Rorug04G0423600

disease resistance protein At4g27220

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002553
Physical Location & Seq
Reverse (-)
58268506 .. 58273993
5488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0423600.1

Sequence Viewer

Length: 3381 bp
ATGGGGAAGACAATACAGTTATATGGATTCCCTTCAGGTGAATCTGCCGAAAAAATTAAGGTATTTTTGGAGAAACATACAGGAGAAGGAAGTGTTTTTGCTCTTAAATTACGGCCGGCCAAAAACGGGGGAAGATTCTATGCTATCATACAGTTTCAGTCCACCAGTCAGGCTGAGCTTATCACCACTTTGGCTAAAGCTAGGCTGTGGTATGGGAGATCATATTTGAACGCTCGTCCGGTGGACCTTGATATTGTACCAAAGCCAAGAACTTTTATGCATAGCTTGCAAAATTTAGTATTGCATGTTGGCTGCCAGGTTGCAAATGATAAGTTGTCTGTTTTGTGGAGAACGGCTGAAGTTTCAGTGGACTTTGGGATTGGAATGCGGAAATTGCAATTTCGGCTGTTATATCGTCAGAAGTTATATAGACTTCAGCTCTCTTATGAGAACATCTGGCAAATTGAGTTGCATCGTCCACGTGGTCGCACTGCAAAATATCTTGTCATTCAGTTATATGGAGCTCCTCGCATCTACCAACAAGAAGAAGCATGTTCTTCTGGAAATGTATATGATGACCCTCTTATGAACTACTTTAAGGATGTCCCTGATGACCAATGGGTTCGGACTACTGATTTTACTCCACGAAGCTGTATTGGGGAGTCATCTTCATTATGTTTAGAGCTTCCTTCTGGTCGCCAACTTCCTGATTTTGAAGAAAATTTTGCTTATTATAAGGAGAGTGATGGGCAATTTATCTTGGAGAATGGCTCAACTTTCTCTTGCAACCAAGATCTGGTCCCTATTGTTGGTTCTCCTGCAGGGGTTGATTTGCCATATGATATCTTGTTTAAAATAAACTTGCTGGTTCAAATGGGTTGTCTATCAGGGCCGACACTGGATAATAGTTTTTATAGGCTGGTGGATCCACGAAGAATAACTGATTGTTCCTGTATAGAGTATGCCCTAGATAAATTATCTAATTTGAAAGAGAACTGTTATGAACCTGCAGTGTGGCTTCGTGAGGAATATCGGAAGTACCTGACATCAAAGAAACCTCCAAAGCCGGCACCTATCTCATTAGATTCTGGTTTGGTATATGTACGCAGGGTTCAAATAACACCATCTAAAGTGTATTTCTCTGGTCCTGAGGTTAATGTCTCAAATCGCATACTACGCCAGTATTCCAGTGATAACGATAACTTTCTTCGTGTTTCATTTGTTGATGAGGAGATGGAAAAACTCTATTCCACAGATTTATCCCCCCGTACATCTACTAGGAAAACTCGCATCTACAACAGAATTCTTTCTGTTCTCAGAAACGGCATAGTAATTGGAGAAAAGAAATTTGATTTTCTTGCATTCTCATCAAGTCAATTGCGAGAGAATTCGTTGTGGATGTTTGCTTCAACCTATAAATTTAGTGCTGAGAAGATAAGAGCAGGGATGGGGGATTTTCGCAAGATAAGAAATGTGGCAAAGTATGCTGCTAGATTGGGTCAATCTTTTGGCTCTTCTACTGAAACTCTAAGTGTTGGCAGGCATGAAATTGAAGTTATTCCTGATGTAACCGTTAACTGGGGTGGAGACAAATATGTGTTCTCCGATGGGATTGGGAAAATATCAGCTGATTTTGCAACGAGAGTAGCCTTCAAATGTGGCTATAAAGCGTTCACACCATCTGCATTTCAAATTCGATATGGTGGTTACAAAGGTGTTGTAGCTGTTGATCCAACCTCGTCAATGAAATTATCACTGAGAAAGAGCATGTCCAAGTATGATTCTGAAAACACCAAGCTAGATGTTCTAGCATGTAGCAAGTTTCAGCCTTGTTTTTTGAATCGTCAGTTGATAACTCTTCTGTCTACACTTGGAGTTCAAGATCATGTTTTCATGAAAAAGCAAAGAGCAGCTGTACAACAACTTAATGCCATATTGACTGACCCATTAAAAGCAGGGGAGGCTCTTGATTTGATGTCTGCGGGTGAGAGTACAAACATCCTCAAGGAACTGCTGATGTGTGGTTATAAACCTGATGTTGAACCATTCCTTTCAATGATGCTCCAAACATTTCGGGCATCAAAGTTGTTGGAATTGAGGACCAAAACAAGAATCTTTATCCCAGACGGAAGAGCAATGATGGGATGTCTAGACGAAACAAGAACCTTGGAATATGGGCAGGTGTTTGTGCAAATCTCTGGAAACAGACAAAGGCAGCTATTTGATGATTCATTCATGCATGGTGGAAGCTCATCAGGCAACTGTTTTGTTATTGAGGGAAAGGTGGTTGTTGCAAAGAACCCATGTTTACACCCTGGTGATGTGCGTGTTTTAAGGGCTGTGAATGTGCCAGAGTTACACCATATGGTGGACTGTGTGGTATTTCCACAAAAAGGACCAAGGCCTCATCCAAATGAATGTTCTGGAAGTGATCTTGATGGAGATATTTACTTTGTTTGCTGGGACCCTGAGTTGATTCCTCGCAATCAAATCCAACCTATGGATTATACCCCAGCACCACCTGCAGAGGTGGATCATGATGTCACAATTGAGGAAGTAGAAGAGTACTTTGTGAATTACATACTCAATGATAGTTTGGGGATAATTGCCAATGCCCACACTGTTTTTGCTGACAGAGAGCATGGTAAAGCAATGAGCCCTCCATGCATAAAGCTCGCGAAGCTATTTTCAATTGCTGTAGACTTTCCAAAAACTGGCGTGCCAGCTGTGATACCACCAGAACTGTATGTCAAGGAATATCCAGATTTCATGGAGAAACCCGACAAAAGCACCTATCTTTCAACCAATGTGATCGGACAGCTATTTCGTGAGGTGAAGGATATTGCACCCCATGACAGCTGTATTACATCCTTCACTCTTCAAGTGGCGACAAAATCCTATGACCCTGACATGGAGGTTGATGGCTTTGAGGATTACCTTGAAGATGCTTTCTATTACAAAGGCAATTATGATTACAAATTGGGAAACCTGTTGGATTACTATGGGATCAAGACAGAGGCTGAAATATTGAGTGGGAGTATTATGAGCATGTCAAAATCTTTCACCAAGAGGCGGGATGCAGATGCTATAAACATGGCTGTGAAGTCATTGAGGAAGGAAGCTAGGGCCTGGTTCAATGAGAAAGGAACTGGAATAGATTCTGGCGATGATGTCTATGCAAAAGCCTCAGCTTGGTATTATGTCACATATCATCCCAAGTATTTTGGTTTGTACAATGAGGGAATGAAACGTGACCATTTTATCAGCTTTCCATGGTGTGTTTATGACAAGCTGGTTCACATAAAAAAGGACAAAGTAAGGCAAGCTCGGCGGATATCATCTCTAGAAAGTCAGTTGAGGTACGGCTTGCATTTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

1126

Amino Acids

127.92

Weight (kDa)

8.21

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_RdRP1_2 PF26250 4 - 81 4.4e-17 RdRP1/2, RRM domain
RDR6_2nd PF24577 96 - 247 3.5e-06 RNA-dependent RNA polymerase 6 second domain
PH_RDR2 PF24823 101 - 252 8.1e-46 RDR2 PH-like domain
RdRP_helical PF26252 273 - 352 3.5e-11 RNA dependent RNA polymerase, helical domain
RdRP PF05183 373 - 945 6.4e-197 RNA dependent RNA polymerase
RdRP_head PF26253 966 - 1110 1.6e-48 RdRP, head domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 735, 2028
AarI CACCTGC 2 cut(s) 2170, 2530
Acc36I ACCTGC 3 cut(s) 1015, 2170, 2530
AccB1I GGYRCC 1 cut(s) 1069
AccB7I CCANNNNNTGG 4 cut(s) 796, 2368, 2500, 2714
AccI GTMKAC 2 cut(s) 1865, 2700
AccII CGCG 1 cut(s) 2678
AciI CCGC 4 cut(s) 388, 1982, 3073, 3331
