RLG00000031416

disease resistance protein At4g27220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002553
Physical Location & Seq
Reverse (-)
4959768 .. 4962466
2699 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031416

Sequence Viewer

Length: 1341 bp
ATGGCAGAAACAGTTGCAGTTGTTTCCAGCGGCTTCGGCTGTCTAACTGTAGTTGTAACTCTGGCATCACCCTGTTTACGTGATCCCCTATCAGAAGAATACAACATTTTCAGGAAAAGAAAAGAAAACCTTGCTACTTTACAAAGTGAAATTCAAAACCTGGAACAGCAAATTAGTGAAGTGGAACGACAATCCAAGGAGAGCAGGTCTTTACGAGTATCCGACTGGTTGGACAAGGCACGAAAATTTATCAAGGATGTTGCTGACTCAAATTTTAAAGGCAGACTACTTGAGAATTGCTTTGGTCTACCCTACTGTAGGTCACAGTATAAGGCTGGAAAAGAAATTGCAGAAAGAATTACACAGTTGACTGCACTTGAGAAGGAAGAAGAAATTGCCGAGAATAAGTTACAAGTGGATCTTAATAACTCACCGGGGAGTAGAGCAGACACATTGGGGAGGGCAGTGAAGAAGTTGAAATTTCTGATCATTTTGGATGATATGTGGACAGATGACATCCACATTGATAAGAAGTTTGAGATTAAACTTCTCTCAGGTACAGATGCAGAAGCATTGTTCGAGACTGAAGCCGGCATTAAGTTATCACAACTGGGGGATGATACTCGAGCCACAGCAAAGAACATGATAGAGGAACGTGATGGTTTTCCTTTGGCAATTATCTTGCTGGCACGAGCCTTGAGAGAATCAAAGACCGACGATGCTGAGGATGTTGATGGTGAATGGAAAAAAGCTTTGACTTGGTTGAAAGAATCTACAGCCCCCTTGGAAAGCATGAATGAGAGGGCATTTCCGCGTCTGAAATATAGCTATGATATGTTGAAGAAAGATGCTCAGCAGTGTTTCCTTTACTGTGCATTATACCCAGCTGGTCATGACATTGAAAAAAAAGAGTTGGTGGAGTATTGGTTTTGGGAGGGTTTATTGGATGGCAGAAAGAGAAGTGGGCAGATCGAAGATATGAAACGAGCAAAAGAGATAATAGATGAGCTGAATGGAGCTCACCTTCACGAAATAGAAAGTCAGGAAGGTGGCAAAGAATACATCAAGATGAAAAACTTAGTTAGGCACATGGCAGTCCATCTTACCAAGATGAGCCCTGATCAGTTTCTCATCAAAGCAGGGGAGAAGCTTTCTGACTTCCCATCACAAGGCGAGTACTGGTCTGAAGTAGAGAGAGCCTCATTGATGAGAAATGAGTTTAAAGCACTTCGAGAAGAACCCAAGTTTCCCAAGCGATCTACATTACTTCTGCAGCACAATCCCATCACTCACTTGCATGACAATTTCTTTCGCAGTATGCCTAAACATCAAAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

447

Amino Acids

51.3

Weight (kDa)

5.84

Isoelectric Point (pI)

