Rw5G005320

disease resistance protein At4g27220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002553
Physical Location & Seq
Forward (+)
5535781 .. 5539253
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G005320.1

Sequence Viewer

Length: 534 bp
ATGACTGATGGATTGCCCTTGGCTATTGGCTTTCTGGCACAGACCTTGAAAGAATTAAAGATTGATGGAGCTGACAGTGTCAATGCTGCTTGGGATGCAGCCCTCTTGAAGTTACAGAGTTCTCACTTGGAAAGCATGAATGAAAAGGCATTTGCTCGTCTGAAGTACAGCTATGATATGTTGAAGAAAGATGCTCAGCAATGTCTCCTGTATTGCGCCTTATACCCATGTGGTCTGGTAAAAAAAGAGCTAGTGGAGTACTGGTTTTGGGAGGGTTTATTGGATAGAAACAAGGGAGACGGACGGATAGAAGATATGGTACAAGCAAGAGAGATACTCGATGAGCTCATAGGAGCTTACCTTCTAGAAACAGTTAGTCAAGAAGGTGGCAGGGAATGCATAAAGATGCGCAGATGGAAGGAGGAGGATGGAGTTGAGGAGGATAGTAGTGTGGTGGTGACGTTGGTGCCGAAGTTTGGGAAAGGGCCTATCACCATTGGTGGGTTGAAGATCGAGCTTATTACTACCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

177

Amino Acids

19.86

Weight (kDa)

