Rroxscaffold_2G00113320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003693
Physical Location & Seq
Reverse (-)
38875317 .. 38876338
1022 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00113320.1

Sequence Viewer

Length: 618 bp
ATGAACAAACAGCACAAAAACGCAAGAAAGATATATTGGAAGCACGGCAATATGAGAAAAGTTGGCGTTTACCCGACAAAAGAGTGTGATTGTCGTCGAAGCAACGTCGACGTTGCTTCGAAGACGATAGGCTGGCAAGAAGGATTTAGGAGTGAGGAAGAAGAGCTTCAATTCTTCACGGGAAAACACGCTGGGGCCAACAAAAGCATAGGGAAATGGAACGATGACATTTCTCACCCTCAAATAGGGAGGGAAATTTATTCCTCGTTCTTTCAAGTGTTGCGGGATGATTTTTCTAGAGAGGAGTTGTTTCGCCGCATCATTGAATTGGAGAGTAAATTTATAGTCTCTGAACACACTCCTGCCTTATTTACAAAAAGCTTCGGACTGAAGATCTCGTTGAAGAGTCGAGAGAAATCTATGGTAGGTGGAATGGACATAGCGAAAGTGAAGGCTCCGACACCGCAAGGGAGGAAAACCAAAAAACGAGAAAGTGTCTCCATCGTTGCTGAAAAGGCTCAAGCCGATTCTGTAACTATTGGAGCCAACTCCCGAGGTGAAAAAGAGCCCCCAAGGGCTCTCTCCACCCCCCAGCTTGATCCCCCCTACGTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

23.29

Weight (kDa)

9.67

Isoelectric Point (pI)