52.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 293 - 365 8.1e-07 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 307
AccII CGCG 1 cut(s) 814
AciI CCGC 2 cut(s) 30, 812
AclWI GGATC 2 cut(s) 77, 426
AcsI RAATTY 4 cut(s) 150, 245, 271, 479
AcuI CTGAAG 2 cut(s) 606, 1206
AfaI GTAC 2 cut(s) 559, 1178
AfiI CCNNNNNNNGG 2 cut(s) 318, 1169
AgsI TTSAA 5 cut(s) 155, 478, 766, 841, 902
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 6 cut(s) 752, 828, 887, 1009, 1019, 1150
AluI AGCT 6 cut(s) 752, 828, 887, 1009, 1019, 1150
Alw21I GWGCWC 1 cut(s) 1021
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 2 cut(s) 77, 426
Ama87I CYCGRG 1 cut(s) 624
ApeKI GCWGC 1 cut(s) 1273
ApoI RAATTY 4 cut(s) 150, 245, 271, 479
AsuC2I CCSGG 1 cut(s) 435
AsuHPI GGTGA 4 cut(s) 60, 423, 749, 1013
AvaI CYCGRG 1 cut(s) 624
BanII GRGCYC 2 cut(s) 1021, 1118
BauI CACGAG 1 cut(s) 690
Bbv12I GWGCWC 1 cut(s) 1021
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 1 cut(s) 1285
BccI CCATC 6 cut(s) 653, 728, 941, 1107, 1171, 1292
BciT130I CCWGG 1 cut(s) 161
BciVI GTATCC 1 cut(s) 229
BclI TGATCA 2 cut(s) 486, 1120
BcnI CCSGG 1 cut(s) 435
BcoDI GTCTC 1 cut(s) 575
BfmI CTRYAG 4 cut(s) 48, 316, 774, 1271
BfuAI ACCTGC 1 cut(s) 195
BfuI GTATCC 1 cut(s) 229
BisI GCNGC 2 cut(s) 31, 1274
BlpI GCTNAGC 1 cut(s) 852
BlsI GCNGC 2 cut(s) 32, 1275
BmcAI AGTACT 1 cut(s) 1178
Bme1390I CCNGG 2 cut(s) 161, 435
BmeT110I CYCGRG 1 cut(s) 624
BmrFI CCNGG 2 cut(s) 161, 435
BmrI ACTGGG 1 cut(s) 620
BmsI GCATC 4 cut(s) 74, 553, 709, 838
BmuI ACTGGG 1 cut(s) 620
BplI GAGNNNNNCTC 2 cut(s) 1184, 1216
Bpu10I CCTNAGC 1 cut(s) 723
Bpu1102I GCTNAGC 1 cut(s) 852
BpuEI CTTGAG 3 cut(s) 311, 398, 718
BpuMI CCSGG 1 cut(s) 435
BsaAI YACGTR 1 cut(s) 80
BsaJI CCNNGG 3 cut(s) 195, 434, 783
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 911, 941
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 1169
Bse118I RCCGGY 1 cut(s) 590
Bse1I ACTGG 3 cut(s) 230, 615, 1184
BseBI CCWGG 1 cut(s) 161
BseDI CCNNGG 3 cut(s) 195, 434, 783
BseGI GGATG 6 cut(s) 262, 502, 516, 622, 733, 952
BseLI CCNNNNNNNGG 2 cut(s) 318, 1169
BseMII CTCAG 3 cut(s) 567, 714, 866
BseNI ACTGG 3 cut(s) 230, 615, 1184
BseXI GCAGC 1 cut(s) 1285
BseYI CCCAGC 1 cut(s) 883
BsgI GTGCAG 1 cut(s) 357
Bsh1236I CGCG 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 1021
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 2 cut(s) 434, 591
BslI CCNNNNNNNGG 2 cut(s) 318, 1169
BsmAI GTCTC 1 cut(s) 575
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 2 cut(s) 1021, 1118
Bsp143I GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
Bsp1720I GCTNAGC 1 cut(s) 852
BspACI CCGC 2 cut(s) 30, 812
BspCNI CTCAG 3 cut(s) 566, 715, 865
BspFNI CGCG 1 cut(s) 814
BspHI TCATGA 1 cut(s) 892
BspMAI CTGCAG 1 cut(s) 1275
BspMI ACCTGC 1 cut(s) 195
BspPI GGATC 2 cut(s) 77, 426
BsrFI RCCGGY 1 cut(s) 590
BsrI ACTGG 3 cut(s) 230, 615, 1184
BssAI RCCGGY 1 cut(s) 590
BssECI CCNNGG 3 cut(s) 195, 434, 783
BssMI GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
