RLG00000024077

Protein BUD31 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004887
Physical Location & Seq
Forward (+)
31518373 .. 31525985
7613 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024077

Sequence Viewer

Length: 903 bp
ATGTTGAGGACGATGAAGCCGGCTATGCAATTGAAGCCAAATCCCCGTACCCGAGAGATCGAGTCCCGCTCTTGCCTCTCTGCAACGCCGTCGACTCTGCTCACCGTAACCAGGTATAAAATGCCTAAGGTAAAGACTAATCGAGTCAACTACCCAGAAGGCTGGGAACTGATCGAGCCTACACTTCGCGAACTGCAAGCAAAGATGAGAGAAGCTGAGAATGATACTCACGATGGTAAGAGGAAATGTGAAACCTTATGGCCTATTTTCAAAATTGCGCATCAAAAGAGCCGTTATATTTTTGACCTTTACCATCGGAGAAAAGAAATTTCCAAGGAATTGTATGAGTTCTGCCTGGACCAAGGTTATGCAGACCGCAATCTAATTGCTAAGTGGAAGAAGCCTGGTTATGAACGTCTCTGCTGTTTGAGGTGCATGCAGCCTCGGGATCACAACTTTGCCACCACATGCGTCTGCCGAGTGCCCAAGCATCTGAGAGAGGAGAAGATTACCCTTTGTACCGTGTATAGAGACTGTACCCCTTTAACTGACGTTGAACCTTCTCAAGCTGGGACTTATAATTCTCCGGCAGCATCCTTCCTCACCATCTCGCTCGGCTCCGGCAGCACAGGACCTTCAGATGCGACAACTCCTTCGTCGATTCCGTTCTCCATTACCGTTCTCTGCCGGAGCCGACTCGAATGGAGACGAGCTGGAGTAGTTCAGCCTCGAATCTTCGAATCCAAACGGCAAACCCATCGCCGCGTCGTCCAACTTCGGCAACCTGACAATCTCGCCACGCGACGCGAGGAGGCTGAAGACGAAGCTTCAAAGGACGAGATCGAGAGCGCAAAGAGCTCTCAACGGCCTGAGATTCATCGGCACCGTCTTCCTCATCACTAG

Protein Analysis

301

Amino Acids

34.91

Weight (kDa)

9.48

Isoelectric Point (pI)

