Rroxscaffold_1G00069360

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002553
Physical Location & Seq
Forward (+)
90326556 .. 90332824
6269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00069360.1

Sequence Viewer

Length: 3114 bp
ATGGGCCTCTCAACCTCTATGGGCACTCAAAGCCCAAAGACTACATCCCTCTTCGTTGGCCCGCCGTGCCACCATCGTGTACCAAAACCGGAGCCAAGTCGTTGCGCCGCCTCTCCACCTCACCGCCATTCTTTGCAGCCAAGTCCGGCAGCGACCGCACCACCGCGCAGTATCGCAGCCAACATCACGCCGCCAACCTCCAAGGTCAAGGAAAACCAAAAAACCGTTGCAGTTGTTACCAGCATCTTTGGTTTTCTAACCGTAGTGGTAACTCTGGTATCACCATGTATAAGTGAACCCCTCTCCAGGCAATGTAAAATTTACAGGAAACGCGAAGAACATCTTGAGACGTTGAAAACTGAAGTGGACACTCTGAAAGAACTAAGGAGTGAAGTAGAACGACAGTTAAAGGAGAAGGTGGTGAGAAAGTCTTTAAACAAATGTGGCTGGTTGCAAGATGCAGATAAATTTATCAGTGGCTTTAATCTGGACAATGAAAAGGTCAGAATAAGAAAGCATTGTTGTGGCCTACCAAACTGTTGGTCACAGTATAAAGCAGGAAAAGATTTTGCAGCAAGAATTGAACAGGTGAATAGACTGAAAAGTGAAGGCTCAAAGTTCATCGATGTTGAAGAATCTCAAGTCGTGGGACATATGAAAGGCAGCGGCACGAAACTACTCGGCGAGGCAGCAATAAGTATGGTTGAAGAGATTAACAAGTATGTAACAGGAAAGGAGGTCGGCACTATACGTGTTTATGGAATGGCTGGCTCTGGCAAGACAGCAGTTGTGTCGGAAGTTAATGACCTCATACTGAATGACTACAGCAGTTGTGGCCCAGAAGGTACCAATAGCGGCAGTAGATATGCTGAAGACCATTTTGACAAAGTTATTTGGGTTACTGTAGAGAATCAAGGTTCAATTCAATCATCTATTGACAACTTGCAGAATAAAATTGCAGAGAAATTACAAATTGATCTGAATAAAGCCACTGGGAGTAGAGCAGATACATTGGAAAGTGCATTGAAGGAGTTAAAATTTTTGATCATTCTGGATGATATGTGGAAGAAGCTGTCTCTTGAGCTGATTGGGATTCCAGTGCCAACGAGGGAAAATGGCTGCAAGGTTATAATAATATCCAGATCACTTCCAGTGTCCAGTGCCATTAAAATTGATAAAAGGGTCGAGAGCAAACCTCTTTCAGATACAGTGGCACGAGAAATGTTTGAGAGGGAAGCTGGCATTAGATTCTCAGACTTGGGGGATGATACTCAAACTATGGCAGAGAAGATGTTAGATGACTGTGATGATTTGCCCAGGGCTATTGGCTTTCTGGCAGAGACCTTGAAAGAATTAAAGATTGACGGAGCTGACAGTATTGATGCTGCATGGAATGCAGCCCTTCTGAAGTTAAAGAGTTCTACTGCTCACTTGGAAAGCATGAATGAAAAGGCATTTGCTCGTCTGAAGTACAGCTATGATATGTTGAAGAAAGATGCTCAGCAATGTCTCCTGTATTGTGCCTTATACCCATGTGGTCATTACATTGGTAAAAAAGAGCTAGTCGAGTATTGGTTTTGGGAGGGTTTATTGGATAGCAACAAGAGAGATGGACGGATAGAAGATATGGTACAAGCAAGAGAGATACTCGATGAGCTTGTAGGAGCTTACCTTCTGGAAACAGTTAATCAAGAAGGGGGCAAGGAATGCATAAAGATGCGCAGATTGGTTAAGCATATGGCAGTCCAGATTACCAAAGCAAGTCCTCGGTTTCTCACCAAAGCTGGGCATCAGAAAGAAAAATTCTTAGGGGATTGGCTACAGAATGTGGAGAGAGCCTCACTGATGATGAATCAGTTTAAATTCCTCGGTGGAGAACCAAAGTGTCCCAAGCTAACCACATTATTACTGCAGCATAATCCCATAGGTTTGAACTTGCATGAGAATTTCTTTCGTAGTATGCCTAACCTTCAAGTTCTTGACCTTTCTCATACTGATATCACTTCCCTTCCACAGTCATCTTTTTCTAAATTGACAAAACTCAGTGCACTTCTCTTGCATCATTGTCGCAAGTTAAAATTCCTGCCTTCTCTAGAAAAGTGTAAGAAACTACTTGTTCTCGATCTTTCTGATAGTCCCATAGCAGAATTACCAAAGGGGATGAAGAAACTAACCAAACTGCTTCGTCTAAACTTGTCTCGCACCAATGTGAAACTATTTGAAGCTAATTTAGTGCCTGCATTAGTCCATCTGGAAGAACTGTTCATGATAACTAATGAGTCTGCATCCTTGGACATCATAGTTTTCAAGGGTAACTTTCTTGTGAATGGAGGAGACTCGGTCTCACTCCCAGGACACATTGCTGAGCTGCATGTAATGAGCAATGATGATCTACAATGCTTGCAAGTTAACTACCATCTAAAGATCCTGAAGGTCATTAACATTTGCGACTGCAGATGTTTGGTCTATTCATTCACCATTGACATGTTGGGGAATCTTCCAAACCTTGAAACCATTTTAGTCGAGAAATGTCCAAAGATTGTGAGCTTGATAAAGCCAGAAAAATCTGATGACATGATTAAACCTCGTCTGAATCACCTAAAGGTCATCCGTATCTCTGACTGCGAAAGTTTGGACTACTTATTCAACAATGAGATGCTACACTACTTCCCAGAGCTCATAGAGATCTCCATCAAACGATGCCAGATGATAGAAGCTATAATAAAGTCACAAGAGCCAAATGTCTACCCTGCAATTCTTCCAAAGTTACAGGAGTTGGTATTGGTTGATCTGCAAGAATTAAAATGCATCTATAATGGAGAAATGAAGTGGAACTCTCGCCAAACAATTTGGAACTGTCCTGCTCTAAAGATGCCAATGATAACTTCTGTGCCTGCAGAACTGCTTCACAGGCCAAGTGGGAGCAATAAACTACAAAGGAGTTGTAGCTTAGATAGCAAGAAGACTATACTGCTGGACTCGATTGATATCAATGCAGCAGAAGCTCCTGAATCCAAACAGTTTGCTCTTTCTACTATATGTTCTAATAAGCAGTTCGATGTCATATGCAGGATGGAGGAGGAACTAACTATTGAAATCAGAGCACTGCAGTAG

Protein Analysis

1037

Amino Acids

116.53

Weight (kDa)

8.15

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 234 - 413 1.6e-23 NB-ARC domain
WHD_DRP PF23559 509 - 581 6.6e-07 Disease resistance protein Winged helix domain
LRR_8 PF13855 630 - 690 3.4e-10 Leucine rich repeat
LRR_14 PF23598 656 - 849 2.6e-06 Leucine-rich repeat region
LRR_RPS2 PF23247 864 - 943 7.5e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1130
Acc16I TGCGCA 1 cut(s) 1721
Acc65I GGTACC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 845
AccI GTMKAC 1 cut(s) 2745
AccII CGCG 2 cut(s) 166, 333
AciI CCGC 8 cut(s) 62, 108, 124, 156, 164, 191, 666, 855
AclWI GGATC 1 cut(s) 2419
AcsI RAATTY 7 cut(s) 318, 467, 1037, 1802, 1862, 1945, 2078
AcuI CTGAAG 5 cut(s) 381, 891, 1427, 1487, 2450
AfaI GTAC 4 cut(s) 81, 847, 1472, 1632
AfiI CCNNNNNNNGG 2 cut(s) 306, 1785
AflIII ACRYGT 2 cut(s) 751, 2484
AjnI CCWGG 3 cut(s) 305, 1316, 2350
AjuI GAANNNNNNNTTGG 2 cut(s) 2869, 2901
AleI CACNNNNGTG 2 cut(s) 75, 2207
AloI GAACNNNNNNTCC 2 cut(s) 2246, 2278
Alw21I GWGCWC 3 cut(s) 2050, 2679, 3106
Alw26I GTCTC 7 cut(s) 341, 1080, 1334, 1514, 2202, 2328, 2347
