Rh4AG189500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0003283
Physical Location & Seq
Forward (+)
46972798 .. 46983750
10953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG189500.1

Sequence Viewer

Length: 279 bp
ATGATCAAAAGAAAGATTACTCTGAAGACACAAATGAGTTATATATGGTTGAAGCTAAGGATAAGGATTGAATACTCCCACAAATCGCCGCCGGAAATCTCCGTGAACCCACAATCCGATTTGGGGATCCCTACTTCACAGGCAGACTTGCTTAGTTGCTTGTGCGTTTGGATGGAGCCTGGAGTTGCTGCTGTGAATCTGGATTTCTGGGAGGCAGACATGGAAGGCTCGGCACTGGAGCTGCTAGCACTGCCATTCATTGTCACTCTAAGCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

92

Amino Acids

10.36

Weight (kDa)

5.21

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 89
AclWI GGATC 2 cut(s) 121, 134
AcuI CTGAAG 1 cut(s) 44
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 2 cut(s) 52, 71
AjnI CCWGG 1 cut(s) 178
AloI GAACNNNNNNTCC 2 cut(s) 98, 130
AluBI AGCT 2 cut(s) 55, 241
AluI AGCT 2 cut(s) 55, 241
AlwI GGATC 2 cut(s) 121, 134
ApeKI GCWGC 2 cut(s) 188, 241
AsuNHI GCTAGC 1 cut(s) 244
BamHI GGATCC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 175, 228
BccI CCATC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 180
BclI TGATCA 1 cut(s) 3
BfaI CTAG 1 cut(s) 245
BisI GCNGC 3 cut(s) 89, 189, 242
BlsI GCNGC 3 cut(s) 90, 190, 243
Bme1390I CCNGG 1 cut(s) 180
BmiI GGNNCC 2 cut(s) 128, 177
BmrFI CCNGG 1 cut(s) 180
BmtI GCTAGC 1 cut(s) 248
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 2 cut(s) 201, 257
Bpu10I CCTNAGC 1 cut(s) 56
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 180
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 240
BseXI GCAGC 2 cut(s) 175, 228
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 1 cut(s) 123
Bsp143I GATC 2 cut(s) 3, 126
BspACI CCGC 1 cut(s) 89
BspLI GGNNCC 2 cut(s) 128, 177
BspOI GCTAGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 121, 134
BsrI ACTGG 1 cut(s) 240
BssMI GATC 2 cut(s) 3, 126
Bst2UI CCWGG 1 cut(s) 180
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 3 cut(s) 56, 152, 269
BstF5I GGATG 1 cut(s) 177
BstKTI GATC 2 cut(s) 6, 129
BstMBI GATC 2 cut(s) 3, 126
BstMWI GCNNNNNNNGC 1 cut(s) 250
BstNI CCWGG 1 cut(s) 180
BstSCI CCNGG 1 cut(s) 178
BstV1I GCAGC 2 cut(s) 175, 228
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BtsCI GGATG 1 cut(s) 177
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 2 cut(s) 233, 248
Cac8I GCNNGC 1 cut(s) 246
CviAII CATG 2 cut(s) 220, 276
CviJI RGCY 5 cut(s) 55, 178, 228, 241, 273
CviKI_1 RGCY 5 cut(s) 55, 178, 228, 241, 273
DdeI CTNAG 3 cut(s) 56, 152, 269
DpnI GATC 2 cut(s) 5, 128
DpnII GATC 2 cut(s) 3, 126
Eco57I CTGAAG 1 cut(s) 44
EcoRII CCWGG 1 cut(s) 178
FaeI CATG 2 cut(s) 223, 279
FaiI YATR 5 cut(s) 42, 44, 46, 221, 277
FatI CATG 2 cut(s) 219, 275
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 89, 189, 242
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 3 cut(s) 89, 189, 242
FspBI CTAG 1 cut(s) 245
GluI GCNGC 3 cut(s) 89, 189, 242
GsuI CTGGAG 2 cut(s) 201, 257
HapII CCGG 1 cut(s) 92
Hin1II CATG 2 cut(s) 223, 279
HinfI GANTC 1 cut(s) 196
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 24, 118
Hpy188III TCNNGA 1 cut(s) 200
Hpy8I GTNNAC 1 cut(s) 106
HpyAV CCTTC 1 cut(s) 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 250
HpyF3I CTNAG 3 cut(s) 56, 152, 269
Hsp92II CATG 2 cut(s) 223, 279
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 126
LmnI GCTCC 2 cut(s) 175, 238
LpnPI CCDG 7 cut(s) 105, 125, 165, 185, 192, 193, 221
Lsp1109I GCAGC 2 cut(s) 175, 228
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 1 cut(s) 262
MalI GATC 2 cut(s) 5, 128
MboI GATC 2 cut(s) 3, 126
MboII GAAGA 1 cut(s) 37
MflI RGATCY 1 cut(s) 126
MnlI CCTC 1 cut(s) 205
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 180
MvaI CCWGG 1 cut(s) 180
MwoI GCNNNNNNNGC 1 cut(s) 250
NdeII GATC 2 cut(s) 3, 126
NheI GCTAGC 1 cut(s) 244
NlaIII CATG 2 cut(s) 223, 279
NlaIV GGNNCC 2 cut(s) 128, 177
NmeAIII GCCGAG 1 cut(s) 209
NmuCI GTSAC 1 cut(s) 262
PfeI GAWTC 1 cut(s) 196
PkrI GCNGC 3 cut(s) 90, 190, 243
Psp6I CCWGG 1 cut(s) 178
PspGI CCWGG 1 cut(s) 178
PspN4I GGNNCC 2 cut(s) 128, 177
PsuI RGATCY 1 cut(s) 126
SatI GCNGC 3 cut(s) 89, 189, 242
Sau3AI GATC 2 cut(s) 3, 126
ScrFI CCNGG 1 cut(s) 180
SetI ASST 2 cut(s) 57, 243
SsiI CCGC 1 cut(s) 89
SspMI CTAG 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 178
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 1 cut(s) 196
TscAI CASTG 2 cut(s) 240, 255
TseFI GTSAC 1 cut(s) 262
TseI GCWGC 2 cut(s) 188, 241
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 247
TspGWI ACGGA 1 cut(s) 91
TspRI CASTG 2 cut(s) 240, 255
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.