s) 738
BstAFI CTTAAG 1 cut(s) 811
BstAPI GCANNNNNTGC 1 cut(s) 217
BstAUI TGTACA 1 cut(s) 757
BstDEI CTNAG 2 cut(s) 269, 827
BstF5I GGATG 5 cut(s) 23, 46, 190, 529, 718
BstFNI CGCG 1 cut(s) 807
BstH2I RGCGCY 1 cut(s) 418
BstHHI GCGC 3 cut(s) 13, 417, 861
BstKTI GATC 4 cut(s) 37, 286, 595, 926
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 4 cut(s) 34, 283, 592, 923
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 2 cut(s) 217, 813
BstNI CCWGG 2 cut(s) 597, 666
BstSCI CCNGG 2 cut(s) 595, 664
BstSFI CTRYAG 2 cut(s) 498, 555
BstUI CGCG 1 cut(s) 807
BstV1I GCAGC 6 cut(s) 354, 484, 559, 630, 762, 868
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 2 cut(s) 283, 923
BstYI RGATCY 2 cut(s) 283, 923
BsuI GTATCC 1 cut(s) 135
BsuRI GGCC 1 cut(s) 341
BtsCI GGATG 5 cut(s) 23, 46, 190, 529, 718
BtsIMutI CAGTG 1 cut(s) 143
BveI ACCTGC 1 cut(s) 548
CfoI GCGC 3 cut(s) 13, 417, 861
Cfr13I GGNCC 1 cut(s) 339
CseI GACGC 1 cut(s) 887
Csp6I GTAC 3 cut(s) 250, 332, 758
CviAII CATG 3 cut(s) 125, 215, 449
CviQI GTAC 3 cut(s) 250, 332, 758
DdeI CTNAG 2 cut(s) 269, 827
DpnI GATC 4 cut(s) 36, 285, 594, 925
DpnII GATC 4 cut(s) 34, 283, 592, 923
DraIII CACNNNGTG 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 738
EarI CTCTTC 1 cut(s) 738
Eco32I GATATC 1 cut(s) 583
Eco47III AGCGCT 1 cut(s) 416
Eco57I CTGAAG 3 cut(s) 178, 318, 762
EcoRII CCWGG 2 cut(s) 595, 664
EcoRV GATATC 1 cut(s) 583
FaeI CATG 3 cut(s) 128, 218, 452
FalI AAGNNNNNCTT 4 cut(s) 527, 559, 867, 899
FatI CATG 3 cut(s) 124, 214, 448
Fnu4HI GCNGC 6 cut(s) 368, 498, 573, 619, 776, 857
FokI GGATG 5 cut(s) 10, 53, 197, 536, 725
Fsp4HI GCNGC 6 cut(s) 368, 498, 573, 619, 776, 857
GlaI GCGC 3 cut(s) 12, 416, 860
GluI GCNGC 6 cut(s) 368, 498, 573, 619, 776, 857
HaeII RGCGCY 1 cut(s) 418
HaeIII GGCC 1 cut(s) 341
HgaI GACGC 1 cut(s) 887
HhaI GCGC 3 cut(s) 13, 417, 861
Hin1II CATG 3 cut(s) 128, 218, 452
Hin6I GCGC 3 cut(s) 11, 415, 859
HinP1I GCGC 3 cut(s) 11, 415, 859
HincII GTYRAC 2 cut(s) 318, 529
HindII GTYRAC 2 cut(s) 318, 529
HindIII AAGCTT 1 cut(s) 56
HinfI GANTC 9 cut(s) 114, 133, 379, 428, 441, 457, 473, 503, 751
HpaI GTTAAC 1 cut(s) 318
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 2 cut(s) 318, 529
Hpy188I TCNGA 6 cut(s) 70, 378, 384, 494, 508, 792
Hpy188III TCNNGA 6 cut(s) 38, 287, 347, 432, 652, 733
Hpy8I GTNNAC 2 cut(s) 318, 529
Hpy99I CGWCG 1 cut(s) 438
HpyAV CCTTC 5 cut(s) 104, 247, 730, 905, 912
HpyCH4III ACNGT 4 cut(s) 31, 336, 788, 846
HpyCH4IV ACGT 2 cut(s) 314, 320
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 813
HpyF3I CTNAG 2 cut(s) 269, 827
HpySE526I ACGT 2 cut(s) 314, 320
Hsp92II CATG 3 cut(s) 128, 218, 452