AclWI GGATC 5 cut(s) 920, 933, 1724, 2541, 3014
AcoI YGGCCR 2 cut(s) 113, 117
AcsI RAATTY 7 cut(s) 292, 721, 1302, 1346, 1387, 1418, 1692
AcuI CTGAAG 3 cut(s) 18, 378, 419
AcvI CACGTG 1 cut(s) 482
AfaI GTAC 9 cut(s) 258, 1040, 1104, 1270, 1917, 1993, 2567, 3233, 3362
AjnI CCWGG 3 cut(s) 315, 2314, 3128
AjuI GAANNNNNNNTTGG 2 cut(s) 2404, 2436
AleI CACNNNNGTG 1 cut(s) 2316
Alw21I GWGCWC 1 cut(s) 526
Alw26I GTCTC 2 cut(s) 1165, 1581
AlwI GGATC 5 cut(s) 920, 933, 1724, 2541, 3014
AlwNI CAGNNNCTG 1 cut(s) 3020
AoxI GGCC 5 cut(s) 113, 117, 890, 2402, 3126
ApeKI GCWGC 4 cut(s) 312, 1487, 1910, 2215
ApoI RAATTY 7 cut(s) 292, 721, 1302, 1346, 1387, 1418, 1692
ArsI GACNNNNNNTTYG 4 cut(s) 865, 897, 2808, 2840
Asp700I GAANNNNTTC 3 cut(s) 1306, 1557, 3157
AspS9I GGNCC 8 cut(s) 244, 799, 890, 1145, 2100, 2396, 2464, 3126
AsuHPI GGTGA 6 cut(s) 50, 175, 1997, 2330, 2845, 3055
AvaII GGWCC 6 cut(s) 244, 799, 1145, 2100, 2396, 2464
AxyI CCTNAGG 1 cut(s) 1149
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 1069
BanII GRGCYC 2 cut(s) 526, 2660
BbrPI CACGTG 1 cut(s) 482
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 526
BbvCI CCTCAGC 1 cut(s) 3187
BbvI GCAGC 4 cut(s) 299, 1474, 1922, 2227
BccI CCATC 9 cut(s) 740, 1132, 1228, 1441, 1601, 1687, 2134, 2432, 2915
BceAI ACGGC 4 cut(s) 128, 369, 1339, 3379
BcgI CGANNNNNNTGC 2 cut(s) 2656, 2690
BciT130I CCWGG 3 cut(s) 317, 2316, 3130
BcoDI GTCTC 2 cut(s) 1165, 1581
BfaI CTAG 9 cut(s) 201, 968, 1278, 1491, 1799, 1808, 2150, 3123, 3344
BfmI CTRYAG 4 cut(s) 819, 1008, 2523, 2697
BfuAI ACCTGC 3 cut(s) 1015, 2170, 2530
BglII AGATCT 1 cut(s) 793
BisI GCNGC 4 cut(s) 313, 1488, 1911, 2216
BlpI GCTNAGC 1 cut(s) 174
BlsI GCNGC 4 cut(s) 314, 1489, 1912, 2217
BmcAI AGTACT 1 cut(s) 2567
Bme1390I CCNGG 3 cut(s) 317, 2316, 3130
Bme18I GGWCC 6 cut(s) 244, 799, 1145, 2100, 2396, 2464
BmgT120I GGNCC 8 cut(s) 244, 799, 890, 1145, 2100, 2396, 2464, 3126
BmiI GGNNCC 5 cut(s) 801, 927, 1071, 2465, 2466
BmrFI CCNGG 3 cut(s) 317, 2316, 3130
BmrI ACTGGG 1 cut(s) 1588
BmsI GCATC 8 cut(s) 481, 540, 1299, 2049, 2087, 2935, 3067, 3073
BmuI ACTGGG 1 cut(s) 1588
BpiI GAAGAC 1 cut(s) 14
BplI GAGNNNNNCTC 2 cut(s) 755, 787
Bpu10I CCTNAGC 1 cut(s) 3187
Bpu1102I GCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 1988
