Rh2AG034100

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002341
Physical Location & Seq
Reverse (-)
2476221 .. 2477300
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG034100.1

Sequence Viewer

Length: 1080 bp
ATGGCTTGCTGTTGGGGTCCCGGGGACCCTGCAACCACTTTAGTGATGTTGGATTCATGTGGGGAGCTGCTAGATGTGCTTTGCATAGGGACTATCAGCTGGGAGAATCTCACCTGGACCGTACCATTCTTTGATAAACCTACTAGACTGCGTATGGAAAATGATGATAAACGTATTCTGCAGTTCGTGAATGATCACGAACCTGATCTTGCTATCATAGGAGCGACTAATATGTCTTGTGATATGCTGAAATTACAACTTCTGGGAATTGAATCATTACAGGTACCCGAGAAAATGATCCCCCTAAGGGTTGTATATGGGGATGAAACTTTGGCTTGCCTGTATGAAAATTCTCAGATTTCGGCTGATCAGCTACCTGGACAATCAGGCATGGTCAGGCGGGCTGTGGCTCTTGGGCGTTATCTTCAAAATCCACTGGCAATGGTTGCCACACTTTGTGGACCGGGACGAGAGATCTTATCTTGGAAACCACTCACTTCTCCTTTCGATAACTTTCTCACCCCAGATGAGAAGTACGCGATGATTGAACAGGTCATGGTAGATGTTACAAACCAGGTTGGCCTTGACATAACTTTGGCTACAAGCCATGAATGGTTATTTGCACCTTTGCAATTCATTTCTGGACTTGGACCTAGAAAGGCAGCATCTCTGCAGCGGTCATTGGTAAGAAGTGGTGAAATACTTGCTCGAAGGGAGTTGGCTACGTTGCATGGGCTTGGTAGAAGGTTGTATCTAAATGCAGTTGGTTTCTTGCGTATTCAGCAACATGGGTTAGCTGCTAGCGGCACCAGCAGCCAATTTAATGCTCTGTTGGATGATACAAGAATCCATCCAGAATCCTATTTGCTTGCACAAGAAATGGCCAAAGACGTTTATGATGATAAGGATGCAATGGAGCTGCCGCGCATTAGGGATCAACCTAGTTACCTCGAGAAGCTTGACGTTGAAGCATATGCTAAAAGCAAAAAACTTGAGAATAAGATAAAAACATTGTGCGGCATAAGGAGAGAATTAATACAGGGGTTTCAGGATGAGCTTAAAACAGGAAGAACTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

359

Amino Acids

40.22

Weight (kDa)

