Rh2BG001100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004258
Physical Location & Seq
Reverse (-)
147403 .. 166702
19300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG001100.1

Sequence Viewer

Length: 732 bp
ATGGCCATGGTCATCATCAGCCTTGCGGCTCTCTGTTTCTTGCTGCTTGCTGTGTTTGATTGTTCATATGGGAAGCCACCTTGGCCTGGGGCTTCTCTTTCTCCTTTTGCCGTCAACCAAGTGTTCCGGTTTCGAAGGGAGTCGATCAAAGACGGCGGAGGTCTGGGGTTGATTCTACTGCGATCTGGCTGGGCCGATAACGGGGGCGCGATTGGTTGGGGAAATCTGACCCGATTTGCTTCTGCTGGTGGTTGGGACGGAGTCTCACGGGGACAGGATGGTGGCGGCTTCTCGGCGGGGGTGCGAGAGTTTGGTGGCGACGGCCTTGCAGGGACAGAGCTGGCTCATGGTTTCGGTGGCCGGAATCTGTTGGGTGGAAGTGTTGGGGTGCCCATCGTAGTCTCCATGCTTTGGGCCTCTTTCTGGGCCGGTGAATACAGATCATCTTTCACTGATCATCAGAGAAGAAGACAGAGCCTTTTGTGGCTGAAATGCAACAAGCGCACAAGGAGACCTCTCTTTCTTGCTGCCTCATTTGGAAATTCTCTTACCAAGATCACCCAAATCAAGCACGAAACAACAACAGTTGGCTTATTGGGGACGGCGTCTCATGGTTTGTTCATTGAAGACCGACCTGAGGAATTAGCTGGTGACGTTTTCTTTCGATTTGGGGTTTGGATCTGGTTCACGTGTTTCGTATCTTATAAGTTTGGGCGTGCTTTTGGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

26.34

Weight (kDa)

