MD13G1112700.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001080
Physical Location & Seq
Forward (+)
8183162 .. 8185070
1909 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1112700.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGATTTCTTGAGTTATTGTATTATAATCCCATGTCTTTTATCTCTCTTGTGTTTCTCAGTTCAAGCTCTCAGATCCAATCCCAAAAGGCTTCTTCCACCTGGACCAAAGCCATTTCCCATCATTGGCAATCTCTTGGAGCTCGGCAACAAACCCCATCTCTCTCTCACTAACCTTTCACAACGATACGGCCCCATTATTTCATTGCAACTTGGACAAGTAACCACGGTCGTAGTTTCTTCATCAACCGTGGCAAAGGAGGTCCTCCGAACCCACGACCAGTTCTTGTGCAACCGAACCGTCCCAGACGCAATCCAAGCTTGCGATCTCCGCCAGGACAGCTTGCCCTGGATACCGGTGTCAGAGAAATGGAGAAACCTCCGTAAAATATGCAACAACCAATTGTTTGCAACAAAAGTTCTAGACGCCAACCAAGCCAACCGTCACCTGAAAGTGCAGGAGCTCATCGCCGATGTCAATGAAAGCGTTGTGAAAGGTAAGGCGGTAGAAATTGGGAGGGCTGCTTTTAAGACAACGCTTAATCTGCTGTCGCGTACTGTTTTCTCGGTCGATTTAGCTGACCCGAGAAGTGAGATGGGCAGAGAGTTCAAGGAGATTGTGTGGGGTTTGATGGAAGAGGCAGGGAAGCCAAACTTGGGGGACTATTTTCCTCTGCTTAGGAAACTTGACCCCCAAGGCATTCGACGGCGCATGATCAAACATTTCGTTAAGATTGATTCGCTTTTTGATCGAATGATTACGCAAAGGTTAGAATTGAGAAAATCGAACTATTATGTCACAAGTAATGATATGTTGGATTCTCTCTTGGACGTCAGTGAAGTGAGCAATGAGAATATGGACAAGCTATTGATTGAACATCTGCTTCTGGCTCTATTTGTTGCGGGCACGGATACAACTTCAGCCACGTTGGAATGGGCAATGGCTGAACTACTACGCAACCCGGAAAAACTGTCCAAAGCACAAGAGGAACTAGAGCAAGTCATTGGCAAAGGAAAACCAGTTGAGGAATCGGACATTGCTCAACTCCCTTACTTACAAGCAATAATCAAAGAGACCTTCCGGTTGCATCCGGCTACTCCATTACTACTCCCTCGAAAAGCCGAACAAGACATGGAAATTTGCGGGTACGTTGTCCCAAAGGGTGCACAAGTGCTGGTCAATGCATGGGCTATAGGCAGAGACCCTTGCATTTGGGACAACCCGATCTCATTTGTACCGGAGAGGTTTTTGGGATTAGATGATCAAATCGATGTTTTAGGCAAGAATTTTGAGCTCGTTCCGTTTGGTGGCGGGCGGAGAATATGTCCAGGGATGCTATTGGCAATACGGATGCTAAACTTGATGTTGGGTTCACTTATTAACTCATTTGATTGGAAGCTTGAAGATGGAGTTATACCAGAGAACATGAACATGGAGGAGAAGTTTGGCCTCACCTTACAGATGGCTCATCCTCTAAGGCTTGTGCCAAAAGTCCTAATTAATTTGAAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

505

Amino Acids

56.92

Weight (kDa)

7.04

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 33 - 484 4e-104 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AatII GACGTC 1 cut(s) 834
AccII CGCG 1 cut(s) 553
AciI CCGC 6 cut(s) 331, 503, 902, 1143, 1311, 1315
AclWI GGATC 1 cut(s) 69
AcsI RAATTY 2 cut(s) 1137, 1285
AcuI CTGAAG 1 cut(s) 903
AcyI GRCGYC 2 cut(s) 426, 831
AfaI GTAC 3 cut(s) 556, 1148, 1236
AfiI CCNNNNNNNGG 3 cut(s) 125, 656, 1307
AgeI ACCGGT 1 cut(s) 355
AgsI TTSAA 5 cut(s) 65, 610, 875, 1403, 1507
AjnI CCWGG 4 cut(s) 100, 333, 347, 1327
AjuI GAANNNNNNNTTGG 2 cut(s) 638, 670
AluBI AGCT 9 cut(s) 68, 142, 320, 342, 463, 578, 865, 1294, 1399
AluI AGCT 9 cut(s) 68, 142, 320, 342, 463, 578, 865, 1294, 1399
Alw21I GWGCWC 4 cut(s) 144, 465, 1168, 1296
Alw26I GTCTC 2 cut(s) 1067, 1194
