Rmu_sc0000078.1_g000039

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000078.1
OG0002248
Physical Location & Seq
Reverse (-)
166581 .. 170835
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1308 bp
atggcccctgacgcttctgatgcacttgcagctagggaaatagtgcagcagtttctgaatgctgcatgtactggggatgttgatctcttaaagagcgacactccccttattcatgctgctcgcctaggccatactgatactgcaaaactgcttcttgagtatggtgccaatccgtctatagccagtgatttaggggctacagctcttcatcattccgctggactaggaaacattgagttgctgaggtacttaatttccaagggtgtcgatgtcaattcacaaagtgatgctggttctcctttaatttgggctgctggtcatgaccaaccagatgctgtgaaagttctgttagaacaccacgctaatcctaatgctgaaagtgatgatgatattactccattgttgtcatctgtggcagctggttcattggcatgcttggagttattgattcaggcaggtgccaaagtaaatatgagtgctggtggagcgactcctttacacattgctgctgatattgggaacttggagattataaaatgtttgttaaaagctggagctgatcccaatgtaactgatgaggagggtttgaagccaatacaggttgcagctgcaagtgggaaccgaagcgctgtggagatcctctttcccttgacatcacaagttgaaaccatttcaaaatggacagttgacggcatagttgagtatatgcagtctgaaacaagcagacaacaggaacaaccgcgaaatactaaggaacttaatgtcccaaaagactctacttcactgaagaaagagctccctgaggtgacacctgaggcaaagaaaagagctgcagaagcaaaattgagaggaggcgatgctttcagtagaaaggattatcaagcggctgttgatgcatacacgcaggcaattgatttggacccaactgatgccatttttctttccaatcgaagcctttgttggcttcgtcttggccaagctgaacatgccttagctgatgcaaaggcctgcagggaattgaaaccagattggccaaaggcttgctttcgagaaggtgcagcattgcgcctaatgcaggcacgtacacactttcccatttactttctcatgttgtttactgcacaaaagaagaccaagagccatgaaatgttttgtttattattgcagaagtttgatgaagcagccaattgtttttatgagggcgtgcagctcgaccctgaaagtaaggaactcgtcgatgctttcagggaagcggttgaagctggaaggcagttccacagtaaagataaacaaaaaccaaaaccttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5 DUF1685 TPR_17 TPR_8

Protein Analysis

435

Amino Acids

47.13

Weight (kDa)

5.38

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 12 - 94 1.2e-14 Ankyrin repeats (3 copies)
Ank PF00023 33 - 61 5.4e-06 Ankyrin repeat
Ank_4 PF13637 37 - 85 4.9e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 98 - 158 7e-08 Ankyrin repeats (3 copies)
Ank_4 PF13637 140 - 182 5.6e-07 Ankyrin repeats (many copies)
Ank_5 PF13857 149 - 202 1.3e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 162 - 216 2.7e-09 Ankyrin repeats (3 copies)
Ank PF00023 162 - 192 3.4e-09 Ankyrin repeat
Ank_3 PF13606 162 - 190 3.5e-06 Ankyrin repeat
Ank_4 PF13637 173 - 214 5.4e-06 Ankyrin repeats (many copies)
TPR_BSK1_C PF25575 274 - 349 6.3e-13 Serine/threonine-protein kinase BSK1-like, TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