BsaAI YACGTR 1 cut(s) 482
BsaJI CCNNGG 4 cut(s) 2166, 2314, 2399, 3272
BsaWI WCCGGW 1 cut(s) 238
Bse118I RCCGGY 2 cut(s) 115, 1066
Bse1I ACTGG 7 cut(s) 165, 903, 1180, 1188, 1583, 2719, 3154
Bse21I CCTNAGG 1 cut(s) 1149
Bse3DI GCAATG 2 cut(s) 2142, 2658
BseBI CCWGG 3 cut(s) 317, 2316, 3130
BseDI CCNNGG 4 cut(s) 2166, 2314, 2399, 3272
BseGI GGATG 9 cut(s) 607, 1404, 1452, 1998, 2150, 2407, 2867, 3082, 3211
BseMI GCAATG 2 cut(s) 2142, 2658
BseMII CTCAG 7 cut(s) 165, 1140, 1330, 1419, 1748, 2460, 3201
BseNI ACTGG 7 cut(s) 165, 903, 1180, 1188, 1583, 2719, 3154
BseRI GAGGAG 2 cut(s) 516, 1244
BseX3I CGGCCG 1 cut(s) 113
BseXI GCAGC 4 cut(s) 299, 1474, 1922, 2227
BseYI CCCAGC 2 cut(s) 2460, 2512
Bsh1236I CGCG 1 cut(s) 2678
Bsh1285I CGRYCG 1 cut(s) 116
BshFI GGCC 5 cut(s) 115, 119, 892, 2404, 3128
BshNI GGYRCC 1 cut(s) 1069
BsiEI CGRYCG 1 cut(s) 116
BsiHKAI GWGCWC 1 cut(s) 526
BsiSI CCGG 3 cut(s) 116, 239, 1067
BslFI GGGAC 3 cut(s) 590, 785, 2477
BsmAI GTCTC 2 cut(s) 1165, 1581
BsmFI GGGAC 3 cut(s) 590, 785, 2477
BsmI GAATGC 2 cut(s) 390, 1361
BsnI GGCC 5 cut(s) 115, 119, 892, 2404, 3128
Bsp1286I GDGCHC 2 cut(s) 526, 2660
Bsp1407I TGTACA 2 cut(s) 1915, 3231
Bsp143I GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
Bsp1720I GCTNAGC 1 cut(s) 174
Bsp19I CCATGG 1 cut(s) 3272
Bsp68I TCGCGA 1 cut(s) 2678
BspACI CCGC 4 cut(s) 388, 1982, 3073, 3331
BspANI GGCC 5 cut(s) 115, 119, 892, 2404, 3128
BspCNI CTCAG 7 cut(s) 166, 1141, 1329, 1420, 1749, 2461, 3200
BspFNI CGCG 1 cut(s) 2678
BspHI TCATGA 2 cut(s) 1893, 2536
BspLI GGNNCC 5 cut(s) 801, 927, 1071, 2465, 2466
BspMAI CTGCAG 3 cut(s) 823, 1012, 2527
BspMI ACCTGC 3 cut(s) 1015, 2170, 2530
BspPI GGATC 5 cut(s) 920, 933, 1724, 2541, 3014
BspQI GCTCTTC 2 cut(s) 1519, 2125
BspT107I GGYRCC 1 cut(s) 1069
BsrDI GCAATG 2 cut(s) 2142, 2658
BsrFI RCCGGY 2 cut(s) 115, 1066
BsrGI TGTACA 2 cut(s) 1915, 3231
BsrI ACTGG 7 cut(s) 165, 903, 1180, 1188, 1583, 2719, 3154
BssAI RCCGGY 2 cut(s) 115, 1066
BssECI CCNNGG 4 cut(s) 2166, 2314, 2399, 3272
BssMI GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
BssT1I CCWWGG 3 cut(s) 2166, 2399, 3272
Bst2UI CCWGG 3 cut(s) 317, 2316, 3130
Bst4CI ACNGT 8 cut(s) 18, 153, 998, 1573, 2264, 2375, 2623, 2745
Bst6I CTCTTC 5 cut(s) 1519, 1863, 2125, 2556, 2883
