Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4B

Orthogroup ID

OG0000972

Physical Location & Seq

Genomic Coordinates Forward (+)

55596609 .. 55601041

Gene Structure 4433 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4BG406100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1560 bp

ATGGCGTTGAGATCGGGCGGCCGGAAGCTCTCGTTCGAGATTCTGAGCAGGAACAGCTCCCACGAAGAAGAAGAAGAAGCCGGACTCTTCTACCGATCCAACTCAGATCCAATCCGATCGAGCCACGACACCGCCGACGATCACAAGCCCGCCGCCGCGCGAAGAAAACGCAAGAAGAAGAAGAGGACCGTCGCGAACTGCTCCATACCGGAGAGCCCCACCGCCGCGGAGGACGACGCCGTTAAGAGCGACGTGATTAACGGATTGGAATTCAATTATAGCCAGACGGTGTTGTGCTCCGTCACGACGGAGGTCGTCAGCGACCCGGAGTTTCAGAATAATTTTGGCGCGCCCGAATTGAGGCAGAGGACTGTCAATGCCGGCGCCGGAGGAGGAGTTGTGGGCGAAACGACGCCGTCCGGTGCCTTAGAAGGTGAGGCCAATGCAAAGGAGGAGAGTGGCGTCGAGGCCGCGGTGGCCAAGCCGAATGGTAATTTGGTTACGAAATTGCAGAGCGCCGAGTCGTTAGATTGGCAGCGGGTCATGGCCGAAGATCCGAATTATATGTTTTCAGTGGACAAGTCTCCACTGAAGTACTTCATGGAAGAAATGTCTAATGGGAATTCATTAAGGAGCACAACAACCCTTGGAAATGAGAAAGAAAGAGAGAGGGTTTATGATACTATCTTTCGCTTACCTTGGAGATGTGAATTGCTGATAGATGTTGGCTTTTTCGTGTGCCTTGATTCGTTTCTCTCGCTGTTGACTATCATGCCAACAAGACTTTTGATGACCCTTTGGAGGCTTGTGCAAACAAGGCAGTTTAAGAGGCCATCTGCAGCAGAGCTGTCTGATTTTGGGTGTTTTACGATAATGGCTTGTGGTGTCATTCTTTTGGAGCAAACAGATATCAGCTTAATATATCACATGATTCGTGGTCAAGGAACAATCAAATTGTATGTGGTGTACAATGTCTTGGAGATATTTGACAAACTATGTCAAAGTTTTAATGGAGATGTATTGCAAACTCTATTTAACTCGGCAGACGGACTTGCTAGCTGTCCAACAGAAAACATGAGATTCTGGATTTGGAGATTTACTTGCGACCAAGCTTTAGCCGCGATTACTTTGTCAACCTGCATTGTTGCTCACAATAATGCCTTGTTCGCTTTGCTGGTGTCCAATAACTTTGCCGAGATAAAAAGCAACGTGTTCAAGCGATATAGTAAGGAAAACATTCATAGCCTGGTATACTTTGATTCAGTGGAGAGATTCCACATTTCTGCATTTATTATATTCATCCTAGCTCAAAACATTCTGGAGGCTGAGGGTCCCTGGTTTGAAAGTTTTCTTTTTAACGCTGGTGTGGTATTCGTCTGTGAAATGATTATTGATATCGTAAAGCACTCGTTCATTGCTAAGTTCAATGACATAAAGCCCATTGCATACTCTGAGTTTCTTGAAGACCTATGCAAGCAGGTTCAGCTTCGCTCACAGCCGAAGAATTCTGCTCAAGGAACCTCAACCAACAAGTTGAAAATTGAGGTGCTGGAATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005788 GO:0006935 GO:0007275 GO:0008104 GO:0008150 GO:0009605 GO:0009790 GO:0009791 GO:0009793 GO:0009856 GO:0009987 GO:0010154 GO:0010183 GO:0012505 GO:0022414 GO:0031974 GO:0032501 GO:0032502 GO:0032507 GO:0033036 GO:0033365 GO:0034613 GO:0035437 GO:0040011 GO:0042221 GO:0042330 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0045185 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0048868 GO:0050896 GO:0050918 GO:0051179 GO:0051235 GO:0051641 GO:0051651 GO:0051704 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0070972 GO:0072595