Alw44I GTGCAC 1 cut(s) 2046
AlwI GGATC 1 cut(s) 2419
AoxI GGCC 5 cut(s) 4, 58, 526, 835, 2912
ApaLI GTGCAC 1 cut(s) 2046
ApoI RAATTY 7 cut(s) 318, 467, 1037, 1802, 1862, 1945, 2078
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 1 cut(s) 2904
Asp718I GGTACC 1 cut(s) 845
AspLEI GCGC 3 cut(s) 107, 168, 1722
AspS9I GGNCC 3 cut(s) 4, 59, 836
AsuHPI GGTGA 7 cut(s) 113, 273, 433, 601, 1768, 2467, 2588
BaeGI GKGCMC 2 cut(s) 26, 2050
BaeI ACNNNNGTAYC 2 cut(s) 261, 294
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 1 cut(s) 2679
BarI GAAGNNNNNNTAC 2 cut(s) 1614, 1646
BauI CACGAG 1 cut(s) 1215
BbsI GAAGAC 2 cut(s) 879, 2969
Bbv12I GWGCWC 3 cut(s) 2050, 2679, 3106
BccI CCATC 6 cut(s) 81, 1604, 2256, 2424, 2699, 3067
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 4 cut(s) 774, 808, 2048, 2082
BciT130I CCWGG 3 cut(s) 307, 1318, 2352
BclI TGATCA 1 cut(s) 1044
BcoDI GTCTC 7 cut(s) 341, 1080, 1334, 1514, 2202, 2328, 2347
BfaI CTAG 2 cut(s) 1562, 2093
BfmI CTRYAG 7 cut(s) 823, 903, 1820, 1910, 2452, 2895, 3107
BglII AGATCT 1 cut(s) 2685
BlpI GCTNAGC 2 cut(s) 1500, 2364
Bme1390I CCNGG 3 cut(s) 307, 1318, 2352
BmgT120I GGNCC 3 cut(s) 4, 59, 836
BmiI GGNNCC 2 cut(s) 93, 847
BmrFI CCNGG 3 cut(s) 307, 1318, 2352
BmrI ACTGGG 1 cut(s) 1002
BmuI ACTGGG 1 cut(s) 1002
BpiI GAAGAC 2 cut(s) 879, 2969
BplI GAGNNNNNCTC 6 cut(s) 1180, 1212, 1632, 1664, 1823, 1855
BpmI CTGGAG 1 cut(s) 289
Bpu1102I GCTNAGC 2 cut(s) 1500, 2364
BpuEI CTTGAG 3 cut(s) 365, 624, 1100
Bsa29I ATCGAT 1 cut(s) 624
BsaAI YACGTR 1 cut(s) 752
BsaBI GATNNNNATC 2 cut(s) 2690, 2987
BsaI GGTCTC 2 cut(s) 1334, 2347
BsaJI CCNNGG 7 cut(s) 201, 1316, 1317, 1766, 1867, 2289, 2350
BsaWI WCCGGW 1 cut(s) 88
BsaXI ACNNNNNCTCC 2 cut(s) 2325, 2355
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 1785
Bse1I ACTGG 4 cut(s) 997, 1097, 1151, 1158
Bse3DI GCAATG 4 cut(s) 317, 1511, 2358, 2389
Bse8I GATNNNNATC 2 cut(s) 2690, 2987
BseBI CCWGG 3 cut(s) 307, 1318, 2352
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 7 cut(s) 201, 1316, 1317, 1766, 1867, 2289, 2350
BseGI GGATG 7 cut(s) 44, 1060, 1270, 2166, 2285, 2607, 3078
BseJI GATNNNNATC 2 cut(s) 2690, 2987
BseLI CCNNNNNNNGG 2 cut(s) 306, 1785
BseMI GCAATG 4 cut(s) 317, 1511, 2358, 2389