BstAPI GCANNNNNTGC 3 cut(s) 286, 1484, 2522
BstAUI TGTACA 2 cut(s) 1915, 3231
BstBAI YACGTR 1 cut(s) 482
BstC8I GCNNGC 9 cut(s) 117, 287, 1068, 1541, 2676, 2720, 2724, 3324, 3368
BstDEI CTNAG 8 cut(s) 174, 1149, 1316, 1428, 1529, 1757, 2469, 3187
BstDSI CCRYGG 1 cut(s) 3272
BstF5I GGATG 9 cut(s) 607, 1404, 1452, 1998, 2150, 2407, 2867, 3082, 3211
BstFNI CGCG 1 cut(s) 2678
BstKTI GATC 9 cut(s) 221, 796, 928, 1732, 1885, 2434, 2536, 2814, 3009
BstMAI GTCTC 2 cut(s) 1165, 1581
BstMBI GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
BstMCI CGRYCG 1 cut(s) 116
BstNI CCWGG 3 cut(s) 317, 2316, 3130
BstNSI RCATGY 5 cut(s) 308, 555, 1771, 1815, 3052
BstSCI CCNGG 3 cut(s) 315, 2314, 3128
BstSFI CTRYAG 4 cut(s) 819, 1008, 2523, 2697
BstUI CGCG 1 cut(s) 2678
BstV1I GCAGC 4 cut(s) 299, 1474, 1922, 2227
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 2 cut(s) 793, 925
BstYI RGATCY 2 cut(s) 793, 925
BstZI CGGCCG 1 cut(s) 113
Bsu36I CCTNAGG 1 cut(s) 1149
BsuRI GGCC 5 cut(s) 115, 119, 892, 2404, 3128
BtgI CCRYGG 1 cut(s) 3272
BtgZI GCGATG 1 cut(s) 3180
BtsCI GGATG 9 cut(s) 607, 1404, 1452, 1998, 2150, 2407, 2867, 3082, 3211
BtsI GCAGTG 2 cut(s) 489, 1017
BtsIMutI CAGTG 7 cut(s) 372, 489, 896, 1017, 1195, 1754, 2619
BtuMI TCGCGA 1 cut(s) 2678
BveI ACCTGC 3 cut(s) 1015, 2170, 2530
Cac8I GCNNGC 9 cut(s) 117, 287, 1068, 1541, 2676, 2720, 2724, 3324, 3368
CaiI CAGNNNCTG 1 cut(s) 3020
CciI TCATGA 2 cut(s) 1893, 2536
Cfr10I RCCGGY 2 cut(s) 115, 1066
Cfr13I GGNCC 8 cut(s) 244, 799, 890, 1145, 2100, 2396, 2464, 3126
Csp6I GTAC 9 cut(s) 257, 1039, 1103, 1269, 1916, 1992, 2566, 3232, 3361
CviQI GTAC 9 cut(s) 257, 1039, 1103, 1269, 1916, 1992, 2566, 3232, 3361
DdeI CTNAG 8 cut(s) 174, 1149, 1316, 1428, 1529, 1757, 2469, 3187
DpnI GATC 9 cut(s) 220, 795, 927, 1731, 1884, 2433, 2535, 2813, 3008
DpnII GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
DraI TTTAAA 1 cut(s) 853
EaeI YGGCCR 2 cut(s) 113, 117
EagI CGGCCG 1 cut(s) 113
Eam1104I CTCTTC 5 cut(s) 1519, 1863, 2125, 2556, 2883
EarI CTCTTC 5 cut(s) 1519, 1863, 2125, 2556, 2883
EciI GGCGGA 1 cut(s) 3346
Ecl136II GAGCTC 1 cut(s) 524
EclXI CGGCCG 1 cut(s) 113
Eco130I CCWWGG 3 cut(s) 2166, 2399, 3272
Eco147I AGGCCT 1 cut(s) 2404
Eco24I GRGCYC 2 cut(s) 526, 2660