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF747

Protein Analysis

519

Amino Acids

58.26

Weight (kDa)

5.44

Isoelectric Point (pI)

44.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF747	PF05346	280 - 504	3.6e-70	Eukaryotic membrane protein family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67960
fragaria_vesca	FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240 FvH4_4g33240
malus_domestica	MD13G1017500.v1.1 MD13G1017700.v1.1 MD16G1015700.v1.1
prunus_persica	Prupe.1G335900_v2.0.a1 Prupe.1G335900_v2.0.a1 Prupe.1G335900_v2.0.a1
pyrus_communis	pycom13g01510 pycom13g01520 pycom16g01460
rosa_chinensis	RchiOBHm_Chr2g0114371 RchiOBHm_Chr4g0390701 RchiOBHm_Chr4g0442391 RchiOBHm_Chr5g0078431 RchiOBHm_Chr6g0287871
rosa_laevigata	RLG00000017993 RLG00000020662 RLG00000032414
rosa_multiflora	Rmu_sc0000346.1_g000015 Rmu_sc0006380.1_g000001 Rmu_sc0006478.1_g000002 Rmu_sc0036628.1_g000001 Rmu_ssc0000204.1_g000063
rosa_roxburghii	Rroxscaffold_2G00081610 Rroxscaffold_3G00248840 Rroxscaffold_5G00383240 Rroxscaffold_7G00183810
rosa_rugosa	Rorug04G0340800
rosa_samantha	Rh1AG155700 Rh1AG281100 Rh1CG145900 Rh2BG511100 Rh2BG570500 Rh2CG250700 Rh2DG255000 Rh2DG522900 Rh4AG033000 Rh4AG392400 Rh4BG026900 Rh4BG406100 Rh4CG421200 Rh4DG399200 Rh5AG369200 Rh5DG164900 Rh6BG139300 Rh6CG132300 Rh6DG122800 Rh7DG359500
rosa_wichuraiana	Rw2G010190 Rw4G033890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1145, 1468
AccB1I	GGYRCC	2 cut(s)	383, 422
AccI	GTMKAC	1 cut(s)	1251
AccII	CGCG	7 cut(s)	158, 160, 194, 227, 350, 473, 1121
AclWI	GGATC	3 cut(s)	90, 101, 548
AcoI	YGGCCR	3 cut(s)	19, 477, 546
AcsI	RAATTY	3 cut(s)	269, 622, 1504
AcuI	CTGAAG	1 cut(s)	611
AcyI	GRCGYC	4 cut(s)	237, 384, 413, 462
AfaI	GTAC	2 cut(s)	596, 968
AfiI	CCNNNNNNNGG	2 cut(s)	360, 801
AflIII	ACRYGT	1 cut(s)	1209
AgsI	TTSAA	6 cut(s)	274, 1216, 1343, 1426, 1463, 1537
AjiI	CACGTC	1 cut(s)	253
AjnI	CCWGG	2 cut(s)	1245, 1334
AjuI	GAANNNNNNNTTGG	2 cut(s)	479, 511
AluBI	AGCT	8 cut(s)	28, 57, 847, 915, 1059, 1112, 1307, 1486
AluI	AGCT	8 cut(s)	28, 57, 847, 915, 1059, 1112, 1307, 1486
Alw21I	GWGCWC	2 cut(s)	299, 638
Alw26I	GTCTC	1 cut(s)	588
AlwI	GGATC	3 cut(s)	90, 101, 548
AoxI	GGCC	6 cut(s)	19, 438, 468, 477, 546, 830
ApeKI	GCWGC	2 cut(s)	535, 839
ApoI	RAATTY	3 cut(s)	269, 622, 1504
