Rorug07G0202800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0003279
Physical Location & Seq
Forward (+)
17752305 .. 17753754
1450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0202800.1

Sequence Viewer

Length: 750 bp
ATGGTGAAGATTGGTTTTGGTAGCTTTGGTGACTCTTTCAGTGTTGGCTCCCTCAAGTCCTACCTAGCCGAGTTCATCGCCACCCTTCTTTTCGTGTTTGCTGGCGTTGGCTCTGCCCTCGCTTATGATAAGCTGACTACAGATGCAGCTTTAGACCCTGCCGGCCTAGTCGCTGTGGCCGTGGCCCATGCATTTGCTCTGTTCGTGGGGGTCTCCGTTGCTGCAAACATCTCCGGTGGCCATTTGAACCCAGCTGTCACTTTCGGATTGGCTGTTGGAGGCAACATTACCATCCTAACCGGCATTTTCTACTGGATTGCTCAACTCTTAGGCTCCATCGTCGCTTGCTTTCTCCTCAAGTTTGTCACCAGCGAGAGTGTGCCAACACATGGACTTGCCTCTGGTGTAGGAGCTATTGAAGGTGTTGTGTTTGAAATCATCATAACCTTTGGTCTGGTCTACACTGTCTATGCCACAGCCGCAGACCCCAAGAAGGGTTCACTTGGGATCATAGCACCCATTGCAATAGGGTTCATTGTTGGTGCCAACATCCTAGCGGCCGGACCATTCAGCGGTGGATCTATGAACCCAGCTCGCTCATTTGGCCCGGCCGTTGTCAGCGGAAACTTCGCCGATAACTGGATCTATTGGGTTGGTCCATTAGTTGGTGGAGGTTTGGCTGGCCTGGTCTATGGTGGCATTTTCATTGGATCCTACTCCCCAGTTCCAGTTTCTGAAGATTATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

25.13

Weight (kDa)

4.9

Isoelectric Point (pI)

