AT1G33720

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001080
Physical Location & Seq
Reverse (-)
12220858 .. 12224108
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G33720.4

Sequence Viewer

Length: 1536 bp
ATGGACATCATCTCAGGACAACCTCTATTTCTCATCTTCTGCTTTATCTTATCATGTCTTCTTTTCTTCACCACCGCACGATCTCGACGTAGCCCTTGTCAATTGTCCAAGTCACCTCCGGGGCCTCCACGGTTACCCATAATTGGAAACATTCATCTGGTCGGAAAGAACCCACATCACTCATTCACGGACCTCTCAAAAACATATGGACCAGTCATGAGTCTTAAGCTTGGATGTTTAAACTCAGTGGTCATAGCTTCACGAGACGCTGTGAGAGAAGTACTAAAAACACACGACCAAATCTTGTCTGGACGGTACATAAGTGAAGCGACCAAATCCAACAATCATCATGAATTTTCCGTTGGCTGGATTCATCCATCGTCATCTCGTTTTAGAATGTTGAGGAAATTGTCGGCTACTCAGCTGTTCTCACCGCAGTGTATCCAGGCTACCAAAGCTCTGAGGATGAAGAAGGTGCAGGAACTTGTCAACTTCCTGAGTGAAAGCTGTGAGAGAGAAGAAGCTGTTGATATTTCTCATGTATCCTTCGTCACAGCTCTCAATATCATATCGAATATTTTGTTCTCAGTCAATCTCGGTAGCTACGATTCAAAAAATTCCAGTGCGTTTCAAGAGATGGTGATCGGTTACCAGGAATCTATCGGAAATCCAGACCTTGCTAACTTCTTCCCATTTATGAGATTTCTTGACTTGCAAGGTAACAGTAAGAAGATGAGGGAGTCCTCAGGGAGATTGCTTCAGGTATTCAGAGAGTTTTATGATGCTCGGATTGTGGAGAAATCATCGCGTTCAGTTGAGAAAGATGTTTCGAGTAAAGACTTCCTGGATGTGCTTATCGATCTCCAACAAGGAGATGAAACTGAAATCAACATCGACGAGATCGAACACCTACTTCTCGATATGTTTGTAGCAGGCACAGATACAAACTCAAGTACCGTGGAATGGGCAATGGCAGAGTTACTTGGAAACCCTAAAACGATGACAAAAGTTCAAGACGAGATCAATCATGTGATAGGGCAAAACGGTGACTTTCAAGAGTCTGATATCTCAAAACTGCCGTATTTAAAAGCGGTCGTAAAAGAAACTTTCCGACTACACCCTGCTGCTCCATTTCTCCTCCAAAGAAAAGCTGAAACCAACGTAGAGATTCTTGGATTCACTGTGCTTAAAGACTCTCAGGTTCTTGTGAACGTGTGGGCCATAGGACGAGACCCACTTGTATGGGAAAATCCGACCCATTTTGAGCCAGAGAGGTTTTTGGGGAAAGAGATTGATGTAAAAGGTACAGATTATGAGCTTACACCGTTTGGGGCTGGGCGTAGAATTTGTCCGGGACTGCCTTTGGCTATGAAAACAGTGCATCTCATGCTTGCTTCTCTTCTCTATACCTTTGAATGGAAGCTTCCAAACGGTGTCGGTTCAGAGGATTTGGACATGGAAGAGACCTTTGGTCTCACAGTGCACAAGACAAACCCGTTACTTGCCGTGCCTCTGAAGAAACGAGCCATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

511

Amino Acids

57.48

Weight (kDa)