AarI CACCTGC 1 cut(s) 446
Acc36I ACCTGC 1 cut(s) 446
AccB1I GGYRCC 2 cut(s) 164, 458
AccII CGCG 1 cut(s) 742
AciI CCGC 4 cut(s) 216, 740, 884, 1253
AclWI GGATC 2 cut(s) 554, 631
AcoI YGGCCR 2 cut(s) 973, 1031
AcuI CTGAAG 1 cut(s) 806
AdeI CACNNNGTG 1 cut(s) 284
AfaI GTAC 3 cut(s) 70, 248, 1084
AfeI AGCGCT 1 cut(s) 628
AfiI CCNNNNNNNGG 1 cut(s) 1075
AgsI TTSAA 5 cut(s) 589, 665, 675, 1021, 1259
AjuI GAANNNNNNNTTGG 2 cut(s) 943, 975
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
Alw21I GWGCWC 1 cut(s) 798
AlwI GGATC 2 cut(s) 554, 631
AlwNI CAGNNNCTG 2 cut(s) 55, 335
Aor51HI AGCGCT 1 cut(s) 628
AoxI GGCC 5 cut(s) 3, 127, 973, 1005, 1031
AspA2I CCTAGG 1 cut(s) 124
AspLEI GCGC 2 cut(s) 629, 1068
AspS9I GGNCC 2 cut(s) 4, 919
AsuHPI GGTGA 1 cut(s) 817
AvaII GGWCC 1 cut(s) 919
AvrII CCTAGG 1 cut(s) 124
AxyI CCTNAGG 2 cut(s) 801, 813
BalI TGGCCA 2 cut(s) 975, 1033
BanI GGYRCC 2 cut(s) 164, 458
BanII GRGCYC 1 cut(s) 798
BbsI GAAGAC 1 cut(s) 1136
Bbv12I GWGCWC 1 cut(s) 798
BbvCI CCTCAGC 1 cut(s) 242
BceAI ACGGC 1 cut(s) 706
BfaI CTAG 3 cut(s) 33, 125, 224
BfmI CTRYAG 4 cut(s) 177, 198, 831, 1009
BfoI RGCGCY 1 cut(s) 630
BfuAI ACCTGC 1 cut(s) 446
BlnI CCTAGG 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 919
BmgT120I GGNCC 2 cut(s) 4, 919
BmiI GGNNCC 5 cut(s) 6, 166, 460, 620, 921
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 8 cut(s) 10, 277, 322, 847, 883, 919, 988, 1228
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 1136
BplI GAGNNNNNCTC 2 cut(s) 85, 117
BpmI CTGGAG 1 cut(s) 573
Bpu10I CCTNAGC 2 cut(s) 242, 991
BpuEI CTTGAG 1 cut(s) 176
BsaAI YACGTR 1 cut(s) 1082
BsaBI GATNNNNATC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 124, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 1075
Bse1I ACTGG 2 cut(s) 76, 183
Bse21I CCTNAGG 2 cut(s) 801, 813
Bse3DI GCAATG 2 cut(s) 501, 1061
Bse8I GATNNNNATC 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 124, 258
BseGI GGATG 1 cut(s) 82
BseJI GATNNNNATC 1 cut(s) 81
BseLI CCNNNNNNNGG 1 cut(s) 1075
BseMI GCAATG 2 cut(s) 501, 1061
BseMII CTCAG 3 cut(s) 233, 792, 804
BseNI ACTGG 2 cut(s) 76, 183
BseRI GAGGAG 2 cut(s) 593, 864
BsgI GTGCAG 4 cut(s) 65, 1077, 1104, 1226
Bsh1236I CGCG 1 cut(s) 742
BshFI GGCC 5 cut(s) 5, 129, 975, 1007, 1033
BshNI GGYRCC 2 cut(s) 164, 458
BsiHKAI GWGCWC 1 cut(s) 798
BslFI GGGAC 1 cut(s) 749
BslI CCNNNNNNNGG 1 cut(s) 1075
BsmFI GGGAC 1 cut(s) 749
