Rh5BG050300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002553
Physical Location & Seq
Reverse (-)
3991059 .. 4001997
10939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG050300.1

Sequence Viewer

Length: 816 bp
ATGGTGTACAACCTTCCAAACCTGGAAACAATTTCAGTTGAAAAATGTCAGAAGATGGAGGCACTGATAGAGTGGTCAGAATATCCTGATGATTCACTTCATTCAAAGAACCTAAAGTTCATCCATATCTCTGACTGTGAAAAGTTGGTCTACTTATTCAGAAGTGAGATGCTGAGATTCATGCCAAATCTTCAAGAAATCTCCATCCGACGGCGCCAGTTATCAACTGAAGTGGGAAAGGAGCCATATGATCCGCCTGCTATTCATCCAAGATTACAGGACCGTTTGTTTGTTGATCCACTTAAATTGAAGAGCATTTATAGTAATGTACAAATGGAGGGTAACTCTCAGCAAACCATCTGGAACTTTCCTGAACTGAAAATGCACATCATAACTTCTCTGCCACCCAAGCCAAGGAATACGCCATTGAGTGGCGCGAACCAGTTGCAAAGGAATCGCAGCTTTGAGTCTGTACTAGAGGCTCAGACTAAAAAAAGAGAGTCTATACTGGAGGAAGCACCAATCGCATGTGGTATATTATCAAGAACAGTCGGAAGAAATGATAAATTTGTATACTCTCCTCAACTTTCTCCTCGCGGTCGTGATGTTCAGAGCATTATACAGAGGGGGAATGAGTTCCGTTTTGATTATCCGGCCCCACAGTTTAACCTGGATACTGAGGACTCTGGCAACCCACTCAGGAATTTATTTTCCAGTGTCACCTATTATGGTGCTGCTCTGCTGCACAAGTTGCTGAGCCCATCAGTTCCTCCTCGACGCAAAATGCTGCAGGATCAGGATGAGCATGTTGTCTAG

Protein Analysis

271

Amino Acids

31.38

Weight (kDa)

8.35

Isoelectric Point (pI)

