Rmu_sc0002360.1_g000013

Carbohydrate esterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002360.1
OG0003321
Physical Location & Seq
Reverse (-)
62067 .. 63723
1657 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
atgaactccgaactcgacccaactcgtaatccattctcttctcgttttcctcgttcaaggcttatgagactttgtctggatgatatgaatgcctctgctcaaaggcgggctttgttaatgcgcggcatgtttactctcttagctcactatcttgacaacgacgacgacgacgaagacgaagatgaagacgaagacgaagaggaggaggacgaggatccccaaaaacaagaaaaacaccgactcgctaaccggaagcggcgaagcactcgccgtcgcggcacaactacacgaagaagtcgaaacgacggcgttccgagccaagcctacgctgggccccactcgctttcgttgacaagcgacgcggagagcccatttaaaccagagaactcgctcatcccaagaacaaaaaccgacacgtcgtttatcctccttccgaaaaacccggaaatggaatcgccggagcagatcttcattctttcggggcagagcaacatggcgggtcggggcggcgtgatccgtgaccacggccacaaccaccaccagcactgggacggcgtcgttccgtcggaggctcagcccgacccttcgatcctccgcctcaacgctcagctccactgggaggcggcgcgtgagcctctccacgccgacatcgacgccaagaaggtgtgcggtttgggtccgggaatgtcgttcgccaacgcggtgagggggcgcgtggaggggaagattgggctggtgccgtgcgctgttggcggcacggccatcaaggagtgggcgcgtggggagcatctgtacgagaacatggtgaagagggcgcgtgagagcgtgaagaacgggggagagatcaaagggttgctgtggtaccagggggagagcgacacgtcgtctcagcatgatgttgatgcgtaccacgagaacatggttaggctcattgagaatgtgcgccatgaccttgctttgccttcacttccaattatccaggtggctatttgttctggtgatgagaagttcttggagaaaattagggaggttcaattggggatgaaggttgagaatgttgtgtgtgtggatgccaagggattggagctgaaggatgatcatcttcacctgactaccaaggcacaagttcagttgggtcagatgcttgctgatgcgtacctcaagcattttggatcagctaattcccatcatcctgccctttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SASA

Protein Analysis

400

Amino Acids

44.91

Weight (kDa)

5.82

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SASA PF03629 154 - 388 6.1e-96 Carbohydrate esterase, sialic acid-specific acetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 861
AccB1I GGYRCC 2 cut(s) 736, 861
AccII CGCG 8 cut(s) 123, 276, 362, 628, 701, 714, 778, 817
AclWI GGATC 5 cut(s) 209, 222, 508, 583, 1180
AcoI YGGCCR 2 cut(s) 526, 759
AcuI CTGAAG 1 cut(s) 1109
AcyI GRCGYC 2 cut(s) 555, 654
AfaI GTAC 4 cut(s) 794, 863, 908, 1157
AfiI CCNNNNNNNGG 4 cut(s) 330, 448, 547, 619
AflIII ACRYGT 2 cut(s) 414, 879
AgsI TTSAA 2 cut(s) 57, 1034
AhdI GACNNNNNGTC 1 cut(s) 883
AjiI CACGTC 2 cut(s) 417, 882
AjnI CCWGG 2 cut(s) 864, 978
AluBI AGCT 4 cut(s) 143, 610, 1087, 1178
AluI AGCT 4 cut(s) 143, 610, 1087, 1178
Alw26I GTCTC 2 cut(s) 61, 891
AlwI GGATC 5 cut(s) 209, 222, 508, 583, 1180
AoxI GGCC 3 cut(s) 332, 526, 759