AscI	GGCGCGCC	1 cut(s)	348
Asp700I	GAANNNNTTC	3 cut(s)	596, 1236, 1347
AspLEI	GCGC	5 cut(s)	160, 350, 352, 386, 518
AspS9I	GGNCC	2 cut(s)	186, 1331
AsuC2I	CCSGG	1 cut(s)	326
AsuHPI	GGTGA	1 cut(s)	446
AsuNHI	GCTAGC	1 cut(s)	1055
AvaII	GGWCC	2 cut(s)	186, 1331
BalI	TGGCCA	1 cut(s)	479
BanI	GGYRCC	2 cut(s)	383, 422
BanII	GRGCYC	1 cut(s)	218
BbsI	GAAGAC	1 cut(s)	1470
Bbv12I	GWGCWC	2 cut(s)	299, 638
BbvCI	CCTCAGC	1 cut(s)	1326
BbvI	GCAGC	2 cut(s)	547, 851
BccI	CCATC	1 cut(s)	841
BceAI	ACGGC	2 cut(s)	224, 400
BciT130I	CCWGG	2 cut(s)	1247, 1336
BcnI	CCSGG	1 cut(s)	326
BcoDI	GTCTC	1 cut(s)	588
BfaI	CTAG	2 cut(s)	1056, 1304
BfmI	CTRYAG	1 cut(s)	837
BfoI	RGCGCY	2 cut(s)	387, 519
BfuAI	ACCTGC	2 cut(s)	1145, 1468
BglI	GCCNNNNNGGC	1 cut(s)	476
BisI	GCNGC	8 cut(s)	19, 153, 156, 225, 471, 536, 840, 1119
BlsI	GCNGC	8 cut(s)	20, 154, 157, 226, 472, 537, 841, 1120
BmcAI	AGTACT	1 cut(s)	596
Bme1390I	CCNGG	3 cut(s)	326, 1247, 1336
Bme18I	GGWCC	2 cut(s)	186, 1331
BmgBI	CACGTC	1 cut(s)	253
BmgT120I	GGNCC	2 cut(s)	186, 1331
BmiI	GGNNCC	5 cut(s)	385, 424, 1332, 1333, 1519
BmrFI	CCNGG	3 cut(s)	326, 1247, 1336
BmtI	GCTAGC	1 cut(s)	1059
BoxI	GACNNNNGTC	1 cut(s)	311
BpiI	GAAGAC	1 cut(s)	1470
BpmI	CTGGAG	1 cut(s)	1340
Bpu10I	CCTNAGC	1 cut(s)	1326
BpuEI	CTTGAG	1 cut(s)	1497
BpuMI	CCSGG	1 cut(s)	326
BsaHI	GRCGYC	4 cut(s)	237, 384, 413, 462
BsaJI	CCNNGG	5 cut(s)	225, 471, 646, 698, 1334
BsaWI	WCCGGW	2 cut(s)	208, 419
BsaXI	ACNNNNNCTCC	4 cut(s)	381, 384, 411, 414
Bsc4I	CCNNNNNNNGG	2 cut(s)	360, 801
Bse118I	RCCGGY	1 cut(s)	380
Bse3DI	GCAATG	2 cut(s)	1413, 1440
BseBI	CCWGG	2 cut(s)	1247, 1336
BseDI	CCNNGG	5 cut(s)	225, 471, 646, 698, 1334
BseGI	GGATG	1 cut(s)	1299
BseLI	CCNNNNNNNGG	2 cut(s)	360, 801
BseMI	GCAATG	2 cut(s)	1413, 1440
BseMII	CTCAG	4 cut(s)	35, 117, 1317, 1443
BsePI	GCGCGC	1 cut(s)	348
BseRI	GAGGAG	3 cut(s)	405, 408, 467
BseX3I	CGGCCG	1 cut(s)	19
BseXI	GCAGC	2 cut(s)	547, 851
Bsh1236I	CGCG	7 cut(s)	158, 160, 194, 227, 350, 473, 1121
Bsh1285I	CGRYCG	2 cut(s)	22, 119
BshFI	GGCC	6 cut(s)	21, 440, 470, 479, 548, 832
BshNI	GGYRCC	2 cut(s)	383, 422
BsiEI	CGRYCG	2 cut(s)	22, 119
BsiHKAI	GWGCWC	2 cut(s)	299, 638
BsiSI	CCGG	7 cut(s)	22, 81, 209, 326, 381, 387, 420
BslFI	GGGAC	1 cut(s)	1317
BslI	CCNNNNNNNGG	2 cut(s)	360, 801
BsmAI	GTCTC	1 cut(s)	588
BsmFI	GGGAC	1 cut(s)	1317
BsnI	GGCC	6 cut(s)	21, 440, 470, 479, 548, 832
