Rmu_ssc0000361.1_g000004

Belongs to the universal ribosomal protein uL5 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000361.1
OG0004258
Physical Location & Seq
Reverse (-)
28690 .. 32761
4072 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 789 bp
atggcccgggacatcactaaccttgtggttaactatctcagcctgcttgcggtgtttgatttatcatatggagagtcaccttggtctggggcttttctttcttcctcggtcgctgatttgggttgctttccggctcttcaattggaggatggtgttatggtggatggtttggtcttcggactctccgaggagcatctgctatggctgggttcgaatttagggcacagtttaatggtagatccatcggtgcatgttggggattggccggaagatttcgctagttctcaacaggggctaaccagggaaggcggaggtcgggggttgagatctattctggtcggtgacttgggtgaggctggtcgtggagatctgatcctacttgcttctgataacggttgggatgatgtcgctcagggacacggtggtgaaggcttctcgacggtggtgtgtgctcttggtggcgaaggaattgcagtggcggtgagggcttacggtgctggtggccggaaactatttggaggtggtgttggggtgcccatagcaacttccatgcattgggctctttgggccttaatctgggcgtcggagaaccccatgaggaaaatcaaggtccagaagctcatcctcaacatctctgtcggcgaaagcggcgatcgtctcaccaaggccgccaaggtcttggagcaactcagcttgaagactagccctactatcgaagaaatcaagaacatccttggtgccgagaagatggagctgctgggattgttaatgatgggtctgagcctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L5 Ribosomal_L5_C

Protein Analysis

262

Amino Acids

27.6

Weight (kDa)

4.78

Isoelectric Point (pI)