ApaI GGGCCC 1 cut(s) 336
Asp718I GGTACC 1 cut(s) 861
AspLEI GCGC 7 cut(s) 123, 628, 714, 746, 778, 817, 945
AspS9I GGNCC 3 cut(s) 332, 333, 677
AsuC2I CCSGG 2 cut(s) 443, 681
AsuHPI GGTGA 4 cut(s) 715, 817, 1010, 1097
AvaII GGWCC 1 cut(s) 677
BaeGI GKGCMC 1 cut(s) 336
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 2 cut(s) 736, 861
BanII GRGCYC 2 cut(s) 336, 371
BauI CACGAG 1 cut(s) 911
BbsI GAAGAC 3 cut(s) 180, 192, 198
BccI CCATC 2 cut(s) 770, 1194
BceAI ACGGC 6 cut(s) 255, 322, 541, 568, 724, 774
BciT130I CCWGG 2 cut(s) 866, 980
BclI TGATCA 1 cut(s) 1096
BcnI CCSGG 2 cut(s) 443, 681
BcoDI GTCTC 2 cut(s) 61, 891
BglI GCCNNNNNGGC 1 cut(s) 276
BglII AGATCT 1 cut(s) 465
BisI GCNGC 6 cut(s) 124, 257, 277, 508, 624, 754
BlpI GCTNAGC 2 cut(s) 573, 606
BlsI GCNGC 6 cut(s) 125, 258, 278, 509, 625, 755
Bme1390I CCNGG 4 cut(s) 443, 681, 866, 980
Bme18I GGWCC 1 cut(s) 677
BmeRI GACNNNNNGTC 1 cut(s) 883
BmgBI CACGTC 2 cut(s) 417, 882
BmgT120I GGNCC 3 cut(s) 332, 333, 677
BmiI GGNNCC 6 cut(s) 216, 334, 335, 678, 738, 863
BmrFI CCNGG 4 cut(s) 443, 681, 866, 980
BmrI ACTGGG 2 cut(s) 556, 625
BmsI GCATC 5 cut(s) 796, 892, 1060, 1131, 1141
BmuI ACTGGG 2 cut(s) 556, 625
BpiI GAAGAC 3 cut(s) 180, 192, 198
Bpu1102I GCTNAGC 2 cut(s) 573, 606
BpuEI CTTGAG 1 cut(s) 1145
BpuMI CCSGG 2 cut(s) 443, 681
BsaBI GATNNNNATC 1 cut(s) 1098
BsaHI GRCGYC 2 cut(s) 555, 654
BsaJI CCNNGG 4 cut(s) 523, 865, 1074, 1116
BsaWI WCCGGW 1 cut(s) 249
BsaXI ACNNNNNCTCC 2 cut(s) 776, 806
Bsc4I CCNNNNNNNGG 4 cut(s) 330, 448, 547, 619
Bse1I ACTGG 2 cut(s) 551, 620
Bse8I GATNNNNATC 1 cut(s) 1098
BseBI CCWGG 2 cut(s) 866, 980
BseDI CCNNGG 4 cut(s) 523, 865, 1074, 1116
BseGI GGATG 6 cut(s) 85, 393, 1047, 1075, 1099, 1189
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 4 cut(s) 330, 448, 547, 619
BseMII CTCAG 3 cut(s) 587, 620, 902
BseNI ACTGG 2 cut(s) 551, 620
BseRI GAGGAG 2 cut(s) 215, 218
BseSI GKGCMC 1 cut(s) 336
BseYI CCCAGC 1 cut(s) 329
Bsh1236I CGCG 8 cut(s) 123, 276, 362, 628, 701, 714, 778, 817
BshFI GGCC 3 cut(s) 334, 528, 761
BshNI GGYRCC 2 cut(s) 736, 861
BsiSI CCGG 4 cut(s) 250, 443, 458, 680
BslFI GGGAC 1 cut(s) 563
BslI CCNNNNNNNGG 4 cut(s) 330, 448, 547, 619
BsmAI GTCTC 2 cut(s) 61, 891
BsmBI CGTCTC 1 cut(s) 891
BsmFI GGGAC 1 cut(s) 563
BsmI GAATGC 1 cut(s) 94
BsnI GGCC 3 cut(s) 334, 528, 761
Bsp120I GGGCCC 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 336, 371