BseMII CTCAG 4 cut(s) 1266, 1514, 2056, 2355
BseNI ACTGG 4 cut(s) 997, 1097, 1151, 1158
BseRI GAGGAG 2 cut(s) 2346, 3092
BseSI GKGCMC 2 cut(s) 26, 2050
BseYI CCCAGC 1 cut(s) 1784
Bsh1236I CGCG 2 cut(s) 166, 333
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 5 cut(s) 6, 60, 528, 837, 2914
BshNI GGYRCC 1 cut(s) 845
BshVI ATCGAT 1 cut(s) 624
BsiEI CGRYCG 1 cut(s) 156
BsiHKAI GWGCWC 3 cut(s) 2050, 2679, 3106
BsiSI CCGG 2 cut(s) 89, 146
BslFI GGGAC 3 cut(s) 663, 1872, 2121
BslI CCNNNNNNNGG 2 cut(s) 306, 1785
BsmAI GTCTC 7 cut(s) 341, 1080, 1334, 1514, 2202, 2328, 2347
BsmBI CGTCTC 1 cut(s) 341
BsmFI GGGAC 3 cut(s) 663, 1872, 2121
BsmI GAATGC 2 cut(s) 1399, 1712
BsnI GGCC 5 cut(s) 6, 60, 528, 837, 2914
Bso31I GGTCTC 2 cut(s) 1334, 2347
Bsp1286I GDGCHC 4 cut(s) 26, 2050, 2679, 3106
Bsp143I GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
Bsp1720I GCTNAGC 2 cut(s) 1500, 2364
BspACI CCGC 8 cut(s) 62, 108, 124, 156, 164, 191, 666, 855
BspANI GGCC 5 cut(s) 6, 60, 528, 837, 2914
BspCNI CTCAG 4 cut(s) 1265, 1513, 2055, 2356
BspDI ATCGAT 1 cut(s) 624
BspFNI CGCG 2 cut(s) 166, 333
BspHI TCATGA 1 cut(s) 2265
BspLI GGNNCC 2 cut(s) 93, 847
BspMAI CTGCAG 4 cut(s) 1914, 2456, 2899, 3111
BspPI GGATC 1 cut(s) 2419
BspT107I GGYRCC 1 cut(s) 845
BspTNI GGTCTC 2 cut(s) 1334, 2347
BsrDI GCAATG 4 cut(s) 317, 1511, 2358, 2389
BsrI ACTGG 4 cut(s) 997, 1097, 1151, 1158
BssECI CCNNGG 7 cut(s) 201, 1316, 1317, 1766, 1867, 2289, 2350
BssMI GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
BssSI CACGAG 1 cut(s) 1215
BssT1I CCWWGG 2 cut(s) 201, 2289
Bst2BI CACGAG 1 cut(s) 1215
Bst2UI CCWGG 3 cut(s) 307, 1318, 2352
Bst6I CTCTTC 2 cut(s) 56, 702
BstAPI GCANNNNNTGC 2 cut(s) 1394, 1707
BstBAI YACGTR 1 cut(s) 752
BstC8I GCNNGC 6 cut(s) 62, 769, 1240, 2238, 2402, 2895
BstDEI CTNAG 7 cut(s) 383, 1252, 1500, 1807, 2042, 2364, 2950
BstF5I GGATG 7 cut(s) 44, 1060, 1270, 2166, 2285, 2607, 3078
BstFNI CGCG 2 cut(s) 166, 333
BstHHI GCGC 3 cut(s) 107, 168, 1722
BstKTI GATC 8 cut(s) 979, 1047, 1145, 2125, 2392, 2427, 2688, 2791
BstMAI GTCTC 7 cut(s) 341, 1080, 1334, 1514, 2202, 2328, 2347
BstMBI GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 9 cut(s) 30, 66, 155, 834, 1394, 1707, 2911, 2955, 3002
BstNI CCWGG 3 cut(s) 307, 1318, 2352
