Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001931

Physical Location & Seq

Genomic Coordinates Forward (+)

10446959 .. 10454573

Gene Structure 7615 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0121100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGATCTCCGACGGAGTTGAAGACGAAGAGAAGTACCTGGCCGCCGGAATCGCCGGCCTTCAACAGAATGCCTTCTACATGCATCGCGCGCTGGACTCCAACAATCTCAGGGACGCTCTCAAGTACTCGGCTCAGATGCTATCGGAGCTCCGCACCTCCAAGCTTTCGCCTCACAAATACTACGAGCTCTATATGCGTGCGTTTGATGAATTGAGGAAACTGGAGATGTTCTTCAAGGAGGAGGCTCGGCGCGGTTGCTCTATTATTGATCTGTATGAGCTTGTGCAGCATGCTGGTAACATATTGCCCAGATTATATCTACTGTGTACAGTAGGATCTGTTTACATCAAATCTAAGGAAGCTCCTGCTAAGGATGTCCTGAAAGATCTTGTGGAAATGTCGCGTGGCATTCAGAATCCTGTACGTGGGCTCTTTTTAAGGAGTTACCTTTCTCAAGTCAGTAGGGATAAGTTGCCTGATATTGGGTCGGAGTATGAAGGGGATGCGGATACTGTCAGGGATGCTGTTGAGTTTGTTCTCCAAAACTTCACTGAGATGAACAAGCTTTGGGTGCGGATGCAACATCAGGGGCCTGCTAGGGAAAAGGAGAGACGGGAGAAAGAAAGGAGCGAGCTACGTGATCTTGTTGGTAAAAACCTGCATGTTCTCAGTCAGATAGAAGGTGTTGATCTGGATTTGTACAAAGAGACCGTGCTTCCCAGAGTCCTGGAGCAGGTTGTCAATTGTAAAGATGAGCTGGCACAGTTTTATTTGGTGGATTGCATTATTCAAGTCTTTCCTGATGAGTACCACTTGCAAACTCTTGATATATTGTTGGGTGCTTGCCCCCAACTTCAGCCATCTGTTGATATCAAGACAGTGCTATCCCAGTTAATGGAAAGGCTTTCCAACTATGCTGCCTCAAGCATAGAAGTGTTGCCCGAGTTCTTACAAGTAGAAGCTTTTTCTAAACTGAGCAATGCGATTGGAAAGGTGATTGAAGCGCACGTTGACATGCCTGTAATTGGTGTTGTTACTTTGTACTCATCCCTTCTTAAATTTACTCTCCATGTGCATCCGGATCGACTTGATTATGCTGATCAAGTGTTGGGGTCATTTGTTAAGAAGCTCTCCGGAAAAGGAAAAATTGAAGACAGCAGGGCAACAAAACAAGTTGTTGCACTTTTAAGTGCTCCATTGGAGAAATATAATGATATTGTGACTGCTTTGAAGCTTACAAATTATCCCCGTGTCATGGAATTCCTTGATGCTGGAACAAATAAAGTCATGGCAACTGTTATAATACAAAGCATTATGAAAAATACAACTCATATATTAACTGCTGAGAAGGTCGAGGCATTGTTTGAATTAATAAAAGGACTTATCGAGGATTTGGATGGGACTCTTCATGATGAGGTTGATGAAGATGATTTCAAGGAGGAGCAGAATTCTGTTGCGAGACTTATTCAAATGTTCCATAATGAAGATCCAGAAGAGATGTTTAAAATCATAGGTACTGTGCGGAAGCACATCATGTCTGGAGGACCAAAGCGCCTACCCTTTACTGTCCCTCCCCTAGTCTTTAATTCTCTAAAGTTGGTCAGGCAATTGCAAGGAGGACATGAAGATAAAGAAAATCCTTTTGGAGAAGAAGCGTCGACTACACCAAAGAAAATTTTTCAGCTCTTGTCTCAGACTATTGAGGCTCTGTTGAATGTGCCAGCTCCTGAGTTAGCTTTACGGCTGTACTTGCAATGTGCTGAGGCTGCAAATGACTGTGATTTAGAACCTGTTGCGTACGAATTCTTCACCCAAGCATATATTCTCTATGAAGAAGAAATTTCAGATTCGAAAGCACAGGTGACTGCAATTCATTTGATAATTGGGACTCTCCAGAGGATGCATGTATTTGGTGTTGAGAACAGAGATACTTTAACTCACAAAGCCACAGGGTATTCGGCAAAGCTTTTAAAGAAGCCTGATCAGTGCCGAGCTGTTTATGCTTGCTCGCATCTTTTCTGGGTTGACGATCAGGAGAACATGAAGGATGGGGAAAGGGTACTGATTTGCCTAAAGAGAGCTTTAAGGATTGCAAACGCTGCCCAGCAGATGGCCAACGCAACCCGTGGTAGCACCGGTTCAGCGACACTCTTCGTTGAAATACTGAACAAGTACCTTTATTTCTTTGAGAAGGGCAACCCACAGATAACTGTTGCTTCTGTACAAAGCCTGATTGAATTGATTACTACTGAAATGCAAAGCGACTCAACCCCAGCAGAGTCGGCTACAAATGCATTCTTTGCCAGCACGATGCGGTACATTCAATTCCAGAAGCAGAAAGGTGGCGCAGTTGGTGAAAAATATGAGCAAATCAAGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009909 GO:0009910 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0048519 GO:0048580 GO:0048581 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0060089 GO:0060860 GO:0060862 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:2000026 GO:2000241 GO:2000242