Bsp1286I	GDGCHC	3 cut(s)	218, 299, 638
Bsp1407I	TGTACA	1 cut(s)	966
Bsp143I	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
Bsp68I	TCGCGA	1 cut(s)	194
BspANI	GGCC	6 cut(s)	21, 440, 470, 479, 548, 832
BspCNI	CTCAG	4 cut(s)	36, 116, 1318, 1444
BspFNI	CGCG	7 cut(s)	158, 160, 194, 227, 350, 473, 1121
BspLI	GGNNCC	5 cut(s)	385, 424, 1332, 1333, 1519
BspMAI	CTGCAG	1 cut(s)	841
BspMI	ACCTGC	2 cut(s)	1145, 1468
BspOI	GCTAGC	1 cut(s)	1059
BspPI	GGATC	3 cut(s)	90, 101, 548
BspT107I	GGYRCC	2 cut(s)	383, 422
BsrDI	GCAATG	2 cut(s)	1413, 1440
BsrFI	RCCGGY	1 cut(s)	380
BsrGI	TGTACA	1 cut(s)	966
BssAI	RCCGGY	1 cut(s)	380
BssECI	CCNNGG	5 cut(s)	225, 471, 646, 698, 1334
BssHII	GCGCGC	1 cut(s)	348
BssMI	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
BssNAI	GTATAC	1 cut(s)	1252
BssNI	GRCGYC	4 cut(s)	237, 384, 413, 462
BssT1I	CCWWGG	2 cut(s)	646, 698
Bst1107I	GTATAC	1 cut(s)	1252
Bst2UI	CCWGG	2 cut(s)	1247, 1336
Bst4CI	ACNGT	3 cut(s)	190, 289, 373
Bst6I	CTCTTC	2 cut(s)	92, 176
BstACI	GRCGYC	4 cut(s)	237, 384, 413, 462
BstAUI	TGTACA	1 cut(s)	966
BstC8I	GCNNGC	5 cut(s)	150, 350, 382, 1057, 1475
BstDEI	CTNAG	6 cut(s)	44, 103, 427, 1326, 1419, 1452
BstDSI	CCRYGG	2 cut(s)	225, 471
BstF5I	GGATG	1 cut(s)	1299
BstFNI	CGCG	7 cut(s)	158, 160, 194, 227, 350, 473, 1121
BstH2I	RGCGCY	2 cut(s)	387, 519
BstHHI	GCGC	5 cut(s)	160, 350, 352, 386, 518
BstKTI	GATC	6 cut(s)	14, 98, 109, 119, 142, 556
BstMAI	GTCTC	1 cut(s)	588
BstMBI	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
BstMCI	CGRYCG	2 cut(s)	22, 119
BstMWI	GCNNNNNNNGC	6 cut(s)	54, 476, 817, 1118, 1166, 1483
BstNI	CCWGG	2 cut(s)	1247, 1336
BstPAI	GACNNNNGTC	1 cut(s)	311
BstSCI	CCNGG	3 cut(s)	324, 1245, 1334
BstSFI	CTRYAG	1 cut(s)	837
BstUI	CGCG	7 cut(s)	158, 160, 194, 227, 350, 473, 1121
BstV1I	GCAGC	2 cut(s)	547, 851
BstV2I	GAAGAC	1 cut(s)	1470
BstX2I	RGATCY	2 cut(s)	106, 553
BstYI	RGATCY	2 cut(s)	106, 553
BstZ17I	GTATAC	1 cut(s)	1252
BstZI	CGGCCG	1 cut(s)	19
BsuRI	GGCC	6 cut(s)	21, 440, 470, 479, 548, 832
BtgI	CCRYGG	2 cut(s)	225, 471
BtrI	CACGTC	1 cut(s)	253
BtsCI	GGATG	1 cut(s)	1299
BtsIMutI	CAGTG	3 cut(s)	579, 587, 1269
BtuMI	TCGCGA	1 cut(s)	194
BveI	ACCTGC	2 cut(s)	1145, 1468
Cac8I	GCNNGC	5 cut(s)	150, 350, 382, 1057, 1475
CfoI	GCGC	5 cut(s)	160, 350, 352, 386, 518
Cfr10I	RCCGGY	1 cut(s)	380