BstNSI RCATGY 2 cut(s) 2375, 2488
BstSCI CCNGG 3 cut(s) 305, 1316, 2350
BstSFI CTRYAG 7 cut(s) 823, 903, 1820, 1910, 2452, 2895, 3107
BstSLI GKGCMC 2 cut(s) 26, 2050
BstUI CGCG 2 cut(s) 166, 333
BstV2I GAAGAC 2 cut(s) 879, 2969
BstX2I RGATCY 2 cut(s) 2424, 2685
BstXI CCANNNNNNTGG 1 cut(s) 540
BstYI RGATCY 2 cut(s) 2424, 2685
Bsu15I ATCGAT 1 cut(s) 624
BsuRI GGCC 5 cut(s) 6, 60, 528, 837, 2914
BsuTUI ATCGAT 1 cut(s) 624
BtsCI GGATG 7 cut(s) 44, 1060, 1270, 2166, 2285, 2607, 3078
BtsI GCAGTG 1 cut(s) 3104
BtsIMutI CAGTG 9 cut(s) 481, 990, 1104, 1158, 1165, 1215, 1841, 2050, 3104
Cac8I GCNNGC 6 cut(s) 62, 769, 1240, 2238, 2402, 2895
CciI TCATGA 1 cut(s) 2265
CfoI GCGC 3 cut(s) 107, 168, 1722
Cfr13I GGNCC 3 cut(s) 4, 59, 836
ClaI ATCGAT 1 cut(s) 624
Csp6I GTAC 4 cut(s) 80, 846, 1471, 1631
CviAII CATG 9 cut(s) 285, 1389, 1441, 1533, 1940, 2266, 2372, 2485, 2575
CviQI GTAC 4 cut(s) 80, 846, 1471, 1631
DdeI CTNAG 7 cut(s) 383, 1252, 1500, 1807, 2042, 2364, 2950
DpnI GATC 8 cut(s) 978, 1046, 1144, 2124, 2391, 2426, 2687, 2790
DpnII GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
DraI TTTAAA 2 cut(s) 435, 1861
Eam1104I CTCTTC 2 cut(s) 56, 702
EarI CTCTTC 2 cut(s) 56, 702
Ecl136II GAGCTC 1 cut(s) 2677
Eco130I CCWWGG 2 cut(s) 201, 2289
Eco24I GRGCYC 1 cut(s) 2679
Eco31I GGTCTC 2 cut(s) 1334, 2347
Eco32I GATATC 2 cut(s) 1999, 2989
Eco53kI GAGCTC 1 cut(s) 2677
Eco57I CTGAAG 5 cut(s) 381, 891, 1427, 1487, 2450
EcoICRI GAGCTC 1 cut(s) 2677
EcoRII CCWGG 3 cut(s) 305, 1316, 2350
EcoRV GATATC 2 cut(s) 1999, 2989
EcoT14I CCWWGG 2 cut(s) 201, 2289
EcoT22I ATGCAT 2 cut(s) 1712, 2810
EcoT38I GRGCYC 1 cut(s) 2679
ErhI CCWWGG 2 cut(s) 201, 2289
Esp3I CGTCTC 1 cut(s) 341
FaeI CATG 9 cut(s) 288, 1392, 1444, 1536, 1943, 2269, 2375, 2488, 2578
FalI AAGNNNNNCTT 4 cut(s) 327, 359, 2300, 2332
FaqI GGGAC 3 cut(s) 663, 1872, 2121
FatI CATG 9 cut(s) 284, 1388, 1440, 1532, 1939, 2265, 2371, 2484, 2574
FauI CCCGC 1 cut(s) 69
FauNDI CATATG 3 cut(s) 654, 1737, 3065
FbaI TGATCA 1 cut(s) 1044
FblI GTMKAC 1 cut(s) 2745
FokI GGATG 7 cut(s) 31, 1067, 1277, 2173, 2272, 2594, 3085
FriOI GRGCYC 1 cut(s) 2679
FspBI CTAG 2 cut(s) 1562, 2093
FspI TGCGCA 1 cut(s) 1721
GlaI GCGC 3 cut(s) 106, 167, 1721
GsaI CCCAGC 1 cut(s) 1788
GsuI CTGGAG 1 cut(s) 289
