Rorug06G0229800

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002048
Physical Location & Seq
Reverse (-)
36885116 .. 36889687
4572 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0229800.1

Sequence Viewer

Length: 1776 bp
ATGGAAGTAGTAGAAGATGTTGTGATTGTGGGAGCTGGAATTTCTGGCCTTACAACCTCCTTGGGACTTCACAGGCTGGGCATTAGAAGCTTAGTGCTGGAATCATTTGATAGTTTGAGGATAACAGGGTTTGGATTGTCATTATGGACAAATGCATGGAGGGCTTTAGATGCCATTGGTATTGGCGATTGTCTTCGGCCGCAACATGTGACTCTTCTCGGGAGTGTGCTTTTCTCAAGAATTTCGGGGCTTCAGATGTCTGAGAGGTCATACAGGGACAAAGGAAAACAAGGAGACCAAGAAGTTCGATGTGTGAAAAGGAATTTGTTGTTGGAAGCCCTTGCAAGTGAACTCCCCAGCGGTACCATTAGGTTCTCATCGAAGGTTGTTTCCATTGAGGAATCAGGGTACCTTAAGCTTGTGCACCTTGCAGATGGAACCATCCTCAAAGCCAAGGTTTTGGTTGGGTGTGATGGAGTGAACTCAGTGGTGGCAAAATGGCTGGGCTTCAAGAAGCCAGTCTTTTCAGAGAGATCCGTGGTTAGAGGTTGTGCTAACTTCAAGAGCAGTCATGGTTTTGAACCCATTACCATGCAGCACTTTGGCAATGGTGTTAGATCTGGTGCTGCTCCTTGTGATGATAAAAGTGTTTATTGGTTCTTTGGCTGGTCTCCCTCCAGCCAAGAGAAAGAGCTAGAGAAGAACCCTGCTCAGTTGAAAAAATATATCTTACCCAAGCTCGGAAAGGTACAAGATGAAATAAGGGCTGTTATAGAAAACACTGAATTGGATGCTTTTGTATCCTCTCCAGTGAGATATAGGCACCCTTGGGAACTGCTTTGGGGAAATATTAGCAAAGGTGGTGTATGTGTTGCTGGAGACGCACTCCACCCCATGACCCCAGACATCGGCCAAGGTGGTTGTGCTGCATTAGAAGACGGTGTTGTATTAGCAAGATGTCTCGGTGAGGCTTTGTTGAAGAACTGGAGGCAAGAAATTAGAGATAAAGATGAAGAACGAAAAGAGGAATGCTGGGGATTTGAAGTAGTGTTGGAATCATGGGATAGTTTGAGGACTACAGGGTTTGTTTTGAATACACGGACAAATGCCTGGAAAGCCTTAGAAGCCATTGGTGTTGCTGATGCTCTTCGGCTAATAAATTACAGATTTCGTGGAGGAGACCATGAAGCTCGTTGTGTGCAAAGGAGGCTGTTGTTGGAATCCCTTGCAAATGAACTTCCTAGTGGCACAACCAGGTTCTCATCAAAAGTTGTTTCAATCGAAGAATCAGGCTACTTTAAGCTTCTGCATCTTGCTGATGGAACCATCCTTAAAGTCTTGGTTGGGTGTGATGGAGTCAACTCTGTGGTTGGAAAATGGCTAAACTTCAAGCAGCTATCTTTTACAGGAAAATCCGCTATTAGAGGTCAGGCAAACTTCAAGAGCAATCATGGGTTTGATCCCAAATCCATGCGGTTCTTCGAGCATGGTGTTAGATCTGGTGTCATCCCTTGTGATAAAAAAAAAGATGTTTATCGATCCTTAAATTTGTTGTTTACTGCTCATGTCTTAGAGAAAGGTTTGGAAGAGAATCCAGCTCAATTGAGGCAATTTGTGTTGAGCAAGCTAGGAAAGATTCCTGATGCAGTAAGGGCTACTATAGAAAACACTGAACTGGATGCTTTTATATTGTCTCCCTTGAGATATAAACCTCCTTGGGAGGTTCTTTGGGGACAGCTAATGTGGTTGGTTTTGTTGGAAAGTGAGCTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