5.42

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
YqgF PF14639 2 - 153 8.1e-13 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 167 - 260 2.3e-17 Helix-hairpin-helix motif
HHH_9 PF17674 278 - 347 6.8e-07 HHH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 232
Acc65I GGTACC 1 cut(s) 283
AccB1I GGYRCC 2 cut(s) 283, 806
AccII CGCG 2 cut(s) 539, 925
AciI CCGC 5 cut(s) 400, 676, 804, 923, 1017
AclWI GGATC 2 cut(s) 292, 942
AcoI YGGCCR 1 cut(s) 882
AcsI RAATTY 1 cut(s) 349
AdeI CACNNNGTG 1 cut(s) 458
AfaI GTAC 3 cut(s) 123, 285, 536
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 4 cut(s) 272, 428, 548, 968
AjnI CCWGG 3 cut(s) 113, 376, 573
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 7 cut(s) 67, 99, 373, 797, 919, 958, 1057
AluI AGCT 7 cut(s) 67, 99, 373, 797, 919, 958, 1057
AlwI GGATC 2 cut(s) 292, 942
Ama87I CYCGRG 3 cut(s) 20, 287, 950
AoxI GGCC 2 cut(s) 580, 882
ApeKI GCWGC 6 cut(s) 67, 662, 673, 797, 813, 919
ApoI RAATTY 1 cut(s) 349
AseI ATTAAT 1 cut(s) 1034
Asp718I GGTACC 1 cut(s) 283
AspLEI GCGC 1 cut(s) 927
AspS9I GGNCC 5 cut(s) 17, 25, 117, 461, 650
AsuC2I CCSGG 3 cut(s) 21, 22, 465
AsuHPI GGTGA 3 cut(s) 103, 511, 707
AsuNHI GCTAGC 1 cut(s) 800
AvaI CYCGRG 3 cut(s) 20, 287, 950
AvaII GGWCC 5 cut(s) 17, 25, 117, 461, 650
AxyI CCTNAGG 1 cut(s) 305
BalI TGGCCA 1 cut(s) 884
BanI GGYRCC 2 cut(s) 283, 806
BbvI GCAGC 6 cut(s) 54, 674, 685, 784, 825, 906
BccI CCATC 1 cut(s) 858
BciT130I CCWGG 3 cut(s) 115, 378, 575
BclI TGATCA 2 cut(s) 193, 367
BcnI CCSGG 3 cut(s) 21, 22, 465
BfaI CTAG 6 cut(s) 71, 144, 654, 801, 942, 1074
BfmI CTRYAG 2 cut(s) 179, 671
BglII AGATCT 1 cut(s) 474
BisI GCNGC 9 cut(s) 68, 663, 674, 798, 805, 814, 920, 923, 1018
BlsI GCNGC 9 cut(s) 69, 664, 675, 799, 806, 815, 921, 924, 1019
Bme1390I CCNGG 6 cut(s) 21, 22, 115, 378, 465, 575
Bme18I GGWCC 5 cut(s) 17, 25, 117, 461, 650
BmeT110I CYCGRG 3 cut(s) 20, 287, 950
BmgT120I GGNCC 5 cut(s) 17, 25, 117, 461, 650
BmiI GGNNCC 6 cut(s) 18, 19, 26, 27, 285, 808
BmrFI CCNGG 6 cut(s) 21, 22, 115, 378, 465, 575
BmsI GCATC 2 cut(s) 674, 898
BmtI GCTAGC 1 cut(s) 804
BpuEI CTTGAG 1 cut(s) 1013
BpuMI CCSGG 3 cut(s) 21, 22, 465
BsaJI CCNNGG 2 cut(s) 20, 21
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 441
Bse21I CCTNAGG 1 cut(s) 305
Bse3DI GCAATG 2 cut(s) 447, 918
BseBI CCWGG 3 cut(s) 115, 378, 575
BseDI CCNNGG 2 cut(s) 20, 21
BseGI GGATG 5 cut(s) 328, 841, 850, 913, 1057