36.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L5 PF00281 197 - 233 5.3e-09 Ribosomal protein L5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 532, 737
AccB7I CCANNNNNTGG 1 cut(s) 555
AciI CCGC 5 cut(s) 50, 309, 479, 648, 669
AclWI GGATC 2 cut(s) 233, 367
AcoI YGGCCR 2 cut(s) 263, 502
AcsI RAATTY 1 cut(s) 214
AcyI GRCGYC 1 cut(s) 581
AfiI CCNNNNNNNGG 4 cut(s) 49, 86, 555, 576
AgsI TTSAA 2 cut(s) 140, 697
AjnI CCWGG 1 cut(s) 299
AleI CACNNNNGTG 1 cut(s) 423
AluBI AGCT 3 cut(s) 619, 693, 754
AluI AGCT 3 cut(s) 619, 693, 754
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 1 cut(s) 662
AlwI GGATC 2 cut(s) 233, 367
Ama87I CYCGRG 1 cut(s) 6
AoxI GGCC 5 cut(s) 3, 263, 502, 567, 666
ApeKI GCWGC 1 cut(s) 754
ApoI RAATTY 1 cut(s) 214
AspS9I GGNCC 3 cut(s) 4, 567, 610
AsuC2I CCSGG 2 cut(s) 7, 8
AsuHPI GGTGA 6 cut(s) 69, 353, 362, 437, 493, 652
AsuII TTCGAA 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 6
AvaII GGWCC 1 cut(s) 610
BaeGI GKGCMC 2 cut(s) 225, 537
BanI GGYRCC 2 cut(s) 532, 737
BanII GRGCYC 1 cut(s) 562
BbsI GAAGAC 2 cut(s) 166, 704
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 1 cut(s) 741
BccI CCATC 5 cut(s) 143, 158, 250, 742, 766
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BciT130I CCWGG 1 cut(s) 301
BcnI CCSGG 2 cut(s) 7, 8
BcoDI GTCTC 1 cut(s) 662
BfaI CTAG 2 cut(s) 279, 702
BglII AGATCT 2 cut(s) 326, 367
BisI GCNGC 3 cut(s) 649, 669, 755
BlsI GCNGC 3 cut(s) 650, 670, 756
Bme1390I CCNGG 3 cut(s) 7, 8, 301
Bme18I GGWCC 1 cut(s) 610
BmeT110I CYCGRG 1 cut(s) 6
BmgT120I GGNCC 3 cut(s) 4, 567, 610
BmiI GGNNCC 2 cut(s) 534, 739
BmrFI CCNGG 3 cut(s) 7, 8, 301
BmsI GCATC 1 cut(s) 202
BpiI GAAGAC 2 cut(s) 166, 704
Bpu10I CCTNAGC 1 cut(s) 411
Bpu14I TTCGAA 1 cut(s) 212
BpuMI CCSGG 2 cut(s) 7, 8
BsaHI GRCGYC 1 cut(s) 581
BsaJI CCNNGG 8 cut(s) 6, 80, 105, 186, 300, 663, 672, 733
BsaXI ACNNNNNCTCC 2 cut(s) 510, 540
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 86, 555, 576
BseBI CCWGG 1 cut(s) 301
BseDI CCNNGG 8 cut(s) 6, 80, 105, 186, 300, 663, 672, 733
BseGI GGATG 5 cut(s) 154, 169, 406, 621, 729
BseLI CCNNNNNNNGG 4 cut(s) 49, 86, 555, 576
BseMII CTCAG 4 cut(s) 52, 425, 703, 770
BseRI GAGGAG 1 cut(s) 203
BseSI GKGCMC 2 cut(s) 225, 537
BseXI GCAGC 1 cut(s) 741
BseYI CCCAGC 2 cut(s) 205, 757
Bsh1285I CGRYCG 2 cut(s) 111, 655
BshFI GGCC 5 cut(s) 5, 265, 504, 569, 668
BshNI GGYRCC 2 cut(s) 532, 737
BsiEI CGRYCG 2 cut(s) 111, 655
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 1 cut(s) 6
BsiSI CCGG 4 cut(s) 7, 131, 266, 505
BslFI GGGAC 2 cut(s) 23, 429
BslI CCNNNNNNNGG 4 cut(s) 49, 86, 555, 576
BsmAI GTCTC 1 cut(s) 662
BsmBI CGTCTC 1 cut(s) 662
BsmFI GGGAC 2 cut(s) 23, 429
BsnI GGCC 5 cut(s) 5, 265, 504, 569, 668
BsoBI CYCGRG 1 cut(s) 6
Bsp119I TTCGAA 1 cut(s) 212
Bsp1286I GDGCHC 4 cut(s) 225, 454, 537, 562
Bsp143I GATC 5 cut(s) 238, 326, 367, 372, 652
BspACI CCGC 5 cut(s) 50, 309, 479, 648, 669
BspANI GGCC 5 cut(s) 5, 265, 504, 569, 668
BspCNI CTCAG 4 cut(s) 51, 424, 702, 771
BspLI GGNNCC 2 cut(s) 534, 739
BspPI GGATC 2 cut(s) 233, 367