591

Amino Acids

65.66

Weight (kDa)

8.58

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_3 PF01494 6 - 326 5.1e-23 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 362, 408
AccB1I GGYRCC 3 cut(s) 362, 408, 822
AciI CCGC 4 cut(s) 200, 360, 1416, 1474
AclWI GGATC 3 cut(s) 528, 1454, 1533
AcoI YGGCCR 2 cut(s) 197, 910
AcsI RAATTY 4 cut(s) 39, 240, 322, 1546
AcuI CTGAAG 1 cut(s) 236
AfaI GTAC 3 cut(s) 364, 410, 750
AfiI CCNNNNNNNGG 2 cut(s) 740, 908
AflII CTTAAG 1 cut(s) 413
AflIII ACRYGT 1 cut(s) 205
AjnI CCWGG 2 cut(s) 1109, 1253
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 6 cut(s) 288, 675, 873, 965, 1173, 1698
Alw44I GTGCAC 1 cut(s) 422
AlwI GGATC 3 cut(s) 528, 1454, 1533
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 3 cut(s) 46, 197, 910
ApaLI GTGCAC 1 cut(s) 422
ApeKI GCWGC 4 cut(s) 595, 626, 926, 1393
ApoI RAATTY 4 cut(s) 39, 240, 322, 1546
Asp718I GGTACC 2 cut(s) 362, 408
AsuHPI GGTGA 1 cut(s) 977
AvaI CYCGRG 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 426
BanI GGYRCC 3 cut(s) 362, 408, 822
BbsI GAAGAC 2 cut(s) 185, 942
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 4 cut(s) 607, 613, 913, 1405
BccI CCATC 6 cut(s) 428, 449, 467, 1313, 1334, 1346
BciT130I CCWGG 2 cut(s) 1111, 1255
BciVI GTATCC 1 cut(s) 811
BcoDI GTCTC 6 cut(s) 288, 675, 873, 965, 1173, 1698
BfaI CTAG 3 cut(s) 695, 1242, 1628
BfmI CTRYAG 2 cut(s) 1077, 1659
BfrI CTTAAG 1 cut(s) 413
BfuI GTATCC 1 cut(s) 811
BglII AGATCT 2 cut(s) 617, 1496
BisI GCNGC 5 cut(s) 200, 596, 627, 927, 1394
BlsI GCNGC 5 cut(s) 201, 597, 628, 928, 1395
Bme1390I CCNGG 2 cut(s) 1111, 1255
BmeT110I CYCGRG 1 cut(s) 218
BmiI GGNNCC 5 cut(s) 364, 410, 439, 824, 1324
BmrFI CCNGG 2 cut(s) 1111, 1255
BmsI GCATC 6 cut(s) 160, 781, 1132, 1318, 1633, 1669
BpiI GAAGAC 2 cut(s) 185, 942
BplI GAGNNNNNCTC 2 cut(s) 870, 902
BpmI CTGGAG 4 cut(s) 661, 792, 897, 1006
BpuEI CTTGAG 2 cut(s) 220, 1720
Bsa29I ATCGAT 1 cut(s) 1537
BsaBI GATNNNNATC 1 cut(s) 1533
BsaI GGTCTC 3 cut(s) 288, 675, 1173
BsaJI CCNNGG 6 cut(s) 60, 453, 537, 827, 913, 1715
Bsc4I CCNNNNNNNGG 2 cut(s) 740, 908
Bse1I ACTGG 4 cut(s) 518, 809, 989, 1680
Bse3DI GCAATG 1 cut(s) 613
Bse8I GATNNNNATC 1 cut(s) 1533
BseBI CCWGG 2 cut(s) 1111, 1255
BseCI ATCGAT 1 cut(s) 1537
BseDI CCNNGG 6 cut(s) 60, 453, 537, 827, 913, 1715
BseGI GGATG 5 cut(s) 441, 796, 1326, 1506, 1684
BseJI GATNNNNATC 1 cut(s) 1533
BseLI CCNNNNNNNGG 2 cut(s) 740, 908
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 3 cut(s) 252, 498, 725
