Rroxscaffold_2G00124900

Transmembrane protein 87B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004887
Physical Location & Seq
Forward (+)
59452242 .. 59469144
16903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00124900.1

Sequence Viewer

Length: 1446 bp
ATGCCCCCATCCATATCTACCGCAACGAAATCTTCCGGGAAGTCGGCAATGCTTTCCTTCTCTCCGGCGGCAGCGAAGGCATCTCCGCCTCTCCCTCCTTCCGCTCTTTATCCGATTCGAGAATATCACCTTCGGAGGAGCGAGGCTGCTGTTGACAAGAAACAATGTAATGGGTTGATACGGGTTGTAAGCAAGCACTGTGATCCAAAGCTGAAGGAACTTGTAATGAGCGGGAAGACGGTATGGAAAAGCCCCGATGGTTATCTACACGGTCGAATGGCTCCACCGATGAAATTTTACGTGTTTATGGCACTTGCTTATGTCTTGATTGGTGTAATTTGGCTCACTCGGTATGTGAGGTTACGGAAGGATATTCTGCAACTTCAGCATTGCATCACGGCTGTCATTGCTCTTGGATTGTTTGAGATGATTCTTTGGGGAACCCAAAGGCTTCCTAGGGTCAAGATAAACCCCTTCATCGCATATGAAATGCTCCAATTGTGCATTGCAGCACAGTGCCTGGCCACTTTGATAGCTTCGGGAGCGTTTAGCTCTAAAAAAAACAACGGGAGTTTATTAGAGCGTTTTCACTCCACCGCTGCCCTCATCGTCATTCTCTCTCCTCCTCCTCGCATCCTCTTACTCCCCTTCATCGTTGCCGCTGCCGTCATTGCCATTCTCTTCAATTCTTGTGAGACAGCAGAGCTGCTTGTTGATGCTTCTCAATCATCCGAACGACCAGTATCTCCAACTCTCTTTGGAATTTTCTTGGAGGAGATTAACCATGCTGGAGCTGGTGGGTTGTGGGCTGAGCTTGTAAGCAACGAGAGGTACTTCTCAACTCTCCAGATAATGCAAAGTGAAAAAGAATTTAGACTCGACCGACCGAAGTACGTTTCACGAAACCTCCAACGCTCCGGCCAAAAGAATAGACACCAACAACAGCATTATGTAGCCTTTGATGAGAAAGTACGGAGCACGAATACGACGAGCAGGGAAACCATCACCACCATTGCATTCATCGAGGGAAAATCCTCTGCTGCTCCACGACGGATACCGCTCGCGCCGCCGGCCGATAGGTGGCTTCATTGGAGGCCTCTTGCGCCTCCTCTCCAACGTCCGCTCAAGCGTACGGGCTCTCCACGCCATGGCTGCACGTCGCTCTTCATTCAACGAAGTTTCGGCCCGATGTCGGTGAGTGATCCGATAGGCTACGTTTTCGGTGCCCTATTGCTTCGATTCGGGGACTTCCGAGACCGTGGGATCCCAGCGATACCCTCGCTCGGTGTGTTGTTGATCCTAGCCGGCGAGCGGTGGAAGAAATATTGGGTGCGGGCACGATGTGGTTTGCTTCTCGGGGATTCATTTGGACCGGGTTCGGGTGGTGTCATCGACATCGGGCTAGGGCCGAATCATGTTTCTGGCACGGTGGCGGCCGGACCTTAG

Protein Analysis

481

Amino Acids

53.02

Weight (kDa)

9.99

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GOST_TM PF06814 95 - 146 1.8e-13 GOST, seven transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1223
AccB7I CCANNNNNTGG 1 cut(s) 1148
AccBSI CCGCTC 5 cut(s) 104, 231, 1060, 1123, 1312
AccII CGCG 1 cut(s) 1064
AclWI GGATC 5 cut(s) 197, 1196, 1258, 1271, 1291
AcoI YGGCCR 4 cut(s) 522, 919, 1071, 1434
AcsI RAATTY 3 cut(s) 293, 762, 869
AcuI CTGAAG 2 cut(s) 233, 368
AdeI CACNNNGTG 1 cut(s) 1343
AfaI GTAC 4 cut(s) 833, 893, 972, 1132
