Rmu_sc0003435.1_g000020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003435.1
OG0003279
Physical Location & Seq
Forward (+)
59227 .. 61103
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003435.1_g000020.1.cds

Sequence Viewer

Length: 845 bp
atgcgccaccagtacctgggtgtcaagcagaagatccgggtatccaaggacgagttcaactggaaggatggtgaaaatcactgggagaatttcttgaagtacaaggaagtgtttggtgacgtggagcttgatggtaagggcaagaggtcgtgtgaaaatgacaactttgatgtttttggggattgtggggacttggggtttggtattgattgtgaggatttagaggaaggagaagatgatgaagaagaagaagaacatttggaagaagaagaagaaggtggtactggtggtgatgacgataatcgtggcgaggaattgggtaaaactgaaattggtgagtttgggggtgaaagggaaattggggacatgggaatggctcaaaaaaggaaattgagtaaggttggattgcataggaggttggggttgatgagtgctcaggtgttggacttgcagaatatgtttgtgaagagggaagaggggaggagagagagggagtgtaggagagagaaaggtgaggctgagagagaggaaaggaggaaagaagttgagtttaggagagagaataggaggaatgagcaggaggacttgttagaagatagagaattggagttgcaagagagagaggccatgtggacaagaagggagtttgagaaacggttgaggttacagggggagtttgatgaggagcgtcagaggaggatgaggatggaggagaggagggaggaggaggagttggaggggcgggagaggatggtaggtttgcaaattcagcatgagaagcaaatgatgcaaatccatgctgaggcatgccagaaccaaatgcagattcttggggtcatggctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

281

Amino Acids

33.63

Weight (kDa)

4.78

Isoelectric Point (pI)