68.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 5 - 71 1.7e-07 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 213
AccB7I CCANNNNNTGG 1 cut(s) 431
AccI GTMKAC 2 cut(s) 150, 573
AccII CGCG 2 cut(s) 437, 597
AciI CCGC 2 cut(s) 254, 597
AclWI GGATC 3 cut(s) 245, 290, 801
AcsI RAATTY 2 cut(s) 566, 703
AcuI CTGAAG 1 cut(s) 249
AcyI GRCGYC 1 cut(s) 214
AfaI GTAC 3 cut(s) 8, 330, 474
AfiI CCNNNNNNNGG 3 cut(s) 210, 414, 431
AgsI TTSAA 4 cut(s) 41, 105, 194, 310
AjnI CCWGG 2 cut(s) 21, 669
AluBI AGCT 1 cut(s) 462
AluI AGCT 1 cut(s) 462
AlwI GGATC 3 cut(s) 245, 290, 801
AoxI GGCC 1 cut(s) 654
ApeKI GCWGC 4 cut(s) 459, 734, 742, 787
ApoI RAATTY 2 cut(s) 566, 703
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 2 cut(s) 216, 437
AspS9I GGNCC 2 cut(s) 280, 655
AsuHPI GGTGA 1 cut(s) 712
AvaII GGWCC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 213
BanII GRGCYC 1 cut(s) 761
BbvI GCAGC 4 cut(s) 471, 721, 729, 774
BccI CCATC 4 cut(s) 49, 212, 365, 769
BceAI ACGGC 1 cut(s) 227
BcgI CGANNNNNNTGC 4 cut(s) 427, 437, 461, 471
BciT130I CCWGG 2 cut(s) 23, 671
BciVI GTATCC 1 cut(s) 667
BfaI CTAG 2 cut(s) 476, 814
BfmI CTRYAG 1 cut(s) 788
BfoI RGCGCY 1 cut(s) 217
BfuI GTATCC 1 cut(s) 667
BisI GCNGC 4 cut(s) 460, 735, 743, 788
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 4 cut(s) 461, 736, 744, 789
Bme1390I CCNGG 2 cut(s) 23, 671
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 280, 655
BmiI GGNNCC 3 cut(s) 215, 243, 657
BmrFI CCNGG 2 cut(s) 23, 671
BmsI GCATC 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 1 cut(s) 530
Bpu1102I GCTNAGC 1 cut(s) 755
BsaHI GRCGYC 1 cut(s) 214
BsaJI CCNNGG 1 cut(s) 413
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 414, 431
Bse1I ACTGG 4 cut(s) 217, 442, 513, 714
BseBI CCWGG 2 cut(s) 23, 671
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 4 cut(s) 120, 204, 265, 805
BseLI CCNNNNNNNGG 3 cut(s) 210, 414, 431
BseMII CTCAG 6 cut(s) 164, 362, 497, 669, 712, 746
BseNI ACTGG 4 cut(s) 217, 442, 513, 714
BseRI GAGGAG 3 cut(s) 570, 582, 762
BseXI GCAGC 4 cut(s) 471, 721, 729, 774
BsgI GTGCAG 1 cut(s) 728
Bsh1236I CGCG 2 cut(s) 437, 597
Bsh1285I CGRYCG 1 cut(s) 601
BshFI GGCC 1 cut(s) 656
BshNI GGYRCC 1 cut(s) 213
BsiEI CGRYCG 1 cut(s) 601
BsiSI CCGG 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 210, 414, 431
BsnI GGCC 1 cut(s) 656
Bsp1286I GDGCHC 1 cut(s) 761
Bsp1407I TGTACA 2 cut(s) 6, 328
Bsp143I GATC 3 cut(s) 250, 295, 793
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 2 cut(s) 254, 597
BspANI GGCC 1 cut(s) 656
BspCNI CTCAG 6 cut(s) 165, 361, 496, 670, 711, 747
BspFNI CGCG 2 cut(s) 437, 597
BspLI GGNNCC 3 cut(s) 215, 243, 657
BspMAI CTGCAG 1 cut(s) 792
BspPI GGATC 3 cut(s) 245, 290, 801
BspQI GCTCTTC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 213
BsrGI TGTACA 2 cut(s) 6, 328
BsrI ACTGG 4 cut(s) 217, 442, 513, 714
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 3 cut(s) 250, 295, 793
BssNAI GTATAC 1 cut(s) 574
BssNI GRCGYC 1 cut(s) 214