4.89

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPO1_KFDV PF12143 144 - 173 1.2e-06 Chloroplastic Polyphenol Oxidase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 466
AcuI CTGAAG 1 cut(s) 182
AfaI GTAC 3 cut(s) 167, 260, 321
AfiI CCNNNNNNNGG 2 cut(s) 476, 501
AgsI TTSAA 4 cut(s) 49, 109, 184, 508
AluBI AGCT 6 cut(s) 71, 171, 250, 346, 356, 517
AluI AGCT 6 cut(s) 71, 171, 250, 346, 356, 517
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 209, 291
AoxI GGCC 1 cut(s) 485
ApeKI GCWGC 2 cut(s) 86, 98
AspLEI GCGC 2 cut(s) 218, 411
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 2 cut(s) 469, 484
BanI GGYRCC 1 cut(s) 466
BanII GRGCYC 1 cut(s) 348
BarI GAAGNNNNNNTAC 2 cut(s) 303, 335
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 73, 110
BccI CCATC 4 cut(s) 2, 59, 408, 422
BcoDI GTCTC 2 cut(s) 209, 291
BfaI CTAG 2 cut(s) 251, 365
BisI GCNGC 2 cut(s) 87, 99
BlpI GCTNAGC 1 cut(s) 195
BlsI GCNGC 2 cut(s) 88, 100
BmcAI AGTACT 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 468
BmsI GCATC 3 cut(s) 85, 181, 396
BplI GAGNNNNNCTC 2 cut(s) 321, 353
Bpu1102I GCTNAGC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 18
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 501
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 2 cut(s) 100, 433
BseLI CCNNNNNNNGG 2 cut(s) 476, 501
BseMI GCAATG 1 cut(s) 206
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 2 cut(s) 437, 452
BseXI GCAGC 2 cut(s) 73, 110
BshFI GGCC 1 cut(s) 487
BshNI GGYRCC 1 cut(s) 466
BsiHKAI GWGCWC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 476, 501
BsmAI GTCTC 2 cut(s) 209, 291
BsmBI CGTCTC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 1 cut(s) 510
Bsp1720I GCTNAGC 1 cut(s) 195
BspANI GGCC 1 cut(s) 487
BspCNI CTCAG 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 466
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 1 cut(s) 510
BssT1I CCWWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 77, 373
BstAPI GCANNNNNTGC 1 cut(s) 396
BstDEI CTNAG 1 cut(s) 195
BstF5I GGATG 2 cut(s) 100, 433
BstHHI GCGC 2 cut(s) 218, 411
BstKTI GATC 1 cut(s) 513
BstMAI GTCTC 2 cut(s) 209, 291
BstMBI GATC 1 cut(s) 510
BstMWI GCNNNNNNNGC 2 cut(s) 95, 396
BstV1I GCAGC 2 cut(s) 73, 110
BsuRI GGCC 1 cut(s) 487
BtsCI GGATG 2 cut(s) 100, 433
BtsIMutI CAGTG 1 cut(s) 82
CfoI GCGC 2 cut(s) 218, 411
Cfr13I GGNCC 1 cut(s) 485
Csp6I GTAC 3 cut(s) 166, 259, 320
CviAII CATG 2 cut(s) 136, 228
CviQI GTAC 3 cut(s) 166, 259, 320
DdeI CTNAG 1 cut(s) 195
DpnI GATC 1 cut(s) 512
DpnII GATC 1 cut(s) 510
Ecl136II GAGCTC 1 cut(s) 346
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 182
EcoICRI GAGCTC 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 485
EcoT14I CCWWGG 1 cut(s) 18
EcoT22I ATGCAT 1 cut(s) 401
EcoT38I GRGCYC 1 cut(s) 348
ErhI CCWWGG 1 cut(s) 18
Esp3I CGTCTC 1 cut(s) 291
FaeI CATG 2 cut(s) 139, 231
FaiI YATR 8 cut(s) 137, 174, 179, 223, 229, 317, 350, 401
FatI CATG 2 cut(s) 135, 227
Fnu4HI GCNGC 2 cut(s) 87, 99
FokI GGATG 2 cut(s) 107, 440
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 2 cut(s) 87, 99
FspBI CTAG 2 cut(s) 251, 365
FspI TGCGCA 1 cut(s) 410
GlaI GCGC 2 cut(s) 217, 410
GluI GCNGC 2 cut(s) 87, 99
HaeIII GGCC 1 cut(s) 487
HhaI GCGC 2 cut(s) 218, 411
Hin1II CATG 2 cut(s) 139, 231
Hin6I GCGC 2 cut(s) 216, 409
HinP1I GCGC 2 cut(s) 216, 409
HphI GGTGA 2 cut(s) 469, 484
Hpy188I TCNGA 1 cut(s) 162
Hpy188III TCNNGA 3 cut(s) 106, 365, 380
HpyAV CCTTC 3 cut(s) 371, 377, 412
HpyCH4III ACNGT 2 cut(s) 77, 373
HpyCH4IV ACGT 1 cut(s) 461
HpyCH4V TGCA 2 cut(s) 98, 399
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 396
HpyF3I CTNAG 1 cut(s) 195
HpySE526I ACGT 1 cut(s) 461
Hsp92II CATG 2 cut(s) 139, 231
HspAI GCGC 2 cut(s) 216, 409
Kzo9I GATC 1 cut(s) 510
LmnI GCTCC 2 cut(s) 68, 353
LpnPI CCDG 5 cut(s) 20, 221, 221, 247, 376
Lsp1109I GCAGC 2 cut(s) 73, 110
LweI GCATC 3 cut(s) 85, 181, 396
MaeI CTAG 2 cut(s) 251, 365
MaeII ACGT 1 cut(s) 461
MaeIII GTNAC 2 cut(s) 111, 457
MalI GATC 1 cut(s) 512
MboI GATC 1 cut(s) 510
MboII GAAGA 3 cut(s) 196, 323, 520
MhlI GDGCHC 1 cut(s) 348
MluCI AATT 1 cut(s) 53
MnlI CCTC 6 cut(s) 113, 265, 415, 418, 430, 433
Mph1103I ATGCAT 1 cut(s) 401
MseI TTAA 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 404
Mva1269I GAATGC 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 95, 396
NdeII GATC 1 cut(s) 510
NlaIII CATG 2 cut(s) 139, 231
NlaIV GGNNCC 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 457
NsbI TGCGCA 1 cut(s) 410
NsiI ATGCAT 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 467
PctI GAATGC 1 cut(s) 401
PflFI GACNNNGTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 88, 100
Psp124BI GAGCTC 1 cut(s) 348
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 485
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 3 cut(s) 167, 260, 321
RsaNI GTAC 3 cut(s) 166, 259, 320
RseI CAYNNNNRTG 1 cut(s) 404
SacI GAGCTC 1 cut(s) 348
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 2 cut(s) 87, 99
Sau3AI GATC 1 cut(s) 510
Sau96I GGNCC 1 cut(s) 485
ScaI AGTACT 1 cut(s) 260
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 3 cut(s) 85, 181, 396
SmiMI CAYNNNNRTG 1 cut(s) 404
Sse9I AATT 1 cut(s) 53
SspMI CTAG 2 cut(s) 251, 365
SstI GAGCTC 1 cut(s) 348
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 77, 373
TaiI ACGT 1 cut(s) 464
TaqI TCGA 2 cut(s) 339, 513
TasI AATT 1 cut(s) 53
TatI WGTACW 2 cut(s) 165, 258
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TscAI CASTG 1 cut(s) 82
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 2 cut(s) 86, 98
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 152, 156
TspGWI ACGGA 2 cut(s) 315, 319
TspRI CASTG 1 cut(s) 82
Tth111I GACNNNGTC 1 cut(s) 77
XbaI TCTAGA 1 cut(s) 364
XspI CTAG 2 cut(s) 251, 365
ZrmI AGTACT 1 cut(s) 260
Zsp2I ATGCAT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.