RLG00000025221

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003283
Physical Location & Seq
Reverse (-)
43066575 .. 43067657
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025221

Sequence Viewer

Length: 486 bp
ATGCTATTGTTATGGTGTCATGGTTGTTCTGCTGTGTTGTGGTCTGGTCTTTTGTCGGTGCTTTTTGGTTATCAACGTGATGGTGAATCACCCTTACACGTGGTCTGGCGGTTTTGGGTCGCGGTGTTCGAACGGGTGAAAACTGTTCATCGTAATGTTGGTGACTGGGTTGTATCGATCGGTATAGGTTTGATTGTTACTCATTGTAACTCGCGGCTGACTACTCATCGGAATCTCCGCCGGCAATTGAAGGTCGGTCAGACGGTGCAGCCGCAGAAGACGAAGAAGAAGAAGAGCATCGAGACCAAGGCTACCGACTCCACTGCTGTGACCATCGACCAGACGACGTCGTCCACCACGATCGACAGGCTCAGCTCCGGCAACCACCTCAGCATACTCTGCGGGGTCCACCCCATCGATGACCGGAATGCTGACATGAACCGCCACCAGGACATTTCTGAGCTTCTAAGTCTCGACTTCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

162

Amino Acids

18.17

Weight (kDa)

9.05

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 349
AatII GACGTC 1 cut(s) 350
AccII CGCG 2 cut(s) 122, 214
AciI CCGC 7 cut(s) 109, 122, 214, 238, 272, 402, 442
AcvI CACGTG 1 cut(s) 100
AcyI GRCGYC 1 cut(s) 347
AfiI CCNNNNNNNGG 1 cut(s) 448
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 1 cut(s) 447
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 2 cut(s) 375, 463
AluI AGCT 2 cut(s) 375, 463
Alw26I GTCTC 2 cut(s) 296, 476
ApeKI GCWGC 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 406
AsuHPI GGTGA 4 cut(s) 81, 95, 148, 173
AsuII TTCGAA 1 cut(s) 129
AvaII GGWCC 1 cut(s) 406
BbrPI CACGTG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 284
BbvCI CCTCAGC 1 cut(s) 389
BbvI GCAGC 1 cut(s) 280
BccI CCATC 3 cut(s) 74, 341, 422
BcgI CGANNNNNNTGC 2 cut(s) 305, 339
BciT130I CCWGG 1 cut(s) 449
BcoDI GTCTC 2 cut(s) 296, 476
BisI GCNGC 3 cut(s) 215, 269, 272
BlpI GCTNAGC 1 cut(s) 371
BlsI GCNGC 3 cut(s) 216, 270, 273
Bme1390I CCNGG 1 cut(s) 449
Bme18I GGWCC 1 cut(s) 406
BmgT120I GGNCC 1 cut(s) 406
BmiI GGNNCC 1 cut(s) 407
BmrFI CCNGG 1 cut(s) 449
BmrI ACTGGG 1 cut(s) 175
BmsI GCATC 1 cut(s) 306
BmuI ACTGGG 1 cut(s) 175
BpiI GAAGAC 1 cut(s) 284
Bpu10I CCTNAGC 1 cut(s) 389
Bpu1102I GCTNAGC 1 cut(s) 371
Bpu14I TTCGAA 1 cut(s) 129
Bsa29I ATCGAT 2 cut(s) 176, 417
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 347
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 306
BsaWI WCCGGW 1 cut(s) 423
Bsc4I CCNNNNNNNGG 1 cut(s) 448
Bse118I RCCGGY 1 cut(s) 240
Bse1I ACTGG 1 cut(s) 170
BseBI CCWGG 1 cut(s) 449
BseCI ATCGAT 2 cut(s) 176, 417
BseDI CCNNGG 1 cut(s) 306
BseLI CCNNNNNNNGG 1 cut(s) 448
BseMII CTCAG 3 cut(s) 385, 403, 450
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 1 cut(s) 280
BsgI GTGCAG 1 cut(s) 287
Bsh1236I CGCG 2 cut(s) 122, 214
Bsh1285I CGRYCG 2 cut(s) 180, 363
BshVI ATCGAT 2 cut(s) 176, 417
BsiEI CGRYCG 2 cut(s) 180, 363
BsiSI CCGG 3 cut(s) 241, 378, 424