BseNI ACTGG 4 cut(s) 518, 809, 989, 1680
BseRI GAGGAG 1 cut(s) 1191
BseSI GKGCMC 1 cut(s) 426
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 4 cut(s) 607, 613, 913, 1405
BseYI CCCAGC 4 cut(s) 76, 356, 502, 1032
Bsh1285I CGRYCG 1 cut(s) 200
BshFI GGCC 3 cut(s) 48, 199, 912
BshNI GGYRCC 3 cut(s) 362, 408, 822
BshVI ATCGAT 1 cut(s) 1537
BsiEI CGRYCG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 1 cut(s) 218
BslFI GGGAC 3 cut(s) 78, 290, 1746
BslI CCNNNNNNNGG 2 cut(s) 740, 908
BsmAI GTCTC 6 cut(s) 288, 675, 873, 965, 1173, 1698
BsmBI CGTCTC 1 cut(s) 873
BsmFI GGGAC 3 cut(s) 78, 290, 1746
BsmI GAATGC 1 cut(s) 1034
BsnI GGCC 3 cut(s) 48, 199, 912
Bso31I GGTCTC 3 cut(s) 288, 675, 1173
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 5 cut(s) 533, 617, 1459, 1496, 1538
BspACI CCGC 4 cut(s) 200, 360, 1416, 1474
BspANI GGCC 3 cut(s) 48, 199, 912
BspCNI CTCAG 3 cut(s) 253, 497, 724
BspDI ATCGAT 1 cut(s) 1537
BspLI GGNNCC 5 cut(s) 364, 410, 439, 824, 1324
BspPI GGATC 3 cut(s) 528, 1454, 1533
BspQI GCTCTTC 1 cut(s) 1152
BspT107I GGYRCC 3 cut(s) 362, 408, 822
BspTI CTTAAG 1 cut(s) 413
BspTNI GGTCTC 3 cut(s) 288, 675, 1173
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 4 cut(s) 518, 809, 989, 1680
BssECI CCNNGG 6 cut(s) 60, 453, 537, 827, 913, 1715
BssMI GATC 5 cut(s) 533, 617, 1459, 1496, 1538
BssT1I CCWWGG 5 cut(s) 60, 453, 827, 913, 1715
Bst2UI CCWGG 2 cut(s) 1111, 1255
Bst4CI ACNGT 1 cut(s) 941
Bst6I CTCTTC 3 cut(s) 219, 1152, 1581
BstAFI CTTAAG 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 1625
BstDEI CTNAG 6 cut(s) 91, 261, 484, 711, 1120, 1570
BstDSI CCRYGG 1 cut(s) 537
BstF5I GGATG 5 cut(s) 441, 796, 1326, 1506, 1684
BstKTI GATC 5 cut(s) 536, 620, 1462, 1499, 1541
BstMAI GTCTC 6 cut(s) 288, 675, 873, 965, 1173, 1698
BstMBI GATC 5 cut(s) 533, 617, 1459, 1496, 1538
BstMCI CGRYCG 1 cut(s) 200
BstMWI GCNNNNNNNGC 8 cut(s) 87, 161, 170, 881, 1115, 1124, 1207, 1652
BstNI CCWGG 2 cut(s) 1111, 1255
BstNSI RCATGY 1 cut(s) 209
BstSCI CCNGG 2 cut(s) 1109, 1253
BstSFI CTRYAG 2 cut(s) 1077, 1659
BstSLI GKGCMC 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 607, 613, 913, 1405
BstV2I GAAGAC 2 cut(s) 185, 942
BstX2I RGATCY 3 cut(s) 533, 617, 1496
BstXI CCANNNNNNTGG 1 cut(s) 460
BstYI RGATCY 3 cut(s) 533, 617, 1496
BstZI CGGCCG 1 cut(s) 197
Bsu15I ATCGAT 1 cut(s) 1537
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 3 cut(s) 48, 199, 912
BsuTUI ATCGAT 1 cut(s) 1537
BtgI CCRYGG 1 cut(s) 537
