Rmu_sc0002267.1_g000016

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002267.1
OG0003283
Physical Location & Seq
Forward (+)
53849 .. 55838
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002267.1_g000016.1.cds

Sequence Viewer

Length: 654 bp
atggcttcttcgatcgacgacgccatgggtcttgctcgacggcgatggggattgcgaggcatggctcgaggatggcgtttccgtgatgatgcaggttgctccagcatggctcggcagtttggatcacggagatggtgcagcggtgcatatggtggctacgcggtgatgtggttggccaaaacgtggccggactgtggtgcgcgacgggtctgggaggagtggtggactggtccaaaccaagaagatgggcctgatgtttctgggcatttatcggtgctttctggttatcagcgtgatggtgaatcacccttacatgtggtctggttgttttgggaggcaatgtccaaacgggtggaaacagttcatcttaatgctggtggcggggttgtatcgttatacataggtccaaatacctcaagcataccagttgcaggcatcgctggaaagctgtggacattcactcaattcggaacaatttatgtcaccgatgacaccatgacccaaggaccgaacataacatcttccgtagtaggaagggcccaaggcataacggtggcatcggcactggacggaagcaatacccttgtcttggtgtctcgtgttcacaaacatgaagtacaatggcaggactctagagatacaaggaaacagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

217

Amino Acids

23.97

Weight (kDa)

