Rorug04G0423500

disease resistance protein At4g27220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002553
Physical Location & Seq
Forward (+)
58267556 .. 58268101
546 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0423500.1

Sequence Viewer

Length: 417 bp
ATGCCTTTTTCTCAAACCCCATCCCGCATGATGTACGATGCGATATCATCGAGCCTCACGGATATGGAAAAAGAGGGATTGATTGATGAAGGTCAAGTGGATGCTTTCAACTTGCCTTTCTACGCTGCCTCACTGGAGGAGTTGGAGGGGCTTGTGGTTAAAAATGGGTGCTTTAGCATCGAGAGGATGGAGTCTTCAAACCCAGCAGCATGGTTGAAAGGTCCAGTTGACATACCACAATGGGTGATGCATATACGGGCTGCAATGGAAGGAATGTTCGCCGGACATTTTGGAAGTCATGAGGTTATGGATCAAATGTTCCAACGTTCGGCTCAAAAGCTTTCAGACAAGTCTGACCTTCTAAACTCAAGGTGCCACGAGCAAAGTCAGCTGCTTGCTGTTCTCAAGCGTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

138

Amino Acids

15.53

Weight (kDa)

5.14

Isoelectric Point (pI)

58.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 2 - 137 5.9e-27 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 372
AciI CCGC 1 cut(s) 25
AclI AACGTT 1 cut(s) 325
AclWI GGATC 1 cut(s) 318
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 3 cut(s) 109, 198, 217
AloI GAACNNNNNNTCC 4 cut(s) 260, 292, 302, 334
AluBI AGCT 2 cut(s) 340, 391
AluI AGCT 2 cut(s) 340, 391
AlwI GGATC 1 cut(s) 318
ApeKI GCWGC 4 cut(s) 125, 206, 260, 391
AspS9I GGNCC 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 256
AvaII GGWCC 1 cut(s) 221
BanI GGYRCC 1 cut(s) 372
BauI CACGAG 1 cut(s) 377
BbsI GAAGAC 1 cut(s) 186
BbvI GCAGC 4 cut(s) 112, 218, 247, 378
BccI CCATC 2 cut(s) 28, 181
BisI GCNGC 4 cut(s) 126, 207, 261, 392
BlsI GCNGC 4 cut(s) 127, 208, 262, 393
Bme18I GGWCC 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 221
BmiI GGNNCC 1 cut(s) 374
BmsI GCATC 4 cut(s) 28, 91, 186, 237
BpiI GAAGAC 1 cut(s) 186
BpmI CTGGAG 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 352, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 138, 224
Bse3DI GCAATG 1 cut(s) 270
BseGI GGATG 3 cut(s) 20, 106, 192
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 2 cut(s) 138, 224
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 4 cut(s) 112, 218, 247, 378
BseYI CCCAGC 1 cut(s) 202
BshNI GGYRCC 1 cut(s) 372
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 328
Bsp143I GATC 1 cut(s) 310
BspACI CCGC 1 cut(s) 25
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 1 cut(s) 374
BspPI GGATC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 270
BsrI ACTGG 2 cut(s) 138, 224
BssMI GATC 1 cut(s) 310
BssSI CACGAG 1 cut(s) 377
Bst2BI CACGAG 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 396
BstF5I GGATG 3 cut(s) 20, 106, 192
BstKTI GATC 1 cut(s) 313
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstV1I GCAGC 4 cut(s) 112, 218, 247, 378
BstV2I GAAGAC 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 210
BtsCI GGATG 3 cut(s) 20, 106, 192
BtsIMutI CAGTG 1 cut(s) 131
Cac8I GCNNGC 1 cut(s) 396
CciI TCATGA 1 cut(s) 298
Cfr13I GGNCC 1 cut(s) 221
Csp6I GTAC 1 cut(s) 34
CviAII CATG 3 cut(s) 28, 210, 299
CviJI RGCY 6 cut(s) 54, 151, 260, 332, 340, 391
CviKI_1 RGCY 6 cut(s) 54, 151, 260, 332, 340, 391
CviQI GTAC 1 cut(s) 34
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eco32I GATATC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 221
EcoRV GATATC 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 252
FaeI CATG 3 cut(s) 31, 213, 302
FaiI YATR 8 cut(s) 29, 65, 211, 233, 252, 254, 300, 308
FatI CATG 3 cut(s) 27, 209, 298
FauI CCCGC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 126, 207, 261, 392
FokI GGATG 3 cut(s) 7, 113, 199
Fsp4HI GCNGC 4 cut(s) 126, 207, 261, 392
GluI GCNGC 4 cut(s) 126, 207, 261, 392
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 155
HapII CCGG 1 cut(s) 282
Hin1II CATG 3 cut(s) 31, 213, 302
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 338
HinfI GANTC 1 cut(s) 191
HpaII CCGG 1 cut(s) 282
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 346, 355
Hpy188III TCNNGA 2 cut(s) 181, 299
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 3 cut(s) 83, 263, 368
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 2 cut(s) 250, 263
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 3 cut(s) 31, 213, 302
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 4 cut(s) 119, 216, 237, 295
Lsp1109I GCAGC 4 cut(s) 112, 218, 247, 378
LweI GCATC 4 cut(s) 28, 91, 186, 237
MaeII ACGT 1 cut(s) 325
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 1 cut(s) 186
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 123, 346
MnlI CCTC 7 cut(s) 65, 67, 130, 139, 139, 177, 295
Mph1103I ATGCAT 1 cut(s) 252
MseI TTAA 1 cut(s) 159
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 1 cut(s) 282
MwoI GCNNNNNNNGC 1 cut(s) 388
NdeII GATC 1 cut(s) 310
NlaIII CATG 3 cut(s) 31, 213, 302
NlaIV GGNNCC 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 252
PagI TCATGA 1 cut(s) 298
PkrI GCNGC 4 cut(s) 127, 208, 262, 393
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Psp1406I AACGTT 1 cut(s) 325
PspFI CCCAGC 1 cut(s) 202
PspN4I GGNNCC 1 cut(s) 374
PspPI GGNCC 1 cut(s) 221
PvuII CAGCTG 1 cut(s) 391
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 62
SaqAI TTAA 1 cut(s) 159
SatI GCNGC 4 cut(s) 126, 207, 261, 392
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 1 cut(s) 221
SchI GAGTC 1 cut(s) 200
SetI ASST 8 cut(s) 94, 223, 306, 328, 342, 360, 374, 393
SfaNI GCATC 4 cut(s) 28, 91, 186, 237
SinI GGWCC 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 2 cut(s) 367, 404
SmoI CTYRAG 2 cut(s) 367, 404
SsiI CCGC 1 cut(s) 25
TaiI ACGT 1 cut(s) 328
TaqI TCGA 2 cut(s) 50, 180
Tru1I TTAA 1 cut(s) 159
Tru9I TTAA 1 cut(s) 159
TscAI CASTG 1 cut(s) 138
TseI GCWGC 4 cut(s) 125, 206, 260, 391
TspDTI ATGAA 1 cut(s) 102
TspGWI ACGGA 1 cut(s) 74
TspRI CASTG 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.