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

793

Amino Acids

89.73

Weight (kDa)

5.44

Isoelectric Point (pI)

44.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps35	PF03635	13 - 750	4.6e-289	Vacuolar protein sorting-associated protein 35

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001931)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g15270 FvH4_1g15350 FvH4_1g15430
rosa_chinensis	RchiOBHm_Chr2g0104571 RchiOBHm_Chr2g0104621 RchiOBHm_Chr2g0104631 RchiOBHm_Chr2g0104641
rosa_laevigata	RLG00000017191 RLG00000017336 RLG00000017337 RLG00000017338
rosa_multiflora	Rmu_sc0002316.1_g000034 Rmu_sc0002316.1_g000036 Rmu_sc0002316.1_g000045 Rmu_sc0002316.1_g000046
rosa_roxburghii	Rroxscaffold_2G00138880 Rroxscaffold_2G00138890 Rroxscaffold_2G00138930 Rroxscaffold_2G00138940
rosa_rugosa	Rorug02G0121000 Rorug02G0121100 Rorug02G0121200 Rorug02G0121300 Rorug02G0121300 Rorug02G0121400 Rorug02G0121500 Rorug02G0121600 Rorug02G0121600 Rorug02G0121700
rosa_samantha	Rh2AG171400 Rh2AG171700 Rh2AG171800 Rh2BG178700 Rh2BG178800 Rh2BG179100 Rh2BG179200 Rh2BG179300 Rh2CG176300 Rh2CG176600 Rh2DG177400 Rh2DG177500 Rh2DG178000 Rh2DG178100
rosa_wichuraiana	Rw2G013400 Rw2G013420 Rw2G013440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1301
Acc36I	ACCTGC	2 cut(s)	666, 724
AccB7I	CCANNNNNTGG	2 cut(s)	895, 2110
AccI	GTMKAC	1 cut(s)	1658
AccII	CGCG	4 cut(s)	87, 89, 252, 403
AccIII	TCCGGA	2 cut(s)	1078, 1133
AciI	CCGC	7 cut(s)	42, 151, 252, 506, 574, 1522, 2314
AclWI	GGATC	3 cut(s)	343, 1089, 1481
AcoI	YGGCCR	2 cut(s)	39, 2112
AcsI	RAATTY	6 cut(s)	1058, 1259, 1447, 1674, 1802, 1839
AcuI	CTGAAG	1 cut(s)	839
AfiI	CCNNNNNNNGG	7 cut(s)	425, 482, 733, 895, 1025, 1255, 2110
AgeI	ACCGGT	1 cut(s)	2135
AjnI	CCWGG	2 cut(s)	36, 726
AjuI	GAANNNNNNNTTGG	4 cut(s)	1626, 1658, 1806, 1838
Alw21I	GWGCWC	3 cut(s)	150, 189, 1195
Alw26I	GTCTC	4 cut(s)	604, 701, 1453, 1695
AlwI	GGATC	3 cut(s)	343, 1089, 1481
AlwNI	CAGNNNCTG	1 cut(s)	1727
Ama87I	CYCGRG	1 cut(s)	941
Aor13HI	TCCGGA	2 cut(s)	1078, 1133
AoxI	GGCC	4 cut(s)	39, 55, 590, 2112
ApeKI	GCWGC	4 cut(s)	286, 917, 1766, 2099
ApoI	RAATTY	6 cut(s)	1058, 1259, 1447, 1674, 1802, 1839