68.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 16
AciI CCGC 1 cut(s) 742
AclWI GGATC 1 cut(s) 28
AcsI RAATTY 2 cut(s) 88, 765
AfaI GTAC 3 cut(s) 14, 101, 283
AfiI CCNNNNNNNGG 2 cut(s) 16, 802
AgsI TTSAA 2 cut(s) 58, 97
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 1 cut(s) 15
AjuI GAANNNNNNNTTGG 6 cut(s) 38, 70, 342, 374, 810, 842
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw21I GWGCWC 1 cut(s) 436
AlwI GGATC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 16
AoxI GGCC 1 cut(s) 624
ApoI RAATTY 2 cut(s) 88, 765
ArsI GACNNNNNNTTYG 2 cut(s) 182, 214
AspLEI GCGC 1 cut(s) 6
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 6 cut(s) 83, 128, 302, 347, 359, 524
Bbv12I GWGCWC 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 801
BccI CCATC 4 cut(s) 62, 125, 700, 745
BciT130I CCWGG 1 cut(s) 17
BciVI GTATCC 1 cut(s) 52
BcnI CCSGG 1 cut(s) 38
BfaI CTAG 1 cut(s) 843
BfuI GTATCC 1 cut(s) 52
Bme1390I CCNGG 2 cut(s) 17, 38
BmgBI CACGTC 1 cut(s) 121
BmrFI CCNGG 2 cut(s) 17, 38
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 1 cut(s) 777
BmuI ACTGGG 1 cut(s) 91
Bpu10I CCTNAGC 2 cut(s) 435, 801
BpuMI CCSGG 1 cut(s) 38
BsaBI GATNNNNATC 1 cut(s) 791
BsaJI CCNNGG 2 cut(s) 16, 45
BsaXI ACNNNNNCTCC 4 cut(s) 572, 602, 716, 746
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 802
Bse1I ACTGG 4 cut(s) 10, 65, 86, 289
Bse8I GATNNNNATC 1 cut(s) 791
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 2 cut(s) 16, 45
BseGI GGATG 4 cut(s) 73, 705, 711, 756
BseJI GATNNNNATC 1 cut(s) 791
BseLI CCNNNNNNNGG 2 cut(s) 16, 802
BseMII CTCAG 3 cut(s) 449, 510, 792
BseNI ACTGG 4 cut(s) 10, 65, 86, 289
BseRI GAGGAG 8 cut(s) 496, 698, 709, 725, 730, 737, 740, 743
BshFI GGCC 1 cut(s) 626
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 1 cut(s) 37
BslFI GGGAC 2 cut(s) 203, 377
BslI CCNNNNNNNGG 2 cut(s) 16, 802
BsmFI GGGAC 2 cut(s) 203, 377
BsnI GGCC 1 cut(s) 626
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 1 cut(s) 33
BspACI CCGC 1 cut(s) 742
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 3 cut(s) 448, 511, 793
BspPI GGATC 1 cut(s) 28
BsrI ACTGG 4 cut(s) 10, 65, 86, 289
BssECI CCNNGG 2 cut(s) 16, 45
BssMI GATC 1 cut(s) 33
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 1 cut(s) 657
Bst6I CTCTTC 2 cut(s) 461, 468
BstAPI GCANNNNNTGC 1 cut(s) 787
BstC8I GCNNGC 1 cut(s) 808
BstDEI CTNAG 3 cut(s) 435, 519, 801
BstF5I GGATG 4 cut(s) 73, 705, 711, 756
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 1 cut(s) 36
BstMBI GATC 1 cut(s) 33
BstMWI GCNNNNNNNGC 3 cut(s) 769, 778, 787
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 1 cut(s) 810
BstSCI CCNGG 2 cut(s) 15, 36
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BsuI GTATCC 1 cut(s) 52
BsuRI GGCC 1 cut(s) 626
BtrI CACGTC 1 cut(s) 121
BtsCI GGATG 4 cut(s) 73, 705, 711, 756
BtsIMutI CAGTG 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 808
CaiI CAGNNNCTG 1 cut(s) 16
CfoI GCGC 1 cut(s) 6
CseI GACGC 1 cut(s) 677
Csp6I GTAC 3 cut(s) 13, 100, 282
CviAII CATG 6 cut(s) 367, 628, 773, 797, 807, 838
CviJI RGCY 5 cut(s) 127, 377, 518, 626, 842
CviKI_1 RGCY 5 cut(s) 127, 377, 518, 626, 842
CviQI GTAC 3 cut(s) 13, 100, 282
DdeI CTNAG 3 cut(s) 435, 519, 801
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
Eam1104I CTCTTC 2 cut(s) 461, 468
EarI CTCTTC 2 cut(s) 461, 468
Eco130I CCWWGG 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 6 cut(s) 370, 631, 776, 800, 810, 841
FaiI YATR 8 cut(s) 368, 411, 458, 629, 774, 798, 808, 839
FaqI GGGAC 2 cut(s) 203, 377
FatI CATG 6 cut(s) 366, 627, 772, 796, 806, 837
FauI CCCGC 1 cut(s) 735
FokI GGATG 4 cut(s) 80, 712, 718, 763
FspBI CTAG 1 cut(s) 843
GlaI GCGC 1 cut(s) 5
HaeIII GGCC 1 cut(s) 626
HapII CCGG 1 cut(s) 37
HgaI GACGC 1 cut(s) 677
HhaI GCGC 1 cut(s) 6
Hin1II CATG 6 cut(s) 370, 631, 776, 800, 810, 841
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HinfI GANTC 1 cut(s) 826
HpaII CCGG 1 cut(s) 37
HphI GGTGA 6 cut(s) 83, 128, 302, 347, 359, 524
Hpy166II GTNNAC 1 cut(s) 633
Hpy188I TCNGA 1 cut(s) 693
Hpy188III TCNNGA 1 cut(s) 94
Hpy8I GTNNAC 1 cut(s) 633
HpyAV CCTTC 4 cut(s) 58, 221, 269, 633
HpyCH4III ACNGT 1 cut(s) 657
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 6 cut(s) 409, 451, 613, 763, 790, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 769, 778, 787
HpyF3I CTNAG 3 cut(s) 435, 519, 801
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 6 cut(s) 370, 631, 776, 800, 810, 841
HspAI GCGC 1 cut(s) 4
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 124, 685
LweI GCATC 1 cut(s) 777
MaeI CTAG 1 cut(s) 843
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 2 cut(s) 116, 663
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 1 cut(s) 436
MluCI AATT 7 cut(s) 88, 314, 330, 357, 389, 602, 765
MmeI TCCRAC 3 cut(s) 382, 423, 714
MslI CAYNNNNRTG 1 cut(s) 371
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 2 cut(s) 17, 38
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 769, 778, 787
NciI CCSGG 1 cut(s) 38
NdeII GATC 1 cut(s) 33
NlaIII CATG 6 cut(s) 370, 631, 776, 800, 810, 841
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 810
PaeI GCATGC 1 cut(s) 810
PfeI GAWTC 1 cut(s) 826
PflMI CCANNNNNTGG 1 cut(s) 16
Psp6I CCWGG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 15
PstNI CAGNNNCTG 1 cut(s) 16
PsuI RGATCY 1 cut(s) 33
RsaI GTAC 3 cut(s) 14, 101, 283
RsaNI GTAC 3 cut(s) 13, 100, 282
RseI CAYNNNNRTG 1 cut(s) 371
Sau3AI GATC 1 cut(s) 33
ScrFI CCNGG 2 cut(s) 17, 38
SduI GDGCHC 1 cut(s) 436
SfaNI GCATC 1 cut(s) 777
SmiMI CAYNNNNRTG 1 cut(s) 371
SphI GCATGC 1 cut(s) 810
Sse9I AATT 7 cut(s) 88, 314, 330, 357, 389, 602, 765
SsiI CCGC 1 cut(s) 742
SspMI CTAG 1 cut(s) 843
StyD4I CCNGG 2 cut(s) 15, 36
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 657
TaiI ACGT 1 cut(s) 123
TasI AATT 7 cut(s) 88, 314, 330, 357, 389, 602, 765
TatI WGTACW 1 cut(s) 99
TfiI GAWTC 1 cut(s) 826
TscAI CASTG 1 cut(s) 86
TseFI GTSAC 1 cut(s) 116
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 1 cut(s) 255
TspRI CASTG 1 cut(s) 86
Van91I CCANNNNNTGG 1 cut(s) 16
XapI RAATTY 2 cut(s) 88, 765
XceI RCATGY 1 cut(s) 810
XspI CTAG 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.