6.3

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 365 6.4e-87 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 808
AciI CCGC 3 cut(s) 75, 434, 1091
AcsI RAATTY 3 cut(s) 353, 616, 1344
AcuI CTGAAG 2 cut(s) 743, 1535
AfaI GTAC 4 cut(s) 282, 317, 955, 1306
AfiI CCNNNNNNNGG 4 cut(s) 143, 366, 963, 1416
AflII CTTAAG 1 cut(s) 224
AflIII ACRYGT 1 cut(s) 1212
AgsI TTSAA 5 cut(s) 612, 632, 1013, 1055, 1415
AhdI GACNNNNNGTC 1 cut(s) 1470
AjnI CCWGG 3 cut(s) 444, 651, 843
AjuI GAANNNNNNNTTGG 4 cut(s) 345, 377, 1452, 1484
AleI CACNNNNGTG 1 cut(s) 436
Alw21I GWGCWC 1 cut(s) 1485
Alw26I GTCTC 4 cut(s) 258, 1224, 1457, 1478
Alw44I GTGCAC 1 cut(s) 1481
AoxI GGCC 2 cut(s) 122, 1218
ApaLI GTGCAC 1 cut(s) 1481
ApeKI GCWGC 1 cut(s) 1124
ApoI RAATTY 3 cut(s) 353, 616, 1344
AspS9I GGNCC 4 cut(s) 122, 190, 209, 1218
AsuC2I CCSGG 2 cut(s) 120, 1353
AsuHPI GGTGA 5 cut(s) 61, 105, 423, 652, 1058
AvaII GGWCC 2 cut(s) 190, 209
AxyI CCTNAGG 1 cut(s) 745
BaeGI GKGCMC 1 cut(s) 1485
BaeI ACNNNNGTAYC 4 cut(s) 307, 340, 937, 970
BauI CACGAG 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 1485
BbvI GCAGC 1 cut(s) 1111
BccI CCATC 2 cut(s) 385, 631
BceAI ACGGC 2 cut(s) 1063, 1490
BciT130I CCWGG 3 cut(s) 446, 653, 845
BciVI GTATCC 2 cut(s) 452, 553
BcnI CCSGG 2 cut(s) 120, 1353
BcoDI GTCTC 4 cut(s) 258, 1224, 1457, 1478
BfrI CTTAAG 1 cut(s) 224
BfuI GTATCC 2 cut(s) 452, 553
BisI GCNGC 1 cut(s) 1125
BlsI GCNGC 1 cut(s) 1126
BmcAI AGTACT 1 cut(s) 282
Bme1390I CCNGG 5 cut(s) 120, 446, 653, 845, 1353
Bme18I GGWCC 2 cut(s) 190, 209
BmeRI GACNNNNNGTC 1 cut(s) 1470
BmgT120I GGNCC 4 cut(s) 122, 190, 209, 1218
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 5 cut(s) 120, 446, 653, 845, 1353
BmsI GCATC 2 cut(s) 772, 1390
BpiI GAAGAC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 934
BpuMI CCSGG 2 cut(s) 120, 1353
Bsa29I ATCGAT 1 cut(s) 858
BsaBI GATNNNNATC 1 cut(s) 641
BsaI GGTCTC 3 cut(s) 1224, 1457, 1478
BsaJI CCNNGG 3 cut(s) 119, 128, 957
Bsc4I CCNNNNNNNGG 4 cut(s) 143, 366, 963, 1416
Bse1I ACTGG 2 cut(s) 212, 621
Bse21I CCTNAGG 1 cut(s) 745
Bse3DI GCAATG 1 cut(s) 975
Bse8I GATNNNNATC 1 cut(s) 641
BseBI CCWGG 3 cut(s) 446, 653, 845
BseCI ATCGAT 1 cut(s) 858
BseDI CCNNGG 3 cut(s) 119, 128, 957
BseGI GGATG 4 cut(s) 239, 373, 471, 853
BseJI GATNNNNATC 1 cut(s) 641
BseLI CCNNNNNNNGG 4 cut(s) 143, 366, 963, 1416
BseMI GCAATG 1 cut(s) 975
BseMII CTCAG 8 cut(s) 27, 258, 434, 452, 488, 600, 759, 1211
BseNI ACTGG 2 cut(s) 212, 621
BseRI GAGGAG 1 cut(s) 1127
BseSI GKGCMC 1 cut(s) 1485