61.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BUD31 PF01125 41 - 173 2.8e-56 Pre-mRNA-splicing factor BUD31
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
Acc16I TGCGCA 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 882
AccBSI CCGCTC 1 cut(s) 69
AccI GTMKAC 1 cut(s) 92
AccII CGCG 4 cut(s) 189, 765, 802, 807
AciI CCGC 3 cut(s) 67, 376, 763
AclWI GGATC 1 cut(s) 456
AcsI RAATTY 1 cut(s) 327
AcuI CTGAAG 2 cut(s) 621, 837
AfaI GTAC 3 cut(s) 49, 520, 538
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 4 cut(s) 34, 271, 557, 831
AjnI CCWGG 3 cut(s) 110, 354, 403
AluBI AGCT 5 cut(s) 215, 569, 713, 827, 858
AluI AGCT 5 cut(s) 215, 569, 713, 827, 858
Alw21I GWGCWC 1 cut(s) 860
Alw26I GTCTC 3 cut(s) 422, 525, 700
AlwI GGATC 1 cut(s) 456
Ama87I CYCGRG 2 cut(s) 51, 444
AoxI GGCC 2 cut(s) 260, 866
ApeKI GCWGC 3 cut(s) 439, 590, 624
ApoI RAATTY 1 cut(s) 327
ArsI GACNNNNNNTTYG 2 cut(s) 637, 669
AspLEI GCGC 2 cut(s) 280, 851
AspS9I GGNCC 2 cut(s) 358, 632
AsuHPI GGTGA 2 cut(s) 94, 595
AsuII TTCGAA 1 cut(s) 738
AvaI CYCGRG 2 cut(s) 51, 444
AvaII GGWCC 2 cut(s) 358, 632
AxyI CCTNAGG 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 486
BanI GGYRCC 1 cut(s) 882
BanII GRGCYC 1 cut(s) 860
BbsI GAAGAC 2 cut(s) 825, 881
Bbv12I GWGCWC 1 cut(s) 860
BbvI GCAGC 3 cut(s) 451, 602, 636
BccI CCATC 4 cut(s) 227, 321, 614, 765
BceAI ACGGC 4 cut(s) 73, 276, 764, 881
BcgI CGANNNNNNTGC 4 cut(s) 72, 106, 740, 774
BciT130I CCWGG 3 cut(s) 112, 356, 405
BcoDI GTCTC 3 cut(s) 422, 525, 700
BfaI CTAG 1 cut(s) 901
BisI GCNGC 4 cut(s) 440, 591, 625, 763
BlsI GCNGC 4 cut(s) 441, 592, 626, 764
Bme1390I CCNGG 3 cut(s) 112, 356, 405
Bme18I GGWCC 2 cut(s) 358, 632
BmeT110I CYCGRG 2 cut(s) 51, 444
BmgT120I GGNCC 2 cut(s) 358, 632
BmiI GGNNCC 3 cut(s) 619, 692, 884
BmrFI CCNGG 3 cut(s) 112, 356, 405
BmsI GCATC 4 cut(s) 289, 499, 602, 631
BpiI GAAGAC 2 cut(s) 825, 881
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpmI CTGGAG 1 cut(s) 735
Bpu14I TTCGAA 1 cut(s) 738
BpuEI CTTGAG 1 cut(s) 549
BsaJI CCNNGG 3 cut(s) 333, 361, 443
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse118I RCCGGY 1 cut(s) 19
Bse21I CCTNAGG 1 cut(s) 126
BseBI CCWGG 3 cut(s) 112, 356, 405
BseDI CCNNGG 3 cut(s) 333, 361, 443
BseGI GGATG 1 cut(s) 593
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 3 cut(s) 207, 485, 861
BseRI GAGGAG 2 cut(s) 515, 824
BseSI GKGCMC 1 cut(s) 486
BseXI GCAGC 3 cut(s) 451, 602, 636