HaeIII GGCC 5 cut(s) 6, 60, 528, 837, 2914
HapII CCGG 2 cut(s) 89, 146
HhaI GCGC 3 cut(s) 107, 168, 1722
Hin1II CATG 9 cut(s) 288, 1392, 1444, 1536, 1943, 2269, 2375, 2488, 2578
Hin6I GCGC 3 cut(s) 105, 166, 1720
HinP1I GCGC 3 cut(s) 105, 166, 1720
HincII GTYRAC 1 cut(s) 2410
HindII GTYRAC 1 cut(s) 2410
HpaI GTTAAC 1 cut(s) 2410
HpaII CCGG 2 cut(s) 89, 146
HphI GGTGA 7 cut(s) 113, 273, 433, 601, 1768, 2467, 2588
Hpy166II GTNNAC 6 cut(s) 80, 296, 367, 2048, 2410, 2746
Hpy8I GTNNAC 6 cut(s) 80, 296, 367, 2048, 2410, 2746
HpyCH4IV ACGT 2 cut(s) 350, 751
HpyF10VI GCNNNNNNNGC 9 cut(s) 30, 66, 155, 834, 1394, 1707, 2911, 2955, 3002
HpyF3I CTNAG 7 cut(s) 383, 1252, 1500, 1807, 2042, 2364, 2950
HpySE526I ACGT 2 cut(s) 350, 751
Hsp92II CATG 9 cut(s) 288, 1392, 1444, 1536, 1943, 2269, 2375, 2488, 2578
HspAI GCGC 3 cut(s) 105, 166, 1720
KpnI GGTACC 1 cut(s) 849
Ksp22I TGATCA 1 cut(s) 1044
KspAI GTTAAC 1 cut(s) 2410
Kzo9I GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
LmnI GCTCC 5 cut(s) 91, 1367, 1664, 2922, 3010
MaeI CTAG 2 cut(s) 1562, 2093
MaeII ACGT 2 cut(s) 350, 751
MaeIII GTNAC 8 cut(s) 235, 268, 543, 724, 898, 2312, 2727, 2766
MalI GATC 8 cut(s) 978, 1046, 1144, 2124, 2391, 2426, 2687, 2790
MboI GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
MflI RGATCY 2 cut(s) 2424, 2685
MhlI GDGCHC 4 cut(s) 26, 2050, 2679, 3106
MlyI GAGTC 3 cut(s) 2288, 2330, 2972
MmeI TCCRAC 1 cut(s) 774
Mph1103I ATGCAT 2 cut(s) 1712, 2810
MroXI GAANNNNTTC 1 cut(s) 2904
MslI CAYNNNNRTG 5 cut(s) 75, 522, 1715, 2207, 2483
MspA1I CMGCKG 1 cut(s) 666
MspI CCGG 2 cut(s) 89, 146
MspR9I CCNGG 3 cut(s) 307, 1318, 2352
Mva1269I GAATGC 2 cut(s) 1399, 1712
MvaI CCWGG 3 cut(s) 307, 1318, 2352
MvnI CGCG 2 cut(s) 166, 333
MwoI GCNNNNNNNGC 9 cut(s) 30, 66, 155, 834, 1394, 1707, 2911, 2955, 3002
NdeI CATATG 3 cut(s) 654, 1737, 3065
NdeII GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
NlaIII CATG 9 cut(s) 288, 1392, 1444, 1536, 1943, 2269, 2375, 2488, 2578
NlaIV GGNNCC 2 cut(s) 93, 847
NmeAIII GCCGAG 1 cut(s) 660
NmuCI GTSAC 2 cut(s) 543, 2727
NsbI TGCGCA 1 cut(s) 1721
NsiI ATGCAT 2 cut(s) 1712, 2810
NspI RCATGY 2 cut(s) 2375, 2488
OliI CACNNNNGTG 2 cut(s) 75, 2207
PagI TCATGA 1 cut(s) 2265
PasI CCCWGGG 1 cut(s) 1317