HspAI GCGC 3 cut(s) 11, 415, 859
KspAI GTTAAC 1 cut(s) 318
Kzo9I GATC 4 cut(s) 34, 283, 592, 923
Lsp1109I GCAGC 6 cut(s) 354, 484, 559, 630, 762, 868
LweI GCATC 2 cut(s) 198, 703
MaeII ACGT 2 cut(s) 314, 320
MaeIII GTNAC 3 cut(s) 486, 649, 763
MalI GATC 4 cut(s) 36, 285, 594, 925
MboI GATC 4 cut(s) 34, 283, 592, 923
MboII GAAGA 8 cut(s) 17, 107, 184, 311, 314, 437, 755, 794
MflI RGATCY 2 cut(s) 283, 923
MhlI GDGCHC 1 cut(s) 659
MluCI AATT 6 cut(s) 224, 350, 398, 477, 622, 889
MlyI GAGTC 4 cut(s) 142, 435, 451, 512
MnlI CCTC 9 cut(s) 253, 415, 431, 508, 514, 556, 597, 703, 739
MseI TTAA 4 cut(s) 317, 540, 771, 812
MslI CAYNNNNRTG 2 cut(s) 245, 844
MspCI CTTAAG 1 cut(s) 811
MspR9I CCNGG 2 cut(s) 597, 666
MvaI CCWGG 2 cut(s) 597, 666
MvnI CGCG 1 cut(s) 807
MwoI GCNNNNNNNGC 2 cut(s) 217, 813
NdeII GATC 4 cut(s) 34, 283, 592, 923
NlaIII CATG 3 cut(s) 128, 218, 452
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 2 cut(s) 486, 649
OliI CACNNNNGTG 1 cut(s) 844
PaqCI CACCTGC 1 cut(s) 548
PfeI GAWTC 5 cut(s) 114, 379, 428, 473, 751
PkrI GCNGC 6 cut(s) 369, 499, 574, 620, 777, 858
Ple19I CGATCG 1 cut(s) 37
PleI GAGTC 4 cut(s) 141, 435, 451, 511
PpsI GAGTC 4 cut(s) 141, 435, 451, 511
Psp6I CCWGG 2 cut(s) 595, 664
PspGI CCWGG 2 cut(s) 595, 664
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 1 cut(s) 339
PstI CTGCAG 2 cut(s) 502, 559
PsuI RGATCY 2 cut(s) 283, 923
PvuI CGATCG 1 cut(s) 37
RsaI GTAC 3 cut(s) 251, 333, 759
RsaNI GTAC 3 cut(s) 250, 332, 758
RseI CAYNNNNRTG 2 cut(s) 245, 844
SaqAI TTAA 4 cut(s) 317, 540, 771, 812
SatI GCNGC 6 cut(s) 368, 498, 573, 619, 776, 857
Sau3AI GATC 4 cut(s) 34, 283, 592, 923
Sau96I GGNCC 1 cut(s) 339
SbfI CCTGCAGG 1 cut(s) 559
SchI GAGTC 4 cut(s) 142, 435, 451, 512
ScrFI CCNGG 2 cut(s) 597, 666
SdaI CCTGCAGG 1 cut(s) 559
SduI GDGCHC 1 cut(s) 659
SfaNI GCATC 2 cut(s) 198, 703
SfcI CTRYAG 2 cut(s) 498, 555
SmiMI CAYNNNNRTG 2 cut(s) 245, 844
SmlI CTYRAG 1 cut(s) 811
SmoI CTYRAG 1 cut(s) 811
Sse8387I CCTGCAGG 1 cut(s) 559
Sse9I AATT 6 cut(s) 224, 350, 398, 477, 622, 889
SsiI CCGC 1 cut(s) 616
StyD4I CCNGG 2 cut(s) 595, 664
TaaI ACNGT 4 cut(s) 31, 336, 788, 846
TaiI ACGT 2 cut(s) 317, 323
TaqI TCGA 3 cut(s) 33, 433, 519
TasI AATT 6 cut(s) 224, 350, 398, 477, 622, 889
TatI WGTACW 1 cut(s) 757
TfiI GAWTC 5 cut(s) 114, 379, 428, 473, 751
Tru1I TTAA 4 cut(s) 317, 540, 771, 812
Tru9I TTAA 4 cut(s) 317, 540, 771, 812
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 2 cut(s) 486, 649
TseI GCWGC 6 cut(s) 367, 497, 572, 618, 775, 856
Tsp45I GTSAC 2 cut(s) 486, 649
TspDTI ATGAA 3 cut(s) 108, 229, 497
TspRI CASTG 1 cut(s) 143
Vha464I CTTAAG 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.