BspQI GCTCTTC 1 cut(s) 141
BspT104I TTCGAA 1 cut(s) 212
BspT107I GGYRCC 2 cut(s) 532, 737
BssECI CCNNGG 8 cut(s) 6, 80, 105, 186, 300, 663, 672, 733
BssMI GATC 5 cut(s) 238, 326, 367, 372, 652
BssNI GRCGYC 1 cut(s) 581
BssT1I CCWWGG 4 cut(s) 80, 663, 672, 733
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 5 cut(s) 227, 395, 422, 442, 494
Bst6I CTCTTC 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 581
BstBI TTCGAA 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 44, 48
BstDEI CTNAG 4 cut(s) 38, 411, 689, 779
BstF5I GGATG 5 cut(s) 154, 169, 406, 621, 729
BstKTI GATC 5 cut(s) 241, 329, 370, 375, 655
BstMAI GTCTC 1 cut(s) 662
BstMBI GATC 5 cut(s) 238, 326, 367, 372, 652
BstMCI CGRYCG 2 cut(s) 111, 655
BstMWI GCNNNNNNNGC 4 cut(s) 485, 494, 566, 648
BstNI CCWGG 1 cut(s) 301
BstNSI RCATGY 1 cut(s) 254
BstSCI CCNGG 3 cut(s) 5, 6, 299
BstSLI GKGCMC 2 cut(s) 225, 537
BstV1I GCAGC 1 cut(s) 741
BstV2I GAAGAC 2 cut(s) 166, 704
BstX2I RGATCY 3 cut(s) 238, 326, 367
BstXI CCANNNNNNTGG 1 cut(s) 679
BstYI RGATCY 3 cut(s) 238, 326, 367
BsuRI GGCC 5 cut(s) 5, 265, 504, 569, 668
BtsCI GGATG 5 cut(s) 154, 169, 406, 621, 729
BtsI GCAGTG 1 cut(s) 480
BtsIMutI CAGTG 1 cut(s) 480
Cac8I GCNNGC 2 cut(s) 44, 48
Cfr13I GGNCC 3 cut(s) 4, 567, 610
Cfr9I CCCGGG 1 cut(s) 6
CseI GACGC 1 cut(s) 570
CviAII CATG 3 cut(s) 251, 550, 595
DdeI CTNAG 4 cut(s) 38, 411, 689, 779
DpnI GATC 5 cut(s) 240, 328, 369, 374, 654
DpnII GATC 5 cut(s) 238, 326, 367, 372, 652
EaeI YGGCCR 2 cut(s) 263, 502
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 324
Eco130I CCWWGG 4 cut(s) 80, 663, 672, 733
Eco24I GRGCYC 1 cut(s) 562
Eco47I GGWCC 1 cut(s) 610
Eco88I CYCGRG 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 4 cut(s) 80, 663, 672, 733
EcoT22I ATGCAT 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 562
ErhI CCWWGG 4 cut(s) 80, 663, 672, 733
Esp3I CGTCTC 1 cut(s) 662
FaeI CATG 3 cut(s) 254, 553, 598
FaiI YATR 8 cut(s) 67, 69, 158, 202, 252, 539, 551, 596
FaqI GGGAC 2 cut(s) 23, 429
FatI CATG 3 cut(s) 250, 549, 594
FauNDI CATATG 1 cut(s) 67
Fnu4HI GCNGC 3 cut(s) 649, 669, 755
FokI GGATG 5 cut(s) 161, 176, 413, 608, 716
FriOI GRGCYC 1 cut(s) 562
Fsp4HI GCNGC 3 cut(s) 649, 669, 755
FspBI CTAG 2 cut(s) 279, 702
GluI GCNGC 3 cut(s) 649, 669, 755
GsaI CCCAGC 2 cut(s) 209, 761
HaeIII GGCC 5 cut(s) 5, 265, 504, 569, 668
HapII CCGG 4 cut(s) 7, 131, 266, 505
HgaI GACGC 1 cut(s) 570
Hin1I GRCGYC 1 cut(s) 581
Hin1II CATG 3 cut(s) 254, 553, 598
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 2 cut(s) 74, 180
HpaI GTTAAC 1 cut(s) 31
HpaII CCGG 4 cut(s) 7, 131, 266, 505
HphI GGTGA 6 cut(s) 69, 353, 362, 437, 493, 652
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 7 cut(s) 179, 187, 372, 388, 586, 780, 788
Hpy188III TCNNGA 3 cut(s) 436, 613, 724
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 2 cut(s) 442, 586
HpyAV CCTTC 3 cut(s) 299, 422, 458
HpyCH4III ACNGT 5 cut(s) 227, 395, 422, 442, 494
HpyCH4V TGCA 3 cut(s) 250, 473, 553
HpyF10VI GCNNNNNNNGC 4 cut(s) 485, 494, 566, 648
HpyF3I CTNAG 4 cut(s) 38, 411, 689, 779