AseI	ATTAAT	1 cut(s)	1370
AsiGI	ACCGGT	1 cut(s)	2135
Asp700I	GAANNNNTTC	1 cut(s)	71
AspLEI	GCGC	6 cut(s)	89, 91, 252, 1006, 1554, 2348
AspS9I	GGNCC	2 cut(s)	590, 1544
AsuHPI	GGTGA	4 cut(s)	1006, 1801, 1873, 2366
AsuII	TTCGAA	1 cut(s)	1850
AvaI	CYCGRG	1 cut(s)	941
AvaII	GGWCC	1 cut(s)	1544
BalI	TGGCCA	1 cut(s)	2114
BanII	GRGCYC	3 cut(s)	150, 189, 432
BarI	GAAGNNNNNNTAC	2 cut(s)	1790, 1822
BbsI	GAAGAC	2 cut(s)	27, 1158
Bbv12I	GWGCWC	3 cut(s)	150, 189, 1195
BbvCI	CCTCAGC	1 cut(s)	1761
BbvI	GCAGC	4 cut(s)	298, 904, 1753, 2086
BccI	CCATC	4 cut(s)	868, 1391, 2042, 2104
BceAI	ACGGC	1 cut(s)	1757
BcgI	CGANNNNNNTGC	2 cut(s)	1064, 1098
BciT130I	CCWGG	2 cut(s)	38, 728
BciVI	GTATCC	1 cut(s)	502
BclI	TGATCA	2 cut(s)	1099, 1981
BcoDI	GTCTC	4 cut(s)	604, 701, 1453, 1695
BfaI	CTAG	2 cut(s)	597, 1577
BfoI	RGCGCY	1 cut(s)	1555
BfuAI	ACCTGC	2 cut(s)	666, 724
BfuI	GTATCC	1 cut(s)	502
BglII	AGATCT	1 cut(s)	385
BisI	GCNGC	5 cut(s)	42, 287, 918, 1767, 2100
BlsI	GCNGC	5 cut(s)	43, 288, 919, 1768, 2101
BmcAI	AGTACT	1 cut(s)	125
Bme1390I	CCNGG	2 cut(s)	38, 728
Bme18I	GGWCC	1 cut(s)	1544
BmeT110I	CYCGRG	1 cut(s)	941
BmgT120I	GGNCC	2 cut(s)	590, 1544
BmiI	GGNNCC	1 cut(s)	591
BmrFI	CCNGG	2 cut(s)	38, 728
BmrI	ACTGGG	1 cut(s)	883
BmuI	ACTGGG	1 cut(s)	883
BpiI	GAAGAC	2 cut(s)	27, 1158
BpmI	CTGGAG	4 cut(s)	242, 749, 1560, 1877
Bpu10I	CCTNAGC	2 cut(s)	369, 1761
Bpu14I	TTCGAA	1 cut(s)	1850
BpuEI	CTTGAG	3 cut(s)	104, 438, 907
BsaAI	YACGTR	2 cut(s)	425, 638
BsaI	GGTCTC	1 cut(s)	701
BsaJI	CCNNGG	1 cut(s)	2125
BsaWI	WCCGGW	3 cut(s)	1078, 1133, 2135
BsaXI	ACNNNNNCTCC	2 cut(s)	1555, 1585
Bsc4I	CCNNNNNNNGG	7 cut(s)	425, 482, 733, 895, 1025, 1255, 2110
Bse118I	RCCGGY	2 cut(s)	53, 2135
Bse1I	ACTGG	2 cut(s)	225, 889
Bse3DI	GCAATG	2 cut(s)	985, 1760
BseAI	TCCGGA	2 cut(s)	1078, 1133
BseBI	CCWGG	2 cut(s)	38, 728
BseDI	CCNNGG	1 cut(s)	2125
BseGI	GGATG	9 cut(s)	379, 508, 526, 582, 1046, 1075, 1402, 1905, 2053
BseLI	CCNNNNNNNGG	7 cut(s)	425, 482, 733, 895, 1025, 1255, 2110
BseMI	GCAATG	2 cut(s)	985, 1760
BseMII	CTCAG	9 cut(s)	121, 146, 543, 682, 965, 1335, 1706, 1719, 1752
BseNI	ACTGG	2 cut(s)	225, 889
BsePI	GCGCGC	1 cut(s)	87
BseRI	GAGGAG	2 cut(s)	254, 1454
