abundant protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 29, 64, 248 |
| AcuI | CTGAAG | 1 cut(s) | 150 |
| AfaI | GTAC | 1 cut(s) | 281 |
| AgsI | TTSAA | 1 cut(s) | 110 |
| AjnI | CCWGG | 1 cut(s) | 82 |
| AluBI | AGCT | 2 cut(s) | 46, 113 |
| AluI | AGCT | 2 cut(s) | 46, 113 |
| Alw21I | GWGCWC | 1 cut(s) | 218 |
| Alw26I | GTCTC | 2 cut(s) | 65, 146 |
| ApeKI | GCWGC | 1 cut(s) | 245 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 258 |
| Bbv12I | GWGCWC | 1 cut(s) | 218 |
| BbvI | GCAGC | 1 cut(s) | 232 |
| BccI | CCATC | 1 cut(s) | 187 |
| BciT130I | CCWGG | 1 cut(s) | 84 |
| BcoDI | GTCTC | 2 cut(s) | 65, 146 |
| BisI | GCNGC | 1 cut(s) | 246 |
| BlsI | GCNGC | 1 cut(s) | 247 |
| Bme1390I | CCNGG | 1 cut(s) | 84 |
| BmrFI | CCNGG | 1 cut(s) | 84 |
| BmsI | GCATC | 1 cut(s) | 14 |
| BpuEI | CTTGAG | 1 cut(s) | 99 |
| BsaWI | WCCGGW | 1 cut(s) | 230 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 60, 90 |
| Bse1I | ACTGG | 1 cut(s) | 97 |
| BseBI | CCWGG | 1 cut(s) | 84 |
| BseMII | CTCAG | 1 cut(s) | 203 |
| BseNI | ACTGG | 1 cut(s) | 97 |
| BseRI | GAGGAG | 1 cut(s) | 172 |
| BseXI | GCAGC | 1 cut(s) | 232 |
| BsiHKAI | GWGCWC | 1 cut(s) | 218 |
| BsiSI | CCGG | 1 cut(s) | 231 |
| BsmAI | GTCTC | 2 cut(s) | 65, 146 |
| BsmBI | CGTCTC | 1 cut(s) | 146 |
| BsmI | GAATGC | 1 cut(s) | 258 |
| Bsp1286I | GDGCHC | 1 cut(s) | 218 |
| BspACI | CCGC | 3 cut(s) | 29, 64, 248 |
| BspCNI | CTCAG | 1 cut(s) | 204 |
| BspHI | TCATGA | 2 cut(s) | 175, 187 |
| BsrI | ACTGG | 1 cut(s) | 97 |
| Bst2UI | CCWGG | 1 cut(s) | 84 |
| Bst4CI | ACNGT | 1 cut(s) | 76 |
| BstC8I | GCNNGC | 1 cut(s) | 243 |
| BstDEI | CTNAG | 2 cut(s) | 212, 288 |
| BstMAI | GTCTC | 2 cut(s) | 65, 146 |
| BstNI | CCWGG | 1 cut(s) | 84 |
| BstSCI | CCNGG | 1 cut(s) | 82 |
| BstV1I | GCAGC | 1 cut(s) | 232 |
| Cac8I | GCNNGC | 1 cut(s) | 243 |
| CciI | TCATGA | 2 cut(s) | 175, 187 |
| Csp6I | GTAC | 1 cut(s) | 280 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 187, 222 |
| CviAII | CATG | 2 cut(s) | 176, 188 |
| CviJI | RGCY | 2 cut(s) | 46, 113 |
| CviKI_1 | RGCY | 2 cut(s) | 46, 113 |
| CviQI | GTAC | 1 cut(s) | 280 |
| DdeI | CTNAG | 2 cut(s) | 212, 288 |
| Eco57I | CTGAAG | 1 cut(s) | 150 |
| EcoRII | CCWGG | 1 cut(s) | 82 |
| Esp3I | CGTCTC | 1 cut(s) | 146 |
| FaeI | CATG | 2 cut(s) | 179, 191 |