29.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 14 - 231 1.5e-80 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 542
AccB7I CCANNNNNTGG 2 cut(s) 389, 665
AccI GTMKAC 1 cut(s) 459
AciI CCGC 4 cut(s) 480, 557, 573, 621
AclWI GGATC 5 cut(s) 515, 586, 650, 705, 718
AcoI YGGCCR 4 cut(s) 177, 238, 558, 609
AfiI CCNNNNNNNGG 6 cut(s) 389, 493, 494, 572, 639, 665
AgsI TTSAA 3 cut(s) 247, 419, 434
AjnI CCWGG 1 cut(s) 684
AluBI AGCT 6 cut(s) 24, 133, 149, 254, 413, 593
AluI AGCT 6 cut(s) 24, 133, 149, 254, 413, 593
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 5 cut(s) 515, 586, 650, 705, 718
AlwNI CAGNNNCTG 1 cut(s) 734
AoxI GGCC 8 cut(s) 163, 177, 183, 238, 558, 604, 609, 682
ApeKI GCWGC 2 cut(s) 146, 221
AspS9I GGNCC 4 cut(s) 184, 563, 605, 656
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 3 cut(s) 16, 41, 358
AvaII GGWCC 2 cut(s) 563, 656
BalI TGGCCA 1 cut(s) 240
BamHI GGATCC 1 cut(s) 710
BanI GGYRCC 1 cut(s) 542
BbvI GCAGC 2 cut(s) 158, 208
BccI CCATC 2 cut(s) 299, 344
BceAI ACGGC 2 cut(s) 164, 596
BciT130I CCWGG 1 cut(s) 686
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 3 cut(s) 65, 167, 554
BfmI CTRYAG 1 cut(s) 138
BisI GCNGC 4 cut(s) 147, 222, 480, 558
BlsI GCNGC 4 cut(s) 148, 223, 481, 559
Bme1390I CCNGG 2 cut(s) 608, 686
Bme18I GGWCC 2 cut(s) 563, 656
BmgT120I GGNCC 4 cut(s) 184, 563, 605, 656
BmiI GGNNCC 4 cut(s) 49, 334, 544, 712
BmrFI CCNGG 2 cut(s) 608, 686
BmrI ACTGGG 1 cut(s) 716
BmsI GCATC 1 cut(s) 133
BmuI ACTGGG 1 cut(s) 716
BpuEI CTTGAG 2 cut(s) 38, 341
BpuMI CCSGG 1 cut(s) 608
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 180
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 6 cut(s) 389, 493, 494, 572, 639, 665
Bse118I RCCGGY 2 cut(s) 161, 299
Bse1I ACTGG 4 cut(s) 317, 644, 722, 728
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 1 cut(s) 686
BseDI CCNNGG 1 cut(s) 180
BseGI GGATG 2 cut(s) 291, 549
BseLI CCNNNNNNNGG 6 cut(s) 389, 493, 494, 572, 639, 665
BseMI GCAATG 1 cut(s) 519
BseNI ACTGG 4 cut(s) 317, 644, 722, 728
BseRI GAGGAG 1 cut(s) 344
BseX3I CGGCCG 2 cut(s) 558, 609
BseXI GCAGC 2 cut(s) 158, 208
BseYI CCCAGC 2 cut(s) 250, 589
Bsh1285I CGRYCG 2 cut(s) 561, 612
BshFI GGCC 8 cut(s) 165, 179, 185, 240, 560, 606, 611, 684
BshNI GGYRCC 1 cut(s) 542
BsiEI CGRYCG 2 cut(s) 561, 612
BsiSI CCGG 5 cut(s) 162, 234, 300, 561, 608
BslI CCNNNNNNNGG 6 cut(s) 389, 493, 494, 572, 639, 665
BsmAI GTCTC 1 cut(s) 217
BsnI GGCC 8 cut(s) 165, 179, 185, 240, 560, 606, 611, 684
Bso31I GGTCTC 1 cut(s) 217
Bsp143I GATC 4 cut(s) 507, 578, 642, 710
BspACI CCGC 4 cut(s) 480, 557, 573, 621
BspANI GGCC 8 cut(s) 165, 179, 185, 240, 560, 606, 611, 684
BspLI GGNNCC 4 cut(s) 49, 334, 544, 712
BspPI GGATC 5 cut(s) 515, 586, 650, 705, 718
BspT107I GGYRCC 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 161, 299
BsrI ACTGG 4 cut(s) 317, 644, 722, 728
BssAI RCCGGY 2 cut(s) 161, 299
BssECI CCNNGG 1 cut(s) 180
BssMI GATC 4 cut(s) 507, 578, 642, 710
Bst2UI CCWGG 1 cut(s) 686
Bst4CI ACNGT 1 cut(s) 466
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 5 cut(s) 103, 163, 346, 595, 682
BstDEI CTNAG 1 cut(s) 328
BstDSI CCRYGG 1 cut(s) 180
BstF5I GGATG 2 cut(s) 291, 549
BstKTI GATC 4 cut(s) 510, 581, 645, 713
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 4 cut(s) 507, 578, 642, 710
BstMCI CGRYCG 2 cut(s) 561, 612
BstMWI GCNNNNNNNGC 3 cut(s) 479, 521, 603
BstNI CCWGG 1 cut(s) 686
BstSCI CCNGG 2 cut(s) 606, 684
BstSFI CTRYAG 1 cut(s) 138
BstV1I GCAGC 2 cut(s) 158, 208
BstX2I RGATCY 3 cut(s) 578, 642, 710
BstYI RGATCY 3 cut(s) 578, 642, 710
BstZI CGGCCG 2 cut(s) 558, 609
BsuRI GGCC 8 cut(s) 165, 179, 185, 240, 560, 606, 611, 684
BtgI CCRYGG 1 cut(s) 180
BtgZI GCGATG 1 cut(s) 61
BtsCI GGATG 2 cut(s) 291, 549
BtsIMutI CAGTG 2 cut(s) 46, 462
Cac8I GCNNGC 5 cut(s) 103, 163, 346, 595, 682
CaiI CAGNNNCTG 1 cut(s) 734
Cfr10I RCCGGY 2 cut(s) 161, 299