Eco32I GATATC 2 cut(s) 844, 3336
Eco47I GGWCC 6 cut(s) 244, 799, 1145, 2100, 2396, 2464
Eco52I CGGCCG 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 524
Eco57I CTGAAG 3 cut(s) 18, 378, 419
Eco72I CACGTG 1 cut(s) 482
Eco81I CCTNAGG 1 cut(s) 1149
EcoICRI GAGCTC 1 cut(s) 524
EcoO109I RGGNCCY 2 cut(s) 2464, 3126
EcoRI GAATTC 2 cut(s) 1302, 1387
EcoRII CCWGG 3 cut(s) 315, 2314, 3128
EcoRV GATATC 2 cut(s) 844, 3336
EcoT14I CCWWGG 3 cut(s) 2166, 2399, 3272
EcoT22I ATGCAT 3 cut(s) 282, 2241, 2669
EcoT38I GRGCYC 2 cut(s) 526, 2660
ErhI CCWWGG 3 cut(s) 2166, 2399, 3272
FaqI GGGAC 3 cut(s) 590, 785, 2477
FauI CCCGC 2 cut(s) 1975, 3066
FauNDI CATATG 2 cut(s) 838, 2364
FblI GTMKAC 2 cut(s) 1865, 2700
Fnu4HI GCNGC 4 cut(s) 313, 1488, 1911, 2216
FokI GGATG 9 cut(s) 614, 1411, 1459, 1985, 2157, 2394, 2854, 3089, 3198
FriOI GRGCYC 2 cut(s) 526, 2660
FseI GGCCGGCC 1 cut(s) 119
Fsp4HI GCNGC 4 cut(s) 313, 1488, 1911, 2216
FspBI CTAG 9 cut(s) 201, 968, 1278, 1491, 1799, 1808, 2150, 3123, 3344
GluI GCNGC 4 cut(s) 313, 1488, 1911, 2216
GsaI CCCAGC 2 cut(s) 2464, 2516
HaeIII GGCC 5 cut(s) 115, 119, 892, 2404, 3128
HapII CCGG 3 cut(s) 116, 239, 1067
HincII GTYRAC 1 cut(s) 1576
HindII GTYRAC 1 cut(s) 1576
HpaI GTTAAC 1 cut(s) 1576
HpaII CCGG 3 cut(s) 116, 239, 1067
HphI GGTGA 6 cut(s) 50, 175, 1997, 2330, 2845, 3055
Hpy188I TCNGA 8 cut(s) 420, 627, 1035, 1319, 1606, 1786, 2816, 3380
HpyAV CCTTC 7 cut(s) 42, 80, 699, 1660, 2830, 2881, 3109
HpyCH4III ACNGT 8 cut(s) 18, 153, 998, 1573, 2264, 2375, 2623, 2745
HpyCH4IV ACGT 2 cut(s) 481, 3250
HpyF3I CTNAG 8 cut(s) 174, 1149, 1316, 1428, 1529, 1757, 2469, 3187
HpySE526I ACGT 2 cut(s) 481, 3250
KflI GGGWCCC 1 cut(s) 2464
KroI GCCGGC 2 cut(s) 115, 1066
KroNI GCCGGC 2 cut(s) 117, 1068
KspAI GTTAAC 1 cut(s) 1576
Kzo9I GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
LguI GCTCTTC 2 cut(s) 1519, 2125
LmnI GCTCC 3 cut(s) 521, 529, 2067
Lsp1109I GCAGC 4 cut(s) 299, 1474, 1922, 2227
LweI GCATC 8 cut(s) 481, 540, 1299, 2049, 2087, 2935, 3067, 3073
MaeI CTAG 9 cut(s) 201, 968, 1278, 1491, 1799, 1808, 2150, 3123, 3344
MaeII ACGT 2 cut(s) 481, 3250
MaeIII GTNAC 6 cut(s) 1567, 1706, 2355, 2542, 3202, 3251
MalI GATC 9 cut(s) 220, 795, 927, 1731, 1884, 2433, 2535, 2813, 3008
MboI GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