BseXI	GCAGC	4 cut(s)	298, 904, 1753, 2086
BseYI	CCCAGC	2 cut(s)	2103, 2272
BsgI	GTGCAG	1 cut(s)	305
Bsh1236I	CGCG	4 cut(s)	87, 89, 252, 403
BshFI	GGCC	4 cut(s)	41, 57, 592, 2114
BshTI	ACCGGT	1 cut(s)	2135
BsiHKAI	GWGCWC	3 cut(s)	150, 189, 1195
BsiHKCI	CYCGRG	1 cut(s)	941
BsiSI	CCGG	5 cut(s)	45, 54, 1079, 1134, 2136
BsiWI	CGTACG	1 cut(s)	1797
BslFI	GGGAC	4 cut(s)	125, 1414, 1553, 1900
BslI	CCNNNNNNNGG	7 cut(s)	425, 482, 733, 895, 1025, 1255, 2110
BsmAI	GTCTC	4 cut(s)	604, 701, 1453, 1695
BsmBI	CGTCTC	1 cut(s)	604
BsmFI	GGGAC	4 cut(s)	125, 1414, 1553, 1900
BsmI	GAATGC	3 cut(s)	73, 408, 2294
BsnI	GGCC	4 cut(s)	41, 57, 592, 2114
Bso31I	GGTCTC	1 cut(s)	701
BsoBI	CYCGRG	1 cut(s)	941
Bsp119I	TTCGAA	1 cut(s)	1850
Bsp1286I	GDGCHC	4 cut(s)	150, 189, 432, 1195
Bsp13I	TCCGGA	2 cut(s)	1078, 1133
Bsp1407I	TGTACA	3 cut(s)	326, 699, 2221
BspACI	CCGC	7 cut(s)	42, 151, 252, 506, 574, 1522, 2314
BspANI	GGCC	4 cut(s)	41, 57, 592, 2114
BspCNI	CTCAG	9 cut(s)	120, 145, 544, 681, 966, 1336, 1705, 1720, 1753
BspEI	TCCGGA	2 cut(s)	1078, 1133
BspFNI	CGCG	4 cut(s)	87, 89, 252, 403
BspHI	TCATGA	1 cut(s)	1408
BspLI	GGNNCC	1 cut(s)	591
BspMI	ACCTGC	2 cut(s)	666, 724
BspPI	GGATC	3 cut(s)	343, 1089, 1481
BspT104I	TTCGAA	1 cut(s)	1850
BspTNI	GGTCTC	1 cut(s)	701
BsrDI	GCAATG	2 cut(s)	985, 1760
BsrFI	RCCGGY	2 cut(s)	53, 2135
BsrGI	TGTACA	3 cut(s)	326, 699, 2221
BsrI	ACTGG	2 cut(s)	225, 889
BssAI	RCCGGY	2 cut(s)	53, 2135
BssECI	CCNNGG	1 cut(s)	2125
BssHII	GCGCGC	1 cut(s)	87
Bst2UI	CCWGG	2 cut(s)	38, 728
Bst6I	CTCTTC	4 cut(s)	21, 1410, 1488, 2156
BstAPI	GCANNNNNTGC	2 cut(s)	2099, 2300
BstAUI	TGTACA	3 cut(s)	326, 699, 2221
BstBAI	YACGTR	2 cut(s)	425, 638
BstBI	TTCGAA	1 cut(s)	1850
BstDSI	CCRYGG	1 cut(s)	2125
BstENI	CCTNNNNNAGG	1 cut(s)	731
BstF5I	GGATG	9 cut(s)	379, 508, 526, 582, 1046, 1075, 1402, 1905, 2053
BstFNI	CGCG	4 cut(s)	87, 89, 252, 403
BstH2I	RGCGCY	1 cut(s)	1555
BstHHI	GCGC	6 cut(s)	89, 91, 252, 1006, 1554, 2348
BstMAI	GTCTC	4 cut(s)	604, 701, 1453, 1695
BstNI	CCWGG	2 cut(s)	38, 728
BstNSI	RCATGY	5 cut(s)	82, 293, 665, 1018, 1907
BstSCI	CCNGG	2 cut(s)	36, 726
BstUI	CGCG	4 cut(s)	87, 89, 252, 403
BstV1I	GCAGC	4 cut(s)	298, 904, 1753, 2086