Cfr13I	GGNCC	2 cut(s)	186, 1331
Cfr42I	CCGCGG	2 cut(s)	228, 474
CseI	GACGC	3 cut(s)	245, 421, 451
Csp6I	GTAC	2 cut(s)	595, 967
CviAII	CATG	5 cut(s)	544, 601, 772, 928, 1075
CviQI	GTAC	2 cut(s)	595, 967
DdeI	CTNAG	6 cut(s)	44, 103, 427, 1326, 1419, 1452
DinI	GGCGCC	1 cut(s)	385
DpnI	GATC	6 cut(s)	13, 97, 108, 118, 141, 555
DpnII	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
EaeI	YGGCCR	3 cut(s)	19, 477, 546
EagI	CGGCCG	1 cut(s)	19
Eam1104I	CTCTTC	2 cut(s)	92, 176
EarI	CTCTTC	2 cut(s)	92, 176
EclXI	CGGCCG	1 cut(s)	19
Eco130I	CCWWGG	2 cut(s)	646, 698
Eco24I	GRGCYC	1 cut(s)	218
Eco32I	GATATC	2 cut(s)	910, 1396
Eco47I	GGWCC	2 cut(s)	186, 1331
Eco52I	CGGCCG	1 cut(s)	19
Eco57I	CTGAAG	1 cut(s)	611
EcoO109I	RGGNCCY	1 cut(s)	1331
EcoRI	GAATTC	3 cut(s)	269, 622, 1504
EcoRII	CCWGG	2 cut(s)	1245, 1334
EcoRV	GATATC	2 cut(s)	910, 1396
EcoT14I	CCWWGG	2 cut(s)	646, 698
EcoT38I	GRGCYC	1 cut(s)	218
EgeI	GGCGCC	1 cut(s)	385
EheI	GGCGCC	1 cut(s)	385
ErhI	CCWWGG	2 cut(s)	646, 698
FaeI	CATG	5 cut(s)	547, 604, 775, 931, 1078
FaqI	GGGAC	1 cut(s)	1317
FatI	CATG	5 cut(s)	543, 600, 771, 927, 1074
FauI	CCCGC	2 cut(s)	157, 531
FblI	GTMKAC	1 cut(s)	1251
Fnu4HI	GCNGC	8 cut(s)	19, 153, 156, 225, 471, 536, 840, 1119
FokI	GGATG	1 cut(s)	1286
FriOI	GRGCYC	1 cut(s)	218
Fsp4HI	GCNGC	8 cut(s)	19, 153, 156, 225, 471, 536, 840, 1119
FspBI	CTAG	2 cut(s)	1056, 1304
GlaI	GCGC	5 cut(s)	159, 349, 351, 385, 517
GluI	GCNGC	8 cut(s)	19, 153, 156, 225, 471, 536, 840, 1119
GsuI	CTGGAG	1 cut(s)	1340
HaeII	RGCGCY	2 cut(s)	387, 519
HaeIII	GGCC	6 cut(s)	21, 440, 470, 479, 548, 832
HapII	CCGG	7 cut(s)	22, 81, 209, 326, 381, 387, 420
HgaI	GACGC	3 cut(s)	245, 421, 451
HhaI	GCGC	5 cut(s)	160, 350, 352, 386, 518
Hin1I	GRCGYC	4 cut(s)	237, 384, 413, 462
Hin1II	CATG	5 cut(s)	547, 604, 775, 931, 1078
Hin6I	GCGC	5 cut(s)	158, 348, 350, 384, 516
HinP1I	GCGC	5 cut(s)	158, 348, 350, 384, 516
HincII	GTYRAC	2 cut(s)	765, 1134
HindII	GTYRAC	2 cut(s)	765, 1134
HindIII	AAGCTT	1 cut(s)	1110
HinfI	GANTC	8 cut(s)	40, 84, 521, 746, 931, 1080, 1259, 1272
HpaII	CCGG	7 cut(s)	22, 81, 209, 326, 381, 387, 420
HphI	GGTGA	1 cut(s)	446
Hpy166II	GTNNAC	5 cut(s)	577, 765, 967, 1134, 1252
Hpy188I	TCNGA	7 cut(s)	45, 106, 116, 336, 558, 853, 1453
Hpy188III	TCNNGA	6 cut(s)	37, 193, 304, 1084, 1319, 1460
Hpy8I	GTNNAC	5 cut(s)	577, 765, 967, 1134, 1252