Alw44I GTGCAC 1 cut(s) 1164
AlwI GGATC 1 cut(s) 69
Ama87I CYCGRG 1 cut(s) 583
AoxI GGCC 2 cut(s) 190, 1447
ApaLI GTGCAC 1 cut(s) 1164
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 2 cut(s) 1137, 1285
AseI ATTAAT 1 cut(s) 1500
AsiGI ACCGGT 1 cut(s) 355
AspLEI GCGC 1 cut(s) 711
AspS9I GGNCC 3 cut(s) 104, 191, 262
AsuC2I CCSGG 1 cut(s) 962
AsuHPI GGTGA 2 cut(s) 437, 1444
AvaI CYCGRG 1 cut(s) 583
AvaII GGWCC 2 cut(s) 104, 262
BaeGI GKGCMC 2 cut(s) 908, 1168
BaeI ACNNNNGTAYC 2 cut(s) 344, 377
BanII GRGCYC 3 cut(s) 144, 465, 1296
Bbv12I GWGCWC 4 cut(s) 144, 465, 1168, 1296
BbvI GCAGC 1 cut(s) 508
BccI CCATC 6 cut(s) 128, 165, 589, 625, 1400, 1456
BceAI ACGGC 2 cut(s) 205, 722
BciT130I CCWGG 4 cut(s) 102, 335, 349, 1329
BciVI GTATCC 2 cut(s) 345, 904
BclI TGATCA 2 cut(s) 714, 1261
BcnI CCSGG 1 cut(s) 962
BcoDI GTCTC 2 cut(s) 1067, 1194
BfaI CTAG 2 cut(s) 422, 992
BfmI CTRYAG 1 cut(s) 1191
BfuI GTATCC 2 cut(s) 345, 904
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
Bme1390I CCNGG 5 cut(s) 102, 335, 349, 962, 1329
Bme18I GGWCC 2 cut(s) 104, 262
BmeT110I CYCGRG 1 cut(s) 583
BmgT120I GGNCC 3 cut(s) 104, 191, 262
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 5 cut(s) 102, 335, 349, 962, 1329
BmsI GCATC 3 cut(s) 1096, 1323, 1341
Bpu10I CCTNAGC 1 cut(s) 677
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 1 cut(s) 962
Bsa29I ATCGAT 1 cut(s) 1269
BsaHI GRCGYC 2 cut(s) 426, 831
BsaI GGTCTC 2 cut(s) 1067, 1194
BsaJI CCNNGG 5 cut(s) 225, 249, 347, 694, 1328
BsaWI WCCGGW 3 cut(s) 355, 1080, 1237
BsaXI ACNNNNNCTCC 6 cut(s) 605, 635, 1309, 1339, 1427, 1457
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 656, 1307
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 2 cut(s) 280, 1019
Bse3DI GCAATG 4 cut(s) 203, 853, 945, 1035
BseBI CCWGG 4 cut(s) 102, 335, 349, 1329
BseCI ATCGAT 1 cut(s) 1269
BseDI CCNNGG 5 cut(s) 225, 249, 347, 694, 1328
BseGI GGATG 4 cut(s) 1087, 1338, 1356, 1468
BseLI CCNNNNNNNGG 3 cut(s) 125, 656, 1307
BseMI GCAATG 4 cut(s) 203, 853, 945, 1035
BseMII CTCAG 2 cut(s) 72, 85
BseNI ACTGG 2 cut(s) 280, 1019
BseRI GAGGAG 1 cut(s) 1451
BseSI GKGCMC 2 cut(s) 908, 1168
BseXI GCAGC 1 cut(s) 508
BsgI GTGCAG 1 cut(s) 476
Bsh1236I CGCG 1 cut(s) 553
Bsh1285I CGRYCG 2 cut(s) 231, 570
BshFI GGCC 2 cut(s) 192, 1449
BshTI ACCGGT 1 cut(s) 355
BshVI ATCGAT 1 cut(s) 1269
BsiEI CGRYCG 2 cut(s) 231, 570
BsiHKAI GWGCWC 4 cut(s) 144, 465, 1168, 1296
BsiHKCI CYCGRG 1 cut(s) 583
BsiSI CCGG 5 cut(s) 356, 962, 1081, 1091, 1238
BslFI GGGAC 4 cut(s) 287, 674, 1139, 1229
BslI CCNNNNNNNGG 3 cut(s) 125, 656, 1307
BsmAI GTCTC 2 cut(s) 1067, 1194
