Rmu_sc0001972.1_g000021

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001972.1
OG0002553
Physical Location & Seq
Forward (+)
87524 .. 89218
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1227 bp
atgctcatgttgcatgtccatgcaaccgacgctaacgttgctgaggcgaacgaggatgtggatgcgactgagtcccccatagaagcggtgaaagcagatgaatttatcaatggctttgaaccggagcttgaagcagtcagaataagcaagaattgctgtggcctaccatactataggtcacgcggcacaaaactactcggcgaggcagcaagaagtatggttaaagagattaaggagtatgtaacaggaaaggaagtcgggactatacgtgtttatggaatggctggtttcggcaagacaacagttgtgtcggaagttaatgacctcatagtgaatgactacagaagttgtgccccagaaggtacaaatagcggcagtagatacactgaaaaccattttgacaaaattatatgggttactatagagcatcgaggatcaactcaatcatctattgacaacttgcagaataaaattgcagagaaattacatattgatttgtgcataaagccactgggagtagagcgcgcagatacatttgaaagtgcattgaaggagttaagatttttgatcattctggatgatatgcggaagaagctgtctcttgagctaattgggattctaccaacgagggaaaatggctgcaaggttataatagtatctagatcacttccagtgttcagtgacatcaaaattgataagaaggttgagatcaaacctctttcagatacagcggcacgagaattgtttgagagggaagctggcattagattctcagacttgagggatgatactcaaactatggcagagaaaatggtagatgactgtgatagtttgcccttggctattggctttctggctcggatcatgaaagaattaaagagtgatgctgctgagagtgtcgatgctgcacggaatgcagcccttttgaagttacagagttctcacttggaaagcatgaatgaaaaggcattcgctcgtctaaagtacagctatgaaatattgaagaaagatgctccgcaatgtctcctgtattgtgccttgtacccatgtggtcattacattgttagtcaaggtggcagggaatgcataaagatgcgcagattggttaagcatatggcagtccagactaccaaagcaagtcctcagcttttcaccgaagctgggcatcagaaagaaaaattcctaggggattggctacacataacagctgtcacctttgatgcctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_4 LRR_8 NB-ARC

Protein Analysis

408

Amino Acids

45.67

Weight (kDa)

