Rmu_sc0002972.1_g000002

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002972.1
OG0002553
Physical Location & Seq
Reverse (-)
1871 .. 2896
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1026 bp
atgataggcagcggcacaaaactactcggcgaggcagcaagaagtatggttaaagagattaaggagtatgtaacaggaaaggaagtcgggactatacgtgtttatggaatggctggtttcggcaagacagcagttgtgtcggaagttaatgacctcatagtgaatgactacagaagttgtgccccagaaggtacaaataacggcagtagatacactgaaaaccattttgacaaaattatatgggttactgtagagcatcgaggatcaactcaatcatctattgacaacttgcagaataaaattgcagagaaattacatattgatttgtgcataaagccactgggagtagagcgcgcagatacatatgaaagtgcattgaaggagttgagatttttgatcattctggatgatatgcggaagaagctgtctcttgagctaattgggattctaccaacgagggaaaatggctgcaaggttgtaatagtatctagatcacttccagtgttcagtgacatcaaaattgataagaaggttgagatcaaacctctttcagatacagcggcacgagaattgtttgagagggaagctggcattagattctcagacttgagggatgatactcaaactatggcagagaaaatggtagatgactgtgatagtttgcccttggctattggctttctggctcggatcatgaaagaattaaagagtgatgctgctgagagtgtcgatgctgcacggaatgcagcccttttgaagttacagagttctcacttggaaagcatgaatgaaaaggcattcgctcgtctaaagtacagctatgaaatattgaagaaagatgctccgcaatgtctcctgtattgtgccttgtacccatgtggtcattacattggtaaaaaagagctagtggagtattggttttgggatggtttattggatagaaacaagagagatggacggatagaagatatggtacaagcaagagacatactggatgagctcgtacggccttctggaaacagttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_4 LRR_8 NB-ARC

Protein Analysis

341

Amino Acids

38.41

Weight (kDa)

5.9

Isoelectric Point (pI)