BssSI CACGAG 1 cut(s) 690
BssT1I CCWWGG 2 cut(s) 195, 783
Bst2BI CACGAG 1 cut(s) 690
Bst2UI CCWGG 1 cut(s) 161
Bst4CI ACNGT 6 cut(s) 13, 49, 317, 327, 366, 872
BstBAI YACGTR 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 592, 687
BstDEI CTNAG 4 cut(s) 553, 723, 852, 1078
BstENI CCTNNNNNAGG 1 cut(s) 316
BstF5I GGATG 6 cut(s) 262, 502, 516, 622, 733, 952
BstFNI CGCG 1 cut(s) 814
BstKTI GATC 6 cut(s) 85, 421, 489, 972, 1123, 1259
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
BstMWI GCNNNNNNNGC 1 cut(s) 36
BstNI CCWGG 1 cut(s) 161
BstSCI CCNGG 2 cut(s) 159, 433
BstSFI CTRYAG 4 cut(s) 48, 316, 774, 1271
BstUI CGCG 1 cut(s) 814
BstV1I GCAGC 1 cut(s) 1285
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuI GTATCC 1 cut(s) 229
BtsCI GGATG 6 cut(s) 262, 502, 516, 622, 733, 952
BtsI GCAGTG 2 cut(s) 471, 863
BtsIMutI CAGTG 2 cut(s) 471, 863
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 592, 687
CciI TCATGA 1 cut(s) 892
Cfr10I RCCGGY 1 cut(s) 590
CseI GACGC 1 cut(s) 803
Csp6I GTAC 2 cut(s) 558, 1177
CviAII CATG 5 cut(s) 643, 793, 893, 1090, 1298
CviQI GTAC 2 cut(s) 558, 1177
DdeI CTNAG 4 cut(s) 553, 723, 852, 1078
DpnI GATC 6 cut(s) 84, 420, 488, 971, 1122, 1258
DpnII GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
DraI TTTAAA 2 cut(s) 277, 1222
Ecl136II GAGCTC 1 cut(s) 1019
Eco130I CCWWGG 2 cut(s) 195, 783
Eco24I GRGCYC 2 cut(s) 1021, 1118
Eco53kI GAGCTC 1 cut(s) 1019
Eco57I CTGAAG 2 cut(s) 606, 1206
Eco88I CYCGRG 1 cut(s) 624
EcoICRI GAGCTC 1 cut(s) 1019
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 159
EcoT14I CCWWGG 2 cut(s) 195, 783
EcoT38I GRGCYC 2 cut(s) 1021, 1118
ErhI CCWWGG 2 cut(s) 195, 783
FaeI CATG 5 cut(s) 646, 796, 896, 1093, 1301
FalI AAGNNNNNCTT 4 cut(s) 114, 146, 405, 437
FatI CATG 5 cut(s) 642, 792, 892, 1089, 1297
FbaI TGATCA 2 cut(s) 486, 1120
FblI GTMKAC 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 31, 1274
FokI GGATG 6 cut(s) 269, 503, 509, 629, 740, 959
FriOI GRGCYC 2 cut(s) 1021, 1118
Fsp4HI GCNGC 2 cut(s) 31, 1274
GluI GCNGC 2 cut(s) 31, 1274
GsaI CCCAGC 1 cut(s) 887
HapII CCGG 2 cut(s) 434, 591
HgaI GACGC 1 cut(s) 803
Hin1II CATG 5 cut(s) 646, 796, 896, 1093, 1301
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HindIII AAGCTT 2 cut(s) 750, 1148
HinfI GANTC 3 cut(s) 266, 704, 770
HpaII CCGG 2 cut(s) 434, 591
HphI GGTGA 4 cut(s) 60, 423, 749, 1013
Hpy166II GTNNAC 4 cut(s) 77, 308, 369, 507
Hpy188I TCNGA 6 cut(s) 94, 223, 486, 819, 1156, 1186
Hpy188III TCNNGA 8 cut(s) 112, 580, 893, 1028, 1043, 1066, 1232, 1338
Hpy8I GTNNAC 4 cut(s) 77, 308, 369, 507
Hpy99I CGWCG 1 cut(s) 719
HpyAV CCTTC 3 cut(s) 376, 1034, 1040
HpyCH4III ACNGT 6 cut(s) 13, 49, 317, 327, 366, 872
HpyCH4IV ACGT 2 cut(s) 79, 655
HpyCH4V TGCA 7 cut(s) 17, 350, 374, 566, 875, 1273, 1297
HpyF10VI GCNNNNNNNGC 1 cut(s) 36
HpyF3I CTNAG 4 cut(s) 553, 723, 852, 1078
HpySE526I ACGT 2 cut(s) 79, 655
Hsp92II CATG 5 cut(s) 646, 796, 896, 1093, 1301
KroI GCCGGC 1 cut(s) 590