AfiI CCNNNNNNNGG 6 cut(s) 1080, 1148, 1192, 1283, 1311, 1379
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 2 cut(s) 685, 1172
AjiI CACGTC 1 cut(s) 1158
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 6 cut(s) 211, 536, 552, 706, 794, 814
AluI AGCT 6 cut(s) 211, 536, 552, 706, 794, 814
Alw21I GWGCWC 1 cut(s) 980
Alw26I GTCTC 2 cut(s) 689, 1248
AlwI GGATC 5 cut(s) 197, 1196, 1258, 1271, 1291
AlwNI CAGNNNCTG 1 cut(s) 520
Ama87I CYCGRG 1 cut(s) 1355
AoxI GGCC 7 cut(s) 522, 919, 1071, 1094, 1183, 1406, 1434
ApeKI GCWGC 8 cut(s) 71, 146, 509, 599, 662, 706, 1040, 1152
ApoI RAATTY 3 cut(s) 293, 762, 869
AspA2I CCTAGG 1 cut(s) 455
AspLEI GCGC 2 cut(s) 1066, 1105
AspS9I GGNCC 4 cut(s) 1184, 1370, 1406, 1439
AsuC2I CCSGG 2 cut(s) 37, 1374
AsuHPI GGTGA 3 cut(s) 119, 997, 1207
AvaI CYCGRG 1 cut(s) 1355
AvaII GGWCC 2 cut(s) 1370, 1439
AvrII CCTAGG 1 cut(s) 455
BaeGI GKGCMC 2 cut(s) 1228, 1339
BalI TGGCCA 1 cut(s) 524
BamHI GGATCC 1 cut(s) 1263
BanI GGYRCC 1 cut(s) 1223
BanII GRGCYC 1 cut(s) 1139
BbsI GAAGAC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 980
BbvI GCAGC 8 cut(s) 83, 133, 521, 586, 649, 693, 1027, 1139
BccI CCATC 3 cut(s) 16, 251, 1010
BceAI ACGGC 2 cut(s) 414, 650
BciT130I CCWGG 1 cut(s) 521
BciVI GTATCC 1 cut(s) 1047
BcnI CCSGG 2 cut(s) 37, 1374
BcoDI GTCTC 2 cut(s) 689, 1248
BfaI CTAG 3 cut(s) 456, 1301, 1403
BfuI GTATCC 1 cut(s) 1047
BlnI CCTAGG 1 cut(s) 455
BlpI GCTNAGC 1 cut(s) 810
Bme1390I CCNGG 3 cut(s) 37, 521, 1374
Bme18I GGWCC 2 cut(s) 1370, 1439
BmeT110I CYCGRG 1 cut(s) 1355
BmgBI CACGTC 1 cut(s) 1158
BmgT120I GGNCC 4 cut(s) 1184, 1370, 1406, 1439
BmiI GGNNCC 4 cut(s) 282, 442, 1225, 1265
BmrFI CCNGG 3 cut(s) 37, 521, 1374
BmsI GCATC 4 cut(s) 89, 402, 642, 706
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 2 cut(s) 810, 830
Bpu1102I GCTNAGC 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 1109
BpuMI CCSGG 2 cut(s) 37, 1374
BsaAI YACGTR 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 1248
BsaJI CCNNGG 3 cut(s) 455, 1147, 1258
BsaXI ACNNNNNCTCC 4 cut(s) 891, 921, 1123, 1153
Bsc4I CCNNNNNNNGG 6 cut(s) 1080, 1148, 1192, 1283, 1311, 1379
Bse118I RCCGGY 2 cut(s) 1069, 1304
Bse1I ACTGG 1 cut(s) 740
Bse3DI GCAATG 6 cut(s) 54, 388, 405, 504, 669, 1011
Bse8I GATNNNNATC 1 cut(s) 261