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMI GCAATG 2 cut(s) 447, 918
BseMII CTCAG 1 cut(s) 368
BseNI ACTGG 1 cut(s) 441
BseXI GCAGC 6 cut(s) 54, 674, 685, 784, 825, 906
BseYI CCCAGC 1 cut(s) 99
Bsh1236I CGCG 2 cut(s) 539, 925
BshFI GGCC 2 cut(s) 582, 884
BshNI GGYRCC 2 cut(s) 283, 806
BsiHKCI CYCGRG 3 cut(s) 20, 287, 950
BsiSI CCGG 2 cut(s) 21, 464
BslFI GGGAC 4 cut(s) 3, 38, 103, 480
BslI CCNNNNNNNGG 1 cut(s) 307
BsmFI GGGAC 4 cut(s) 3, 38, 103, 480
BsnI GGCC 2 cut(s) 582, 884
BsoBI CYCGRG 3 cut(s) 20, 287, 950
Bsp143I GATC 6 cut(s) 193, 205, 297, 367, 474, 934
BspACI CCGC 5 cut(s) 400, 676, 804, 923, 1017
BspANI GGCC 2 cut(s) 582, 884
BspCNI CTCAG 1 cut(s) 367
BspFNI CGCG 2 cut(s) 539, 925
BspLI GGNNCC 6 cut(s) 18, 19, 26, 27, 285, 808
BspMAI CTGCAG 2 cut(s) 183, 675
BspOI GCTAGC 1 cut(s) 804
BspPI GGATC 2 cut(s) 292, 942
BspT107I GGYRCC 2 cut(s) 283, 806
BsrDI GCAATG 2 cut(s) 447, 918
BsrI ACTGG 1 cut(s) 441
BssECI CCNNGG 2 cut(s) 20, 21
BssMI GATC 6 cut(s) 193, 205, 297, 367, 474, 934
Bst2UI CCWGG 3 cut(s) 115, 378, 575
Bst4CI ACNGT 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 446
BstC8I GCNNGC 5 cut(s) 7, 337, 402, 802, 870
BstDEI CTNAG 2 cut(s) 305, 354
BstF5I GGATG 5 cut(s) 328, 841, 850, 913, 1057
BstFNI CGCG 2 cut(s) 539, 925
BstHHI GCGC 1 cut(s) 927
BstKTI GATC 6 cut(s) 196, 208, 300, 370, 477, 937
BstMBI GATC 6 cut(s) 193, 205, 297, 367, 474, 934
BstMWI GCNNNNNNNGC 5 cut(s) 76, 446, 781, 810, 813
BstNI CCWGG 3 cut(s) 115, 378, 575
BstSCI CCNGG 6 cut(s) 19, 20, 113, 376, 463, 573
BstSFI CTRYAG 2 cut(s) 179, 671
BstUI CGCG 2 cut(s) 539, 925
BstV1I GCAGC 6 cut(s) 54, 674, 685, 784, 825, 906
BstX2I RGATCY 1 cut(s) 474
BstYI RGATCY 1 cut(s) 474
Bsu36I CCTNAGG 1 cut(s) 305
BsuRI GGCC 2 cut(s) 582, 884
BtgZI GCGATG 1 cut(s) 554
BtsCI GGATG 5 cut(s) 328, 841, 850, 913, 1057
BtsIMutI CAGTG 1 cut(s) 434
Cac8I GCNNGC 5 cut(s) 7, 337, 402, 802, 870
CfoI GCGC 1 cut(s) 927
Cfr13I GGNCC 5 cut(s) 17, 25, 117, 461, 650
Cfr9I CCCGGG 1 cut(s) 20
CsiI ACCWGGT 1 cut(s) 573
Csp6I GTAC 3 cut(s) 122, 284, 535
CviAII CATG 6 cut(s) 57, 391, 556, 608, 731, 788
CviQI GTAC 3 cut(s) 122, 284, 535
DdeI CTNAG 2 cut(s) 305, 354
DpnI GATC 6 cut(s) 195, 207, 299, 369, 476, 936
DpnII GATC 6 cut(s) 193, 205, 297, 367, 474, 934
DraIII CACNNNGTG 1 cut(s) 458
DrdI GACNNNNNNGTC 1 cut(s) 232
DseDI GACNNNNNNGTC 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 882
Eco47I GGWCC 5 cut(s) 17, 25, 117, 461, 650