8.88

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 83
AccB7I CCANNNNNTGG 1 cut(s) 183
AccII CGCG 2 cut(s) 161, 202
AciI CCGC 3 cut(s) 141, 161, 381
AclWI GGATC 1 cut(s) 130
AcoI YGGCCR 2 cut(s) 174, 185
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 4 cut(s) 183, 194, 431, 589
AflIII ACRYGT 1 cut(s) 313
AluBI AGCT 1 cut(s) 448
AluI AGCT 1 cut(s) 448
Alw26I GTCTC 1 cut(s) 600
AlwI GGATC 1 cut(s) 130
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 4 cut(s) 174, 185, 248, 537
ApaI GGGCCC 1 cut(s) 541
ApeKI GCWGC 1 cut(s) 138
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 6 cut(s) 230, 248, 404, 506, 537, 538
AsuHPI GGTGA 4 cut(s) 175, 297, 311, 475
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 3 cut(s) 230, 404, 506
BaeGI GKGCMC 1 cut(s) 541
BalI TGGCCA 1 cut(s) 176
BanII GRGCYC 1 cut(s) 541
BauI CACGAG 1 cut(s) 597
BbvI GCAGC 1 cut(s) 150
BccI CCATC 5 cut(s) 39, 66, 126, 239, 290
BceAI ACGGC 1 cut(s) 56
BcoDI GTCTC 1 cut(s) 600
BfaI CTAG 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 83
BisI GCNGC 1 cut(s) 139
BlsI GCNGC 1 cut(s) 140
Bme18I GGWCC 3 cut(s) 230, 404, 506
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 6 cut(s) 230, 248, 404, 506, 537, 538
BmiI GGNNCC 1 cut(s) 539
BmsI GCATC 3 cut(s) 79, 444, 566
BpmI CTGGAG 1 cut(s) 85
BpuEI CTTGAG 1 cut(s) 400
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 3 cut(s) 24, 502, 541
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 326, 356
Bsc4I CCNNNNNNNGG 4 cut(s) 183, 194, 431, 589
Bse1I ACTGG 3 cut(s) 232, 425, 570
Bse3DI GCAATG 1 cut(s) 345
BseDI CCNNGG 3 cut(s) 24, 502, 541
BseGI GGATG 1 cut(s) 77
BseLI CCNNNNNNNGG 4 cut(s) 183, 194, 431, 589
BseMI GCAATG 1 cut(s) 345
BseNI ACTGG 3 cut(s) 232, 425, 570
BseRI GAGGAG 1 cut(s) 230
BseSI GKGCMC 1 cut(s) 541
BseXI GCAGC 1 cut(s) 150
BsgI GTGCAG 1 cut(s) 157
Bsh1236I CGCG 2 cut(s) 161, 202
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 4 cut(s) 176, 187, 250, 539
BsiEI CGRYCG 1 cut(s) 15
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 1 cut(s) 188
BslI CCNNNNNNNGG 4 cut(s) 183, 194, 431, 589
BsmAI GTCTC 1 cut(s) 600
BsnI GGCC 4 cut(s) 176, 187, 250, 539
BsoBI CYCGRG 1 cut(s) 66
Bsp120I GGGCCC 1 cut(s) 537
Bsp1286I GDGCHC 1 cut(s) 541
Bsp143I GATC 2 cut(s) 12, 122
Bsp19I CCATGG 1 cut(s) 24
BspACI CCGC 3 cut(s) 141, 161, 381
BspANI GGCC 4 cut(s) 176, 187, 250, 539
BspFNI CGCG 2 cut(s) 161, 202
BspLI GGNNCC 1 cut(s) 539
BspMI ACCTGC 1 cut(s) 83
BspPI GGATC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 3 cut(s) 232, 425, 570
BssECI CCNNGG 3 cut(s) 24, 502, 541
BssMI GATC 2 cut(s) 12, 122
BssNI GRCGYC 1 cut(s) 21
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 3 cut(s) 24, 502, 541
Bst2BI CACGAG 1 cut(s) 597
Bst4CI ACNGT 4 cut(s) 194, 361, 553, 651
BstACI GRCGYC 1 cut(s) 21
BstC8I GCNNGC 1 cut(s) 433
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 77
BstFNI CGCG 2 cut(s) 161, 202
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 2 cut(s) 15, 125
BstMAI GTCTC 1 cut(s) 600
BstMBI GATC 2 cut(s) 12, 122
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNSI RCATGY 1 cut(s) 317
BstSLI GKGCMC 1 cut(s) 541
BstUI CGCG 2 cut(s) 161, 202
BstV1I GCAGC 1 cut(s) 150
BstXI CCANNNNNNTGG 2 cut(s) 245, 352
BsuRI GGCC 4 cut(s) 176, 187, 250, 539
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 2 cut(s) 58, 421
BtsCI GGATG 1 cut(s) 77
BtsIMutI CAGTG 1 cut(s) 563
BveI ACCTGC 1 cut(s) 83
Cac8I GCNNGC 1 cut(s) 433
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 6 cut(s) 230, 248, 404, 506, 537, 538
CseI GACGC 1 cut(s) 29
Csp6I GTAC 1 cut(s) 617
CviAII CATG 6 cut(s) 25, 61, 106, 314, 496, 611
CviJI RGCY 9 cut(s) 5, 65, 110, 156, 176, 187, 250, 448, 539
CviKI_1 RGCY 9 cut(s) 5, 65, 110, 156, 176, 187, 250, 448, 539
CviQI GTAC 1 cut(s) 617
DpnI GATC 2 cut(s) 14, 124
DpnII GATC 2 cut(s) 12, 122
EaeI YGGCCR 2 cut(s) 174, 185
Eco130I CCWWGG 3 cut(s) 24, 502, 541
Eco24I GRGCYC 1 cut(s) 541
Eco47I GGWCC 3 cut(s) 230, 404, 506
Eco88I CYCGRG 1 cut(s) 66
EcoO109I RGGNCCY 1 cut(s) 537