BssT1I CCWWGG 1 cut(s) 413
Bst1107I GTATAC 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 23, 671
Bst4CI ACNGT 4 cut(s) 137, 284, 550, 663
Bst6I CTCTTC 1 cut(s) 305
BstACI GRCGYC 1 cut(s) 214
BstAPI GCANNNNNTGC 1 cut(s) 751
BstAUI TGTACA 2 cut(s) 6, 328
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 6 cut(s) 173, 348, 483, 678, 698, 755
BstF5I GGATG 4 cut(s) 120, 204, 265, 805
BstFNI CGCG 2 cut(s) 437, 597
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 2 cut(s) 216, 437
BstKTI GATC 3 cut(s) 253, 298, 796
BstMBI GATC 3 cut(s) 250, 295, 793
BstMCI CGRYCG 1 cut(s) 601
BstMWI GCNNNNNNNGC 3 cut(s) 409, 524, 751
BstNI CCWGG 2 cut(s) 23, 671
BstNSI RCATGY 2 cut(s) 531, 809
BstSCI CCNGG 2 cut(s) 21, 669
BstSFI CTRYAG 1 cut(s) 788
BstUI CGCG 2 cut(s) 437, 597
BstV1I GCAGC 4 cut(s) 471, 721, 729, 774
BstZ17I GTATAC 1 cut(s) 574
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 1 cut(s) 656
BtsCI GGATG 4 cut(s) 120, 204, 265, 805
BtsIMutI CAGTG 2 cut(s) 62, 721
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 2 cut(s) 216, 437
Cfr13I GGNCC 2 cut(s) 280, 655
CseI GACGC 1 cut(s) 786
Csp6I GTAC 3 cut(s) 7, 329, 473
CspCI CAANNNNNGTGG 4 cut(s) 213, 248, 288, 323
CviAII CATG 3 cut(s) 181, 528, 806
CviJI RGCY 6 cut(s) 244, 412, 462, 482, 656, 759
CviKI_1 RGCY 6 cut(s) 244, 412, 462, 482, 656, 759
CviQI GTAC 3 cut(s) 7, 329, 473
DdeI CTNAG 6 cut(s) 173, 348, 483, 678, 698, 755
DinI GGCGCC 1 cut(s) 215
DpnI GATC 3 cut(s) 252, 297, 795
DpnII GATC 3 cut(s) 250, 295, 793
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
EciI GGCGGA 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 413
Eco24I GRGCYC 1 cut(s) 761
Eco47I GGWCC 1 cut(s) 280
Eco57I CTGAAG 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 21, 669
EcoT14I CCWWGG 1 cut(s) 413
EcoT38I GRGCYC 1 cut(s) 761
EgeI GGCGCC 1 cut(s) 215
EheI GGCGCC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 413
FaeI CATG 3 cut(s) 184, 531, 809
FatI CATG 3 cut(s) 180, 527, 805
FauNDI CATATG 1 cut(s) 247
FblI GTMKAC 2 cut(s) 150, 573
Fnu4HI GCNGC 4 cut(s) 460, 735, 743, 788
FokI GGATG 4 cut(s) 107, 191, 252, 812
FriOI GRGCYC 1 cut(s) 761
Fsp4HI GCNGC 4 cut(s) 460, 735, 743, 788
FspBI CTAG 2 cut(s) 476, 814
GlaI GCGC 2 cut(s) 215, 436
GluI GCNGC 4 cut(s) 460, 735, 743, 788
GsuI CTGGAG 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 1 cut(s) 656
HapII CCGG 1 cut(s) 653
HgaI GACGC 1 cut(s) 786
HhaI GCGC 2 cut(s) 216, 437
Hin1I GRCGYC 1 cut(s) 214
Hin1II CATG 3 cut(s) 184, 531, 809
Hin6I GCGC 2 cut(s) 214, 435
HinP1I GCGC 2 cut(s) 214, 435
HinfI GANTC 6 cut(s) 92, 177, 454, 467, 500, 683
HpaII CCGG 1 cut(s) 653
HphI GGTGA 1 cut(s) 712
Hpy166II GTNNAC 3 cut(s) 7, 151, 574
Hpy188I TCNGA 8 cut(s) 51, 79, 133, 161, 209, 486, 554, 612
Hpy188III TCNNGA 8 cut(s) 86, 194, 361, 371, 543, 602, 700, 797
Hpy8I GTNNAC 3 cut(s) 7, 151, 574
Hpy99I CGWCG 2 cut(s) 213, 780
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 4 cut(s) 137, 284, 550, 663