BslI CCNNNNNNNGG 1 cut(s) 448
BsmAI GTCTC 2 cut(s) 296, 476
BsmI GAATGC 1 cut(s) 433
Bso31I GGTCTC 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 129
Bsp143I GATC 2 cut(s) 177, 360
Bsp1720I GCTNAGC 1 cut(s) 371
BspACI CCGC 7 cut(s) 109, 122, 214, 238, 272, 402, 442
BspCNI CTCAG 3 cut(s) 384, 402, 451
BspDI ATCGAT 2 cut(s) 176, 417
BspFNI CGCG 2 cut(s) 122, 214
BspLI GGNNCC 1 cut(s) 407
BspQI GCTCTTC 1 cut(s) 287
BspT104I TTCGAA 1 cut(s) 129
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 240
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 306
BssMI GATC 2 cut(s) 177, 360
BssNI GRCGYC 1 cut(s) 347
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 2 cut(s) 145, 265
Bst6I CTCTTC 1 cut(s) 287
BstACI GRCGYC 1 cut(s) 347
BstAPI GCANNNNNTGC 1 cut(s) 399
BstBAI YACGTR 1 cut(s) 100
BstBI TTCGAA 1 cut(s) 129
BstC8I GCNNGC 1 cut(s) 242
BstDEI CTNAG 4 cut(s) 371, 389, 459, 467
BstFNI CGCG 2 cut(s) 122, 214
BstKTI GATC 2 cut(s) 180, 363
BstMAI GTCTC 2 cut(s) 296, 476
BstMBI GATC 2 cut(s) 177, 360
BstMCI CGRYCG 2 cut(s) 180, 363
BstMWI GCNNNNNNNGC 1 cut(s) 399
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 447
BstUI CGCG 2 cut(s) 122, 214
BstV1I GCAGC 1 cut(s) 280
BstV2I GAAGAC 1 cut(s) 284
Bsu15I ATCGAT 2 cut(s) 176, 417
BsuTUI ATCGAT 2 cut(s) 176, 417
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 1 cut(s) 321
Cac8I GCNNGC 1 cut(s) 242
Cfr10I RCCGGY 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 406
ClaI ATCGAT 2 cut(s) 176, 417
CviAII CATG 2 cut(s) 20, 436
CviJI RGCY 6 cut(s) 217, 271, 311, 370, 375, 463
CviKI_1 RGCY 6 cut(s) 217, 271, 311, 370, 375, 463
DdeI CTNAG 4 cut(s) 371, 389, 459, 467
DpnI GATC 2 cut(s) 179, 362
DpnII GATC 2 cut(s) 177, 360
DrdI GACNNNNNNGTC 1 cut(s) 349
DseDI GACNNNNNNGTC 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
EciI GGCGGA 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 406
Eco72I CACGTG 1 cut(s) 100
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 23, 439
FaiI YATR 5 cut(s) 13, 21, 185, 395, 437
FatI CATG 2 cut(s) 19, 435
FauI CCCGC 1 cut(s) 395
Fnu4HI GCNGC 3 cut(s) 215, 269, 272
Fsp4HI GCNGC 3 cut(s) 215, 269, 272
GluI GCNGC 3 cut(s) 215, 269, 272
HapII CCGG 3 cut(s) 241, 378, 424
Hin1I GRCGYC 1 cut(s) 347
Hin1II CATG 2 cut(s) 23, 439
HinfI GANTC 3 cut(s) 86, 232, 317
HpaII CCGG 3 cut(s) 241, 378, 424
HphI GGTGA 4 cut(s) 81, 95, 148, 173
Hpy166II GTNNAC 2 cut(s) 354, 409
Hpy188I TCNGA 3 cut(s) 231, 261, 460
Hpy188III TCNNGA 2 cut(s) 301, 473
Hpy8I GTNNAC 2 cut(s) 354, 409
Hpy99I CGWCG 2 cut(s) 349, 352
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 145, 265
HpyCH4IV ACGT 3 cut(s) 76, 99, 347
HpyCH4V TGCA 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 1 cut(s) 399