BstV2I	GAAGAC	2 cut(s)	27, 1158
BstX2I	RGATCY	3 cut(s)	335, 385, 1486
BstXI	CCANNNNNNTGG	1 cut(s)	727
BstYI	RGATCY	3 cut(s)	335, 385, 1486
BsuI	GTATCC	1 cut(s)	502
BsuRI	GGCC	4 cut(s)	41, 57, 592, 2114
BtgI	CCRYGG	1 cut(s)	2125
BtgZI	GCGATG	1 cut(s)	68
BtsCI	GGATG	9 cut(s)	379, 508, 526, 582, 1046, 1075, 1402, 1905, 2053
BtsIMutI	CAGTG	3 cut(s)	549, 885, 1991
BveI	ACCTGC	2 cut(s)	666, 724
CaiI	CAGNNNCTG	1 cut(s)	1727
CciI	TCATGA	1 cut(s)	1408
CfoI	GCGC	6 cut(s)	89, 91, 252, 1006, 1554, 2348
Cfr10I	RCCGGY	2 cut(s)	53, 2135
Cfr13I	GGNCC	2 cut(s)	590, 1544
CseI	GACGC	2 cut(s)	122, 1644
CspAI	ACCGGT	1 cut(s)	2135
DraI	TTTAAA	2 cut(s)	1504, 1971
EaeI	YGGCCR	2 cut(s)	39, 2112
Eam1104I	CTCTTC	4 cut(s)	21, 1410, 1488, 2156
EarI	CTCTTC	4 cut(s)	21, 1410, 1488, 2156
Ecl136II	GAGCTC	2 cut(s)	148, 187
Eco24I	GRGCYC	3 cut(s)	150, 189, 432
Eco31I	GGTCTC	1 cut(s)	701
Eco32I	GATATC	1 cut(s)	871
Eco47I	GGWCC	1 cut(s)	1544
Eco53kI	GAGCTC	2 cut(s)	148, 187
Eco57I	CTGAAG	1 cut(s)	839
Eco88I	CYCGRG	1 cut(s)	941
EcoICRI	GAGCTC	2 cut(s)	148, 187
EcoNI	CCTNNNNNAGG	1 cut(s)	731
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRI	GAATTC	3 cut(s)	1259, 1447, 1802
EcoRII	CCWGG	2 cut(s)	36, 726
EcoRV	GATATC	1 cut(s)	871
EcoT22I	ATGCAT	3 cut(s)	84, 1905, 2296
EcoT38I	GRGCYC	3 cut(s)	150, 189, 432
Esp3I	CGTCTC	1 cut(s)	604
FaqI	GGGAC	4 cut(s)	125, 1414, 1553, 1900
FbaI	TGATCA	2 cut(s)	1099, 1981
FblI	GTMKAC	1 cut(s)	1658
Fnu4HI	GCNGC	5 cut(s)	42, 287, 918, 1767, 2100
FokI	GGATG	9 cut(s)	386, 515, 533, 589, 1033, 1062, 1409, 1912, 2060
FriOI	GRGCYC	3 cut(s)	150, 189, 432
Fsp4HI	GCNGC	5 cut(s)	42, 287, 918, 1767, 2100
FspBI	CTAG	2 cut(s)	597, 1577
GlaI	GCGC	6 cut(s)	88, 90, 251, 1005, 1553, 2347
GluI	GCNGC	5 cut(s)	42, 287, 918, 1767, 2100
GsaI	CCCAGC	2 cut(s)	2107, 2276
GsuI	CTGGAG	4 cut(s)	242, 749, 1560, 1877
HaeII	RGCGCY	1 cut(s)	1555
HaeIII	GGCC	4 cut(s)	41, 57, 592, 2114
HapII	CCGG	5 cut(s)	45, 54, 1079, 1134, 2136
HgaI	GACGC	2 cut(s)	122, 1644
HhaI	GCGC	6 cut(s)	89, 91, 252, 1006, 1554, 2348
Hin6I	GCGC	6 cut(s)	87, 89, 250, 1004, 1552, 2346
HinP1I	GCGC	6 cut(s)	87, 89, 250, 1004, 1552, 2346
HincII	GTYRAC	3 cut(s)	1012, 1659, 2026