Bsp143I GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
Bsp1720I GCTNAGC 2 cut(s) 573, 606
BspANI GGCC 3 cut(s) 334, 528, 761
BspCNI CTCAG 3 cut(s) 586, 619, 901
BspFNI CGCG 8 cut(s) 123, 276, 362, 628, 701, 714, 778, 817
BspLI GGNNCC 6 cut(s) 216, 334, 335, 678, 738, 863
BspPI GGATC 5 cut(s) 209, 222, 508, 583, 1180
BspT107I GGYRCC 2 cut(s) 736, 861
BsrI ACTGG 2 cut(s) 551, 620
BssECI CCNNGG 4 cut(s) 523, 865, 1074, 1116
BssMI GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
BssNI GRCGYC 2 cut(s) 555, 654
BssSI CACGAG 1 cut(s) 911
BssT1I CCWWGG 2 cut(s) 1074, 1116
Bst2BI CACGAG 1 cut(s) 911
Bst2UI CCWGG 2 cut(s) 866, 980
Bst6I CTCTTC 3 cut(s) 43, 192, 803
BstACI GRCGYC 2 cut(s) 555, 654
BstC8I GCNNGC 2 cut(s) 108, 1146
BstDEI CTNAG 4 cut(s) 139, 573, 606, 888
BstDSI CCRYGG 1 cut(s) 523
BstF5I GGATG 6 cut(s) 85, 393, 1047, 1075, 1099, 1189
BstFNI CGCG 8 cut(s) 123, 276, 362, 628, 701, 714, 778, 817
BstHHI GCGC 7 cut(s) 123, 628, 714, 746, 778, 817, 945
BstKTI GATC 7 cut(s) 217, 468, 516, 591, 846, 1099, 1175
BstMAI GTCTC 2 cut(s) 61, 891
BstMBI GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
BstMWI GCNNNNNNNGC 5 cut(s) 276, 315, 340, 750, 784
BstNI CCWGG 2 cut(s) 866, 980
BstNSI RCATGY 1 cut(s) 130
BstSCI CCNGG 4 cut(s) 441, 679, 864, 978
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 8 cut(s) 123, 276, 362, 628, 701, 714, 778, 817
BstV2I GAAGAC 3 cut(s) 180, 192, 198
BstX2I RGATCY 2 cut(s) 214, 465
BstXI CCANNNNNNTGG 1 cut(s) 1081
BstYI RGATCY 2 cut(s) 214, 465
BsuRI GGCC 3 cut(s) 334, 528, 761
BtgI CCRYGG 1 cut(s) 523
BtrI CACGTC 2 cut(s) 417, 882
BtsCI GGATG 6 cut(s) 85, 393, 1047, 1075, 1099, 1189
BtsIMutI CAGTG 2 cut(s) 544, 613
Cac8I GCNNGC 2 cut(s) 108, 1146
CfoI GCGC 7 cut(s) 123, 628, 714, 746, 778, 817, 945
Cfr13I GGNCC 3 cut(s) 332, 333, 677
CseI GACGC 3 cut(s) 368, 544, 662
Csp6I GTAC 4 cut(s) 793, 862, 907, 1156
CviAII CATG 6 cut(s) 127, 493, 802, 893, 919, 947
CviQI GTAC 4 cut(s) 793, 862, 907, 1156
DdeI CTNAG 4 cut(s) 139, 573, 606, 888
DpnI GATC 7 cut(s) 216, 467, 515, 590, 845, 1098, 1174
DpnII GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
DraI TTTAAA 1 cut(s) 376
DriI GACNNNNNGTC 1 cut(s) 883
EaeI YGGCCR 2 cut(s) 526, 759
Eam1104I CTCTTC 3 cut(s) 43, 192, 803
Eam1105I GACNNNNNGTC 1 cut(s) 883
EarI CTCTTC 3 cut(s) 43, 192, 803
EciI GGCGGA 1 cut(s) 584