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 3 cut(s) 455, 1147, 1258
BseGI GGATG 3 cut(s) 8, 633, 728
BseJI GATNNNNATC 1 cut(s) 261
BseLI CCNNNNNNNGG 6 cut(s) 1080, 1148, 1192, 1283, 1311, 1379
BseMI GCAATG 6 cut(s) 54, 388, 405, 504, 669, 1011
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 1 cut(s) 740
BseRI GAGGAG 6 cut(s) 151, 612, 615, 618, 788, 1098
BseSI GKGCMC 2 cut(s) 1228, 1339
BseX3I CGGCCG 2 cut(s) 1071, 1434
BseXI GCAGC 8 cut(s) 83, 133, 521, 586, 649, 693, 1027, 1139
BseYI CCCAGC 1 cut(s) 1267
BsgI GTGCAG 1 cut(s) 1138
Bsh1236I CGCG 1 cut(s) 1064
Bsh1285I CGRYCG 5 cut(s) 274, 883, 887, 1074, 1437
BshFI GGCC 7 cut(s) 524, 921, 1073, 1096, 1185, 1408, 1436
BshNI GGYRCC 1 cut(s) 1223
BsiEI CGRYCG 5 cut(s) 274, 883, 887, 1074, 1437
BsiHKAI GWGCWC 1 cut(s) 980
BsiHKCI CYCGRG 1 cut(s) 1355
BsiSI CCGG 7 cut(s) 36, 65, 918, 1070, 1305, 1373, 1437
BsiWI CGTACG 1 cut(s) 1130
BslFI GGGAC 1 cut(s) 1259
BslI CCNNNNNNNGG 6 cut(s) 1080, 1148, 1192, 1283, 1311, 1379
BsmAI GTCTC 2 cut(s) 689, 1248
BsmFI GGGAC 1 cut(s) 1259
BsmI GAATGC 1 cut(s) 1016
BsnI GGCC 7 cut(s) 524, 921, 1073, 1096, 1185, 1408, 1436
Bso31I GGTCTC 1 cut(s) 1248
BsoBI CYCGRG 1 cut(s) 1355
Bsp1286I GDGCHC 4 cut(s) 980, 1139, 1228, 1339
Bsp143I GATC 4 cut(s) 202, 1201, 1263, 1296
Bsp1720I GCTNAGC 1 cut(s) 810
Bsp19I CCATGG 1 cut(s) 1147
BspANI GGCC 7 cut(s) 524, 921, 1073, 1096, 1185, 1408, 1436
BspCNI CTCAG 1 cut(s) 802
BspFNI CGCG 1 cut(s) 1064
BspLI GGNNCC 4 cut(s) 282, 442, 1225, 1265
BspPI GGATC 5 cut(s) 197, 1196, 1258, 1271, 1291
BspQI GCTCTTC 1 cut(s) 1169
BspT107I GGYRCC 1 cut(s) 1223
BspTNI GGTCTC 1 cut(s) 1248
BsrBI CCGCTC 5 cut(s) 104, 231, 1060, 1123, 1312
BsrDI GCAATG 6 cut(s) 54, 388, 405, 504, 669, 1011
BsrFI RCCGGY 2 cut(s) 1069, 1304
BsrI ACTGG 1 cut(s) 740
BssAI RCCGGY 2 cut(s) 1069, 1304
BssECI CCNNGG 3 cut(s) 455, 1147, 1258
BssMI GATC 4 cut(s) 202, 1201, 1263, 1296
BssT1I CCWWGG 2 cut(s) 455, 1147
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 6 cut(s) 200, 241, 272, 516, 1259, 1429
Bst6I CTCTTC 2 cut(s) 686, 1169
BstBAI YACGTR 1 cut(s) 301
BstC8I GCNNGC 6 cut(s) 194, 1062, 1071, 1306, 1310, 1335
BstDEI CTNAG 2 cut(s) 810, 1443
BstDSI CCRYGG 2 cut(s) 1147, 1258
BstF5I GGATG 3 cut(s) 8, 633, 728
BstFNI CGCG 1 cut(s) 1064