Hpy99I	CGWCG	7 cut(s)	140, 194, 239, 254, 310, 415, 467
HpyAV	CCTTC	1 cut(s)	425
HpyCH4III	ACNGT	3 cut(s)	190, 289, 373
HpyCH4IV	ACGT	2 cut(s)	252, 1209
HpyCH4V	TGCA	9 cut(s)	446, 511, 811, 839, 1024, 1140, 1286, 1445, 1473
HpyF10VI	GCNNNNNNNGC	6 cut(s)	54, 476, 817, 1118, 1166, 1483
HpyF3I	CTNAG	6 cut(s)	44, 103, 427, 1326, 1419, 1452
HpySE526I	ACGT	2 cut(s)	252, 1209
Hsp92I	GRCGYC	4 cut(s)	237, 384, 413, 462
Hsp92II	CATG	5 cut(s)	547, 604, 775, 931, 1078
HspAI	GCGC	5 cut(s)	158, 348, 350, 384, 516
KasI	GGCGCC	1 cut(s)	383
KflI	GGGWCCC	1 cut(s)	1331
KroI	GCCGGC	1 cut(s)	380
KroNI	GCCGGC	1 cut(s)	382
KspI	CCGCGG	2 cut(s)	228, 474
Kzo9I	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
LmnI	GCTCC	5 cut(s)	62, 206, 302, 633, 898
Lsp1109I	GCAGC	2 cut(s)	547, 851
MaeI	CTAG	2 cut(s)	1056, 1304
MaeII	ACGT	2 cut(s)	252, 1209
MaeIII	GTNAC	2 cut(s)	301, 499
MalI	GATC	6 cut(s)	13, 97, 108, 118, 141, 555
MboI	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
MflI	RGATCY	2 cut(s)	106, 553
MhlI	GDGCHC	3 cut(s)	218, 299, 638
MlsI	TGGCCA	1 cut(s)	479
MluNI	TGGCCA	1 cut(s)	479
Mly113I	GGCGCC	1 cut(s)	384
MlyI	GAGTC	2 cut(s)	78, 530
MmeI	TCCRAC	2 cut(s)	123, 1088
Mox20I	TGGCCA	1 cut(s)	479
MroNI	GCCGGC	1 cut(s)	380
MroXI	GAANNNNTTC	3 cut(s)	596, 1236, 1347
MscI	TGGCCA	1 cut(s)	479
MseI	TTAA	8 cut(s)	243, 258, 629, 825, 917, 1008, 1035, 1356
MslI	CAYNNNNRTG	1 cut(s)	1155
Msp20I	TGGCCA	1 cut(s)	479
MspA1I	CMGCKG	3 cut(s)	227, 473, 538
MspI	CCGG	7 cut(s)	22, 81, 209, 326, 381, 387, 420
MspR9I	CCNGG	3 cut(s)	326, 1247, 1336
MvaI	CCWGG	2 cut(s)	1247, 1336
MvnI	CGCG	7 cut(s)	158, 160, 194, 227, 350, 473, 1121
MwoI	GCNNNNNNNGC	6 cut(s)	54, 476, 817, 1118, 1166, 1483
NaeI	GCCGGC	1 cut(s)	382
NarI	GGCGCC	1 cut(s)	384
NciI	CCSGG	1 cut(s)	326
NdeII	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
NgoMIV	GCCGGC	1 cut(s)	380
NheI	GCTAGC	1 cut(s)	1055
NlaIII	CATG	5 cut(s)	547, 604, 775, 931, 1078
NlaIV	GGNNCC	5 cut(s)	385, 424, 1332, 1333, 1519
NmeAIII	GCCGAG	3 cut(s)	544, 1019, 1219
NmuCI	GTSAC	1 cut(s)	301
NruI	TCGCGA	1 cut(s)	194
PalAI	GGCGCGCC	1 cut(s)	348
PauI	GCGCGC	1 cut(s)	348
PcsI	WCGNNNNNNNCGW	1 cut(s)	132
PdiI	GCCGGC	1 cut(s)	382
PdmI	GAANNNNTTC	3 cut(s)	596, 1236, 1347
PfeI	GAWTC	6 cut(s)	40, 746, 931, 1080, 1259, 1272
PflFI	GACNNNGTC	1 cut(s)	415