9.74

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AccB1I GGYRCC 1 cut(s) 388
AccB7I CCANNNNNTGG 1 cut(s) 411
AccII CGCG 1 cut(s) 209
AciI CCGC 4 cut(s) 26, 156, 285, 296
AclWI GGATC 1 cut(s) 686
AcoI YGGCCR 2 cut(s) 3, 358
AcsI RAATTY 1 cut(s) 541
AcvI CACGTG 1 cut(s) 690
AcyI GRCGYC 1 cut(s) 605
AfiI CCNNNNNNNGG 5 cut(s) 86, 201, 411, 423, 637
AflIII ACRYGT 1 cut(s) 689
AgsI TTSAA 1 cut(s) 626
AjnI CCWGG 1 cut(s) 85
AluBI AGCT 2 cut(s) 340, 647
AluI AGCT 2 cut(s) 340, 647
Alw26I GTCTC 4 cut(s) 268, 406, 505, 612
AlwI GGATC 1 cut(s) 686
AoxI GGCC 7 cut(s) 3, 83, 192, 322, 358, 414, 426
ApeKI GCWGC 2 cut(s) 43, 527
ApoI RAATTY 1 cut(s) 541
AspLEI GCGC 2 cut(s) 209, 504
AspS9I GGNCC 3 cut(s) 192, 414, 426
AsuHPI GGTGA 3 cut(s) 443, 550, 662
AsuII TTCGAA 1 cut(s) 133
AxyI CCTNAGG 1 cut(s) 636
BaeGI GKGCMC 1 cut(s) 393
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 388
BbrPI CACGTG 1 cut(s) 690
BbsI GAAGAC 2 cut(s) 475, 633
BbvI GCAGC 2 cut(s) 30, 514
BccI CCATC 2 cut(s) 272, 401
BceAI ACGGC 4 cut(s) 95, 169, 337, 618
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 87
BclI TGATCA 1 cut(s) 454
BcoDI GTCTC 4 cut(s) 268, 406, 505, 612
BglI GCCNNNNNGGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 27, 44, 286, 528
BlsI GCNGC 4 cut(s) 28, 45, 287, 529
Bme1390I CCNGG 1 cut(s) 87
BmgT120I GGNCC 3 cut(s) 192, 414, 426
BmiI GGNNCC 1 cut(s) 390
BmrFI CCNGG 1 cut(s) 87
BpiI GAAGAC 2 cut(s) 475, 633
Bpu14I TTCGAA 1 cut(s) 133
BsaAI YACGTR 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 605
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 3 cut(s) 6, 80, 86
BsaWI WCCGGW 1 cut(s) 126
Bsc4I CCNNNNNNNGG 5 cut(s) 86, 201, 411, 423, 637
Bse118I RCCGGY 1 cut(s) 428
Bse21I CCTNAGG 1 cut(s) 636
BseBI CCWGG 1 cut(s) 87
BseDI CCNNGG 3 cut(s) 6, 80, 86
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 5 cut(s) 86, 201, 411, 423, 637
BseMII CTCAG 1 cut(s) 627
BseSI GKGCMC 1 cut(s) 393
BseXI GCAGC 2 cut(s) 30, 514
BseYI CCCAGC 1 cut(s) 189
Bsh1236I CGCG 1 cut(s) 209
BshFI GGCC 7 cut(s) 5, 85, 194, 324, 360, 416, 428
BshNI GGYRCC 1 cut(s) 388
BsiSI CCGG 3 cut(s) 127, 361, 429
BslFI GGGAC 4 cut(s) 269, 285, 346, 613
BslI CCNNNNNNNGG 5 cut(s) 86, 201, 411, 423, 637
BsmAI GTCTC 4 cut(s) 268, 406, 505, 612
BsmBI CGTCTC 1 cut(s) 612
BsmFI GGGAC 4 cut(s) 269, 285, 346, 613
BsnI GGCC 7 cut(s) 5, 85, 194, 324, 360, 416, 428
Bso31I GGTCTC 1 cut(s) 505
Bsp119I TTCGAA 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 393
Bsp143I GATC 6 cut(s) 144, 182, 440, 454, 555, 678
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 4 cut(s) 26, 156, 285, 296
BspANI GGCC 7 cut(s) 5, 85, 194, 324, 360, 416, 428
BspCNI CTCAG 1 cut(s) 628
BspFNI CGCG 1 cut(s) 209
BspLI GGNNCC 1 cut(s) 390
BspPI GGATC 1 cut(s) 686
BspT104I TTCGAA 1 cut(s) 133
BspT107I GGYRCC 1 cut(s) 388
BspTNI GGTCTC 1 cut(s) 505
BsrFI RCCGGY 1 cut(s) 428
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 3 cut(s) 6, 80, 86
BssMI GATC 6 cut(s) 144, 182, 440, 454, 555, 678
BssNI GRCGYC 1 cut(s) 605
BssT1I CCWWGG 2 cut(s) 6, 80
Bst2UI CCWGG 1 cut(s) 87
Bst4CI ACNGT 1 cut(s) 586
BstACI GRCGYC 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 690
BstBI TTCGAA 1 cut(s) 133
BstC8I GCNNGC 3 cut(s) 48, 342, 717
BstDEI CTNAG 1 cut(s) 636
BstDSI CCRYGG 1 cut(s) 6
BstF5I GGATG 1 cut(s) 283
BstFNI CGCG 1 cut(s) 209
BstHHI GCGC 2 cut(s) 209, 504
BstKTI GATC 6 cut(s) 147, 185, 443, 457, 558, 681
BstMAI GTCTC 4 cut(s) 268, 406, 505, 612
BstMBI GATC 6 cut(s) 144, 182, 440, 454, 555, 678
BstMWI GCNNNNNNNGC 2 cut(s) 82, 501
BstNI CCWGG 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 85
BstSLI GKGCMC 1 cut(s) 393
BstUI CGCG 1 cut(s) 209
BstV1I GCAGC 2 cut(s) 30, 514
BstV2I GAAGAC 2 cut(s) 475, 633
BstX2I RGATCY 1 cut(s) 678
BstYI RGATCY 1 cut(s) 678
Bsu36I CCTNAGG 1 cut(s) 636
BsuRI GGCC 7 cut(s) 5, 85, 194, 324, 360, 416, 428
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 450
Cac8I GCNNGC 3 cut(s) 48, 342, 717
CfoI GCGC 2 cut(s) 209, 504
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 3 cut(s) 192, 414, 426
CseI GACGC 1 cut(s) 594
CviAII CATG 4 cut(s) 7, 347, 406, 611
DdeI CTNAG 1 cut(s) 636
DpnI GATC 6 cut(s) 146, 184, 442, 456, 557, 680