BsmFI GGGAC 4 cut(s) 287, 674, 1139, 1229
BsmI GAATGC 1 cut(s) 699
BsnI GGCC 2 cut(s) 192, 1449
Bso31I GGTCTC 2 cut(s) 1067, 1194
BsoBI CYCGRG 1 cut(s) 583
Bsp1286I GDGCHC 5 cut(s) 144, 465, 908, 1168, 1296
Bsp143I GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
BspACI CCGC 6 cut(s) 331, 503, 902, 1143, 1311, 1315
BspANI GGCC 2 cut(s) 192, 1449
BspCNI CTCAG 2 cut(s) 71, 84
BspDI ATCGAT 1 cut(s) 1269
BspFNI CGCG 1 cut(s) 553
BspLI GGNNCC 1 cut(s) 193
BspPI GGATC 1 cut(s) 69
BspTNI GGTCTC 2 cut(s) 1067, 1194
BsrDI GCAATG 4 cut(s) 203, 853, 945, 1035
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 2 cut(s) 280, 1019
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 5 cut(s) 225, 249, 347, 694, 1328
BssMI GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
BssNI GRCGYC 2 cut(s) 426, 831
BssT1I CCWWGG 1 cut(s) 694
Bst2UI CCWGG 4 cut(s) 102, 335, 349, 1329
Bst4CI ACNGT 6 cut(s) 229, 250, 301, 443, 559, 972
Bst6I CTCTTC 1 cut(s) 630
BstACI GRCGYC 2 cut(s) 426, 831
BstC8I GCNNGC 4 cut(s) 322, 344, 904, 1313
BstDEI CTNAG 4 cut(s) 58, 71, 677, 1475
BstDSI CCRYGG 2 cut(s) 225, 249
BstF5I GGATG 4 cut(s) 1087, 1338, 1356, 1468
BstFNI CGCG 1 cut(s) 553
BstHHI GCGC 1 cut(s) 711
BstKTI GATC 6 cut(s) 77, 328, 717, 751, 1227, 1264
BstMAI GTCTC 2 cut(s) 1067, 1194
BstMBI GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
BstMCI CGRYCG 2 cut(s) 231, 570
BstMWI GCNNNNNNNGC 5 cut(s) 317, 330, 339, 434, 544
BstNI CCWGG 4 cut(s) 102, 335, 349, 1329
BstSCI CCNGG 5 cut(s) 100, 333, 347, 960, 1327
BstSFI CTRYAG 1 cut(s) 1191
BstSLI GKGCMC 2 cut(s) 908, 1168
BstUI CGCG 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 508
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 1269
BsuI GTATCC 2 cut(s) 345, 904
BsuRI GGCC 2 cut(s) 192, 1449
BsuTUI ATCGAT 1 cut(s) 1269
BtgI CCRYGG 2 cut(s) 225, 249
BtgZI GCGATG 1 cut(s) 451
BtsCI GGATG 4 cut(s) 1087, 1338, 1356, 1468
BtsIMutI CAGTG 1 cut(s) 841
Cac8I GCNNGC 4 cut(s) 322, 344, 904, 1313
CfoI GCGC 1 cut(s) 711
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 3 cut(s) 104, 191, 262
ClaI ATCGAT 1 cut(s) 1269
CseI GACGC 2 cut(s) 317, 434
Csp6I GTAC 3 cut(s) 555, 1147, 1235
CspAI ACCGGT 1 cut(s) 355
CviAII CATG 6 cut(s) 33, 712, 1132, 1185, 1426, 1432
CviQI GTAC 3 cut(s) 555, 1147, 1235
DdeI CTNAG 4 cut(s) 58, 71, 677, 1475
DpnI GATC 6 cut(s) 76, 327, 716, 750, 1226, 1263
DpnII GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 2 cut(s) 320, 1330
Ecl136II GAGCTC 3 cut(s) 142, 463, 1294
Eco130I CCWWGG 1 cut(s) 694
Eco24I GRGCYC 3 cut(s) 144, 465, 1296
Eco31I GGTCTC 2 cut(s) 1067, 1194
Eco47I GGWCC 2 cut(s) 104, 262
Eco53kI GAGCTC 3 cut(s) 142, 463, 1294