47.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 108
AciI CCGC 3 cut(s) 283, 316, 464
AclWI GGATC 1 cut(s) 593
AcsI RAATTY 2 cut(s) 255, 338
AcuI CTGAAG 1 cut(s) 410
AfiI CCNNNNNNNGG 1 cut(s) 245
AgsI TTSAA 4 cut(s) 170, 275, 326, 403
AjuI GAANNNNNNNTTGG 2 cut(s) 19, 51
AluBI AGCT 3 cut(s) 166, 381, 595
AluI AGCT 3 cut(s) 166, 381, 595
Alw26I GTCTC 2 cut(s) 352, 502
AlwI GGATC 1 cut(s) 593
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 1 cut(s) 195
ApoI RAATTY 2 cut(s) 255, 338
Asp700I GAANNNNTTC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 195
AsuHPI GGTGA 2 cut(s) 227, 569
AsuII TTCGAA 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 552
BanII GRGCYC 2 cut(s) 570, 580
BbsI GAAGAC 1 cut(s) 128
BccI CCATC 1 cut(s) 509
BceAI ACGGC 1 cut(s) 61
BcoDI GTCTC 2 cut(s) 352, 502
BfaI CTAG 1 cut(s) 297
BglII AGATCT 1 cut(s) 393
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 1 cut(s) 195
BmiI GGNNCC 3 cut(s) 196, 456, 544
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 128
Bpu14I TTCGAA 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 504
BsaAI YACGTR 1 cut(s) 610
BsaJI CCNNGG 2 cut(s) 553, 572
Bsc4I CCNNNNNNNGG 1 cut(s) 245
BseDI CCNNGG 2 cut(s) 553, 572
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 1 cut(s) 245
BseRI GAGGAG 1 cut(s) 317
BseYI CCCAGC 2 cut(s) 191, 591
BshFI GGCC 1 cut(s) 197
BsiHKCI CYCGRG 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 2 cut(s) 352, 502
BsnI GGCC 1 cut(s) 197
BsoBI CYCGRG 1 cut(s) 552
Bsp119I TTCGAA 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 570, 580
Bsp143I GATC 2 cut(s) 393, 598
BspACI CCGC 3 cut(s) 283, 316, 464
BspANI GGCC 1 cut(s) 197
BspLI GGNNCC 3 cut(s) 196, 456, 544
BspPI GGATC 1 cut(s) 593
BspQI GCTCTTC 1 cut(s) 156
BspT104I TTCGAA 1 cut(s) 119
BssECI CCNNGG 2 cut(s) 553, 572
BssMI GATC 2 cut(s) 393, 598
BssT1I CCWWGG 1 cut(s) 572
Bst6I CTCTTC 2 cut(s) 156, 398
BstBAI YACGTR 1 cut(s) 610
BstBI TTCGAA 1 cut(s) 119
BstC8I GCNNGC 1 cut(s) 134
BstENI CCTNNNNNAGG 1 cut(s) 243
BstF5I GGATG 1 cut(s) 292
BstKTI GATC 2 cut(s) 396, 601
BstMAI GTCTC 2 cut(s) 352, 502
BstMBI GATC 2 cut(s) 393, 598
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstSNI TACGTA 1 cut(s) 610
BstV2I GAAGAC 1 cut(s) 128
BstX2I RGATCY 1 cut(s) 393
BstYI RGATCY 1 cut(s) 393
BsuRI GGCC 1 cut(s) 197
BtsCI GGATG 1 cut(s) 292
Cac8I GCNNGC 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 195
DpnI GATC 2 cut(s) 395, 600
DpnII GATC 2 cut(s) 393, 598
Eam1104I CTCTTC 2 cut(s) 156, 398
EarI CTCTTC 2 cut(s) 156, 398
Eco105I TACGTA 1 cut(s) 610
Eco130I CCWWGG 1 cut(s) 572
Eco24I GRGCYC 2 cut(s) 570, 580
Eco57I CTGAAG 1 cut(s) 410
Eco88I CYCGRG 1 cut(s) 552
EcoNI CCTNNNNNAGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 572
EcoT38I GRGCYC 2 cut(s) 570, 580
ErhI CCWWGG 1 cut(s) 572
FaiI YATR 6 cut(s) 34, 53, 209, 344, 422, 440
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FauI CCCGC 1 cut(s) 276
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 1 cut(s) 299
FriOI GRGCYC 2 cut(s) 570, 580
Fsp4HI GCNGC 1 cut(s) 316
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 316
GsaI CCCAGC 2 cut(s) 195, 595
HaeIII GGCC 1 cut(s) 197
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 2 cut(s) 406, 527
HphI GGTGA 2 cut(s) 227, 569
Hpy166II GTNNAC 2 cut(s) 70, 109
Hpy188I TCNGA 3 cut(s) 352, 386, 459
Hpy188III TCNNGA 3 cut(s) 297, 410, 552
Hpy8I GTNNAC 2 cut(s) 70, 109
Hpy99I CGWCG 3 cut(s) 99, 110, 113
HpyAV CCTTC 2 cut(s) 134, 445
HpyCH4IV ACGT 3 cut(s) 105, 111, 609
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpySE526I ACGT 3 cut(s) 105, 111, 609
Kzo9I GATC 2 cut(s) 393, 598
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 2 cut(s) 460, 542
LpnPI CCDG 4 cut(s) 118, 177, 375, 605
LweI GCATC 1 cut(s) 327
MaeI CTAG 1 cut(s) 297
MaeII ACGT 3 cut(s) 105, 111, 609
MaeIII GTNAC 1 cut(s) 532
MalI GATC 2 cut(s) 395, 600
MboI GATC 2 cut(s) 393, 598
MboII GAAGA 6 cut(s) 133, 166, 170, 173, 403, 415
MflI RGATCY 1 cut(s) 393
MhlI GDGCHC 2 cut(s) 570, 580
MluCI AATT 4 cut(s) 170, 255, 326, 338
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 1 cut(s) 482
MnlI CCTC 7 cut(s) 148, 243, 249, 274, 295, 465, 548
MroXI GAANNNNTTC 1 cut(s) 165
MseI TTAA 1 cut(s) 616
MwoI GCNNNNNNNGC 1 cut(s) 515
NdeII GATC 2 cut(s) 393, 598
NlaIV GGNNCC 3 cut(s) 196, 456, 544
NspV TTCGAA 1 cut(s) 119
PciSI GCTCTTC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 165
PfeI GAWTC 1 cut(s) 527
PkrI GCNGC 1 cut(s) 317
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Ppu21I YACGTR 1 cut(s) 610
PspFI CCCAGC 2 cut(s) 191, 591
PspN4I GGNNCC 3 cut(s) 196, 456, 544
PspPI GGNCC 1 cut(s) 195
PsuI RGATCY 1 cut(s) 393
SalI GTCGAC 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 1 cut(s) 616
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 2 cut(s) 393, 598
Sau96I GGNCC 1 cut(s) 195
SchI GAGTC 1 cut(s) 415
SduI GDGCHC 2 cut(s) 570, 580
SetI ASST 8 cut(s) 108, 114, 168, 383, 430, 559, 597, 612
SfaNI GCATC 1 cut(s) 327
SfuI TTCGAA 1 cut(s) 119
SgrDI CGTCGACG 1 cut(s) 107
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
SnaBI TACGTA 1 cut(s) 610
Sse9I AATT 4 cut(s) 170, 255, 326, 338
SsiI CCGC 3 cut(s) 283, 316, 464
SspMI CTAG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 572
TaiI ACGT 3 cut(s) 108, 114, 612
TaqI TCGA 4 cut(s) 97, 108, 119, 409
TasI AATT 4 cut(s) 170, 255, 326, 338
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 1 cut(s) 616
Tru9I TTAA 1 cut(s) 616
TspDTI ATGAA 1 cut(s) 17
XagI CCTNNNNNAGG 1 cut(s) 243
XapI RAATTY 2 cut(s) 255, 338
XbaI TCTAGA 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 108
XmnI GAANNNNTTC 1 cut(s) 165
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.