BstHHI GCGC 2 cut(s) 1066, 1105
BstKTI GATC 4 cut(s) 205, 1204, 1266, 1299
BstMAI GTCTC 2 cut(s) 689, 1248
BstMBI GATC 4 cut(s) 202, 1201, 1263, 1296
BstMCI CGRYCG 5 cut(s) 274, 883, 887, 1074, 1437
BstNI CCWGG 1 cut(s) 521
BstSCI CCNGG 3 cut(s) 35, 519, 1372
BstSLI GKGCMC 2 cut(s) 1228, 1339
BstUI CGCG 1 cut(s) 1064
BstV1I GCAGC 8 cut(s) 83, 133, 521, 586, 649, 693, 1027, 1139
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 1263
BstYI RGATCY 1 cut(s) 1263
BstZI CGGCCG 2 cut(s) 1071, 1434
BsuI GTATCC 1 cut(s) 1047
BsuRI GGCC 7 cut(s) 524, 921, 1073, 1096, 1185, 1408, 1436
BtgI CCRYGG 2 cut(s) 1147, 1258
BtgZI GCGATG 1 cut(s) 463
BtrI CACGTC 1 cut(s) 1158
BtsCI GGATG 3 cut(s) 8, 633, 728
BtsIMutI CAGTG 2 cut(s) 196, 521
Cac8I GCNNGC 6 cut(s) 194, 1062, 1071, 1306, 1310, 1335
CaiI CAGNNNCTG 1 cut(s) 520
CfoI GCGC 2 cut(s) 1066, 1105
Cfr10I RCCGGY 2 cut(s) 1069, 1304
Cfr13I GGNCC 4 cut(s) 1184, 1370, 1406, 1439
Csp6I GTAC 4 cut(s) 832, 892, 971, 1131
CviAII CATG 3 cut(s) 785, 1148, 1415
CviQI GTAC 4 cut(s) 832, 892, 971, 1131
DdeI CTNAG 2 cut(s) 810, 1443
DpnI GATC 4 cut(s) 204, 1203, 1265, 1298
DpnII GATC 4 cut(s) 202, 1201, 1263, 1296
DraIII CACNNNGTG 1 cut(s) 1343
EaeI YGGCCR 4 cut(s) 522, 919, 1071, 1434
EagI CGGCCG 2 cut(s) 1071, 1434
Eam1104I CTCTTC 2 cut(s) 686, 1169
EarI CTCTTC 2 cut(s) 686, 1169
EciI GGCGGA 1 cut(s) 75
EclXI CGGCCG 2 cut(s) 1071, 1434
Eco130I CCWWGG 2 cut(s) 455, 1147
Eco147I AGGCCT 1 cut(s) 1096
Eco24I GRGCYC 1 cut(s) 1139
Eco31I GGTCTC 1 cut(s) 1248
Eco47I GGWCC 2 cut(s) 1370, 1439
Eco52I CGGCCG 2 cut(s) 1071, 1434
Eco57I CTGAAG 2 cut(s) 233, 368
Eco88I CYCGRG 1 cut(s) 1355
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 2 cut(s) 455, 1147
EcoT38I GRGCYC 1 cut(s) 1139
ErhI CCWWGG 2 cut(s) 455, 1147
FaeI CATG 3 cut(s) 788, 1151, 1418
FaqI GGGAC 1 cut(s) 1259
FatI CATG 3 cut(s) 784, 1147, 1414
FauI CCCGC 2 cut(s) 224, 1326
FauNDI CATATG 1 cut(s) 484
FokI GGATG 2 cut(s) 620, 715
FriOI GRGCYC 1 cut(s) 1139
FspBI CTAG 3 cut(s) 456, 1301, 1403
GlaI GCGC 2 cut(s) 1065, 1104
GsaI CCCAGC 1 cut(s) 1271
GsuI CTGGAG 2 cut(s) 810, 830
HaeIII GGCC 7 cut(s) 524, 921, 1073, 1096, 1185, 1408, 1436
HapII CCGG 7 cut(s) 36, 65, 918, 1070, 1305, 1373, 1437
HhaI GCGC 2 cut(s) 1066, 1105
Hin1II CATG 3 cut(s) 788, 1151, 1418