BtsCI GGATG 5 cut(s) 441, 796, 1326, 1506, 1684
BtsIMutI CAGTG 4 cut(s) 492, 780, 816, 1668
Cac8I GCNNGC 1 cut(s) 1625
ClaI ATCGAT 1 cut(s) 1537
CseI GACGC 1 cut(s) 890
CsiI ACCWGGT 1 cut(s) 1253
Csp6I GTAC 3 cut(s) 363, 409, 749
CviQI GTAC 3 cut(s) 363, 409, 749
DdeI CTNAG 6 cut(s) 91, 261, 484, 711, 1120, 1570
DpnI GATC 5 cut(s) 535, 619, 1461, 1498, 1540
DpnII GATC 5 cut(s) 533, 617, 1459, 1496, 1538
EaeI YGGCCR 2 cut(s) 197, 910
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 3 cut(s) 219, 1152, 1581
EarI CTCTTC 3 cut(s) 219, 1152, 1581
EclXI CGGCCG 1 cut(s) 197
Eco130I CCWWGG 5 cut(s) 60, 453, 827, 913, 1715
Eco31I GGTCTC 3 cut(s) 288, 675, 1173
Eco52I CGGCCG 1 cut(s) 197
Eco57I CTGAAG 1 cut(s) 236
Eco88I CYCGRG 1 cut(s) 218
EcoRII CCWGG 2 cut(s) 1109, 1253
EcoT14I CCWWGG 5 cut(s) 60, 453, 827, 913, 1715
EcoT22I ATGCAT 1 cut(s) 157
ErhI CCWWGG 5 cut(s) 60, 453, 827, 913, 1715
Esp3I CGTCTC 1 cut(s) 873
FalI AAGNNNNNCTT 2 cut(s) 506, 538
FaqI GGGAC 3 cut(s) 78, 290, 1746
Fnu4HI GCNGC 5 cut(s) 200, 596, 627, 927, 1394
FokI GGATG 5 cut(s) 428, 803, 1313, 1493, 1691
Fsp4HI GCNGC 5 cut(s) 200, 596, 627, 927, 1394
FspBI CTAG 3 cut(s) 695, 1242, 1628
GluI GCNGC 5 cut(s) 200, 596, 627, 927, 1394
GsaI CCCAGC 4 cut(s) 80, 360, 506, 1036
GsuI CTGGAG 4 cut(s) 661, 792, 897, 1006
HaeIII GGCC 3 cut(s) 48, 199, 912
HgaI GACGC 1 cut(s) 890
HincII GTYRAC 1 cut(s) 1360
HindII GTYRAC 1 cut(s) 1360
HindIII AAGCTT 3 cut(s) 88, 416, 1301
HinfI GANTC 9 cut(s) 101, 211, 401, 1055, 1220, 1286, 1356, 1591, 1636
HphI GGTGA 1 cut(s) 977
Hpy166II GTNNAC 5 cut(s) 350, 424, 481, 1360, 1557
Hpy188I TCNGA 4 cut(s) 255, 262, 529, 743
Hpy188III TCNNGA 6 cut(s) 220, 237, 511, 562, 1441, 1640
Hpy8I GTNNAC 5 cut(s) 350, 424, 481, 1360, 1557
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 1 cut(s) 941
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 161, 170, 881, 1115, 1124, 1207, 1652
HpyF3I CTNAG 6 cut(s) 91, 261, 484, 711, 1120, 1570
KpnI GGTACC 2 cut(s) 366, 412
Kzo9I GATC 5 cut(s) 533, 617, 1459, 1496, 1538
LguI GCTCTTC 1 cut(s) 1152
LmnI GCTCC 2 cut(s) 32, 634
Lsp1109I GCAGC 4 cut(s) 607, 613, 913, 1405
LweI GCATC 6 cut(s) 160, 781, 1132, 1318, 1633, 1669
MabI ACCWGGT 1 cut(s) 1253
MaeI CTAG 3 cut(s) 695, 1242, 1628
MaeIII GTNAC 1 cut(s) 208
MalI GATC 5 cut(s) 535, 619, 1461, 1498, 1540
MboI GATC 5 cut(s) 533, 617, 1459, 1496, 1538
MfeI CAATTG 1 cut(s) 1601
MflI RGATCY 3 cut(s) 533, 617, 1496
MhlI GDGCHC 1 cut(s) 426
MlyI GAGTC 2 cut(s) 205, 1365