EcoT14I CCWWGG 3 cut(s) 24, 502, 541
EcoT38I GRGCYC 1 cut(s) 541
ErhI CCWWGG 3 cut(s) 24, 502, 541
FaeI CATG 6 cut(s) 28, 64, 109, 317, 499, 614
FatI CATG 6 cut(s) 24, 60, 105, 313, 495, 610
FauI CCCGC 1 cut(s) 374
FauNDI CATATG 1 cut(s) 148
Fnu4HI GCNGC 1 cut(s) 139
FokI GGATG 1 cut(s) 84
FriOI GRGCYC 1 cut(s) 541
Fsp4HI GCNGC 1 cut(s) 139
FspBI CTAG 1 cut(s) 633
GlaI GCGC 1 cut(s) 201
GluI GCNGC 1 cut(s) 139
GsuI CTGGAG 1 cut(s) 85
HaeIII GGCC 4 cut(s) 176, 187, 250, 539
HapII CCGG 1 cut(s) 188
HgaI GACGC 1 cut(s) 29
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 6 cut(s) 28, 64, 109, 317, 499, 614
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 2 cut(s) 302, 629
HpaII CCGG 1 cut(s) 188
HphI GGTGA 4 cut(s) 175, 297, 311, 475
Hpy166II GTNNAC 3 cut(s) 225, 453, 604
Hpy188I TCNGA 1 cut(s) 470
Hpy188III TCNNGA 1 cut(s) 633
Hpy8I GTNNAC 3 cut(s) 225, 453, 604
Hpy99I CGWCG 4 cut(s) 20, 23, 42, 207
HpyAV CCTTC 1 cut(s) 528
HpyCH4III ACNGT 4 cut(s) 194, 361, 553, 651
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 4 cut(s) 92, 138, 146, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpySE526I ACGT 1 cut(s) 182
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 6 cut(s) 28, 64, 109, 317, 499, 614
HspAI GCGC 1 cut(s) 200
Kzo9I GATC 2 cut(s) 12, 122
LmnI GCTCC 1 cut(s) 104
Lsp1109I GCAGC 1 cut(s) 150
LweI GCATC 3 cut(s) 79, 444, 566
MaeI CTAG 1 cut(s) 633
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 1 cut(s) 481
MalI GATC 2 cut(s) 14, 124
MboI GATC 2 cut(s) 12, 122
MboII GAAGA 2 cut(s) 254, 513
MhlI GDGCHC 1 cut(s) 541
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 2 cut(s) 464, 474
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 623
MnlI CCTC 5 cut(s) 50, 62, 208, 328, 424
Mox20I TGGCCA 1 cut(s) 176
MroXI GAANNNNTTC 1 cut(s) 360
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 4 cut(s) 130, 369, 551, 609
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 1 cut(s) 188
MvnI CGCG 2 cut(s) 161, 202
MwoI GCNNNNNNNGC 1 cut(s) 437
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 148
NdeII GATC 2 cut(s) 12, 122
NlaIII CATG 6 cut(s) 28, 64, 109, 317, 499, 614
NlaIV GGNNCC 1 cut(s) 539
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 481
NspI RCATGY 1 cut(s) 317
PaeR7I CTCGAG 1 cut(s) 66
PciI ACATGT 1 cut(s) 313
PcsI WCGNNNNNNNCGW 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 1 cut(s) 302
PflMI CCANNNNNTGG 1 cut(s) 183
PkrI GCNGC 1 cut(s) 140
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
PscI ACATGT 1 cut(s) 313
PspN4I GGNNCC 1 cut(s) 539
PspOMI GGGCCC 1 cut(s) 537
PspPI GGNCC 6 cut(s) 230, 248, 404, 506, 537, 538
PspXI VCTCGAGB 1 cut(s) 66
PvuI CGATCG 1 cut(s) 15
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
RseI CAYNNNNRTG 4 cut(s) 130, 369, 551, 609
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 1 cut(s) 139
Sau3AI GATC 2 cut(s) 12, 122
Sau96I GGNCC 6 cut(s) 230, 248, 404, 506, 537, 538
SchI GAGTC 1 cut(s) 623
SduI GDGCHC 1 cut(s) 541
SetI ASST 5 cut(s) 97, 185, 406, 416, 450
SfaNI GCATC 3 cut(s) 79, 444, 566
Sfr274I CTCGAG 1 cut(s) 66
SinI GGWCC 3 cut(s) 230, 404, 506
SlaI CTCGAG 1 cut(s) 66
SmiMI CAYNNNNRTG 4 cut(s) 130, 369, 551, 609
SmlI CTYRAG 2 cut(s) 66, 415
SmoI CTYRAG 2 cut(s) 66, 415
Sse9I AATT 2 cut(s) 464, 474
SsiI CCGC 3 cut(s) 141, 161, 381
SspMI CTAG 1 cut(s) 633
StyI CCWWGG 3 cut(s) 24, 502, 541
TaaI ACNGT 4 cut(s) 194, 361, 553, 651
TaiI ACGT 1 cut(s) 185
TaqI TCGA 4 cut(s) 11, 15, 37, 67
TaqII GACCGA 1 cut(s) 523
TasI AATT 2 cut(s) 464, 474
TatI WGTACW 1 cut(s) 616
TfiI GAWTC 1 cut(s) 302
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 1 cut(s) 570
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 1 cut(s) 138
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 353, 627
TspGWI ACGGA 4 cut(s) 71, 142, 514, 585
TspRI CASTG 1 cut(s) 570
Van91I CCANNNNNTGG 1 cut(s) 183
VpaK11BI GGWCC 3 cut(s) 230, 404, 506
XbaI TCTAGA 1 cut(s) 632
XceI RCATGY 1 cut(s) 317
XhoI CTCGAG 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.