BseXI GCAGC 1 cut(s) 1111
BseYI CCCAGC 1 cut(s) 1334
BsgI GTGCAG 1 cut(s) 497
Bsh1236I CGCG 1 cut(s) 808
Bsh1285I CGRYCG 1 cut(s) 1095
BshFI GGCC 2 cut(s) 124, 1220
BshVI ATCGAT 1 cut(s) 858
BsiEI CGRYCG 1 cut(s) 1095
BsiHKAI GWGCWC 1 cut(s) 1485
BsiSI CCGG 2 cut(s) 119, 1352
BslFI GGGAC 1 cut(s) 1368
BslI CCNNNNNNNGG 4 cut(s) 143, 366, 963, 1416
BsmAI GTCTC 4 cut(s) 258, 1224, 1457, 1478
BsmBI CGTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 1368
BsnI GGCC 2 cut(s) 124, 1220
Bso31I GGTCTC 3 cut(s) 1224, 1457, 1478
Bsp1286I GDGCHC 1 cut(s) 1485
Bsp143I GATC 5 cut(s) 80, 642, 859, 900, 1020
BspACI CCGC 3 cut(s) 75, 434, 1091
BspANI GGCC 2 cut(s) 124, 1220
BspCNI CTCAG 8 cut(s) 26, 257, 433, 453, 489, 599, 758, 1210
BspDI ATCGAT 1 cut(s) 858
BspFNI CGCG 1 cut(s) 808
BspHI TCATGA 2 cut(s) 216, 349
BspLI GGNNCC 1 cut(s) 123
BspTI CTTAAG 1 cut(s) 224
BspTNI GGTCTC 3 cut(s) 1224, 1457, 1478
BsrDI GCAATG 1 cut(s) 975
BsrI ACTGG 2 cut(s) 212, 621
BssECI CCNNGG 3 cut(s) 119, 128, 957
BssMI GATC 5 cut(s) 80, 642, 859, 900, 1020
BssSI CACGAG 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 261
Bst2UI CCWGG 3 cut(s) 446, 653, 845
Bst6I CTCTTC 2 cut(s) 1404, 1455
BstAFI CTTAAG 1 cut(s) 224
BstAPI GCANNNNNTGC 1 cut(s) 1387
BstC8I GCNNGC 2 cut(s) 934, 1392
BstDEI CTNAG 8 cut(s) 13, 244, 420, 461, 497, 586, 745, 1197
BstDSI CCRYGG 2 cut(s) 128, 957
BstEII GGTNACC 2 cut(s) 132, 647
BstF5I GGATG 4 cut(s) 239, 373, 471, 853
BstFNI CGCG 1 cut(s) 808
BstKTI GATC 5 cut(s) 83, 645, 862, 903, 1023
BstMAI GTCTC 4 cut(s) 258, 1224, 1457, 1478
BstMBI GATC 5 cut(s) 80, 642, 859, 900, 1020
BstMCI CGRYCG 1 cut(s) 1095
BstMWI GCNNNNNNNGC 2 cut(s) 455, 1387
BstNI CCWGG 3 cut(s) 446, 653, 845
BstPI GGTNACC 2 cut(s) 132, 647
BstSCI CCNGG 5 cut(s) 118, 444, 651, 843, 1351
BstSLI GKGCMC 1 cut(s) 1485
BstUI CGCG 1 cut(s) 808
BstV1I GCAGC 1 cut(s) 1111
BstV2I GAAGAC 1 cut(s) 50
BstXI CCANNNNNNTGG 1 cut(s) 1242
Bsu15I ATCGAT 1 cut(s) 858
Bsu36I CCTNAGG 1 cut(s) 745
BsuI GTATCC 2 cut(s) 452, 553
BsuRI GGCC 2 cut(s) 124, 1220
BsuTUI ATCGAT 1 cut(s) 858
BtgI CCRYGG 2 cut(s) 128, 957
BtgZI GCGATG 1 cut(s) 789
BtsCI GGATG 4 cut(s) 239, 373, 471, 853
BtsI GCAGTG 1 cut(s) 443
BtsIMutI CAGTG 6 cut(s) 252, 443, 628, 1179, 1383, 1485
Cac8I GCNNGC 2 cut(s) 934, 1392
CciI TCATGA 2 cut(s) 216, 349
Cfr13I GGNCC 4 cut(s) 122, 190, 209, 1218
ClaI ATCGAT 1 cut(s) 858
CseI GACGC 1 cut(s) 275
Csp6I GTAC 4 cut(s) 281, 316, 954, 1305
CspCI CAANNNNNGTGG 2 cut(s) 939, 974
CviAII CATG 7 cut(s) 54, 217, 350, 539, 1028, 1387, 1456