DpnII GATC 6 cut(s) 144, 182, 440, 454, 555, 678
EaeI YGGCCR 2 cut(s) 3, 358
EciI GGCGGA 1 cut(s) 171
Eco130I CCWWGG 2 cut(s) 6, 80
Eco31I GGTCTC 1 cut(s) 505
Eco72I CACGTG 1 cut(s) 690
Eco81I CCTNAGG 1 cut(s) 636
EcoRII CCWGG 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 6, 80
ErhI CCWWGG 2 cut(s) 6, 80
Esp3I CGTCTC 1 cut(s) 612
FaeI CATG 4 cut(s) 10, 350, 409, 614
FaiI YATR 7 cut(s) 8, 67, 69, 348, 407, 612, 705
FaqI GGGAC 4 cut(s) 269, 285, 346, 613
FatI CATG 4 cut(s) 6, 346, 405, 610
FauI CCCGC 1 cut(s) 289
FauNDI CATATG 1 cut(s) 67
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 4 cut(s) 27, 44, 286, 528
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 4 cut(s) 27, 44, 286, 528
GlaI GCGC 2 cut(s) 208, 503
GluI GCNGC 4 cut(s) 27, 44, 286, 528
GsaI CCCAGC 1 cut(s) 193
HaeIII GGCC 7 cut(s) 5, 85, 194, 324, 360, 416, 428
HapII CCGG 3 cut(s) 127, 361, 429
HgaI GACGC 1 cut(s) 594
HhaI GCGC 2 cut(s) 209, 504
Hin1I GRCGYC 1 cut(s) 605
Hin1II CATG 4 cut(s) 10, 350, 409, 614
Hin6I GCGC 2 cut(s) 207, 502
HinP1I GCGC 2 cut(s) 207, 502
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 4 cut(s) 140, 172, 261, 364
HpaII CCGG 3 cut(s) 127, 361, 429
HphI GGTGA 3 cut(s) 443, 550, 662
Hpy166II GTNNAC 2 cut(s) 115, 687
Hpy188I TCNGA 2 cut(s) 228, 462
Hpy8I GTNNAC 2 cut(s) 115, 687
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 1 cut(s) 129
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 2 cut(s) 654, 689
HpyCH4V TGCA 2 cut(s) 329, 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 501
HpyF3I CTNAG 1 cut(s) 636
HpySE526I ACGT 2 cut(s) 654, 689
Hsp92I GRCGYC 1 cut(s) 605
Hsp92II CATG 4 cut(s) 10, 350, 409, 614
HspAI GCGC 2 cut(s) 207, 502
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 6 cut(s) 144, 182, 440, 454, 555, 678
Lsp1109I GCAGC 2 cut(s) 30, 514
MaeII ACGT 2 cut(s) 654, 689
MaeIII GTNAC 1 cut(s) 650
MalI GATC 6 cut(s) 146, 184, 442, 456, 557, 680
MboI GATC 6 cut(s) 144, 182, 440, 454, 555, 678
MboII GAAGA 3 cut(s) 477, 480, 638
MflI RGATCY 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 393
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 541, 641
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 149, 270
MnlI CCTC 5 cut(s) 152, 427, 525, 541, 631
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 127, 361, 429
MspR9I CCNGG 1 cut(s) 87
MvaI CCWGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 209
MwoI GCNNNNNNNGC 2 cut(s) 82, 501
NcoI CCATGG 1 cut(s) 6
NdeI CATATG 1 cut(s) 67
NdeII GATC 6 cut(s) 144, 182, 440, 454, 555, 678
NlaIII CATG 4 cut(s) 10, 350, 409, 614
NlaIV GGNNCC 1 cut(s) 390
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 650
NspV TTCGAA 1 cut(s) 133
PfeI GAWTC 2 cut(s) 172, 364
PflFI GACNNNGTC 2 cut(s) 260, 604
PflMI CCANNNNNTGG 1 cut(s) 411
PkrI GCNGC 4 cut(s) 28, 45, 287, 529
PleI GAGTC 2 cut(s) 148, 269
PmaCI CACGTG 1 cut(s) 690
PmlI CACGTG 1 cut(s) 690
PpsI GAGTC 2 cut(s) 148, 269
Ppu21I YACGTR 1 cut(s) 690
PsiI TTATAA 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 85
PspCI CACGTG 1 cut(s) 690
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 1 cut(s) 85
PspN4I GGNNCC 1 cut(s) 390
PspPI GGNCC 3 cut(s) 192, 414, 426
PsuI RGATCY 1 cut(s) 678
PsyI GACNNNGTC 2 cut(s) 260, 604
SatI GCNGC 4 cut(s) 27, 44, 286, 528
Sau3AI GATC 6 cut(s) 144, 182, 440, 454, 555, 678
Sau96I GGNCC 3 cut(s) 192, 414, 426
SchI GAGTC 2 cut(s) 149, 270
ScrFI CCNGG 1 cut(s) 87
SduI GDGCHC 1 cut(s) 393
SetI ASST 8 cut(s) 82, 163, 342, 517, 637, 649, 657, 692
SfuI TTCGAA 1 cut(s) 133
Sse9I AATT 2 cut(s) 541, 641
SsiI CCGC 4 cut(s) 26, 156, 285, 296
StyD4I CCNGG 1 cut(s) 85
StyI CCWWGG 2 cut(s) 6, 80
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 2 cut(s) 657, 692
TaqI TCGA 3 cut(s) 133, 143, 664
TaqII GACCGA 1 cut(s) 645
TasI AATT 2 cut(s) 541, 641
TauI GCSGC 2 cut(s) 29, 288
TfiI GAWTC 2 cut(s) 172, 364
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 2 cut(s) 43, 527
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 2 cut(s) 54, 610
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 1 cut(s) 457
Tth111I GACNNNGTC 2 cut(s) 260, 604
Van91I CCANNNNNTGG 1 cut(s) 411
XapI RAATTY 1 cut(s) 541
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.