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 5 cut(s) 5, 129, 975, 1007, 1033
Bsp1286I GDGCHC 1 cut(s) 798
Bsp143I GATC 3 cut(s) 82, 559, 636
BspACI CCGC 4 cut(s) 216, 740, 884, 1253
BspANI GGCC 5 cut(s) 5, 129, 975, 1007, 1033
BspCNI CTCAG 3 cut(s) 234, 793, 805
BspFNI CGCG 1 cut(s) 742
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 5 cut(s) 6, 166, 460, 620, 921
BspMAI CTGCAG 2 cut(s) 835, 1013
BspMI ACCTGC 1 cut(s) 446
BspPI GGATC 2 cut(s) 554, 631
BspQI GCTCTTC 1 cut(s) 210
BspT107I GGYRCC 2 cut(s) 164, 458
BsrDI GCAATG 2 cut(s) 501, 1061
BsrI ACTGG 2 cut(s) 76, 183
BssECI CCNNGG 2 cut(s) 124, 258
BssMI GATC 3 cut(s) 82, 559, 636
BssT1I CCWWGG 2 cut(s) 124, 258
Bst4CI ACNGT 2 cut(s) 685, 1280
Bst6I CTCTTC 1 cut(s) 210
BstBAI YACGTR 1 cut(s) 1082
BstC8I GCNNGC 7 cut(s) 121, 433, 906, 1009, 1042, 1077, 1205
BstDEI CTNAG 6 cut(s) 242, 750, 801, 813, 991, 1305
BstENI CCTNNNNNAGG 1 cut(s) 1073
BstF5I GGATG 1 cut(s) 82
BstFNI CGCG 1 cut(s) 742
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 2 cut(s) 629, 1068
BstKTI GATC 3 cut(s) 85, 562, 639
BstMBI GATC 3 cut(s) 82, 559, 636
BstMWI GCNNNNNNNGC 9 cut(s) 11, 20, 29, 485, 836, 893, 986, 1072, 1259
BstNSI RCATGY 3 cut(s) 69, 435, 989
BstSFI CTRYAG 4 cut(s) 177, 198, 831, 1009
BstUI CGCG 1 cut(s) 742
BstV2I GAAGAC 1 cut(s) 1136
BstX2I RGATCY 1 cut(s) 636
BstYI RGATCY 1 cut(s) 636
Bsu36I CCTNAGG 2 cut(s) 801, 813
BsuRI GGCC 5 cut(s) 5, 129, 975, 1007, 1033
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 1 cut(s) 82
BtsIMutI CAGTG 2 cut(s) 190, 782
BveI ACCTGC 1 cut(s) 446
Cac8I GCNNGC 7 cut(s) 121, 433, 906, 1009, 1042, 1077, 1205
CaiI CAGNNNCTG 2 cut(s) 55, 335
CciI TCATGA 1 cut(s) 319
CfoI GCGC 2 cut(s) 629, 1068
Cfr13I GGNCC 2 cut(s) 4, 919
CseI GACGC 1 cut(s) 20
Csp6I GTAC 3 cut(s) 69, 247, 1083
CviAII CATG 7 cut(s) 66, 113, 320, 432, 986, 1108, 1142
CviQI GTAC 3 cut(s) 69, 247, 1083
DdeI CTNAG 6 cut(s) 242, 750, 801, 813, 991, 1305
DpnI GATC 3 cut(s) 84, 561, 638
DpnII GATC 3 cut(s) 82, 559, 636
DraIII CACNNNGTG 1 cut(s) 284
EaeI YGGCCR 2 cut(s) 973, 1031
Eam1104I CTCTTC 1 cut(s) 210
EarI CTCTTC 1 cut(s) 210
Ecl136II GAGCTC 1 cut(s) 796
Eco130I CCWWGG 2 cut(s) 124, 258
Eco147I AGGCCT 1 cut(s) 1007
Eco24I GRGCYC 1 cut(s) 798
Eco47I GGWCC 1 cut(s) 919
Eco47III AGCGCT 1 cut(s) 628
Eco53kI GAGCTC 1 cut(s) 796
Eco57I CTGAAG 1 cut(s) 806
Eco81I CCTNAGG 2 cut(s) 801, 813