Eco130I CCWWGG 2 cut(s) 1074, 1116
Eco24I GRGCYC 2 cut(s) 336, 371
Eco47I GGWCC 1 cut(s) 677
Eco57I CTGAAG 1 cut(s) 1109
EcoO109I RGGNCCY 1 cut(s) 333
EcoRII CCWGG 2 cut(s) 864, 978
EcoT14I CCWWGG 2 cut(s) 1074, 1116
EcoT38I GRGCYC 2 cut(s) 336, 371
ErhI CCWWGG 2 cut(s) 1074, 1116
Esp3I CGTCTC 1 cut(s) 891
FaeI CATG 6 cut(s) 130, 496, 805, 896, 922, 950
FaiI YATR 8 cut(s) 65, 86, 128, 494, 803, 894, 920, 948
FalI AAGNNNNNCTT 2 cut(s) 94, 126
FaqI GGGAC 1 cut(s) 563
FatI CATG 6 cut(s) 126, 492, 801, 892, 918, 946
FauI CCCGC 2 cut(s) 99, 490
FbaI TGATCA 1 cut(s) 1096
Fnu4HI GCNGC 6 cut(s) 124, 257, 277, 508, 624, 754
FokI GGATG 6 cut(s) 92, 380, 1054, 1082, 1106, 1176
FriOI GRGCYC 2 cut(s) 336, 371
Fsp4HI GCNGC 6 cut(s) 124, 257, 277, 508, 624, 754
GlaI GCGC 7 cut(s) 122, 627, 713, 745, 777, 816, 944
GluI GCNGC 6 cut(s) 124, 257, 277, 508, 624, 754
GsaI CCCAGC 1 cut(s) 333
HaeIII GGCC 3 cut(s) 334, 528, 761
HapII CCGG 4 cut(s) 250, 443, 458, 680
HgaI GACGC 3 cut(s) 368, 544, 662
HhaI GCGC 7 cut(s) 123, 628, 714, 746, 778, 817, 945
Hin1I GRCGYC 2 cut(s) 555, 654
Hin1II CATG 6 cut(s) 130, 496, 805, 896, 922, 950
Hin6I GCGC 7 cut(s) 121, 626, 712, 744, 776, 815, 943
HinP1I GCGC 7 cut(s) 121, 626, 712, 744, 776, 815, 943
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 2 cut(s) 240, 452
HpaII CCGG 4 cut(s) 250, 443, 458, 680
HphI GGTGA 4 cut(s) 715, 817, 1010, 1097
Hpy166II GTNNAC 2 cut(s) 132, 351
Hpy188I TCNGA 5 cut(s) 10, 315, 435, 568, 1140
Hpy188III TCNNGA 2 cut(s) 77, 152
Hpy8I GTNNAC 2 cut(s) 132, 351
HpyAV CCTTC 6 cut(s) 440, 594, 655, 972, 1039, 1084
HpyCH4IV ACGT 2 cut(s) 416, 881
HpyF10VI GCNNNNNNNGC 5 cut(s) 276, 315, 340, 750, 784
HpyF3I CTNAG 4 cut(s) 139, 573, 606, 888
HpySE526I ACGT 2 cut(s) 416, 881
Hsp92I GRCGYC 2 cut(s) 555, 654
Hsp92II CATG 6 cut(s) 130, 496, 805, 896, 922, 950
HspAI GCGC 7 cut(s) 121, 626, 712, 744, 776, 815, 943
KpnI GGTACC 1 cut(s) 865
Ksp22I TGATCA 1 cut(s) 1096
Kzo9I GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
LmnI GCTCC 4 cut(s) 460, 615, 784, 1084
LweI GCATC 5 cut(s) 796, 892, 1060, 1131, 1141
MaeII ACGT 2 cut(s) 416, 881
MaeIII GTNAC 1 cut(s) 518
MalI GATC 7 cut(s) 216, 467, 515, 590, 845, 1098, 1174
MboI GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
MfeI CAATTG 1 cut(s) 1034
MflI RGATCY 2 cut(s) 214, 465
MhlI GDGCHC 2 cut(s) 336, 371
MluCI AATT 4 cut(s) 972, 1020, 1034, 1180