MmeI TCCRAC 5 cut(s) 312, 1032, 1197, 1351, 1737
Mph1103I ATGCAT 1 cut(s) 157
MseI TTAA 4 cut(s) 414, 1299, 1332, 1544
MslI CAYNNNNRTG 1 cut(s) 590
MspA1I CMGCKG 1 cut(s) 360
MspCI CTTAAG 1 cut(s) 413
MspR9I CCNGG 2 cut(s) 1111, 1255
MunI CAATTG 1 cut(s) 1601
Mva1269I GAATGC 1 cut(s) 1034
MvaI CCWGG 2 cut(s) 1111, 1255
MwoI GCNNNNNNNGC 8 cut(s) 87, 161, 170, 881, 1115, 1124, 1207, 1652
NdeII GATC 5 cut(s) 533, 617, 1459, 1496, 1538
NlaIV GGNNCC 5 cut(s) 364, 410, 439, 824, 1324
NmuCI GTSAC 1 cut(s) 208
NsiI ATGCAT 1 cut(s) 157
NspI RCATGY 1 cut(s) 209
PciI ACATGT 1 cut(s) 205
PciSI GCTCTTC 1 cut(s) 1152
PctI GAATGC 1 cut(s) 1034
PfeI GAWTC 7 cut(s) 101, 401, 1055, 1220, 1286, 1591, 1636
PkrI GCNGC 5 cut(s) 201, 597, 628, 928, 1395
PleI GAGTC 2 cut(s) 205, 1364
PpsI GAGTC 2 cut(s) 205, 1364
PscI ACATGT 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 1109, 1253
PspFI CCCAGC 4 cut(s) 76, 356, 502, 1032
PspGI CCWGG 2 cut(s) 1109, 1253
PspN4I GGNNCC 5 cut(s) 364, 410, 439, 824, 1324
PsuI RGATCY 3 cut(s) 533, 617, 1496
RsaI GTAC 3 cut(s) 364, 410, 750
RsaNI GTAC 3 cut(s) 363, 409, 749
RseI CAYNNNNRTG 1 cut(s) 590
SapI GCTCTTC 1 cut(s) 1152
SaqAI TTAA 4 cut(s) 414, 1299, 1332, 1544
SatI GCNGC 5 cut(s) 200, 596, 627, 927, 1394
Sau3AI GATC 5 cut(s) 533, 617, 1459, 1496, 1538
SchI GAGTC 2 cut(s) 205, 1365
ScrFI CCNGG 2 cut(s) 1111, 1255
SduI GDGCHC 1 cut(s) 426
SexAI ACCWGGT 1 cut(s) 1253
SfaNI GCATC 6 cut(s) 160, 781, 1132, 1318, 1633, 1669
SfcI CTRYAG 2 cut(s) 1077, 1659
SmiMI CAYNNNNRTG 1 cut(s) 590
SmlI CTYRAG 3 cut(s) 235, 413, 1699
SmoI CTYRAG 3 cut(s) 235, 413, 1699
SsiI CCGC 4 cut(s) 200, 360, 1416, 1474
SspI AATATT 1 cut(s) 850
SspMI CTAG 3 cut(s) 695, 1242, 1628
StyD4I CCNGG 2 cut(s) 1109, 1253
StyI CCWWGG 5 cut(s) 60, 453, 827, 913, 1715
TaaI ACNGT 1 cut(s) 941
TaqI TCGA 5 cut(s) 307, 380, 1281, 1482, 1537
TauI GCSGC 1 cut(s) 202
TfiI GAWTC 7 cut(s) 101, 401, 1055, 1220, 1286, 1591, 1636
Tru1I TTAA 4 cut(s) 414, 1299, 1332, 1544
Tru9I TTAA 4 cut(s) 414, 1299, 1332, 1544
TscAI CASTG 4 cut(s) 492, 787, 816, 1675
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 4 cut(s) 595, 626, 926, 1393
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 4 cut(s) 771, 1026, 1200, 1248
TspGWI ACGGA 2 cut(s) 526, 1114
TspRI CASTG 4 cut(s) 492, 787, 816, 1675
Vha464I CTTAAG 1 cut(s) 413
VneI GTGCAC 1 cut(s) 422
XapI RAATTY 4 cut(s) 39, 240, 322, 1546
XceI RCATGY 1 cut(s) 209
XspI CTAG 3 cut(s) 695, 1242, 1628
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.