Cfr13I GGNCC 4 cut(s) 184, 563, 605, 656
CviAII CATG 2 cut(s) 188, 389
DdeI CTNAG 1 cut(s) 328
DpnI GATC 4 cut(s) 509, 580, 644, 712
DpnII GATC 4 cut(s) 507, 578, 642, 710
EaeI YGGCCR 4 cut(s) 177, 238, 558, 609
EagI CGGCCG 2 cut(s) 558, 609
EclXI CGGCCG 2 cut(s) 558, 609
Eco31I GGTCTC 1 cut(s) 217
Eco47I GGWCC 2 cut(s) 563, 656
Eco52I CGGCCG 2 cut(s) 558, 609
EcoRII CCWGG 1 cut(s) 684
EcoT22I ATGCAT 1 cut(s) 193
FaeI CATG 2 cut(s) 191, 392
FaiI YATR 9 cut(s) 126, 189, 390, 443, 471, 512, 584, 693, 744
FatI CATG 2 cut(s) 187, 388
FblI GTMKAC 1 cut(s) 459
Fnu4HI GCNGC 4 cut(s) 147, 222, 480, 558
FokI GGATG 2 cut(s) 278, 536
Fsp4HI GCNGC 4 cut(s) 147, 222, 480, 558
FspBI CTAG 3 cut(s) 65, 167, 554
GluI GCNGC 4 cut(s) 147, 222, 480, 558
GsaI CCCAGC 2 cut(s) 254, 593
HaeIII GGCC 8 cut(s) 165, 179, 185, 240, 560, 606, 611, 684
HapII CCGG 5 cut(s) 162, 234, 300, 561, 608
Hin1II CATG 2 cut(s) 191, 392
HinfI GANTC 1 cut(s) 32
HpaII CCGG 5 cut(s) 162, 234, 300, 561, 608
HphI GGTGA 3 cut(s) 16, 41, 358
Hpy166II GTNNAC 2 cut(s) 460, 500
Hpy188I TCNGA 2 cut(s) 266, 736
Hpy8I GTNNAC 2 cut(s) 460, 500
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 3 cut(s) 95, 413, 487
HpyCH4III ACNGT 1 cut(s) 466
HpyCH4V TGCA 4 cut(s) 146, 191, 224, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 479, 521, 603
HpyF3I CTNAG 1 cut(s) 328
Hsp92II CATG 2 cut(s) 191, 392
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 4 cut(s) 507, 578, 642, 710
LmnI GCTCC 3 cut(s) 53, 338, 410
Lsp1109I GCAGC 2 cut(s) 158, 208
LweI GCATC 1 cut(s) 133
MaeI CTAG 3 cut(s) 65, 167, 554
MaeIII GTNAC 3 cut(s) 29, 256, 364
MalI GATC 4 cut(s) 509, 580, 644, 712
MboI GATC 4 cut(s) 507, 578, 642, 710
MboII GAAGA 2 cut(s) 19, 749
MflI RGATCY 3 cut(s) 578, 642, 710
MlsI TGGCCA 1 cut(s) 240
MluNI TGGCCA 1 cut(s) 240
MlyI GAGTC 1 cut(s) 26
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 6 cut(s) 62, 128, 272, 365, 409, 665
Mox20I TGGCCA 1 cut(s) 240
Mph1103I ATGCAT 1 cut(s) 193
MroNI GCCGGC 1 cut(s) 161
MscI TGGCCA 1 cut(s) 240
Msp20I TGGCCA 1 cut(s) 240
MspA1I CMGCKG 3 cut(s) 254, 573, 621
MspI CCGG 5 cut(s) 162, 234, 300, 561, 608
MspR9I CCNGG 2 cut(s) 608, 686
MvaI CCWGG 1 cut(s) 686
MwoI GCNNNNNNNGC 3 cut(s) 479, 521, 603
NaeI GCCGGC 1 cut(s) 163
NciI CCSGG 1 cut(s) 608
NdeII GATC 4 cut(s) 507, 578, 642, 710
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 2 cut(s) 191, 392
NlaIV GGNNCC 4 cut(s) 49, 334, 544, 712
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 3 cut(s) 29, 256, 364
NsiI ATGCAT 1 cut(s) 193
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PdiI GCCGGC 1 cut(s) 163
PflMI CCANNNNNTGG 2 cut(s) 389, 665
PkrI GCNGC 4 cut(s) 148, 223, 481, 559
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
Psp6I CCWGG 1 cut(s) 684
PspFI CCCAGC 2 cut(s) 250, 589
PspGI CCWGG 1 cut(s) 684
PspN4I GGNNCC 4 cut(s) 49, 334, 544, 712
PspPI GGNCC 4 cut(s) 184, 563, 605, 656
PstNI CAGNNNCTG 1 cut(s) 734
PsuI RGATCY 3 cut(s) 578, 642, 710
PvuII CAGCTG 1 cut(s) 254
SatI GCNGC 4 cut(s) 147, 222, 480, 558
Sau3AI GATC 4 cut(s) 507, 578, 642, 710
Sau96I GGNCC 4 cut(s) 184, 563, 605, 656
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 2 cut(s) 608, 686
SfaNI GCATC 1 cut(s) 133
SfcI CTRYAG 1 cut(s) 138
SinI GGWCC 2 cut(s) 563, 656
SmlI CTYRAG 2 cut(s) 53, 356
SmoI CTYRAG 2 cut(s) 53, 356
SsiI CCGC 4 cut(s) 480, 557, 573, 621
SspMI CTAG 3 cut(s) 65, 167, 554
StyD4I CCNGG 2 cut(s) 606, 684
TaaI ACNGT 1 cut(s) 466
TauI GCSGC 2 cut(s) 482, 560
TscAI CASTG 2 cut(s) 46, 469
TseFI GTSAC 3 cut(s) 29, 256, 364
TseI GCWGC 2 cut(s) 146, 221
Tsp45I GTSAC 3 cut(s) 29, 256, 364
TspDTI ATGAA 4 cut(s) 64, 523, 599, 694
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 2 cut(s) 46, 469
Van91I CCANNNNNTGG 2 cut(s) 389, 665
VpaK11BI GGWCC 2 cut(s) 563, 656
XmiI GTMKAC 1 cut(s) 459
XspI CTAG 3 cut(s) 65, 167, 554
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.