Eco81I CCTNAGG 1 cut(s) 305
Eco88I CYCGRG 3 cut(s) 20, 287, 950
EcoO109I RGGNCCY 2 cut(s) 17, 25
EcoRII CCWGG 3 cut(s) 113, 376, 573
FaeI CATG 6 cut(s) 60, 394, 559, 611, 734, 791
FaqI GGGAC 4 cut(s) 3, 38, 103, 480
FatI CATG 6 cut(s) 56, 390, 555, 607, 730, 787
FauI CCCGC 1 cut(s) 393
FauNDI CATATG 1 cut(s) 973
FbaI TGATCA 2 cut(s) 193, 367
Fnu4HI GCNGC 9 cut(s) 68, 663, 674, 798, 805, 814, 920, 923, 1018
FokI GGATG 5 cut(s) 335, 837, 848, 920, 1064
Fsp4HI GCNGC 9 cut(s) 68, 663, 674, 798, 805, 814, 920, 923, 1018
FspBI CTAG 6 cut(s) 71, 144, 654, 801, 942, 1074
GlaI GCGC 1 cut(s) 926
GluI GCNGC 9 cut(s) 68, 663, 674, 798, 805, 814, 920, 923, 1018
GsaI CCCAGC 1 cut(s) 103
HaeIII GGCC 2 cut(s) 582, 884
HapII CCGG 2 cut(s) 21, 464
HhaI GCGC 1 cut(s) 927
Hin1II CATG 6 cut(s) 60, 394, 559, 611, 734, 791
Hin6I GCGC 1 cut(s) 925
HinP1I GCGC 1 cut(s) 925
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 5 cut(s) 53, 106, 272, 846, 857
HpaII CCGG 2 cut(s) 21, 464
HphI GGTGA 3 cut(s) 103, 511, 707
Hpy166II GTNNAC 1 cut(s) 461
Hpy188I TCNGA 1 cut(s) 357
Hpy188III TCNNGA 6 cut(s) 187, 197, 642, 854, 952, 1049
Hpy8I GTNNAC 1 cut(s) 461
HpyAV CCTTC 2 cut(s) 705, 738
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4IV ACGT 4 cut(s) 172, 725, 891, 963
HpyF10VI GCNNNNNNNGC 5 cut(s) 76, 446, 781, 810, 813
HpyF3I CTNAG 2 cut(s) 305, 354
HpySE526I ACGT 4 cut(s) 172, 725, 891, 963
Hsp92II CATG 6 cut(s) 60, 394, 559, 611, 734, 791
HspAI GCGC 1 cut(s) 925
KflI GGGWCCC 2 cut(s) 17, 25
KpnI GGTACC 1 cut(s) 287
Ksp22I TGATCA 2 cut(s) 193, 367
Kzo9I GATC 6 cut(s) 193, 205, 297, 367, 474, 934
LmnI GCTCC 3 cut(s) 64, 221, 916
Lsp1109I GCAGC 6 cut(s) 54, 674, 685, 784, 825, 906
LweI GCATC 2 cut(s) 674, 898
MabI ACCWGGT 1 cut(s) 573
MaeI CTAG 6 cut(s) 71, 144, 654, 801, 942, 1074
MaeII ACGT 4 cut(s) 172, 725, 891, 963
MaeIII GTNAC 2 cut(s) 565, 944
MalI GATC 6 cut(s) 195, 207, 299, 369, 476, 936
MboI GATC 6 cut(s) 193, 205, 297, 367, 474, 934
MboII GAAGA 2 cut(s) 416, 1080
MflI RGATCY 1 cut(s) 474
MlsI TGGCCA 1 cut(s) 884
MluCI AATT 6 cut(s) 251, 267, 349, 632, 818, 1031
MluNI TGGCCA 1 cut(s) 884
MmeI TCCRAC 2 cut(s) 30, 813
MnlI CCTC 1 cut(s) 959
Mox20I TGGCCA 1 cut(s) 884
MscI TGGCCA 1 cut(s) 884
MseI TTAA 3 cut(s) 822, 1034, 1059
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 884
MspA1I CMGCKG 2 cut(s) 99, 676
MspI CCGG 2 cut(s) 21, 464
MspR9I CCNGG 6 cut(s) 21, 22, 115, 378, 465, 575