Eco57I CTGAAG 1 cut(s) 903
Eco88I CYCGRG 1 cut(s) 583
EcoICRI GAGCTC 3 cut(s) 142, 463, 1294
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 4 cut(s) 100, 333, 347, 1327
EcoT14I CCWWGG 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 3 cut(s) 144, 465, 1296
ErhI CCWWGG 1 cut(s) 694
FaeI CATG 6 cut(s) 36, 715, 1135, 1188, 1429, 1435
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FaqI GGGAC 4 cut(s) 287, 674, 1139, 1229
FatI CATG 6 cut(s) 32, 711, 1131, 1184, 1425, 1431
FauI CCCGC 3 cut(s) 895, 1136, 1304
FbaI TGATCA 2 cut(s) 714, 1261
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 4 cut(s) 1074, 1345, 1363, 1455
FriOI GRGCYC 3 cut(s) 144, 465, 1296
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 2 cut(s) 422, 992
GlaI GCGC 1 cut(s) 710
GluI GCNGC 1 cut(s) 522
HaeIII GGCC 2 cut(s) 192, 1449
HapII CCGG 5 cut(s) 356, 962, 1081, 1091, 1238
HgaI GACGC 2 cut(s) 317, 434
HhaI GCGC 1 cut(s) 711
Hin1I GRCGYC 2 cut(s) 426, 831
Hin1II CATG 6 cut(s) 36, 715, 1135, 1188, 1429, 1435
Hin6I GCGC 1 cut(s) 709
HinP1I GCGC 1 cut(s) 709
HindIII AAGCTT 2 cut(s) 318, 1397
HinfI GANTC 3 cut(s) 737, 818, 1028
HpaII CCGG 5 cut(s) 356, 962, 1081, 1091, 1238
HphI GGTGA 2 cut(s) 437, 1444
Hpy166II GTNNAC 2 cut(s) 1166, 1373
Hpy188I TCNGA 4 cut(s) 74, 269, 364, 1033
Hpy188III TCNNGA 2 cut(s) 10, 422
Hpy8I GTNNAC 2 cut(s) 1166, 1373
Hpy99I CGWCG 1 cut(s) 708
HpyAV CCTTC 1 cut(s) 1087
HpyCH4III ACNGT 6 cut(s) 229, 250, 301, 443, 559, 972
HpyCH4IV ACGT 3 cut(s) 831, 926, 1149
HpyCH4V TGCA 9 cut(s) 208, 291, 393, 410, 457, 1087, 1166, 1184, 1209
HpyF10VI GCNNNNNNNGC 5 cut(s) 317, 330, 339, 434, 544
HpyF3I CTNAG 4 cut(s) 58, 71, 677, 1475
HpySE526I ACGT 3 cut(s) 831, 926, 1149
Hsp92I GRCGYC 2 cut(s) 426, 831
Hsp92II CATG 6 cut(s) 36, 715, 1135, 1188, 1429, 1435
HspAI GCGC 1 cut(s) 709
Ksp22I TGATCA 2 cut(s) 714, 1261
Kzo9I GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
LmnI GCTCC 2 cut(s) 139, 460
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 3 cut(s) 1096, 1323, 1341
MaeI CTAG 2 cut(s) 422, 992
MaeII ACGT 3 cut(s) 831, 926, 1149
MaeIII GTNAC 3 cut(s) 220, 443, 796
MalI GATC 6 cut(s) 76, 327, 716, 750, 1226, 1263
MboI GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
MboII GAAGA 4 cut(s) 86, 231, 647, 1415
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 5 cut(s) 144, 465, 908, 1168, 1296
MluCI AATT 7 cut(s) 401, 510, 773, 1137, 1285, 1497, 1501
MmeI TCCRAC 2 cut(s) 795, 909
Mph1103I ATGCAT 1 cut(s) 1186
MseI TTAA 5 cut(s) 528, 540, 729, 1380, 1500
MslI CAYNNNNRTG 1 cut(s) 1430
MspI CCGG 5 cut(s) 356, 962, 1081, 1091, 1238