Hin6I GCGC 2 cut(s) 1064, 1103
HinP1I GCGC 2 cut(s) 1064, 1103
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 6 cut(s) 115, 430, 876, 1239, 1361, 1411
HpaII CCGG 7 cut(s) 36, 65, 918, 1070, 1305, 1373, 1437
HphI GGTGA 3 cut(s) 119, 997, 1207
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 5 cut(s) 114, 135, 733, 1206, 1253
Hpy188III TCNNGA 6 cut(s) 119, 325, 463, 540, 847, 900
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 3 cut(s) 991, 1053, 1162
HpyAV CCTTC 8 cut(s) 67, 70, 108, 140, 208, 361, 484, 658
HpyCH4III ACNGT 6 cut(s) 200, 241, 272, 516, 1259, 1429
HpyCH4IV ACGT 5 cut(s) 300, 894, 1117, 1157, 1215
HpyCH4V TGCA 7 cut(s) 379, 393, 504, 509, 856, 1016, 1155
HpyF3I CTNAG 2 cut(s) 810, 1443
HpySE526I ACGT 5 cut(s) 300, 894, 1117, 1157, 1215
Hsp92II CATG 3 cut(s) 788, 1151, 1418
HspAI GCGC 2 cut(s) 1064, 1103
KroI GCCGGC 2 cut(s) 1069, 1304
KroNI GCCGGC 2 cut(s) 1071, 1306
Kzo9I GATC 4 cut(s) 202, 1201, 1263, 1296
LguI GCTCTTC 1 cut(s) 1169
LmnI GCTCC 8 cut(s) 138, 286, 498, 542, 791, 920, 975, 1048
Lsp1109I GCAGC 8 cut(s) 83, 133, 521, 586, 649, 693, 1027, 1139
LweI GCATC 4 cut(s) 89, 402, 642, 706
MaeI CTAG 3 cut(s) 456, 1301, 1403
MaeII ACGT 5 cut(s) 300, 894, 1117, 1157, 1215
MaeIII GTNAC 1 cut(s) 360
MalI GATC 4 cut(s) 204, 1203, 1265, 1298
MbiI CCGCTC 5 cut(s) 104, 231, 1060, 1123, 1312
MboI GATC 4 cut(s) 202, 1201, 1263, 1296
MboII GAAGA 5 cut(s) 24, 247, 673, 1156, 1330
MfeI CAATTG 1 cut(s) 497
MflI RGATCY 1 cut(s) 1263
MhlI GDGCHC 4 cut(s) 980, 1139, 1228, 1339
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 6 cut(s) 293, 336, 497, 685, 762, 869
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 1 cut(s) 870
MmeI TCCRAC 3 cut(s) 773, 934, 1138
Mox20I TGGCCA 1 cut(s) 524
MroNI GCCGGC 2 cut(s) 1069, 1304
MscI TGGCCA 1 cut(s) 524
MseI TTAA 1 cut(s) 780
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 2 cut(s) 599, 662
MspI CCGG 7 cut(s) 36, 65, 918, 1070, 1305, 1373, 1437
MspR9I CCNGG 3 cut(s) 37, 521, 1374
MunI CAATTG 1 cut(s) 497
Mva1269I GAATGC 1 cut(s) 1016
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 1 cut(s) 1064
NaeI GCCGGC 2 cut(s) 1071, 1306
NciI CCSGG 2 cut(s) 37, 1374
NcoI CCATGG 1 cut(s) 1147
NdeI CATATG 1 cut(s) 484
NdeII GATC 4 cut(s) 202, 1201, 1263, 1296