EcoICRI GAGCTC 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 1073
EcoT14I CCWWGG 2 cut(s) 124, 258
EcoT22I ATGCAT 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 798
ErhI CCWWGG 2 cut(s) 124, 258
FaeI CATG 7 cut(s) 69, 116, 323, 435, 989, 1111, 1145
FalI AAGNNNNNCTT 2 cut(s) 1028, 1060
FaqI GGGAC 1 cut(s) 749
FatI CATG 7 cut(s) 65, 112, 319, 431, 985, 1107, 1141
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 1 cut(s) 798
FspBI CTAG 3 cut(s) 33, 125, 224
GlaI GCGC 2 cut(s) 628, 1067
GsuI CTGGAG 1 cut(s) 573
HaeII RGCGCY 1 cut(s) 630
HaeIII GGCC 5 cut(s) 5, 129, 975, 1007, 1033
HgaI GACGC 1 cut(s) 20
HhaI GCGC 2 cut(s) 629, 1068
Hin1II CATG 7 cut(s) 69, 116, 323, 435, 989, 1111, 1145
Hin6I GCGC 2 cut(s) 627, 1066
HinP1I GCGC 2 cut(s) 627, 1066
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HinfI GANTC 3 cut(s) 448, 490, 773
HphI GGTGA 1 cut(s) 817
Hpy166II GTNNAC 3 cut(s) 688, 1085, 1116
Hpy188I TCNGA 3 cut(s) 19, 57, 715
Hpy188III TCNNGA 3 cut(s) 155, 320, 1049
Hpy8I GTNNAC 3 cut(s) 688, 1085, 1116
Hpy99I CGWCG 1 cut(s) 1238
HpyAV CCTTC 2 cut(s) 1046, 1260
HpyCH4III ACNGT 2 cut(s) 685, 1280
HpyCH4IV ACGT 1 cut(s) 1081
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 20, 29, 485, 836, 893, 986, 1072, 1259
HpyF3I CTNAG 6 cut(s) 242, 750, 801, 813, 991, 1305
HpySE526I ACGT 1 cut(s) 1081
Hsp92II CATG 7 cut(s) 69, 116, 323, 435, 989, 1111, 1145
HspAI GCGC 2 cut(s) 627, 1066
Kzo9I GATC 3 cut(s) 82, 559, 636
LguI GCTCTTC 1 cut(s) 210
LmnI GCTCC 3 cut(s) 485, 554, 801
LweI GCATC 8 cut(s) 10, 277, 322, 847, 883, 919, 988, 1228
MaeI CTAG 3 cut(s) 33, 125, 224
MaeII ACGT 1 cut(s) 1081
MaeIII GTNAC 2 cut(s) 568, 805
MalI GATC 3 cut(s) 84, 561, 638
MboI GATC 3 cut(s) 82, 559, 636
MboII GAAGA 3 cut(s) 197, 799, 1141
MfeI CAATTG 2 cut(s) 909, 1186
MflI RGATCY 1 cut(s) 636
MhlI GDGCHC 1 cut(s) 798
MlsI TGGCCA 2 cut(s) 975, 1033
MluCI AATT 7 cut(s) 252, 274, 303, 842, 909, 1016, 1186
MluNI TGGCCA 2 cut(s) 975, 1033
MlyI GAGTC 2 cut(s) 484, 767
MnlI CCTC 9 cut(s) 237, 571, 574, 650, 796, 808, 842, 845, 1192
Mox20I TGGCCA 2 cut(s) 975, 1033
Mph1103I ATGCAT 1 cut(s) 898
MscI TGGCCA 2 cut(s) 975, 1033
MseI TTAA 5 cut(s) 89, 251, 302, 545, 759
MslI CAYNNNNRTG 1 cut(s) 430
Msp20I TGGCCA 2 cut(s) 975, 1033
MspA1I CMGCKG 3 cut(s) 218, 419, 608
MunI CAATTG 2 cut(s) 909, 1186
Mva1269I GAATGC 1 cut(s) 64
MvnI CGCG 1 cut(s) 742
MwoI GCNNNNNNNGC 9 cut(s) 11, 20, 29, 485, 836, 893, 986, 1072, 1259