28.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 69 - 196 1.7e-14 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 354
AciI CCGC 4 cut(s) 12, 415, 560, 845
AclWI GGATC 2 cut(s) 271, 698
AfaI GTAC 5 cut(s) 193, 815, 872, 975, 1005
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 3 cut(s) 379, 757, 832
AluBI AGCT 6 cut(s) 424, 436, 587, 819, 904, 1000
AluI AGCT 6 cut(s) 424, 436, 587, 819, 904, 1000
Alw21I GWGCWC 1 cut(s) 1002
Alw26I GTCTC 3 cut(s) 432, 857, 978
AlwI GGATC 2 cut(s) 271, 698
AoxI GGCC 1 cut(s) 1007
ApeKI GCWGC 6 cut(s) 9, 35, 468, 716, 734, 746
AspLEI GCGC 2 cut(s) 354, 356
BaeGI GKGCMC 1 cut(s) 184
BanII GRGCYC 1 cut(s) 1002
BarI GAAGNNNNNNTAC 2 cut(s) 957, 989
BauI CACGAG 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 1002
BbvI GCAGC 6 cut(s) 21, 47, 455, 703, 721, 758
BccI CCATC 2 cut(s) 920, 947
BceAI ACGGC 2 cut(s) 217, 1022
BcgI CGANNNNNNTGC 2 cut(s) 120, 154
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 3 cut(s) 432, 857, 978
BfaI CTAG 2 cut(s) 489, 905
BfmI CTRYAG 2 cut(s) 169, 249
BisI GCNGC 8 cut(s) 10, 13, 36, 469, 561, 717, 735, 747
BlsI GCNGC 8 cut(s) 11, 14, 37, 470, 562, 718, 736, 748
BmrI ACTGGG 1 cut(s) 350
BmsI GCATC 4 cut(s) 265, 703, 721, 829
BmuI ACTGGG 1 cut(s) 350
BpuEI CTTGAG 2 cut(s) 452, 628
BsaAI YACGTR 1 cut(s) 98
BsaJI CCNNGG 1 cut(s) 666
BsaXI ACNNNNNCTCC 2 cut(s) 902, 932
Bse1I ACTGG 3 cut(s) 345, 500, 996
Bse3DI GCAATG 1 cut(s) 854
BseDI CCNNGG 1 cut(s) 666
BseGI GGATG 4 cut(s) 412, 619, 931, 1000
BseMI GCAATG 1 cut(s) 854
BseMII CTCAG 2 cut(s) 615, 711
BseNI ACTGG 3 cut(s) 345, 500, 996
BsePI GCGCGC 1 cut(s) 352
BseSI GKGCMC 1 cut(s) 184
BseXI GCAGC 6 cut(s) 21, 47, 455, 703, 721, 758
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 1 cut(s) 354
BshFI GGCC 1 cut(s) 1009
BsiHKAI GWGCWC 1 cut(s) 1002
BsiWI CGTACG 1 cut(s) 1003
BslFI GGGAC 1 cut(s) 103
BsmAI GTCTC 3 cut(s) 432, 857, 978
BsmFI GGGAC 1 cut(s) 103
BsmI GAATGC 2 cut(s) 748, 797
BsnI GGCC 1 cut(s) 1009
Bsp1286I GDGCHC 2 cut(s) 184, 1002
Bsp143I GATC 5 cut(s) 263, 396, 491, 537, 690
BspACI CCGC 4 cut(s) 12, 415, 560, 845
BspANI GGCC 1 cut(s) 1009
BspCNI CTCAG 2 cut(s) 614, 712
BspFNI CGCG 1 cut(s) 354
BspHI TCATGA 1 cut(s) 693
BspPI GGATC 2 cut(s) 271, 698
BsrDI GCAATG 1 cut(s) 854
BsrI ACTGG 3 cut(s) 345, 500, 996
BssECI CCNNGG 1 cut(s) 666
BssHII GCGCGC 1 cut(s) 352
BssMI GATC 5 cut(s) 263, 396, 491, 537, 690
BssSI CACGAG 1 cut(s) 564
BssT1I CCWWGG 1 cut(s) 666
Bst2BI CACGAG 1 cut(s) 564
Bst4CI ACNGT 3 cut(s) 250, 653, 1022
BstAPI GCANNNNNTGC 1 cut(s) 743
BstBAI YACGTR 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 354, 589
BstDEI CTNAG 2 cut(s) 601, 720
BstF5I GGATG 4 cut(s) 412, 619, 931, 1000
BstFNI CGCG 1 cut(s) 354
BstHHI GCGC 2 cut(s) 354, 356
BstKTI GATC 5 cut(s) 266, 399, 494, 540, 693
BstMAI GTCTC 3 cut(s) 432, 857, 978
BstMBI GATC 5 cut(s) 263, 396, 491, 537, 690
BstMWI GCNNNNNNNGC 3 cut(s) 421, 743, 1006
BstSFI CTRYAG 2 cut(s) 169, 249
BstSLI GKGCMC 1 cut(s) 184
BstUI CGCG 1 cut(s) 354
BstV1I GCAGC 6 cut(s) 21, 47, 455, 703, 721, 758
BsuRI GGCC 1 cut(s) 1009
BtsCI GGATG 4 cut(s) 412, 619, 931, 1000
BtsIMutI CAGTG 4 cut(s) 213, 338, 507, 514
Cac8I GCNNGC 2 cut(s) 354, 589
CciI TCATGA 1 cut(s) 693
CfoI GCGC 2 cut(s) 354, 356
Csp6I GTAC 5 cut(s) 192, 814, 871, 974, 1004
CviAII CATG 3 cut(s) 694, 784, 876
CviQI GTAC 5 cut(s) 192, 814, 871, 974, 1004
DdeI CTNAG 2 cut(s) 601, 720
DpnI GATC 5 cut(s) 265, 398, 493, 539, 692
DpnII GATC 5 cut(s) 263, 396, 491, 537, 690
Ecl136II GAGCTC 1 cut(s) 1000
Eco130I CCWWGG 1 cut(s) 666
Eco24I GRGCYC 1 cut(s) 1002
Eco53kI GAGCTC 1 cut(s) 1000
EcoICRI GAGCTC 1 cut(s) 1000
EcoT14I CCWWGG 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 1002