PciI ACATGT 1 cut(s) 2484
PctI GAATGC 2 cut(s) 1399, 1712
PdmI GAANNNNTTC 1 cut(s) 2904
PfeI GAWTC 8 cut(s) 635, 910, 1093, 1248, 1852, 2494, 2593, 3011
PflFI GACNNNGTC 1 cut(s) 2339
PleI GAGTC 3 cut(s) 2287, 2330, 2972
PpsI GAGTC 3 cut(s) 2287, 2330, 2972
Ppu21I YACGTR 1 cut(s) 752
PscI ACATGT 1 cut(s) 2484
PsiI TTATAA 1 cut(s) 1130
Psp124BI GAGCTC 1 cut(s) 2679
Psp6I CCWGG 3 cut(s) 305, 1316, 2350
PspFI CCCAGC 1 cut(s) 1784
PspGI CCWGG 3 cut(s) 305, 1316, 2350
PspN4I GGNNCC 2 cut(s) 93, 847
PspPI GGNCC 3 cut(s) 4, 59, 836
PstI CTGCAG 4 cut(s) 1914, 2456, 2899, 3111
PsuI RGATCY 2 cut(s) 2424, 2685
PsyI GACNNNGTC 1 cut(s) 2339
RsaI GTAC 4 cut(s) 81, 847, 1472, 1632
RsaNI GTAC 4 cut(s) 80, 846, 1471, 1631
RseI CAYNNNNRTG 5 cut(s) 75, 522, 1715, 2207, 2483
SacI GAGCTC 1 cut(s) 2679
Sau3AI GATC 8 cut(s) 976, 1044, 1142, 2122, 2389, 2424, 2685, 2788
Sau96I GGNCC 3 cut(s) 4, 59, 836
SchI GAGTC 3 cut(s) 2288, 2330, 2972
ScrFI CCNGG 3 cut(s) 307, 1318, 2352
SduI GDGCHC 4 cut(s) 26, 2050, 2679, 3106
SfcI CTRYAG 7 cut(s) 823, 903, 1820, 1910, 2452, 2895, 3107
SmiMI CAYNNNNRTG 5 cut(s) 75, 522, 1715, 2207, 2483
SmlI CTYRAG 3 cut(s) 344, 639, 1079
SmoI CTYRAG 3 cut(s) 344, 639, 1079
SsiI CCGC 8 cut(s) 62, 108, 124, 156, 164, 191, 666, 855
SspMI CTAG 2 cut(s) 1562, 2093
SstI GAGCTC 1 cut(s) 2679
StyD4I CCNGG 3 cut(s) 305, 1316, 2350
StyI CCWWGG 2 cut(s) 201, 2289
TaiI ACGT 2 cut(s) 353, 754
TaqI TCGA 8 cut(s) 624, 1185, 1566, 1650, 2121, 2523, 2981, 3057
TaqII GACCGA 1 cut(s) 2329
TatI WGTACW 1 cut(s) 1470
TauI GCSGC 4 cut(s) 110, 193, 669, 858
TfiI GAWTC 8 cut(s) 635, 910, 1093, 1248, 1852, 2494, 2593, 3011
TscAI CASTG 9 cut(s) 481, 997, 1104, 1158, 1165, 1215, 1848, 2050, 3111
TseFI GTSAC 2 cut(s) 543, 2727
Tsp45I GTSAC 2 cut(s) 543, 2727
TspGWI ACGGA 3 cut(s) 1380, 1630, 2600
TspRI CASTG 9 cut(s) 481, 997, 1104, 1158, 1165, 1215, 1848, 2050, 3111
Tth111I GACNNNGTC 1 cut(s) 2339
VneI GTGCAC 1 cut(s) 2046
XapI RAATTY 7 cut(s) 318, 467, 1037, 1802, 1862, 1945, 2078
XbaI TCTAGA 1 cut(s) 2092
XceI RCATGY 2 cut(s) 2375, 2488
XcmI CCANNNNNNNNNTGG 1 cut(s) 2485
XmiI GTMKAC 1 cut(s) 2745
XmnI GAANNNNTTC 1 cut(s) 2904
XspI CTAG 2 cut(s) 1562, 2093
Zsp2I ATGCAT 2 cut(s) 1712, 2810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.