Rorug01G0109400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003279
Physical Location & Seq
Reverse (-)
19615172 .. 19617610
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0109400.1

Sequence Viewer

Length: 420 bp
ATGGATAAGACATGGGTTAAGCTACACAAAAAAGGCACCGAATATAAGGTCGGACTACAAAATTTCATCAGAAACTCCCTCCATGTGGTCTCCCACGAAGGGAGGATTAAATGCCCTTGTCAATCATGTGCTAATCATTCTTGGAGGAGCCCTGGTCTGGTATACAAACACATGCAAGCTGAGAGTATGTCTCCACACTATGAAGATGGATTGTGGGATGAACATGGTGAAGCTTTACCCAATCCTGTATTCTTGGATGAAATCGAAGAAGAAATTGAGGACCAACCTGACTTCAATCTAGACATGAATGATATGTTGACTGATGCATTTGCCAATAACAGTCCACCTAGACCTGTAAATGGACCGGATTTTGATCCGGACCCGCTCCAGATCCTTGGCTATTGGACGGGTTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.94

Weight (kDa)

4.73

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transpos_assoc PF13963 3 - 77 4.8e-14 Transposase-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 35
AccBSI CCGCTC 1 cut(s) 385
AccI GTMKAC 1 cut(s) 162
AccIII TCCGGA 1 cut(s) 376
AciI CCGC 1 cut(s) 383
AclWI GGATC 2 cut(s) 368, 385
AcsI RAATTY 1 cut(s) 61
AfiI CCNNNNNNNGG 4 cut(s) 85, 99, 157, 359
AgsI TTSAA 1 cut(s) 295
AjnI CCWGG 1 cut(s) 151
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 3 cut(s) 22, 179, 233
AluI AGCT 3 cut(s) 22, 179, 233
Alw26I GTCTC 2 cut(s) 94, 195
AlwI GGATC 2 cut(s) 368, 385
Aor13HI TCCGGA 1 cut(s) 376
ApoI RAATTY 1 cut(s) 61
ArsI GACNNNNNNTTYG 2 cut(s) 33, 65
AspS9I GGNCC 3 cut(s) 280, 362, 379
AsuHPI GGTGA 1 cut(s) 239
AvaII GGWCC 3 cut(s) 280, 362, 379
BanI GGYRCC 1 cut(s) 35
BanII GRGCYC 1 cut(s) 152
BccI CCATC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 153
BcoDI GTCTC 2 cut(s) 94, 195
BfaI CTAG 2 cut(s) 299, 348
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 3 cut(s) 280, 362, 379
BmgT120I GGNCC 3 cut(s) 280, 362, 379
BmiI GGNNCC 3 cut(s) 37, 149, 381
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 1 cut(s) 313
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 372
BsaI GGTCTC 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 151, 394
BsaWI WCCGGW 2 cut(s) 364, 376
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 99, 157, 359
Bse8I GATNNNNATC 1 cut(s) 372
BseAI TCCGGA 1 cut(s) 376
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 2 cut(s) 151, 394
BseGI GGATG 2 cut(s) 223, 262
BseJI GATNNNNATC 1 cut(s) 372
BseLI CCNNNNNNNGG 4 cut(s) 85, 99, 157, 359
BseMII CTCAG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 160
BshNI GGYRCC 1 cut(s) 35
BsiSI CCGG 2 cut(s) 365, 377
BslI CCNNNNNNNGG 4 cut(s) 85, 99, 157, 359
BsmAI GTCTC 2 cut(s) 94, 195
Bso31I GGTCTC 1 cut(s) 94
Bsp1286I GDGCHC 1 cut(s) 152
Bsp13I TCCGGA 1 cut(s) 376
Bsp143I GATC 2 cut(s) 373, 390
BspACI CCGC 1 cut(s) 383
BspCNI CTCAG 1 cut(s) 172
BspEI TCCGGA 1 cut(s) 376
BspLI GGNNCC 3 cut(s) 37, 149, 381
BspPI GGATC 2 cut(s) 368, 385
BspT107I GGYRCC 1 cut(s) 35
BspTNI GGTCTC 1 cut(s) 94
BsrBI CCGCTC 1 cut(s) 385
BssECI CCNNGG 2 cut(s) 151, 394
BssMI GATC 2 cut(s) 373, 390
BssNAI GTATAC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 394
Bst1107I GTATAC 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 177
BstDEI CTNAG 1 cut(s) 180
BstF5I GGATG 2 cut(s) 223, 262
BstKTI GATC 2 cut(s) 376, 393
BstMAI GTCTC 2 cut(s) 94, 195
BstMBI GATC 2 cut(s) 373, 390