6.0

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 128 - 253 1.5e-14 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 650
Acc16I TGCGCA 1 cut(s) 1097
AccII CGCG 2 cut(s) 183, 525
AciI CCGC 6 cut(s) 86, 183, 372, 586, 731, 1016
AclI AACGTT 1 cut(s) 36
AclWI GGATC 2 cut(s) 442, 869
AcsI RAATTY 2 cut(s) 101, 1178
AfaI GTAC 3 cut(s) 364, 986, 1043
AfiI CCNNNNNNNGG 1 cut(s) 1161
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 6 cut(s) 119, 131, 539, 550, 928, 1003
AluBI AGCT 8 cut(s) 127, 595, 607, 758, 990, 1147, 1160, 1208
AluI AGCT 8 cut(s) 127, 595, 607, 758, 990, 1147, 1160, 1208
Alw26I GTCTC 2 cut(s) 603, 1028
AlwI GGATC 2 cut(s) 442, 869
AoxI GGCC 1 cut(s) 160
ApeKI GCWGC 5 cut(s) 206, 639, 887, 905, 917
ApoI RAATTY 2 cut(s) 101, 1178
AspA2I CCTAGG 1 cut(s) 1183
AspLEI GCGC 3 cut(s) 525, 527, 1098
AsuHPI GGTGA 3 cut(s) 100, 1144, 1204
AvrII CCTAGG 1 cut(s) 1183
BaeGI GKGCMC 1 cut(s) 355
BauI CACGAG 1 cut(s) 735
BbvCI CCTCAGC 2 cut(s) 42, 1143
BbvI GCAGC 5 cut(s) 218, 626, 874, 892, 929
BclI TGATCA 1 cut(s) 567
BcoDI GTCTC 2 cut(s) 603, 1028
BfaI CTAG 3 cut(s) 660, 1184, 1225
BfmI CTRYAG 3 cut(s) 172, 340, 420
BisI GCNGC 8 cut(s) 184, 207, 373, 640, 732, 888, 906, 918
BlnI CCTAGG 1 cut(s) 1183
BlsI GCNGC 8 cut(s) 185, 208, 374, 641, 733, 889, 907, 919
BmrI ACTGGG 1 cut(s) 521
BmsI GCATC 8 cut(s) 52, 436, 874, 892, 1000, 1083, 1174, 1210
BmuI ACTGGG 1 cut(s) 521
Bpu10I CCTNAGC 2 cut(s) 42, 1143
BpuEI CTTGAG 2 cut(s) 623, 799
BsaAI YACGTR 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 837, 1183
BsaWI WCCGGW 1 cut(s) 121
Bsc4I CCNNNNNNNGG 1 cut(s) 1161
Bse1I ACTGG 2 cut(s) 516, 671
Bse3DI GCAATG 1 cut(s) 1025
BseDI CCNNGG 2 cut(s) 837, 1183
BseGI GGATG 4 cut(s) 61, 67, 583, 790
BseLI CCNNNNNNNGG 1 cut(s) 1161
BseMI GCAATG 1 cut(s) 1025
BseMII CTCAG 5 cut(s) 33, 60, 786, 882, 1157
BseNI ACTGG 2 cut(s) 516, 671
BsePI GCGCGC 1 cut(s) 523
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 5 cut(s) 218, 626, 874, 892, 929
BseYI CCCAGC 1 cut(s) 1160
BsgI GTGCAG 1 cut(s) 891
Bsh1236I CGCG 2 cut(s) 183, 525
BshFI GGCC 1 cut(s) 162
BsiSI CCGG 1 cut(s) 122
BslFI GGGAC 2 cut(s) 58, 274
BslI CCNNNNNNNGG 1 cut(s) 1161
BsmAI GTCTC 2 cut(s) 603, 1028
BsmFI GGGAC 2 cut(s) 58, 274
BsmI GAATGC 3 cut(s) 919, 968, 1088
BsnI GGCC 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 5 cut(s) 434, 567, 662, 708, 861
BspACI CCGC 6 cut(s) 86, 183, 372, 586, 731, 1016
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 5 cut(s) 34, 61, 785, 883, 1156
BspFNI CGCG 2 cut(s) 183, 525
BspHI TCATGA 1 cut(s) 864
BspPI GGATC 2 cut(s) 442, 869
BsrDI GCAATG 1 cut(s) 1025
BsrI ACTGG 2 cut(s) 516, 671
BssECI CCNNGG 2 cut(s) 837, 1183
BssHII GCGCGC 1 cut(s) 523
BssMI GATC 5 cut(s) 434, 567, 662, 708, 861
BssSI CACGAG 1 cut(s) 735
BssT1I CCWWGG 2 cut(s) 837, 1183