| FaiI | YATR | 3 cut(s) | 177, 189, 221 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 108, 140 |
| FatI | CATG | 2 cut(s) | 175, 187 |
| FauI | CCCGC | 1 cut(s) | 57 |
| Fnu4HI | GCNGC | 1 cut(s) | 246 |
| Fsp4HI | GCNGC | 1 cut(s) | 246 |
| GluI | GCNGC | 1 cut(s) | 246 |
| HapII | CCGG | 1 cut(s) | 231 |
| Hin1II | CATG | 2 cut(s) | 179, 191 |
| HinfI | GANTC | 4 cut(s) | 25, 103, 272, 292 |
| HpaII | CCGG | 1 cut(s) | 231 |
| Hpy188I | TCNGA | 2 cut(s) | 10, 213 |
| Hpy188III | TCNNGA | 4 cut(s) | 107, 116, 176, 188 |
| HpyAV | CCTTC | 4 cut(s) | 15, 125, 173, 248 |
| HpyCH4III | ACNGT | 1 cut(s) | 76 |
| HpyF3I | CTNAG | 2 cut(s) | 212, 288 |
| Hsp92II | CATG | 2 cut(s) | 179, 191 |
| LmnI | GCTCC | 1 cut(s) | 163 |
| LpnPI | CCDG | 5 cut(s) | 32, 69, 78, 96, 244 |
| Lsp1109I | GCAGC | 1 cut(s) | 232 |
| LweI | GCATC | 1 cut(s) | 14 |
| MboII | GAAGA | 1 cut(s) | 62 |
| MfeI | CAATTG | 1 cut(s) | 264 |
| MhlI | GDGCHC | 1 cut(s) | 218 |
| MluCI | AATT | 1 cut(s) | 264 |
| MlyI | GAGTC | 2 cut(s) | 112, 281 |
| MnlI | CCTC | 4 cut(s) | 140, 150, 153, 244 |
| MroXI | GAANNNNTTC | 1 cut(s) | 258 |
| MspI | CCGG | 1 cut(s) | 231 |
| MspR9I | CCNGG | 1 cut(s) | 84 |
| MunI | CAATTG | 1 cut(s) | 264 |
| Mva1269I | GAATGC | 1 cut(s) | 258 |
| MvaI | CCWGG | 1 cut(s) | 84 |
| NlaIII | CATG | 2 cut(s) | 179, 191 |
| PagI | TCATGA | 2 cut(s) | 175, 187 |
| PctI | GAATGC | 1 cut(s) | 258 |
| PdmI | GAANNNNTTC | 1 cut(s) | 258 |
| PfeI | GAWTC | 1 cut(s) | 25 |
| PkrI | GCNGC | 1 cut(s) | 247 |
| PleI | GAGTC | 2 cut(s) | 111, 280 |
| PpsI | GAGTC | 2 cut(s) | 111, 280 |
| Psp6I | CCWGG | 1 cut(s) | 82 |
| PspGI | CCWGG | 1 cut(s) | 82 |
| RsaI | GTAC | 1 cut(s) | 281 |
| RsaNI | GTAC | 1 cut(s) | 280 |
| SatI | GCNGC | 1 cut(s) | 246 |
| SchI | GAGTC | 2 cut(s) | 112, 281 |
| ScrFI | CCNGG | 1 cut(s) | 84 |
| SduI | GDGCHC | 1 cut(s) | 218 |
| SetI | ASST | 4 cut(s) | 48, 115, 126, 175 |
| SfaNI | GCATC | 1 cut(s) | 14 |
| SmlI | CTYRAG | 1 cut(s) | 114 |
| SmoI | CTYRAG | 1 cut(s) | 114 |
| Sse9I | AATT | 1 cut(s) | 264 |
| SsiI | CCGC | 3 cut(s) | 29, 64, 248 |
| StyD4I | CCNGG | 1 cut(s) | 82 |
| TaaI | ACNGT | 1 cut(s) | 76 |
| TasI | AATT | 1 cut(s) | 264 |
| TatI | WGTACW | 1 cut(s) | 279 |
| TfiI | GAWTC | 1 cut(s) | 25 |
| TseI | GCWGC | 1 cut(s) | 245 |
| TspDTI | ATGAA | 1 cut(s) | 192 |
| XmnI | GAANNNNTTC | 1 cut(s) | 258 |