BseYI CCCAGC 2 cut(s) 162, 569
Bsh1236I CGCG 4 cut(s) 189, 765, 802, 807
BshFI GGCC 2 cut(s) 262, 868
BshNI GGYRCC 1 cut(s) 882
BsiHKAI GWGCWC 1 cut(s) 860
BsiHKCI CYCGRG 2 cut(s) 51, 444
BsiSI CCGG 4 cut(s) 20, 587, 621, 688
BslFI GGGAC 2 cut(s) 49, 586
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 3 cut(s) 422, 525, 700
BsmBI CGTCTC 2 cut(s) 422, 700
BsmFI GGGAC 2 cut(s) 49, 586
BsnI GGCC 2 cut(s) 262, 868
BsoBI CYCGRG 2 cut(s) 51, 444
Bsp119I TTCGAA 1 cut(s) 738
Bsp1286I GDGCHC 2 cut(s) 486, 860
Bsp143I GATC 4 cut(s) 57, 171, 448, 840
Bsp68I TCGCGA 1 cut(s) 189
BspACI CCGC 3 cut(s) 67, 376, 763
BspANI GGCC 2 cut(s) 262, 868
BspCNI CTCAG 3 cut(s) 208, 486, 862
BspFNI CGCG 4 cut(s) 189, 765, 802, 807
BspLI GGNNCC 3 cut(s) 619, 692, 884
BspPI GGATC 1 cut(s) 456
BspT104I TTCGAA 1 cut(s) 738
BspT107I GGYRCC 1 cut(s) 882
BsrBI CCGCTC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 19
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 3 cut(s) 333, 361, 443
BssMI GATC 4 cut(s) 57, 171, 448, 840
BssT1I CCWWGG 2 cut(s) 333, 361
Bst2UI CCWGG 3 cut(s) 112, 356, 405
Bst4CI ACNGT 5 cut(s) 106, 523, 536, 679, 887
BstBI TTCGAA 1 cut(s) 738
BstC8I GCNNGC 3 cut(s) 21, 198, 437
BstDEI CTNAG 5 cut(s) 126, 216, 390, 494, 870
BstF5I GGATG 1 cut(s) 593
BstFNI CGCG 4 cut(s) 189, 765, 802, 807
BstHHI GCGC 2 cut(s) 280, 851
BstKTI GATC 4 cut(s) 60, 174, 451, 843
BstMAI GTCTC 3 cut(s) 422, 525, 700
BstMBI GATC 4 cut(s) 57, 171, 448, 840
BstMWI GCNNNNNNNGC 4 cut(s) 25, 34, 624, 855
BstNI CCWGG 3 cut(s) 112, 356, 405
BstNSI RCATGY 2 cut(s) 439, 471
BstSCI CCNGG 3 cut(s) 110, 354, 403
BstSLI GKGCMC 1 cut(s) 486
BstUI CGCG 4 cut(s) 189, 765, 802, 807
BstV1I GCAGC 3 cut(s) 451, 602, 636
BstV2I GAAGAC 2 cut(s) 825, 881
BstXI CCANNNNNNTGG 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 126
BsuRI GGCC 2 cut(s) 262, 868
BtgZI GCGATG 1 cut(s) 743
BtsCI GGATG 1 cut(s) 593
BtuMI TCGCGA 1 cut(s) 189
Cac8I GCNNGC 3 cut(s) 21, 198, 437
CfoI GCGC 2 cut(s) 280, 851
Cfr10I RCCGGY 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 358, 632
CseI GACGC 3 cut(s) 460, 754, 813
CsiI ACCWGGT 1 cut(s) 110
Csp6I GTAC 3 cut(s) 48, 519, 537
CviAII CATG 2 cut(s) 436, 468
CviQI GTAC 3 cut(s) 48, 519, 537
DdeI CTNAG 5 cut(s) 126, 216, 390, 494, 870
DpnI GATC 4 cut(s) 59, 173, 450, 842
DpnII GATC 4 cut(s) 57, 171, 448, 840
Ecl136II GAGCTC 1 cut(s) 858
Eco130I CCWWGG 2 cut(s) 333, 361
Eco24I GRGCYC 1 cut(s) 860