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 3 cut(s) 697, 787, 879
FaqI GGGAC 1 cut(s) 103
FatI CATG 3 cut(s) 693, 783, 875
FauNDI CATATG 1 cut(s) 364
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 8 cut(s) 10, 13, 36, 469, 561, 717, 735, 747
FokI GGATG 4 cut(s) 419, 626, 938, 1007
FriOI GRGCYC 1 cut(s) 1002
Fsp4HI GCNGC 8 cut(s) 10, 13, 36, 469, 561, 717, 735, 747
FspBI CTAG 2 cut(s) 489, 905
GlaI GCGC 2 cut(s) 353, 355
GluI GCNGC 8 cut(s) 10, 13, 36, 469, 561, 717, 735, 747
HaeIII GGCC 1 cut(s) 1009
HhaI GCGC 2 cut(s) 354, 356
Hin1II CATG 3 cut(s) 697, 787, 879
Hin6I GCGC 2 cut(s) 352, 354
HinP1I GCGC 2 cut(s) 352, 354
HinfI GANTC 2 cut(s) 445, 597
Hpy188I TCNGA 4 cut(s) 142, 553, 604, 690
Hpy188III TCNNGA 6 cut(s) 88, 404, 431, 489, 694, 1014
HpyAV CCTTC 4 cut(s) 182, 373, 523, 1020
HpyCH4III ACNGT 3 cut(s) 250, 653, 1022
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 7 cut(s) 292, 305, 330, 374, 471, 737, 746
HpyF10VI GCNNNNNNNGC 3 cut(s) 421, 743, 1006
HpyF3I CTNAG 2 cut(s) 601, 720
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 3 cut(s) 697, 787, 879
HspAI GCGC 2 cut(s) 352, 354
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 5 cut(s) 263, 396, 491, 537, 690
LmnI GCTCC 1 cut(s) 847
Lsp1109I GCAGC 6 cut(s) 21, 47, 455, 703, 721, 758
LweI GCATC 4 cut(s) 265, 703, 721, 829
MaeI CTAG 2 cut(s) 489, 905
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 4 cut(s) 70, 244, 509, 759
MalI GATC 5 cut(s) 265, 398, 493, 539, 692
MboI GATC 5 cut(s) 263, 396, 491, 537, 690
MboII GAAGA 3 cut(s) 430, 844, 977
MhlI GDGCHC 2 cut(s) 184, 1002
MluCI AATT 7 cut(s) 234, 300, 311, 438, 519, 569, 701
MmeI TCCRAC 1 cut(s) 120
MnlI CCTC 7 cut(s) 25, 164, 254, 450, 555, 573, 603
MseI TTAA 4 cut(s) 51, 60, 147, 704
MspA1I CMGCKG 2 cut(s) 12, 560
Mva1269I GAATGC 2 cut(s) 748, 797
MvnI CGCG 1 cut(s) 354
MwoI GCNNNNNNNGC 3 cut(s) 421, 743, 1006
NdeI CATATG 1 cut(s) 364
NdeII GATC 5 cut(s) 263, 396, 491, 537, 690
NlaIII CATG 3 cut(s) 697, 787, 879
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 509
PagI TCATGA 1 cut(s) 693
PauI GCGCGC 1 cut(s) 352
PctI GAATGC 2 cut(s) 748, 797
PfeI GAWTC 2 cut(s) 445, 597
Pfl23II CGTACG 1 cut(s) 1003
PkrI GCNGC 8 cut(s) 11, 14, 37, 470, 562, 718, 736, 748
Ppu21I YACGTR 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 1002
PspLI CGTACG 1 cut(s) 1003
PteI GCGCGC 1 cut(s) 352
RsaI GTAC 5 cut(s) 193, 815, 872, 975, 1005
RsaNI GTAC 5 cut(s) 192, 814, 871, 974, 1004
SacI GAGCTC 1 cut(s) 1002
SaqAI TTAA 4 cut(s) 51, 60, 147, 704
SatI GCNGC 8 cut(s) 10, 13, 36, 469, 561, 717, 735, 747
Sau3AI GATC 5 cut(s) 263, 396, 491, 537, 690
SduI GDGCHC 2 cut(s) 184, 1002
SfaNI GCATC 4 cut(s) 265, 703, 721, 829
SfcI CTRYAG 2 cut(s) 169, 249
SmlI CTYRAG 2 cut(s) 431, 607
SmoI CTYRAG 2 cut(s) 431, 607
Sse9I AATT 7 cut(s) 234, 300, 311, 438, 519, 569, 701
SsiI CCGC 4 cut(s) 12, 415, 560, 845
SspI AATATT 1 cut(s) 828
SspMI CTAG 2 cut(s) 489, 905
SstI GAGCTC 1 cut(s) 1002
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 3 cut(s) 250, 653, 1022
TaiI ACGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 259, 729
TasI AATT 7 cut(s) 234, 300, 311, 438, 519, 569, 701
TatI WGTACW 1 cut(s) 813
TauI GCSGC 2 cut(s) 15, 563
TfiI GAWTC 2 cut(s) 445, 597
Tru1I TTAA 4 cut(s) 51, 60, 147, 704
Tru9I TTAA 4 cut(s) 51, 60, 147, 704
TscAI CASTG 4 cut(s) 220, 345, 507, 514
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 6 cut(s) 9, 35, 468, 716, 734, 746
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 5 cut(s) 381, 710, 800, 804, 837
TspGWI ACGGA 2 cut(s) 754, 973
TspRI CASTG 4 cut(s) 220, 345, 507, 514
XbaI TCTAGA 1 cut(s) 488
XspI CTAG 2 cut(s) 489, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.