HindII	GTYRAC	3 cut(s)	1012, 1659, 2026
HindIII	AAGCTT	5 cut(s)	161, 563, 960, 1232, 1964
HinfI	GANTC	9 cut(s)	48, 95, 415, 723, 1402, 1847, 1888, 2264, 2279
HpaII	CCGG	5 cut(s)	45, 54, 1079, 1134, 2136
HphI	GGTGA	4 cut(s)	1006, 1801, 1873, 2366
Hpy166II	GTNNAC	5 cut(s)	327, 343, 1012, 1659, 2026
Hpy188I	TCNGA	8 cut(s)	10, 135, 145, 414, 490, 675, 1695, 1846
Hpy8I	GTNNAC	5 cut(s)	327, 343, 1012, 1659, 2026
Hpy99I	CGWCG	2 cut(s)	14, 1660
HpyAV	CCTTC	8 cut(s)	68, 82, 491, 674, 1061, 1342, 2038, 2185
HpyCH4IV	ACGT	3 cut(s)	424, 637, 1008
HpySE526I	ACGT	3 cut(s)	424, 637, 1008
HspAI	GCGC	6 cut(s)	87, 89, 250, 1004, 1552, 2346
Kpn2I	TCCGGA	2 cut(s)	1078, 1133
KroI	GCCGGC	1 cut(s)	53
KroNI	GCCGGC	1 cut(s)	55
Ksp22I	TGATCA	2 cut(s)	1099, 1981
LmnI	GCTCC	8 cut(s)	145, 153, 367, 627, 730, 1198, 1441, 1729
Lsp1109I	GCAGC	4 cut(s)	298, 904, 1753, 2086
MaeI	CTAG	2 cut(s)	597, 1577
MaeII	ACGT	3 cut(s)	424, 637, 1008
MaeIII	GTNAC	5 cut(s)	296, 443, 1033, 1219, 1861
MfeI	CAATTG	2 cut(s)	742, 1607
MflI	RGATCY	3 cut(s)	335, 385, 1486
MhlI	GDGCHC	4 cut(s)	150, 189, 432, 1195
MlsI	TGGCCA	1 cut(s)	2114
MluNI	TGGCCA	1 cut(s)	2114
MlyI	GAGTC	6 cut(s)	89, 732, 1396, 1882, 2258, 2288
MmeI	TCCRAC	4 cut(s)	33, 123, 468, 933
Mox20I	TGGCCA	1 cut(s)	2114
Mph1103I	ATGCAT	3 cut(s)	84, 1905, 2296
MroI	TCCGGA	2 cut(s)	1078, 1133
MroNI	GCCGGC	1 cut(s)	53
MroXI	GAANNNNTTC	1 cut(s)	71
MscI	TGGCCA	1 cut(s)	2114
MslI	CAYNNNNRTG	2 cut(s)	554, 932
Msp20I	TGGCCA	1 cut(s)	2114
MspI	CCGG	5 cut(s)	45, 54, 1079, 1134, 2136
MspR9I	CCNGG	2 cut(s)	38, 728
MunI	CAATTG	2 cut(s)	742, 1607
Mva1269I	GAATGC	3 cut(s)	73, 408, 2294
MvaI	CCWGG	2 cut(s)	38, 728
MvnI	CGCG	4 cut(s)	87, 89, 252, 403
NaeI	GCCGGC	1 cut(s)	55
NgoMIV	GCCGGC	1 cut(s)	53
NlaIV	GGNNCC	1 cut(s)	591
NmeAIII	GCCGAG	3 cut(s)	107, 226, 2015
NmuCI	GTSAC	2 cut(s)	1219, 1861
NsiI	ATGCAT	3 cut(s)	84, 1905, 2296
NspI	RCATGY	5 cut(s)	82, 293, 665, 1018, 1907
NspV	TTCGAA	1 cut(s)	1850
PaeI	GCATGC	1 cut(s)	293
PagI	TCATGA	1 cut(s)	1408
PauI	GCGCGC	1 cut(s)	87
PctI	GAATGC	3 cut(s)	73, 408, 2294
PdiI	GCCGGC	1 cut(s)	55
PdmI	GAANNNNTTC	1 cut(s)	71
PfeI	GAWTC	3 cut(s)	48, 415, 1847