MspR9I CCNGG 5 cut(s) 102, 335, 349, 962, 1329
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 699
MvaI CCWGG 4 cut(s) 102, 335, 349, 1329
MvnI CGCG 1 cut(s) 553
MwoI GCNNNNNNNGC 5 cut(s) 317, 330, 339, 434, 544
NciI CCSGG 1 cut(s) 962
NdeII GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
NlaIII CATG 6 cut(s) 36, 715, 1135, 1188, 1429, 1435
NlaIV GGNNCC 1 cut(s) 193
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 2 cut(s) 443, 796
NsiI ATGCAT 1 cut(s) 1186
PctI GAATGC 1 cut(s) 699
PfeI GAWTC 3 cut(s) 737, 818, 1028
PinAI ACCGGT 1 cut(s) 355
PkrI GCNGC 1 cut(s) 523
PpuMI RGGWCCY 1 cut(s) 262
PshBI ATTAAT 1 cut(s) 1500
PsiI TTATAA 1 cut(s) 26
Psp124BI GAGCTC 3 cut(s) 144, 465, 1296
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 4 cut(s) 100, 333, 347, 1327
PspGI CCWGG 4 cut(s) 100, 333, 347, 1327
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 3 cut(s) 104, 191, 262
PspPPI RGGWCCY 1 cut(s) 262
PsuI RGATCY 1 cut(s) 74
RsaI GTAC 3 cut(s) 556, 1148, 1236
RsaNI GTAC 3 cut(s) 555, 1147, 1235
RseI CAYNNNNRTG 1 cut(s) 1430
SacI GAGCTC 3 cut(s) 144, 465, 1296
SaqAI TTAA 5 cut(s) 528, 540, 729, 1380, 1500
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 6 cut(s) 74, 325, 714, 748, 1224, 1261
Sau96I GGNCC 3 cut(s) 104, 191, 262
ScrFI CCNGG 5 cut(s) 102, 335, 349, 962, 1329
SduI GDGCHC 5 cut(s) 144, 465, 908, 1168, 1296
SfaNI GCATC 3 cut(s) 1096, 1323, 1341
SfcI CTRYAG 1 cut(s) 1191
SinI GGWCC 2 cut(s) 104, 262
SmiMI CAYNNNNRTG 1 cut(s) 1430
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 7 cut(s) 401, 510, 773, 1137, 1285, 1497, 1501
SsiI CCGC 6 cut(s) 331, 503, 902, 1143, 1311, 1315
SspMI CTAG 2 cut(s) 422, 992
SstI GAGCTC 3 cut(s) 144, 465, 1296
StyD4I CCNGG 5 cut(s) 100, 333, 347, 960, 1327
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 6 cut(s) 229, 250, 301, 443, 559, 972
TaiI ACGT 3 cut(s) 834, 929, 1152
TaqI TCGA 6 cut(s) 570, 703, 751, 785, 1114, 1269
TaqII GACCGA 1 cut(s) 556
TasI AATT 7 cut(s) 401, 510, 773, 1137, 1285, 1497, 1501
TfiI GAWTC 3 cut(s) 737, 818, 1028
Tru1I TTAA 5 cut(s) 528, 540, 729, 1380, 1500
Tru9I TTAA 5 cut(s) 528, 540, 729, 1380, 1500
TscAI CASTG 1 cut(s) 841
TseFI GTSAC 2 cut(s) 443, 796
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 443, 796
TspDTI ATGAA 4 cut(s) 192, 231, 495, 1442
TspGWI ACGGA 4 cut(s) 371, 923, 1290, 1363
TspRI CASTG 1 cut(s) 841
VneI GTGCAC 1 cut(s) 1164
VpaK11BI GGWCC 2 cut(s) 104, 262
VspI ATTAAT 1 cut(s) 1500
XapI RAATTY 2 cut(s) 1137, 1285
XbaI TCTAGA 1 cut(s) 421
XspI CTAG 2 cut(s) 422, 992
ZraI GACGTC 1 cut(s) 832
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.