BstNI CCWGG 1 cut(s) 153
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 390
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 1 cut(s) 390
BstZ17I GTATAC 1 cut(s) 163
BtsCI GGATG 2 cut(s) 223, 262
Cac8I GCNNGC 1 cut(s) 177
Cfr13I GGNCC 3 cut(s) 280, 362, 379
CviAII CATG 6 cut(s) 12, 83, 126, 172, 224, 304
CviJI RGCY 5 cut(s) 22, 150, 179, 233, 399
CviKI_1 RGCY 5 cut(s) 22, 150, 179, 233, 399
DdeI CTNAG 1 cut(s) 180
DpnI GATC 2 cut(s) 375, 392
DpnII GATC 2 cut(s) 373, 390
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 152
Eco31I GGTCTC 1 cut(s) 94
Eco47I GGWCC 3 cut(s) 280, 362, 379
EcoRII CCWGG 1 cut(s) 151
EcoT14I CCWWGG 1 cut(s) 394
EcoT22I ATGCAT 1 cut(s) 328
EcoT38I GRGCYC 1 cut(s) 152
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 6 cut(s) 15, 86, 129, 175, 227, 307
FatI CATG 6 cut(s) 11, 82, 125, 171, 223, 303
FauI CCCGC 1 cut(s) 390
FblI GTMKAC 1 cut(s) 162
FokI GGATG 2 cut(s) 230, 269
FriOI GRGCYC 1 cut(s) 152
FspBI CTAG 2 cut(s) 299, 348
GsuI CTGGAG 1 cut(s) 371
HapII CCGG 2 cut(s) 365, 377
Hin1II CATG 6 cut(s) 15, 86, 129, 175, 227, 307
HincII GTYRAC 1 cut(s) 318
HindII GTYRAC 1 cut(s) 318
HindIII AAGCTT 1 cut(s) 231
HpaII CCGG 2 cut(s) 365, 377
HphI GGTGA 1 cut(s) 239
Hpy166II GTNNAC 3 cut(s) 163, 318, 344
Hpy188I TCNGA 2 cut(s) 53, 71
Hpy188III TCNNGA 3 cut(s) 299, 377, 388
Hpy8I GTNNAC 3 cut(s) 163, 318, 344
HpyAV CCTTC 1 cut(s) 92
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4V TGCA 2 cut(s) 175, 326
HpyF3I CTNAG 1 cut(s) 180
Hsp92II CATG 6 cut(s) 15, 86, 129, 175, 227, 307
Kpn2I TCCGGA 1 cut(s) 376
Kzo9I GATC 2 cut(s) 373, 390
LmnI GCTCC 2 cut(s) 147, 390
LpnPI CCDG 9 cut(s) 138, 143, 165, 258, 300, 366, 378, 390, 401
LweI GCATC 1 cut(s) 313
MaeI CTAG 2 cut(s) 299, 348
MalI GATC 2 cut(s) 375, 392
MbiI CCGCTC 1 cut(s) 385
MboI GATC 2 cut(s) 373, 390
MboII GAAGA 3 cut(s) 215, 278, 281
MflI RGATCY 1 cut(s) 390
MhlI GDGCHC 1 cut(s) 152
MluCI AATT 2 cut(s) 61, 273
MmeI TCCRAC 1 cut(s) 31
MnlI CCTC 4 cut(s) 89, 96, 138, 271
Mph1103I ATGCAT 1 cut(s) 328
MroI TCCGGA 1 cut(s) 376
MseI TTAA 2 cut(s) 18, 108
MspI CCGG 2 cut(s) 365, 377
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
NdeII GATC 2 cut(s) 373, 390
NlaIII CATG 6 cut(s) 15, 86, 129, 175, 227, 307
NlaIV GGNNCC 3 cut(s) 37, 149, 381
NsiI ATGCAT 1 cut(s) 328
NspI RCATGY 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 3 cut(s) 37, 149, 381
PspPI GGNCC 3 cut(s) 280, 362, 379
PsuI RGATCY 1 cut(s) 390
SaqAI TTAA 2 cut(s) 18, 108
Sau3AI GATC 2 cut(s) 373, 390
Sau96I GGNCC 3 cut(s) 280, 362, 379
ScrFI CCNGG 1 cut(s) 153
SduI GDGCHC 1 cut(s) 152
SetI ASST 7 cut(s) 24, 51, 181, 235, 289, 349, 355
SfaNI GCATC 1 cut(s) 313
SinI GGWCC 3 cut(s) 280, 362, 379
Sse9I AATT 2 cut(s) 61, 273
SsiI CCGC 1 cut(s) 383
SspMI CTAG 2 cut(s) 299, 348
StyD4I CCNGG 1 cut(s) 151
StyI CCWWGG 1 cut(s) 394
TaaI ACNGT 1 cut(s) 341
TaqI TCGA 1 cut(s) 264
TasI AATT 2 cut(s) 61, 273
Tru1I TTAA 2 cut(s) 18, 108
Tru9I TTAA 2 cut(s) 18, 108
TspDTI ATGAA 5 cut(s) 55, 216, 234, 273, 320
VpaK11BI GGWCC 3 cut(s) 280, 362, 379
XapI RAATTY 1 cut(s) 61
XbaI TCTAGA 1 cut(s) 298
XceI RCATGY 1 cut(s) 175
XmiI GTMKAC 1 cut(s) 162
XspI CTAG 2 cut(s) 299, 348
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.