HpyCH4V TGCA 4 cut(s) 385, 448, 745, 790
HpyF10VI GCNNNNNNNGC 3 cut(s) 409, 524, 751
HpyF3I CTNAG 6 cut(s) 173, 348, 483, 678, 698, 755
Hsp92I GRCGYC 1 cut(s) 214
Hsp92II CATG 3 cut(s) 184, 531, 809
HspAI GCGC 2 cut(s) 214, 435
KasI GGCGCC 1 cut(s) 213
Kzo9I GATC 3 cut(s) 250, 295, 793
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 4 cut(s) 471, 721, 729, 774
LweI GCATC 1 cut(s) 159
MaeI CTAG 2 cut(s) 476, 814
MaeIII GTNAC 2 cut(s) 341, 718
MalI GATC 3 cut(s) 252, 297, 795
MboI GATC 3 cut(s) 250, 295, 793
MboII GAAGA 4 cut(s) 64, 182, 322, 567
MhlI GDGCHC 1 cut(s) 761
MluCI AATT 4 cut(s) 30, 305, 566, 703
Mly113I GGCGCC 1 cut(s) 214
MlyI GAGTC 3 cut(s) 476, 509, 677
MmeI TCCRAC 2 cut(s) 232, 532
MroXI GAANNNNTTC 1 cut(s) 635
MseI TTAA 2 cut(s) 303, 666
MspI CCGG 1 cut(s) 653
MspR9I CCNGG 2 cut(s) 23, 671
MvaI CCWGG 2 cut(s) 23, 671
MvnI CGCG 2 cut(s) 437, 597
MwoI GCNNNNNNNGC 3 cut(s) 409, 524, 751
NarI GGCGCC 1 cut(s) 214
NdeI CATATG 1 cut(s) 247
NdeII GATC 3 cut(s) 250, 295, 793
NlaIII CATG 3 cut(s) 184, 531, 809
NlaIV GGNNCC 3 cut(s) 215, 243, 657
NmuCI GTSAC 1 cut(s) 718
NspI RCATGY 2 cut(s) 531, 809
PciSI GCTCTTC 1 cut(s) 305
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 3 cut(s) 92, 177, 454
PflMI CCANNNNNTGG 1 cut(s) 431
PkrI GCNGC 4 cut(s) 461, 736, 744, 789
PleI GAGTC 3 cut(s) 475, 508, 677
PluTI GGCGCC 1 cut(s) 217
PpsI GAGTC 3 cut(s) 475, 508, 677
Psp6I CCWGG 2 cut(s) 21, 669
PspGI CCWGG 2 cut(s) 21, 669
PspN4I GGNNCC 3 cut(s) 215, 243, 657
PspPI GGNCC 2 cut(s) 280, 655
PstI CTGCAG 1 cut(s) 792
RsaI GTAC 3 cut(s) 8, 330, 474
RsaNI GTAC 3 cut(s) 7, 329, 473
SapI GCTCTTC 1 cut(s) 305
SaqAI TTAA 2 cut(s) 303, 666
SatI GCNGC 4 cut(s) 460, 735, 743, 788
Sau3AI GATC 3 cut(s) 250, 295, 793
Sau96I GGNCC 2 cut(s) 280, 655
SchI GAGTC 3 cut(s) 476, 509, 677
ScrFI CCNGG 2 cut(s) 23, 671
SduI GDGCHC 1 cut(s) 761
SetI ASST 6 cut(s) 15, 24, 114, 464, 672, 725
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 1 cut(s) 788
SfoI GGCGCC 1 cut(s) 215
SinI GGWCC 1 cut(s) 280
Sse9I AATT 4 cut(s) 30, 305, 566, 703
SsiI CCGC 2 cut(s) 254, 597
SspDI GGCGCC 1 cut(s) 213
SspMI CTAG 2 cut(s) 476, 814
StyD4I CCNGG 2 cut(s) 21, 669
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 4 cut(s) 137, 284, 550, 663
TaqI TCGA 1 cut(s) 775
TasI AATT 4 cut(s) 30, 305, 566, 703
TatI WGTACW 3 cut(s) 6, 328, 472
TfiI GAWTC 3 cut(s) 92, 177, 454
Tru1I TTAA 2 cut(s) 303, 666
Tru9I TTAA 2 cut(s) 303, 666
TscAI CASTG 2 cut(s) 69, 721
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 4 cut(s) 459, 734, 742, 787
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 4 cut(s) 89, 109, 169, 254
TspGWI ACGGA 1 cut(s) 629
TspRI CASTG 2 cut(s) 69, 721
Van91I CCANNNNNTGG 1 cut(s) 431
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 2 cut(s) 566, 703
XceI RCATGY 2 cut(s) 531, 809
XmiI GTMKAC 2 cut(s) 150, 573
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 2 cut(s) 476, 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.