NgoMIV GCCGGC 2 cut(s) 1069, 1304
NlaIII CATG 3 cut(s) 788, 1151, 1418
NlaIV GGNNCC 4 cut(s) 282, 442, 1225, 1265
PceI AGGCCT 1 cut(s) 1096
PciSI GCTCTTC 1 cut(s) 1169
PcsI WCGNNNNNNNCGW 1 cut(s) 986
PctI GAATGC 1 cut(s) 1016
PdiI GCCGGC 2 cut(s) 1071, 1306
PfeI GAWTC 5 cut(s) 115, 430, 1239, 1361, 1411
Pfl23II CGTACG 1 cut(s) 1130
PflMI CCANNNNNTGG 1 cut(s) 1148
PfoI TCCNGGA 1 cut(s) 35
PleI GAGTC 1 cut(s) 870
PpsI GAGTC 1 cut(s) 870
Ppu21I YACGTR 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 519
PspFI CCCAGC 1 cut(s) 1267
PspGI CCWGG 1 cut(s) 519
PspLI CGTACG 1 cut(s) 1130
PspN4I GGNNCC 4 cut(s) 282, 442, 1225, 1265
PspPI GGNCC 4 cut(s) 1184, 1370, 1406, 1439
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 1 cut(s) 1263
RsaI GTAC 4 cut(s) 833, 893, 972, 1132
RsaNI GTAC 4 cut(s) 832, 892, 971, 1131
SapI GCTCTTC 1 cut(s) 1169
SaqAI TTAA 1 cut(s) 780
Sau3AI GATC 4 cut(s) 202, 1201, 1263, 1296
Sau96I GGNCC 4 cut(s) 1184, 1370, 1406, 1439
SchI GAGTC 1 cut(s) 870
ScrFI CCNGG 3 cut(s) 37, 521, 1374
SduI GDGCHC 4 cut(s) 980, 1139, 1228, 1339
SfaNI GCATC 4 cut(s) 89, 402, 642, 706
SinI GGWCC 2 cut(s) 1370, 1439
SmlI CTYRAG 1 cut(s) 1124
SmoI CTYRAG 1 cut(s) 1124
Sse9I AATT 6 cut(s) 293, 336, 497, 685, 762, 869
SseBI AGGCCT 1 cut(s) 1096
SspI AATATT 1 cut(s) 1325
SspMI CTAG 3 cut(s) 456, 1301, 1403
StuI AGGCCT 1 cut(s) 1096
StyD4I CCNGG 3 cut(s) 35, 519, 1372
StyI CCWWGG 2 cut(s) 455, 1147
TaaI ACNGT 6 cut(s) 200, 241, 272, 516, 1259, 1429
TaiI ACGT 5 cut(s) 303, 897, 1120, 1160, 1218
TaqI TCGA 6 cut(s) 118, 274, 879, 1023, 1237, 1392
TaqII GACCGA 2 cut(s) 897, 901
TasI AATT 6 cut(s) 293, 336, 497, 685, 762, 869
TauI GCSGC 4 cut(s) 71, 662, 1069, 1436
TfiI GAWTC 5 cut(s) 115, 430, 1239, 1361, 1411
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TscAI CASTG 2 cut(s) 203, 521
TseI GCWGC 8 cut(s) 71, 146, 509, 599, 662, 706, 1040, 1152
TspDTI ATGAA 8 cut(s) 305, 466, 501, 640, 1009, 1076, 1156, 1353
TspGWI ACGGA 3 cut(s) 379, 988, 1066
TspRI CASTG 2 cut(s) 203, 521
Van91I CCANNNNNTGG 1 cut(s) 1148
VpaK11BI GGWCC 2 cut(s) 1370, 1439
XapI RAATTY 3 cut(s) 293, 762, 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 791
XmaJI CCTAGG 1 cut(s) 455
XspI CTAG 3 cut(s) 456, 1301, 1403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.