Eco47I GGWCC 2 cut(s) 358, 632
Eco53kI GAGCTC 1 cut(s) 858
Eco57I CTGAAG 2 cut(s) 621, 837
Eco81I CCTNAGG 1 cut(s) 126
Eco88I CYCGRG 2 cut(s) 51, 444
EcoICRI GAGCTC 1 cut(s) 858
EcoO109I RGGNCCY 1 cut(s) 632
EcoRII CCWGG 3 cut(s) 110, 354, 403
EcoT14I CCWWGG 2 cut(s) 333, 361
EcoT38I GRGCYC 1 cut(s) 860
ErhI CCWWGG 2 cut(s) 333, 361
Esp3I CGTCTC 2 cut(s) 422, 700
FaeI CATG 2 cut(s) 439, 471
FaqI GGGAC 2 cut(s) 49, 586
FatI CATG 2 cut(s) 435, 467
FauI CCCGC 1 cut(s) 74
FblI GTMKAC 1 cut(s) 92
Fnu4HI GCNGC 4 cut(s) 440, 591, 625, 763
FokI GGATG 1 cut(s) 580
FriOI GRGCYC 1 cut(s) 860
Fsp4HI GCNGC 4 cut(s) 440, 591, 625, 763
FspBI CTAG 1 cut(s) 901
FspI TGCGCA 1 cut(s) 279
GlaI GCGC 2 cut(s) 279, 850
GluI GCNGC 4 cut(s) 440, 591, 625, 763
GsaI CCCAGC 2 cut(s) 166, 573
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 2 cut(s) 262, 868
HapII CCGG 4 cut(s) 20, 587, 621, 688
HgaI GACGC 3 cut(s) 460, 754, 813
HhaI GCGC 2 cut(s) 280, 851
Hin1II CATG 2 cut(s) 439, 471
Hin6I GCGC 2 cut(s) 278, 849
HinP1I GCGC 2 cut(s) 278, 849
HincII GTYRAC 2 cut(s) 93, 148
HindII GTYRAC 2 cut(s) 93, 148
HindIII AAGCTT 1 cut(s) 825
HinfI GANTC 8 cut(s) 62, 94, 144, 661, 696, 732, 740, 874
HpaII CCGG 4 cut(s) 20, 587, 621, 688
HphI GGTGA 2 cut(s) 94, 595
Hpy166II GTNNAC 2 cut(s) 93, 148
Hpy188I TCNGA 3 cut(s) 318, 495, 640
Hpy188III TCNNGA 4 cut(s) 188, 230, 446, 844
Hpy8I GTNNAC 2 cut(s) 93, 148
Hpy99I CGWCG 4 cut(s) 94, 661, 770, 807
HpyAV CCTTC 5 cut(s) 152, 570, 607, 645, 663
HpyCH4III ACNGT 5 cut(s) 106, 523, 536, 679, 887
HpyCH4IV ACGT 2 cut(s) 415, 552
HpyCH4V TGCA 6 cut(s) 28, 83, 196, 371, 435, 439
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 34, 624, 855
HpyF3I CTNAG 5 cut(s) 126, 216, 390, 494, 870
HpySE526I ACGT 2 cut(s) 415, 552
Hsp92II CATG 2 cut(s) 439, 471
HspAI GCGC 2 cut(s) 278, 849
KroI GCCGGC 1 cut(s) 19
KroNI GCCGGC 1 cut(s) 21
Kzo9I GATC 4 cut(s) 57, 171, 448, 840
LmnI GCTCC 2 cut(s) 623, 690
Lsp1109I GCAGC 3 cut(s) 451, 602, 636
LweI GCATC 4 cut(s) 289, 499, 602, 631
MabI ACCWGGT 1 cut(s) 110
MaeI CTAG 1 cut(s) 901
MaeII ACGT 2 cut(s) 415, 552
MaeIII GTNAC 1 cut(s) 106
MalI GATC 4 cut(s) 59, 173, 450, 842
MbiI CCGCTC 1 cut(s) 69
MboI GATC 4 cut(s) 57, 171, 448, 840
MboII GAAGA 5 cut(s) 409, 517, 727, 830, 881
MfeI CAATTG 1 cut(s) 29
MhlI GDGCHC 2 cut(s) 486, 860
MluCI AATT 6 cut(s) 29, 273, 327, 338, 384, 580
MlyI GAGTC 4 cut(s) 71, 88, 153, 690
MmeI TCCRAC 1 cut(s) 796