Hsp92I GRCGYC 1 cut(s) 581
Hsp92II CATG 3 cut(s) 254, 553, 598
KspAI GTTAAC 1 cut(s) 31
Kzo9I GATC 5 cut(s) 238, 326, 367, 372, 652
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 3 cut(s) 190, 682, 751
Lsp1109I GCAGC 1 cut(s) 741
LweI GCATC 1 cut(s) 202
MaeI CTAG 2 cut(s) 279, 702
MaeIII GTNAC 2 cut(s) 75, 341
MalI GATC 5 cut(s) 240, 328, 369, 374, 654
MboI GATC 5 cut(s) 238, 326, 367, 372, 652
MboII GAAGA 7 cut(s) 93, 128, 166, 281, 709, 728, 757
MfeI CAATTG 1 cut(s) 140
MflI RGATCY 3 cut(s) 238, 326, 367
MhlI GDGCHC 4 cut(s) 225, 454, 537, 562
MluCI AATT 3 cut(s) 140, 214, 468
MlyI GAGTC 2 cut(s) 83, 174
MmeI TCCRAC 1 cut(s) 564
MnlI CCTC 9 cut(s) 115, 139, 181, 305, 346, 477, 512, 591, 635
Mph1103I ATGCAT 1 cut(s) 555
MseI TTAA 4 cut(s) 30, 230, 572, 767
MslI CAYNNNNRTG 1 cut(s) 423
MspI CCGG 4 cut(s) 7, 131, 266, 505
MspR9I CCNGG 3 cut(s) 7, 8, 301
MunI CAATTG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 301
MwoI GCNNNNNNNGC 4 cut(s) 485, 494, 566, 648
NciI CCSGG 2 cut(s) 7, 8
NdeI CATATG 1 cut(s) 67
NdeII GATC 5 cut(s) 238, 326, 367, 372, 652
NlaIII CATG 3 cut(s) 254, 553, 598
NlaIV GGNNCC 2 cut(s) 534, 739
NmeAIII GCCGAG 1 cut(s) 766
NmuCI GTSAC 2 cut(s) 75, 341
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 1 cut(s) 254
NspV TTCGAA 1 cut(s) 212
OliI CACNNNNGTG 1 cut(s) 423
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PflMI CCANNNNNTGG 1 cut(s) 555
PkrI GCNGC 3 cut(s) 650, 670, 756
Ple19I CGATCG 1 cut(s) 655
PleI GAGTC 2 cut(s) 82, 174
PpsI GAGTC 2 cut(s) 82, 174
Psp6I CCWGG 1 cut(s) 299
PspFI CCCAGC 2 cut(s) 205, 757
PspGI CCWGG 1 cut(s) 299
PspN4I GGNNCC 2 cut(s) 534, 739
PspPI GGNCC 3 cut(s) 4, 567, 610
PsuI RGATCY 3 cut(s) 238, 326, 367
PvuI CGATCG 1 cut(s) 655
RseI CAYNNNNRTG 1 cut(s) 423
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 4 cut(s) 30, 230, 572, 767
SatI GCNGC 3 cut(s) 649, 669, 755
Sau3AI GATC 5 cut(s) 238, 326, 367, 372, 652
Sau96I GGNCC 3 cut(s) 4, 567, 610
SchI GAGTC 2 cut(s) 83, 174
ScrFI CCNGG 3 cut(s) 7, 8, 301
SduI GDGCHC 4 cut(s) 225, 454, 537, 562
SetI ASST 9 cut(s) 24, 82, 316, 523, 612, 621, 678, 695, 756
SfaNI GCATC 1 cut(s) 202
SfuI TTCGAA 1 cut(s) 212
SinI GGWCC 1 cut(s) 610
SmaI CCCGGG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 423
Sse9I AATT 3 cut(s) 140, 214, 468
SsiI CCGC 5 cut(s) 50, 309, 479, 648, 669
SspMI CTAG 2 cut(s) 279, 702
StyD4I CCNGG 3 cut(s) 5, 6, 299
StyI CCWWGG 4 cut(s) 80, 663, 672, 733
TaaI ACNGT 5 cut(s) 227, 395, 422, 442, 494
TaqI TCGA 3 cut(s) 212, 437, 714
TaqII GACCGA 1 cut(s) 97
TasI AATT 3 cut(s) 140, 214, 468
TauI GCSGC 2 cut(s) 651, 671
Tru1I TTAA 4 cut(s) 30, 230, 572, 767
Tru9I TTAA 4 cut(s) 30, 230, 572, 767
TscAI CASTG 1 cut(s) 480
TseFI GTSAC 2 cut(s) 75, 341
TseI GCWGC 1 cut(s) 754
Tsp45I GTSAC 2 cut(s) 75, 341
TspMI CCCGGG 1 cut(s) 6
TspRI CASTG 1 cut(s) 480
Van91I CCANNNNNTGG 1 cut(s) 555
VpaK11BI GGWCC 1 cut(s) 610
XapI RAATTY 1 cut(s) 214
XceI RCATGY 1 cut(s) 254
XmaI CCCGGG 1 cut(s) 6
XspI CTAG 2 cut(s) 279, 702
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.