Bst2BI CACGAG 1 cut(s) 735
Bst4CI ACNGT 2 cut(s) 304, 824
BstAPI GCANNNNNTGC 3 cut(s) 153, 914, 1083
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 2 cut(s) 525, 760
BstDEI CTNAG 5 cut(s) 42, 69, 772, 891, 1143
BstF5I GGATG 4 cut(s) 61, 67, 583, 790
BstFNI CGCG 2 cut(s) 183, 525
BstHHI GCGC 3 cut(s) 525, 527, 1098
BstKTI GATC 5 cut(s) 437, 570, 665, 711, 864
BstMAI GTCTC 2 cut(s) 603, 1028
BstMBI GATC 5 cut(s) 434, 567, 662, 708, 861
BstMWI GCNNNNNNNGC 8 cut(s) 10, 29, 38, 92, 153, 592, 914, 1083
BstNSI RCATGY 1 cut(s) 17
BstSFI CTRYAG 3 cut(s) 172, 340, 420
BstSLI GKGCMC 1 cut(s) 355
BstUI CGCG 2 cut(s) 183, 525
BstV1I GCAGC 5 cut(s) 218, 626, 874, 892, 929
BsuRI GGCC 1 cut(s) 162
BtsCI GGATG 4 cut(s) 61, 67, 583, 790
BtsIMutI CAGTG 4 cut(s) 384, 509, 678, 685
Cac8I GCNNGC 2 cut(s) 525, 760
CciI TCATGA 1 cut(s) 864
CfoI GCGC 3 cut(s) 525, 527, 1098
CseI GACGC 1 cut(s) 38
Csp6I GTAC 3 cut(s) 363, 985, 1042
CviAII CATG 6 cut(s) 7, 14, 20, 865, 955, 1047
CviQI GTAC 3 cut(s) 363, 985, 1042
DdeI CTNAG 5 cut(s) 42, 69, 772, 891, 1143
DpnI GATC 5 cut(s) 436, 569, 664, 710, 863
DpnII GATC 5 cut(s) 434, 567, 662, 708, 861
Eco130I CCWWGG 2 cut(s) 837, 1183
EcoT14I CCWWGG 2 cut(s) 837, 1183
EcoT22I ATGCAT 1 cut(s) 1088
ErhI CCWWGG 2 cut(s) 837, 1183
FaeI CATG 6 cut(s) 10, 17, 23, 868, 958, 1050
FaqI GGGAC 2 cut(s) 58, 274
FatI CATG 6 cut(s) 6, 13, 19, 864, 954, 1046
FauNDI CATATG 1 cut(s) 1113
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 8 cut(s) 184, 207, 373, 640, 732, 888, 906, 918
FokI GGATG 4 cut(s) 68, 74, 590, 797
Fsp4HI GCNGC 8 cut(s) 184, 207, 373, 640, 732, 888, 906, 918
FspBI CTAG 3 cut(s) 660, 1184, 1225
FspI TGCGCA 1 cut(s) 1097
GlaI GCGC 3 cut(s) 524, 526, 1097
GluI GCNGC 8 cut(s) 184, 207, 373, 640, 732, 888, 906, 918
GsaI CCCAGC 1 cut(s) 1164
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 122
HgaI GACGC 1 cut(s) 38
HhaI GCGC 3 cut(s) 525, 527, 1098
Hin1II CATG 6 cut(s) 10, 17, 23, 868, 958, 1050
Hin6I GCGC 3 cut(s) 523, 525, 1096
HinP1I GCGC 3 cut(s) 523, 525, 1096
HinfI GANTC 3 cut(s) 71, 616, 768
HpaII CCGG 1 cut(s) 122
HphI GGTGA 3 cut(s) 100, 1144, 1204
Hpy188I TCNGA 6 cut(s) 140, 313, 724, 775, 861, 1170
Hpy188III TCNNGA 6 cut(s) 259, 575, 602, 660, 865, 1123
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 353, 544, 694
HpyCH4III ACNGT 2 cut(s) 304, 824
HpyCH4IV ACGT 2 cut(s) 36, 268
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 29, 38, 92, 153, 592, 914, 1083
HpyF3I CTNAG 5 cut(s) 42, 69, 772, 891, 1143
HpySE526I ACGT 2 cut(s) 36, 268
Hsp92II CATG 6 cut(s) 10, 17, 23, 868, 958, 1050
HspAI GCGC 3 cut(s) 523, 525, 1096
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 5 cut(s) 434, 567, 662, 708, 861
LmnI GCTCC 2 cut(s) 124, 1018
Lsp1109I GCAGC 5 cut(s) 218, 626, 874, 892, 929