MroNI GCCGGC 1 cut(s) 19
MseI TTAA 1 cut(s) 545
MspI CCGG 4 cut(s) 20, 587, 621, 688
MspR9I CCNGG 3 cut(s) 112, 356, 405
MunI CAATTG 1 cut(s) 29
MvaI CCWGG 3 cut(s) 112, 356, 405
MvnI CGCG 4 cut(s) 189, 765, 802, 807
MwoI GCNNNNNNNGC 4 cut(s) 25, 34, 624, 855
NaeI GCCGGC 1 cut(s) 21
NdeII GATC 4 cut(s) 57, 171, 448, 840
NgoMIV GCCGGC 1 cut(s) 19
NlaIII CATG 2 cut(s) 439, 471
NlaIV GGNNCC 3 cut(s) 619, 692, 884
NmeAIII GCCGAG 2 cut(s) 503, 594
NruI TCGCGA 1 cut(s) 189
NsbI TGCGCA 1 cut(s) 279
NspI RCATGY 2 cut(s) 439, 471
NspV TTCGAA 1 cut(s) 738
PaeI GCATGC 1 cut(s) 439
PcsI WCGNNNNNNNCGW 1 cut(s) 662
PdiI GCCGGC 1 cut(s) 21
PfeI GAWTC 4 cut(s) 661, 732, 740, 874
PkrI GCNGC 4 cut(s) 441, 592, 626, 764
PleI GAGTC 4 cut(s) 70, 88, 152, 690
PpsI GAGTC 4 cut(s) 70, 88, 152, 690
PpuMI RGGWCCY 1 cut(s) 632
PsiI TTATAA 1 cut(s) 579
Psp124BI GAGCTC 1 cut(s) 860
Psp5II RGGWCCY 1 cut(s) 632
Psp6I CCWGG 3 cut(s) 110, 354, 403
PspFI CCCAGC 2 cut(s) 162, 569
PspGI CCWGG 3 cut(s) 110, 354, 403
PspN4I GGNNCC 3 cut(s) 619, 692, 884
PspPI GGNCC 2 cut(s) 358, 632
PspPPI RGGWCCY 1 cut(s) 632
RruI TCGCGA 1 cut(s) 189
RsaI GTAC 3 cut(s) 49, 520, 538
RsaNI GTAC 3 cut(s) 48, 519, 537
SacI GAGCTC 1 cut(s) 860
SalI GTCGAC 1 cut(s) 91
SaqAI TTAA 1 cut(s) 545
SatI GCNGC 4 cut(s) 440, 591, 625, 763
Sau3AI GATC 4 cut(s) 57, 171, 448, 840
Sau96I GGNCC 2 cut(s) 358, 632
SchI GAGTC 4 cut(s) 71, 88, 153, 690
ScrFI CCNGG 3 cut(s) 112, 356, 405
SduI GDGCHC 2 cut(s) 486, 860
SexAI ACCWGGT 1 cut(s) 110
SfaNI GCATC 4 cut(s) 289, 499, 602, 631
SfuI TTCGAA 1 cut(s) 738
SinI GGWCC 2 cut(s) 358, 632
SmlI CTYRAG 1 cut(s) 564
SmoI CTYRAG 1 cut(s) 564
SphI GCATGC 1 cut(s) 439
Sse9I AATT 6 cut(s) 29, 273, 327, 338, 384, 580
SsiI CCGC 3 cut(s) 67, 376, 763
SspMI CTAG 1 cut(s) 901
SstI GAGCTC 1 cut(s) 860
StyD4I CCNGG 3 cut(s) 110, 354, 403
StyI CCWWGG 2 cut(s) 333, 361
TaaI ACNGT 5 cut(s) 106, 523, 536, 679, 887
TaiI ACGT 2 cut(s) 418, 555
TaqI TCGA 9 cut(s) 60, 92, 142, 174, 659, 699, 730, 738, 843
TasI AATT 6 cut(s) 29, 273, 327, 338, 384, 580
TauI GCSGC 1 cut(s) 765
TfiI GAWTC 4 cut(s) 661, 732, 740, 874
Tru1I TTAA 1 cut(s) 545
Tru9I TTAA 1 cut(s) 545
TseI GCWGC 3 cut(s) 439, 590, 624
TspDTI ATGAA 3 cut(s) 29, 426, 866
TspGWI ACGGA 1 cut(s) 654
VpaK11BI GGWCC 2 cut(s) 358, 632
XapI RAATTY 1 cut(s) 327
XceI RCATGY 2 cut(s) 439, 471
XmiI GTMKAC 1 cut(s) 92
XspI CTAG 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.