CviQI GTAC 4 cut(s) 281, 316, 954, 1305
DdeI CTNAG 8 cut(s) 13, 244, 420, 461, 497, 586, 745, 1197
DpnI GATC 5 cut(s) 82, 644, 861, 902, 1022
DpnII GATC 5 cut(s) 80, 642, 859, 900, 1020
DraI TTTAAA 2 cut(s) 240, 1086
DriI GACNNNNNGTC 1 cut(s) 1470
Eam1104I CTCTTC 2 cut(s) 1404, 1455
Eam1105I GACNNNNNGTC 1 cut(s) 1470
EarI CTCTTC 2 cut(s) 1404, 1455
Eco31I GGTCTC 3 cut(s) 1224, 1457, 1478
Eco32I GATATC 1 cut(s) 1066
Eco47I GGWCC 2 cut(s) 190, 209
Eco57I CTGAAG 2 cut(s) 743, 1535
Eco81I CCTNAGG 1 cut(s) 745
Eco91I GGTNACC 2 cut(s) 132, 647
EcoO109I RGGNCCY 1 cut(s) 122
EcoO65I GGTNACC 2 cut(s) 132, 647
EcoRII CCWGG 3 cut(s) 444, 651, 843
EcoRV GATATC 1 cut(s) 1066
Esp3I CGTCTC 1 cut(s) 258
FaeI CATG 7 cut(s) 57, 220, 353, 542, 1031, 1390, 1459
FaqI GGGAC 1 cut(s) 1368
FatI CATG 7 cut(s) 53, 216, 349, 538, 1027, 1386, 1455
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 1 cut(s) 1125
FokI GGATG 4 cut(s) 246, 360, 478, 860
Fsp4HI GCNGC 1 cut(s) 1125
GluI GCNGC 1 cut(s) 1125
GsaI CCCAGC 1 cut(s) 1338
HaeIII GGCC 2 cut(s) 124, 1220
HapII CCGG 2 cut(s) 119, 1352
HgaI GACGC 1 cut(s) 275
Hin1II CATG 7 cut(s) 57, 220, 353, 542, 1031, 1390, 1459
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 2 cut(s) 227, 1421
HinfI GANTC 9 cut(s) 220, 370, 608, 656, 740, 1058, 1168, 1176, 1193
HpaII CCGG 2 cut(s) 119, 1352
HphI GGTGA 5 cut(s) 61, 105, 423, 652, 1058
Hpy166II GTNNAC 3 cut(s) 490, 1210, 1483
Hpy8I GTNNAC 3 cut(s) 490, 1210, 1483
Hpy99I CGWCG 2 cut(s) 90, 899
HpyAV CCTTC 2 cut(s) 466, 556
HpyCH4IV ACGT 3 cut(s) 88, 1161, 1212
HpyCH4V TGCA 4 cut(s) 478, 715, 1381, 1483
HpyF10VI GCNNNNNNNGC 2 cut(s) 455, 1387
HpyF3I CTNAG 8 cut(s) 13, 244, 420, 461, 497, 586, 745, 1197
HpySE526I ACGT 3 cut(s) 88, 1161, 1212
Hsp92II CATG 7 cut(s) 57, 220, 353, 542, 1031, 1390, 1459
Kzo9I GATC 5 cut(s) 80, 642, 859, 900, 1020
LmnI GCTCC 1 cut(s) 1132
Lsp1109I GCAGC 1 cut(s) 1111
LweI GCATC 2 cut(s) 772, 1390
MaeII ACGT 3 cut(s) 88, 1161, 1212
MaeIII GTNAC 8 cut(s) 111, 132, 550, 647, 719, 978, 1046, 1497
MalI GATC 5 cut(s) 82, 644, 861, 902, 1022
MboI GATC 5 cut(s) 80, 642, 859, 900, 1020
MfeI CAATTG 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 1485
MluCI AATT 7 cut(s) 101, 141, 353, 407, 616, 1344, 1531
MlyI GAGTC 4 cut(s) 229, 749, 1067, 1187
MmeI TCCRAC 5 cut(s) 142, 363, 889, 1135, 1277
MseI TTAA 5 cut(s) 225, 239, 1085, 1188, 1534
MslI CAYNNNNRTG 2 cut(s) 436, 1240
MspA1I CMGCKG 1 cut(s) 424
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 2 cut(s) 119, 1352