MfeI CAATTG 3 cut(s) 1376, 2547, 2691
MflI RGATCY 2 cut(s) 793, 925
MhlI GDGCHC 2 cut(s) 526, 2660
MlyI GAGTC 1 cut(s) 671
MmeI TCCRAC 4 cut(s) 1757, 2070, 2518, 2973
Mph1103I ATGCAT 3 cut(s) 282, 2241, 2669
MroNI GCCGGC 2 cut(s) 115, 1066
MroXI GAANNNNTTC 3 cut(s) 1306, 1557, 3157
MseI TTAA 9 cut(s) 57, 105, 597, 852, 1155, 1575, 1926, 1949, 2333
MslI CAYNNNNRTG 3 cut(s) 2316, 2412, 3098
MspA1I CMGCKG 4 cut(s) 1628, 1913, 2726, 2859
MspI CCGG 3 cut(s) 116, 239, 1067
MspR9I CCNGG 3 cut(s) 317, 2316, 3130
MunI CAATTG 3 cut(s) 1376, 2547, 2691
Mva1269I GAATGC 2 cut(s) 390, 1361
MvaI CCWGG 3 cut(s) 317, 2316, 3130
MvnI CGCG 1 cut(s) 2678
NaeI GCCGGC 2 cut(s) 117, 1068
NcoI CCATGG 1 cut(s) 3272
NdeI CATATG 2 cut(s) 838, 2364
NdeII GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
NgoMIV GCCGGC 2 cut(s) 115, 1066
NlaIV GGNNCC 5 cut(s) 801, 927, 1071, 2465, 2466
NmeAIII GCCGAG 1 cut(s) 3307
NmuCI GTSAC 3 cut(s) 2542, 3202, 3251
NruI TCGCGA 1 cut(s) 2678
NsiI ATGCAT 3 cut(s) 282, 2241, 2669
NspI RCATGY 5 cut(s) 308, 555, 1771, 1815, 3052
OliI CACNNNNGTG 1 cut(s) 2316
PagI TCATGA 2 cut(s) 1893, 2536
PaqCI CACCTGC 2 cut(s) 2170, 2530
PceI AGGCCT 1 cut(s) 2404
PciSI GCTCTTC 2 cut(s) 1519, 2125
PctI GAATGC 2 cut(s) 390, 1361
PdiI GCCGGC 2 cut(s) 117, 1068
PdmI GAANNNNTTC 3 cut(s) 1306, 1557, 3157
PflMI CCANNNNNTGG 4 cut(s) 796, 2368, 2500, 2714
PkrI GCNGC 4 cut(s) 314, 1489, 1912, 2217
PleI GAGTC 1 cut(s) 670
PmaCI CACGTG 1 cut(s) 482
PmlI CACGTG 1 cut(s) 482
PpsI GAGTC 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 482
PpuMI RGGWCCY 1 cut(s) 2464
PsiI TTATAA 2 cut(s) 735, 2028
Psp124BI GAGCTC 1 cut(s) 526
Psp5II RGGWCCY 1 cut(s) 2464
Psp6I CCWGG 3 cut(s) 315, 2314, 3128
PspCI CACGTG 1 cut(s) 482
PspFI CCCAGC 2 cut(s) 2460, 2512
PspGI CCWGG 3 cut(s) 315, 2314, 3128
PspN4I GGNNCC 5 cut(s) 801, 927, 1071, 2465, 2466
PspPI GGNCC 8 cut(s) 244, 799, 890, 1145, 2100, 2396, 2464, 3126
PspPPI RGGWCCY 1 cut(s) 2464
PsrI GAACNNNNNNTAC 2 cut(s) 1095, 1127
PstI CTGCAG 3 cut(s) 823, 1012, 2527
PstNI CAGNNNCTG 1 cut(s) 3020
PsuI RGATCY 2 cut(s) 793, 925
PvuII CAGCTG 4 cut(s) 1628, 1913, 2726, 2859
RigI GGCCGGCC 1 cut(s) 119
RruI TCGCGA 1 cut(s) 2678
RsaI GTAC 9 cut(s) 258, 1040, 1104, 1270, 1917, 1993, 2567, 3233, 3362