MvaI CCWGG 3 cut(s) 115, 378, 575
MvnI CGCG 2 cut(s) 539, 925
MwoI GCNNNNNNNGC 5 cut(s) 76, 446, 781, 810, 813
NciI CCSGG 3 cut(s) 21, 22, 465
NdeI CATATG 1 cut(s) 973
NdeII GATC 6 cut(s) 193, 205, 297, 367, 474, 934
NheI GCTAGC 1 cut(s) 800
NlaIII CATG 6 cut(s) 60, 394, 559, 611, 734, 791
NlaIV GGNNCC 6 cut(s) 18, 19, 26, 27, 285, 808
OliI CACNNNNGTG 1 cut(s) 41
PaeR7I CTCGAG 1 cut(s) 950
PfeI GAWTC 5 cut(s) 53, 106, 272, 846, 857
PkrI GCNGC 9 cut(s) 69, 664, 675, 799, 806, 815, 921, 924, 1019
PpuMI RGGWCCY 2 cut(s) 17, 25
PshBI ATTAAT 1 cut(s) 1034
Psp5II RGGWCCY 2 cut(s) 17, 25
Psp6I CCWGG 3 cut(s) 113, 376, 573
PspFI CCCAGC 1 cut(s) 99
PspGI CCWGG 3 cut(s) 113, 376, 573
PspN4I GGNNCC 6 cut(s) 18, 19, 26, 27, 285, 808
PspPI GGNCC 5 cut(s) 17, 25, 117, 461, 650
PspPPI RGGWCCY 2 cut(s) 17, 25
PstI CTGCAG 2 cut(s) 183, 675
PsuI RGATCY 1 cut(s) 474
PvuII CAGCTG 1 cut(s) 99
RsaI GTAC 3 cut(s) 123, 285, 536
RsaNI GTAC 3 cut(s) 122, 284, 535
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 3 cut(s) 822, 1034, 1059
SatI GCNGC 9 cut(s) 68, 663, 674, 798, 805, 814, 920, 923, 1018
Sau3AI GATC 6 cut(s) 193, 205, 297, 367, 474, 934
Sau96I GGNCC 5 cut(s) 17, 25, 117, 461, 650
ScrFI CCNGG 6 cut(s) 21, 22, 115, 378, 465, 575
SexAI ACCWGGT 1 cut(s) 573
SfaNI GCATC 2 cut(s) 674, 898
SfcI CTRYAG 2 cut(s) 179, 671
Sfr274I CTCGAG 1 cut(s) 950
SinI GGWCC 5 cut(s) 17, 25, 117, 461, 650
SlaI CTCGAG 1 cut(s) 950
SmaI CCCGGG 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 2 cut(s) 950, 992
SmoI CTYRAG 2 cut(s) 950, 992
Sse9I AATT 6 cut(s) 251, 267, 349, 632, 818, 1031
SsiI CCGC 5 cut(s) 400, 676, 804, 923, 1017
SspMI CTAG 6 cut(s) 71, 144, 654, 801, 942, 1074
StyD4I CCNGG 6 cut(s) 19, 20, 113, 376, 463, 573
TaaI ACNGT 1 cut(s) 121
TaiI ACGT 4 cut(s) 175, 728, 894, 966
TaqI TCGA 3 cut(s) 507, 709, 951
TasI AATT 6 cut(s) 251, 267, 349, 632, 818, 1031
TauI GCSGC 3 cut(s) 807, 925, 1020
TfiI GAWTC 5 cut(s) 53, 106, 272, 846, 857
Tru1I TTAA 3 cut(s) 822, 1034, 1059
Tru9I TTAA 3 cut(s) 822, 1034, 1059
TscAI CASTG 1 cut(s) 441
TseI GCWGC 6 cut(s) 67, 662, 673, 797, 813, 919
TspDTI ATGAA 5 cut(s) 45, 339, 360, 624, 625
TspMI CCCGGG 1 cut(s) 20
TspRI CASTG 1 cut(s) 441
VpaK11BI GGWCC 5 cut(s) 17, 25, 117, 461, 650
VspI ATTAAT 1 cut(s) 1034
XapI RAATTY 1 cut(s) 349
XhoI CTCGAG 1 cut(s) 950
XmaI CCCGGG 1 cut(s) 20
XspI CTAG 6 cut(s) 71, 144, 654, 801, 942, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.