PkrI	GCNGC	8 cut(s)	20, 154, 157, 226, 472, 537, 841, 1120
Ple19I	CGATCG	1 cut(s)	119
PleI	GAGTC	2 cut(s)	78, 529
PluTI	GGCGCC	1 cut(s)	387
PpsI	GAGTC	2 cut(s)	78, 529
PpuMI	RGGWCCY	1 cut(s)	1331
PshAI	GACNNNNGTC	1 cut(s)	311
Psp5II	RGGWCCY	1 cut(s)	1331
Psp6I	CCWGG	2 cut(s)	1245, 1334
PspGI	CCWGG	2 cut(s)	1245, 1334
PspN4I	GGNNCC	5 cut(s)	385, 424, 1332, 1333, 1519
PspPI	GGNCC	2 cut(s)	186, 1331
PspPPI	RGGWCCY	1 cut(s)	1331
PstI	CTGCAG	1 cut(s)	841
PsuI	RGATCY	2 cut(s)	106, 553
PsyI	GACNNNGTC	1 cut(s)	415
PteI	GCGCGC	1 cut(s)	348
PvuI	CGATCG	1 cut(s)	119
RruI	TCGCGA	1 cut(s)	194
RsaI	GTAC	2 cut(s)	596, 968
RsaNI	GTAC	2 cut(s)	595, 967
RseI	CAYNNNNRTG	1 cut(s)	1155
SacII	CCGCGG	2 cut(s)	228, 474
SaqAI	TTAA	8 cut(s)	243, 258, 629, 825, 917, 1008, 1035, 1356
SatI	GCNGC	8 cut(s)	19, 153, 156, 225, 471, 536, 840, 1119
Sau3AI	GATC	6 cut(s)	11, 95, 106, 116, 139, 553
Sau96I	GGNCC	2 cut(s)	186, 1331
ScaI	AGTACT	1 cut(s)	596
SchI	GAGTC	2 cut(s)	78, 530
ScrFI	CCNGG	3 cut(s)	326, 1247, 1336
SduI	GDGCHC	3 cut(s)	218, 299, 638
SfcI	CTRYAG	1 cut(s)	837
SfiI	GGCCNNNNNGGCC	1 cut(s)	476
SfoI	GGCGCC	1 cut(s)	385
Sfr303I	CCGCGG	2 cut(s)	228, 474
SgrBI	CCGCGG	2 cut(s)	228, 474
SgsI	GGCGCGCC	1 cut(s)	348
SinI	GGWCC	2 cut(s)	186, 1331
SmiMI	CAYNNNNRTG	1 cut(s)	1155
SmlI	CTYRAG	1 cut(s)	1512
SmoI	CTYRAG	1 cut(s)	1512
SspDI	GGCGCC	1 cut(s)	383
SspMI	CTAG	2 cut(s)	1056, 1304
StyD4I	CCNGG	3 cut(s)	324, 1245, 1334
StyI	CCWWGG	2 cut(s)	646, 698
TaaI	ACNGT	3 cut(s)	190, 289, 373
TaiI	ACGT	2 cut(s)	255, 1212
TaqI	TCGA	3 cut(s)	36, 119, 465
TatI	WGTACW	2 cut(s)	594, 966
TauI	GCSGC	6 cut(s)	21, 155, 158, 227, 473, 1121
TfiI	GAWTC	6 cut(s)	40, 746, 931, 1080, 1259, 1272
Tru1I	TTAA	8 cut(s)	243, 258, 629, 825, 917, 1008, 1035, 1356
Tru9I	TTAA	8 cut(s)	243, 258, 629, 825, 917, 1008, 1035, 1356
TscAI	CASTG	3 cut(s)	579, 594, 1269
TseFI	GTSAC	1 cut(s)	301
TseI	GCWGC	2 cut(s)	535, 839
Tsp45I	GTSAC	1 cut(s)	301
TspDTI	ATGAA	5 cut(s)	589, 615, 1229, 1288, 1402
TspGWI	ACGGA	4 cut(s)	276, 289, 323, 1062
TspRI	CASTG	3 cut(s)	579, 594, 1269
Tth111I	GACNNNGTC	1 cut(s)	415
VpaK11BI	GGWCC	2 cut(s)	186, 1331
XapI	RAATTY	3 cut(s)	269, 622, 1504
XmiI	GTMKAC	1 cut(s)	1251
XmnI	GAANNNNTTC	3 cut(s)	596, 1236, 1347
XspI	CTAG	2 cut(s)	1056, 1304
ZrmI	AGTACT	1 cut(s)	596

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.