MspR9I CCNGG 5 cut(s) 120, 446, 653, 845, 1353
MssI GTTTAAAC 1 cut(s) 240
MunI CAATTG 1 cut(s) 101
MvaI CCWGG 3 cut(s) 446, 653, 845
MvnI CGCG 1 cut(s) 808
MwoI GCNNNNNNNGC 2 cut(s) 455, 1387
NciI CCSGG 2 cut(s) 120, 1353
NdeI CATATG 1 cut(s) 205
NdeII GATC 5 cut(s) 80, 642, 859, 900, 1020
NlaIII CATG 7 cut(s) 57, 220, 353, 542, 1031, 1390, 1459
NlaIV GGNNCC 1 cut(s) 123
NmuCI GTSAC 3 cut(s) 111, 550, 1046
OliI CACNNNNGTG 1 cut(s) 436
PagI TCATGA 2 cut(s) 216, 349
PcsI WCGNNNNNNNCGW 3 cut(s) 85, 603, 900
PfeI GAWTC 5 cut(s) 370, 608, 656, 1168, 1176
PfoI TCCNGGA 2 cut(s) 843, 1351
PkrI GCNGC 1 cut(s) 1126
PleI GAGTC 4 cut(s) 228, 748, 1066, 1187
PmeI GTTTAAAC 1 cut(s) 240
PpsI GAGTC 4 cut(s) 228, 748, 1066, 1187
Psp6I CCWGG 3 cut(s) 444, 651, 843
PspEI GGTNACC 2 cut(s) 132, 647
PspFI CCCAGC 1 cut(s) 1334
PspGI CCWGG 3 cut(s) 444, 651, 843
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 4 cut(s) 122, 190, 209, 1218
PvuII CAGCTG 1 cut(s) 424
RsaI GTAC 4 cut(s) 282, 317, 955, 1306
RsaNI GTAC 4 cut(s) 281, 316, 954, 1305
RseI CAYNNNNRTG 2 cut(s) 436, 1240
SaqAI TTAA 5 cut(s) 225, 239, 1085, 1188, 1534
SatI GCNGC 1 cut(s) 1125
Sau3AI GATC 5 cut(s) 80, 642, 859, 900, 1020
Sau96I GGNCC 4 cut(s) 122, 190, 209, 1218
ScaI AGTACT 1 cut(s) 282
SchI GAGTC 4 cut(s) 229, 749, 1067, 1187
ScrFI CCNGG 5 cut(s) 120, 446, 653, 845, 1353
SduI GDGCHC 1 cut(s) 1485
SfaNI GCATC 2 cut(s) 772, 1390
SinI GGWCC 2 cut(s) 190, 209
SmiMI CAYNNNNRTG 2 cut(s) 436, 1240
SmlI CTYRAG 2 cut(s) 224, 949
SmoI CTYRAG 2 cut(s) 224, 949
Sse9I AATT 7 cut(s) 101, 141, 353, 407, 616, 1344, 1531
SsiI CCGC 3 cut(s) 75, 434, 1091
SspI AATATT 1 cut(s) 577
StyD4I CCNGG 5 cut(s) 118, 444, 651, 843, 1351
TaiI ACGT 3 cut(s) 91, 1164, 1215
TaqI TCGA 7 cut(s) 85, 572, 830, 858, 894, 903, 918
TasI AATT 7 cut(s) 101, 141, 353, 407, 616, 1344, 1531
TatI WGTACW 1 cut(s) 280
TfiI GAWTC 5 cut(s) 370, 608, 656, 1168, 1176
Tru1I TTAA 5 cut(s) 225, 239, 1085, 1188, 1534
Tru9I TTAA 5 cut(s) 225, 239, 1085, 1188, 1534
TscAI CASTG 6 cut(s) 252, 443, 628, 1186, 1383, 1485
TseFI GTSAC 3 cut(s) 111, 550, 1046
TseI GCWGC 1 cut(s) 1124
Tsp45I GTSAC 3 cut(s) 111, 550, 1046
TspDTI ATGAA 6 cut(s) 143, 362, 366, 482, 891, 1385
TspGWI ACGGA 2 cut(s) 203, 349
TspRI CASTG 6 cut(s) 252, 443, 628, 1186, 1383, 1485
Vha464I CTTAAG 1 cut(s) 224
VneI GTGCAC 1 cut(s) 1481
VpaK11BI GGWCC 2 cut(s) 190, 209
XapI RAATTY 3 cut(s) 353, 616, 1344
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
ZrmI AGTACT 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.