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 1 cut(s) 546
MseI TTAA 2 cut(s) 116, 375
MspI CCGG 4 cut(s) 250, 443, 458, 680
MspR9I CCNGG 4 cut(s) 443, 681, 866, 980
MunI CAATTG 1 cut(s) 1034
Mva1269I GAATGC 1 cut(s) 94
MvaI CCWGG 2 cut(s) 866, 980
MvnI CGCG 8 cut(s) 123, 276, 362, 628, 701, 714, 778, 817
MwoI GCNNNNNNNGC 5 cut(s) 276, 315, 340, 750, 784
NciI CCSGG 2 cut(s) 443, 681
NdeII GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
NlaIII CATG 6 cut(s) 130, 496, 805, 896, 922, 950
NlaIV GGNNCC 6 cut(s) 216, 334, 335, 678, 738, 863
NmuCI GTSAC 1 cut(s) 518
NspI RCATGY 1 cut(s) 130
PcsI WCGNNNNNNNCGW 6 cut(s) 49, 165, 168, 174, 295, 648
PctI GAATGC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 452
PflFI GACNNNGTC 2 cut(s) 72, 554
PfoI TCCNGGA 1 cut(s) 679
PkrI GCNGC 6 cut(s) 125, 258, 278, 509, 625, 755
PleI GAGTC 1 cut(s) 234
PpsI GAGTC 1 cut(s) 234
Psp6I CCWGG 2 cut(s) 864, 978
PspFI CCCAGC 1 cut(s) 329
PspGI CCWGG 2 cut(s) 864, 978
PspN4I GGNNCC 6 cut(s) 216, 334, 335, 678, 738, 863
PspOMI GGGCCC 1 cut(s) 332
PspPI GGNCC 3 cut(s) 332, 333, 677
PsuI RGATCY 2 cut(s) 214, 465
PsyI GACNNNGTC 2 cut(s) 72, 554
RsaI GTAC 4 cut(s) 794, 863, 908, 1157
RsaNI GTAC 4 cut(s) 793, 862, 907, 1156
SaqAI TTAA 2 cut(s) 116, 375
SatI GCNGC 6 cut(s) 124, 257, 277, 508, 624, 754
Sau3AI GATC 7 cut(s) 214, 465, 513, 588, 843, 1096, 1172
Sau96I GGNCC 3 cut(s) 332, 333, 677
SchI GAGTC 1 cut(s) 234
ScrFI CCNGG 4 cut(s) 443, 681, 866, 980
SduI GDGCHC 2 cut(s) 336, 371
SfaNI GCATC 5 cut(s) 796, 892, 1060, 1131, 1141
SinI GGWCC 1 cut(s) 677
SmlI CTYRAG 1 cut(s) 1160
SmoI CTYRAG 1 cut(s) 1160
Sse9I AATT 4 cut(s) 972, 1020, 1034, 1180
StyD4I CCNGG 4 cut(s) 441, 679, 864, 978
StyI CCWWGG 2 cut(s) 1074, 1116
TaiI ACGT 2 cut(s) 419, 884
TaqI TCGA 4 cut(s) 15, 298, 587, 651
TasI AATT 4 cut(s) 972, 1020, 1034, 1180
TauI GCSGC 6 cut(s) 126, 259, 279, 510, 626, 756
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 2 cut(s) 116, 375
Tru9I TTAA 2 cut(s) 116, 375
TscAI CASTG 2 cut(s) 551, 620
TseFI GTSAC 1 cut(s) 518
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 5 cut(s) 17, 101, 198, 460, 1058
TspGWI ACGGA 2 cut(s) 506, 552
TspRI CASTG 2 cut(s) 551, 620
Tth111I GACNNNGTC 2 cut(s) 72, 554
VpaK11BI GGWCC 1 cut(s) 677
XceI RCATGY 1 cut(s) 130
XcmI CCANNNNNNNNNTGG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.