KroNI GCCGGC 1 cut(s) 592
Ksp22I TGATCA 2 cut(s) 486, 1120
Kzo9I GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
LmnI GCTCC 1 cut(s) 1016
Lsp1109I GCAGC 1 cut(s) 1285
LweI GCATC 4 cut(s) 74, 553, 709, 838
MaeII ACGT 2 cut(s) 79, 655
MaeIII GTNAC 3 cut(s) 55, 321, 408
MalI GATC 6 cut(s) 84, 420, 488, 971, 1122, 1258
MboI GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
MboII GAAGA 7 cut(s) 107, 398, 401, 481, 853, 986, 1247
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 2 cut(s) 1021, 1118
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 2 cut(s) 210, 246
MnlI CCTC 6 cut(s) 453, 643, 718, 795, 928, 1210
MroNI GCCGGC 1 cut(s) 590
MseI TTAA 5 cut(s) 276, 423, 543, 597, 1221
MslI CAYNNNNRTG 2 cut(s) 1067, 1296
MspA1I CMGCKG 2 cut(s) 30, 887
MspI CCGG 2 cut(s) 434, 591
MspR9I CCNGG 2 cut(s) 161, 435
MvaI CCWGG 1 cut(s) 161
MvnI CGCG 1 cut(s) 814
MwoI GCNNNNNNNGC 1 cut(s) 36
NaeI GCCGGC 1 cut(s) 592
NciI CCSGG 1 cut(s) 435
NdeII GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
NgoMIV GCCGGC 1 cut(s) 590
NlaIII CATG 5 cut(s) 646, 796, 896, 1093, 1301
NmeAIII GCCGAG 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 624
PagI TCATGA 1 cut(s) 892
PdiI GCCGGC 1 cut(s) 592
PfeI GAWTC 2 cut(s) 704, 770
PkrI GCNGC 2 cut(s) 32, 1275
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 80
Psp124BI GAGCTC 1 cut(s) 1021
Psp6I CCWGG 1 cut(s) 159
PspFI CCCAGC 1 cut(s) 883
PspGI CCWGG 1 cut(s) 159
PspXI VCTCGAGB 1 cut(s) 624
PstI CTGCAG 1 cut(s) 1275
PsuI RGATCY 1 cut(s) 418
PvuII CAGCTG 1 cut(s) 887
RsaI GTAC 2 cut(s) 559, 1178
RsaNI GTAC 2 cut(s) 558, 1177
RseI CAYNNNNRTG 2 cut(s) 1067, 1296
SacI GAGCTC 1 cut(s) 1021
SaqAI TTAA 5 cut(s) 276, 423, 543, 597, 1221
SatI GCNGC 2 cut(s) 31, 1274
Sau3AI GATC 6 cut(s) 82, 418, 486, 969, 1120, 1256
ScaI AGTACT 1 cut(s) 1178
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 161, 435
SduI GDGCHC 2 cut(s) 1021, 1118
SfaNI GCATC 4 cut(s) 74, 553, 709, 838
SfcI CTRYAG 4 cut(s) 48, 316, 774, 1271
Sfr274I CTCGAG 1 cut(s) 624
SlaI CTCGAG 1 cut(s) 624
SmiMI CAYNNNNRTG 2 cut(s) 1067, 1296
SmlI CTYRAG 4 cut(s) 290, 377, 624, 697
SmoI CTYRAG 4 cut(s) 290, 377, 624, 697
SsiI CCGC 2 cut(s) 30, 812
SstI GAGCTC 1 cut(s) 1021
StyD4I CCNGG 2 cut(s) 159, 433
StyI CCWWGG 2 cut(s) 195, 783
TaaI ACNGT 6 cut(s) 13, 49, 317, 327, 366, 872
TaiI ACGT 2 cut(s) 82, 658
TaqI TCGA 4 cut(s) 579, 625, 972, 1231
TaqII GACCGA 1 cut(s) 728
TatI WGTACW 1 cut(s) 1176
TauI GCSGC 1 cut(s) 33
TfiI GAWTC 2 cut(s) 704, 770
Tru1I TTAA 5 cut(s) 276, 423, 543, 597, 1221
Tru9I TTAA 5 cut(s) 276, 423, 543, 597, 1221
TscAI CASTG 2 cut(s) 471, 863
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 1 cut(s) 1273
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 3 cut(s) 809, 995, 1085
TspRI CASTG 2 cut(s) 471, 863
XagI CCTNNNNNAGG 1 cut(s) 316
XapI RAATTY 4 cut(s) 150, 245, 271, 479
XhoI CTCGAG 1 cut(s) 624
XmiI GTMKAC 1 cut(s) 307
ZrmI AGTACT 1 cut(s) 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.