NdeII GATC 3 cut(s) 82, 559, 636
NlaIII CATG 7 cut(s) 69, 116, 323, 435, 989, 1111, 1145
NlaIV GGNNCC 5 cut(s) 6, 166, 460, 620, 921
NmuCI GTSAC 1 cut(s) 805
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 3 cut(s) 69, 435, 989
PaeI GCATGC 1 cut(s) 435
PagI TCATGA 1 cut(s) 319
PaqCI CACCTGC 1 cut(s) 446
PceI AGGCCT 1 cut(s) 1007
PciSI GCTCTTC 1 cut(s) 210
PctI GAATGC 1 cut(s) 64
PfeI GAWTC 1 cut(s) 448
PleI GAGTC 2 cut(s) 484, 767
PpsI GAGTC 2 cut(s) 484, 767
Ppu21I YACGTR 1 cut(s) 1082
PsiI TTATAA 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 798
PspN4I GGNNCC 5 cut(s) 6, 166, 460, 620, 921
PspPI GGNCC 2 cut(s) 4, 919
PstI CTGCAG 2 cut(s) 835, 1013
PstNI CAGNNNCTG 2 cut(s) 55, 335
PsuI RGATCY 1 cut(s) 636
PvuII CAGCTG 2 cut(s) 419, 608
RsaI GTAC 3 cut(s) 70, 248, 1084
RsaNI GTAC 3 cut(s) 69, 247, 1083
RseI CAYNNNNRTG 1 cut(s) 430
SacI GAGCTC 1 cut(s) 798
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 5 cut(s) 89, 251, 302, 545, 759
Sau3AI GATC 3 cut(s) 82, 559, 636
Sau96I GGNCC 2 cut(s) 4, 919
SbfI CCTGCAGG 1 cut(s) 1013
SchI GAGTC 2 cut(s) 484, 767
SdaI CCTGCAGG 1 cut(s) 1013
SduI GDGCHC 1 cut(s) 798
SfaNI GCATC 8 cut(s) 10, 277, 322, 847, 883, 919, 988, 1228
SfcI CTRYAG 4 cut(s) 177, 198, 831, 1009
SinI GGWCC 1 cut(s) 919
SmiMI CAYNNNNRTG 1 cut(s) 430
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SphI GCATGC 1 cut(s) 435
Sse8387I CCTGCAGG 1 cut(s) 1013
Sse9I AATT 7 cut(s) 252, 274, 303, 842, 909, 1016, 1186
SseBI AGGCCT 1 cut(s) 1007
SsiI CCGC 4 cut(s) 216, 740, 884, 1253
SspMI CTAG 3 cut(s) 33, 125, 224
SstI GAGCTC 1 cut(s) 798
StuI AGGCCT 1 cut(s) 1007
StyI CCWWGG 2 cut(s) 124, 258
TaaI ACNGT 2 cut(s) 685, 1280
TaiI ACGT 1 cut(s) 1084
TaqI TCGA 5 cut(s) 267, 949, 1048, 1212, 1236
TasI AATT 7 cut(s) 252, 274, 303, 842, 909, 1016, 1186
TatI WGTACW 1 cut(s) 68
TauI GCSGC 1 cut(s) 887
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 5 cut(s) 89, 251, 302, 545, 759
Tru9I TTAA 5 cut(s) 89, 251, 302, 545, 759
TscAI CASTG 2 cut(s) 190, 789
TseFI GTSAC 1 cut(s) 805
Tsp45I GTSAC 1 cut(s) 805
TspDTI ATGAA 5 cut(s) 101, 197, 414, 1158, 1191
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 2 cut(s) 190, 789
VpaK11BI GGWCC 1 cut(s) 919
XagI CCTNNNNNAGG 1 cut(s) 1073
XceI RCATGY 3 cut(s) 69, 435, 989
XmaJI CCTAGG 1 cut(s) 124
XspI CTAG 3 cut(s) 33, 125, 224
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.