HpyF3I CTNAG 4 cut(s) 371, 389, 459, 467
HpySE526I ACGT 3 cut(s) 76, 99, 347
Hsp92I GRCGYC 1 cut(s) 347
Hsp92II CATG 2 cut(s) 23, 439
KroI GCCGGC 1 cut(s) 240
KroNI GCCGGC 1 cut(s) 242
Kzo9I GATC 2 cut(s) 177, 360
LguI GCTCTTC 1 cut(s) 287
LmnI GCTCC 1 cut(s) 380
Lsp1109I GCAGC 1 cut(s) 280
LweI GCATC 1 cut(s) 306
MaeII ACGT 3 cut(s) 76, 99, 347
MaeIII GTNAC 4 cut(s) 161, 196, 206, 328
MalI GATC 2 cut(s) 179, 362
MboI GATC 2 cut(s) 177, 360
MboII GAAGA 5 cut(s) 289, 295, 298, 301, 304
MfeI CAATTG 1 cut(s) 245
MluCI AATT 1 cut(s) 245
MlyI GAGTC 1 cut(s) 311
MnlI CCTC 1 cut(s) 398
MroNI GCCGGC 1 cut(s) 240
MslI CAYNNNNRTG 2 cut(s) 153, 326
MspI CCGG 3 cut(s) 241, 378, 424
MspR9I CCNGG 1 cut(s) 449
MunI CAATTG 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 433
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 2 cut(s) 122, 214
MwoI GCNNNNNNNGC 1 cut(s) 399
NaeI GCCGGC 1 cut(s) 242
NdeII GATC 2 cut(s) 177, 360
NgoMIV GCCGGC 1 cut(s) 240
NlaIII CATG 2 cut(s) 23, 439
NlaIV GGNNCC 1 cut(s) 407
NmuCI GTSAC 2 cut(s) 161, 328
NspV TTCGAA 1 cut(s) 129
OliI CACNNNNGTG 1 cut(s) 326
PciSI GCTCTTC 1 cut(s) 287
PcsI WCGNNNNNNNCGW 2 cut(s) 126, 356
PctI GAATGC 1 cut(s) 433
PdiI GCCGGC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 86, 232
PflFI GACNNNGTC 2 cut(s) 346, 349
PkrI GCNGC 3 cut(s) 216, 270, 273
Ple19I CGATCG 2 cut(s) 180, 363
PleI GAGTC 1 cut(s) 311
PmaCI CACGTG 1 cut(s) 100
PmlI CACGTG 1 cut(s) 100
PpsI GAGTC 1 cut(s) 311
Ppu21I YACGTR 1 cut(s) 100
Psp6I CCWGG 1 cut(s) 447
PspCI CACGTG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 1 cut(s) 407
PspPI GGNCC 1 cut(s) 406
PsyI GACNNNGTC 2 cut(s) 346, 349
PvuI CGATCG 2 cut(s) 180, 363
RseI CAYNNNNRTG 2 cut(s) 153, 326
SapI GCTCTTC 1 cut(s) 287
SatI GCNGC 3 cut(s) 215, 269, 272
Sau3AI GATC 2 cut(s) 177, 360
Sau96I GGNCC 1 cut(s) 406
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 1 cut(s) 449
SetI ASST 8 cut(s) 79, 102, 190, 255, 350, 377, 390, 465
SfaNI GCATC 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 129
SinI GGWCC 1 cut(s) 406
SmiMI CAYNNNNRTG 2 cut(s) 153, 326
Sse9I AATT 1 cut(s) 245
SsiI CCGC 7 cut(s) 109, 122, 214, 238, 272, 402, 442
StyD4I CCNGG 1 cut(s) 447
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 145, 265
TaiI ACGT 3 cut(s) 79, 102, 350
TaqI TCGA 8 cut(s) 129, 176, 300, 336, 363, 417, 474, 480
TaqII GACCGA 1 cut(s) 245
TasI AATT 1 cut(s) 245
TauI GCSGC 2 cut(s) 217, 274
TfiI GAWTC 2 cut(s) 86, 232
TscAI CASTG 1 cut(s) 328
TseFI GTSAC 2 cut(s) 161, 328
TseI GCWGC 1 cut(s) 268
Tsp45I GTSAC 2 cut(s) 161, 328
TspDTI ATGAA 2 cut(s) 137, 452
TspRI CASTG 1 cut(s) 328
Tth111I GACNNNGTC 2 cut(s) 346, 349
VpaK11BI GGWCC 1 cut(s) 406
ZraI GACGTC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.