Pfl23II	CGTACG	1 cut(s)	1797
PflMI	CCANNNNNTGG	2 cut(s)	895, 2110
PfoI	TCCNGGA	1 cut(s)	726
PinAI	ACCGGT	1 cut(s)	2135
PkrI	GCNGC	5 cut(s)	43, 288, 919, 1768, 2101
PleI	GAGTC	6 cut(s)	89, 731, 1396, 1882, 2258, 2287
PpsI	GAGTC	6 cut(s)	89, 731, 1396, 1882, 2258, 2287
Ppu21I	YACGTR	2 cut(s)	425, 638
PshBI	ATTAAT	1 cut(s)	1370
PsiI	TTATAA	1 cut(s)	1301
Psp124BI	GAGCTC	2 cut(s)	150, 189
Psp6I	CCWGG	2 cut(s)	36, 726
PspFI	CCCAGC	2 cut(s)	2103, 2272
PspGI	CCWGG	2 cut(s)	36, 726
PspLI	CGTACG	1 cut(s)	1797
PspN4I	GGNNCC	1 cut(s)	591
PspPI	GGNCC	2 cut(s)	590, 1544
PsrI	GAACNNNNNNTAC	2 cut(s)	2122, 2154
PstNI	CAGNNNCTG	1 cut(s)	1727
PsuI	RGATCY	3 cut(s)	335, 385, 1486
PteI	GCGCGC	1 cut(s)	87
RseI	CAYNNNNRTG	2 cut(s)	554, 932
SacI	GAGCTC	2 cut(s)	150, 189
SalI	GTCGAC	1 cut(s)	1657
SatI	GCNGC	5 cut(s)	42, 287, 918, 1767, 2100
Sau96I	GGNCC	2 cut(s)	590, 1544
ScaI	AGTACT	1 cut(s)	125
SchI	GAGTC	6 cut(s)	89, 732, 1396, 1882, 2258, 2288
ScrFI	CCNGG	2 cut(s)	38, 728
SduI	GDGCHC	4 cut(s)	150, 189, 432, 1195
SfuI	TTCGAA	1 cut(s)	1850
SinI	GGWCC	1 cut(s)	1544
SmiMI	CAYNNNNRTG	2 cut(s)	554, 932
SmlI	CTYRAG	3 cut(s)	119, 453, 922
SmoI	CTYRAG	3 cut(s)	119, 453, 922
SphI	GCATGC	1 cut(s)	293
SsiI	CCGC	7 cut(s)	42, 151, 252, 506, 574, 1522, 2314
SspMI	CTAG	2 cut(s)	597, 1577
SstI	GAGCTC	2 cut(s)	150, 189
StyD4I	CCNGG	2 cut(s)	36, 726
TaiI	ACGT	3 cut(s)	427, 640, 1011
TaqI	TCGA	5 cut(s)	1084, 1353, 1386, 1658, 1850
TatI	WGTACW	6 cut(s)	123, 326, 699, 1041, 1746, 2221
TauI	GCSGC	1 cut(s)	44
TfiI	GAWTC	3 cut(s)	48, 415, 1847
TscAI	CASTG	3 cut(s)	556, 885, 1991
TseFI	GTSAC	2 cut(s)	1219, 1861
TseI	GCWGC	4 cut(s)	286, 917, 1766, 2099
Tsp45I	GTSAC	2 cut(s)	1219, 1861
TspGWI	ACGGA	1 cut(s)	27
TspRI	CASTG	3 cut(s)	556, 885, 1991
Van91I	CCANNNNNTGG	2 cut(s)	895, 2110
VpaK11BI	GGWCC	1 cut(s)	1544
VspI	ATTAAT	1 cut(s)	1370
XagI	CCTNNNNNAGG	1 cut(s)	731
XapI	RAATTY	6 cut(s)	1058, 1259, 1447, 1674, 1802, 1839
XceI	RCATGY	5 cut(s)	82, 293, 665, 1018, 1907
XmiI	GTMKAC	1 cut(s)	1658
XmnI	GAANNNNTTC	1 cut(s)	71
XspI	CTAG	2 cut(s)	597, 1577
ZrmI	AGTACT	1 cut(s)	125
Zsp2I	ATGCAT	3 cut(s)	84, 1905, 2296

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.