RsaNI GTAC 9 cut(s) 257, 1039, 1103, 1269, 1916, 1992, 2566, 3232, 3361
RseI CAYNNNNRTG 3 cut(s) 2316, 2412, 3098
SacI GAGCTC 1 cut(s) 526
SapI GCTCTTC 2 cut(s) 1519, 2125
SaqAI TTAA 9 cut(s) 57, 105, 597, 852, 1155, 1575, 1926, 1949, 2333
SatI GCNGC 4 cut(s) 313, 1488, 1911, 2216
Sau3AI GATC 9 cut(s) 218, 793, 925, 1729, 1882, 2431, 2533, 2811, 3006
Sau96I GGNCC 8 cut(s) 244, 799, 890, 1145, 2100, 2396, 2464, 3126
SbfI CCTGCAGG 1 cut(s) 823
ScaI AGTACT 1 cut(s) 2567
SchI GAGTC 1 cut(s) 671
ScrFI CCNGG 3 cut(s) 317, 2316, 3130
SdaI CCTGCAGG 1 cut(s) 823
SduI GDGCHC 2 cut(s) 526, 2660
SfaNI GCATC 8 cut(s) 481, 540, 1299, 2049, 2087, 2935, 3067, 3073
SfcI CTRYAG 4 cut(s) 819, 1008, 2523, 2697
SinI GGWCC 6 cut(s) 244, 799, 1145, 2100, 2396, 2464
SmiMI CAYNNNNRTG 3 cut(s) 2316, 2412, 3098
SmlI CTYRAG 1 cut(s) 2003
SmoI CTYRAG 1 cut(s) 2003
Sse8387I CCTGCAGG 1 cut(s) 823
SseBI AGGCCT 1 cut(s) 2404
SsiI CCGC 4 cut(s) 388, 1982, 3073, 3331
SspI AATATT 1 cut(s) 3027
SspMI CTAG 9 cut(s) 201, 968, 1278, 1491, 1799, 1808, 2150, 3123, 3344
SstI GAGCTC 1 cut(s) 526
StuI AGGCCT 1 cut(s) 2404
StyD4I CCNGG 3 cut(s) 315, 2314, 3128
StyI CCWWGG 3 cut(s) 2166, 2399, 3272
TaaI ACNGT 8 cut(s) 18, 153, 998, 1573, 2264, 2375, 2623, 2745
TaiI ACGT 2 cut(s) 484, 3253
TaqI TCGA 1 cut(s) 1696
TatI WGTACW 4 cut(s) 1915, 1991, 2565, 3231
Tru1I TTAA 9 cut(s) 57, 105, 597, 852, 1155, 1575, 1926, 1949, 2333
Tru9I TTAA 9 cut(s) 57, 105, 597, 852, 1155, 1575, 1926, 1949, 2333
TscAI CASTG 7 cut(s) 372, 496, 903, 1017, 1195, 1761, 2626
TseFI GTSAC 3 cut(s) 2542, 3202, 3251
TseI GCWGC 4 cut(s) 312, 1487, 1910, 2215
Tsp45I GTSAC 3 cut(s) 2542, 3202, 3251
TspGWI ACGGA 1 cut(s) 2142
TspRI CASTG 7 cut(s) 372, 496, 903, 1017, 1195, 1761, 2626
Van91I CCANNNNNTGG 4 cut(s) 796, 2368, 2500, 2714
VpaK11BI GGWCC 6 cut(s) 244, 799, 1145, 2100, 2396, 2464
XapI RAATTY 7 cut(s) 292, 721, 1302, 1346, 1387, 1418, 1692
XbaI TCTAGA 2 cut(s) 2149, 3343
XceI RCATGY 5 cut(s) 308, 555, 1771, 1815, 3052
XmiI GTMKAC 2 cut(s) 1865, 2700
XmnI GAANNNNTTC 3 cut(s) 1306, 1557, 3157
XspI CTAG 9 cut(s) 201, 968, 1278, 1491, 1799, 1808, 2150, 3123, 3344
ZrmI AGTACT 1 cut(s) 2567
Zsp2I ATGCAT 3 cut(s) 282, 2241, 2669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.