LweI GCATC 8 cut(s) 52, 436, 874, 892, 1000, 1083, 1174, 1210
MaeI CTAG 3 cut(s) 660, 1184, 1225
MaeII ACGT 2 cut(s) 36, 268
MaeIII GTNAC 6 cut(s) 177, 241, 415, 680, 930, 1210
MalI GATC 5 cut(s) 436, 569, 664, 710, 863
MboI GATC 5 cut(s) 434, 567, 662, 708, 861
MboII GAAGA 2 cut(s) 601, 1015
MhlI GDGCHC 1 cut(s) 355
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 1 cut(s) 291
Mph1103I ATGCAT 1 cut(s) 1088
MseI TTAA 6 cut(s) 222, 231, 318, 557, 875, 1107
MslI CAYNNNNRTG 2 cut(s) 18, 1091
MspA1I CMGCKG 2 cut(s) 731, 1208
MspI CCGG 1 cut(s) 122
Mva1269I GAATGC 3 cut(s) 919, 968, 1088
MvnI CGCG 2 cut(s) 183, 525
MwoI GCNNNNNNNGC 8 cut(s) 10, 29, 38, 92, 153, 592, 914, 1083
NdeI CATATG 1 cut(s) 1113
NdeII GATC 5 cut(s) 434, 567, 662, 708, 861
NlaIII CATG 6 cut(s) 10, 17, 23, 868, 958, 1050
NmeAIII GCCGAG 1 cut(s) 177
NmuCI GTSAC 3 cut(s) 177, 680, 1210
NsbI TGCGCA 1 cut(s) 1097
NsiI ATGCAT 1 cut(s) 1088
NspI RCATGY 1 cut(s) 17
PagI TCATGA 1 cut(s) 864
PauI GCGCGC 1 cut(s) 523
PctI GAATGC 3 cut(s) 919, 968, 1088
PfeI GAWTC 2 cut(s) 616, 768
PflFI GACNNNGTC 1 cut(s) 70
PkrI GCNGC 8 cut(s) 185, 208, 374, 641, 733, 889, 907, 919
PleI GAGTC 1 cut(s) 79
PpsI GAGTC 1 cut(s) 79
Ppu21I YACGTR 1 cut(s) 269
PsiI TTATAA 1 cut(s) 650
Psp1406I AACGTT 1 cut(s) 36
PspFI CCCAGC 1 cut(s) 1160
PsyI GACNNNGTC 1 cut(s) 70
PteI GCGCGC 1 cut(s) 523
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 3 cut(s) 364, 986, 1043
RsaNI GTAC 3 cut(s) 363, 985, 1042
RseI CAYNNNNRTG 2 cut(s) 18, 1091
SaqAI TTAA 6 cut(s) 222, 231, 318, 557, 875, 1107
SatI GCNGC 8 cut(s) 184, 207, 373, 640, 732, 888, 906, 918
Sau3AI GATC 5 cut(s) 434, 567, 662, 708, 861
SchI GAGTC 1 cut(s) 80
SduI GDGCHC 1 cut(s) 355
SfaNI GCATC 8 cut(s) 52, 436, 874, 892, 1000, 1083, 1174, 1210
SfcI CTRYAG 3 cut(s) 172, 340, 420
SmiMI CAYNNNNRTG 2 cut(s) 18, 1091
SmlI CTYRAG 2 cut(s) 602, 778
SmoI CTYRAG 2 cut(s) 602, 778
SsiI CCGC 6 cut(s) 86, 183, 372, 586, 731, 1016
SspI AATATT 1 cut(s) 999
SspMI CTAG 3 cut(s) 660, 1184, 1225
StyI CCWWGG 2 cut(s) 837, 1183
TaaI ACNGT 2 cut(s) 304, 824
TaiI ACGT 2 cut(s) 39, 271
TaqI TCGA 2 cut(s) 430, 900
TatI WGTACW 1 cut(s) 984
TauI GCSGC 3 cut(s) 186, 375, 734
TfiI GAWTC 2 cut(s) 616, 768
Tru1I TTAA 6 cut(s) 222, 231, 318, 557, 875, 1107
Tru9I TTAA 6 cut(s) 222, 231, 318, 557, 875, 1107
TscAI CASTG 4 cut(s) 391, 516, 678, 685
TseFI GTSAC 3 cut(s) 177, 680, 1210
TseI GCWGC 5 cut(s) 206, 639, 887, 905, 917
Tsp45I GTSAC 3 cut(s) 177, 680, 1210
TspDTI ATGAA 5 cut(s) 114, 881, 971, 975, 1008
TspGWI ACGGA 1 cut(s) 925
TspRI CASTG 4 cut(s) 391, 516, 678, 685
Tth111I GACNNNGTC 1 cut(s) 70
XapI RAATTY 2 cut(s) 101, 1178
XbaI TCTAGA 1 cut(s) 659
XceI RCATGY 1 cut(s) 17
XmaJI CCTAGG 1 cut(s) 1183
XspI CTAG 3 cut(s) 660, 1184, 1225
Zsp2I ATGCAT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.